High-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings

Through the high-frequency regeneration and rapid propagation method of tissue culture seedlings, ultrasonic cleaning and different formula media are used to promote the differentiation and rooting of adventitious buds and lateral buds of lantern trees, which solves the problems of limited wild resources of lantern trees and difficulty in rooting with conventional cutting technology, and achieves Efficient seedling production and inheritance of excellent traits.

CN105660397AInactive Publication Date: 2016-06-15HANGZHOU LANDSCAPING
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Patent Information

Application Number
CN201610022606.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2016-01-13
Publication Date
2016-06-15
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The number of wild resources of lantern trees is limited, and conventional cutting techniques are difficult to take root and cannot guarantee the stable inheritance of excellent traits, resulting in limited market development.

Method used

The method of high-frequency regeneration and rapid propagation of lantern tree tissue culture seedlings is adopted, which includes selecting individual plants of wild lantern trees without pests and diseases for ultrasonic cleaning and disinfection treatment, and inoculating them into different formulas of starting, multiplication, seedling strengthening and rooting media. By controlling temperature, Light and humidity promote the differentiation and rooting of adventitious buds and lateral buds, ultimately achieving rapid reproduction of seedlings.

Benefits of technology

It is possible to obtain seedlings with consistent specifications and high quality in a short time, which improves production efficiency and stably maintains the excellent traits of wild resources. The rooting rate is as high as 90% and the transplanting survival rate is over 85%.

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Abstract

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.
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Citation Information

Patent Citations

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