The inVention relates to cultiVation medium technologies, in particular to a dendrobium officinale cultiVation medium and a preparation method thereof. The medium is prepared from, by weight, 10-15 parts of shaddock peel, 3-7 parts of momordica charantia, 12-17 parts of taraxacum, 3-6 parts of edible fungi, 2-5 parts of paper mulberry bark, 7-10 parts of the bark of eucommia, 4-8 parts of papaya peel, 3-7 parts of complex enzyme, 5-9 parts of purple sweet potato, 3-7 parts of ardealite, 1-2 parts of carrageenan, 1-2 parts of pectin, 1-2 parts of locust bean gum and 140-160 parts of water. Thedendrobium officinale cultiVation medium has the adVantages that compared with an existing medium, the water permeability, gas permeability and water retaining capacity are better; the obserVation isconVenient; mildew is not easy, and injurious insects do not hide easily; not only can surViVal rate and pesticide effect stability of the dendrobium officinale cultiVation effectiVely improVed, but also the growth situations of the dendrobium officinale can be obserVed conVeniently.