A method for extracting active peptides from sea cucumber flowers and its application

Abstract

The present invention relates to the field of sea cucumber processing technology. In order to solve the problem that the existing sea cucumber flower processing and extraction method causes a large amount of loss of sea cucumber flower nutrients and low yield, a method for extracting sea cucumber flower active peptides and applications are disclosed. The method for extracting sea cucumber flower active peptides comprises the following steps: cleaning, crushing, enzymolysis, filtering, concentrating, drying and bagging. In the extraction method of the present invention, the enzymolysis process is pure green and without addition. Liquor is added during enzymolysis to increase the physical denaturation rate of protein, reduce the loss of nutrients, and increase the income of effective peptide powder; the sea cucumber flower oligopeptide prepared by the present invention contains a variety of nutrients, including sea cucumber polypeptides, sea cucumber mucopolysaccharides, sea cucumber saponins, chondroitin sulfate, alum and calcium iron zinc selenium, and some components are 3-4 times the content of sea cucumber; the whole process reaction conditions are mild, no organic solvent is used, the nutrient components are less damaged, and the content of sea cucumber sulfate proteoglycan polymer (chondroitin sulfate) is as high as more than 50%.

Description

Technical Field

[0001] The invention relates to the technical field of sea cucumber processing, and in particular to a method for extracting active peptides from sea cucumber flowers and applications thereof. Background Art

[0002] Sea cucumber flowers are produced during the processing of sea cucumbers. They contain high levels of protein, sea cucumber mucopolysaccharides, sea cucumber saponins, etc. Some active substances are 3-4 times higher than those of sea cucumbers. They have high anti-tumor, anti-radiation, immunity-enhancing, anti-aging effects, etc., especially peptide nutrients with a molecular size of 200 Daltons-1000 Daltons or less, which are more easily absorbed by the human body. At present, few sea cucumber flowers are processed, and most of them are discarded, or acid is used for soaking before and during enzymatic hydrolysis, so that the nutrients of sea cucumber flowers undergo chemical reactions and denaturation, and then neutralized with alkali. The production process is highly polluting, pungent, and has great nutritional damage. There is little or no sea cucumber sulfate proteoglycan (chondroitin sulfate), the ingredients are single, and the product tastes average. Summary of the invention

[0003] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a method for extracting active peptides from sea cucumber flowers and their application.

[0004] The invention relates to a method for extracting active peptides from sea cucumber flowers and application thereof. Active substances in sea cucumber flowers are hydrolyzed into small molecule peptides by active biological enzymes, so that human body absorption and utilization rate are improved. The method for extracting active peptides from sea cucumber flowers comprises the following steps: washing, soaking, stirring, adding biological enzymes for hydrolysis, adding solid-state fermented liquor to accelerate the hydrolysis, and then injecting the hydrolyzed liquid into a centrifuge for filtration, vacuum concentrating, drying, and packaging into bags.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A method for extracting active peptides from sea cucumber flowers comprises the following steps:

[0007] Step 1: Wash the sea cucumber flowers, soak them in water, and then use boiling water to cook the soaked sea cucumber flowers, then blend and homogenize them to obtain a homogenate.

[0008] Step 2: adding active biological protease and solid-state fermented liquor to the homogenate for enzymolysis to obtain an enzymolysis solution;

[0009] Step 3: heating the enzymatic hydrolysate to inactivate the active biological protease, and then coarse filtering to obtain a coarse filtrate;

[0010] Step 4: ultrafiltering the crude filtrate to obtain an ultrafiltrate, and concentrating the ultrafiltrate to obtain a clarified liquid;

[0011] Step 5: Dry the clarified liquid and pack it into bags to obtain sea cucumber flower oligopeptide.

[0012] Preferably, in step 1, the sea cucumber flowers are soaked in water for 2 hours.

[0013] Preferably, in step 2, the temperature of enzymolysis is 45° C. and the duration of enzymolysis is 4 hours.

[0014] Preferably, in step 2, 1% by weight of the homogenate of solid-state fermented liquor is added during enzymatic hydrolysis.

[0015] Preferably, in step 3, the temperature of heating the enzymatic hydrolysate is 90° C. and the heating time is 15 minutes.

[0016] Preferably, in step 4, the crude filtrate is ultrafiltered using a centrifuge.

[0017] Preferably, in step 5, after the clarified liquid is dried, a filler, a flavoring agent, a plasticizer, and a dissolving agent are added, and then the liquid is packaged.

[0018] Preferably, the filler, flavoring agent, plasticizer, and dissolving agent are one or more of fructooligosaccharide, xylitol, maltooligosaccharide, double red rose powder, grapefruit powder, sea buckthorn powder, blueberry powder, acerola cherry powder, and magnesium stearate.

[0019] The beneficial effects of the present invention are:

[0020] The enzymatic hydrolysis process in the extraction method of the present invention is purely green and does not require acid or alkali chemical addition. Solid-state fermented liquor is added during enzymatic hydrolysis to increase protein denaturation speed, reduce nutrient loss, increase effective peptide powder yield, and preserve nutrition.

[0021] Solid-state fermented liquor contains more than 20 ingredients, including acetaldehyde, acetal, propionaldehyde, butyraldehyde, n-butyraldehyde, isobutyraldehyde, isovaleraldehyde, hexanal, phenylacetaldehyde, isovaleraldehyde, acetone, diacetyl, and 3-hydroxybutanone. They are hydroxylated compounds and can form hydrogen bond structures with proteins, accelerate the loosening of sea cucumber and sea cucumber intestinal egg molecules, speed up enzymatic hydrolysis, and increase profits. However, hydrogen bonds are not chemical bonds, and there is no chemical bond breaking during the whole process of change. In this way, the loss of sea cucumber sulfate proteoglycans (chondroitin sulfate) is small and abundant, with high overall profits, and it is rich in trace elements such as calcium, iron, zinc, selenium, and alum, and sea cucumber mucopolysaccharides. The ingredients in the warm enzymatic liquor are oxidized with the trimethylamine in the raw materials to form trimethylamine oxide, so that the enzymatic solution has no fishy smell, and the dried sea cucumber flower oligopeptides are delicious and have a good taste, which is easy for people to accept and eat, and convenient for market development.

[0022] The active substances in the sea cucumber flower prepared by the present invention have various nutrients, including sea cucumber polypeptides, sea cucumber mucopolysaccharides, sea cucumber saponins, etc.; the reaction conditions of the entire process are mild, no organic solvents are used, and the nutrients are less damaged. The molecules of the dried sea cucumber flower oligopeptides are relatively small, 80% of which are less than 1000 Daltons. The sea cucumber flower oligopeptides contain sea cucumber sulfated proteoglycan polymers (chondroitin sulfate) accounting for more than 50%. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a process flow chart of a method for extracting active peptides from sea cucumber flowers proposed in an embodiment of the present invention. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0025] Example 1

[0026] 50 kg of chilled sea cucumber flowers were collected, soaked, crushed, matured, and homogenized, and 250 kg of water, 100 g of neutral protease, and 3 kg of solid-fermented liquor were added, and the mixture was stirred until fully dissolved. The enzymatic reaction was carried out at 45 ° C for 4 hours; after the enzymatic hydrolysis, the mixture was kept warm at 90 ° C for 15 minutes to inactivate the protease; the processed liquid was coarsely filtered, ultrafiltered and desalted with a sodium filter membrane, the ultrafiltration filtrate was vacuum evaporated, concentrated to 30% of the solid content, and spray-dried to obtain 0.75 kg of sea cucumber flower oligopeptide, which contained 51.6% of sea cucumber sulfated proteoglycan polymer (chondroitin sulfate).

[0027] Example 2

[0028] 50 kg of chilled sea cucumber flowers were collected, soaked, crushed, matured, and homogenized, and 250 kg of water and 100 g of neutral protease were added. The flowers were stirred until fully dissolved, and the enzymatic reaction was carried out at 45 ° C for 4 hours; after the enzymatic hydrolysis, the flowers were kept warm at 90 ° C for 15 minutes to inactivate the protease; the processed liquid was coarsely filtered, ultrafiltered and desalted with a sodium filter membrane, the ultrafiltration filtrate was vacuum evaporated, concentrated to 30% of the solid content, and spray-dried to obtain sea cucumber intestinal egg oligopeptide; 0.4 kg of dry material was obtained, and the proportion of sea cucumber sulfated proteoglycan polymer (chondroitin sulfate) was measured to be 20%.

[0029] Example 3

[0030] 50 kg of chilled sea cucumber flowers were collected, 0.5 kg of citric acid was added during soaking, then crushed, matured, homogenized, 250 kg of water and 100 g of neutral protease were added, stirred until fully dissolved, and enzymolysis reaction was carried out at 45 ° C for 4 hours; after enzymolysis, it was kept warm at 90 ° C for 15 minutes to inactivate the protease; the processing liquid was coarsely filtered, ultrafiltered and desalted with a sodium filter membrane, the ultrafiltration filtrate was evaporated in a vacuum, concentrated to 30% of the solid content, and spray-dried to obtain sea cucumber flower oligopeptides; 0.5 kg of dry matter was obtained, and the proportion of sea cucumber sulfate proteoglycan polymer (chondroitin sulfate) was measured to be 10%.

[0031] Example 4

[0032] 50 kg of chilled sea cucumber flowers were collected, ethanol was added during soaking, then they were crushed, matured, and homogenized, and 250 kg of water and 100 grams of neutral protease were added. The mixture was stirred until fully dissolved and subjected to enzymatic hydrolysis at 45 ° C for 4 hours. After enzymatic hydrolysis, the mixture was kept warm at 90 ° C for 15 minutes to inactivate the protease. The processed liquid was coarsely filtered, ultrafiltered and desalted with a sodium filter membrane, and the ultrafiltration filtrate was evaporated in a vacuum and concentrated to 30% of the solid content. The sea cucumber flower oligopeptide was spray-dried and weighed 0.55 kg. The content of sea cucumber sulfated proteoglycan polymer (chondroitin sulfate) was measured to be 22%.

[0033] Example 5

[0034] 50 kg of chilled sea cucumber flowers were collected, soda ash was added during soaking, then they were crushed, matured, and homogenized, and 250 kg of water and 100 g of neutral protease were added, and the mixture was stirred until fully dissolved, and the enzymatic reaction was carried out at 45 ° C for 4 hours; after the enzymatic reaction, the mixture was kept warm at 90 ° C for 15 minutes to inactivate the protease; the processing liquid was coarsely filtered, ultrafiltered and desalted with a sodium filter membrane, the ultrafiltration filtrate was vacuum evaporated, concentrated to 30% of the solid content, and spray-dried to obtain sea cucumber flower oligopeptide, which weighed 0.55 kg and contained 5% of sea cucumber sulfated proteoglycan polymer (chondroitin sulfate).

[0035] The sea cucumber flower oligopeptide is mixed with a filler, a flavoring agent, a plasticizer, and a dissolving agent; wherein the filler, the flavoring agent, the plasticizer, and the dissolving agent are one or more of oligofructose, xylitol, oligomaltose, double red rose powder, grapefruit powder, sea buckthorn powder, blueberry powder, acerola powder, magnesium stearate, and alcoholic beverages; the materials are added into a rotary mixer and mixed for 20 minutes to obtain a mixed powder containing a variety of active nutrients, and the mixed powder is plasticized, encapsulated, and filled according to different dosage forms.

[0036] Sea cucumber flowers contain acidic mucopolysaccharides that have special benefits for the human body, cartilage sulfate proteoglycans (chondroitin sulfate), DHA, and active substances such as vanadium, nucleic acid, arginine, which are higher in content than sea cucumber walls. They also contain sea cucumber saponins, selenium, iron, calcium and other ingredients that can improve the body's immunity, enhance the body's disease resistance, delay aging and increase cell vitality.

[0037] The production process of the extraction method of the present invention is purely green, without acid or alkali chemical addition, and solid-state fermented liquor is added during enzymatic hydrolysis to increase the physical denaturation rate of the protein, reduce the loss of nutrients, and increase the yield of effective peptide powder.

[0038] Solid-state fermented liquor contains more than 20 ingredients, including acetaldehyde, acetal, propionaldehyde, butyraldehyde, n-butyraldehyde, isobutyraldehyde, isovaleraldehyde, hexanal, phenylacetaldehyde, isovaleraldehyde, acetone, diacetyl, and 3-hydroxybutanone. They are hydroxylated compounds that can form hydrogen bond structures with proteins, accelerate the loosening of sea cucumber and sea cucumber intestinal egg molecules, speed up enzymatic hydrolysis, and increase yields; but hydrogen bonds are not chemical bonds, and there is no chemical bond breaking during the entire change process, so that things like cartilage sulfate proteoglycans are less lost and abundant, with high overall yields, and are rich in trace elements such as calcium, iron, zinc, selenium, and alum, sea cucumber mucopolysaccharides, etc.; the ingredients in the warm enzymatic liquor are oxidized with the trimethylamine in the raw materials to form oxidized trimethylamine, so that the enzymatic solution has no fishy smell, and the dried sea cucumber flower oligopeptides have high yields, and the proportion of sea cucumber sulfate proteoglycan polymers is as high as more than 50%, which is delicious and nutritious.

[0039] The active substances in the sea cucumber flower oligopeptide prepared by the invention contain multiple nutrients, including alum, calcium, iron, zinc, selenium, sea cucumber mucopolysaccharide, sea cucumber saponin, chondroitin sulfate, etc.; the reaction conditions of the entire process are mild, no organic solvent is used, and the nutrient components are less damaged. The content of sea cucumber sulfated proteoglycan polymer (chondroitin sulfate) is as high as 50%, which is about 10 times that of other similar sea cucumber products.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for extracting active peptides from sea cucumber flowers, characterized in that: The following steps are involved: Step 1: After washing the sea cucumber flowers, soak them in water, and then ripen the soaked sea cucumber flowers with boiling water, and then crush and homogenize them to obtain a homogenate; Step 2: adding neutral protease and solid-state fermented liquor to the homogenate for enzymolysis to obtain an enzymolysis solution; Step 3: heating the enzymatic hydrolysate to inactivate the active biological protease, and then coarse filtering to obtain a coarse filtrate; Step 4: ultrafiltration and desalination of the crude filtrate to obtain an ultrafiltrate, and concentrating the ultrafiltrate to obtain a clarified liquid; Step 5: Dry the clarified liquid and pack it into bags to obtain the sea cucumber flower active peptide; In the step 2, 1% by weight of the homogenate of solid-state fermented liquor is added during enzymolysis, the temperature of the enzymolysis in step 2 is 45° C., and the duration of the enzymolysis is 4 hours; in step 3, the temperature of the enzymolysis solution is heated to 90° C., and the heating time is 15 minutes; in step 4, the crude filtrate is ultrafiltered using a sodium filter membrane.

2. The method for extracting active peptides from sea cucumber flowers according to claim 1, characterized in that: In step 1, the sea cucumber flowers are soaked in water for 2 hours.

3. The method for extracting active peptides from sea cucumber flowers according to claim 1, characterized in that: In step 5, after the clarified liquid is dried, fillers, flavoring agents, plasticizers, and dissolving agents are added, and then the liquid is packaged.

4. The method for extracting active peptides from sea cucumber flowers according to claim 3, characterized in that: The filler, flavoring agent, plasticizer and dissolving agent are one or more of oligofructose, xylitol, oligomaltose, double red rose powder, grapefruit powder, sea buckthorn powder, blueberry powder, acerola cherry powder and magnesium stearate.

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