Blood separation method using sampling needle and blood cell analyzer

Abstract

The present application provides a blood separation method for a sampling needle and a blood cell analyzer, the method comprising: collecting a blood sample through a sampling needle, the blood sample being distributed into multiple sections in sequence in the sampling needle; discarding the first section of the blood sample near the needle head in the sampling needle; allocating a first preset ratio of the blood sample near the first section of the blood sample in the sampling needle to a reticulocyte counting section; allocating a second preset ratio of the blood sample located in the middle section of the sampling needle to a white blood cell counting section and a hemoglobin / red blood cell counting section; and allocating a third preset ratio of the blood sample near the end of the sampling needle to a white blood cell classification counting section. The blood separation method for a sampling needle and the blood cell analyzer of the present application can allocate multiple blood sections in the sampling needle to appropriate counting sections, thereby improving the measurement performance of the blood cell analyzer.

Description

Technical Field

[0001] The present application relates to the technical field of blood analysis, and more specifically to a blood separation method using a sampling needle and a blood cell analyzer. Background Art

[0002] Hematology analyzers are used to perform operations such as classification and counting of various cells in the blood, thereby outputting measurement parameters of white blood cells, red blood cells, platelets, reticulocytes, etc. that have clinical guidance significance. For different types of cells, hematology analyzers are usually designed with different blood cell counting parts. The blood sample is drawn through a sample needle and quantitatively distributed to different counting parts. The quantitative blood sample is then reacted with a reagent to obtain a predetermined sample solution. Different blood cell measurement parameter results are obtained by measuring specific parameter information in the sample solution.

[0003] Among them, different counting parts have different measurement principles and interferences, among which the influence of blood samples assigned to different counting parts by sampling needles accounts for a large proportion. Therefore, it is particularly important to provide a blood separation method for sampling needles with better measurement performance. Summary of the invention

[0004] A series of simplified concepts are introduced in the Summary of the Invention section, which will be further described in detail in the Detailed Description of the Invention section. The Summary of the Invention section of this application does not mean to attempt to define the key features and essential technical features of the claimed technical solution, nor does it mean to attempt to determine the scope of protection of the claimed technical solution.

[0005] An embodiment of the present invention provides a blood separation method using a sampling needle, the method comprising:

[0006] Collecting a blood sample through a sampling needle, wherein the blood sample is sequentially distributed into multiple sections in the sampling needle;

[0007] Discarding the first section of the blood sample near the needle tip in the sampling needle;

[0008] distributing a first preset proportion of the blood sample in the sampling needle close to the first blood sample to the reticulocyte counting unit;

[0009] Distribute the blood sample of a second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section;

[0010] A third preset ratio of the blood sample near the end of the sampling needle is distributed to the leukocyte differential counting section.

[0011] In one embodiment, the method of distributing the second preset ratio of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises:

[0012] distributing a fourth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample to the white blood cell counting section;

[0013] A fifth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio of the blood sample is distributed to the hemoglobin / red blood cell counting part.

[0014] In one embodiment, the method of distributing the second preset ratio of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises:

[0015] distributing a fourth preset proportion of the blood sample near the end of the sampling needle in the second preset proportion of the blood sample to the white blood cell counting section;

[0016] A fifth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample is distributed to the hemoglobin / red blood cell counting section.

[0017] In one embodiment, an isolated blood segment is provided between each adjacent segment of blood from the blood sample of the first preset ratio to the blood sample of the fifth preset ratio, and the method further comprises: discarding the isolated blood segment.

[0018] In one embodiment, after dispensing the blood sample of the third preset ratio, the method further comprises:

[0019] The final blood sample near the end of the sampling needle is discarded.

[0020] Another aspect of the present invention provides a blood cell analyzer, comprising: a sampling needle, a control device and a counting unit, wherein the control device is used to control the sampling needle to distribute blood samples to multiple counting units:

[0021] The sampling needle collects a blood sample, and the blood sample is sequentially distributed into multiple sections in the sampling needle;

[0022] Discarding the first section of the blood sample near the needle tip in the sampling needle;

[0023] distributing a first preset proportion of the blood sample in the sampling needle close to the first blood sample to the reticulocyte counting unit;

[0024] Distribute the blood sample of a second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section;

[0025] A third preset ratio of the blood sample near the end of the sampling needle is distributed to the leukocyte differential counting section.

[0026] In one embodiment, the method of distributing the second preset ratio of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises:

[0027] distributing a fourth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample to the white blood cell counting section;

[0028] A fifth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio of the blood sample is distributed to the hemoglobin / red blood cell counting part.

[0029] In one embodiment, the method of distributing the second preset ratio of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises:

[0030] distributing a fourth preset proportion of the blood sample near the end of the sampling needle in the second preset proportion of the blood sample to the white blood cell counting section;

[0031] A fifth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample is distributed to the hemoglobin / red blood cell counting section.

[0032] In one embodiment, an isolated blood segment is provided between each adjacent segment of blood from the first preset ratio blood sample to the fifth preset ratio blood sample, and the control device is further used to control the sampling needle to discard the isolated blood segment.

[0033] In one embodiment, after dispensing the blood sample of the third preset ratio, the control device is further configured to:

[0034] The sampling needle is controlled to discard the terminal blood sample near the end of the sampling needle.

[0035] The blood separation method of the sampling needle and the blood cell analyzer of the embodiment of the present invention allocate the blood sample close to the first section of the blood sample to the reticulocyte counting part that needs to output accurate optical parameters, so as to minimize the adhesion effect of this section of the blood sample in the sample needle and the pipeline; allocate the blood sample close to the end of the sampling needle to the white blood cell classification counting part that does not have high quantitative requirements, so as to reduce the impact caused by the dilution effect of the blood sample; thereby, each blood section in the sampling needle is allocated to a suitable counting part, thereby improving the measurement performance of the blood cell analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] By describing the embodiments of the present application in more detail in conjunction with the accompanying drawings, the above and other purposes, features and advantages of the present application will become more apparent. The accompanying drawings are used to provide a further understanding of the embodiments of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present application and do not constitute a limitation of the present application. In the accompanying drawings, the same reference numerals generally represent the same components or steps.

[0037] Figure 1 A schematic flow chart showing a blood separation method using a sampling needle according to an embodiment of the present invention;

[0038] Figure 2A A schematic diagram showing the distribution of blood segments in a sampling needle according to an embodiment of the present invention is shown;

[0039] Figure 2B A schematic diagram showing the distribution of blood segments in a sampling needle according to another embodiment of the present invention is shown;

[0040] Figure 3 A structural block diagram of a blood cell analyzer according to an embodiment of the present invention is shown. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical solutions and advantages of the present application more obvious, the example embodiments according to the present application will be described in detail with reference to the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application, and it should be understood that the present application is not limited to the example embodiments described herein. Based on the embodiments of the present application described in the present application, all other embodiments obtained by those skilled in the art without creative work should fall within the scope of protection of the present application.

[0042] In the following description, a large number of specific details are provided to provide a more thorough understanding of the present application. However, it is apparent to those skilled in the art that the present application can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present application, some technical features well known in the art are not described.

[0043] It should be understood that the present application can be implemented in different forms and should not be interpreted as being limited to the embodiments set forth herein. On the contrary, providing these embodiments will make the disclosure thorough and complete, and will fully convey the scope of the present application to those skilled in the art.

[0044] The purpose of the terms used herein is only to describe specific embodiments and is not intended to be limiting of the present application. When used herein, the singular forms "one", "an" and "said / the" are also intended to include plural forms, unless the context clearly indicates otherwise. It should also be understood that the terms "consisting of" and / or "comprising", when used in this specification, determine the presence of the features, integers, steps, operations, elements and / or parts, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, parts and / or groups. When used herein, the term "and / or" includes any and all combinations of the relevant listed items.

[0045] In order to thoroughly understand the present application, a detailed structure will be proposed in the following description to illustrate the technical solution proposed by the present application. The optional embodiments of the present application are described in detail as follows, but in addition to these detailed descriptions, the present application may also have other implementation methods.

[0046] Next, first refer to Figure 1 A blood separation method 100 using a sampling needle according to an embodiment of the present application is described. Figure 1 As shown, the blood separation method 100 of the sampling needle may include the following steps:

[0047] In step S110, a blood sample is collected through a sampling needle, and the blood sample is distributed into multiple sections in the sampling needle in sequence;

[0048] In step S120, the first section of the blood sample near the needle tip in the sampling needle is discarded;

[0049] In step S130, a first preset proportion of the blood sample in the sampling needle close to the first blood sample is distributed to the reticulocyte counting unit;

[0050] In step S140, a second preset ratio of the blood sample located in the middle section of the sampling needle is distributed to the white blood cell counting section and the hemoglobin / red blood cell counting section;

[0051] In step S150, a third preset ratio of the blood sample near the end of the sampling needle is distributed to the white blood cell differential counting section.

[0052] The blood separation method 100 of the sampling needle of the embodiment of the present application is used for a blood cell analyzer. The blood cell analyzer includes a plurality of counting parts for analyzing different cells in a blood sample. The counting part may also be referred to as a reaction pool, which is used to provide a reaction site for the blood sample and the reagent to be tested to prepare a sample liquid. The sampling needle collects the blood sample and quantitatively distributes it to different counting parts of the blood cell analyzer, so that the blood sample reacts with the reagent to obtain a predetermined sample liquid, and the blood cell analyzer measures specific parameter information in the sample liquid to obtain different blood cell parameter measurement results.

[0053] The blood cell analyzer includes at least a white blood cell counting unit for measuring white blood cell (WBC) count, a white blood cell classification and counting unit for measuring white blood cell classification (DIFF) and counting, a hemoglobin / red blood cell counting unit for measuring hemoglobin (HGB) / red blood cell (RBC) count, and a reticulocyte counting unit for measuring reticulocyte (RET). After the sampling needle collects the blood sample, the blood is separated into each counting unit in a preset order for corresponding measurement.

[0054] Specifically, in step S110 , a blood sample is first collected through a sampling needle.

[0055] For example, the sampling needle can be fixed on the transmission mechanism. When collecting blood samples, the sampling needle can be moved to the location of the blood sample through the transmission mechanism, and the sampling needle can be moved downward to contact the blood sample. At this time, the sampling needle can use the power provided by the power source connected to its end to absorb a quantitative blood sample and store it inside the sampling needle, completing the blood sample collection action. The power source is also used to provide power for the subsequent sampling needle to discharge the blood sample.

[0056] Generally, the sampling needle is cleaned before use to ensure cleanliness or eliminate carryover contamination from the previous blood sample. Figure 2A , Figure 2B Before this sampling, a diluent 208 is provided in the sampling needle. In order to reduce the possible dilution of the blood sample by the diluent 208, before drawing the blood sample, a section of air is firstly inhaled through the sampling needle, and then the sampling needle is moved to the blood sample position. The power source provides power, and a predetermined amount of blood sample is drawn through the needle at the front end of the sampling needle. At this time, the air isolation between the diluent and the blood sample forms an isolation air column 207.

[0057] The blood sample collected by the sampling needle is distributed into multiple sections in the sampling needle, namely Figure 2A and Figure 2B The blood sample in the sampling needle includes a first blood sample 201 near the needle, a reticulocyte counting blood segment 202, a leukocyte counting blood segment 203, a hemoglobin / red blood cell counting blood segment 204, and a leukocyte differential counting blood segment 205. In some embodiments, the blood sample in the sampling needle also includes a final blood sample 206 near the isolation gas column 207. An isolation blood segment is also provided between each blood sample segment. In some embodiments, other blood segments may also be provided between each of the above blood segments. It is understandable that the blood sample in the sampling needle is continuously distributed, and the different blood segments in the above blood sample are defined according to the function of each blood segment.

[0058] Afterwards, in step S120 , the first blood sample 201 near the needle tip in the sampling needle is discarded.

[0059] Since blood samples are usually stored in a sealed manner, for example, after collecting blood samples from the subject, they are stored in a test tube with a rubber cap. Therefore, the method of collecting blood samples with a sampling needle can include an open mode and a closed mode. The open mode means that the operator opens the rubber cap of the test tube and then loads it, and the sampling needle enters the test tube to absorb the sample. The closed mode means that the rubber cap of the test tube is not opened, and the sampling needle pierces the rubber cap of the test tube and enters the test tube to absorb the blood sample.

[0060] In the closed mode, due to the difference between the internal pressure of the test tube and the external air pressure, when the sampling needle enters and leaves the test tube, the pressure at the needle tip will change stepwise. The sampling needle and the pipeline connected to the sampling needle have a certain elasticity. When the pressure at the needle hole changes stepwise, the blood sample stored in the sampling needle will be displaced to a certain extent, resulting in errors in the quantitative distribution of the blood sample. Therefore, before the blood sample is quantitatively distributed to each counting unit, the embodiment of the present invention first discards the first section of blood sample 201 close to the needle tip to avoid the above-mentioned errors.

[0061] In step S130, a first preset ratio of blood sample in the sampling needle close to the first blood sample 201 is distributed to the reticulocyte counting unit. Figure 2A , Figure 2B The reticulocyte counting blood segment 202 is shown above the first blood sample segment 201. It should be understood that the "first preset ratio" and the "second preset ratio", "third preset ratio", "fourth preset ratio" and "fifth preset ratio" below can be set or adjusted as needed.

[0062] Reticulocytes are transitional cells between late immature erythrocytes and mature erythrocytes, and are slightly larger than mature erythrocytes. When counting reticulocytes, the fluorescence method can be used to count reticulocytes, and different types of cells can be distinguished based on the optical information of the cell particles. Specifically, a fluorescent reagent is reacted with a first preset proportion of a blood sample allocated to the reticulocyte counting unit, the cell particles in the blood sample are labeled, and a sample liquid is obtained. An appropriate amount of the sample liquid is then transported to an optical detection device for light irradiation, and the fluorescence and scattered light generated by each cell particle in the sample liquid are detected. The reticulocyte count value is obtained based on the fluorescence intensity information and scattered light intensity information of each cell particle.

[0063] Since the reticulocyte counting needs to output accurate optical platelet (PLT) parameters, and since PLT is easy to adhere to the sample needle and the pipeline, in order to ensure the accuracy of the optical PLT parameters, the reticulocyte counting blood segment 202 is set at the bottom of each effective blood segment of the sampling needle, close to the first blood sample 201. When the blood sample in the sampling needle is distributed, the first preset proportion of the reticulocyte counting blood segment 202 is first distributed to the reticulocyte counting part, so as to minimize the adhesion effect of the reticulocyte counting blood segment 202 in the sample needle and the pipeline.

[0064] Next, in step S140, a second preset ratio of the blood sample located in the middle section of the sampling needle is distributed to the white blood cell counting section and the hemoglobin / red blood cell counting section.

[0065] Among them, the white blood cell counting unit is used to measure the total number of white blood cells. White blood cell counting can be achieved based on laser irradiation. After the blood sample assigned to the white blood cell counting unit reacts with the reagent to obtain a sample liquid, the sample liquid is irradiated with light in an optical detection device, and each particle in the sample liquid will scatter and emit fluorescence. At this time, the light signal generated by each particle in the sample liquid due to light irradiation is collected. The light signal includes forward scattered light, side scattered light, fluorescence, etc. The forward scattered light signal reflects the size information of the cell, the side scattered light signal reflects the complexity of the internal structure of the cell, and the fluorescence signal reflects the content of substances such as DNA and RNA in the cell that can be dyed with fluorescent dyes. The white blood cell count value can be obtained using these scattered light signals.

[0066] Similarly, in the hemoglobin / red blood cell counting section, the blood sample assigned to the counting section is first reacted with a reagent to obtain a sample liquid, and the sample liquid is irradiated with light in an optical detection device, and the light signal generated by each particle in the sample liquid due to the light irradiation is collected, and the red blood cell count value is obtained according to the light signal. Afterwards, the hemoglobin content of a single red blood cell particle can be determined from the scattered light intensity of the single red blood cell particle, and the count value of the red blood cell particle obtained by statistics and the hemoglobin content of the single red blood cell particle can be used to obtain the average hemoglobin content of the red blood cell particle group.

[0067] The white blood cell counting section and the hemoglobin / red blood cell counting section output main parameters such as white blood cell count and hemoglobin / red blood cell count respectively. Therefore, in the embodiment of the present invention, the white blood cell counting blood segment 203 allocated to the white blood cell counting section and the hemoglobin / red blood cell counting blood segment 204 allocated to the hemoglobin / red blood cell counting section are arranged in the middle section of the sampling needle.

[0068] In one embodiment, referring to Figure 2ASince the white blood cell counting has a high requirement on the incubation reaction time, the blood sample of the fourth preset ratio close to the sampling needle tip in the blood sample of the second preset ratio is first allocated to the white blood cell counting part, and then the blood sample of the fifth preset ratio close to the end of the sampling needle in the blood sample of the second preset ratio is allocated to the hemoglobin / red blood cell counting part; that is, the white blood cell counting blood segment 203 is first allocated to the white blood cell counting segment, which is conducive to allocating the blood sample to the reaction pool of the white blood cell counting segment for incubation reaction earlier, thereby facilitating the overall improvement of the measurement speed; then, the hemoglobin / red blood cell counting blood segment 204 is allocated to the hemoglobin / red blood cell counting segment.

[0069] In another embodiment, referring to Figure 2B When the measurement speed requirement is not high, the fifth preset ratio of the blood sample near the sampling needle tip in the second preset ratio of the blood sample can be first allocated to the hemoglobin / red blood cell counting section, and then the fourth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio of the blood sample can be allocated to the white blood cell counting section; that is, the hemoglobin / red blood cell counting blood segment 204 is first allocated to the hemoglobin / red blood cell counting segment, and then the white blood cell counting blood segment 203 is allocated to the white blood cell counting segment.

[0070] Afterwards, in step S150 , a third preset ratio of the blood sample near the end of the sampling needle is distributed to the white blood cell classification and counting section.

[0071] The leukocyte classification and counting unit is used to classify the leukocytes in the blood sample, that is, to classify the leukocytes into five categories: monocytes, lymphocytes and granulocytes (including eosinophils, neutrophils and basophils), and count each type of leukocytes. Specifically, the leukocyte classification and counting can be achieved by using a laser scattering method, using a laser to irradiate the leukocytes flowing through the detection area, detecting the optical signals of the leukocytes, and obtaining the morphology and size information of the leukocytes. Afterwards, the leukocytes can be divided into multiple groups according to the morphology and size information of the leukocytes, such as 3 groups corresponding to lymphocytes, monocytes, and eosinophils and 1 group corresponding to neutrophils and basophils, a total of 4 groups, and the number of leukocytes contained in each group is counted. The white blood cell classification and counting section counts the white blood cell classification (DIFF) and white blood cell classification and counting parameters, and the white blood cell classification and counting parameters are usually not output as report parameters. Therefore, the white blood cell classification and counting section mainly outputs the white blood cell classification results. For a given blood sample, even if a dilution effect occurs, the proportion of each type of white blood cell in the blood sample remains fixed, and therefore the white blood cell classification and counting section does not have high quantitative requirements for the blood sample.

[0072] Based on this, the blood separation method 100 of the embodiment of the present invention sets the white blood cell classification and counting blood segment 205 allocated to the white blood cell classification and counting unit at the top of the sampling needle away from the needle position. Since the blood sample has a dilution effect in the sample needle and the diluent in the pipeline and the tube wall, the farther away from the needle tip, the greater the dilution effect. If the blood sample at the top is allocated to other counting units with higher quantitative requirements, it will affect the accuracy of the measurement; in comparison, allocating the blood sample at the top to the white blood cell classification and counting unit with lower quantitative requirements for the blood sample can minimize the impact of the dilution effect on the measurement result.

[0073] As mentioned above, after collecting a blood sample, the sampling needle needs to continuously distribute the blood sample in multiple counting sections. For general blood cell analyzers, the amount of blood sample required for each counting section is relatively small, about a few microliters to more than ten microliters. Therefore, when distributing the blood sample, the needle tip of the sampling needle is generally controlled to be below the liquid surface of the bottom liquid of the counting section, so as to ensure that the blood sample will not adhere to the outer wall of the sampling needle in large quantities, thereby affecting the quantitative distribution. However, the needle tip of the sampling needle entering the bottom liquid of the counting section will bring about the problem of cross contamination. For example, the bottom liquid may affect the purity of the remaining blood sample inside the sampling needle.

[0074] In order to solve the above problem, an isolated blood segment can be set between each adjacent segment of blood from the first preset ratio of blood samples to the fifth preset ratio of blood samples, and the blood separation method of the embodiment of the present invention also includes discarding the isolated blood segment. Figure 2A In the embodiment shown, the isolated blood segments are distributed between the reticulocyte counting blood segment 202 and the leukocyte counting blood segment 203, between the leukocyte counting blood segment 203 and the hemoglobin / red blood cell counting blood segment 204, and between the hemoglobin / red blood cell counting blood segment 204 and the leukocyte differential counting blood segment 205; Figure 2B In the illustrated embodiment, the isolated blood segments are distributed between the reticulocyte counting blood segment 202 and the hemoglobin / red blood cell counting blood segment 204, between the hemoglobin / red blood cell counting blood segment 204 and the white blood cell counting blood segment 203, and between the white blood cell counting blood segment 203 and the white blood cell differential counting blood segment 205.

[0075] That is to say, after the blood sample is dispensed to the previous counting part and before the blood sample is dispensed to the next counting part, the sample with the preset dose in the sampling needle is discarded. After the sample diluted or contaminated by the base liquid is discarded, it can be avoided that the sample affects the measurement result of the next measurement module, ensuring that there is no cross contamination of the blood samples used by the two adjacent measurement modules.

[0076] In addition, in the blood separation method provided in this embodiment, the sampling needle only collects the blood sample once and then distributes it multiple times, so the amount of blood sample collected needs to be sufficient for all counting parts, and other losses need to be included, such as isolated blood segments, sample adhesion to the inner wall of the sampling needle, etc. Therefore, a certain amount of blood sample collected by the sampling needle should be left, and after distributing the blood sample to all counting parts and discarding each isolated blood segment, the sampling needle also includes the remaining final blood sample 206. After the leukocyte classification counting blood segment 205 is distributed to the leukocyte classification counting part, the remaining final blood sample 206 in the sampling needle can be discarded.

[0077] Based on the above description, according to the blood separation method of the sampling needle in the embodiment of the present application, the blood sample close to the first segment of the blood sample is allocated to the reticulocyte counting part that needs to output accurate optical PLT parameters, so as to minimize the adhesion effect of this segment of blood sample in the sample needle and the pipeline; the blood sample close to the end of the sampling needle is allocated to the white blood cell classification counting part that does not have high quantitative requirements, so as to reduce the impact caused by the dilution effect of the blood sample; thereby, each blood segment in the sampling needle is allocated to a suitable counting part, thereby improving the measurement performance of the blood cell analyzer.

[0078] Reference Figure 3 The embodiment of the present invention further provides a blood cell analyzer 300, which includes a control device 310, a sampling needle 320 and a counting unit 330. The counting unit 330 is divided into a white blood cell counting unit for measuring white blood cell (WBC) count, a white blood cell classification counting unit for measuring white blood cell classification (DIFF) and counting, a hemoglobin / red blood cell counting unit for measuring hemoglobin (HGB) / red blood cell (RBC) count, and a reticulocyte counting unit for measuring reticulocyte (RET). The control device 310 is used to control the sampling needle 320 to distribute blood samples to multiple counting units. Specifically, the control device 310 controls the sampling needle 320 to perform the following operations: collect a blood sample, and the collected blood sample is distributed into multiple sections in the sampling needle 320 in sequence; discard the first section of the blood sample close to the needle head; allocate a first preset proportion of the blood sample close to the first section of the blood sample to the reticulocyte counting section; allocate a second preset proportion of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section; allocate a third preset proportion of the blood sample close to the end of the sampling needle to the white blood cell classification counting section.

[0079] In one embodiment, allocating a second preset ratio of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section specifically includes: allocating a fourth preset ratio of the blood sample near the needle tip of the sampling needle in the second preset ratio to the white blood cell counting section; and allocating a fifth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio to the hemoglobin / red blood cell counting section.

[0080] In another embodiment, allocating a second preset ratio of the blood sample located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section specifically includes: allocating a fourth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio to the white blood cell counting section; and allocating a fifth preset ratio of the blood sample near the needle tip of the sampling needle in the second preset ratio to the hemoglobin / red blood cell counting section.

[0081] Exemplarily, an isolation blood segment is provided between each adjacent segment of blood from the blood sample of the first preset ratio to the blood sample of the fifth preset ratio, and the control device is further used to control the sampling needle to discard the isolation blood segment.

[0082] Exemplarily, after dispensing the blood sample of the third preset ratio, the control device 310 is further configured to control the sampling needle 320 to discard the last blood sample near the end of the sampling needle.

[0083] As an example, the control device 310 includes at least a processing component, a RAM, a ROM, a communication interface, a memory, and an I / O interface. The processing component, the RAM, the ROM, the communication interface, the memory, and the I / O interface communicate through a bus. The processing component can be a CPU, a GPU, or other chips with computing capabilities. The memory is equipped with various computer programs such as an operating system and an application program for the processing component to execute, and the data required to execute the computer program. In addition, during the blood sample analysis process, if there is data that needs to be stored locally, it can be stored in the memory. The I / O interface is composed of serial interfaces such as USB, IEEE 1394 or RS-232C, parallel interfaces such as SCSI, IDE or IEEE 1284, and analog signal interfaces composed of D / A converters and A / D converters. An input device composed of a keyboard, a mouse, a touch screen, or other control buttons is connected to the I / O interface, and the user can use the input device to directly input data to the control device. In addition, an output unit with a display function, such as an LCD screen, a touch screen, an LED display, etc., can also be connected to the I / O interface. The control device can output the processed data to the output unit for display in the form of image display data, such as analysis data, instrument operation parameters, etc. The communication interface can be an interface of any currently known communication protocol. The communication interface communicates with the outside world through a network. The control device can transmit data between any device connected through the network through the communication interface using a certain communication protocol.

[0084] Exemplarily, the sampling needle 320 may be fixed on a transmission mechanism, and the control device 310 controls the transmission mechanism to drive the sampling needle 320 to move to a specified position.

[0085] Exemplarily, the counting unit 330 has a reaction pool and a reagent supply component, the reagent supply component is used to provide reagents, and the reaction pool is used to provide a reaction site, so that the blood sample distributed to the counting unit 330 by the sampling needle 320 and the reagent provided by the reagent supply component can react in the reaction pool to obtain the sample to be detected. The counting unit 330 can also have a pipeline and a driving component (such as a syringe, an air pump, etc.) so that the blood cells in the blood sample to be detected can flow through the flow chamber of the optical detection system one by one.

[0086] Exemplarily, the blood cell analyzer 300 may also include an optical detection system. The control device 310 is also configured to receive a scattered light intensity signal from the optical detection system and obtain a blood cell detection result based on the scattered light intensity signal. The optical detection system is used to irradiate the blood cells in the blood sample to be detected when they flow through the flow chamber and collect the optical information generated by the blood cells due to the light irradiation. Exemplarily, the optical detection system may include a laser, a front light processing component, a flow chamber, and a scattered light detector. Among them, the flow chamber is configured to allow the blood cells in the blood sample to flow in a predetermined direction; the laser is configured to emit a laser beam toward the flow chamber; the front light processing component is arranged between the laser and the flow chamber along the emission direction of the laser beam, and is used to focus the laser beam emitted by the laser on the flow chamber, so that the blood cells flowing in the flow chamber can generate scattered light. The front light processing component may include a focusing component and a pinhole aperture, etc. The scattered light detector is configured to detect the light intensity of the scattered light.

[0087] Based on the above description, the blood cell analyzer according to the embodiment of the present application allocates the blood sample close to the first section of the blood sample to the reticulocyte counting part that needs to output accurate optical parameters, so as to minimize the adhesion effect of this section of the blood sample in the sample needle and the pipeline; allocates the blood sample close to the end of the sampling needle to the white blood cell classification counting part that does not have high quantitative requirements, so as to reduce the impact caused by the dilution effect of the blood sample; thereby, each blood section in the sampling needle is allocated to a suitable counting part, thereby improving the measurement performance of the blood cell analyzer.

[0088] Although example embodiments have been described herein with reference to the accompanying drawings, it should be understood that the above example embodiments are merely exemplary and are not intended to limit the scope of the present application to this. Those of ordinary skill in the art may make various changes and modifications therein without departing from the scope and spirit of the present application. All these changes and modifications are intended to be included within the scope of the present application as required by the appended claims.

[0089] Those of ordinary skill in the art will appreciate that the units and algorithm steps of each example described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, or a combination of computer software and electronic hardware. Whether these functions are performed in hardware or software depends on the specific application and design constraints of the technical solution. Professional and technical personnel can use different methods to implement the described functions for each specific application, but such implementation should not be considered to be beyond the scope of this application.

[0090] In the several embodiments provided in this application, it should be understood that the disclosed devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic, for example, the division of the units is only a logical function division, and there may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another device, or some features can be ignored or not executed.

[0091] In the description provided herein, a large number of specific details are described. However, it is understood that the embodiments of the present application can be practiced without these specific details. In some instances, well-known methods, structures and techniques are not shown in detail so as not to obscure the understanding of this description.

[0092] Similarly, it should be understood that in order to streamline the present application and help understand one or more of the various inventive aspects, in the description of the exemplary embodiments of the present application, the various features of the present application are sometimes grouped together into a single embodiment, figure, or description thereof. However, the method of the present application should not be interpreted as reflecting the following intention: the claimed application requires more features than the features clearly stated in each claim. More specifically, as reflected in the corresponding claims, the inventive point is that the corresponding technical problem can be solved with features less than all the features of a single disclosed embodiment. Therefore, the claims following the specific embodiment are hereby explicitly incorporated into the specific embodiment, wherein each claim itself serves as a separate embodiment of the present application.

[0093] It will be understood by those skilled in the art that, except for mutually exclusive features, all features disclosed in this specification (including the accompanying claims, abstracts and drawings) and all processes or units of any method or device disclosed in this specification may be combined in any combination. Unless otherwise expressly stated, each feature disclosed in this specification (including the accompanying claims, abstracts and drawings) may be replaced by an alternative feature that provides the same, equivalent or similar purpose.

[0094] In addition, those skilled in the art will appreciate that, although some embodiments described herein include certain features included in other embodiments but not other features, the combination of features of different embodiments is meant to be within the scope of the present application and form different embodiments. For example, in the claims, any one of the claimed embodiments can be used in any combination.

[0095] The various component embodiments of the present application can be implemented in hardware, or in software modules running on one or more processors, or in a combination thereof. It should be understood by those skilled in the art that a microprocessor or digital signal processor (DSP) can be used in practice to implement some or all of the functions of some modules in the article analysis device according to the embodiment of the present application. The present application can also be implemented as a device program (e.g., computer program and computer program product) for executing part or all of the methods described herein. Such a program implementing the present application can be stored on a computer-readable medium, or can have the form of one or more signals. Such a signal can be downloaded from an Internet website, or provided on a carrier signal, or provided in any other form.

[0096] It should be noted that the above embodiments illustrate the present application rather than limit the present application, and that those skilled in the art may design alternative embodiments without departing from the scope of the appended claims. In the claims, any reference symbol between brackets should not be constructed as a limitation to the claims. The word "comprising" does not exclude the presence of elements or steps not listed in the claims. The word "one" or "an" preceding an element does not exclude the presence of multiple such elements. The present application may be implemented by means of hardware including several different elements and by means of a suitably programmed computer. In a unit claim that lists several devices, several of these devices may be embodied by the same hardware item. The use of the words first, second, and third, etc. does not indicate any order. These words may be interpreted as names.

[0097] The above is only a specific implementation or description of a specific implementation of the present application, and the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. The protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A blood separation method using a sampling needle, characterized in that: The method comprises: Collecting a blood sample through a sampling needle, wherein the blood sample is sequentially distributed into multiple sections in the sampling needle; Discarding the first section of the blood sample near the needle tip in the sampling needle; distributing a first preset proportion of the blood sample in the sampling needle close to the first blood sample to a reticulocyte counting unit, wherein the reticulocyte counting unit is also used to output an optical platelet parameter; Distribute the blood sample of a second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section, wherein the blood sample of the second preset ratio is located between the blood sample of the first preset ratio and the blood sample of the third preset ratio; The blood sample of the third preset ratio near the end of the sampling needle is distributed to the leukocyte classification and counting section.

2. The blood separation method according to claim 1, characterized in that: The method of distributing the blood sample of the second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises: distributing a fourth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample to the white blood cell counting unit; A fifth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio of the blood sample is distributed to the hemoglobin / red blood cell counting part.

3. The blood separation method according to claim 1, characterized in that: The method of distributing the blood sample of the second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises: distributing a fourth preset proportion of the blood sample near the end of the sampling needle in the second preset proportion of the blood sample to the white blood cell counting section; A fifth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample is distributed to the hemoglobin / red blood cell counting section.

4. The blood separation method according to any one of claims 1 to 3, characterized in that: An isolated blood segment is provided between each adjacent segment of blood from the blood sample of the first preset ratio to the blood sample of the fifth preset ratio, and the method further includes: discarding the isolated blood segment.

5. The blood separation method according to claim 1, characterized in that: After distributing the blood sample of the third preset ratio, the method further includes: The final blood sample near the end of the sampling needle is discarded.

6. A blood cell analyzer, characterized in that: The blood cell analyzer comprises: a sampling needle, a control device and a counting unit, wherein the control device is used to control the sampling needle to distribute blood samples to multiple counting units: The sampling needle collects a blood sample, and the blood sample is sequentially distributed into multiple sections in the sampling needle; Discarding the first section of the blood sample near the needle tip in the sampling needle; distributing a first preset proportion of the blood sample in the sampling needle close to the first blood sample to a reticulocyte counting unit, wherein the reticulocyte counting unit is also used to output an optical platelet parameter; Distribute the blood sample of a second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section, wherein the blood sample of the second preset ratio is located between the blood sample of the first preset ratio and the blood sample of the third preset ratio; A third preset ratio of the blood sample near the end of the sampling needle is distributed to the leukocyte differential counting section.

7. The blood cell analyzer according to claim 6, characterized in that: The method of distributing the blood sample of the second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises: distributing a fourth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample to the white blood cell counting unit; A fifth preset ratio of the blood sample near the end of the sampling needle in the second preset ratio of the blood sample is distributed to the hemoglobin / red blood cell counting part.

8. The blood cell analyzer according to claim 6, characterized in that: The method of distributing the blood sample of the second preset ratio located in the middle section of the sampling needle to the white blood cell counting section and the hemoglobin / red blood cell counting section comprises: distributing a fourth preset proportion of the blood sample near the end of the sampling needle in the second preset proportion of the blood sample to the white blood cell counting section; A fifth preset proportion of the blood sample close to the sampling needle tip among the second preset proportion of the blood sample is distributed to the hemoglobin / red blood cell counting section.

9. The blood cell analyzer according to any one of claims 6 to 8, characterized in that: An isolation blood segment is provided between each adjacent segment of blood from the blood sample of the first preset ratio to the blood sample of the fifth preset ratio, and the control device is further used to control the sampling needle to discard the isolation blood segment.

10. The blood cell analyzer according to claim 6, characterized in that: After dispensing the blood sample of the third preset ratio, the control device is further used for: The sampling needle is controlled to discard the terminal blood sample near the end of the sampling needle.

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