Coupling isoelectric focusing-based fractionation with mass spectrometry analysis
The method of capillary isoelectric focusing combined with desalting size exclusion chromatography and mass spectrometry addresses the limitations of conventional techniques by providing high-resolution and high-throughput characterization of charge variants in therapeutic proteins, enabling site-specific modification identification.
Patent Information
- Application Number
- AU2022303362
- Authority / Receiving Office
- AU · AU
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2022-01-20
- Filing Date
- 2022-06-29
- Publication Date
- 2026-08-27
- Estimated Expiration
- 2042-06-29
AI Technical Summary
Conventional methods for characterizing charge variants of therapeutic proteins are limited in resolution and throughput, particularly in identifying site-specific protein modifications associated with charge variants, due to incompatibilities between isoelectric focusing and mass spectrometry analysis.
A method involving capillary isoelectric focusing followed by high-throughput fraction collection and desalting size exclusion chromatography, allowing for offline processing of fractions, which are then analyzed by mass spectrometry to identify specific post-translational modifications corresponding to each charge variant.
Enables high-resolution, high-throughput analysis of site-specific protein modifications, improving the characterization of therapeutic proteins and optimizing production processes by validating and enhancing protein biophysical characteristics and homogeneity.