Method for stabilizing hemoglobin and reagents for performing the same

Abstract

1006789503 20 26 21 40 68 07 A ug 2 02 6 A B S T R A C T 2 0 2 6 2 1 4 0 6 8 0 7 2 0 2 6 A u g 1 0 0 6 7 8 9 5 0 3

Description

CROSS-REFERENCING This application is a divisional of Australian patent application no. 2024216400 which in turn is a divisional application of Australian patent application no. 2019242190, which claims the benefit of U.S. provisional application serial nos. 62 / 648,874, filed on Mar 27, 2018, and 62 / 685,248, filed on Jun 14, 2018, all of which are incorporated by reference herein. BACKGROUND Tests that detect hemoglobin (Hb) in a stool specimen are commonly used for colorectal cancer screening. The common procedures utilize a sample collection device that contains buffer to stabilize hemoglobin from the time of collection until the detection assay is conducted at the lab. The ability of the buffer to properly stabilize hemoglobin is critical for accurate test results and must stabilize hemoglobin during shipment over a range of environmental conditions. Increasing the ability of the buffer to stabilize hemoglobin would, for example, allow longer shipping durations and provide more flexibility to the patient and test lab for sample processing. SUMMARY Provided herein, among other things, is a stool resuspension solution that comprises a hemoglobin stabilization reagent. In some embodiments, the hemoglobin stabilization reagent in the solution may be an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, or an HRP stabilization component and, optionally, a polyvalent cation. The solution finds use in methods that may benefit from stabilizing hemoglobin. In some embodiments, this method may comprise combining the stool sample with the stool resuspension solution to produce a suspension, and maintaining the suspension for a period of time. In some embodiments, this method may comprise shipping the suspension to a remote location. The hemoglobin stabilization reagent in the solution stabilizes the hemoglobin, thereby allowing the hemoglobin in the sample to be more accurately measured after several days in transit. Compositions, methods and devices that employ the stool resuspension solution are provided. 1006789503 2026214068   07 Aug 2026 DESCRIPTION OF EXEMPLARY EMBODIMENTS Before the present invention is described in greater detail, it is to be understood that this invention is not limited to particular embodiments described, as such may, of course, 5 vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present invention will be limited only by the appended claims. Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between 10 the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the invention, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included 15 limits are also included in the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present invention, the preferred methods and 20 materials are now described. All publications and patents cited in this specification are herein incorporated by reference as if each individual publication or patent were specifically and individually indicated to be incorporated by reference and are incorporated herein by reference to disclose and describe the methods and / or materials in connection with which the publications are 25 cited. The citation of any publication is for its disclosure prior to the filing date and should not be construed as an admission that the present invention is not entitled to antedate such publication by virtue of prior invention. Further, the dates of publication provided may be different from the actual publication dates which may need to be independently confirmed. It must be noted that as used herein and in the appended claims, the singular forms 30   “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise. It is further noted that the claims may be drafted to exclude any optional element. As such, this statement is intended to serve as antecedent basis for use of such exclusive terminology as “solely,” “only” and the like in connection with the recitation of claim elements, or use of a “negative” limitation. 2026214068   07 Aug 2026 As will be apparent to those of skill in the art upon reading this disclosure, each of the individual embodiments described and illustrated herein has discrete components and features which may be readily separated from or combined with the features of any of the other several embodiments without departing from the scope or spirit of the present invention. Any 5 recited method can be carried out in the order of events recited or in any other order which is logically possible. A stool resuspension solution comprising one or more hemoglobin stabilization reagents is provided. In some embodiments, the one or more hemoglobin stabilization 10 reagents may be selected from, e.g., an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin and an HRP stabilization component and, optionally, a polyvalent cation. Examples and concentrations of such reagents in the solution are set forth in the tables shown below and in the appendices. In some embodiments, the solution may comprise an osmolyte, e.g., betaine. In these 15 embodiments, the osmolyte (e.g., betaine) may be at a concentration in the range of 2 M to 5 M. In some embodiments, the solution may comprise a sugar, e.g., sucrose or trehalose. In these embodiments, the sugar (e.g., sucrose or trehalose) may be at a concentration in the range of 0.1 M to 0.5 M. In either case, the solution may optionally contain a polyvalent 20 cation, Mg2+ or Ca2+. In these embodiments, the polyvalent cation (e.g., calcium or magnesium ions) may be at a concentration in the range of 5 mM to 25 mM. In some embodiments, the solution may comprise a polysaccharide, e.g., a substituted or unsubstituted polygalacturonic acid such as a-( 1^4)-linked D-galacturonic acid. In these embodiments, the polysaccharide (e.g., the substituted or unsubstituted polygalacturonic acid) may be at a concentration in the range of 0.005% to 0.5% (e.g., 0.01% to 0.125%). In these embodiments, the solution may optionally contain a polyvalent cation. In embodiments in which the solution contains a polyvalent cation, the polyvalent cation may be at a concentration in the range of 5 mM to 25 mM. In some embodiments, the solution may comprise substituted or unsubstituted polygalacturonic acid and a multivalent cation (e.g., a calcium salt or magnesium salt) at a concentration in the range of 5 mM to 25 mM. In some embodiments, the solution may comprise a protoporphyrin, e.g., a protoporphyrin IX or an analog thereof such as octaethylporphyrin (H2OEP) or tetraphenylporphyrin (H2TPP), complexed with a metal ion. In these embodiments, the protoporphyrin may be hemin (protoporphyrin IX containing a ferric iron (Fe3+) ion with 2026214068   07 Aug 2026 a coordinating chloride ligand) or hematin. In other embodiments, the protoporphyrin may be protoporphyrin IX complexed with a divalent or trivalent cation (e.g., Zn2+, Cr3+, or Co3+, for example). In these embodiments, the protoporhyrin may in the solution at a concentration in the range of 0.1 pM to 100 pM (e.g., 1 pM to 10 pM). In some embodiments, the solution may comprise an HRP stabilization component selected from HRP Conjugate Stabilizer (PN 85R-102; Fitzgerald Industries), HRP Conjugate Stabilizer (PN SZ02; Surmodics) and HRP Conjugate Stabilizer (PN abl71537; Abeam). Other HRP stabilization components, e.g.,, AbGuard (BioRad PN: BUF052; BioRad Laboratories Inc. 2000 Alfred Nobel Dr, Hercules, CA 94547) can potentially be used. If the solution comprises an HRP stabilization component, then the component may be at a concentration in the range of 1% to 20%, e.g., 5% to 15% or 5% to 20%. In any embodiment, the solution may comprise a polyvalent cation, e.g., calcium or magnesium ions. In these embodiments, the polyvalent cation may be at a concentration in the range of 5 mM to 25 mM. In any embodiment, the solution further comprises Tris buffer (e.g., 10 mM to 50 mM Tris, pH 7.5), bovine serum albumen (e.g., 5% to 20% BSA), polysorbate 20 (e.g., 0.05% to 0.2% polysorbate 20), a preservative such as sodium azide (e.g., 0.05% to 0.2% sodium azide), a salt such as sodium chloride (e.g., 50 mM to 250 mM sodium chloride), a chelator such as ethylenediaminetetraacetic acid (e.g., 5 mM to 20 mM ethylenediaminetetraacetic acid), and an antibiotic such as gentamicin (e.g., 5 ug / mL to 50 ug / mL). A composition comprising: (a) a stool sample and (b) the stool resuspension solution, as described above, is also provided. The stool sample may be suspended in the solution. Some components of the stool may be dissolved in the solution, where other, insoluble components, may be suspended but not dissolved in the solution. In some embodiments, the stool sample may contain hemoglobin which may be dissolved in the solution. In these embodiments, the presence of the hemoglobin stabilization reagent may increase the amount of hemoglobin in the sample after it has been subjected to a real or simulated shipping stress, as described below, as compared to an otherwise identical control sample that has not been subjected to the stress, e.g., a “To” sample. In some embodiments, use of the hemoglobin stabilization reagent may increase the amount of hemoglobin in the sample by at least 10%, at least 20 or at least 30%, relative to a control stool sample that has been suspended in an otherwise identical solution that does not contain the hemoglobin stabilization reagent. The amount of hemoglobin in the sample may be determined by enzyme-linked immunosorbent assay (ELISA) although several other methods are known (see, generally, Haug et al, Am J. 2026214068   07 Aug 2026 Gastroenterology 105: 682-690; Ahlquist et al, Ann Intern Med 1984;101:297-302; Ahlquist et al, JAMA 1993 269:1262-1267; Young et al Dig Dis Sei. 2015 60: 609-622; and Harewood et al Mayo Clin. Proc. 2002 77: 23-28). Also provided is a method of stabilizing stool hemoglobin. In some embodiments, this method may comprise combining the stool sample with the stool resuspension solution as described above, i.e., a solution comprising one or more hemoglobin stabilization reagents selected from: an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation; a protoporphyrin; and an HRP stabilization component and, optionally, a polyvalent cation, to produce a suspension; and maintaining the suspension for a period of time. Details of the identities of the reagents that can be in the solution as well as their concentrations are described above, below and in the accompanying appendices. In some embodiments, the method may comprise obtaining the stool sample from a larger mass of stool by scraping or scooping, e.g., using a stool sampling implement. In some embodiments, the method may comprise shipping the sample to a remote location, e.g., to another town, city, state or country, for testing. In these embodiments, the sample may be waiting to be shipped, en route, or waiting to be tested at a remote location for several days, e.g., 3, 4, 5, 6, or 7 days, thereby subjecting the sample to stresses that potentially reduce the amount of hemoglobin in the sample. Also provided is a method that comprises receiving a composition that comprises: (a) a stool sample and (b) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from, e.g., an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, and an HRP stabilization component and, optionally, a polyvalent cation. Examples and concentrations of such reagents in the solution are set forth above, below and in the accompanying appendices. In certain embodiments, this method may further comprise performing an assay on the sample after it has been received. For example, the concentration of hemoglobin in the solution may be measured, methods for performing which are described above. In addition, the amount of one or more colorectal cancer tumor markers in the sample may be tested after it has been received (see, generally, Lech et al, World J Gastroenterol. 2016 22: 1745-1755 and Scheruders et al, Curr. Treat. Options Gastroenterol. 2016 14: 152-162). These embodiments may further comprise forwarding test results to a remote location by, e.g., fax or e-mail, etc., or via the internet via a portal, so that the results can be obtained by a medical practitioner (e.g., an MD or a nurse, etc.), in advance of providing a diagnosis to the patient from which the stool sample was obtained. 2026214068   07 Aug 2026 Also provided is a sample collection device that comprises: (a) a sample collection container having an open end; (b) a stool resuspension solution that is within the container, where the solution comprises one or more hemoglobin stabilization reagents selected from: an osmolyte, a polyvalent cation, a sugar or polysaccharide and, optionally, a polyvalent cation, a protoporphyrin, and an HRP stabilization component and, optionally, a polyvalent cation, as described herein; and (c) a stool sampling rod comprising a distal beveled tip for scooping and / or scraping a sample of stool. In this device, the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device. In some embodiments, the sample collection container and sampling rod may connect via a screw fit. With the exception of the hemoglobin stabilization reagent, details, options and the design of an example of such a sample collection device can be found in US9211112, for example. In some embodiments, the device may comprise a) a body comprising a sample collection chamber bounded on a distal end by a penetrable seal affixed to a recessed sealing surface of the distal end of the body and bounded on a proximal end by a septum comprising an aperture, said penetrable seal being penetrable by a pipette tip or needle; b) a flexible sampling rod comprising a proximal portion and a distal portion, said sampling rod adapted to fit through and seal said aperture when said distal portion is in said sample collection chamber, wherein said distal portion comprises: i) an asymmetrical beveled tip at a distal end, said beveled tip having an apex at the circumference of the flexible sampling rod and configured to bend the flexible sampling rod away from collisions and / or to deflect collisions with an inserted pipette tip or needle; and ii) a plurality of stacked coaxial frusta of cones to form a plurality of metering ridges; and c) the stool resuspension solution described above in the sample collection chamber, as described in US9211112. EMBODIMENTS Embodiment 1. A composition comprising: (a) a stool sample; and (b) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation. Embodiment 2. The composition of embodiment 1, wherein the stool sample contains hemoglobin. 2026214068   07 Aug 2026 Embodiment 3. The composition of embodiment 1, wherein the solution comprises betaine. Embodiment 4. The composition of embodiment 2, wherein the betaine is at a concentration in the range of 2 M to 5 M. Embodiment 5. The composition of embodiment 1, wherein the solution comprises sucrose or trehalose, and, optionally, a polyvalent cation. Embodiment 6. The composition of embodiment 5, wherein the sucrose or trehalose is at a concentration in the range of 0.1 M to 0.5 M and the solution, optionally comprises, a polyvalent cation. Embodiment 7. The composition of embodiment 1, wherein the solution comprises substituted or unsubstituted polygalacturonic acid and, optionally, a polyvalent cation. Embodiment 8. The composition of embodiment 1 or 7, wherein the solution comprises a-(l-4)-linked D-galacturonic acid and, optionally, a polyvalent cation. Embodiment 9. The composition of embodiment 7, wherein the substituted or unsubstituted polygalacturonic acid is at a concentration in the range of 0.005% to 0.5%. Embodiment 10. The composition of embodiment 1, wherein the solution comprises substituted or unsubstituted polygalacturonic acid and a multivalent cation (e.g., a calcium salt or magnesium salt) at a concentration in the range of 5 mM to 25 mM. Embodiment 11. The composition of embodiment 1, wherein the solution comprises a protoporphyrin. Embodiment 12. The composition of embodiment 11, wherein the protoporphyrin is hemin or hematin. Embodiment 13. The composition of embodiment 11, wherein the protoporphyrin comprises protoporphyrin IX complexed with a divalent or trivalent cation (e.g., Zn2+, Cr3+ or Co3+). Embodiment 14. The composition of any of embodiments 11-13, wherein the protoporphyrin is at a concentration in the range of 0.1 pM to 100 pM (e.g., 1 pM to 10 pM). Embodiment 15. The composition of embodiment 1, wherein the solution comprises an HRP stabilization component selected from HRP Conjugate Stabilizer (PN 85R-102; Fitzgerald Industries), HRP Conjugate Stabilizer (PN SZ02; Surmodics) and HRP Conjugate Stabilizer (PN abl71537; Abeam) Embodiment 16. The composition of embodiment 15, wherein the HRP stabilization component is at a concentration in the range of 1% to 20%. 2026214068   07 Aug 2026 Embodiment 17. The composition of any prior embodiment, wherein the solution comprises one or more cations selected from iron, cobalt, chromium, zinc, calcium or magnesium ions. Embodiment 18. The composition of embodiment 17, wherein the cations are at a concentration in the range of 5 mM to 25 mM. Embodiment 19. The composition of any prior embodiment, wherein the stool sample is suspended in the solution. Embodiment 20. The composition of any prior embodiment, wherein the solution further comprises Tris buffer, bovine serum albumen, polysorbate 20, sodium azide, sodium chloride, ethylenediaminetetraacetic acid, and gentamicin. Embodiment 21. A method of stabilizing hemoglobin in a stool sample, comprising: combining the stool sample with a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation; to produce a suspension; and maintaining the suspension for a period of time. Embodiment 22. The method of embodiment 17, wherein the method comprises shipping the suspension to a remote location. Embodiment 23. A method of analyzing a stool sample, comprising: (a) receiving, from a remote location, a composition comprising: (i) a stool sample; and (ii) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation, wherein the stool sample is suspended in the solution; and (b) measuring the amount of hemoglobin in the composition. Embodiment 24. The method of embodiment 19, further comprising measuring the amount of one or more colorectal cancer tumor markers in the sample. Embodiment 25. A sample collection device comprising: a sample collection container having an open end; a stool resuspension solution that is within the container, comprising one or more hemoglobin stabilization reagents selected from: a protoporphyrin; a polyvalent cation; a sugar or polysaccharide and, optionally, a polyvalent cation; an osmolyte; and an HRP stabilization component and, optionally, a polyvalent cation; and a stool sampling rod comprising a distal beveled tip for scooping and / or scraping a sample of stool, wherein the distal end of the sampling rod is dimensioned to be inserted into the sample 2026214068   07 Aug 2026 collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device. Embodiment 26. The sample collection device of embodiment 25, wherein the sample collection container and sampling rod connect via a screw fit. Embodiment 27. A method of stabilizing hemoglobin in a stool sample, comprising: combining the stool sample with a stool resuspension solution comprising protoporphyrin IX complexed with a multivalent cation to produce a suspension; and maintaining the suspension for a period of time. Embodiment 28. The method of embodiment 27, wherein the method comprises shipping the suspension to a remote location. Embodiment 29. A method of analyzing a stool sample, comprising: (a) receiving, from a remote location, a composition comprising: (i.) a stool sample; and (ii.) a stool resuspension solution comprising protoporphyrin IX complexed with a multivalent cation, wherein the stool sample is suspended in the solution; and (b) measuring the amount of hemoglobin in the composition. Embodiment 30. The method of any of embodiments 27-29, further comprising measuring the amount of one or more colorectal cancer tumor markers in the sample. Embodiment 31. The method of any of embodiments 27-30, wherein the multivalent cation is Cr3+ or Co3+. Embodiment 32. The method of any of embodiments 27-31, wherein the solution has a pH in the range of pH 6.5 to pH 7.4. Embodiment 33. The method of any of embodiments 27-32, wherein the multivalent cation is Cr3+ and the pH of the solution is in the range of pH 6.9 to pH 7.4. Embodiment 34. The method of any of embodiments 27-32, wherein the multivalent cation is Co3+ and the pH of the solution is in the range of pH 6.5 to pH 7.0. Embodiment 35. The method of any of embodiments 27-34, wherein the protoporphyrin IX is at a concentration in the range of 0.5 uM to 10 uM. Embodiment 36. A sample collection device comprising: a sample collection container having an open end; a stool resuspension solution comprising protoporphyrin IX complexed with a multivalent cation; and a stool sampling rod comprising a distal beveled tip for scooping and / or scraping a sample of stool, wherein the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the 2026214068   07 Aug 2026 sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device. Embodiment 37. The sample collection device of embodiment 36, wherein the sample collection container and sampling rod connect via a screw fit. Embodiment 38. The sample collection device of embodiment 36 or 37, wherein the multivalent cation is Cr3+ or Co3+. Embodiment 39. The sample collection device of any of embodiments 36-38, wherein the solution has a pH in the range of pH 6.5 to pH 7.4. Embodiment 40. The sample collection device of any of embodiments 36-39, wherein the multivalent cation is Cr3+ and the pH of the solution is in the range of pH 6.9 to pH 7.4. Embodiment 41. The sample collection device of any of embodiments 36-39, wherein the multivalent cation is Co3+ and the pH of the solution is in the range of pH 6.5 to pH 7.0. Embodiment 42. The sample collection device of any of embodiments 16-21, wherein the protoporphyrin IX is at a concentration in the range of 0.5 uM to 10 uM. Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, it is readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto without departing from the spirit or scope of the 5 appended claims. EXAMPLE 1 SIMULATED SHIPPING STRESS To assess the stability of hemoglobin in stool samples, samples were exposed to simulated 10 shipping stress conditions using a thermal cycler. Table 1 shows the time and temperature profile for a 3-Day (72 hour) shipping simulation. An ELISA based detection method is used to determine the hemoglobin concentrations of samples before and after exposure to the shipping simulation. Hemoglobin stability is calculated as a % recovery of hemoglobin after the samples have been exposed to the shipping simulation. Table 1. 3-Day Shipping Simulation Simulated Shipping Condition Duration (hrs) Total time (hrs) Temp (°C) Pre-shipping hold at ambient temperature 24 24 22 Ambient, overnight shipping 9 33 22 3 36 35 2026214068   07 Aug 2026 Table 1. 3-Day Shipping Simulation Simulated Shipping Condition Duration (hrs) Total time (hrs) Temp (°C) 4 40 22 5 45 35 5 50 22 4 54 35 Post-shipping hold at ambient temperature 18 72 22 An accelerated shipping simulation profile was created to mimic the 3-Day shipping simulation but in a shortened amount of time. Table 2 shows the time and temperature profile for an accelerated shipping simulation. Samples exposed to the accelerated shipping simulation exhibit 5 similar levels of Hb stability as when exposed to the 3-Day shipping simulation. Table 2. Accelerated Shipping Simulation Simulated Shipping Condition Duration (hrs) Temp (°C) Incubation at Temperature 16 35 10           To assess the stability of hemoglobin in stool samples beyond 3 days, a 7-Day shipping simulation was created. Table 3 shows the time and temperature profile for a 7-Day shipping simulation. Table 3. 7-Day Shipping Simulation Simulated Shipping Condition Duration (hrs) Total time (hrs) Temp (°C) Pre-shipping hold at ambient temperature 40 40 22 Ambient, overnight shipping 9 49 22 3 52 35 4 56 22 5 61 35 5 66 22 4 70 35 Post-shipping hold at ambient temperature 2 72 22 48 120 22 48 168 22 2026214068   07 Aug 2026 As an additional challenge, a shipping simulation meant to represent a “worst case” scenario was created using extreme temperatures. Table 4 show the time and temperature profile for a 3-Day extreme temperature shipping simulation. Table 4. 3-Day Extreme Temperature Shipping Simulation Simulated Shipping Condition Duration (hrs) Total time (hrs) Temp (°C) Pre-shipping hold at ambient temperature 16 16 22 Extreme High Temperature overnight shipping 2.17 18.17 43.5 6.17 24.34 33 5 29.34 34 1.83 31.17 32.5 1.5 32.67 27.5 1.67 34.34 38.5 2.83 37.17 33 2.83 40 37 Post-shipping hold at ambient temperature 32 72 22 ASSAY RESULTS The performance of the existing buffer formulation (shown below) was tested. The average percent hemoglobin recovery across all samples was determined for days 3,5, and 7 10 of the 7-Day shipping simulation. These results are shown below. Final Concentration Component N / A Water 20 mM 1 M Tris pH 7.5 10% BSA 0.10% 20% Tween 20 0.095% 5% Sodium Azide 140 mM Sodium Chloride 10 mM 0.5M EDTA 15 pg / mL 50 mg / mL Gentamicin Results are listed in the table below. Timepoint % Hb Recovery Day 3 71 Day 5 68 Day 7 63 2026214068   07 Aug 2026 The desired % Hb Recovery at 7 days is minimally 70%. As such, the existing buffer formulation does not achieve the desired level of stability beyond 3 days (72 hours). In an effort to improve hemoglobin stability, several classes of compounds (see 5 Appendix A) were evaluated as additives to the base buffer formulation (also referred to herein as the existing buffer formulation) described above. These compounds were tested at various concentrations and in combination. A list of 10 the compounds tested using the accelerated shipping simulation and the results of these assays are found in Appendix B. A list of the compounds tested using the 3-day shipping simulation and their results are found in Appendix C. A list of the combinations of compounds that were tested is shown in Appendix D. Of the compounds evaluated, several were identified that show potential in enhancing 15 the stability of hemoglobin as additives to the existing buffer formulation. These compounds are listed in the table below: Compound Classification Compound Suga rs / Saccha rides Trehalose Sucrose Polygalacturonic Acid (Pectic Acid) Osmolytes / Extremolytes Betaine Trimethylamine N-oxide Polyvalent lons / Metal Salts Magnesium Sulfate Calcium Chloride HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) HRP Conjugate Stabilizer (PN SZ02) HRP Conjugate Stabilizer (PN ab!71537) 20          The compounds demonstrating the most potential for enhancing Hb stability from the accelerated shipping and other simulations were tested on three stool samples in several different shipping simulations. These results are shown below. In a 3-Day (72 hour) shipping simulation the potential additives demonstrate improved stability over the existing formulation (Hb Collection Buffer). See the following 25 table: 3-Day Shipping Simulation 2026214068   07 Aug 2026 Compound Classification Additive Additive Concentration % Hb Recovery Notes Hb Collection Buffer NA 75 Current formulation Polymedco (Eiken) Buffer NA 81 Osmolytes Betaine 3.5M 78 Improved Hb Stability Suga rs / Saccha rides Sucrose 0.24 M 75 Improved Hb Stability Suga rs / Saccha rides Trehalose 0.3 M 77 Improved Hb Stability Polyvalent lons / Metal Salts Calcium Chloride 10 mM 77 Improved Hb Stability Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 79 Improved Hb Stability HRP Stabilization Components HRP Conjugate Stabilizer (PN 85R-102) 15% 77 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 15% + 10 mM 97 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN 85R-102) + MgSO4 15% + 10 mM 87 Improved Hb Stability HRP Stabilization Components HRP Conjugate Stabilizer (PNSZ02) 15% 76 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN SZ02) + CaCI2 15% + 10 mM 88 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN SZ02) + MgSO4 15% + 10 mM 92 Improved Hb Stability The HRP (horse radish peroxidase) stabilizers tested and shown in the tables are: HRP Conjugate Stabilizer (PN 85R-102), Fitzgerald Industries International, 30 5 Sudbury Road, Suite 1A North, Acton, MA, 01720 USA HRP Conjugate Stabilizer (PN SZ02), Surmodics, Inc., 9924 West 74th Street, Eden Prairie, MN 55344 USA HRP Conjugate Stabilizer (PN abl71537), Abeam, 1 Kendall Square, Cambridge, MA 02139 USA 0 In a 7-Day shipping simulation the potential additives demonstrate improved stability over the existing formulation (Hb Collection Buffer). See the following Table: 2026214068   07 Aug 2026 7-Day Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Day 3 %Hb Recovery Day 5 % Hb Recovery Day 7 Notes Hb Collection Buffer NA 71 68 63 Current formulation Polymedco Buffer NA 83 83 80 Polyvalent lons / Metal Salts Calcium Chloride 10 mM 77 75 73 Improved Hb Stability Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 76 74 71 Improved Hb Stability Osmolytes Betaine 3.5M 78 74 70 Improved Hb Stability HRP Stabilization Components HRP Conjugate Stabilizer (PN 85R-102) 15% 80 75 69 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 15% + 10 mM 89 88 82 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN 85R-102) + MgSO4 15% + 10 mM 89 88 82 Improved Hb Stability HRP Stabilization Components HRP Conjugate Stabilizer (PN SZ02) 15% 80 70 61 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN SZ02) + CaCI2 15% + 10 mM 86 83 78 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN SZ02) + MgSO4 15% + 10 mM 90 85 79 Improved Hb Stability In a 3-Day extreme temperature shipping simulation the potential additives demonstrate improved stability over the existing formulation (Hb Collection Buffer). Additives containing HRP stabilization buffer and Calcium Chloride or Magnesium Sulfate 5 also demonstrate improved Hb stability compared to the Polymedco buffer. See following Table: 3-Day Extreme Temperature Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Notes Hb Collection Buffer NA NA 47 Current formulation Polymedco Buffer NA NA 67 Polyvalent lons / Metal Salts Calcium Chloride 10 mM 58 Improved Hb Stability Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 54 Improved Hb Stability 2026214068   07 Aug 2026 Osmolytes Betaine 3.5M 61 Improved Hb Stability HRP Stabilization Components HRP Conjugate Stabilizer (PN 85R-102) 15% 56 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 15% and 10 mM 77 Improved Hb Stability HRP Stabilization Components + Polyvalent Ions HRP Conjugate Stabilizer (PN 85R-102) + MgSO4 15% and 10 mM 71 Improved Hb Stability In an accelerated shipping simulation, the additive polygalacturonic acid (with calcium chloride) is demonstrating improved Hb stability over the existing formulation (Hb Collection Buffer) at different concentrations. See the following table: 5 Accelerated Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Notes Hb Collection Buffer NA NA 72 Current formulation Suga rs / Saccha rides Polygalacturonic Acid + Calcium Chloride 0.01%+ 10 mM 83 Improved Hb Stability Polygalacturonic Acid + Calcium Chloride 0.025%+ 10 mM 91 Improved Hb Stability Polygalacturonic Acid + Calcium Chloride 0.05%+ 10 mM 86 Improved Hb Stability Polygalacturonic Acid + Calcium Chloride 0.075%+ 10 mM 79 Improved Hb Stability Polygalacturonic Acid + Calcium Chloride 0.1%+ 10 mM 64 No Improvement Polygalacturonic Acid + Calcium Chloride 0.125%+ 10 mM 86 Improved Hb Stability 2026214068   07 Aug 2026 Another class of compounds called protoporphyrins was tested and can enhance the stability of hemoglobin when added to the existing buffer formulation. The protoporphyrins tested are shown in the table below. Note that in some test cases the pH was altered as solubility of protoporphyrins is known to be sensitive to pH. Table 14. Additional Potential Compounds Compound Classification Compound Protoporphyrin IX Zinc Protoporphyrin IX Cobalt Protoporphyrins Protoporphyrin IX Chromium Hemin (Protoporphyrin IX Iron) Hematin (Protoporphyrin IX Iron) In a 3-Day (72 hour) shipping simulation the existing formulation with added 10 protoporphyrin provided improved stability over the existing formulation without any protoporphyrin). These results are shown in the table below. 3-Day Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Notes Hb Collection Buffer NA 70 Current formulation Polymedco (Eiken) Buffer NA 80 Protoporphyrin Hemin (Buffer pH 6.5) 0.0003% (4.8 pM) 88 Improved Hb Stability Protoporphyrin Hemin (Buffer pH 7.4) 0.0003% (4.8 pM) 78 Improved Hb Stability Protoporphyrin Protoporphyrin IX Zn (Buffer pH 6.5) 1 pM 89 Improved Hb Stability Protoporphyrin Protoporphyrin IX Zn (Buffer pH 7.4) 1 pM 75 Improved Hb Stability Protoporphyrin Protoporphyrin IX Cr (Buffer pH 6.5) 2.5 pM 117 Improved Hb Stability Protoporphyrin Protoporphyrin IX Cr (Buffer pH 7.4) 2.5 pM 102 Improved Hb Stability Protoporphyrin Protoporphyrin IX Co (Buffer pH 6.5) 5 pM 109 Improved Hb Stability Protoporphyrin Protoporphyrin IX Co (Buffer pH 7.4) 5 pM 111 Improved Hb Stability In a 7-day shipping simulation the existing formulation with added protoporphyrin 15 provided improved stability over the existing formulation without any protoporphyrin. These results are shown in the table below. 2026214068   07 Aug 2026 7-Day Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Day 3 % Hb Recovery Day 5 % Hb Recovery Day 7 Notes Hb Collection Buffer NA 70 64 60 Current formulation Polymedco (Eiken) Buffer NA 83 82 80 Protoporphyrin Hemin (Buffer pH 6.5) 0.0003% (4.8 pM) 85 81 80 Improved Hb Stability Protoporphyrin Hemin (Buffer pH 7.4) 0.0003% (4.8 pM) 78 77 68 Improved Hb Stability Protoporphyrin Protoporphyrin IX Zn (Buffer pH 6.5) 1 pM 84 83 80 Improved Hb Stability Protoporphyrin Protoporphyrin IX Zn (Buffer pH 7.4) 1 pM 73 70 65 Improved Hb Stability Protoporphyrin Protoporphyrin IX Cr (Buffer pH 6.5) 2.5 pM 109 113 110 Improved Hb Stability Protoporphyrin Protoporphyrin IX Cr (Buffer pH 7.4) 2.5 pM 93 91 90 Improved Hb Stability Protoporphyrin Protoporphyrin IX Co (Buffer pH 6.5) 5 pM 101 104 104 Improved Hb Stability Protoporphyrin Protoporphyrin IX Co (Buffer pH 7.4) 5 pM 103 99 93 Improved Hb Stability Adding protoporphyrins to the existing formulation (Hb Collection Buffer) also 5 improves Hb stability in 3-Day extreme temperature shipping simulation. In addition, additives containing protoporphyrin cobalt (Co) or protoporphyrin chromium (Cr) demonstrate improved Hb stability compared to the Polymedco buffer. See the table below: 3-Day Extreme Temperature Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Notes Hb Collection Buffer NA NA 46 Current formulation Polymedco Buffer NA NA 69 Protoporphyrin Hemin (Buffer pH 6.5) 0.0003% (4.8 pM) 67 Improved Hb Stability Protoporphyrin Hemin (Buffer pH 7.4) 0.0003% (4.8 pM) 54 Improved Hb Stability Protoporphyrin Protoporphyrin IX Zn (Buffer pH 6.5) 1 pM 64 Improved Hb Stability 2026214068   07 Aug 2026 3-Day Extreme Temperature Shipping Simulation Compound Classification Additive Additive Concentration % Hb Recovery Notes Protoporphyrin Protoporphyrin IX Cr (Buffer pH 6.5) 2.5 pM 92 Improved Hb Stability Protoporphyrin Protoporphyrin IX Cr (Buffer pH 7.4) 2.5 pM 68 Improved Hb Stability Protoporphyrin Protoporphyrin IX Co (Buffer pH 6.5) 5 pM 81 Improved Hb Stability Protoporphyrin Protoporphyrin IX Co (Buffer pH 7.4) 5 pM 73 Improved Hb Stability FURTHER TESTING OF PROTOPORPHYRIN Protoporphyrin IX was tested at various concentrations and pHs using the 7-Day 5 Modified Shipping Simulation conditions listed below as well as other conditions. 7-Day Modified Shipping Simulation Simulated Shipping Condition Duration (hrs) Total Time (hrs) Temp (°C) Pre-shipping hold at ambient temperature 72 72 22 Pre-shipping temperature spike 8 80 35 Pre-shipping hold at ambient temperature 16 96 22 Ambient, overnight shipping 24 120 22 9 129 22 3 132 35 4 136 22 5 141 35 5 146 22 4 150 35 Post-shipping hold at ambient temperature 18 168 22 2026214068   07 Aug 2026 The following table shows that buffers containing Cobalt(III) PPIX (Protoporphyrin IX) at concentrations of 3-6 pM at an adjusted pH range of 6.4-7.0 provide increased Hb stability in the 7-Day Modified Shipping Simulation. 7-Day Modified Shipping Simulation Compound Classification Additive Additive Cone. Buffer pH % Hb Recovery Notes Hb Collection Buffer NA NA 7.4 59.4 Current Formulation Polymedco Buffer (Eiken) NA NA NA 70 Protoporphyrins Protoporphyrin IX Co 3 pM 6.9 75.0 Improved Hb Stability Protoporphyrin IX Co 3.5 pM 6.9 82.2 Improved Hb Stability Protoporphyrin IX Co 4 pM 6.4 100.0 Improved Hb Stability Protoporphyrin IX Co 4 pM 6.6 94.8 Improved Hb Stability Protoporphyrin IX Co 4 pM 6.8 93.3 Improved Hb Stability Protoporphyrin IX Co 4 pM 6.9 84.8 Improved Hb Stability Protoporphyrin IX Co 4 pM 7.0 87.7 Improved Hb Stability Protoporphyrin IX Co 4.5 pM 6.9 84.6 Improved Hb Stability Protoporphyrin IX Co 5 pM 6.4 105.0 Improved Hb Stability Protoporphyrin IX Co 5 pM 6.6 102.0 Improved Hb Stability Protoporphyrin IX Co 5 pM 6.8 95.5 Improved Hb Stability Protoporphyrin IX Co 5 pM 6.9 84.1 Improved Hb Stability Protoporphyrin IX Co 5 pM 7.0 95.7 Improved Hb Stability Protoporphyrin IX Co 6 pM 6.4 110.0 Improved Hb Stability Protoporphyrin IX Co 6 pM 6.6 106.0 Improved Hb Stability Protoporphyrin IX Co 6 pM 6.8 101.0 Improved Hb Stability Protoporphyrin IX Co 6 pM 7.0 95.0 Improved Hb Stability 2026214068   07 Aug 2026 The following table shows that buffers containing Cobalt(III) PPIX (Protoporphyrin IX) at concentrations of 2.5-10 pM at an adjusted pH of 6.8 provide increased Hb stability in the 3 and 7 day Shipping Simulations. The following table shows that buffers containing Chromium(III) PPIX 5 (Protoporphyrin IX) at concentrations of 0.5-10 pM at an adjusted pH of 7.1 provide increased Hb stability in the 3 and 7 day Shipping Simulations. 3-Dayand 7-Day Shipping Simulation Compound Class Additive Additive Cone. Buffer PH 3-Day % Hb Recovery 7-Day % Hb Recovery Notes Hb Collection Buffer NA NA 7.4 61.5 54.8 Current Formulation Protoporphyrin Protoporphyrin IX Co 2.5 pM 6.8 80.2 79.5 Improved Hb Stability 3 pM 6.8 76.0 73.4 Improved Hb Stability 5 pM 6.8 82.8 82.7 Improved Hb Stability 7 pM 6.8 86.5 83.7 Improved Hb Stability 7.5 pM 6.8 92.9 94.8 Improved Hb Stability 10 pM 6.8 95.5 101.3 Improved Hb Stability Protoporphyrin IX Cr 0.5 pM 7.1 75.0 65.6 Improved Hb Stability 2.5 pM 7.1 86.3 78.9 Improved Hb Stability 4.5 pM 7.1 94.5 86.4 Improved Hb Stability 5 pM 7.1 97.0 99.4 Improved Hb Stability 7.5 pM 7.1 108.7 112.7 Improved Hb Stability 2026214068   07 Aug 2026 10 pM 7.1 116.8 113.8 Improved Hb Stability The following table shows that buffers containing Cobalt(III) PPIX (Protoporphyrin IX) at concentrations of 2.5-10 pM at an adjusted pH range of 6.2-7.4 provide increased Hb stability in the 35C 16 hour challenge. 5          The following table shows that buffers containing Chromium(III) PPIX (Protoporphyrin IX) at concentrations of 1.25-5 pM at an adjusted pH range of 6.2-7.4 provide increased Hb stability in the 35C 16 hour challenge. 35C 16 hour challenge Compound Class. Additive Additive Cone. Buffer PH %Recovery Notes Hb Collection Buffer NA NA 7.4 71.2 Current Formulation Protoporphyrin Protoporphyrin IX Co 2.5 pM 6.2 76.0 Improved Hb Stability 2.5 pM 6.5 84.1 Improved Hb Stability 2.5 pM 6.8 82.3 Improved Hb Stability 2.5 pM 7.1 80.4 Improved Hb Stability 5.0 pM 6.2 104.3 Improved Hb Stability 5.0 pM 6.5 105.5 Improved Hb Stability 5.0 pM 6.8 93.8 Improved Hb Stability 5.0 pM 7.1 85.1 Improved Hb Stability 5.0 pM 7.4 78.4 Improved Hb Stability 10 pM 6.2 116.0 Improved Hb Stability 10 pM 6.8 107.6 Improved Hb Stability 10 pM 7.1 112.9 Improved Hb Stability 10 pM 7.4 98.5 Improved Hb Stability Protoporphyrin Protoporphyrin IX Cr 1.25 pM 6.2 74.0 Improved Hb Stability 1.25 pM 6.5 77.0 Improved Hb Stability 2026214068   07 Aug 2026 1.25 pM 6.8 105.0 Improved Hb Stability 1.25 pM 7.1 77.5 Improved Hb Stability 1.25 pM 7.4 73.5 Improved Hb Stability 2.5 pM 6.2 79.8 Improved Hb Stability 2.5 pM 6.5 86.3 Improved Hb Stability 2.5 pM 6.8 85.0 Improved Hb Stability 2.5 pM 7.1 81.8 Improved Hb Stability 2.5 pM 7.4 73.1 Improved Hb Stability 5 pM 6.2 87.5 Improved Hb Stability 5 pM 6.5 89.6 Improved Hb Stability 5 pM 7.1 86.0 Improved Hb Stability 5 pM 7.4 75.5 Improved Hb Stability Accordingly, the preceding merely illustrates the principles of the invention. It will be appreciated that those skilled in the art will be able to devise various arrangements which, 5 although not explicitly described or shown herein, embody the principles of the invention and are included within its spirit and scope. Furthermore, all examples and conditional language recited herein are principally intended to aid the reader in understanding the principles of the invention and the concepts contributed by the inventors to furthering the art and are to be construed as being without limitation to such specifically recited examples and conditions. 10 Moreover, all statements herein reciting principles, aspects, and embodiments of the invention as well as specific examples thereof, are intended to encompass both structural and functional equivalents thereof. Additionally, it is intended that such equivalents include both currently known equivalents and equivalents developed in the future, i.e., any elements developed that perform the same function, regardless of structure. The scope of the present 15 invention, therefore, is not intended to be limited to the exemplary embodiments shown and described herein. Rather, the scope and spirit of the present invention is embodied by the appended claims. 2026214068   07 Aug 2026 By way of clarification and for avoidance of doubt, as used herein and except where the context requires otherwise, the term "comprise" and variations of the term, such as "comprising", "comprises" and "comprised", are not intended to exclude further additions, components, integers or steps. 5 Reference to any prior art in the specification is not an acknowledgement or suggestion that this prior art forms part of the common general knowledge in any jurisdiction or that this prior art could reasonably be expected to be combined with any other piece of prior art by a skilled person in the art. 1005261688 2026214068   07 Aug 2026 Compound Classification Metal Chelators Flavoniods Protease / Phosphatase Inhibitors Crosslinkers Similar to Drabkin's Reagent (cyanide) Non-covalent Hb interactions Sulfonic Acids Preservatives with thiol groups ___________________Compound___________________ ______________________Nitrilotriacetic acid (NTA)______________________ __________Ethy!enediamine-N,N'-disuccinic add (EDDS)__________ __________diethylenetriaminepentaacetic acid (DTPA)__________ ___________________Iminodisuccinic acid (IDS; ISA)___________________ N,N-bis-(Carboxymethyl)-L-glutamic acid tetrasodiumn salt (GLDA) Trisodium N-(1-Carboxylatoethyl)iminodiacetate Hydrate (MGDA) Phytic Acid (IP6) ATMP - Amino tris (methylene phosphonic acid) Iminodiacetic Acid (IDA) sodium tripolyphosphate _________ethylenediamine-N,N,N',N'-4-acetic acid (EDTA)_________ ____________________________quercetin___________________________ _________________________chrysin- pyridine_________________________ ______________________daidzein - DMSO______________________ _____________________7,8-Dihydroxyflavone_____________________ aurintircarboxylic acid ammonium salt _____________________methylisothiazolone_____________________ ____________________________Proci in 300____________________________ _______________________sodium fluoride_______________________ ___________________________poly(acrylic acid)___________________________ _________________4arm-PEG20K-Maleimide_________________ ___________________mPEG-Maleimide, 5K___________________ glutaraldehyde Dimethyl 3,3'-dithiopropionimidate dihydrochloride bis(3,5-dibromosalicyl) fumarate formaldehyde solution __________________magnesium gluconate__________________ ________________________cysteamine________________________ methylglyoxal Imidazole hydroxylamine Histidine polysulfonated polymers (dextran sulphate) heparin _________________PEG 2000_________________ ________________PEG 20000________________ ________________PEG 8000________________ ___________Bovine Serum Albumin___________ _________________Poly vinyl alcohol_________________ methanedisulfonic acid dipotassium salt ______propanedisulfonic acid disodium salt______ piperazine-N,N'-bis (2-ethanesulfonic acid) (PIPES) ______________Sodium Thiosulfate______________ ____________________Cysteine____________________ glutathione 2026214068   07 Aug 2026 Sugars / Saccharides dithiothreitol TCEP trehalose Glucose ___________raffinose__________ _________corn starch_________ ________potato starch________ _______Gum arable_______ _______xanthan gum_______ ___________Lactose__________ _________Maltose_________ __________Fructose__________ _________Galactose_________ _______Isomaltotriose_______ _________Maltotriose________ Melezitose Kestose Stachyose Glucose Tetrasaccharide Erlose ________Mannose________ Polygalacturonic Acid Galacturonic Acid (monomer) Sodium Alginate (synthetic) Redox / antioxidants Formaldehyde Releasers ____________Sodium Alginate (Medium Viscosity)____________ _______________Sodium Alginate (Low Viscosity)_______________ ______________________________lipoic acid______________________________ ___________________mPEG2K-Thioctic acid__________________ _________________________ascorbic acid_________________________ ________________________glutathione________________________ ________________Deferoxamine mesylate salt________________ uric acid urea Imidazolidinyl Urea Bronopol Diazolidinyl Urea _________________DMDM Hydantoin_________________ _____________Sodium hydroxymethyl glycinate_____________ _____________Tris(Hydroxymethyl)nitromethane_____________ _______________5-bromo-5-nitro-l,3-dioxane_______________ _____________2-bromo-2-nitro-l,3-propanediol_____________ l,3-Bis(hydroxymethyl)-5,5-dimethylimidazolidine-2,4-dione Streck Cell Preservative Solution _______________________Styrene Glycol_______________________ ______________________Styrene Oxide______________________ Sodium Bicarbonate 2026214068   07 Aug 2026 Substrates DPG, diphospho glycerate Arginine Lysine Resveratrol Protoporphyrin IX Zn Protoporphyrins Osmolytes / Extremoiytes Formaldehyde + Ammonium Complexes Salt ______Protoporphyrin IX______ Protoporphyrin IX Cobalt Protoporphyrin IX Chromium Protoporphyrin IX Magnesium Protoporphyrin IX Manganese Protoporphyrin IX Tin Protoporphyrin IX Gallium _______Hemin, Bovine_______ _________Hematin_________ Bilirubin ___________Biliverdin___________ ___________Betaine___________ Trimethylamine N-oxide ___________Ectoine___________ Firoin paraformaldehyde_____ formaldehyde solution ammonium chloride ammonium sulfate ammonium acetate ammonium oxalate hexamethylenetetramine Ionic Liquids _______l-Allyl-3-methylimidazolium chloride_______ ______l-Butyl-3-methylimidazolium chloride______ l-butyl-2,3-dimethylimidazolium tetrafluoroborate l-butyl-3-methylimidazoliumtetrafluoroborate l-Ethyl-3-methylimidazolium chloride 2-(Diethylamino)ethanol Methyl hexanoate 1 -hexyl-3-methylimidazolium chloride 1 -decyl-3-methylimidazolium chloride Sodium Bromide Choline Chloride Deep Eutectic Solvents Tetrabutylammonium bromide urea Imidazole Glycerol Ethylene Glycol D-glucose Fe (III) Chloride 2026214068   07 Aug 2026 Polyvalent lons / Metal Salts HRP Stabilizer Components Vitamins Cobalt Nickel Copper Zinc _________________Sodium Bisulfite_________________ ______________Sodium Carbonate______________ _______________Sodium Perchlorate_______________ ______________Sodium Phosphate______________ ________________Sodium Acetate________________ _______________Potassium Chloride_______________ ______________Magnesium Chloride______________ _______________Potassium Acetate_______________ _________________Lithium Chloride_________________ ______________________Iron (II) Sulfate_____________________ ______________Magnesium Sulfate______________ ____________________Zinc chloride____________________ Calcium Chloride Calcium Gluconate Calcium Propoinate Calcium Propoinate 8-anilino-l-napthalene sulfonic acid ammonium salt _________aluminum chloride hexahydrate_________ _______________4-bromophenol_______________ 2-methyl-4-isothiazolin-3-one-hydrochloride ___________5-bromo-5-nitro-l,3-dioxane___________ _______AAT Bioquest HRP Stabilization Buffer_______ _________________Azobenzene_________________ __________________Phenol Red__________________ ____________________Proclin 300____________________ __________Candor HRP Stabilization Buffer__________ __________Innova HRP Stabilization Buffer__________ ________Surmodics HRP Stabilization Buffer________ BioRad HRP Stabilization Buffer Sigma HRP Stabilization Buffer Guardian Peroxidase Conjugate Buffer Fitzgerald HRP Conjugate Buffer ___________Sigma HRP Stabilization Buffer__________ ________Pyridoxal S'-phosphate hydrate (B6)________ ................................................................................................................NAD...........................................................................................................~ _________________Vitamin B12_________________ ________________NADH________________ _________________Nicotinic Acid (B3)_________________ ____________________Folic Acid (M)____________________ ________________Nicotinamide (B3)________________ ___________Vitamin E (alpha tocopherol)___________ _________________Sodium Nitrate_________________ Sodium Nitrite 2026214068   07 Aug 2026 Preservatives / Parabens Aldehydes Plasma Gelatins Detergents Solvents Sodium Citrate Sodium Benzoate Ethyl 4-hydroxybenzoate Methyl 4-hydroxybenzoate _______Propyl 4-hydroxybenzoate_______ _______Benzyl 4-hydroxybenzoate_______ _________formaldehyde solution_________ __________paraformaldehyde__________ ____________Glutaraldehyde____________ ___________________Furfural__________________ ____________p-Tolualdehyde____________ __________Cinnamaldehyde__________ _____________Butyraldehyde____________ _________Glycolaldehyde Dimer_________ ____________Glyceraldehyde____________ ____________Formamide____________ 4-lsobutylbenzaldehyde Benzaldehyde Acetylaldehyde Vanillin ___________Human Plasma___________ ______________Albumin______________ _________________Glycine_________________ ___________________HDL.___________________ _________________LDL_________________ __________Phosphatidylcholine__________ ____________Cohns Fraction ll / lll____________ ____________Cohns Fraction IV____________ __________Fetal Bovine Serum__________ ____________Bovine Plasma____________ __________Gamma Globulins__________ Gelatin - Bovine Type B (225 g bloom) Gelatin - Porcine Type A (90-110 g bloom) Gelatin - Porcine Type A (175 g bloom) Gelatin - Porcine Type A (300 g bloom) Gelatin - Cold Water Fish Skin _____________________Brij 35____________________ _____________Tween 20_____________ .....................................................................TritonxI66..................................................... ________________IN HCI________________ ___________IM NaOH___________ _____________IM Tris, pH 7.5_____________ pyridine DMSO 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts None Hb Collection Buffer - No additive NA 68 Current Buffer Formulation None Polymedco Buffer - No additive NA 80 ionic Liquids l-ethyl-3-methylimidazolium chloride 1 rnM 68 No Improvement ionic Liquids l-ethyl-3-methylimidazoiium chloride 10 mM 69 No improvement ionic Liquids l-ethyl-3-methylimidazolium chloride 100 mM 65 No improvement Ionic Liquids 1- h exv 1 - 3- m ethv 1 i m id a z o 1 i u m c h i o ri d e 0.02% 67 No Improvement ionic Liquids 1-hexyl-3-methylimidazolium chloride 0.20% 61 No Improvement ionic Liquids l-hexyl-3-methylimidazolium chloride 1% 42 No Improvement Protoporphyrin Protoprophyrin IX 0.001 mM 65 No Improvement Protoporphyrin Protoprophyrin IX 0.01 mM 62 No improvement Protoporphyrin Protoprophyrin IX O.i" rnM 63 No improvement Protoporphyrin Protoprophyrin IX Zn 0.001 mM 68 No Improvement Protoporphyrin Protoprophyrin IX Zn 0.01 mM 59 No Improvement Protoporphyrin Protoprophyrin IX Zn 0.1 mM 53 No Improvement Formaldehyde Releaser Sodium hydroxyimethyi glycinate 0.1 mM 70 No improvement Formaldehyde Releaser Sodium hydroxylmethyl glycinate 1 mM 64 No improvement Formaldehyde Reieaser Sodium hydroxylmethyl glycinate 10 mM 66 No Improvement Osmolytes Trimethyiamine N-oxide (TMANO) 50 mM 76 No Improvement Osmolytes Trimethyiamine N-oxide (TMANO) 100 mM 72 No Improvement Osmolytes Trimethyiamine N-oxide (TMANO) 200 mM 74 No improvement Protoporphyrin Protoprophyrin IX + FeCi3 0.1 mM + 0.1 mM 62 No improvement Protoporphyrin Protoprophyrin IX + FeCI3 1mM + ImM 53 No Improvement Protoporphyrin Protoprophyrin IX + FeCI3 10 mM + 10 mM 40 No Improvement Crosslinkers Poly Acrylic Acid 1 rnM 64 No Improvement Crosslinkers Poly Acrylic Acid 5 mM 56 No Improvement Crosslinkers Poly Acrylic Acid 7 mM 58 No improvement Crosslinkers 4arm-PF.G2K-Maieimide + Iminothioiane hydrochloride 0.062 rnM + 1 mM 67 No Improvement Crosslinkers 4arm-PEG2K-Maleimide + Iminothioiane hydrochloride 0.125 mM + 2 mM 66 No Improvement Crosslinkers 4arm-PEG2K-Maleimide +• Iminothioiane hydrochloride 2.5 mM + 10 mM 27 No Improvement Crosslinkers mPhG-Mal, hK + Iminothioiane hydrochloride 0.5 mM + 1 mM 65 No Improvement Crosslinkers mPEG-Mal, 5K + Iminothioiane hydrochloride 1mM + 2mM 58 No improvement Crosslinkers mPEG-Mal, 5K + Iminothioiane hydrochloride 5 mM + 10 mM 56 No improvement Plasma Human Plasma, lyopholyzed 1% 97 High Background Signal Plasma Human Plasma, lyopholyzed 5% 98 High Background Signal Plasma Human Plasma, lyopholyzed 10% 97 High Background Signal Plasma Human Plasma, lyopholyzed 25% 94 High Background Signal Plasma Human Plasma, lyopholyzed 50% 95 High Background Signal Plasma Human Plasma, lyopholyzed 1% 96 High Background Signa! Plasma Human Plasma, lyopholyzed 5% 97 High Background Signal Plasma Human Plasma, lyopholyzed 10% 96 High Background Signal Plasma Human Plasma, lyopholyzed 25% 98 High Background Signal Plasma Human Plasma, lyopholyzed 50% 99 High Background Signa! 2026214068   07 Aug 2026 Compound Adddivs Additive CatxeAimitm dee&very Resufts Pi^srYia Human Piasma, lyopholyzed 1% y / High. Background Signa: Plasma Human Piasma, lyopholyzed 0,50% 92 High Background Signal Piasma Human Plasma, lyopholyzed 0.10% 99 High Background Signal Plasma Human Plasma, lyopholyzed 0.05% 161’ High Background Signal Piasma Human Plasma, lyopholyzed 0.01% 81 High Background Signal Piasma Human Plasma, lyopholyzed 0.005% 81 High Background Signal Piasma Human Piasma, lyopholyzed o.ooi% 71 High Background Signal Piasma Human Plasma, lyopholyzed 0.005% 77 High Background Signal Piasma Human Plasma, lyopholyzed 0.01% 80 High Background Signal Piasma Human Plasma, lyopholyzed 0.02% 88 High Background Signal Piasma Human Plasma, lyopholyzed 0.03% 93 High Background Signal Piasma Human Plasma, lyopholyzed 0.04% 97 High Background Signa! Piasma Human Plasma, lyopholyzed 0.05% 95 High Background Signal Piasma Human Plasma, lyopholyzed 0.10% 99 High Background Signal Piasma Albumin, Human 0 0025 mg / mL 71 No Improvement Piasma Albumin, Human 0.025 mg / mL 90 No Improvement Piasma Albumin, Human 0.25 mg / mL 98 No Improvement Piasma Albumin, Human 2,5 mg / mL 99 No Improvement Piasma Albumin, Human 25 mg / mL 98 No Improvement Piasma Glycine 0.0002 mg / mL 68 No Improvement Piasma Glycine 0.002 mg / mL 68 No Improvement Piasma Glycine 0.02 mg / mL 71 No Improvement Piasma Cohn's Fraction ii / iii 0.005 mg / mL 70 High Background Signal Piasma Cohn's Fraction ll / lil 0.05 mg / mL 80 High Background Signal Piasma Cohn's Fraction ll / lil 0.5 mg / mL 99 High Background Signal Piasma Cohn's Fraction II / III 5 mg / mL 100 High Background Signal Piasma Cohn’s Fraction II / III 50 mg / mL 97 High Background Signal Piasma Cohn's Fraction IV 0.005 mg / mL 106 High Background Signa! Piasma Cohn's Fraction IV 0.05 mg / mL 100 High Background Signal Piasma Cohn's Fraction IV 0.5 mg / mL 98 High Background Signal Piasma Cohn's Fraction IV 5 mg / mL 96 High Background Signal Piasma Cohn's Fraction IV 50 mg / mL 98 High Background Signa! Piasma HDL 0.0025 mg / mL 69 No Improvement Piasma HDL 0.025 mg / mL 69 No Improvement Piasma HDL 0.25 mg / mL 70 No Improvement Piasma LDL 0.0025 mg / mL 69 No Improvement Piasma LDL 0.025 mg / mL 70 No Improvement Piasma LDL 0.25 mg / mL 67 No Improvement Piasma p h os p h a t i d y 1 c h o I i n e 0.01 mg / mL 67 No Improvement Piasma — phosphatidylcholine 0.1 mg / mL 66 No Improvement phLCpudliiJylLluliiie 1 mg / mL 62 No Improvement Poiyvaient lons / Metal Saits Calcium Chloride 1 mM 74 UaTcTum cation sfiows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride 10 mM 78 calcium cation snows improvement in %Hb Recovery at 10 mM Poiyvaient lons / Metal Salts Calcium Chloride 100 mM 119 caicium cation snows improvement in %Hb Recovery at 10 rnM Deep Eutectic Solvents Ethylene Glycol 5% 69 No Improvement Deep Eutectic Solvents Ethylene Glycol 10% 63 No Improvement Deep Eutectic Solvents Ethylene Glycol 20% 54 No Improvement Polyvalent lons / Metal Salts Ethylene Glycol + Calcium Chloride 10% + 10 mM 70 No Improvement Polyvalent lons / Metal Salts Ethylene Glycol + Calcium Chloride 2.5%+ 2.5 mM 70 No Improvement Polyvalent lons / Metal Salts Ethylene Glycol + Calcium Chloride 5% + 5 mM 72 No Improvement Non-covalent Hb interactions Dextran Sulphate 3.75% 67 No Improvement 2026214068   07 Aug 2026 Compound Additive Additive CotxsAtmton Recovery R&sufts Non-covaiem Hb interactions Dextran Sulphate ! .5U% 63 No Improvement Non-covaient Hb interactions Dextran Sulphate 15% 70 No Improvement Osmoiytes Betaine 125 mM 72 Betaine shows improvement in %Hb Recovery at high concentrations Osmoiytes Betaine 250 mM 76 Betaine shows improvement in %Hb Recovery at high concentrations Osmoiytes Betaine 500 mM 78 Betaine shows improvement in %Hb Recovery at high concentrations Suga rs / Saccha rides Trehalose + Betaine 125 mM + 62.5 mM 77 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Trehalose + Betaine 250 mM F 125 mM 78 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Trehalose + Betaine 500 mM + 250 mM 79 Slight improvement wifh Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose + Betaine 250 mM + 62.5 mM 72 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Glucose + Betaine 500 mM + 125 mM 76 Slight Improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Glucose + Betaine 1 M + 250 mM 78 STighf i m pfovemehf wit ri Sugars. Needs further assay optimization Suga rs / Sa ccha rides Glucose + Glycerol 250 mM + 2.5%, 73 siignt improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Glucose + Glycerol 500 mM + 5% 71 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides -J Glucose + Glycerol 1 M + 10 % 73 SIighf improvemehf wifn Sugars. Needs further assay optimization Suga rs / Sa ccha rides Glucose r Glycine 250 mM + 62.5 mM 72 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose + Glycine 500 mM + 125 mM 78 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Glucose + Glycine 1 M + 250 mM 77 SligfftTrh pfbvemenf wit ri Sugars. Needs further assay optimization Suga rs / Sa ccha rides Trehalose + Glycerol 125 mM 2.5 % 71 Might improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Trehalose + Glycerol 250 mM + 5% 72 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Trehalose + Glycine 125 mM 62.5 mM 70 Slight improvement with' Sugars. Needs further assay optimization Suga rs / Sa ccha rides Trehalose + Glycine 250 mM t 125 mM 72 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Trehalose + Glycine 500 mM + 250 mM 79 Slight improvement with Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery R&sufts Sugars / Saccharides Trehalose 1 M 77 Slight improvement with Sugars. Needs further assay optimization Polyvalent lons / Metal Salts Calcium Chloride 5 mM 74 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride 10 mM 67 Calcium cation sHows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride 25 mM 75 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium: Chloride 50 mM 68 Caicium cation shows improvement in %Hb Recovery at 10 rnM Polyvalent lons / Metal Salts Calcium Chloride 100 mM 51 Caicium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + PEG 2000 0.075% + 1% 74 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium: Chloride + PEG 2000 0.15% + 2% 71 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + PEG 2000 0.3% + 4% 62 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride + PEG 20000 0.075% + 1% 74 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + PEG 20000 0.15%+ 2% 72 Caicium cation shows improvement in %Hb Recovery at 10 rnM Polyvalent lons / Metal Salts Calcium Chloride + PEG 20000 0.3% + 4% 66 Caicium cation snows improvement in %Hb Recovery at 10 mM Poiyvaient lons / Metal Salts Calcium Chloride + PEG 8000 0.075% + 1% Calcium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + PEG 8000 0.15%, + 2% 75 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + PEG 8000 0.3% + 4% 67 Calcium cation shows improvement in %Hb Recovery at 10 mM Formaldehyde + Ammonium Salt Complexes Ammonium Acetate 100 mM 58 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Acetate 25 mM 58 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium: Acetate 50 mM 59 No Improvement Formaldehyde + Ammonium Sait Complexes Ammonium Chloride 100 mM 58 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Chloride 25 mM 104 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Chloride 50 mM 58 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Oxalate 10 mM 62 No Improvement Formaldehyde <- Ammonium Salt Complexes Ammonium Oxalate 2.5 mM 60 No Improvement r-ormaidehyde + Ammonium Sait Complexes Ammonium Oxalate 5 mM 59 No Improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CotxsAtmton Recovery Resufts formaldehyde t Ammonium Salt Complexes Ammonium Sulfate 100 mM 61 No Improvement i-ormaidehyde + Ammonium Salt Complexes Ammonium Sulfate 25 mM 60 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Sulfate 50 mM 58 No Improvement Polyvalent lons / Metal Salts Calcium Gluconate 100 mM 57 No Improvement Polyvalent lons / Metal Salts Calcium Gluconate 25 mM 66 No Improvement Polyvalent lons / Metal Salts Calcium Gluconate 50 mM 65 No Improvement Sugars / Saccharides Glucose + Calcium Chloride 0.5 M + 0.5% 65 No Improvement Suga rs / Saccha rides Glucose + Calcium Chioride 1 M + 1% 65 No Improvement Sugars / Saccharides Glucose + Calcium Chloride 2 M + 2% 61 No Improvement Polyvalent lons / Metal Salts Magnesium Gluconate 100 mM 58 No Improvement Polyvalent lons / Metal Salts Magnesium Gluconate 25 mM 69 No Improvement Polyvalent lons / Metal Salts Magnesium Gluconate 50 mM 65 No Improvement Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) 0.1 mM 73 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) 1 mM 71 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) 2 mM 72 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) 4 mM 73 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride (anhydrous) 6 mM 77 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) S mM 75 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) 10 mM 79 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (anhydrous) 2.0 mM 76 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (dihydrate) 0.1 mM 71 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (dihydrate) 1 mM 70 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride (dihydrate) 2 mM 71 UaTcTum cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride (dihydrate) 4 mM 74 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (dihydrate) 6 mM 74 Caicium cation shows improvement in %Hb Recovery at 10 rnM Polyvalent lons / Metal Salts Calcium Chloride (dihydrate) 8 mM 72 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (dihydrate) 10 mM 77 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride (dihydrate) 20 mM 73 Calcium cation shows improvement in %Hb Recovery at 10 mM 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts Formakiehyde Reieaser 5- b ? o rn o- 5- n i bo-1.3 -dioxa n e 0.0002% 69 No Improvement Formaldehyde Releaser 5- brom o-5- n itro- 1,3-dioxa ne 0,001% 68 No Improvement Formaldehyde Reieaser 5-bromo-5-nitro-l,3-dioxane 0.002% 70 No Improvement Enzyme inhibitor 2-methyl-4-isothiazolin-3-one-hvdrochloride 0.0002% 71 No Improvement Enzyme Inhibitor 2-metnyl-4-isothiazohn-3-one-hydrochioride 0.001% 68 No Improvement Enzyme Inhibitor 2-methyl-4-isothiazolin-3-one-hydrochloride 0.002% 63 No Improvement HRP Stabilizer Components Phenol Red 0.01% 70 No Improvement HRP Stabilizer Components Phenol Red 0.03% 71 No Improvement HRP Stabilizer Components Phenol Red 0.05% 70 No Improvement Preservatives Sodium Benzoate 0.10% 67 No Improvement Preservatives Sodium Benzoate 0.50% 60 No Improvement Preservatives Sodium Benzoate 1% 54 No Improvement HRP Stabilizer Components Sigma HRP Stabilization Buffer 5% 71 No Improvement HRP Stabilizer Components Sigma HRP Stabilization Buffer 10% 70 No Improvement HRP Stabilizer Components Sigma HRP Stabilization Buffer 25% 68 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 5% Additive 83 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 5% Component 71 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Gluconate 100 mM 57 No Improvement Polyvalent lons / Metal Salts Calcium Gluconate 25 mM 66 No Improvement Polyvalent lons / Metal Salts Calcium Gluconate 50 mM 65 No Improvement Suga rs / Saccha rides Glucose + Calcium Chloride 0.5 M + 0.5% 65 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Glucose + Calcium Chloride 1 M + 1% 65 Slight improvement with' Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose + Calcium: Chloride 2 M + 2% 61 Slight improvement with Sugars. Needs further assay optimization Polyvalent lons / Metal Saits Magnesium Gluconate 100 mM 58 No Improvement Polyvalent lons / Metal Salts Magnesium Gluconate 25 mM 69 No Improvement Polyvalent lons / Metal Salts Magnesium Gluconate 50 mM 65 No Improvement Polyvalent lons / Metal Salts Iron (2) Chloride 36 mM 60 No improvement Polyvalent lons / Metal Salts Iron (2) Chloride 43.2 mM 51 No improvement Polyvalent lons / Metal Salts Iron (2) Chloride 48 mM 27 No Improvement Polyvalent lons / Metal Salts Lithium Chloride 100 mM 68 No Improvement Polyvalent lons / Metal Salts Lithium: Chloride 150 mM 66 No Improvement Polyvalent lons / Metal Salts Lithium Chloride 260 mM 66 No improvement Polyvalent lons / Metal Salts Magnesium Chloride 100 mM 62 Magnesium cation snows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Chloride 150 mM 61 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Saits Magnesium Chloride 192 mM 57 Magnesium Uation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Potassium Acetate 100 mM 65 No Improvement Polyvalent lons / Metal Salts Potassium Acetate 150 mM 69 No Improvement Polyvalent lons / Metal Salts Potassium Acetate 260 mM 67 No improvement Polyvalent lonsjMetal Salts Potassium: Chloride 100 mM 53 No improvement Polyvalent lons / Metal Saits Potassium Chloride 150 mM 63 No Improvement Polyvalent lons / Metal Salts Potassium Chloride 260 mM 66 No Improvement Polyvalent lons / Metal Salts Sodium Acetate 166 mM 55 No Improvement Polyvalent lons / Metal Salts Sodium Acetate 150 mM 40 No improvement Polyvalent lons / Metal Salts Sodium Acetate 260 mM 45 No improvement 2026214068   07 Aug 2026 Compound Additive Recovery Resufts buost rates Sodium Bicarbonate 100 mM 46 No Improvement Substrates Sodium Bicarbonate 150 mM 43 No Improvement Substrates Sodium Bicarbonate 260 mM 42 No Improvement Polyvalent lons / Metal Salts Sodium Bisulfite 101.055 mM 38 No Improvement Polyvalent lons / Metal Salts Sodium Bisulfite 75.791 mM 46 No Improvement Polyvalent lons / Metal Salts Sodium Bisulfite 90.95 mM 41 No Improvement Polyvalent lons / Metal Salts Sodium Bromide 100 mM 65 No Improvement Polyvalent lons / Metal Saits Sodium Bromide 150 mM 64 No Improvement Polyvalent lons / Metal Salts Sodium: Bromide 192 mM 59 No Improvement Polyvalent lons / Metal Salts Sodium Carbonate 38,207 mM 28 No Improvement Polyvalent lons / Metal Salts Sodium Carbonate 45.848 mM 24 No Improvement Polyvalent lons / Metal Salts Sodium Carbonate 50.942 mM 25 No Improvement Polyvalent lons / Metal Saits Sodium Perchlorate 100 mM 47 No Improvement Polyvalent lons / Metal Salts Sodium Perchlorate 150 mM 43 No Improvement Polyvalent lons / Metal Salts Sodium Perchlorate 260 mM 38 No Improvement Poiyvaient lons / Metal Salts Sodium Phosphate 100 mM 65 No Improvement Polyvalent lons / Metal Salts Sodium Phosphate 150 mM 65 No Improvement Polyvalent lons / Metal Saits Sodium Phosphate 260 mM 66 No Improvement HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 10% 78 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 11% 77 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 12% 78 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 13% 81 Slight Improvement, Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 14% 80 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 15% 81 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 16% 90 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 17% 81 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 18% 87 Slight Improvement, Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 19% 85 Slight Improvement, Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 20% 83 Siig'nt Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 21% 86 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 22% 86 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 23% 89 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 24% 86 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer 25% 84 Slight Improvement. Needs further assay optimization Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc Chloride 1% (50g / mL, 100 mL / L, 5.8 S / L) 34 No Improvement Formaldehyde Releaser Diazolidiny 1 Urea + DMSO s Zinc Chloride 5%. (50g / mL, 100 ml / L, 5,8 g / L) 62 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO t Zinc Chloride 10% (50g / mt, 100 ml / L, 5,8 g / l) 58 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc Chloride 2594 (50g / mL, 100 ml / L, 5.3 g / L) 62 No Improvement Formaldehyde Releaser Diazolidinyl Urea +• DMSO + Zinc Chloride 50% (50g / mL, 100 mL / L, 5.8 g / L) 30 No Improvement Plasma Bovine B J-g / L 68 No Improvement plasma Bovine B 2g / L 65 No Improvement 2026214068   07 Aug 2026 Polyvalent lons / Met Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent Ions / Me1 Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent Ions / Me1 Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Polyvalent lons / Mel Ch SJ Gelatins o CD in it / 3J Gelatins Gelatins CD 0J 2 o ru tu in suiieiag Gelatins o fp GU CD CD 3? Cu’ 3 f : § : i i & ■<<<^<<<<: □J 01 n; QJ QJ QJ £U uJ n; □J CD QJ £U uJ CD cd (ZJ & tn CD tZ> CD a? tn CD a? UT> CD CT in in CT CT w in in CT CT w in tn Magnesium Sulfate Magnesium Sulfate Magnesium Sulfate Magnesium Sulfate Magnesium Gluconate Magnesium Gluconate Magnesium Gluconate Magnesium Gluconate Magnesium Gluconate Magnesium Gluconate Magnesium Chloride Magnesium Chloride Magnesium Chloride Magnesium Chloride Magnesium Chloride Magnesium Chloride Procine A (300g) ir Gu o o O ro UJ o o V aupcjj Procine A (l / 5g) Procine A (175g) O > i--' o go. C ir o o 3 > do era. CW Fish Skin Q ^;>iS AAD co o ir co & : III 30 mM 20 mM 10 mM 5 mM 50 mM <40 mM 30 mM 20 mM 10 mM 5 mM 50 mM — 40 mM 30 mM 20 mM 10 mM 5 mM r" NJ r" CTO* CT 2g / L lg / L — go 2g / L / 7 4g / L M S3 r— do & 1 n i S i : 1 & Xi w di Ln rd -cd Ch J Ci cn Cm CD cn rd 2 00 UJ cn 00 2 co CU n o 2 aj 3 "□ 2 0) 2 Magnesium Recovery ar improverr lunisauSejAi Recovery ar XOAOJdUJi Magnesium Recovery ar Magnesium improverr Recovery ar improverr Magnesium Recovery ar improverr Magnesium Recovery ar improverr lumsauSBiAj Recovery ar improverr Magnesium Recovery ar XOAOJdUJi Magnesium Recovery ar Magnesium improverr Recovery ar improverr Magnesium Recovery ar improverr lumsauSeiAl Recovery ar improverr lumsauSBiAl Recovery ar improverr Magnesium Recovery ar improverr tunisauSejAi Recovery ar lOAOJdUJ; uJ CTQ 25 tn 3 No Impr No Impr No Impr idmj on No Impr No Impr -I No Impr X o ■I on c -1 No Impr rduji on 2 Q. 5 3 2 •-t 5' x SM0L|S UOIJS3 on nd 10 mM lent in %Hb 0J o IA d m ound 10 mM fD 5’ 0s X Caron snows ound 10 mM Cat-on shows sent in %Hb ound 10 mM CD rf 1 smous nopem ound 10 mM rent in %Hb Cation snows i ound 10 mM 2 •-t 5' x Cation snows | ound 10 mM n> 5' Cation shows ound 10 mM fD 5’ 0s X Cation snows ound 10 mM Cation shows sent in %Hb ound 10 mM CD rf cation shows ound 10 mM rent in %Hb 1 smolis uoQen ound 10 mM 2 •-t 5' X Cation shows | ound 10 mM n> 5' cation shows ound 10 mM rent in %Hb CD Q in O ound 10 mM fD 5’ 0s X Cation shows ovement ■ovement Q < CD 3 2 Q 3 0) 2- ovement ‘ovement ‘ovement c < ru 3 2 ■ovement Q 2 CD D ovement ‘ovement ■ovement £ io APPENDIX B 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery Resufts Polyvalent lons / Metal Salts Magnesium Sulfate 40 mM 78 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate 50 mM 76 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Aldehydes Formaldehyde Solution 0.010% 54 No Improvement Aldehydes Formaldehyde Solution 0.015% 52 No Improvement Aldehydes Formaldehyde Solution 0.020% 55 No Improvement Aldehydes Formaldehyde Solution 0.025% 53 No Improvement Aldehydes Formaldehyde Solution 0.030% 53 No Improvement Aldehydes Formaldehyde Solution 0.035% 54 No Improvement Aldehydes Formaldehyde Solution 0.040% 55 No Improvement Aldehydes Formaldehyde Solution 0.045% 54 No Improvement Aldehydes Formaldehyde Solution 0.050% 52 No Improvement Aldehydes Formaldehyde Solution 0.055% 53 No Improvement Aldehydes Formaldehyde Solution 0.060% 56 No Improvement Aldehydes Formaldehyde Solution 0.065% 53 No Improvement Aldehydes Formaldehyde Solution 0.070% 53 No Improvement Aldehydes Formaldehyde Solution 0.075% c4 No Improvement Aldehydes Formaldehyde Solution 0.080% 5 b No Improvement Aldehydes Formaldehyde Solution 0.085% 55 No Improvement Aldehydes Formaldehyde Solution 0.090%. 54 No Improvement Aldehydes Formaldehyde Solution 0.095% 53 No Improvement Aldehydes Formaldehyde Solution 0.100% 53 No Improvement Aldehydes Paraformaldehyde Solution 0.010% 46 No Improvement Aldehydes Paraformaldehyde Solution 0.015% 39 No Improvement Aldehydes Paraformaldehyde Solution 0.020% 32 No Improvement Aldehydes Paraformaldehyde Solution 0.025% 33 No Improvement Aldehydes Paraformaldehyde Solution 0.030% 29 No Improvement Aldehydes Paraformaldehyde Solution 0.035% 27 No Improvement Aldehydes Paraformaldehyde Solution 0.040% 24 No Improvement Aldehydes Paraformaldehyde Solution 0.045% 21 No Improvement Aldehydes Paraformaldehyde Solution 0.050% 16 No Improvement Aldehydes Paraformaldehyde Solution 0.055% 15 No Improvement Aldehydes Paraformaldehyde Solution 0.060% 1.4 No Improvement Aldehydes Glutaraldehyde Solution 0.010% 59 No Improvement Aldehydes Glutaraldehyde Solution 0.015% 61 No Improvement Aldehydes Glutaraldehyde Solution 0.020%. 62 No Improvement Aldehydes Glutaraldehyde Solution 0.025% 60 No Improvement Aldehydes Glutaraldehyde Solution 0.030% 56 No Improvement Aldehydes Glutaraldehyde Solution 0.03b% 60 No Improvement Aldehydes Glutaraldehyde Solution 0.040% 61 No Improvement Aldehydes Glutaraldehyde Solution 0.045% 60 No Improvement Aldehydes Glutaraldehyde Solution 0.050% 57 No Improvement Aldehydes Glutaraldehyde Solution 0.055% 107 Assay Interference Aldehydes Glutaraldehyde Solution 0.060% 102 Assay Interference Aldehydes Glutaraldehyde Solution U.0bb% 82 Assay Interference Aldehydes Glutaraldehyde Solution 0.070% ioo Assay Interference Aldehydes Glutaraldehyde Solution 0.075%. 53 No Improvement Aldehydes Glutaraldehyde Solution 0.080% 54 No Improvement Aldehydes Glutaraldehyde Solution 0.085% 54 No Improvement Aldehydes Glutaraldehyde Solution 0.090% 53 No Improvement Aldehydes Glutaraldehyde Solution 0.095% 51 No Improvement Aldehydes Glutaraldehyde Solution 0.100%. 48 No Improvement Formaldehyde Releaser Diazoiidinyi Urea + DMSO + Zinc. Chloride 0.1% (50g / mL, 100 mL / L, 5.8 g / L) 66 No Improvement Formaldehyde Releaser Diazoiidinyi Urea +• DMSO + Zinc Chloride ISO mT7L, 5.8 g / L) 65 No Improvement 2026214068   07 Aug 2026 Compound Additive CorKentratioe Recovery Resufts Formaldehyde Reieaser Diazolidinyl Urea t DMSO + Zinc Chloride (50g / mi, 1® mt / L, 5.3 g / L; 63 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc Chloride 0.75% (50g / mL, 100 mL / L, 5.8 g / L) 69 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc Chloride 1% (50g / mL, 100 mL / L, 5.8 g / L) 64 No Improvement Formaldehyde Release;' Diazolidinyl Urea + DMSO a- Zinc Chloride 2%. (50g / mL, 100 mL / L, 5,8 g / L) 64 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc. Chloride 3% (50g / mL, 100 mL / L, 5.8 g / L) 63 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc Chloride 4% (50g / mL, 100 rnL / L, 5.8 g / L) 64 No Improvement Formaldehyde Releaser Diazolidinyl Urea + DMSO + Zinc Chloride 5% (50g / mL, 100 mL / L, 5.8 g / L) 59 No Improvement Formaldehyde Releaser Imidazolidinyl Urea + DMSO + Zinc Chloride 0.1% (50g / mL, 100 mL / L, 5.8 g / L) 70 No Improvement Formaldehyde Releaser imidazolidinyl Urea + DMSO + Zinc Chloride 0.25% (50g / mL, 100 mL / L, 5.8 g / L) 66 No Improvement Formaldehyde Releaser Imidazolidinyl Urea + DMSO + Zinc Chloride 0.5% (50g / mL, 100 mL / L, 5 8 g / L) 69 No Improvement Formaldehyde Releaser imidazolidinyl Urea + DMSO a- Zinc Chloride 0.75% (50g / mL, 100 rnL / L, 5.3 g / L) 62 No Improvement Formaldehyde Releaser imidazolidinyl Urea + DMSO a- Zinc Chloride 1% (50g / mL, 100 mL / L, 5.8 g / L) 70 No Improvement Formaldehyde Releaser imidazolidinyl Urea + DMSO + Zinc Chloride 2% (50g / mL, 100 mL / L, 5.8 g / L) 65 No Improvement Formaldehyde Releaser imidazolidinyl Urea a- DMSO + Zinc Chloride 3% (50g / mL, 100 mL / L, 5.8 g / L) 66 No Improvement Formaldehyde Releaser imidazolidinyl Urea a- DMSO + Zinc Chloride 4% (50g / mL, 100 mL / L, 5.8 g / L) 63 No Improvement Formaldehyde Releaser imidazolidinyl Urea + DMSO + Zinc Chloride 5% (50g / mL, 100 mL / L, 5.8 g / L) 65 No Improvement Formaldehyde Releaser Diazolidinyl Urea 10 mM 60 No Improvement Formaldehyde Releaser Diazolidinyl Urea 25 mM 61 No Improvement Formaldehyde Releaser Diazolidinyl Urea 50 mM 49 No Improvement Formaldehyde Releaser Diazolidinyl Urea 75 mM 58 No Improvement Formaldehyde Releaser Diazolidinyl Urea 100 mM 50 No Improvement Formaldehyde Releaser imidazolidinyl Urea 10 mM 61 No Improvement Formaldehyde Releaser imidazolidinyl Urea 25 mM 62 No Improvement Formaldehyde Releaser Imidazolidinyl Urea 50 mM 57 No Improvement Formaldehyde Releaser Imidazoiidinyi Urea 75 mM 55 No Improvement Formaldehyde Releaser Imidazolidinyl Urea 100 mM 51 No Improvement Formaldehyde Releaser Soduim hyrdoxyimethyi glycinate 10 mM 48 No Improvement Formaldehyde Releaser Soduim hyrdoxyimethyi glycinate 25 mM 43 No Improvement Formaldehyde Releaser Soduim hyrdoxyimethyi glycinate 50 mM 74 No Improvement Formaldehyde Releaser Soduim hyrdoxyimethyi glycinate 75 mM 53 No Improvement Formaldehyde Releaser Soduim hyrdoxyimethyi glycinate 90 mM 36 No Improvement Osmolytes Betaine 0.05 M 70 Betaine shows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 0.5 M 70 Betaine shows improvemerit in %Hb Recovery at high concentrations Osmolyfes Betaine 1.25 M 75 betaine snows improvement in %Hb Recovery at high concentrations 2026214068   07 Aug 2026 Compound Add)«w« Additive CatxsAtmton Recovery R&sufts Osmolytes Betaine 2.5 M 73 Betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 3.75 M 82 Betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 5 M 80 Betaine snows improvemerit in %Hb Recovery at high concentrations Osmolytes Trimethylamine N-oxide (TMANO) 0.043 M 70 Slight Improvement Osmolytes Trimethylamine N-oxide (TMANO) 0.43 M 70 Slight Improvement Osmolytes Trimethylamine N-oxide (TMANO) 1.1 M 74 Slight Improvement Osmolytes Trimethylamine N-oxide (TMANO) 2.15 M 72 Slight Improvement Osmolytes Trimethylamine N-oxide (TMANO) 3.2 M 74 Slight Improvement Osmolytes Trimethylamine N-oxide (TMANO) 4.3 M 68 Slight Improvement Osmolytes Betaine 2.5 M 72. Betaine snows improvemerit in %Hb Recovery at high concentrations Osmolytes Betaine 3 M 73 Betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 3.5 M 79 Betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 4 M 78 betaine sTiows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 4.5 M 80 betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Betaine 5 M 83 betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Trimethylamine N-oxide (TMANO) 2.5 M 65 No Improvement Osmolytes Trimethylamine N-oxide (TMANO) 3 M 64 No Improvement Osmolytes Trimethylamine N-oxide (TMANO) 3.5 M 73 No Improvement Osmolytes Trimethylamine N-oxide (TMANO) 4 M 69 No Improvement Osmolytes Trimethylamine N-oxide (TMANO) 4.3 M 58 No Improvement Detergent Brij 35 (Component) 6.005% 63 No Improvement Detergent Brij 35 (Component) 0.010% 59 No Improvement Detergent Brij 35 (Component) 0.050% 69 No Improvement Detergent Brij 35 (Component) 0.100% 62 No Improvement Detergent Brij 35 (Component) 0.200% 57 No Improvement Detergent Brij 58 (Component) 0.005% 63 No Improvement Detergent Brij 58 (Component) 0.010% 67 No Improvement Detergent Brij 58 (Component) 0.050% 65 No Improvement Detergent Brij 58 (Component) 6.106% 63 No Improvement Detergent Brij 58 (Component) 0.200% 49 No Improvement Detergent Brij 35 (Spike) 0.010% 61 No Improvement Detergent Brij 35 (Spike) 0.050%. 59 No Improvement Detergent Brij 35 (Spike) 0.080% 59 No Improvement 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery Resufts Deter ger.: Brij 35 (Spike) 0.100% 58 No Improvement Detergent Brij 58 (Spike) 0.010% 65 No Improvement Detergent Brij 58 (Spike) 0.050% 61 No Improvement Detergent Brij 58 (Spike) 0.080% 59 No Improvement Detergent Brij 58 (Spike) 0.100% 53 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride 0.001 M 62 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride 0.61 M 68 No Improvement Polyvalent lons / Metal Saits Cobalt Chloride 0.05 M 16 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Sucrose 0,001 M + 2% 73 siignt improvement witn Sugars. Needs further assay optimization Polyvalent lons / Metal Salts Cobalt Chloride + Sucrose 0.01 M + 2% 77 Slight improvement with Sugars. Needs further assay optimization Polyvalent lons / Metal Salts Cobait Chloride + Sucrose 0.05 M + 2% 17 Slight Trh pfbvemenf wit ri Sugars. Needs further assay optimization Polyvalent lons / Metal Salts Magnesium Sulfate 0.001 M 66 Magnesium cation snows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate 0.01 M 75 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate 0.05 M 78 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Sucrose 0,001 M + 2% 72 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Sucrose 0.01 M + 2% 81 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Sucrose 0.05 M + 2% 80 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Copper Chloride 1 mM 64 No Improvement Polyvalent lons / Metal Saits Copper Chloride 10 mM 63 No Improvement Polyvalent lons / Metal Salts Copper Chloride 25 mM 42 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Sucrose 1 mM + 2% 69 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Sucrose 10 m M + 2% 69 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Sucrose 25 mM + 2% 47 No Improvement Polyvalent lons / Metal Saits Iron (3) Chloride 1 mM 60 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride 5 mM 61 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride 10 mM 66 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Sucrose 1 mM + 2% 65 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Sucrose 5 mM + 2% 65 No Improvement Polyvalent lons / Metal Saits Iron (3) Chloride + Sucrose 1.0 mM + 2% 59 No Improvement Polyvalent lons / Metal Saits Nickel Chloride 1 mM 59 No Improvement Polyvalent lons / Metal Salts Nickel Chloride 10 mM 68 No Improvement Polyvalent lons / Metal Salts Nickel Chloride 25 mM 32 No Improvement Polyvalent lons / Metal Salts Nickel Chloride + Sucrose 1 mM + 2% 63 No Improvement Polyvalent lons / Metal Salts Nickel Chloride -f Sucrose 10 mM + 2% 68 No Improvement Polyvalent lons / Metal Saits Nickel Chloride + Sucrose 25 mM + 2% 33 No Improvement Polyvalent lons / Metal Salts Zinc Chloride 1 mM 65 No Improvement Polyvalent lons / Metal Salts Zinc Chloride id mM 61 No Improvement Polyvalent lons / Metal Salts Zinc Chloride 25 mM 33 No Improvement Polyvalent lons / Metal Salts Zinc Chloride + Sucrose 1 mM + 2% 68 No Improvement Polyvalent lons / Metal Saits Zinc Chloride + Sucrose 10 mM + 2%. 75 No Improvement Polyvalent lons / Metal Salts Zinc Chloride + Sucrose 25mM + 2% 36 No Improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery R&sufts Polyvalent lons / Metal Salts Calcium Chloride 5 mM 66 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride 10 mM 65 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride 20 mM 69 CaTcTum cation sTiows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride 30 mM 59 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium: Chloride 40 mM 62 caicium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride 50 mM 52 Caicium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Propionate 5 mM 62 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium: Propionate 10 mM 61 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Propionate 20 mM 70 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Propionate 30 mM 66 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Propionate 40 mM 62 Caicium cation show's improvement in %Hb Recovery at 10 rnM Polyvalent lons / Metal Salts Calcium Propionate 50 mM 62 Caicium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Cobalt Chloride 5 mM + 5 mM 68 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Cobalt Chloride 10 mM 5 mM 60 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Cobalt Chloride 5 mM + 10 mM 58 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride + Copper Chloride 5 mM + 5 mM 66 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride + Copper Chloride 10 mM- ■ 5 mM 50 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Copper Chloride 5 mM + 10 mM 59 Caicium cation show's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + iron (3) Chloride 5 mM + 5 mM 76 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + iron (3) Chloride 5 mM + 2,5 mM 63 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium: Chloride + iron (3) Chloride 2.5 mM - 5 mM 63 calcium cation snows improvement in %Hb Recovery at 10 mM 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 5 miM + 5 mM 71 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 10 mM + 5 mM 67 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride + Magnesium Sulfate 5 mM + 10 mM 61 Calcium cation sTiows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Nickel Chloride 5 mM + 5mM 69 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride -s- Nickel Chloride 10 mM + 5 mM 64 caicium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Nickel Chloride 5 mM + 10 mM 73 Caicium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Zinc Chloride 5 mM + 5 mM 68 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium: Chloride + Zinc Chloride 10 miM + 5 mM 66 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride + Zinc Chloride 5 mM + 10 mM 63 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Cobait Chloride <- Copper Chloride 5 mM + 5 mM 59 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Copper Chloride 10 m M + 5 m M 47 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Copper Chloride 5 mM + 10 mM 40 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Iron (3) Chloride 5 mM + 5mM 66 No Improvement Polyvalent lons / Metal Saits Cobait Chloride + Iron (3) Chloride 5 mM + 2.5 mM 68 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Iron (3) Chloride 2.5 mM + 5 miM 65 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Magnesium Sulfate 5 mM + 5 mM 74 No Improvement Polyvalent lons / Metal Salts Cobait Chloride + Magnesium Sulfate 10 mM + 5 mM 66 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Magnesium: Sulfate 5 mM + 10 mM 70 No Improvement Polyvalent lons / Metal Saits Cobalt Chloride + Nickel Chloride 5 mM + 5 mM 75 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Nickel Chloride 10 mM + 5 mM 49 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride t Nickel Chloride 5 mM + 10 mM 43 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Zinc Chloride 5 m M + 5 m Ml 71 No Improvement Polyvalent lons / Metal Salts Cobalt Chloride + Zinc Chloride 10 mM + 5 mM 35 No Improvement Polyvalent lons / Metal Saits Cobalt Chloride + Zinc Chloride 5 mM + 10 mM 34 No Improvement Polyvalent lons / Metal Salts Copper Chloride + iron (3) Chloride 5 mM + 5 mM 67 No Improvement Polyvalent lons / Metal Salts Copper Chloride + iron (3) Chloride 5 mM + 2.5 mM 61 No Improvement Polyvalent lons / Metal Salts Copper Chloride + iron (3) Chloride 2.5 mM + 5 mM 70 No Improvement 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery R&sufts Polyvalent lons / Metal Salts Copper Chloride + Magnesium Sulfate 5 mM + 5 mM 70 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Magnesium Sulfate 10 mM 5 mM 63 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Magnesium Sulfate 5 mM + 10 mM 68 No Improvement Polyvalent lons / Metal Salts Copper Chloride a- Nickel Chloride 5 mM + 5 mM 68 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Nickel Chloride 10 m M + 5 m M 42 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Nickel Chloride 5 mM + 10 mM 44 No Improvement Polyvalent lons / Metal Salts Copper Chloride + Zinc Chloride 5 mM + 5mM 68 No Improvement Polyvalent lons / Metal Saits Copper Chloride + Zinc Chloride 10 mM + 5 mM 38 No Improvement Polyvalent lons / Metal Salts Copper Chloride -t Zinc Chloride 5 mM + 10 mM 55 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Magnsium Sulfate 5 mM + 5 mM 71 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Magnsium Sulfate 5 mM + 2.5 mM 68 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Magnsium Sulfate 2.5 mM + 5 mM 63 No Improvement Polyvalent lons / Metal Saits Iron (3) Chloride + Nickel Chloride 5 mM + 5 mM 63 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Nickel Chloride 5 mM + 2.5 mMI 58 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Nickel Chloride 2.5 mM + 5 mM 71 No Improvement Polyvalent lons / Metal Salts iron (3) Chloride + Zinc Chloride 5 mM + 5 mMI 61 No Improvement Polyvalent lons / Metal Salts Iron (3) Chloride + Zinc Chloride 5 mM + 2.5 mM 56 No Improvement Polyvalent lons / Metal Saits Iron (3) Chloride + Zinc Chloride 2,5 mM + 5 mM 66 No Improvement Polyvalent lons / Metal Salts Magnesium Sulfate + Nickel Chloride 5 mM + 5 mM 73 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Nickel Chloride 10 mM + 5 mM 68 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Nickel Chloride 5 mM tlOmM 70 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Zinc Chloride 5 mM + 5 mM 74 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate + Zinc Chloride 10 mM + 5 mM 73 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Saits Magnesium Sulfate + Zinc, Chloride 5 mM + 10 mM 74 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent lons / Metal Salts Nickel Chloride + Zinc Chloride 5 mM + 5 mM 74 No Improvement Polyvalent lons / Metal Salts Nickel Chloride + Zinc Chloride 10 mM + 5 mM 47 No Improvement Polyvalent lons / Metal Salts Nickel Chloride + Zinc Chloride 5 mM + 10 mM 35 No Improvement HP.P Stabilizer Components HRP Conjugate Stabilizer (PN abl71537j (spike) 5% 82 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) (spike) 10% 77 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) (spike) 25% 7b improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive Additive CotxsAtmton Bemvery Resufts HRP Stabilizer Components &oKad AobuarS iita&'hration tiutter (spike) 5% 72 Siignt Improvement. Needs further assay optimization HRP Stabiiizer Components BioRad AbGuard HRP Stabilization Buffer (spike) 10% 78 Slight Improvement. Needs further assay optimization HRP Stabiiizer Components BioRad AbGuard HRP Stabilization Buifer (spike) 25% 85 Slight Improvement. Needs further assay optimization HRP Stabilizer Components Fisher HRP Sol (spike) 5% 65 No Improvement HRP Stabilizer Components Fisher HRP So! (spike) 10% 62 No Improvement HRP Stabilizer Components Fisher HRP So! (spike) 25% 57 No Improvement HRP Stabiiizer Components ICTech (spike) 5% 64 No Improvement HRP Stabiiizer Components IC Tech (spike) 10% 68 No Improvement HRP Stabilizer Components IC Tech (spike) 25% 65 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZO2) (spike) 5%, 78 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (spike) 10% 86 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (spike) 25% 82 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) (component) 1% 69 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN ab!71537) (component) 2.50% 67 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) (component) 5%, 70 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (comp) 1% 67 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (comp) 2.50% 69 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (comp) 5% 69 improvement in %Hb Recovery HRP Stabilizer Components Fisher HRP So! (comp) 1% 65 No Improvement HRP Stabilizer Components Fisher HRP Sol (comp) 2.50% 65 No Improvement HRP Stabilizer Components Fisher HRP Sol (comp) 5% 64 No Improvement HRP Stabilizer Components ID Stabilization Buffer (comp) 1% 67 No Improvement HRP Stabilizer Components ID Stabilization Buffer (comp) 2.50% 66 No Improvement HRP Stabilizer Components ID Stabilization Buffer (comp) 5% 65 No Improvement HRP Stabilizer Components BioRad AbGuard HRP Stabilization Butter (comp) 10% 74 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer (comp) 25% 83 Slight Improvement. Needs further assay optimization HRP Stabilizer Components BioRad AbGuard HRP Stabilization Buffer (comp) 40% 90 Slight Improvement. Needs further assay optimization HRP Stabilizer Components ICTech (comp) 1% 67 No Improvement HRP Stabilizer Components IC Tech (comp) 5% 67 No Improvement HRP Stabilizer Components ICTech (comp) 10% 67 No Improvement Polyvalent lons / Metal Salts Calcium Propionate + PEG 8000 0.1% i- 3% 64 No improvement Polyvalent lons / Metal Salts Calcium Propionate + PEG 8000 0.2% + 3% 63 No improvement Polyvalent lons / Metal Salts Calcium Propionate + PEG 8000 0.3% + 3% 66 No improvement Polyvalent lons / Metal Salts Calcium Propionate + Calcium Chloride + PEG 8000 0.1% + 3%. 65 No improvement Polyvalent lons / Metal Saits Calcium Propionate + Calcium Chloride + PEG 8000 0.2% + 3% 68 No improvement Polyvalent lons / Metal Salts Calcium Propionate + Calcium Chloride r PEG 8000 0.3% i- 3% 62 No improvement HRP Stabilizer Components 4-Bromophenol 0.001%. 59 No Improvement HRP Stabilizer Components 4-Bromophenol 0.005% 59 No Improvement HRP Stabilizer Components 4-Bromophenol 0.61% 48 No Improvement HRP Stabilizer Components 4-Bromophenol 0.05% 54 No Improvement 2026214068   07 Aug 2026 Compound Additive Recovery Resufts HRP Stabilizer Components 8-an:!:no-l-napthalene sultomc acid (ANSA) 0.01% 61 No Improvement HRP Stabiiizer Components 8-anilino-l-napthaiene sulfonic acid (ANSA) 0,05% 68 No Improvement HRP Stabiiizer Components 8-anilino-l-napthalene sulfonic acid (ANSA) 0.10% 59 No Improvement HRP Stabilizer Components 8-aniiino-i-napthalene sulfonic acid (ANSA) 0.50% 42 No 1 improvement HRP Stabilizer Components Prociin 300 0.00005% 61 No Improvement HRP Stabilizer Components Prociin 300 0.0001% 61 No Improvement HRP Stabilizer Components Prociin 300 0.0002% 55 No Improvement Poiyvaient ions / Metai Saits Cobalt Chloride 0.1 mM 67 No Improvement Poiyvaient lons / Metal Salts Cobalt Chloride 1 rnM 71 No Improvement Poiyvaient ions / Metai Salts Cobalt Chloride 10 mM 63 No Improvement Poiyvaient lons / Metal Salts Cobalt Chloride + Sucrose 0.1 mM + 2% 69 No Improvement Poiyvaient lons / Metal Salts Cobalt Chloride + Sucrose 1 mM + 2% 69 No Improvement Poiyvaient ions / Metai Saits Cobalt Chloride + Sucrose 10 mM + 2% 74 No Improvement Poiyvaient lons / Metal Salts Magnesium Sulfate 0.1 rnM 73 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Poiyvaient ions / Metai Salts Magnesium Sulfate 1 mM 73 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent ions / Metai Salts Magnesium Sulfate 10 mM 56 Magnesium: Cation snows improvement in %Hb Recovery around 10 mM Polyvalent ions / Metai Salts Magnesium Sulfate + Sucrose 0,1 mM + 2%. 79 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Polyvalent ions / Metai Salts Magnesium Sulfate + Sucrose lmM + 2% 68 Magnesium Cation shows improvement in %Hb Recovery around 10 mM Poiyvaient ions / Metai Saits Magnesium Sulfate + Sucrose 10 mM + 2% 71 Magnesium Uation shows improvement in %Hb Recovery around 10 mM HRP Stabilizer Components Candor HRP Protector (spike) 25% 65 No Improvement HRP Stabilizer Components Candor HRP Protector (spike) 10% 61 No Improvement HRP Stabilizer Components Candor HRP Protector (spike) 5% 62 No Improvement HRP Stabilizer Components Innova LifeXtend Stabilizer (spike) 25% 62 No Improvement HRP Stabilizer Components Innova LifeXtend Stabilizer (spike) 10% 68 No Improvement HRP Stabilizer Components Innova LifeXtend Stabilizer (spike) 5% 63 No Improvement Aldehydes Acetaldehyde 10 rnM 56 No Improvement Aldehydes Acetaldehyde 25 mM 48 No Improvement Aldehydes Acetaldehyde 50 mM 43 No Improvement Aldehydes Benzaldehyde 10 mM 46 No Improvement Aldehydes Benzaldehyde 25 mM 28 No Improvement Aldehydes Benzaldehyde 50 rnM 53 No Improvement Aldehydes Butyraldehyde 10 mM 47 No Improvement Aldehydes Butyraldehyde 25 mM 34 No Improvement Aldehydes Butyraldehyde 50 mM 30 No Improvement Aldehydes Cinnamaidehyde 10 mM 25 No Improvement Aldehydes Cinnamaidehyde 25 mM 32 No Improvement Aldehydes Cinnamaidehyde 50 mM 21 No Improvement Aldehydes Formamide 10 mM 62 No Improvement Aldehydes Formamide 25 mM 62 No Improvement Aldehydes Formamide 50 mM 62 No Improvement Aldehydes Furfural 10 mM 58 No Improvement Aldehydes Furfural 25 rnM 37 No Improvement 2026214068   07 Aug 2026 > CL re cL 0) tn > re Cl re Aldehydes Aldehydes Aldehydes S "sore tn sepAqspjv Aldehydes Aldehydes Aldehydes S cL 0) Ln sapAqapiv CL re Q. re Aldehydes > CL re re" tn CL re 'o. re tn re 1L re Ln > CL re Cl re Aldehydes sspAqapiv > CL re re" tn s "ci re Ln sapAqapiv         I Aldehydes Aldehydes Aldehydes CL re "re. re tn Aldehydes sepAqapjv Aldehydes sapAqapiv         | CL re 're. re tn re 're-re tn > CL re Q-re Aldehydes Aldehydes > CL re re" tn re "ci re tn sspAqspiv         I Aldehydes Aldehydes Aldehydes CL re "cL re tn Aldehydes sepAqspjv Aldehydes sspAqspiv         | CL re 'q. re tn re Q_ re tn > CL re Q. re Aldehydes Aldehydes > CL re re" tn re "o_ re tn sspAqspiv         I Aldehydes Aldehydes Aldehydes CL re S t« f : «> § i i to : Gr b GJ o 3 CD 3 S' Q 3 CD 3 n. CD o 3 3 S' c 3 CD S O 3 CD 3 al re o 3 CD 3 S' "o © 3’ S (Q p 3' re p 8 3’ cl Q. Q P 3' re "n O_ 3’ CL G S' 3 2 O 8 S Q. CD P re o s S' 3 re Q "o © 3’ S S p 3' re p th re cd Q. fD Q. re re GJ S’ CL re Q ch re S CL re p ch re cl re_ S 9 o re aj S' 'll re c ch re re Q re "re-fi? 3 re S' s re re 3 re "ci. re Q re' re re re a. re_ S re re 3 CL re_ "a. re Q 3 re Q. re_ Q. re Q re 3 re Q. .¾. CL re <-}■ re CL re re-re P re CL re re. re □3 re S re" □j re CL re re" P re "re-re CD rn- re re-re re-re 03 re isj SU CL re 're. re CD re IN GJ re re-re CD re CD CL re "re-re CO re reT re "re. re CD re S re" CD re iXJ re CL re re" > re re 32 "q. re re S' Q. re re re Q. re re re re^ Q. re re re of S’ re re re 3" re* 5’ 3. 5' '9 o' c CD S' s s' re' Q. re ■3 a E re_ re tn C s 8 CL re re" G E re re CL re s -p> 0 re- d: 3 re" re" CT) "n o_ re’ CL re S’ 3‘ re O 8 s Q. re 9 re 8 re” s S' 3' re 9 ch re re Q. re_ "a. re 9 th re re Q. .¾. Q_ re re CD re” CL re iT <2 re & : III o -3 o 3 Ul 3 o 2 F-1 3 p 3 'q 3 m o 1 o 3 F-1 3 p 3 Ul o 3 Ul 3 o 3 F"1 3 3 o 3 o U1 3 3 }--> 3 p 3 £ o b 3 o 3 NJ Ui 3 5 3 3 o 3 p 3 Ui o 3 NJ Ui 3 b 3 S 3 b 3 u 3 tn 3 o | i-j, 3 3 o b S Ui 0 3 In 0 3 0 NJ Ui 3 3 0 NJ Ul 0 0 3 NJ Ui 3 5 3 Ul 0 ui 3 iS 0 3 Ui 3 & 1 n : » «> 1 & Xi CD p CD NJ NJ CD ‘3» Ci s NJ $ o s CD i--'' Cl LU Ci o CD CD a M Ln in UI UJ 4N to w la Ui a Ui UJ 00 O's co a in Ul cu 00 UI CD CD ai NJ Ul UI 4N O w Ul Ui 00 s •u N) F-1 CD OJ UJ 2 co o •I o re 3 re o 3 C5 a re 3 0) 25 C -1 o re 3 re 25 o 3 '3 6 < re 3 re 25 o c tn 3 re o c < re 3 07 2: o 3 <5 a re 3 0) 23 -I a re a> D o 3 '3 d < ro 3 re 15 -I d 0) 3 0) 25 o c < re 3 re o •I o re 3 re -I o re re D c 1 o re 3 re 23 -I d re 3 re 25 O © re 3 re o c < re 3 re 2C O 3 <5 Q re 3 re 25 -I c re re re O 3 '3 d < re 3 re 25 -I d re 3 re 25 O c < re 3 re o ■I o ri) 3 re -I Q re re re c -1 o re 3 re re -I d re 3 re 25 o d re 3 o •I o ri) 3 re 2C O 3 cs Q re 3 re 25 c -1 o re 3 re 25 o 3 '3 d < re 3 re 25 o d re 3 o c < re 3 re 2C O 3 cs Q re 3 re 25 Q re re 5 o 3 ’3 d < re 3 re 25 -I d re 3 re 25 o c < re 3 re o ■I o ri) 3 re -l Q re re o c -1 o re 3 re 25 -I d re 3 re 25 o d re 3 0 •I 0 ri) 3 re 2: 0 3 C5 Q re 3 re 25 C -1 O re 3 re 25 0 3 '3 d < re 3 re 25 0 d re 3 0 c < re 3 re 2: 0 3 ‘C5 Q re 3 re 25 -1 Q re re D 0 3 '3 d < re 3 re 25 -I d re 3 re 25 O 8 < re 3 re 0 ■I 0 ri) 3 re -l Q re re D C -1 O re 3 re 25 -I d re 3 re 25 0 0 re 3 re X : i CD APPENDIX B 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery Resufts Aldehydes Furfural 0.1 mM bb No Improvement Aldehydes Furfural 1 mM 34 No Improvement Aldehydes Furfural 10 mM 58 No Improvement Aldehydes FurlUTa I 25 mM 37 No Improvement Aldehydes Furfural 50 mM 38 No Improvement Aldehydes 4-lsobutyi benzaldehyde 0.01 mM 69 No Improvement Aldehydes 4-lsobutyibenzaldehyde 0.1 mM 6i No Improvement Aldehydes 4-lsobutyl benzaldehyde 1 mM 31 No Improvement Aldehydes 4-lsobutylbenzaldehyde 10 mM 57 No Improvement Aldehydes 4-lsobutylbenzaldeh yd e 25 mM 44 No Improvement Aldehydes 4-lsobutyl benzaldehyde 50 mM 48 No Improvement Aldehydes p-Tolualdehyde 0.01 mM 65 No Improvement Aldehydes p-Tolualdehyde 0.1 mM 62. No Improvement Aldehydes p-Tolualdehyde 1 mM 35 No Improvement Aldehydes p-Tolualdehyde IO mM 51 No Improvement Aldehydes p-Tolualdehyde 25 mM 35 No Improvement Aldehydes p-Tolualdehyde 50 mM 71 No Improvement Aldehydes Vanillin 0.01 mM 66 No Improvement Aldehydes Vanillin 0.1 mM 58 No Improvement Aldehydes Vanillin 1 mM 32 No Improvement Aldehydes Vanillin 10 mM 40 No Improvement Aldehydes Vanillin 25 mM 32 No Improvement Aldehydes Vanillin 50 mM 34 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 5% 78 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 10% 80 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 25% improvement in %Hb Recovery Formaldehyde Releaser Bronidox 5 mM 47 No Improvement Formaldehyde Releaser Bronidox 10 mM 27 No Improvement Formaldehyde Releaser Bronidox 25 mM 37 No Improvement Formaldehyde Reieaser Bronopol 5 mM 34 No Improvement Formaldehyde Releaser Bronopol 16 mM 31 No Improvement Formaldehyde Releaser Bronopol 25 mM 24 No improvement Preservatives / Parabens Benzyl 4-hydroxybenzoate 5 mM 63 No improvement Preservatives / Parabens Benzyl 4-hydroxybenzoate 10 mM 35 No Improvement Preservatives / Parabens Benzyl 4-hydroxybenzoate 25 mM 26 No Improvement Preservatives / Parabens Ethv! 4-hydroxybenzoate 5 mM 54 No Improvement Preservatives / Parabens Ethyl 4-hydroxybenzoate 10 mM 43 No improvement Preservatives / Parabens Ethyl 4-hydroxybenzoate 25 mM 30 No improvement Preservatives / Parabens Methyl 4-hydroxybenzoate 5 mM 58 No Improvement P res erva t i ves / Pa ra be ns Methyl 4-hydroxybenzoate 10 mM 44 No Improvement Preservatives / Parabens Methyl 4-hydroxybenzoate 25 mM 34 No Improvement Preservatives / Parabens Propyl 4-hydroxybenzoate 5 mM 55 No Improvement Preservatives / Parabens Propyl 4-hydroxybenzoate 10 mM 37 No improvement Preservatives / Parabens Propyl 4-hydroxybenzoate 25 mM 28 No improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 1% 69 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 5% 79 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 10% 76 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 81 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 20% 75 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 25% 76 improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive Additive Cancentmion Recovery Resufts HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 30% 70 imp coven": ent in '^>Ab Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102) 40% 71 improvement in %Hb Recovery Polyvalent lons / Metal Salts Aluminum Chloride 2.5 mM 69 No Improvement Polyvalent lons / Metal Salts Aluminum Chloride 5 mM 70 No Improvement Poiyvaient lons / Metal Salts Aluminum Chloride 10 mM 29 No Improvement Formaldehyde Releaser Quaternium 15 5 mM 52 No Improvement Formaldehyde Releaser Quaternium 15 W mM 47 No Improvement Formaldehyde Reieaser Quaternium 15 20 mM 50 No Improvement Preservatives Sodium Nitrate 5 mM 70 No Improvement Preservatives Sodium Nitrate 10 mM 65 No Improvement Preservatives Sodium Nitrate 20 mM 56 No Improvement Preservatives Sodium Nitrite 5 mM 64 No Improvement Preservatives Sodium Nitrite 10 mM 65 No Improvement Preservatives Sodium Nitrite 20 mM 62 No Improvement Substrates Styrene Glycol 5 mM 56 No Improvement Substrates Styrene Glycol 10 mM 58 No Improvement Substrates Styrene Glycol 20 mM 48 No Improvement Substrates Styrene Oxide 5 mM 57 No Improvement Substrates Styrene Oxide 10 mM 42 No Improvement Substrates Styrene Oxide 20 mM 31 No Improvement Enzyme Inhibitor aurintircarboxylic. acid ammonium salt 0.1 mM 52 No Improvement Enzyme Inhibitor aurintircarboxylic acid ammonium salt 1 mM 63 No Improvement Enzyme Inhibitor aurintircarboxylic acid ammonium salt 10 mM 35 No Improvement Polyvalent lons / Metal Saits Sodium Citrate 0.1 mM 61 No Improvement Polyvalent lons / Metal Salts Sodium Citrate 1 mM 60 No Improvement Poiyvaient lons / Metal Salts Sodium Citrate 10 mM 55 No Improvement Vitamins Vitamin B2 0.002 mM 58 No Improvement Vitamins Vitamin B2 0.02 mM 48 No Improvement Vitamins Vitamin B2 0.2 mM 45 No Improvement Vitamins Vitamin B3 0.1 mM 53 No Improvement Vitamins Vitamin B3 1 mM 58 No Improvement Vitamins Vitamin B3 10 mM 42 No Improvement Vitamins Vitamin B4 0.1 mM 54 No Improvement Vitamins Vitamin B4 1 mM 57 No Improvement Vitamins Vitamin B4 10 mM 27 No Improvement Vitamins Vitamin B9 0.1 mM 58 No Improvement Vitamins Vitamin B9 1 mM 43 No Improvement Vitamins Vitamin B9 10 mM 37 No Improvement Vitamins NAD 6.05 mM 59 No Improvement Vitamins NAD + 0.5 mM 54 No Improvement Vitamins NAD + 5 mM 57 No Improvement Vitamins NADH 0.1 mM 63 No Improvement Vitamins NADH 1 mM 66 No Improvement Vitamins NADH 10 mM 65 No Improvement Vitamins Vitamin B12 0.01 mM 53 No Improvement Vitamins Vitamin B12 0.1 mM 62 No Improvement Vitamins Vitamin B12 1 mM 59 No Improvement Vitamins Vitamin B6 0.1 mM 55 No Improvement Vitamins Vitamin BS 1 mM 53 No Improvement Vitamins Vitamin B6 10 mM 58 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 10% 77 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 74 Improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 20% 78 improvement in Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102) 25% 74 improvement in %Hb Recovery Formaldehyde Releaser BSA, Bronidox, Methyiisothiazolone, Proclin 300 (10 mM MOPS) 10% 67 No Improvement Formaldehyde Releaser BSA, Bronidox, Methyiisothiazolone, Proclin 300 (10 mM MOPS) 15% 62 No improvement Formaldehyde Releaser BSA, Bronidox, Methyiisothiazolone, Proclin 300 (10 mM MOPS) 20% 66 No Improvement Formaldehyde Releaser BSA, Bronidox, Methyiisothiazolone, Proclin 300 (10 mM MOPS) 25% 61 No Improvement Formaldehyde Reieaser Bronidox, Methyiisothiazolone, Prolin 300 (lOmM MOPS) 0.002%, 0.002%, 0.0002% 64 No Improvement Formaldehyde Releaser Bronidox, Methyiisothiazolone, Prolin 300 (lOmM MOPS) 0.003%, 0.003%, 0.0003% 57 No improvement Formaldehyde Releaser Bronidox, Methyiisothiazolone; Prolin 300 (10m M MOPS) 0.004%, 0.004%, 0.0004% 52 No Improvement Formaldehyde Releaser Bronidox, Methyiisothiazolone, Prolin 300 (lOmM MOPS) 0.005%, 0.005%, 0.0005% 45 No Improvement Formaldehyde Releaser Bronidox, Methyiisothiazolone (10 mM MOPS) 0.002%, 0.002% 64 No Improvement Formaldehyde Releaser Bronidox, Methyiisothiazolone (10 mM MOPS) 0,003%, 0,003% 58 No Improvement Formaldehyde Reieaser Bronidox, Methyiisothiazolone (10 mM MOPS) 0.004%, 0.004% 56 No Improvement Formaldehyde Reieaser Bronidox, Methyiisothiazolone (10 mM MOPS) 0.005%, 0.005% 53 No improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 10% 75 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 72 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 20% 75 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 25% 63 improvement in %Hb Recovery Formaldehyde Reieaser Bronidox 0,002% 67 No Improvement Formaldehyde Reieaser Bronidox 0.003% 65 No Improvement Formaldehyde Reieaser Bronidox 0.004% 69 No Improvement Formaldehyde Reieaser Bronidox 0.005% 65 No Improvement Enzyme Inhibitor Methyiisothiazolone 0.002% 54 No Improvement Enzyme Inhibitor Methyiisothiazolone 0,003% 58 No Improvement Enzyme inhibitor Methyiisothiazolone 0.004% 5 b No improvement Enzyme Inhibitor Methyiisothiazolone 0.005% 47 No Improvement HRP Stabilizer Components Proclin 300 0,0002%. 68 No Improvement HRP Stabilizer Components Proclin 300 0.0003% 68 No Improvement HRP Stabilizer Components Proclin 300 0.0004% 69 No Improvement HRP Stabilizer Components Proclin 300 0.0005% 63 No improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 10% 81 improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Adddivs Additive CotxsAtmton Recovery Resufts HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 74 imp coven": ent in Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102) 20% 70 improvement in %Hb Recovery Formaldehyde Releaser BSA, Bronidox, Methylisothiazolone, Proclin 300 (10 mM MOPS) 10%, (1%, 0,02%, 0,02%, 0.002%) 47 No Improvement Formaldehyde Releaser BSA, Bronidox, Methylisothiazolone, Proclin 300 (10 mM MOPS) 15% (1%, 0.02%, 0.02%, 0.002%) 43 No Improvement Formaldehyde Releaser BSA, Bronidox, Methylisothiazolone, Proclin 300 (10 mM MOPS) 20% (1%, 0.02%, 0.02%, 0.002%) 39 No Improvement Formaldehyde Releaser Bronidox, Methylisothiazolone, Prolin 300 (lOmM MOPS) 10% ( 0,02%., 002%, 0.002%) 52 No Improvement Formaldehyde Reieaser Bronidox, Methylisothiazolone, Prolin 300 (lOmM MOPS) 15% ( 0.02%, 0.02%, 0.002%) 42 No Improvement Formaldehyde Releaser Bronidox, Methylisothiazolone, Prolin 300 (lOmM MOPS) 20% ( 0.02%, 0.02%, 0.002%) 37 No Improvement Formaldehyde Releaser Bronidox, Methylisothiazolone, Prolin 300 10%, ( 0.02%, 0.02%,, 0.002%) 56 No Improvement Formaldehyde Releaser Bronidox, Methylisothiazolone, Prolin 300 15% ( 0.02%, 0.02%, 0.002%) 41 No Improvement Formaldehyde Releaser Bronidox, Methylisothiazolone, Prolin 300 20% ( 0.02%, 0.02%, 0.002%) 45 No Improvement Plasma BSA (lOmM MOPS) 1% 76 No Improvement Polyvalent lons / Metal Salts Calcium Chloride 10 mM Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 73 Magnesium cation snows improvement in %Hb Recovery around 10 mM HRP Stabilizer Components HRP Conjugate Stabilizer (PN ab!71537) 15% 76 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) + Calcium Chloride 15%. + 10 mM 87 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) + Magnesium Sulfate 15% 4 10 m M 89 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) + RD Hb Collection Buffer 15% + 4% 76 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 15% 75 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + Calcium Chloride 15%. + 10 mM 87 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZU2) ■+ Magnesium Sulfate 15%+ 10 mM 92 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + RD Hb Collection Buffer 15% + 4% 77 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 77 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 92 improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive Additive CotxsAirstitm Recovery R&sufts HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15%+ 10 mM 95 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + RD Hb Collection Buffer 15% + 4% 77 improvement in %Hb Recovery HRP Stabilizer Components DEAF. Dextran, Ethylene Glycol, Calcium Chloride 0.5%, 10%, 10 mM 63 No Improvement HRP Stabilizer Components L.s. Dextran, Ethylene Glycol, Calcium Chloride 0.5%, 10%, 10 mM 52 No improvement Suga rs / Sa ccha rides Sucrose 0.5 M 77 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Sucrose 1 M 91 Slight improvement with Sugars, Needs further assay optimization Suga rs / Sa ccha rides Sucrose 2 M 129 Slight improvement with Sugars, Needs further assay optimization Suga rs / Saccha rides Raffinose 0.125 M 71 Slight improvement with' Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Raffinose 0.25 M 74 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Raffinose 0.5 M 87 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Glucose 0.25 M + 0.25 M 78 Slight7 i m pfovemehf wit ri Sugars. Needs further assay optimization Suga rs / Sa etna rides Sucrose + Glucose 0.5 M + 0.5 M 93 Siignt improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Glucose 1 M + 1 M 100 Slight improvement with Sugars, Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Trehalose 0.25 M +0.125 M 74 Slight improvement with' Sugars. Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Trehalose 0.5 M + 0,25 M 75 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Sucrose + Trehalose 1 M + 0.5 M 141 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Raffinose 0.25 M + 0.062.5 M 72 SightTm provemenf win Sugars. Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Raffinose 0.5 M + 0.125 M 85 rsiignt improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Sucrose + Raffinose 1 M + 0.25 M 104 Slight improvement with Sugars, Needs further assay optimization Suga rs / Sa ccha rides Raffinose + Trehalose 0.062.5 M + 0.125 M 71 Slight improvemehf with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Raffinose + Trehalose 0.125 M +0.25 M 88 Slight improvement with Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Additive Cancentmten Recovery Resufts Sugars / Saccharides Raffinose + Trehalose 0.25 M + 0,5 M 79 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Glucose 0.0625 M + 0.25 M 70 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Raffinose + Glucose 0.125 M + 0.5 M 77 Slight imprbvemehf with’ Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Glucose 0.25 M + 1 M 90 siignt improvement witn Sugars. Needs further assay optimization Enzyme Inhibitor aurintircarboxylic acid ammonium salt 5% 66 No improvement Enzyme Inhibitor aurintircarboxylic acid ammonium salt 10% 61 No improvement Enzyme Inhibitor aurintircarboxylic acid ammonium salt 25% 59 No improvement Sugars / Saccharides Raffinose 0.05 M 69 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.1 M 71 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.15 M 73 Slight improvement with" Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0,2 M 80 siignt improvement witn Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.25 M 73 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.3 M 76 Slight7 i m pfovemehf with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.35 M 80 siignt improvement witn Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.4 M 89 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.45 M 69 Slight imprbvemehf with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0,5 M 91 siignt improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Sucrose 0.03 M 67 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose 0.06 M 72 SfigfifTrh pfovemehf with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose 0.09 M 68 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose 0.12 M 74 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose 0.15 M 75 Slight improvement with Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts Sugars / Saccharides Sucrose 0.18 M 79 Slight improvement w:tn Sugars. Needs further assay optimization Sugars / Saccharides Sucrose 0.21 M 73 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Sucrose 0.2.4 M 82. Slight improvement witfT Sugars. Needs further assay optimization Sugars / Saccharides Sucrose 0.27 M 79 blight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Sucrose 0.3 M 85 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose 0.2 M 76 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Betaine 0.2 M 1- 0.125 M 83 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Betaine 0.2 M +0.25 M 80 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Betaine 0.2 M + 0.5 M 85 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Calcium Chloride 0.2. M + 1 mM 88 SIighf improvemehf with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Raffinose + Calcium Chloride 0.2 M + 5 mM 77 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Calcium Chloride 0.2M + 10mM 90 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Ectoine 0.2 M + 75 mM 88 Slight i m pfovemehf with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Ectoine 0,2 M + 150 mM 81 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides ■■ Raffinose + Ectoine 0.2M + 300mM 79 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Magnesium Sulfate 0.2. M + 1 mM 90 Slight improvemehf with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + Magnesium Sulfate 0.2. M + 5 mM 91 Sugars. Needs further assay Suga rs / Sa ccha r ides Raffinose + Magnesium Sulfate 0.2 M + 10 mM 87 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + TMANO 0.2 M + 0.125 M 81 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + TMANO 0.2 M + 0.25 M 98 Slight I rhprovemenf with Sugars. Needs further assay optimization Sugars / Saccharides Raffinose + TMANO 0.2 M + 0.5 M 84 Slight improvement with Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Additive CatxsAtmton Recovery Resufts Sugars / Saccharides Sucrose 0.24 M 61 Slight improvement w:tn Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Betaine 0.24 M +0.125 M 87 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Sucrose + Betaine 0.24 Ml + 0.245 M 84 Slight improvement with' Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Betaine 0.2.4 M + 0.5 M 84 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Sucrose + Calcium Chloride 0.24 M + 1 mM 83 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Calcium Chloride 0.24 M + 5 mM 87 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Calcium Chloride 0,24 M 1-10 mM 94 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Ectoine 0.24 M +• 75 mM 84 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Ectoine 0.24 M +150 mM 85 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Ectoine 0.24 M + 300 mM 79 SIighf improvemehf wifh Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Sucrose + Magnesium Sulfate 0.24 M + 1 mM 74 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Magnesium Sulfate 0.24 M + 5 mM 89 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + Magnesium Sulfate 0.24 M + 10 mM 79 STigfif i m pfovemehf with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + TMANO 0.24 M 1- 0.125 M 77 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides ”4 Sucrose + TMANO 0.24 M + 0.245 M 87 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Sucrose + TMANO 0.2.4 M + 0.5 M 79 51 ighf improvemehf with' Sugars. Needs further assay optimization HRP Stabiiizer Components Phenol Red 0.01% 70 No improvement HRP Stabilizer Components Phenol Red 0.03% 71 No improvement HRP Stabilizer Components ”4 Phenol Red 0.05% 70 No improvement HRP Stabilizer Components Sodium Benzoate 0.10% 67 No improvement HRP Stabiiizer Components Sodium Benzoate 0.50% 60 No improvement HRP Stabilizer Components Sodium Benzoate 1% 54 No improvement HRP Stabilizer Components Fisher HRP Sol 5% 71 No improvement HRP Stabilizer Components ”4 Fisher HRP So! 10% 70 No improvement HRP Stabilizer Components Fisher HRP Soi 25% 68 No improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (comp) 5% 83 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (spike) b% 71 improvement in %Hb Recovery Formaldehyde Release.' Bronidox 0.0002% 69 No improvement Formaldehyde Releaser Bronidox 0,0010%. 68 No improvement 2026214068   07 Aug 2026 Compound Additive Additive Recovery Resufts Formakiehyde Reieaser B?on;dox "7 / 3 No improvement Enzyme Inhibitor Methylisothiazolone 0.0002% 71 No Improvement Enzyme Inhibitor Methylisothiazolone 0.0010% 68 No improvement Enzyme Inhibitor Methylisothiazolone 0.0020% 63 No improvement Fiavoniods 7,8 dihydroxyflavone 0.25 mM 51 No improvement Fiavoniods 7,8 dihvdroxyflavone 0.5 mM 39 No improvement Flavoniods 7,8 dihvdroxyflavone 1 mM 38 No improvement Fiavoniods Quercetin 150 uM 45 No improvement Flavoniods Quercetin 300 uM 38 No improvement Fiavoniods Quercetin 600 uM 35 No improvement Fiavoniods Diadzein 25 uM 68 No improvement Flavoniods Diadzein 50 uM 78 No improvement Flavoniods Diadzein 100 uM 68 No improvement Redox / antioxidants Ascorbic Acid 0.5 mM 65 No improvement Redox / a ntioxi da nts Ascorbic Acid" 1 mM 67 No improvement Redox / antioxidants Ascorbic Acid 5 mM 57 No improvement Enzyme Inhibitor Methylisothiazolone 0.00005% 68 No improvement Enzyme Inhibitor Methylisothiazolone 0.0001% 68 No improvement Enzyme Inhibitor Methylisothiazolone 0.0002% 67 No improvement Preservatives Sodium Benzoate 0.03% 69 No improvement Preservatives Sodium Benzoate 0.05% 73 No improvement Preservatives Sodium Benzoate 0.10% 65 No improvement Flavoniods Diadzein 40 mM 66 No improvement Flavoniods Diadzein 50 mM 65 No improvement Flavoniods Diadzein 60 mM 66 No improvement Substrates Resveratrol 5 mM 48 No improvement Substrates Resveratrol 10 mM 40 No improvement Substrates Resveratrol 20 mM 38 No improvement Preservatives Sodium Benzoate 0.04% 67 No improvement Preservatives Sodium Benzoate 0.05% 68 No improvement Preservatives Sodium Benzoate 0.06% 67 No improvement Vitamins Tocopherol 0.10% 71 No improvement Vitamins Tocopherol 1% 73 No improvement Vitamins Tocopherol 10% 65 No improvement Vitamins Vitamin B6 0.5 mM 68 No improvement Vitamins Vitamin B6 1 mM 67 No improvement Vitamins Vitamin B6 2 mM 66 No improvement Vitamins Vitamine B6 in HCI 0.5 mM 68 No improvement Vitamins Vitamine B6 in HCI 1 mM 41 No improvement Vitamins Vitamine B6 in HCI 2 mM 40 No improvement Plasma Fetal Bovine Serum 1% 58 No improvement Plasma Fetal Bovine Serum 10% 63 No improvement Plasma Fetal Bovine Serum 50% 68 No improvement Plasma Gamma Globulins 10 mg / rnL 85 No improvement Plasma Gamma Globulins 25 rng / mL 89 No improvement plasma Gamma Globulins 50 mg / mL 88 No improvement Suga rs / Sa ccha rides Glucose 0.2 M 71 SIighf improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose 0.4 M 72 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Glucose 0.6 M 76 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha rides Glucose 0.8 M 73 Slight i m provemenf wit ri Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery R&sufts Sugars / Saccharides Glucose 1.0 M 79 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose 1.2 M 78 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Glucose 1.4 M 80 Slight improvement with' Sugars. Needs further assay optimization Sugars / Saccharides Glucose 1,6 M 76 blight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose 1.8 M 77 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose 2 M 79 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.1 M •77 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.2 M 74 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.3 M 78 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.4 M 77 SIighf improvemehf with' Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Trehalose 0.5 M 82 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.6 M 82 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.7 M 83 Slight7 i m provemenf with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.8 M 82 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.9 M 85 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 1 M 86 SIighf improvemehf with' Sugars. Needs further assay optimization Osmolytes Betaine 1 M 77 Betaine snows improvement in %Hb Recovery at high concentrations Osmolytes Ectoine 0.3 M 65 No improvement Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 78 Magnesium cation snows improvement in %Hb Recovery around 10 mM Osmolytes Trimethylamine N-oxide (TMANO) 1 M 77 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose 0.6 M 7 A SligfffTrh provemenf with Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts Sugars / Saccharides Glucose + Betaine 0.6 M + 0.125 M 74 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Betaine 0.6 M +0.25 M 71 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Glucose + Betaine 0.6 M + 0.5 M 77 Slight improvemehf with' Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Calcium Chloride 0.6 M + 1 mM 71 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose + Calcium Chloride 0.6 M + 5 mM 73 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Calcium Chloride 0.6 M + 10 mM 84 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Ectoine 0.6 M + 0.075 M 73 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Ectoine 0.6 M +0.15 M 72 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Ectoine 0.6 M + 0.3 M 71 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Magnesium Sulfate 0.6 M + 1 mM 75 SIighf improvemehf with' Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Glucose + Magnesium Sulfate 0.6 M + 5 mM 75 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + Magnesium Sulfate 0.6 M + 10 mM 78 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + TMA.NO 0.6 M + 0.125 M 69 Slight7 i m pfovernerif with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + TMANO 0.6 M + 0.25 M 73 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Glucose + TMANO 0.6 M + 0.5 M 68 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose 0.3 M 72. SIighf improvemehf with' Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Betaine 0.3 M + 0.125 M 73 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Betaine 0.3 M + 0.25 M 77 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Betaine 0.3 M + 0.5 M 81 SligfifTrh pfovernerif with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Calcium Chloride 0.3 M + 1 mM 69 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Calcium Chloride 0.3 M + 5 mM 68 Slight improvement with Sugars. Needs further assay optimization 2026214068   07 Aug 2026 Compound Additive Additive CatxsAtmton Recovery Resufts Sugars / Saccharides Trehaiose -r Caicium Chloride 0.3 M 1-10 mM 83 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Ectoine 0.3 M + 0.075 M 77 Slight improvement with Sugars. Needs further assay optimization Suga rs / Saccha rides Trehalose + Ectoine 0.3 M +0.15 M 72 Slight improvement witfT Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Ectoine 0 3 M + 0.3 M 76 Slight improvement with Sugars. Needs further assay optimization Suga rs / Sa ccha r ides Trehaiose +• Magnesium Sulfate 0.3 M + 1 mM 68 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehalose + Magnesium Sulfate 0.3 M + 5 mM 80 Slight improvement with Sugars. Meeds further assay optimization Sugars / Saccharides Trehalose + Magnesium Sulfate 0.3 M 1-10 mM 76 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehaiose + TMANO 0.3 M + 0.125 M 70 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehaiose + TMANO 0.3 M +0.25 M 79 Slight improvement with Sugars. Needs further assay optimization Sugars / Saccharides Trehaiose + TMANO 0.3 M + 0.5 M 75 SIighf improvement wifn Sugars. Needs further assay optimization Sugars / Saccharides Erlose 1 mM 72 No improvement Sugars / Saccharides Erlose 5 mM 69 No improvement Sugars / Saccharides Erlose 10 mM 76 No improvement Suga rs / Sa ccha r ides Fructose 0.05 M 67 No improvement Sugars / Saccharides Fructose 0,1 M 69 No improvement Sugars / Saccharides Fructose 0.25 M 65 No improvement Sugars / Saccharides Galactose b.i m 71 No improvement Sugars / Saccharides Galactose 0.25 M 68 No improvement Sugars / Saccharides Galactose 0.5 M 76 No improvement Sugars / Saccharides Kestose 1 mM 71 No improvement Sugars / Saccharides Kestose 5 mM 68 No improvement Sugars / Saccharides Kestose 10 mM 76 No improvement Sugars / Saccharides Lactose 0.025 M 66 No Improvement Sugars / Saccharides Lactose 0.05 M 71 No Improvement Suga rs / Sa ccha r ides Lactose 0.1 M 65 No Improvement Sugars / Saccharides Maltose 0.1 M 72 No Improvement Sugars / Saccharides Maltose 0.25 M 69 No Improvement Sugars / Saccharides Md 1 toss 0.5 M 76 No Improvement Sugars / Saccharides Melezitose 10 mM 66 No Improvement Sugars / Saccharides Melezitose 50 mM 71 No Improvement Sugars / Saccharides Melezitose 100 mM 65 No Improvement Sugars / Saccharides Stachyose 1 mM 67 No Improvement Sugars / Saccharides Stachyose 5 mM 69 No Improvement Sugars / Saccharides Stachyose 7 mM 65 No Improvement Formaldehyde Reieaser Diazolidinyl Urea, EDTA, Glycine 1% (325g / L, 90g / L, 40 g / L) 66 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea, EDTA, Glycine 1% (500g / L, 90g / L, 40 g / L) 63 No Improvement Formaldehyde Reieaser Diazolidinyl Urea, Imidazolidinyl Urea, EDTA, Glycine 1% (325 g / L, 500g / L, 90g / L, 40 g / L) 62 No Improvement 2026214068   07 Aug 2026 Compound Additive CarKeritratioe Recovery Resufts Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine 5% (325g / L, 90g / L, 40 g / L) 50 No Improvement Formaldehyde Releaser Imidazolidinyl Urea, EDTA, Glycine 5% (500g / L, 90g / L, 40 g / L) 56 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, imidazolidinyl Urea, EDTA, Glycine 5% (325 g / L, 5OOg / L, 90g / L, 40 g / L) 48 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine 10% / 325g / L, 90g / L, 4'0 g / L) 43 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea, EDTA, Glycine 10% (500g / L, 90g / L, 40 g / L) 45 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, Imidazolidinyl Urea, EDTA, Glycine 10% (325 g / L, 500g / L, 90g / L, 40 g / L) 35 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine 1% (1.25%, 2.05%, 0.16%) 64 No Improvement Formaldehyde Reieaser imidazolidinyl Urea, EDTA, Glycine 1%, (2.10%, 2.05%., 0.16%) 71 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, imidazolidinyl Urea, EDTA, Glycine 1% (1.25%, 2.10%, 2.05%, 0.16%) 67 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine W (1.75% 7.05%, 0.16%) 65 No Improvement Formaldehyde Reieaser imidazolidinyl Urea, EDTA, Glycine 10% (2.10%, 2.05%, 0.16%) 65 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, imidazolidinyl Urea, EDTA, Glycine 10% (1.25%, 2.10%, 2.05%, 0.16%) 64 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine 20% (1.25%, 2.05%, 0.16%) 58 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea, EDTA, Glycine 20% (2,10%, 2.05%, 0.16%) 63 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, Imidazolidinyl Urea, EDTA, Glycine 20% (1.25%, 2.10%, 2.05%, 0.16%) 60 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea 400 g / L 6 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea 200 g / L 20 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea 100 g / L 39 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea 50 g / L 55 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea 25 g / L 58 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea 12.5 g / L 62 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea 400 g / L 14 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea 200 g / L 32 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea 100 g / L 45 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea 50 g / L 55 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea 25 g / L 57 No Improvement Formaldehyde Reieaser imidazolidinyl Urea 12.5 g / L 65 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine 10% (325g / L, 90g / L, 40 g / L) 40 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, EDTA, Glycine 1% (325g / L, 90g / L, 40 g / L) 67 No Improvement Formaldehyde Reieaser imidazolidinyl Urea, EDTA, Glycine 10% (500g / L, 90g / L, 40 g / L) 51 No Improvement Formaldehyde Reieaser Imidazolidinyl Urea, EDTA, Glycine 1% (500g / L, 90g / L, 40 g / L) 67 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, Imidazolidinyl Urea, EDTA, Glycine 10% ¢325 g / L, 500g / L, 90g / L, 40 g / L) 42 No Improvement Formaldehyde Reieaser Diazoiidinyl Urea, imidazolidinyl Urea, EDTA, Glycine 1% ¢325 g / L, 500g / l, 90g / L, 40 g / L) 64 No Improvement or o 2026214068   07 Aug 2026 c | For n o For o n o n o’ m L Fo’ For For o n o 9 o’ m o’ For For o For o 9 1 3 QJ 3 CU Qi 3 GJ 3 re 3 GJ q; 3 re 3 3 re 3 re 3 re 3 re 3 GJ QJ 3 GJ QJ 3 3 re 3 3 re 3 re 3 GJ 3 re 3 GJ QJ (V of cl d. re. CL re re” re” re" CL re. cl re, CL re CL re re” re” re” d. re CL re d. re CL re 31 CD re" 31 re Q. re re re’ Q. re re’ 'n. re re" re 31 re re 31 re re’ Q. re re’ 31 re re” re" re” re 31 re re’ 31 re re’ 31 re Q 22 0) tD 23 re 27 re 27 re re 23 re 22 re 23 re 22 re .22 re 22 re ,22 re 23 re re3 23 re re 23 re 22 re .22 re 22 re ,22 re 22 re re3 22 re re 23 re : easer easer leaser leaser ------------------1 re re re leaser leaser leaser leaser leaser re re re leaser easer re re re leaser leaser leaser leaser leaser re re t / i re leaser easer leaser leaser re re re leaser leaser Cfc & g pH 6.5 (RT) azoniaadamantane chloride Urea + Urea, cn' LG 6 GJ 0? □J Glycine, pH 6.5 (RT) azoniaadamantane chloride Urea + l-(ci5-3-Chloroallyl)-3,5,7-triaza-l- 35 azoniaadamantane chloride Urea, pH 6.5 CL r p 6 re d y 53 pH 7.5 (RT) Diazolidinyl Urea + Ammonium Chloride, Diazolidinyl Urea + Urea, pH 7.5 (RT) Diazolidinyl Urea + Glycine, pH 7.5 (RT) p re' I'M 0 d. c: re re i Ui 35 pH 7.0 (RT) Diazolidinyl Urea + Ammonium Chloride, Diazolidinyl Urea + Urea, pH / .0 (RT) Diazolidinyl Urea + Glycine, pH 7.0 (RT) 9 re' o re re b 35 pH6.5(RT) Diazolidinyl Urea -t Ammonium Chloride, Diazolidinyl Urea + Urea, pH 6.5 (Ri) Diazolidinyl Urea + Glycine, pH 6.5 (RT) re' M Q re re p Ui 33 Chloride, pH 7.5 (RT) Imidazolidinyl Urea + Ammonium Imidazolidinyl Urea + Urea, pH 7.5 (RT) imidazolidinyl Urea + Glycine, pH 7.5 (RT) imidazolidinyl Urea, pH 7.5 (RT) Chloride, pH 7.0 (RT) imidazolidinyl Urea + Ammonium Imidazolidinyl Urea + Urea, pH 7.0 (RT) imidazolidinyl Urea + Glycine, pH / .0 (RT) imidazolidinyl Urea, pH 7.0 (RT) Chloride, pH 6.5 (RT) imidazolidinyl Urea + Ammonium imidazolidinyl Urea + Urea, pH 6.5 (RT) imidazolidinyl Urea + Glycine, pH 6.5 (RT) imidazolidinyl Urea, pH 6.5 (RT) & p p p p p o o o p p p o p o p o p p p p Lp O'- o O' o Lp cN Lp O'- -p cN o O' Lp O' Lp O-- a -p co o o O 1 + p p Lp C' + p o + p Lp O' p p p Cp O'- o O o - o p p p o'. p p O 'a? p o p L3 S' o” Lp o'- o? Lp O'- O'-- o' ci'-- Lp O' Lp O'- Lp O' o' O'-- Lp o'- Lp O'- Z i : 1 g X Ui (X' U2 CD Ui ‘D C p cn Ct Cl C Ci .{s C CH Ui CD Cl CD NJ CD Cl ci p s Ci Ci C .p^ cn C Cl Ui CD 3 CD s Ci Ci O O o c 22 o O O O O C c 22 o 22 o o 2: o O C 22 O 22 o ■0 3 -0 3 ‘O 3 3 3 -0 3 3 <5 3 3 3 3 "C 3 -0 1 3 25 1 3 3 3 3 3 -0 Os®? C < re 3 07 ovement ■ovement ■ovement •ovement ■ovement ■ovement •ovement ■ovement •ovement •ovement ■ovement ■ovement •ovement 'ovement ■ovement 'ovement ■ovement •ovement •ovement 'ovement ■ovement ■ovement 'ovement ■ovement 'ovement ■ovement & (ft APPENDIX B 2026214068   07 Aug 2026 Compound Additive Additive Catxetitmton Recovery Resufts Formaldehyde Releaser l-(cis-3-Chloroa iiyl)-3,5,7-triaza-l-azoniaadamantane chloride Urea + Ammonium Chloride, pH 6.5 (RT) 0.1% +0.1% 59 No Improvement Formaldehyde Releaser l-(cis-3-Chloroa 1 lyl)-^,5,7-tnaza-l-azoniaadamantane chloride Urea, pH 7.0 (RTj 0.1% 56 No Improvement Formaldehyde Releaser l-(cis-3-Chloroa 1 lyl) -3,5,7-triaza-l-azoniaadamantane chloride Urea + Glycine, pH 7.0 (RT) 0.1% +0.1% 59 No Improvement Formaldehyde Releaser l-(cis-3-Chloroa lly 1)-3,5,7-triaza-l-azoniaadamantane chloride Urea + Urea, pH 7.0 (RT) 0.1% +0.1% 57 No Improvement Formaldehyde Reieaser l-(cis-3-Chloroaiiyl)-3,5,7-triaza-l-azoniaadamantane chloride Urea + Ammonium Chloride, pH 7.0 (RT) 0.1% +0.1% 59 No Improvement Formaldehyde Reieaser I-{cis-J-Urifbroallyfj-3,5,7-fnaza-I-azoniaadamantane chloride Urea, pH 7.5 (RT) 0.1% 58 No Improvement Formaldehyde Reieaser l-(cis-3-Chloroa 1 lyl)-3,5,7-triaza-l-azoniaadamantane chloride Urea + Glycine, pH 7.5 (RT) 0.1%. +0.1% 57 No Improvement Formaldehyde Reieaser l-(cis-3-Chloroailyl)-3,5,7-triaza-l-azoniaadamantane chloride Urea + Urea, pH 7 5 (RT) 0.1% +0.1%, 55 No Improvement Formaldehyde Reieaser l-(cis-3-Chloroa 1 lyl) -3,5,7-triaza-l-azoniaadamantane chloride Urea + Ammonium Chloride, pH 7.5 (RT) 0.1% +0.1% 58 No Improvement Formaldehyde Reieaser Diazo!idinyl Urea + Glycine, pH 7,5 (50C.) 0.1%+ 0.1% 62 No Improvement Formaldehyde Reieaser Diazolidiny 1 Urea + Glycine, pH 7.5 (50C) + methylisothiazolone 0.1% + 0.1% + 0.2% 42 No Improvement Formaldehyde Reieaser DiazolidinyI Urea + Glycine, pH 7.5 (50C) + methylisothiazolone 0.1%+ 0.1%+ 0.1% 43 No Improvement Formaldehyde Reieaser Diazolidinyi Urea + Glycine, pH 7.5 (50C) + methylisothiazolone 0.1% + 0.1% + 0.05% 41 No Improvement Formaldehyde Reieaser Diazolidiny! Urea + Glycine, pH 7.5 (50C) 0.3%+ 0.3% 64 No Improvement Formaldehyde Reieaser Diazolidiny! Urea + Glycine, pH 7.5 (50C) + methylisothiazolone 0.3% + 0.3% + 0.6% 42 No Improvement Formaldehyde Reieaser Diazolidiny! Urea + Glycine, pH 7.5 (50C) + methyiisothiazolone 0.3%+ 0.3%+ 0 3% 33 No Improvement Formaldehyde Reieaser Diazolidiny! Urea + Glycine, pH 7.5 (50C) + methyiisothiazolone 0.3%+ 0.3%+ 0.15% 35 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 6,5 0.002%. + 0.002%. 72 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 7.5 0.002% + 0.002% 68 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 8.5 0.020% + 0.002% 70 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 6.5 0.001% + 0.002% 71 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 7.5 0.001% + 0.002% 67 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 8.5 0.001% 4 0.002% 68 No Improvement Formaldehyde Reieaser Bronopol + ATA, pH 6.5 0.004% + 0.002% 71 No Improvement 2026214068   07 Aug 2026 Compound Additive Additive Recovery Resufts Formakiehyde Reieaser Bronopol a ATA,. pH 7.5 0.004% a 0.002% 71 No Improvement Formaldehyde Releaser Bronopol t ATA. pH 8.5 0.004% a 0.002% 72 No Improvement Formaldehyde Reieaser Bronopol + Methvlisothiazolone, pH 6.5 0.002% a- 0.002% 63 No Improvement Formaldehyde Release;' Bronopol + Methvlisothiazolone, pH 7.5 0.002% a 0.002% 65 No Improvement Formaldehyde Releaser Bronopol + Methylisothiazolone, pH 8.5 0.002% a-0.002% 59 No Improvement Formaldehyde Releaser Bronopol + Methylisothiazolone, pH 6.5 0.001% + 0.002% 62 No Improvement Formaldehyde Releaser Bronopol a Methylisothiazolone, pH 7,5 0,001%. a 0,002%. 65 No Improvement Formaldehyde Releaser Bronopol + Methylisothiazolone, pH 8.5 0.001% a 0.002% 64 No Improvement Formaldehyde Releaser Bronopol + Methvlisothiazolone, pH 6.5 0.004% a 0.002% 65 No Improvement Formaldehyde Releaser Bronopol + Methvlisothiazolone, pH 7.5 0.004% a 0,002% 66 No Improvement Formaldehyde Releaser Bronopol + Methylisothiazolone, pH 8.5 0.004% + 0.002% 65 No Improvement Formaldehyde Releaser Diazolidiny 1 Urea a- ATA, pH 6.5 0.002% 4- 0.002% 71 No Improvement Formaldehyde Releaser DiazolidinyI Urea + ATA, pH 7.5 0.002% a 0.002% 66 No Improvement Formaldehyde Releaser Diazolidiny! Urea + ATA, pH 8.5 0.002% a- 0.002% 69 No Improvement Formaldehyde Releaser Diazo!idiny 1 Urea + ATA, pH 6,5 0.001% a 0,002% 69 No Improvement Formaldehyde Releaser Diazolidiny! Urea + ATA, pH 7.5 0.001% a-0.002% 65 No Improvement Formaldehyde Releaser Diazolidiny! Urea a- ATA, pH 8.5 0.001% 4 0.002% 73 No Improvement Formaldehyde Releaser Diazolidiny! Urea a- ATA, pH 6.5 0,004%. a 0,002%. 69 No Improvement Formaldehyde Releaser Diazolidiny! Urea a- ATA, pH 7.5 0.004% a 0.002% 65 No Improvement Formaldehyde Releaser Diazolidiny! Urea + ATA, pH 8.5 0.004% a 0.002% 70 No Improvement Formaldehyde Releaser Diazolidinyl Urea + Methylisothiazolone, pH 6.5 0.002% a 0.002% 58 No Improvement Formaldehyde Releaser Diazolid:nyl Urea a Methylisothiazolone, pH 7.5 0.002% a 0.002% 58 No Improvement Formaldehyde Releaser Diazolidiny! Urea + Methylisothiazolone, pH 8.5 0.002% 4 0.002% 60 No Improvement Formaldehyde Releaser Diazolidiny! Urea + Methylisothiazolone, pH 6.5 0.001% a 0.002% 62 No Improvement Formaldehyde Releaser Diazolidiny! Urea a Methylisothiazolone, pH 7.5 0.001% a 0.002% 62 No Improvement Formaldehyde Releaser Diazoiidinyl Urea + Methylisothiazolone, pH 8.5 0.001% a 0,002% 58 No Improvement Formaldehyde Releaser Diazoiidinyl Urea + Methylisothiazolone, pH 6.5 0.004% a 0.002% 60 No Improvement Formaldehyde Releaser DiazoHdinyi Urea a Methylisothiazolone, pH 7.5 0.004% 4- 0.002% 62 No Improvement Formaldehyde Releaser Diazoiidinyl Urea + Methylisothiazolone, pH 8 5 0,004%. a 0,002%. 59 No Improvement Aldehydes Formaldehyde 0.10% 58 No Improvement Aldehydes Formaldehyde 0.50% 54 No Improvement Aldehydes Formaldehyde 1% 25 No Improvement Aldehydes Formaldehyde 2% 5 No Improvement Aldehydes Formaldehyde 3% 4 No Improvement Aldehydes Formaldehyde 4% 4 No Improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts Aldehydes Formaldehyde 5% b No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 93 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15% + 10 mM 99 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 10% 84 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 85 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 20% 84 improvement in %Hb Recovery HRP Stabilizer Components retai Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300 in 10 mM MOPS, pH 6.3 10% (1%, 0.02%, 0.02%, 0.002%) 53 No Improvement HRP Stabilizer Components Fefai BovineSerum, Bronidox, ivietnyi isothiazolone, Proclin 300 in 10 mM MOPS, pH 6.3 15% (1%, 0.02%, 0.02%, 0.002%) 51 No improvement HRP Stabilizer Components tetai tsovine serum, aromoox, ivietnyi isothiazolone, Proclin 300 in 10 mM MOPS, pH 6.3 20% (1%, 0.02%, 0.02%, 0,002%) c4 No Improvement HP.P Stabilizer Components Fetal Bovine Serum, Bromoox, Methyl isothiazolone, Proclin 300 in 10 mM MOPS, pH 6.3 10% (1%, 0.02%, 0.02%, 0.002%) 59 No Improvement HRP Stabilizer Components Fetal Bovine Serum,'Bronidox, MefriyT isothiazolone, Proclin 300 in 10 mM MOPS, pH 6.3 15% (1%, 0.02%, 0.02%, 0.002%) 61 No improvement HRP Stabilizer Components retai Bovine serum, bromaox, ivietnyi isothiazolone, Proclin 300 in 10 mM MOPS, pH 6 3 20% (1%, 0.02%, 0.02%, 0.002%) 49 No Improvement HRP Stabilizer Components Fetal Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300 Calcium Chloride in 10 mM MOPS, pH 6.3 10%, (1%, 0,02%, 0,02%, 0.002%, 10 mM) 54 No improvement HRP Stabilizer Components Fetal Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300 Calcium Chloride in 10 mM MOPS, pH 6.3 15% (1%, 0.02%, 0.02%, 0.002%, 10 mM) 55 No 1 improvement HRP Stabilizer Components Fetal Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300 Calcium Chloride in 10 mM MOPS, pH 6.3 20% (1%, 0.02%, 0.02%, 0.002%, 10 mM) 56 No improvement HRP Stabilizer Components Fetal Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300, Magnesium Sulfate in 10 mM MOPS, pH 6.3 10% (1%, 0.02%, 0.02%, 0.002%, 10 mM) No Improvement HRP Stabilizer Components Fetal Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300, Magnesium Sulfate in 10 mM MOPS, pH 6.3 15% (1%, 0.02%, 0.02%, 0.002%, 10 mM) No improvement HRP Stabilizer Components Fetal Bovine Serum, Bronidox, Methyl isothiazolone, Proclin 300, Magnesium Sulfate in 10 mM MOPS, pH 6.3 20% (1%, 0.02%, 0.02%, 0.002%, 10 mM) 53 No improvement Substrates diphosphoglycerate 1 mM 68 No Improvement Metal Chelators Phytic. Acid" 1%, 33 No Improvement Formaldehyde Reieaser Diazolidinyl Urea, EDT.A, Glycine in 10 mM MOPS, pH 7.5 1% (325g / L, 90g / L, 40 g / L) 63 No Improvement Formaldehyde Reieaser Diazolidinyl Urea, EDTA, Glycinein 10 mM MOPS, pH 7.5+ Diphosphoglycerate 1% (325g / L, 90g / L, 40 g / L, 1 mM) 37 No improvement 2026214068   07 Aug 2026 Compound Additive Additive CatxsAirsdtm Recovery Resufts Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 7.5 + Phytic Acid 1% (325g / L, 90g / L, 40 g / L, 1%) 38 No Improvement Formaldehyde Release;' Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 8.5 1% (325g / L, 90g / L, 40 g / L) 34 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycinein 10 mM MOPS, pH 8.5+ Diphosphoglycerate 1% (325g / L, 90g / L, 40 g / L, 1 mM) 58 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 8.5 + Phytic Acid 1% (325g / L, 90g / L, 40 g / L, 1%) 57 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 7.5 10% (325g / L, 90g / L, 40 g / L) 65 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycinein 10 mM MOPS, pH 7.5+ Diphosphoglycerate 10% (325g / L, 90g / L, 40 g / L, 1 mM) 39 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 7,5 + Phytic Acid 10% (325g / L, 90g / L, 40 g / L, 1%) 39 No Improvement Formaldehyde Release;' Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 8.5 10% (325g / L, 90g / L, 40 g / L) 51 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycinein 10 mM MOPS, pH 8.5+ Diphosphoglycerate 10% (325g / L, 90g / L, 40 g / L, 1 mM) 70 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 8.5 + Phytic Acid 10% (325g / L, 90g / L, 40 g / L, 1%) / 6 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 7.5 20% (325g / L, 90g / L, 40 g / L) 57 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDT.A, Glycinein 10 mM MOPS, pH 7.5+ Diphosphoglycerate 20% (325g / L, 90g / L, 40 g / L, 1 mM) 52 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 7.5+ Phytic Acid 20% (325g / L, 90g / L, 40 g / L, 1%) 49 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 8 5 20% (325g / L, 90g / L, 40 g / L) 24 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycinein 10 mM MOPS, pH 8.5+ Diphosphoglycerate 20% (325g / L, 90g / L, 40 g / L, 1 mM) 46 No Improvement Formaldehyde Releaser Diazoiidinyl Urea, EDTA, Glycine in 10 mM MOPS, pH 8.5 + Phytic Acid 20% (325g / L, 90g / L, 40 g / L, 1%) 44 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 86 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) (diluted with water) 15% (diluted 4% with water) 82 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 93 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride 10 mM 75 Calcium cation shows improvement in %Hb Recovery at 10 mM HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15% 4 1G m M 90 Improvement in %Hb Recovery Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 75 Magnesium Cation shows improvement in %Hb Recovery around 10 mM 2026214068   07 Aug 2026 Compound Additive Additive Recovery R&sufts HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 82 imp coven": ent in Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102) (diluted with water) 15% (diluted 4% with water) 91 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride (comp) 15% + 10 mM 90 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PM 85R-102) + Calcium Chloride (spike) 15% + 10 mM 102 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate (comp) 15%. + 10 mM 92 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate (spike) 15% 4 10 mM 97 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Cobait Chloride 15% + 10 mM 65 improvement in %1-lb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102)+ Copper Chloride 15%+ 10 mM 77 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Iron (III) Chloride 15% + 10 mM 75 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Nickel Chloride 15% + 10 m M 90 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN S5R-i.0‘2) + Zinc Chloride 15% 4 10 mM 91 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 87 improvement in %Hb Recovery HRP Stabilizer Components HR* Conjugate Stabilizer (PN S5H-102) (diluted with water) 15% (diluted 4% with water) 82 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 100 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15%+ 10 mM 96 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Aluminum Chloride 15% + 10 mM 90 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Lithium Chloride 15% + 10 mM 118 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Sodium Chloride 15% + 10 mM 79 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride anhydrous 0.1 mM 73 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride anhydrous 1 mM 71 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride anhydrous 2 mM •77 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride anhydrous 4 mM 73 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride anhydrous 6 mM 77 UaTcTum cation sfiows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride anhydrous 8 mM 75 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride anhydrous 10 mM 79 caicium cation snows improvement in %Hb Recovery at 10 mM 2026214068   07 Aug 2026 Compound Adddlvs Additive CatxeiAimton Recovery Resufts Polyvalent lons / Metal Salts Calcium Chloride anhydrous 20 mM 76 Calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 0.1 mM 71 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Saits Calcium Chloride dihydrate 1 mM 70 UaTcTum cation sTiows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 2 mM 71 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 4 mM 74 caicium cation snow's improvement in %Hb Recovery at 10 rnM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 6 mM 74 Caicium cation snow's improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 8 mM •77 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 10 rnM 77 calcium cation snows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Calcium Chloride dihydrate 20 mM 73 Caicium cation shows improvement in %Hb Recovery at 10 mM Plasma Human Plasma 1% 96 No improvement. High Background Signals Plasma Human Plasma (no sample) 1% 99 No improvement. High Background Signals Plasma Human Plasma 5% 97 No improvement. High Background Signals Plasma Human Plasma (no sample) 5% 94 No improvement. High Background Signals Plasma Human Plasma 10% 96 No improvement. High Background Signals Plasma Human Plasma (no sample) 10% 98 No improvement. High Background Signals Plasma Human Plasma 25% 98 No improvement. High Background Signals Plasma Human Plasma (no sample) 25% 97 No improvement. High Background Signals Plasma Hurrian Plasma 50% 99 No improvement. High Background Signals Plasma Human Plasma (no sample) 50% 97 Mo improvement. High Background Signals Plasma Human Plasma 0.005% 81 No improvement. High Background Signals Plasma Human Plasma (no sample) 0,005%. 103 No improvement. High Background Signals Plasma Human Plasma 0.005% 77 No improvement. High Background Signals Plasma Human Plasma (no sample) 0.005% 99 No improvement. High Background Signals Plasma Human Plasma 0.01% 81 No improvement. High Background Signals Plasma Human Plasma (no sample) 0.01% 106 No improvement. High Background Signals Plasma Human Plasma 0.01% 80 No improvement. High Background Signals Plasma Human Plasma (no sample) 0.01% 101 No improvement. High Background Signals 2026214068   07 Aug 2026 Compound Additive Cancentmten Recovery Resufts Plasma Human Plasma 0.05% 101 No improvement High Background Signals Plasma Human Plasma (no sample) 0.05% 100 No improvement. High Background Signals Plasma Human Plasma 0.05% 95 No improvement. High Background Signals Plasma Human Plasma (no sample) 0.05% 98 No improvement. High Background Signals Plasma Human Plasma 0.01% 99 No improvement. High Background Signals Plasma Human Plasma (no sample) 0.01% 99 No improvement. High Background Signals Plasma Human Plasma 0.01% 99 No improvement. High Background Signals Plasma Human Plasma (no sample) 0.01% 99 No improvement. High Background Signals Plasma Human Albumin 0.0025 mg / mL 71 No improvement. High Background Signals Plasma Human Albumin (no sample) 0.0025 mg / mL 105 No improvement. High Background Signals Plasma Human Albumin 0.025 mg / mL 90 No improvement. High Background Signals Plasma Human .Albumin (no sample) 0.025 mg / mL 95 No improvement. High Background Signals Plasma Human Albumin 0.25 mg / mL 98 Mo improvement. High Background Signals Plasma Human Albumin (no sample) 0.25 mg / mL 95 No improvement. High Background Signals Plasma Human Albumin 2.5 mg / mL 99 No improvement. High Background Signals Plasma Human Albumin (no sample) 2.5 mg / ml 98 No improvement. High Background Signals Plasma Human Albumin 25 mg / ml 98 No improvement. High Background Signals Plasma Human Albumin (no sample) 25 mg / ml 96 No improvement. High Background Signals Plasma Glycine 0.0002 mg / mL 68 No improvement Plasma Glycine (no sample) 0.0002 mg / mL 88 No improvement Plasma Glycine 0.002 mg / mL 68 No improvement Plasma Glycine (no sample) 0.002 mg / mL 218 No improvement Plasma Glycine 0.02 mg / mL 71 No improvement Plasma Glycine (no sample) 0.02 mg / mL 132. No improvement Plasma Crohn's Fraction ll / lll 0.005 mg / mL 70 No improvement. High Background Signals Plasma Crohn's Fraction ii / lli (no sample) 0 005 mg / mL 106 No improvement. High Background Signals Plasma Crohn's Fraction ll / lll 0.05 mg / mL 80 No improvement. High Background Signals Plasma Crohn's Fraction ii / lli (no sample) 0.05 mg / mL 107 No improvement. High Background Signals Plasma Crohn's Fraction ll / lll 0,5 mg / ml. 99 No improvement. High Background Signals Plasma Crohn's Fraction ll / lll (no sample) 0.5 mg / mL 103 No improvement. High Background Signals Plasma Crohn's Fraction ll / iil 5 mg / mL 100 No improvement. High Background Signals Plasma Crohn's Fraction 11 / 111 (no sample) 5 mg / mL 97 No improvement. High Background Signals Plasma Crohn's Fraction ll / lll 50 mg / mL 97 No improvement. High Background Signals Plasma Crohn's Fraction ii / lli (no sample) 50 mg / ml 109 No improvement. High Background Signals 2026214068   07 Aug 2026 Compound Additive Additive Concentmten Recovery Resufts Plasma Crohn's Fraction IV 0.005 mg / mL 106 No improvement Hign Background Signals Plasma Crohn's Fraction IV (no sample) 0.005 mg / mL 93 No improvement. High Background Signals Plasma Crohn's Fraction IV 0.05 mg / mL 100 No improvement. High Background Signals Plasma Crohn's Fraction IV (no sample) 0.05 mg / mL 88 No improvement. High Background Signals Plasma Crohn's Fraction IV 0.5 mg / mL 98 No improvement. High Background Signals Plasma Crohn's Fraction IV (no sample) 0.5 mg / mL 94 No improvement. High Background Signals Plasma Crohn's Fraction IV 5 mg / mL 96 No improvement. High Background Signals Plasma Crohn's Fraction IV (no sample) 5 mg / mL 97 No improvement. High Background Signals Plasma Crohn's Fraction IV 50 mg / mL 98 No improvement. High Background Signals Plasma Crohn's Fraction IV (no sample) 50 mg / mL 98 No improvement. High Background Signals Plasma HDL 0.0025 mg / mL 69 No improvement Plasma HDL (no sample) 0.0025 mg / mL 128 No improvement Plasma HDL 0.025 mg / mL 69 No improvement Plasma HDL (no sample) 0.025 mg / mL 117 No improvement Plasma HDL 6.25 mg / mL 70 No improvement Plasma HDL (no sample) 0.25 mg / mL 106 No improvement Plasma LDL 0.0025 mg / mL 69 No improvement Plasma LDL (no sample) 0.0025 mg / mL 109 No improvement Plasma LDL 0.025 mg / mL 70 No improvement Plasma LDL (no sample) 0.025 mg / mL 93 No improvement Plasma LDL 0.25 mg / mL 67 No improvement Plasma LDL (no sample) 0.25 mg / mL 180 No improvement Plasma p h os p h a t i d y 1 c h o 1 i n e O.Olmg / mL 67 No improvement Pi as ma — phosphatidylcholine (no sample) O.Olmg / mL 80 No improvement Prasrrra phLcpnaLiiJyliJiJliiie 0.1 mg / mL 68 No improvement Plasma phosphatidylcholine (no sample) 0.1 mg / mL 91 No improvement Plasma phosphatidylcholine 1 mg / mL 62 No improvement Plasma phosphatidylcholine (no sample) 1 mg / mL 118 No improvement Plasma Bovine Serum 0.001% 71 No improvement Plasma Bovine Serum (no sample) 0.001% 94 No improvement Plasma Bovine Serum 6.61% 72 No improvement Plasma Bovine Serum (no sample) 0.01% 62 No improvement Plasma Bovine Serum 0.10% 61 No improvement Plasma Bovine Serum (no sample) 0.10% 71 No improvement Plasma Bovine Serum 1% 38 No improvement Plasma Bovine Serum (no sample) 1% 126 No improvement Plasma Lyopholized Human Plasma 0,001%. 71 No improvement. High Background Signals Plasma Lyopholized Human Plasma (no sample) 0.001% 96 No improvement. High Background Signals Plasma Lyopholized Human Plasma 0.01% 79 No improvement. High Background Signals Plasma Lyopholized Human Plasma (no sample) 0.01% 104 No improvement. High Background Signals Plasma Lyopholized Human Plasma 0.10% 93 No improvement. High Background Signals Plasma Lyopholized Human Plasma (no sample) 0.10% 99 No improvement. High Background Signals sjeuSiS punojcbpeg l|§!h 'juaiuaAOjdwi on 86 %OT'O (V09m EZ) eiAiseid uewnH as eujseig s|EU§is punoj8>|3Eg i|8iH piiaiuaAOJdLiii on ZET %T0'0 ■ aidiiJES ou) (V09V1Z6 EZ) emsi'ic uewriH aS Eujsqd S|BU§|S punoj§>peg q§!H NuainaAOjaiui on Z8 %T0'0 (V09t'V6 EZ) ewseid uetunn GS eiuseid s|eu§<s punojrbpeg HiewuAOJdiui 8ET %T00'0 (aid ujes OU) (W9W6 EZ) ewseid uemriH GS Ewse|d s|eu3is punoj§>pBg q§(H puaujaAOjdiui on TZ %T000 {VOO^Vb EZ) eiusEid UEinriH as eiuseid s|eu3is punoj8>peg q§!H 'juaiuaAOJdiUi on 90T %l (aidines OU) (V6S17176 EZ) ewseid UBiunH OS eiuseid s|eu§is punoj8>pBg t|8iH 'jiJswaAOJdLUi on TOT %T (V6£m EZ) euuseid uewnH as eujseid s|Eu8i$ punoj8>psg qSi|-i ■juaiuaAOJdsui on Wl %OT'O ■ aidiiiES ou) (V6St?1z6 EZ) ewsEig UBwnn aS BLUSBid S|BU§|S punoj§>peg y§iH NuaiuaAOjdtui on V6 %OT'O (V6Sn-6 EZ) ewsBjd UBiunn GS ewseid s|eu§js punoj§>j3eg qSiH ’luawsAOJdiUi on 96 %T0'0 (aidiuES ou) (V6St't’6 EZ) euseid ueiunH as eiuseid s|eu3is punoj§>pBg qSi|-f 'luaiijaAOjdiui on T6 %T0'0 EZ) eiusEid uelumh aS eiuseid sjEuSiS punojcbpeg q§!H 'juau.ieAOjdiui on IzTT %T000 (SldlUBS OU) (V65V1Z6 EZ) BLUSBid UBiunH QS eiuseid s|eu§!$ punojaspsg t|8iH pijaiuaAOJdLUi on ZZ %T000 (vest’fre EZ) eiusEid UBUinH as SLUSS id S|BU§|S punoj§>peg q§!H NuaiuaAOjdiui on 66 %l (aidiuES OU) (SSEZZ) ewsB|d UBiunH paiood eiuseid s|eu§<s punojrbpeg qSiH ^ueiuaAOJdiui on V6 %T (SSEZZ) BUJ5B|d UBiunH pa|OOd eiusejd s|eu3is punoj§>pBg q§IH 'luaiijaAOjdiui on DOT %OT'O (aidiues ou) (SSEZZ) BwsBid ueiunH pa|OOd eiuseid s|bu3is punoj8>peg U§iH puaiuaAOjdiui on 86 %OT'O (SSEZZ) eiuse|d UBiunH paioog eiuseid s|eu§!$ punojaspsg t|8iH pijaiuaAOJdLUi on 6TT %T0'0 ■ aidiues ou) Icc^z.} biuseih uewnid paioog SLUSS id s|Eu8i$ punoj8>psg qSi|-i ■juaiuaAOJdsui on 88 %T0'0 ewsEiij ueiunH P^ioo^ BLUSBid s|eu§<s punojrbpeg qSiH HiswBAOJdiU! on T8 %T00'0 (aid IDEs OU) (SSEZZ) BLUSBid ueiunq paioog eiusejd s|eu§js punoj§>j3eg qSiH ‘lUSiusAOJdtUi on %T00'0 ■SSEZZ) suusEid uewnH pa|OOd eiuseid s|bu3is punoj8>peg q§!H puaiuaAOjdiui on 66 %l (aidiues ou) (ZETZZ) eiusEid UBwnn paioog eiuseid sjEuSiS punojcbpeg U§iH 'HiaLueAOjdiui on 86 %T (ZETZZ) eiusEjd ubiuph paioog eiusejd s|Eu8i$ punoj8>psg qSi|-i ■juaiuaAOJdsui on TOT %OT'O (aidiiiES ou) (ZETZZ) siusEld UBiunH paiood BLUSBid S|BU§|S punoj§>peg y§iH NuaiuaAOjdtui on S6 %OT'O (ZETZZ) BUJSB|d ueiunH pajood eiuseid s|eu§js punoj^jpeg q§!H ’lusujSAOJdiui on 86 %T0'0 (aidiues ou) (ZETZZ) BLUSBid ubiuph paiood eiuseid s|eu3is punoj§>pBg q§iH 'luaiijaAOjdiui on 08 %T0'0 (ZETZZ) BWSB|d ueiunH Paiood Eiuseid sjEuSiS punojcbpeg L|§!H piiaLueAOjdiui on 8TT %T000 (aidiues ou) izsizz) eiuseig uetunn paiood eiusejd s|eu§!$ punojaspsg t|8iH pijaiuaAOJdLUi on 69 %T000 (ZETZZ) Bwseid UBiunH paioog eiuseid S|BU§|S punoj§>peg q§!H NuaiuaAOjdiui on 00T %T (aidiues ou) Biuseid ueiunH p^Z!|oqdoA^ eiuseid s|eu§<s punojrbpeg qSiH jueiusACudtu; on 56 %T euiSE|d ueiunH paz!|oqdo / q eiusejd 2026214068   07 Aug 2026 8 XIGN3ddV 2026214068   07 Aug 2026 Compound Additive Catxetitmton Recovery Resufts Plasma Sb*                                  ....... sample) 0.10% 102 No improvement Hign Background Signals Plasma SD Human Plasma (23 94460A) 1% 96 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A) (no sample) 1% 104 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A), spun 2x 0.001% 73 No improvement. High Background Signals Plasma 5D‘ Ruman'FIasma (23 54450A), spun 2x (no sample) 0.001% 101 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A), spun 2x 0.01% 84 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A), spun 2x (no sample) 0.01% 89 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A), spun 2x 0.10% 99 No improvement. High Background Signals Plasma SD Human Plasma (2.3 94460A), spun 2x (no sample) 0.10% 95 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A), spun 2x 1%, 101 No improvement. High Background Signals Plasma SD Human Plasma (23 94460A), spun zx (no sample) 1% 105 No improvement. High Background Signals Plasma SD Human Plasma (23 94464A) 0.001% 77 No improvement. High Background Signals Plasma 5D‘ Hu ma n Plasma (23 94454A) {no sample) 0.001% 91 Mo improvement. High Background Signals Plasma SD Human Plasma (23 94464A) 0.01% 91 No improvement. High Background Signals Plasma SDHuman Plasma (23944WA)’(no sample) 0.01% 114 No improvement. High Background Signals Plasma SD Human Plasma (23 94464A) 0.10% 99 No improvement. High Background Signals Plasma .......SbHumanTHas^....... sample) 0.10% 101 No improvement. High Background Signals Plasma SD Human Plasma (23 94464A) 1% 99 No improvement. High Background Signals Plasma SD Human Plasma (23 94464A) (no sample) 1% 103 No improvement. High Background Signals Plasma SD Human Plasma (23 95813A) 0.001% 71 No improvement. High Background Signals Plasma SD Human Plasma (23 95813A) (no sample) 0.001% 106 No improvement. High Background Signals Plasma SD Human Plasma (23 95813A) 0.01% 75 No improvement. High Background Signals Plasma SD Human Piasma (23 95813A) (no sample) 0.01% 96 No improvement. High Background Signals Plasma SD Human Piasma (23 95813A.) 0.10% 103 No improvement. High Background Signals Plasma SD Human Plasma (23 95813A) (no sample) 0.10% 103 No improvement. High Background Signals Plasma SD Human Plasma (23 95813A) 1%, 100 No improvement. High Background Signals Plasma SD Human Plasma (23 95813A) (no sample) 1% 105 No improvement. High Background Signals Plasma SD Human Plasma (23 95819A) 0.001% 72 No improvement. High Background Signals Plasma SD Human Plasma (23 95819A) {no sampie) 0.001% 109 No improvement. High Background Signals Plasma SD Human Piasma (23 95819A) 0.01% 77 No improvement. High Background Signals Plasma SD Human Plasma (23 95819A) (no sample) 0.01% 136 No improvement. High Background Signals 2026214068   07 Aug 2026 Compound Additive Additive CotxsAtmton Recovery Resufts Plasma SD Human Plasma (23 95819A) 0.10% 103 No improvement Hign Background Signals Plasma SD Human Plasma (23 958T9A) {no sample) 0,10% 107 No improvement. High Background Signals Plasma SD Human Plasma (23 95819A.) 1% 101 No improvement. High Background Signals Plasma SD Human Plasma (23 95819A) (no sample) 1% 108 No improvement. High Background Signals Non-covalent Hb interactions MOPS + BSA 15% (10 mM + 1%) 76 No improvement Polyvalent lons / Metal Salts Calcium Chloride 10 mM 75 Calcium cation shows improvement in %Hb Recovery at 10 mM Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 73 Magnesium Cation shows improvement in %Hb Recovery around 10 mM HRP Stabilizer Components HRP Conjugate Stabilizer (PN ab!71537) 15% 76 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) + Caicium Chloride 15% + 10 mM 87 improvement in %Hb Recovery HRP Stabilizer Components MRP Conjugate Skbiiizer (PN a b 171.5 3 7) + Magnesium Sulfate 15% + 10 mM 89 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) (diluted with water) 15% + 4% 76 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 15% 75 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + Calcium Chloride 15% 4 1G mM 87 improvement in %Hb Recovery HRP Stabilizer Components HRP ‘Conjugate Stabilizer {PN SZOZJ + Magnesium Sulfate 15% + 10 mM 92 improvement in %1-ib Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) (diluted with water) 15% + 4% 77 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 77 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 92 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15% + 10 mM 95 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) (diluted with water) 15% + 4% 77 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride 10 mM 71 No improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 79 improvement in %Hb Recovery HRP Stabilizer Components "W'CSHKigateStabilj / e^ Caicium Chloride 15% + 10 mM 85 improvement in %Hb Recovery Suga rs / Sa ccha r ides Galaturonic Acid 1% 37 No improvement Suga rs / Saccha rides Galaturonic Acid 1% + 10 mM 41 No improvement Suga rs / Sa ccha rides Galaturonic Acid 0.10% 75 No improvement Suga rs / Sa ccha rides Galaturonic Acid 0,1% + 10 mM 72 No improvement Suga rs / Sa ccha rides Galaturonic Acid 0.01% 69 No improvement Sugars / Saccharides Galaturonic Acid 0.01% + 10 mM 73 No improvement Suga rs / Sa ccha rides Polygalacturonic Acid 0.20% 70 No improvement Sugars / Saccharides Poiygaiacturonic Acid + Calcium Chloride 0.2% +10 mM 58 No improvement Sugars / Saccharides Polygalacturonic Acid 0.10% 70 No improvement Sugars / Saccharides Polygalacturonic Acid + Calcium Chloride 0.1% + 10 mM 83 improvement in %1-ib Recovery Sugars / Saccharides Polygalacturonic Acid 0.05% 68 No improvement Sugars / Saccharides Poiygaiacturonic Acid + Calcium Chloride 0.05%, + 10 mM 75 improvement in %Hb Recovery Sugars / Saccharides Polygalacturonic Acid 0.01% 69 No improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CotxsAtmton Recovery Resufts Suga rs / Sa ccha r ides Polygalacturonic Acid + Calcium Chloride 0.01%+ 10 mM 74 imp coven": ent in Recovery Polyvalent lons / Metal Salts Calcium Chloride 10 mM 72 improvement in %Hb Recovery Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 76 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 10 mM + 10 mM 72 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 78 Improvement in %1-lb Recovery HP.P Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Caicium Chloride 15% + 10 mM 83 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15% + 10 mM 78 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCi2 + MgSO4 15% + 10 mM + 10 mM 82 improvement in %>Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) 15% 72 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537j + Calcium Chloride 15% + 10 mM 78 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 3 / )171537,1 + Magnesium Sulfate 15% 4 10 m M 76 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Buffers- CaCI2 + MgSO4 15% + 10 mM + 10 mM 78 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 15% 71 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + Calcium: Chloride 15% + 10 mM 77 improvement in %Hb Recovery HRP Stabilizer Components HRPConjugate Stabilizer (PN SZC / j + Magnesium Sulfate 15% + 10 m M 79 improvement in %Hb Recovery HRP Stabilizer Components MR!5 Conjugate Stabilizer (PN 5202) -r CaCI2 + MgSO4 15% + 10 mM + 10 mM 80 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride 10 mM 82 improvement in %Hb Recovery Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 89 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 10 mM + 10 mM 81 improvement in %>Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 10 mM + 5 mM 78 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 5 mM + 10 mM 90 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride + Magnesium Sulfate 5 mM + 5 mM 87 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 80 improvement in %>Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Caicium Chloride 15%. + 10 mM 87 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Magnesium Sulfate 15% + 10 m M 92 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 8SR-102) + CaCI2 + MgSO4 15%, + 10 mM + 10 mM 87 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCi2 + MgSO4 15% + 10 rnM + 5 mM 89 improvement in %>Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 + MgSO4 15% + 5 mM + 10 mM 90 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 + MgSO4 15% + 5 mM + 5 mM 89 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 15% 78 improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive              Additive Catxetitmton Recovery Resufts HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) ■+ Calcium Chloride 15% + 10 mM 89 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + Magnesium Sulfate 15%+ 10 mM 86 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + CaCI2 + MgSO4 15% + 10 mM + 10 mM 90 improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN SZ02) + CaCI2 + MgSO4 15% + 10 mM + 5 mM 89 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) ■+ CaCi2 + MgSO4 15% + 5 mM + 10 mM 82 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + CaCI2 + MgSO4 15% + 5 mM + 5 mM 87 improvement in %Hb Recovery pH Adjustments Hb Collection Buffer, pH 6.5 NA 84 improvement in %Hb Recovery Poiyvaient lons / Metal Salts Calcium Chloride 10 mM 79 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 89 improvement in %Hb Recovery Sugars / Saccharides Polygalacturonic Acid + Calcium Chloride 0.01% + 10 mM 83 improvement in %Hb Recovery Sugars / Saccharides Poiygaiacturonic Acid + Calcium Chloride 0.025% + 10 mM 91 improvement in %Hb Recovery Suga rs / Saccha rides Polygalacturonic Acid + Calcium Chloride 0.05%, + 10 mM 86 improvement in %Hb Recovery Sugar s / Saccha rides Polygalacturonic Acid + Calcium Chloride 0.075% + 10 mM 79 improvement in %Hb Recovery Suga rs / Saccha rides Polygalacturonic Acid + Calcium Chloride 0,1% + 10 mM 64 improvement in %Hb Recovery Suga rs / Saccha rides Poiygaiacturonic Acid + Calcium Chloride 0.125% + 10 mM 86 improvement in %Hb Recovery Suga rs / Saccha rides Sodium: Alginate (Medium Viscosity) 0.01% 58 No Improvement Suga rs / Saccha rides Sodium Alginate (Medium Viscosity) + CaCI2 0.01%+ 10 mM 62 No improvement Suga rs / Saccha rides Sodium Alginate (Medium Viscosity) 0.05% 56 No Improvement Suga rs / Saccha rides Sodium Alginate (Medium Viscosity) + CaCI2 0.05%, + 10 mM 58 No Improvement Suga rs / Saccha rides Sodium Alginate (Medium Viscosity) 0.10% 46 No Improvement Sugar s / Saccha rides Sodium Alginate (Medium Viscosity) + CaCI2 0.1% + 10 mM 45 No improvement Suga rs / Saccha r ides Sodium Alginate (Medium Viscosity) 0.20% 29 No Improvement Suga rs / Saccha rides Sodium Alginate (Medium Viscosity) + CaCI2 0.2% + 10 mM No improvement Suga rs / Saccha rides Sodium Alginate (Synthetic) 0.01% 61 No improvement Suga rs / Sa ccha r ides Sodium Alginate (Synthetic) + CaCI2 0.01% + 10 mM 62 No Improvement Suga rs / Sa ccha r ides Sodium Alginate (Synthetic) 0.05% 50 No Improvement Suga rs / Sa ccha rides Sodium Alginate (Synthetic) + CaCI2 0.05%, + 10 mM 57 No Improvement Suga rs / Sa ccha rides Sodium Alginate (Synthetic) 0.10% 57 No improvement Suga rs / Sa ccha rides Sodium Alginate (Synthetic) + CaCi2 0.1%+ 10 mM 52 No Improvement Sugars / Saccharides Sodium Alginate (Synthetic) 0.20% 56 No Improvement Suga rs / Sa ccha rides Sodium Alginate (Synthetic) + CaCi2 0.2% + 10 mM 50 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 78 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 80 improvement in %Hb Recovery Sugars / Saccharides Sodium Alginate (Low Viscosity) 0.01% 59 No Improvement Sugars / Saccharides Sodium Alginate (Low Viscosity) + CaCI2 0.01% +10 mM 61 No improvement Sugars / Saccharides Sodium Alginate (Low Viscosity) 0.05% 53 No improvement Sugars / Saccharides Sodium Alginate (Low Viscosity) + CaCI2 0 05% + 10 mM 59 No Improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery Resufts Suga rs / Saccha rides Sodium Alginate (Low Viscosity) 0.t0% 54 No Improvement Suga rs / Sa ccha r ides Sodium Alginate (Low Viscosity) + CaCi2 0.1% a-10 mM 64 No Improvement Suga rs / Sa ccha rides Sodium Alginate (Low Viscosity) 0.20% 48 No Improvement Suga rs / Sa ccha rides Sodium Alginate (Low Viscosity) + CaCI2 0.2% + 10 mM 54 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 79 Improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102) + Calcium Chloride 15% + 10 mM 81 improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102) 100% 78, Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 100% + 10 mM 83 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + MgSO4 100%, ■+ 10 mM 78 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 + MgSO4 100% + 10 mM + 10 mM 82 improvement in %Hb Recovery HRP Stabi!izer Components HRP Conjugate Stabilizer (PN abl71537j 100% 72. Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) + CaCI2 100% + 10 mM 78 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 3bl71537) + MgSO4 100%, ■+ 10 mM 76 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN abl71537) + CaCI2 + MgSO4 100% + 10 mM + 10 mM 78 improvement in %Hb Recovery HRP Stabi!izer Components HRP Conjugate Stabilizer (PN SZ02) 100% 71 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) ■+ CaCI2 100% + 10 mM 77 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + MgSO4 100%, ■+ 10 mM 79 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) + CaCI2 + MgSO4 100% + 10 mM + 10 mM 80 improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride, pH 6.5 10 mM 72 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102), pH 6,5 15% 87 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCI2, pH 6.5 15%+ 10 mM 86 Improvement in %Hb Recovery HRP Stabilizer Components BSA 10% 66 No Improvement HRP Stabilizer Components BSA, pH 6.5 10% 69 No Improvement HRP Stabilizer Components BSA + HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 10% + 15% 80 Improvement in %Hb Recovery HRP Stabilizer Components BSA 5% 67 No Improvement HRP Stabilizer Components BSA, pH 6.5 5% 67 No Improvement HRP Stabilizer Components BSA + HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 5% + 15% 7 A Improvement in %Hb Recovery HRP Stabilizer Components BSA 1% 56 No Improvement HRP Stabilizer Components BSA, pH 6.5 1% 60 No Improvement HRP Stabilizer Components BSA + HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 1% + 15% 75 Improvement in %Hb Recovery HRP Stabilizer Components BSA. 0.50% 50 No Improvement HRP Stabilizer Components BSA, pH 6.5 0.50% 56 No Improvement HRP Stabilizer Components BSA + HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 0.5%+ 15% 80 Improvement in %Hb Recovery HRP Stabilizer Components BSA. 0% 47 No Improvement HRP Stabilizer Components BSA, pH 6.5 0% 51 No Improvement HRP Stabilizer Components BSA + HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 0% + 15%. 83 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX 1 uM 68 No Improvement 2026214068   07 Aug 2026 Compound Additive CotHSirtrstitm Recovery Resufts Protoporphyrin Protoporphyrin iX 10 uM 70 No Improvement Protoporphyrin Protoporphyrin IX 100 uM 66 No Improvement Protoporphyrin Protoporphyrin IX Zinc 1 uM 96 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 10 uM 77 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 100 uM 73 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX + Zinc Chloride 1uM+luM 92 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX + Zinc Chloride 10 uM + 10 uM 87 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX + Zinc Chloride 100 uM + 100 uM 83 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX + Iron (II) Chloride luM+luM 71 No improvement Protoporphyrin Protoporphyrin IX + iron (II) Chloride 10 uM v 10 uM 65 No Improvement Protoporphyrin Protoporphyrin IX + iron (II) Chloride 100 uM + 100 uM 71 No Improvement Protoporphyrin Protoporphyrin IX + Iron (III) Chloride 1uM+luM 65 No Improvement Protoporphyrin Protoporphyrin IX + Iron (III) Chloride 10 uM + 10 uM 66 No Improvement Protoporphyrin Protoporphyrin IX + Iron (III) Chloride 100 uM + 100 uM 60 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 0.15 70 No Improvement Protoporphyrin Protoporphyrin IX 0.001 uM 54 No Improvement Protoporphyrin Protoporphyrin IX 0.01 uM 50 No Improvement Protoporphyrin Protoporphyrin IX 0.1 uM 50 No improvement Protoporphyrin Protoporphyrin IX 1 uM 74 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 0.001 uM 51 No Improvement Protoporphyrin Protoporphyrin IX Zinc 0.01 uM 73 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 88 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 1 uM 66 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX + Zinc Chloride 0.001 uM + 0.001 uM 59 No Improvement Protoporphyrin Protoporphyrin IX + Zinc Chloride 0.01 uM + 0.01 uM 51 No Improvement Protoporphyrin Protoporphyrin IX + Zinc Chloride 0.1 uM + 0,1 uM 57 No Improvement Protoporphyrin Protoporphyrin IX + Zinc Chloride 1uM+luM 53 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 66 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 15% 93 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX 0.1 uM 68 No improvement Protoporphyrin Protoporphyrin IX 1 uM 69 No improvement Protoporphyrin Protoporphyrin IX 10 uM 63 No Improvement Protoporphyrin Protoporphyrin iX Copper 0.1 uM 70 No Improvement Protoporphyrin Protoporphyrin IX Copper 1 uM 67 No Improvement Protoporphyrin Protoporphyrin IX Copper 10 uM 66 No Improvement Protoporphyrin Protoporphyrin IX Magnesium 0.1 uM 65 No Improvement Protoporphyrin Protoporphyrin IX Magnesium 1 uM 69 No Improvement Protoporphyrin Protoporphyrin IX Magnesium 10 uM 73 No Improvement Protoporphyrin Protoporphyrin IX Tin 0.1 uM 69 No Improvement Protoporphyrin Protoporphyrin IX Tin 1 uM 66 No improvement Protoporphyrin Protoporphyrin IX Tin 10 uM 66 No improvement Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 83 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 1 uM 90 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 10 uM 90 Improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Adduce Additive CatxsAtmton Recovery Resufts HRP Stabilizer Components HR? Conjugate Stabilizer (PN S5R-102), pH 6.5 15% 89 Improvement in %Hb Recovery Protoporphyrin Hemin, pH 6.5 0,0003% 101 Improvement in %Hb Recovery Protoporphyrin Hemin + BSA 1.5 mM + 1.5 mM 80 improvement in %Hb Recovery Protoporphyrin Hemin + BSA 22.5 mM + 22.5 mM 88 Improvement in %Hb Recovery Protoporphyrin Hemin + BSA 3 mM + 1.5 mM 81 Improvement in %Hb Recovery Protoporphyrin Hemin + BSA 45 mM + 22.5 mM 92 Improvement in %Hb Recovery Protoporphyrin Hemin + BSA 4.5 mM + 1.5 mM 85 improvement in %Hb Recovery Protoporphyrin Hemin + BSA 67.5 mM + 22.5 mM 88 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Tin + BSA 1.5 mM + 1.5 mM 69 No Improvement Protoporphyrin Protoporphyrin IX Tin + BSA 22.5 mM + 22.5 mM 69 No Improvement Protoporphyrin Protoporphyrin IX Tin + BSA 3 mM + 1.5 mM 68 No Improvement Protoporphyrin Protoporphyrin IX Tin + BSA 45 mM + 22.5 rnM 70 No Improvement Protoporphyrin Protoporphyrin IX Tin + BSA 4.5 mM + 1,5 mM 71 No Improvement Protoporphyrin Protoporphyrin IX Tin + BSA 67.5 mM + 22.5 mM 70 No Improvement Protoporphyrin Protoporphyrin IX Zinc +• BSA 1.5 mM + 1.5 mM 88 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc + BSA 22.5 mM + 2.2.5 mM 86 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc + BSA 3 mM +• 1.5 mM 90 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc + BSA 45 mM + 22.5 mM 87 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc +• BSA 4.5 mM + 1.5 mM 82 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc + BSA 67.5 mM + 2.2.5 mM 78 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX + BSA 1.5 mM + 1.5 mM 66 No Improvement Protoporphyrin Protoporphyrin IX + BSA, 22.5 mM + 22.5 mM 63 No Improvement Protoporphyrin Protoporphyrin IX +• BSA 3 mM + 1.5 mM 64 No Improvement Protoporphyrin Protoporphyrin IX + BSA 45 mM + 22.5 mM 69 No Improvement Protoporphyrin Protoporphyrin IX + BSA 4.5 mM + 1,5 mM 66 No Improvement Protoporphyrin Protoporphyrin IX + BSA 67.5 mM + 22.5 mM 65 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02), pH 6.5 15% 95 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX 0.1 uM 67 No Improvement Protoporphyrin Protoporphyrin IX 1 uM 74 No Improvement Protoporphyrin Protoporphyrin IX 10 uM 64 No Improvement Protoporphyrin Protoporphyrin IX Cobalt 0.1 uM 77 No Improvement Protoporphyrin Protoporphyrin IX Cobalt 1 uM 78 Mo Improvement Protoporphyrin Protoporphyrin IX Cobait 10 uM 116 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium 0.1 uM 71 No Improvement Protoporphyrin Protoporphyrin IX Chromium 1 uM 82 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium 10 uM 117 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Mangansese 0.1 uM 74 No Improvement Protoporphyrin Protoporphyrin IX Mangansese 1 uM 72 No Improvement Protoporphyrin Protoporphyrin IX Mangansese 10 uM 68 No Improvement Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 89 improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive CatxsAtmton Recovery R&sufts Protoporphyrin Protoporphyrin IX Zinc 1 uM 95 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 10 uM 93 Improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN SZ02), pH 6.5 15% 91 No Improvement Protoporphyrin Protoporphyrin IX Cobalt 1 uM 75 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt 2.5 uM 70 No Improvement Protoporphyrin Protoporphyrin IX Cobalt 5 uM 90 Improvement in %Hb Recovery Protoporphyrin ■■ Protoporphyrin IX Chromium 1 uM 83 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium 2.5 uM 96 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium 5 uM 93 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Magnesium 10 uM 61 No Improvement Protoporphyrin Protoporphyrin IX Magnesium 25 uM 66 No Improvement Protoporphyrin Protoporphyrin IX Magnesium 50 uM 76 No Improvement Protoporphyrin Protoporphyrin IX Mangansese 0.025 uM 54 No Improvement Protoporphyrin Protoporphyrin IX Mangansese 0.05 uM 69 No Improvement Protoporphyrin Protoporphyrin IX Mangansese 0.1 uM 63 No Improvement Protoporphyrin Hemin, pH 6.5 0.00% 82 improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 15% 91 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt 5 uM 103 Improvement in %Hb Recovery Protoporphyrin ■■ Protoporphyrin IX Chromium 2.5 uM 109 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 82 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc, pH 6.5 0.1 uM 83 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 1 uM 85 Improvement in %Hb Recovery Protoporphyrin ■■ Protoporphyrin IX Zinc, pH 6.5 1 uM 95 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 84 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc, pH 6.5 0.1 uM 82 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 1 uM 89 Improvement in %Hb Recovery Protoporphyrin ■■ Protoporphyrin IX Zinc, pH 6.5 1 uM 103 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 79 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc, pH 6.5 0.1 uM 87 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 1 uM 81 Improvement in %Hb Recovery Protoporphyrin ■■ Protoporphyrin IX Zinc, pH 6.5 1 uM 90 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc 0.1 uM 85 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Zinc, pH 6.5 0.1 uM 77 Improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive Additive CotxsAtmton Recovery Resufts Protoporphyrin Protoporphyrin IX Zinc 1 uM 86 Improvement in %>Hb Recovery Protoporphyrin Protoporphyrin IX Zinc, pH 6.5 1 uM 82 Improvement in %Hb Recovery Polyvalent lons / Metal Salts Calcium Chloride 10 mM 71 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 79 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) + CaCI2 15% + 10 m M 85 No Improvement Saccharides / Sugars Galaturonic Acid 1% No Improvement Saccharides / Sugars Galaturonic Acid + CaCI2 1%+ 10 mM 41 No Improvement Saccharides / Sugars Galaturonic Acid 0.10% 75 No Improvement Saccharides / Sugars Galaturonic Acid + CaCI2 0 1%+ 10 mM 72 No Improvement Saccharides / Sugars Galaturonic Acid 0.01% 69 No Improvement Saccharides / Sugars Galaturonic Acid + CaCI2 0.01% + 10 mM 73 No Improvement Saccharides / Sugars Polygalacturonic Acid 0.20% 70 No Improvement Saccharides / Sugars Polygalacturonic Acid + CaCi2 0.2% 4 10 mM 58 No Improvement Saccharides / Sugars Polygalacturonic Acid 0.10% 70 No Improvement Saccharides / Sugars Polygalacturonic Acid + CaCI2 0.1% + 10 mM 83 Improvement in %>Hb Recovery Saccharides / Sugars Polygalacturonic Acid 0.05% 68 No Improvement Saccharides / Sugars Polygalacturonic Acid + CaCi2 0.05% 4-10 mM 75 No Improvement Saccharides / Sugars Polygalacturonic Acid 0.01% 69 No Improvement Sa cc h a ri d es / S ug a rs Polygalacturonic Acid + CaCI2 0.01% + 10 mM 74 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 84 Improvement in %Hb Recovery Protoporphyrin Hemin (DMSO) 0.0003% 88 Improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.0003% 84 improvement in %Hb Recovery Protoporphyrin Hemin (NaHCO3) 0.0003% 90 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (DMSO) 0.15%+ 0.0003% 90 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (H2O:NaOH) 0.15%+ 0.0003% 87 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (NaHCO3) 0.15% + 0.0003% 90 improvement in %Hb Recovery Protoporphyrin BSA + Hemin (DMSO) 0.001% + 0.0001% 85 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (DMSO) 0.01%+ 0.001% 93 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (DMSO) 0.1% + 0.01%, 85 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (H2O:NaOH) 0.001% + 0.0001% 85 improvement in %Hb Recovery Protoporphyrin BSA + Hemin (H2O:NaOH) 0.01% + 0.001% 90 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (H2O:NaOH) 0.1%+ 0.01% 83 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (NaHCO3) 0.001%, + 0.0001% 85 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin (NaHCO3) 0.01%+ 0.001% 91 improvement in %Hb Recovery Protoporphyrin BSA + Hemin (NaHCOS) 0.1% + 0,01% 72 Improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102), pH 6.5 15% 93 Improvement in %>Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 83 Improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive Recovery R&sufts Protoporphyrin Hemin (H2O:NaOH) 0.0003% 86 improvement in %Hb Recovery Protoporphyrin Hemin (H2O;NaOH) 0 00005% 82 Improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.00009% 87 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.00018% 86 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.00037% 85 improvement in %Hb Recovery Protoporphyrin Hemin (H2O;NaOH) 0 00075% 88 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.00015% 87 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.003% 90 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH), pH 6.5 0.00005% 90 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH), pH 6,5 0 00009% 88 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH), pH 6.5 0.00018% 92 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOHi, pH 6.5 0.00037% 95 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH), pH 6.5 0.00075% 91 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH), pH 6.5 0 00015% 94 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH), pH 6.5 0.003% 89 improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer <PN 85R-102) 15% 95 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.0003% 90 improvement in %Hb Recovery Alternate Hb CB Formulation Hb CB 10% BSA NA 75 NA Alternate Hb CB Formulation Hb CB 5% BSA NA 73 NA Alternate Hb CB Formulation Hb CB 1%, BSA NA 66 NA Alternate Hb CB Formulation Hb CB 0% BSA NA 53 NA Protoporphyrin Hemin + Hb CB 10% BSA 0 00003% 75 improvement in %Hb Recovery Protoporphyrin Hemin + Hb CB 5% BSA 0.00003% 80 improvement in %Hb Recovery Protoporphyrin Hemin Hb CB 1%. BSA 0.00003% 74 improvement in %Hb Recovery Protoporphyrin Hemin + Hb CB 0% BSA 0.00003% 65 No improvement Protoporphyrin Hemin + Hb CB 10% BSA 0.0003% 88 improvement in %Hb Recovery Protoporphyrin Hemin + Hb CB 5% BSA 0.0003% 90 improvement in %Hb Recovery Protoporphyrin Hemin + Hb CB 1% BSA 0.0003% 86 improvement in %Hb Recovery Protoporphyrin Hemin + Hb CB 0% BSA 0.0003% 71 No Improvement Protoporphyrin Hemin + Hb CB 10% BSA 0.003% 84 improvement in %Hb Recovery Protoporphyrin Hemin -t Hb CB 5% BS.A 0.003% 85 improvement in %Hb Recovery Protoporphyrin Hemin Hb CB 1%. BSA 0.003% 68 No Improvement 2026214068   07 Aug 2026 Compound Additive Cancsnimten Recovery R&sufts Protoporphyrin Hemin - Hb CFS 0% BSA 0.003% 62 No Improvement HRP Stabilizer Components HRP Conjugate Stabilizer <PN 85R-102) 15% 91 Improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.0003% 87 improvement in %Hb Recovery Protoporphyrin Hemin + CaCI2 0.0003%+ 10 mM 88 improvement in %Hb Recovery Protoporphyrin Hemin + MgSO4 0.0003% + 10 mM 88 Improvement in %Hb Recovery Protoporphyrin Hemin + FeCI2 0.0003% + 4.8 uM 89 improvement in %Hb Recovery Protoporphyrin Hemin + FeCi2 0.0003% + 9.6 uM 87 improvement in %Hb Recovery Protoporphyrin Hemin + FeCI2 0.0003% + 2.4 uM 88 improvement in %Hb Recovery Protoporphyrin Hemin + FeCI3 0.0003% + 4.8 uM 91 Improvement in %Hb Recovery Protoporphyrin Hemin + FeCI3 0.0003% + 9.6 uM 91 improvement in %Hb Recovery Protoporphyrin Hemin + FeCI3 0.0003% + 2.4 uM 90 improvement in %Hb Recovery Protoporphyrin BSA + Hemin 1% + 0.0003% 90 improvement in %Hb Recovery Protoporphyrin BSA + Hemin + CaC!2 1% + 0.0003% + 10 mM 90 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin + MgSO4 1% -i- 0.0003% + 10 mM 90 improvement in %Hb Recovery Protoporphyrin BSA + Hemin + FeCI2 1% + 0.0003% + 4.8 uM 87 improvement in %Hb Recovery Protoporphyrin BSA + Hemin + FeCI2 1% + 0.0003% + 9.6 uM 90 improvement in %Hb Recovery Protoporphyrin BSA + Hemin + FeCI2 1% + 0.0003% + 2.4 uM 91 Improvement in %Hb Recovery Protoporphyrin BSA + Hemin + FeCI3 1% + 0.0003% + 4.8 uM 90 improvement in %Hb Recovery Protoporphyrin BSA + Hemin + FeCI3 1% + 0.0003% + 9.6 uM 89 improvement in %Hb Recovery Protoporphyrin BSA + Hemin + FeCI3 1% + 0.0003% + 2.4 uM 88 improvement in %Hb Recovery HRP Stabilizer Components HRP Conjugate Stabilizer (PN 85R-102) 15% 94 Improvement in %Hb Recovery Protoporphyrin Hemin. pH 6.4 0.0003% 88 improvement in %Hb Recovery Protoporphyrin Hemin, pH 6.5 0.0003% 88 improvement in %Hb Recovery Protoporphyrin Hemin, pH 6.6 0.0003% 91 improvement in %Hb Recovery Protoporphyrin Hemin, pH 6.7 0.0003% 88 Improvement in %Hb Recovery Protoporphyrin Hemin, pH 6.8 0.0003% 89 improvement in %Hb Recovery Protoporphyrin Hemin, pH 6.9 0.0003% 85 improvement in %Hb Recovery Protoporphyrin Hemin, pH 7.0 0.0003% 87 improvement in %Hb Recovery Protoporphyrin Hemin, pH 7.1 0.0003% 88 Improvement in %Hb Recovery Protoporphyrin Hemin, pH 7.2 0.0003% 83 improvement in %Hb Recovery Protoporphyrin Hemin, pH 7.3 0.0003% 81 improvement in %Hb Recovery 2026214068   07 Aug 2026 Compound Additive Catxetitmton Recovery Resufts Protoporphyrin Hemin.. pH 7.4 0.0003% 81 Improvement in %Hb Recovery Protoporphyrin Hemin, pH 7.5 0,0003%. 79 Improvement in %Hb Recovery HRP Stabiiizer Components HRP Conjugate Stabilizer (PN SZ02), pH 6.5 15% 93 improvement in %Hb Recovery Protoporphyrin Hemin (H2O:NaOH) 0.0003% 95 Improvement in %Hb Recovery Alternate Hb CB Formulation BSA 22.5 uM 80 Improvement in %Hb Recovery Protoporphyrin Hemin 4.5 uM 92 Improvement in %Hb Recovery Protoporphyrin Hemin + Sodium Azide 4.5 uM +0.0072% 92 improvement in %Hb Recovery Protoporphyrin Hemin + Sodium Azide 4.5 uM + 0.014% 92 Improvement in %Hb Recovery Protoporphyrin Hemin + Sodium Azide 4.5 uM + 0.029% 92 Improvement in %Hb Recovery Alternate Hb CB Formulation Sodium Azide 0 0072% 7? No Improvement Alternate Hb CB Formulation Sodium Azide 0.014% 72 No Improvement Alternate Hb CB Formulation Sodium Azide 0.029% 73 No Improvement Protoporphyrin Hemin + BSA 4.5 uM + 22.5 uM 92 Improvement in %Hb Recovery Protoporphyrin Hemin + BSA + Sodium Azide 4.5 uM + 22.5 uM + 0,0072%. 92 improvement in %Hb Recovery Protoporphyrin Hemin + BSA + Sodium Azide 4.5 uM + 22.5 uM 4 0.014% 89 improvement in %Hb Recovery Protoporphyrin Hemin + BSA + Sodium Azide 4.5 uM + 22.5 uM + 0.029% 93 Improvement in %Hb Recovery Protoporphyrin Hemin 4.8 uM 81 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium 2.5 uM 105 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 1) 1.25 uM 76 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 1) 2,5 uM 85 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 1) 5 uM 103 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 1) 6.5 uM 102 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 2) 1.25 uM 75 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 2) 2,5 uM 89 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 2) 5 uM 97 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 2) 6.5 uM 108 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 3) 1.25 uM 71 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 3) 2,5 uM 83 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 3) 5 uM 103 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt (Vendor 3) 6.5 uM 112 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Gallium 1.25 uM 68 No Improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery R&sufts Protoporphyrin Protoporphyrin IX Gallium 2.5 uM 69 No Improvement Protoporphyrin Protoporphyrin IX Gallium 5 uM 71 No Improvement Protoporphyrin Protoporphyrin IX Gallium 6.5 uM 70 No Improvement Protoporphyrin Protoporphyrin IX Cobalt 5 uM 87 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 1) 1.25 uM 91 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 1) 2.5 uM 78 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 1) 5 uM 92 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 1) 6.5 uM 104 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 2) 1.25 uM 11 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 2) 2.5 uM 71 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor 2) 5 uM 82 improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium (Vendor?.) 6.5 uM 91 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Cobalt 5 uM 84 Improvement in %Hb Recovery Protoporphyrin Protoporphyrin IX Chromium 2.5 uM 91 Improvement in %Hb Recovery Protoporphyrin Hemin, Bovine 2.4 uM 92 improvement in %Hb Recovery Protoporphyrin Hemin, Bovine 4.8 uM 89 Improvement in %Hb Recovery Protoporphyrin Hemin, Bovine 9.6 uM 91 Improvement in %Hb Recovery Protoporphyrin Hematin 2.4 uM 87 Improvement in %Hb Recovery Protoporphyrin Hematin 3.6 uM 84 improvement in %Hb Recovery Protoporphyrin Hematin 4.8 uM 89 Improvement in %Hb Recovery Protoporphyrin Hematin 6 uM 89 Improvement in %Hb Recovery Protoporphyrin Hematin 7.2 uM 87 Improvement in %Hb Recovery Protoporphyrin Hematin 8.4 uM 92 improvement in %Hb Recovery Protoporphyrin Hematin 9 6 uM 90 Improvement in %Hb Recovery Protoporphyrin Hemin, Porcine (Vendor 1) 2.4 uM 91 Improvement in %Hb Recovery Protoporphyrin Hemin, Porcine (Vendor 1) 4.8 uM 104 Improvement in %Hb Recovery Protoporphyrin Hemin, Porcine (Vendor 1) 9.6 uM 92 improvement in %Hb Recovery Protoporphyrin Hemin, Porcine (Vendor 2) 2 4 uM 86 Improvement in %Hb Recovery Protoporphyrin Hemin, Porcine (Vendor 2) 4.8 uM 90 Improvement in %Hb Recovery Protoporphyrin Hemin, Porcine (Vendor 2) 9.6 uM 92 Improvement in %Hb Recovery Non covalent Hb interactions Polyvinylpyrrolidone (10,000 Da) 1% 68 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (10,000 Da) 0.50% 65 No Improvement 2026214068   07 Aug 2026 Compound Adddivs Additive CatxsAtmton Recovery R&sufts Non covalent Hb interactions Polyvinylpyrrolidone (10..000 Da) 0.10% 70 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (10,000 Da) 0.05% 67 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (10,000 Da) + CaCI2 0.1%+ 10 mM 77 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (40,000 Da) 1% 68 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (40,000 Da) 0.50% 67 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (40,000 Da) 0.10% 65 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (40,000 Da) 0.05% 68 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone ¢40,000 Da) + CaCI2 0.1%+ 10 mM 75 No Improvement Non covalent Hb interactions Propylene Glycol 1% 67 No Improvement Non covalent Hb interactions Propylene Glycol 0.50% 65 No Improvement Non covalent Hb interactions Propylene Glycol 0.10% 69 No Improvement Protoporphyrin Bilirubin 0.00003% 70 No Improvement Protoporphyrin Bilirubin 0.00030% 68 No Improvement Protoporphyrin Bilirubin 0.00300% 68 No Improvement Protoporphyrin Bilirubin 0.03000% 66 No Improvement Protoporphyrin Biliverdin 0.00003% 68 No Improvement Protoporphyrin Biliverdin 0.00030% 66 No Improvement Protoporphyrin Biliverdin 0.00300% 67 No Improvement Protoporphyrin Biliverdin 0.03000% 60 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (350,000 Da) 0.10% 71 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (350,000 Da) 0.50% 69 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (350,000 Da) 1% 77 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (350,000 Da) 2% 54 No Improvement Non covalent Hb interactions Polyvinylpyrrolidone (350,000 Da) + CaCi2 0.5% + 10 mM 76 No Improvement 2026214068   07 Aug 2026 Compound Cfessification PreservativeswiUvtHTol groups Preservatives with thiol groups groups Preser^ groups Redox / antioxidants Redox / antioxidants Redox / antioxidants Redox / antioxidants Metal Chelators Metal Chelators Crosslinkers STmO’^ (cyanide) Similar to Drabkin's Reagent (cyanide) (cyanide) (cyanide) NorncovalentTiE interactions interactions Redox / antioxidants .....Redox / antioxidants........... Redox / antioxidants Redox / ant^^ Formaidehyde~Refeaser Formaldehyde Releaser Metal Chelators Metal Chelators Preserwiit^^ groups Additive Ad®ve Hb Collection Buffer ■ No additive Polymedco Buffer - No Sodium Thiosulfate Sodium Thiosulfate Sodium Thiosulfate Sodium Thiosulfate (±)-a-Lipoic acid (±)-a-Lipoic acid (±)-a-Lipoic acid (±)-a-Lipoic acid Ethylenediamine-N.N'-disuccinic acid (EDDS) disuccinic acid (EDDS) PdTJacryiicacid] Poly (acrylic acid) Imidazole Imidazole Hydroxylamine Hydroxylamine Dextran Sulphate Dextran Sulphate mPEG2K-Lipoic Acid Urea Urea knjdazoHdFnynJrea Imidazolidinyl Urea TJjNHS-fUarB'oxym'eth glutamic acid tetrasodiumn salt (GLDA) glutamic acid tetrasodiumn salt (GLDA) Cysteine Results NA NA 70 80 Current Buffer Formulation 0.02% 0.01% 0.005% 0.0025% 0.0126% 0.0063% 0.0032% 0.0016% 1 mM 10 mM __ 1 mM 0.25 mM 0.5 mM 0.25 mM 0.5 mM 0.2.5 mM 0.5 mM OlmM 0.5 mM 50 mM 100 mM ”TmM~ 10 mM 1 mM 10 mM 1 mM 52. 59 62 65 64 69 69 70 68 58 66 56 68 63 66 66 69 65 67 64 60 63 65 62 65 40 46 No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement ___ No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement ___ No Improvement NoTnprovement No Improvement No Improvement No Improvement No Improvement 2026214068   07 Aug 2026 groups Preservatives with thiol Cysteine 10 mM 36 No Improvement groups Glutathione 1 mM 62 No Improvement groups Preserva^^ Glutathione 3 mM 62 No Improvement groups PreservativeswiUvtHTol groups Preservatives with thiol groups groups Sug^ Sugars / Saccharides Redox / antioxidants Redox7'an¥o¥da"nts .....Metal Chelators MetaTcheiators FormafHeHyBe^eleaser Formaldehyde Releaser Substrates Crosslinkers CrossiTnkers Preser^ groups WeservativeswrEFFtEioT groups Non -covalent Hb interactions NomcovalentTlE’ interactions Substrates .....Substrates..... Metal Chelators Metal Chelators PreservativeswiUvtHTol groups Preservatives with thioi groups Metal Chelators iMetaTchdators Dithiothreitol Dithiothreitol TCEP TCEP Raffinose Raffinose Asco r b i c Acid Ascorbic Acid Deferoxamine Mesylate Sait Deferoxamine Mesylate Salt DiazonHiHyTUrea Diazolidinyl Urea Poly (acrylic acid) Polyfacryiicacid] Cysteine Cysteine Dextran Sulphate Dextran Sulphate Diphosphoglycerate (DPG) DiphosphogTycerate glutamic acid tetrasocliumn salt (GLDA) H^-i^TCar^ glutamic acid tetrasodiumn salt (GLDA) TCEP TCEP EDTA: Disodium Salt EDTA: Disodium Salt EDTA: Disodium 0.1 mM 1 mM 0.1 mM 1 mM Tm¥ 24 mM lOmM 25 mM 1 mM "SniM 1 mM 10 mM 1 mM 0.01 mM 0.001mM 0.01 mM 0.1 mM 0.0025 mM 0.025 mM 076665 m M 0.002 mM" 0.01 mM 0.1 mM 0.005 mM 0.01 mM 5 m M 10 mM 30rlV 63 59 64 70 69 65 38 22 67 58 65 62 68 65 68 68 61 69 66 70 66 70 67 59 28 66 63 59 No Improvement No Improvement No Improvement No Improvement ___ No Improvement NoTnprovement No Improvement No Improvement No Improvement __ No Improvement No Improvement Nolmprovement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement ___ 2026214068   07 Aug 2026 NorvcovalenFHF interactions Non -covalent Hb interactions NomcovalerltTiE’ interactions Sulfonic Acids Sulfonic Acids Sulfonic Adds ___ interactions interactions NorvcovalenFHF interactions Sulfonic Acids Sulfonic Adds Sulfonic Acids Flavoniods .....Flavoniods..... RedoxTantioxidants (TedoT / a^^^^ .....Redox / antioxidants........... RedoxTaHtioxidants Redox7antlo5<ldants OsnioTytes Osmolytes Metal Chelators Metal Chelators __ Formaldehyde Releaser PreservativeTwiHTtRIor groups Preservatives with thiol groups Metal Chelators Metal Chelators Heparin Heparin Heparin dipotassium salt MetFlanedlsuffoniFacH dipotassium salt Methanedisuifonic add dipotassium salt PEG2OOOO PEG20000 PEG20000 Propanedisuifonic acid disodium salt disodium salt disodium salt Quercetin Quercetin Quercetin Uric Acid UncAod Uric Acid AscorbicAdd Ascorbic Acid Betaine Betaine Deferoxamine Mesylate Salt Deferoxamine Mesylate Salt DlazoHHinyTUrea Diazolidinyl Urea Dithiothreitol Dithiothreitol disuccinic acid (EDDS) disuccinic acid (EDDS) 2,5 uM 25 uM 250 uM 0.114 mM 1.14 mM 10.69 mM 0.024 mM 0.24 mM 24 mM 0.4 mM 4 mM 40 mM ”0.6uM 6 uM 60 uM b''6uM .......6 uM....... 60 uM lOuM 100 uM "50uM” 50b~uM 10 uM 100 uM ibuM lOOuM 1 uM 10 uM 10 uM 100 uM 68 65 66 66 67 66 66 68 62 64 66 66 64 67 59 67 65 65 65 56 63 63 59 68 62 65 61 66 64 No improvement No improvement No improvement No improvement No Improvement No improvement No Improvement No Improvement No Improvement No Improvement No improvement No Improvement Nolmprovement No Improvement No improvement No Improvement No Improvement T^oTnprovement No Improvement NoTnprovement No Improvement No Improvement No Improvement No Improvement No Improvement No improvement No Improvement No Improvement 2026214068   07 Aug 2026 Wes^ groups Preservatives with thiol Glutathione 10 uM 65 No Improvement groups (cyanide) Glutathione Hydroxylamine 100 uM 2.5 uM 56 63 No Improvement No Improvement milar to Drabkin s Reagent (cyanide) liTiarloTraBIcinTReagent (cyanide) nilar to Drabkin's Reagent (cyanide) Formaldehyde Releaser Hydroxylamine Imidazole 25 uM 2.5 uM 63 No Improvement No Improvement Sugar^TSaccharides Osmolytes Osmolytes Redox7antioxTdarits Redo^^ Metal Chelators Metal Chelators Imidazole rhWaTdTidThVnJrea Imidazolidinyi Urea mPEG2K-Lipo(c Acid mPEG2K-Lipoic Acid Raffinose Raffinose Trimethylamine N-oxide (TMANO) ‘nTrimetHyianm (TMANO) Urea glutamic acid tetrasodiumn salt (GLDA) Iminodisuccinic acid 25 uM "lOuM" 100 uM lOuM 100 uM "TuM” ”50uM” 500 uM 5 mM 500 uM 5 mM 10 mM 10 mM groups Preservatives’ with’tliioT groups Preservatives with thiol groups interactions FJonTcovi^^ interactions NomcovalentlTC interactions Substrates Substrates Substrates Metal Chelators Metal Chelators Cysteine Cysteine Cysteine Dextran Sulphate Dextran Sulphate Dextran Sulphate Diphosphoglycerate (DPG) Diphosphoglycerate (DPG) Diphosphoglycerate (DPG) N.N-is-'Carboxymethyi)-:..-glutamic acid tetrasodiumn salt (GLDA) ''N;N-is-('CarT5dxyme'thy^ glutamic acid tetrasodiumn salt (GLDA) 0.1 uM 1 uM 5 uM 0.2 uM 1 uM 2 uM 0.5 uM "5uKF 50 uM 0.1 uM 1 uM 66 68 65 66 65 66 64 64 63 66 62 42 59 65 67 65 69 65 67 67 66 67 65 No Improvement No Improvement ___ No Improvement NoTnprovement No Improvement No Improvement No improvement No Improvement No Improvement No improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement 2026 Metal Chelators N, N - f s - ( Ca rb oxy m ethyl J- E-glutamic acid tetrasodiumn salt (GLDA) 5 uM 68 No Improvement 07 Aug 1     Non-covalent Hb interactions PEG2000 0.033 uM 68 No Improvement Non-covalent Hb interactions PEG2000 0.33 uM 66 No Improvement Non -covalent Hb interactions PEG2000 3.3 uM 66 No Improvement 2026214068 Redox / antioxidants mPEG2K-Lipo:cAcid 0.1 uM 67 No Improvement Redox / antioxidants mPEG2K-Lipoic Acid 1 uM 65 No Improvement Redox / antioxidants mPEG2K-Lipoic Acid 5 uM 66 No Improvement Preservatives with thiol groups TCEP 250 uM 62 No Improvement Preservatives with thiol groups TCEP 500 uM 66 No Improvement Preservatives with thiol groups TCEP 750 uM 68 No Improvement Redox / antioxidants Ascorbic Acid 0.2 uM 69 No Improvement Redox / antioxidants Ascorbic Acid 1 uM 68 No Improvement Redox / antioxidants Ascorbic Acid 5 uM 64 No Improvement Formaldehyde Releaser Diazolidinyl Urea + Imidazolidinyl Urea 10 uM and 10 uM 65 No Improvement Formaldehyde Releaser Diazolidinyl Urea + Imidazolidinyl Urea 100 uM and 100 uM 56 No Improvement Formaldehyde Releaser Diazolidinyl Urea + Imidazolidinyl Urea ImM and 1 mM 61 No Improvement Sugars / Saccharides Glucose 72 uM 67 No Improvement Sugars / Saccharides Glucose 720 uM 69 No Improvement Sugars / Saccharides Glucose 7.2 mM 67 No Improvement Crosslinkers Magnesium Gluconate 1.2 uM 68 No Improvement Crosslinkers Magnesium Gluconate 12 uM 63 No Improvement Crosslinkers Magnesium Gluconate 120 uM 71 No Improvement Enzyme inhibitors Sodium Fluoride 8.5 uM 68 No improvement Enzyme Inhibitors Sodium Fluoride 85 uM 67 No Improvement Enzyme Inhibitors Sodium Fluoride 850 uM 70 No Improvement Enzyme Inhibitors Sodium Fluoride and Glucose 950 uM and 7.2 mM 65 No Improvement Enzyme Inhibitors Sodium Fluoride and Glucose 950 uM and 72 uM 67 No Improvement Enzyme Inhibitors Sodium Fluoride and Glucose 950 uM and 720 uM 65 No Improvement Redox / antioxidants Urea 2.5 uM 70 No Improvement Redox / antioxidants Urea 25 uM 69 No Improvement Redox / antioxidants Urea 250 uM 70 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Acetate + Formaldehyde 10 mM + 0.02% 66 No Improvement 2026 Formaldehyde + Ammonium Salt Complexes Ammonium Acetate + Formaldehyde 100 m M + 0.1% 58 No Improvement 07 Aug I Formaldehyde + Ammonium Salt Complexes Ammonium Acetate + Formaldehyde 767 mM + 1% 37 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Chloride + Formaldehyde 10 m M + 0.02% 63 No Improvement 2026214068 Formaldehyde + Ammonium Salt Complexes Ammonium Chloride + Formaldehyde 50 mM + 0,1% 56 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Chloride + Formaldehyde 95 mM + 1% 17 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Oxalate + Formaldehyde 0.1 m M + 0.2% 65 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Oxalate + Formaldehyde 1 mM + 0.1% 55 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Oxalate + Formaldehyde 4.8 mM + 1% 19 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Sulfate + Formaldehyde 1 mM + 0.02% 65 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Sulfate + Formaldehyde 10 mM + 0.1% 58 No Improvement Formaldehyde + Ammonium Salt Complexes Ammonium Sulfate + Formaldehyde 243 mM + 1% 11 No Improvement Metal Chelators ATMP 0.02 mM 65 No Improvement Metal Chelators ATMP 0.2 mM 67 No Improvement Metal Chelators ATMP 2 mM 42 No Improvement Crosslinkers Cysteamine 0.016 mM 66 No Improvement Crosslinkers Cysteamine 0.16 mM 62 No Improvement Crosslinkers Cysteamine 1.6 mM 45 No improvement Formaldehyde + Ammonium Salt Complexes Hexamethylenetetramine 14 mM 63 No Improvement Formaldehyde + Ammonium Salt Complexes Hexamethylenetetramine 140 mM 55 No Improvement Formaldehyde + Ammonium Salt Complexes Hexamethylenetetramine 500 mM 52 No Improvement Osmolytes Betaine 50 mM 55 No Improvement 2026214068   07 Aug 2026 —---------------— Osmolyte: Osmolytes Osmolytes Metal Chelators Metal Chelators Metal Chelators Flavoniods Flavoniods Flavoniods Crosslinkers Crosslinkers Crosslinkers Flavoniods TiavonTod?” Flavoniods ____ Substrates "Substrates” Flavoniods Flavoniods" Flavoniods Crosslinkers Crosslinkers Crosslinkers Substrates Substrates" Substrates “Substrates" Substrates Betaine (TMANO) (TMANO) Trisodium N-(l-Carboxylatoethyl)iminodiac etate Hydrate (MGDA) Trisodium N-(l-Carboxylatoethyl)iminodiac etate Hydrate (MGDA) Trisodium N-(l-Carboxylatoethyl)iminodiac etate Hydrate (MGDA) 7J3clTFiy^ hydrate hydrate 7 / 8 diKydro^^ hydrate ......................Bis(3.5-..................... dibromosalilcyljfumarate dibromosaiilcyijfumarate Bis^S, 5-dibromosalilcyljfumarate Daidzein Daidzein Daidzein ArglnTne Arginine Arginine Chrysin .....Chrysin'"" Chrysin -- dithioproprionimidate dihydrochloride dithioproprionimidate dihydrochloride dithioproprionimidate dihydrochloride Lysine .....Lysine " Lysine Sodium Bicarbonate 96 mM 50 mM 87 mM 0.1 mM 1 mM 10 mM 0.01 mM 0.1 mM 1 mM 5 uM 10 uM 100 uM ~~O / or^ 0.1 mM 1 mM 0.058 mM 0.58 mM 5"8”m'M 0 / 0254mM 0.254mM” 2.54 mM 0.0032 mM 0.032 mM 0.32 mM 0.0687 mM 0.687 mM ™6 / 877nM~ _____ 0.895 mM 49 65 65 69 65 49 60 59 36 65 59 43 66 62 42 66 68 59 67 56 44 67 63 52 67 67 59 71 66 No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement Nolmprovement No Improvement No improvement No Improvement No Improvement No Improvement No Improvement No Improvement No improvement No Improvement No Improvement 2026214068   07 Aug 2026 —,------------------.------------------,------------------.-----------------------a ? i Substrates Sodium Bicarbonate Sugars / Saccharides Sugars / Saccharides Sugars / Saccharides Trehalose Trehalose Trehalose l-Allyl-3-methylimidazolium Ionic Liquid Chloride l-Allyl-3-methylimidazolium Ionic Liquid Chloride l-Aliyl-3-methylimidazolium Ionic Liquid Chloride l-Allyl-3-methylimidazolium Ionic Liquid Chloride + NaBr l-Aliyl-3-methylimidazolium Ionic Liquid Chloride + NaBr l-Aliyl-3-methylimidazolium Ionic Liquid Chloride + NaBr l-Butyl-3- Ionic Liquid methylimidazoium Chloride 8.95 mM 61 No Improvement 0.0174 mM 65 No Improvement 0.174 mM 68 No Improvement 1.74 mM 66 No Improvement 0.01 mM 69 No Improvement No Improvement 1 mM 70 No Improvement 0.01 mM + 0.01 mM 68 No Improvement O.lmM + O.l mM 66 No Improvement 1mM + 1mM No Improvement 0.01 mM 66 No Improvement Ionic Liquid l-Butyl-3-methylimidazoium Chloride 0.1 mM 67 No Improvement Ionic Liquid l-Butyl-3-methylimidazoium Chloride 1 mM 65 No Improvement Ionic Liquid l-Butyl-3-methylimidazoium Chloride + NaBr 0.01 mM + 0.01 mM 66 No Improvement Ionic Liquid l-Butyl-3-methylimidazoium Chloride + NaBr 0.1 mM + 0.1 mM 63 No Improvement Ionic Liquid I-Butyl-3-methylimidazoium Chloride + NaBr 1mM + 1mM 67 No Improvement Osmolytes Ectoine 0.072 mM 61 No Improvement Osmolytes Ectoine 0.718 mM 64 No Improvement Osmolytes Ectoine 5 mM 45 No Improvement Osmolytes Firoin 0.038 mM 68 No Improvement Osmolytes Firoin 0.377 mM 68 No Improvement Osmolytes Firoin 1 mM 69 No Improvement Substrates Arginine 0.0058 mM 69 No Improvement Substrates Arginine 0.058 mM 68 No Improvement Substrates Arginine 0.25 mM 66 No Improvement 2026214068   07 Aug 2026 Flavoniods Flavoniods FiavonTod? Crosslinkers Crosslinkers Crosslinkers (cyanide) (cyanide) sTmTa^ (cyanide) Substrates Substrates Substrates Sugars / Saccharides Sugars / Saccharides SugarsTSaccharides' Chrysin Chrysin Chrysin ...........D'imet'6yT37^.........’ dithioproprionimidate dihydrochloride ..........DTm'etfiyT3,3T-.......... dithioproprionimidate dihydrochloride ...........DTmeffiy^^......... dithioproprionimidate dihydrochloride Histidine Histidine Histidine Sodium Bicarbonate Sodium Bicarbonate Sodium Bicarbonate .....Trehalose..... Trehalose TreHafose HRP Conjugate Stabilizer (PN SZ02) 0.0000254 mM 0.0000254 mM _____ 0.0000032 mM 0.000032 mM 0.00032 mM 0.0028 mM 0.0228 mM 0.228 mM 0.0000895 mM 0.000895 mM 0.00895 mM 1.74 mM 16.76 mM 68 70 69 68 66 71 67 67 67 70 70 No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement No Improvement HRP Stabilizer Components 5% 79 Improvement Polyvalent lons / Metal Salts Calcium Chloride 10 mM 76 Improvement Polyvalent lons / Metal Salts Magnesium Sulfate 10 mM 74 Improvement HRP Stabilization Components HRP Conjugate Stabilizer (PN 85R-102) 15% 70 Improvement Osmolytes Betaine 3.5M 78 Improvement HRP Stabilizer Components HRP Conjugate Stabilizer (PN SZ02) 15% 79 Improvement Sugars / Saccharides Sucrose 0.24 M 75 Improvement Sugars / Saccharides Trehalose 0.3 M 77 Improvement Protoporphyrin Hemin, pH 6.5 0.0003% 90 Improvement Protoporphyrin Protoporphyrin IX Zinc, pH 6.5 1 uM 89 Improvement Protoporphyrin Protoporphyrin IX Zinc, pH 7.4 1 uM 75 Improvement Protoporphyrin Protoporphyrin IX Chromium, pH 6.5 2.5 uM 117 Improvement 2026214068   07 Aug 2026 Protoporphyrin Protoporphyrin iX Chromium, pH 7,4 2.5 uM 102 Improvement Protoporphyrin Protoporphyrin IX Cobalt. pH 6.5 5 uM 109 Improvement Protoporphyrin Protoporphyrin IX Cobalt. pH 7.4 5 uM 111 Improvement 2026214068   07 Aug 2026 Compound Classification Compound Combinations Crosslinkers 4arm-PEG2K-Maleimide + Iminothiolane hydrochloride mPEG-Mal, 5K + Iminothiolane hydrochloride Trehlose + Glycine Trehalose + Glycerol Glucose + Glycine Glucose + Glycerol Trehalose + Glucose DEAE-Dextrose + Ethylene Glycol + Calcium Chloride Sucrose + Glucose Sucrose + Trehalose Sucrose + Raffinose Raffinose + Trehalose Raffinose + Glucose Raffinose + Betaine Raffinose + CaCI2 Raffinose + Ectoine Raffinose + MgSO4 Raffinose + TMANO Sucrose + Betaine Sucrose + CaCI2 Sugars / Saccharides Sucrose + Ectoine Sucrose + MgSO4 Sucrose + TMANO Trehalose + Betaine Trehalose + CaCI2 Trehalose + Ectoine Trehalose + MgSO4 Trehalose + TMANO Glucose + Betaine Glucose + CaCI2 Glucose + Ectoine Glucose + MgSO4 Glucose + TMANO Sodium Fluoride + Glucose Polygalacturonic Acid + CaCI2 Galacturonic Acid + CaCI2 Sodium Alginate (synthetic) + CaCI2 Sodium Alginate (Medium Viscosity) + CaCI2 Sodium Alginate (Low Viscosity) + CaCI2 Formaldehyde Releasers Diazolidinyl Urea + Glycine + Formaldehyde Diazolidinyl Urea + Glycine Diazolidinyl Urea + Zinc Imidazolidinyl Urea + Glycine + Formaldehyde Imidazolidinyl Urea + Glycine Imidazolidinyl Urea + Diazolidinyl Urea Protoporphyrin IX + Iron Chloride 2026214068   07 Aug 2026 Protoporphyrins Formaldehyde + Ammonium Salt Complexes Ionic Liquids __________________Hemin + BSA_________________ _____________Protoporphyrin IX Tin + BSA_____________ Protoporphyrin IX Zinc + BSA Protoporphyrin IX + BSA ___________Protoporphyrin IX + Zinc Chloride___________ Hemin + CaCI2 Hemin + MgSO4 Hemin + FeCI2 ___________________Hemin + FeCI3___________________ BSA + Hemin BSA + Hemin + CaCI2 BSA + Hemin + MgSO4 ________________BSA + Hemin + FeCI2________________ BSA + Hemin + FeCI3 Hemin + Sodium Azide Hemin + BSA + Sodium Azide ________Ammonium Chloride + Formaldehyde________ ________Ammonium Oxalate + Formaldehyde________ ________Ammonium Sulfate + Formaldehyde_________ ________Ammonium Acetate + Formaldehyde________ l-Allyl-3-methylimidazolium chloride + Sodium Bromide l-Butyl-3-methylimidazolium chloride + Sodium Bromide ______________Copper Chloride + Sucrose______________ Iron Chloride + Sucrose Polyvalent lons / Metal Salts ____________Nickel Chloride + Sucrose____________ _____________Zinc Chloride + Sucrose_____________ ___________Cobalt Chloride + Sucrose___________ _________Magnesium Sulfate + Sucrose_________ ________Calcium Propoinate + PEG 8000________ Calcium Propoinate + PEG 8000 + Calcium Chloride ________________Calcium + Cobalt________________ ______________Calcium + Copper______________ __________________Calcium + Iron__________________ ____________Calcium + Magnesium____________ _________________Calcium + Nickel_________________ _________________Calcium + Zinc_________________ _______________Cobalt + Copper_______________ __________________Cobalt + Iron__________________ _____________Cobalt + Magnesium_____________ _________________Cobalt + Nickel_________________ __________________Cobalt + Zinc__________________ ____________Copper+ Magnesium____________ ________________Copper + Nickel________________ _________________Copper + Zinc_________________ ______________Iron + Magnesium______________ ____________________Iron + Nickel____________________ Iron + Zinc 2026214068   07 Aug 2026 Stabilizer Components ________________Magnesium + Nickel________________ ________________Magnesium + Zinc________________ _____________Calcium Chloride + PEG 8000_____________ ___________Calcium Chloride + PEG 20000___________ _____________Calcium Chloride + PEG 2000_____________ _______________________Nickel + Zinc______________________ ________________Iron Chloride + EDTA________________ __________________Iron Chloride + IDA__________________ ________________Iron Chloride + GLDA________________ Surmodics HRP Stabilization Buffer + Magnesium Sulfate Surmodics HRP Stabilization Buffer + Calcium Chloride Fitzgerald HRP Conjugate Buffer + Magnesium Sulfate Fitzgerald HRP Conjugate Buffer + Calcium Chloride Fitzgerald HRP Conjugate Buffer + Cobalt Chloride Fitzgerald HRP Conjugate Buffer + Copper Chloride Fitzgerald HRP Conjugate Buffer + Nickel Chloride Fitzgerald HRP Conjugate Buffer + Zinc Chloride Fitzgerald HRP Conjugate Buffer + Iron (III) Chloride Fitzgerald HRP Conjugate Buffer + Aluminum Chloride Fitzgerald HRP Conjugate Buffer + Lithium Chloride Fitzgerald HRP Conjugate Buffer + Sodium Chloride

Claims

1. A composition comprising:(a) a stool sample; and(b) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from:a protoporphyrin;a polyvalent cation;a sugar or polysaccharide and, optionally, a polyvalent cation;an osmolyte; andan HRP stabilization component and, optionally, a polyvalent cation.

2. The composition of claim 1, wherein the stool sample contains hemoglobin.

3. The composition of any prior claim, wherein the stool resuspension solutioncomprises protoporphyrin IX complexed with a multivalent cation.

4. The composition of claim 3, wherein the multivalent cation is Cr3+ or Co3+.

5. The composition of claim 3 or 4, wherein the solution has a pH in the range of pH 6.5to pH 7.4.

6. The composition of any of claims 3-5, wherein the multivalent cation is Cr3+ and the pH of the solution is in the range of pH 6.9 to pH 7.4.

7. The composition of any of claims 3-5, wherein the multivalent cation is Co3+ and the pH of the solution is in the range of pH 6.5 to pH 7.0.

8. The composition of any of claims 3-7, wherein the protoporphyrin IX is at a concentration in the range of 0.5 uM to 10 uM.2026214068   07 Aug 20269. The composition of claim 1, wherein the solution comprises a substituted or unsubstituted polygalacturonic acid and, optionally, a polyvalent cation.

10. The composition of claim 9, wherein the substituted or unsubstituted polygalacturonic acid is at a concentration in the range of 0.005% to 0.5%.

11. The composition of any prior claim, wherein the stool sample is suspended in the solution.

12. The composition of any prior claim, wherein the solution further comprises Tris buffer, bovine serum albumen, polysorbate 20, sodium azide, sodium chloride, ethylenediaminetetraacetic acid, and gentamicin.

13. A method of stabilizing hemoglobin in a stool sample, comprising: combining the stool sample with a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from:a protoporphyrin ;a polyvalent cation;a sugar or polysaccharide and, optionally, a polyvalent cation;an osmolyte; andan HRP stabilization component and, optionally, a polyvalent cation;to produce a suspension; and maintaining the suspension for a period of time.

14. The method of claim 13, wherein the method comprises shipping the suspension to a remote location.

15. The method of any of claims 13-14, wherein the stool resuspension solution comprises protoporphyrin IX complexed with a multivalent cation.

16. A method of analyzing a stool sample, comprising:(a) receiving, from a remote location, a composition comprising:(i.) a stool sample; and2026214068   07 Aug 2026(ii.) a stool resuspension solution comprising one or more hemoglobin stabilization reagents selected from:a protoporphyrin;a polyvalent cation;a sugar or polysaccharide and, optionally, a polyvalent cation;an osmolyte; andan HRP stabilization component and, optionally, a polyvalent cation, wherein the stool sample is suspended in the solution; and(b) measuring the amount of hemoglobin in the composition.

17. The method of claim 16, further comprising measuring the amount of one or more colorectal cancer tumor markers in the sample.

18. The method of any of claims 16-17, wherein the stool resuspension solution comprises protoporphyrin IX complexed with a multivalent cation.

19. A sample collection device comprising:a sample collection container having an open end;a stool resuspension solution that is within the container, comprising one or more hemoglobin stabilization reagents selected from:a protoporphyrin;a polyvalent cation;a sugar or polysaccharide and, optionally, a polyvalent cation;an osmolyte; andan HRP stabilization component and, optionally, a polyvalent cation; anda stool sampling rod comprising a distal beveled tip for scooping and / or scraping a sample of stool,wherein the distal end of the sampling rod is dimensioned to be inserted into the sample collection container and the proximal end of the sampling rod is adapted to connect with the open end of the sample collection container, thereby sealing distal end of the sampling rod within the device.

20. The sample collection device of claim 19, wherein the sample collection container and sampling rod connect via a screw fit.2026214068   07 Aug 202621. The sample collection device of claim 19, wherein the stool resuspension solution comprises protoporphyrin IX complexed with a multivalent cation.