Microsphere-based electrospray device

Abstract

The present application relates to the technical field of microsphere preparation, and particularly relates to a microsphere-based electrospray device, which comprises a nozzle, a conductive piece and a power supply, the nozzle has a solution channel, the solution channel has an inlet and an outlet, the conductive piece is arranged at a position adjacent to the outlet, the power supply comprises a first output end and a second output end, the first output end is connected with the outlet to provide a first electric field, the second output end is connected with the conductive piece to provide a second electric field, and the direction of the first electric field is arranged orthogonally to the direction of the second electric field, the microsphere-based electrospray device can improve the uniformity of the prepared microspheres.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microsphere preparation, and particularly relates to a microsphere-based electrospray device. BACKGROUND

[0002] In the related art, oil phase solution is mostly used for preparing microspheres. However, the use of oil phase solution and the drying method are harsh, which can destroy the biological activity and cannot realize the encapsulation of cells. Therefore, the use of aqueous solution can maintain good biological activity. However, compared with oil phase solution, the surface tension of aqueous solution is large, and the radial restraint force is weak, so that the Taylor cone formed at the nozzle during the preparation of microspheres by the traditional electrospray device is fragile and easy to lose stability, resulting in that the size of the prepared microspheres is not uniform. SUMMARY

[0003] The present application aims to at least solve one of the technical problems in the related art. To this end, the embodiments of the present application propose a microsphere-based electrospray device, which can improve the uniformity of the prepared microspheres.

[0004] The microsphere-based electrospray device according to the embodiments of the present application comprises: a nozzle, the nozzle has a solution channel therein, the solution channel has an inlet and an outlet; a conductive piece, the conductive piece is arranged in a spaced manner with the nozzle, and the conductive piece is located on a side adjacent to the outlet; and a power supply, the power supply comprises a first output end and a second output end, the first output end is connected with the outlet to provide a first electric field, the second output end is connected with the conductive piece to provide a second electric field, and the direction of the first electric field is arranged in an orthogonal manner with the direction of the second electric field.

[0005] The microsphere-based electrospray device according to the embodiments of the present application can improve the uniformity of the prepared microspheres.

[0006] In some embodiments, the conductive piece is provided with a first through hole, the first through hole extends along the direction of the conductive piece towards the nozzle, and the outlet is located on the central extension line of the first through hole.

[0007] In some embodiments, on the cross section of the conductive piece, the cross-sectional size of the first through hole gradually increases in the direction towards the outlet.

[0008] In some embodiments, the microsphere-based electrospray device further comprises an insulating piece, the insulating piece has a mounting hole therein, the conductive piece is detachably connected with the insulating piece, and the conductive piece is located in the mounting hole.

[0009] In some embodiments, the microsphere-based electrospray device further comprises a collection plate, the collection plate is located on a side of the conductive piece away from the outlet.

[0010] In some embodiments, the microsphere-based electrospray device further comprises a support, the support comprising a support body, a first support and a second support, the first support and the second support being arranged at intervals in the length direction of the support body, the first support being connected with the nozzle, and the second support being connected with the insulating member.

[0011] In some embodiments, the first support is provided with a second through hole, one end of the second through hole being adapted to communicate with a solution source, and the other end of the through hole being in communication with the inlet.

[0012] In some embodiments, the second support comprises a support portion and a connecting portion connected in sequence, the support portion being connected with the insulating member, and the connecting portion being detachably connected with the support body.

[0013] In some embodiments, the first support and the second support are capable of approaching or moving away from each other in the length direction of the support body.

[0014] In some embodiments, in the cross section of the conductive member, the included angle between the inner wall surface of the first through hole and the axis of the first through hole is A, and 0°≤A<90°. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 is a schematic view of a microsphere-based electrospray device according to an embodiment of the present application.

[0016] Figure 2 is a sectional view of a microsphere-based electrospray device according to an embodiment of the present application.

[0017] Figure 3 is a side view of a microsphere-based electrospray device according to an embodiment of the present application.

[0018] Figure 4 is a potential distribution diagram of a single electric field according to an embodiment of the present application.

[0019] Figure 5 is a potential distribution diagram of a composite electric field of a first electric field and a second electric field according to an embodiment of the present application.

[0020] Figure 6 is a distribution diagram of the size of microspheres prepared under a single electric field and the size of microspheres prepared under a composite electric field according to an embodiment of the present application.

[0021] Figure 7 is a schematic view of the roundness of microspheres prepared under a single electric field and the roundness of microspheres prepared under a composite electric field according to an embodiment of the present application.

[0022] Figure 8 is a quantitative distribution diagram of microspheres prepared under a single electric field according to an embodiment of the present application.

[0023] Figure 9 is a microsphere light microscope image prepared under a single electric field of an embodiment of the present application.

[0024] Figure 10 is a microsphere profile image prepared under a single electric field of an embodiment of the present application.

[0025] Figure 11 is a microsphere quantitative distribution image prepared under a composite electric field of an embodiment of the present application.

[0026] Figure 12 is a microsphere light microscope image prepared under a composite electric field of an embodiment of the present application.

[0027] Figure 13 is a microsphere profile image prepared under a single electric field of an embodiment of the present application.

[0028] Figure 14 is a live image of a cell death and live staining of cells in a cell-containing hydrogel microsphere prepared under a composite electric field of an embodiment of the present application.

[0029] Figure 15 is a death image of a cell death and live staining of cells in a cell-containing hydrogel microsphere prepared under a composite electric field of an embodiment of the present application.

[0030] Reference Signs:

[0031] Nozzle 1, solution passage 11, inlet 111, outlet 112,

[0032] Conductive member 2, first through-hole 21,

[0033] Insulating member 3, mounting hole 31,

[0034] Collecting plate 4,

[0035] Support member 5, support body 51, first support member 52, second through-hole 521, second support member 53, support portion 531, connection portion 532. DETAILED DESCRIPTION

[0036] Embodiments of the present application will be described in detail below, examples of which are shown in the accompanying drawings. The embodiments described below by reference to the drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.

[0037] Reference Figures 1 to 3 As shown in the drawing, the microsphere-based electrospray device of an embodiment of the present application includes a nozzle 1, a conductive member 2, and a power source (not shown).

[0038] The nozzle 1 has a solution channel 11 with an inlet 111 and an outlet 112. The electrically conductive member 2 is arranged spaced apart from the nozzle 1 and is located on a side adjacent to the outlet 112. The power supply includes a first output (not shown) connected to the outlet 112 to provide a first electric field and a second output (not shown) connected to the electrically conductive member 2 to provide a second electric field, the directions of the first electric field and the second electric field are arranged orthogonally.

[0039] Specifically, referring to Figure 2 As shown, the solution channel 11 extends in the nozzle 1 in the up-down direction, and the inlet 111 is located above and the outlet 112 is located below. The electrically conductive member 2 is located below the nozzle 1, and the electrically conductive member 2 is arranged adjacent to the outlet 112.

[0040] Optionally, the first output connected to the outlet 112 provides an axial electric field, and it should be noted that the direction of the axial electric field is the up-down direction, and the second output connected to the electrically conductive member 2 provides a radial electric field, and it should be noted that the direction of the radial electric field is the left-right direction. The axial action of the axial electric field on the droplet causes the front end of the droplet at the outlet 112 to form a cone, and the radial electric field makes the cone formed by the front end of the droplet more stable. Compared with the process of preparing microspheres by the water phase solution in the related art electric spray device, only the conical shape formed under the action of a single direction electric field, the present embodiment increases the radial electric field to increase the radial restraint force on the droplet, and quantitatively supplements the radial electric field, so that the cone formed by the droplet is more stable and has higher rigidity, and the uniformity of the prepared microspheres is improved.

[0041] Referring to Figures 4 to 5 As shown, the present embodiment increases the electrically conductive member 2 to make the droplet at the outlet 112 receive a greater radial restraint force. Compared with a single electric field, the electric spray device with a radial electric field can provide a greater radial restraint force to the droplet at the outlet 112 when preparing microspheres, overcoming the instability of the cone formed by the water phase solution under the action of only the axial electric field. The radial electric field makes the droplet form a more stable cone, and improves the stability of the droplet forming a cone under the same flux.

[0042] For example, the distance between the electrically conductive member 2 and the outlet 112 in the up-down direction can be set to 4mm, so that the second electric field acts on the cone formation process of the droplet, and the stability of the droplet forming a cone is improved.

[0043] For example, since the force of the axial electric field is much greater than the force of gravity during the preparation of the microspheres, i.e., the force of gravity of the droplets can be ignored during the preparation of the microspheres, when the solution channel 11 extends in the nozzle 1 in the up-down direction, the positions of the inlet 111 and the outlet 112 can be arranged reversely, i.e., the inlet 111 is located below and the outlet 112 is located above. When the solution channel 11 extends in the nozzle 1 in the left-right direction, the inlet 111 is located on the left and the outlet 112 is located on the right, or the inlet 111 is located on the right and the outlet 112 is located on the left, as long as the droplet at the outlet 112 can form a cone under the action of the first electric field and the second electric field, the different directions of the inlet 111 and the outlet 112 of the solution channel 11 make the direction of the outlet 112 of the nozzle 1 diversified, and the electric spraying device is diversified.

[0044] For example, the positions of the first output end and the second output end are reversible, i.e., the first output end is connected with the conductive member 2 to provide a radial electric field, and the second output end is connected with the outlet 112 to provide an axial electric field. The first output end and the second output end are independent of each other, i.e., the voltage output by the first output end and the voltage output by the second output end are independent of each other. The output voltage of the first output end can be the same as or different from the output voltage of the second output end, i.e., the output voltage of the first output end can be greater than or less than the output voltage of the second output end. The first output end is a positive electrode, connected with the outlet 112 through a wire, and the second output end is also a positive electrode, connected with the conductive member 2 through a wire.

[0045] In some embodiments, the conductive member 2 is provided with a first through hole 21 extending along the conductive member 2 towards the nozzle 1, and the outlet 112 is located on the center extension line of the first through hole 21.

[0046] Specifically, the first through hole 21 penetrates the conductive member 2 in the up-down direction, the first through hole 21 is located below the outlet 112, and the extension line of the outlet 112 in the up-down direction passes through the center point of the first through hole 21.

[0047] Optionally, the conductive member 2 is metal, and any conductive body that can conduct electricity can be the conductive member 2.

[0048] For example, the conductive member 2 can be an aluminum foil, and can also be a carbon-coated aluminum foil. Since the aluminum foil has good electrical conductivity, the loss in the process of conducting electricity of the conductive member 2 can be reduced.

[0049] For example, the number of the conductive member 2 can be one, and the shape of the conductive member 2 can be a circular ring or a square. The number of the conductive member 2 can also be multiple, and the multiple conductive members 2 are arranged uniformly in the circumferential direction of the first through hole 21. For example, the number of the conductive member 2 is four, and the four conductive members 2 are spaced 90° from each other in the circumferential direction of the first through hole 21. By different setting modes of the conductive member 2, the stability of the electric field generated by the conductive member 2 can be adjusted, the radial force of the conductive member 2 on the liquid droplet is improved, the cone shape formed by the liquid droplet is more stable, and the uniformity of the prepared microspheres is further improved.

[0050] Alternatively, the multiple conductive members 2 are connected in sequence in the up-down direction.

[0051] In some embodiments, in the cross section of the conductive member 2, the cross-sectional size of the first through hole 21 gradually increases in the direction towards the outlet 112.

[0052] Optionally, in the cross section of the conductive member 2 in the left-right direction, the cross-sectional size of the first through hole 21 gradually increases in the upward direction, so that in the up-down direction of the conductive member 2, the electric field force generated by the upper part of the conductive member 2 is smaller than that generated by the lower part of the conductive member 2. When the conductive member 2 acts on the liquid droplet, the force generated by the upper part of the conductive member 2, i.e. the part close to the outlet 112, on the liquid droplet is smaller than that generated by the lower part of the conductive member 2, i.e. the part away from the outlet 112, so that the lower part of the cone shape formed by the liquid droplet receives a greater radial force, the stability of the cone shape formed by the liquid droplet is improved, and the uniformity of the prepared microspheres is further improved.

[0053] For example, when the number of the conductive member 2 is multiple, and the multiple conductive members 2 are connected in sequence in the up-down direction, the inner wall surfaces of the adjacent two conductive members 2 are in the same plane, i.e. the taper of the inner wall surfaces of the adjacent two conductive members 2 is the same.

[0054] In some embodiments, the electrospray device based on microspheres further comprises an insulating member 3, the insulating member 3 has a mounting hole 31 therein, the conductive member 2 is detachably connected with the insulating member 3, and the conductive member 2 is located in the mounting hole 31.

[0055] Specifically, the mounting hole 31 penetrates the insulating member 3 in the up-down direction, and is used for mounting the conductive member 2.

[0056] Optionally, the outer contour of the conductive member 2 is connected with the inner contour of the insulating member 3, the insulating member 3 fixes the conductive member 2, and the conductive member 2 is located directly below the outlet 112.

[0057] For example, the conductive part 2 and the insulating part 3 can be connected by means of adhesion, which can make the connection between the conductive part 2 and the insulating part 3 more compact, make the force of the conductive part 2 on the liquid droplet more balanced, improve the stability of the liquid droplet in the form of a cone under the action of the conductive part 2, and further improve the uniformity of the microspheres prepared.

[0058] In some embodiments, the microsphere-based electrospray device further comprises a collection plate 4, which is located on the side of the conductive part 2 away from the outlet 112.

[0059] Specifically, the collection plate 4 is located below the conductive part 2.

[0060] Optionally, the collection plate 4 is grounded, providing a zero potential point, and there is a potential difference between the first electric field and the collection plate 4, so that the liquid droplet is subjected to an axial force from top to bottom. By adjusting the distance between the nozzle 1 and the collection plate 4, the liquid droplet at the outlet 112 is subjected to an axial force, which facilitates the adjustment of the cone formed by the liquid droplet under the action of the first electric field. Moreover, by adjusting the distance between the nozzle 1 and the collection plate 4, the collection position of the liquid droplet formed after the liquid droplet forms a cone and breaks into a jet can be adjusted, and the collection efficiency of the collection plate 4 can be improved.

[0061] Optionally, the size of the collection plate 4 in the left-right direction is greater than the size of the first through hole 21 in the left-right direction, so that the collection plate 4 can collect the liquid droplet passing through the first through hole 21.

[0062] For example, the collection plate 4 can be placed on the collection solution, and the collection solution can be prepared according to the different solutions of the microspheres to be prepared, and different collection solutions can be set correspondingly to improve the collection efficiency of the collection plate 4.

[0063] For example, the collection plate 4 is movable relative to the conductive part 2, for example, the collection plate 4 can move in the front-back or left-right direction to adjust the collection position of the microspheres, or the collection plate 4 can move in the up-down direction to adjust the interval distance between the collection plate 4 and the conductive part 2 in the up-down direction, so as to improve the uniformity of the microspheres.

[0064] In some embodiments, the microsphere-based electrospray device further comprises a support 5, which comprises a support body 51, a first support 52 and a second support 53, the first support 52 and the second support 53 are arranged at intervals in the length direction of the support body 51 (such as the up-down direction shown). Figure 2 The first support 52 is connected with the nozzle 1, and the second support 53 is connected with the insulating part 3.

[0065] Specifically, the support body 51 is connected with the first support 52 and the second support 53 respectively, for supporting the first support 52 and the second support 53, and the first support 52 is located above the second support 53, the first support 52 is arranged above the nozzle 1 and connected with the nozzle 1, for fixing the nozzle 1, and the second support 53 is arranged below the conductive part 2 and connected with the insulating part 3, for supporting and fixing the insulating plate.

[0066] For example, the support body 51 is the insulating part 3 as the first support 52 and the second support 53, avoiding interference with the first electric field and the second electric field, and improving the efficiency of the first electric field and the second electric field on the droplet forming cone.

[0067] For example, the first support 52 is integrally formed with the support body 51, improving the structural stability between the first support 52 and the support body 51.

[0068] For example, the first support 52 is connected with the nozzle 1 through bolts, forming a detachable connection between the first support 52 and the nozzle 1, facilitating subsequent replacement of the nozzle 1, and the first support 52 is integrally formed with the support body 51, so that the first support 52 has a better fixing effect on the nozzle 1, ensuring the stability of the droplets flowing out of the outlet 112.

[0069] Optionally, by adjusting the distance between the first support 52 and the second support 53 in the up-down direction, the distance between the outlet 112 of the nozzle 1 and the conductive part 2 can be adjusted, so as to adjust the different radial forces of the conductive part 2 on the droplets at the outlet 112, adjust the position of the droplets subjected to the radial force, and improve the stability of the cone formed by the droplets.

[0070] In some embodiments, the first support 52 is provided with a second through hole 521, one end of the second through hole 521 is adapted to communicate with the solution source, and the other end of the through hole communicates with the inlet 111.

[0071] Specifically, the second through hole 521 penetrates the first support 52 in the up-down direction, the upper end of the second through hole 521 communicates with the solution source, for transmitting the solution into the second through hole 521, the lower end of the second through hole 521 communicates with the inlet 111, for transmitting the solution in the second through hole 521 into the solution channel 11, and the arrangement of the second through hole 521 makes the solution source outside communicate with the solution channel 11, improving the transmission efficiency of the solution.

[0072] In some embodiments, the second support 53 includes a support part 531 and a connecting part 532 connected in sequence, the support part 531 is connected with the insulating part 3, and the connecting part 532 is detachably connected with the support body 51.

[0073] Specifically, the second support 53 is sequentially provided with a support portion 531 and a connecting portion 532 from left to right, the support portion 531 is connected with the insulating piece 3 and is used for supporting and fixing the insulating piece 3, the left end of the connecting portion 532 is connected with the support portion 531, the right end of the connecting portion 532 is connected with the support body 51, and the second support 53 is detachably connected with the support body 51 through the detachable connection between the right end of the connecting portion 532 and the support body 51.

[0074] In some embodiments, the first support 52 and the second support 53 are close to or away from each other in the length direction of the support body 51.

[0075] Specifically, the second support 53 is located below the first support 52, and the second support 53 is detachably connected with the support body 51, so that the position of the second support 53 in the up-down direction relative to the first support 52 can be adjusted, that is, the second support 53 can be arranged close to the first support 52, and the second support 53 can also be arranged away from the first support 52, by adjusting the distance between the second support 53 and the first support 52 in the up-down direction, the distance between the conductive piece 2 and the outlet 112 is adjusted, and then the position of the liquid droplet at the outlet 112 subjected to the radial force is adjusted, and the stability of the liquid droplet forming a conical surface is improved.

[0076] In some embodiments, the included angle between the inner wall surface of the first through hole 21 and the axis of the first through hole 21 on the cross section of the conductive piece 2 is A, and 0°≤A<90°.

[0077] Specifically, the included angle between the inner wall surface of the first through hole 21 and the axis of the first through hole 21 on the cross section of the conductive piece 2, that is, on the cross section of the conductive piece 2 in the left-right direction, is A, by adjusting the angle of the included angle A, the different forces of the liquid droplet above and below the conductive piece 2 can be adjusted, and then the radial force of the liquid droplet forming a cone is adjusted, the stability of the liquid droplet forming a conical surface is improved, and then the uniformity of the prepared microspheres is improved.

[0078] For example, A can be 0°, 5°, 10°, 30°, 49.3°, 49.7°, 60°, 80°, 89°, and the included angle is selected as 49.3° in the embodiment, the angle of the included angle is set to be the same as the angle of the Taylor cone, the conductive piece 2 is in the shape of the similar enlargement of the Taylor cone, the radial constraint electric field generated by the conductive piece 2 is more conducive to the conical formation of the liquid droplet, and after the radial electric field is increased, the conical stability of the liquid droplet at the outlet 112 is stronger in the process of preparing the microspheres, and when affected by the outside world, the liquid droplet can quickly return to a stable state, the preparation process of a larger flow is realized, and then the preparation efficiency of the microspheres is improved.

[0079] Referring to Figure 6 and Figure 7As shown, the size distribution and roundness of the microspheres prepared by the composite electric field composed of the first electric field and the second electric field are shown, the particle size of the microspheres prepared by the composite electric field is 90.3, the variance is 4.545, and the roundness is 0.93, while the particle size of the microspheres prepared by the single electric field is 112.8, the variance is 42.74, and the roundness is 0.91. Through the radial constraint of the droplets by the composite electric field, the effect of the droplets under the composite electric field to form a cone is more stable, and the process of the jet to break to form microspheres is also more stable, and the difference between each microsphere prepared is smaller. Compared with the microspheres prepared by the single electric field, the microspheres prepared by the composite electric field have higher uniformity, narrower particle size distribution, better roundness, fewer outliers, and higher quality of the microspheres.

[0080] Referring to Figures 8 to 13 As shown, the outline of the light microscope image of the prepared microspheres under the microscope is subjected to image binarization processing to obtain a profile of the microspheres, and the profile is subjected to solid filling hole processing to obtain a morphology of the microspheres. Compared with the microspheres prepared by the single electric field, the microspheres prepared by the electric spraying device under the composite electric field composed of the first electric field and the second electric field have higher uniformity.

[0081] Referring to Figures 14-15 As shown, the electric spraying device using the composite electric field composed of the first electric field and the second electric field is used for HepG2 hydrogel cell encapsulation, and cell culture is performed for 7 days. After 7 days of culture, the microspheres have good biocompatibility. The living cells prepared under the composite electric field (see Figure 14 and Figure 15 As shown) have a large number of signal expressions, and the dead cells are less (see Figure 15 As shown), the cells can exchange nutrients and exclude waste normally, and survive in the microspheres, which indicates that the microspheres prepared by the embodiment of the present application have good biocompatibility.

[0082] In the description of the present application, it should be understood that the orientations or positional relationships indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0083] In addition, the terms "first", "second", etc. are used only to describe different instances, and are not used to indicate or imply relative importance or a number of indications of the technical features indicated. Therefore, the features defined as "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise explicitly specified and limited.

[0084] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected or in communication with each other; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0085] In the present application, unless otherwise explicitly specified and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0086] In the present application, the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" mean that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine different embodiments or features of different embodiments or examples described in the present specification without contradiction.

[0087] Although the embodiments of the present application have been shown and described above, it is understood that the above embodiments are exemplary and cannot be construed as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application.

Claims

1. A microsphere-based electrospray device, characterized by, The application relates to a nozzle device, comprising: a nozzle having a solution channel with an inlet and an outlet; an electrically conductive member arranged in proximity to the nozzle and on a side adjacent to the outlet; a first through hole is arranged on the electrically conductive member and extends along the electrically conductive member towards the nozzle, and the outlet is located on a central extension line of the first through hole; in a cross section of the electrically conductive member, a cross section size of the first through hole gradually increases in a direction towards the outlet, an included angle between an inner wall surface of the first through hole and an axis of the first through hole is A, and 0<=A<90; a power supply comprising a first output end and a second output end, the first output end is connected with the outlet to provide a first electric field, the second output end is connected with the electrically conductive member to provide a second electric field, and the first electric field is arranged in a direction perpendicular to a direction of the second electric field.

2. The microsphere-based electrospray device of claim 1, wherein, an insulating member having a mounting hole, the electrically conductive member is detachably connected with the insulating member, and the electrically conductive member is located in the mounting hole.

3. The microsphere-based electrospray device of claim 2, wherein, a collecting plate located on a side of the electrically conductive member away from the outlet.

4. The microsphere-based electrospray device of claim 3, wherein, a support member comprising a support body, a first support member and a second support member, the first support member and the second support member are arranged in proximity to each other in a length direction of the support body, the first support member is connected with the nozzle, and the second support member is connected with the insulating member.

5. The microsphere-based electrospray device of claim 4, wherein, a second through hole is arranged on the first support member, one end of the second through hole is adapted to communicate with a solution source, and the other end of the second through hole communicates with the inlet.

6. The microsphere-based electrospray device of claim 4, wherein, the second support member comprises a support part and a connecting part connected in sequence, the support part is connected with the insulating member, and the connecting part is detachably connected with the support body.

7. The microsphere-based electrospray device of claim 6, wherein, the first support member and the second support member can be close to or away from each other in the length direction of the support body.

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