Rapidly soluble sodium hyaluronate, preparation method, application and product

Abstract

The present invention provides a fast-soluble sodium hyaluronate, a preparation method, an application and a product, and relates to the fields of functional beverages, health products and pharmaceutical technology. The present invention adopts a spray drying process to embed sodium hyaluronate in the form of microcapsules, thereby effectively improving the fast solubility of sodium hyaluronate. Compared with single sodium hyaluronate, the prepared fast-soluble sodium hyaluronate product can be quickly dissolved in water without precipitation, powder particles coagulating into blocks, etc. The present invention selects a protein agent as an embedding wall material, which improves the solubility of sodium hyaluronate while increasing the nutritional value of the fast-soluble sodium hyaluronate, providing high-quality protein for the human body and improving human immunity.

Description

Technical Field

[0001] The invention relates to the technical fields of functional beverages, health products and medicines, and in particular to a quick-soluble sodium hyaluronate, a preparation method, an application and a product. Background Art

[0002] Hyaluronic acid is commonly known as hyaluronic acid, and its molecular formula is (C 14 H 21 NO 11 )n, is a glucuronic acid macromolecular polysaccharide composed of D-glucuronic acid and N-acetyl-D-glucosamine disaccharide. In industrial production, sodium hyaluronate (C 14 H 2 ONO 11 Sodium hyaluronate is a kind of natural hyaluronate with a strong ability to store and process. With its four unique properties of human self-origin, biocompatibility, strong water retention and viscoelastic lubricity, sodium hyaluronate has a very high application value in many fields such as medicine, food, and daily chemicals. However, the terminal market size in the food field is relatively low, and there is still a lot of room for improvement in overall maturity.

[0003] Currently, due to technical constraints, the application of related food-grade sodium hyaluronate products is rare. Sodium hyaluronate itself has excellent solubility in the human body, but during the processing, due to its strong water retention, the dissolution rate is slow, which is a difficulty restricting product production. This problem needs to be solved urgently.

[0004] At present, there is no relevant technical support for the rapid solubility of hyaluronic acid in the beverages and oral liquids on the market. Based on this, providing a microcapsule encapsulated sodium hyaluronate technology and related rapid-soluble products is of great significance to the development of sodium hyaluronate in the food field.

[0005] In view of this, the present invention is proposed. Summary of the invention

[0006] One of the purposes of the present invention is to provide an embedding method of fast-soluble sodium hyaluronate to at least solve one of the technical problems existing in the prior art.

[0007] The second object of the present invention is to provide fast-soluble sodium hyaluronate prepared by the above-mentioned embedding method.

[0008] The third object of the present invention is to provide the use of the above-mentioned fast-soluble sodium hyaluronate in preparing beverages.

[0009] A fourth object of the present invention is to provide a solid beverage.

[0010] A fifth object of the present invention is to provide a liquid beverage.

[0011] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0012] The invention provides an embedding method for fast-soluble sodium hyaluronate, comprising: adding a protein agent to a sodium hyaluronate solution, mixing uniformly and performing pretreatment, then sequentially adding a solubilizer and an emulsifier and mixing uniformly again to obtain a mixed solution, and spray-drying the mixed solution to obtain a solid powder of the fast-soluble sodium hyaluronate.

[0013] Furthermore, the molecular weight of the sodium hyaluronate is 11×10 4 ~50×10 4 Da;

[0014] Preferably, the sodium hyaluronate solution is a sodium hyaluronate aqueous solution, wherein the mass ratio of sodium hyaluronate to water is 1:90 to 110, preferably 1:100.

[0015] Further, the protein agent includes whey protein, preferably whey protein concentrate;

[0016] Preferably, the purity of the whey protein concentrate is 75% to 85%, preferably 80%;

[0017] Preferably, the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1:5-8, preferably 1:6.25.

[0018] Further, the solubilizing agent includes sucrose;

[0019] Preferably, the mass ratio of sodium hyaluronate to sucrose is 1:8-12, preferably 1:10;

[0020] The emulsifier includes at least one of sucrose ester and soy lecithin;

[0021] Preferably, the mass ratio of the sodium hyaluronate, sucrose ester and soybean lecithin is 1:1-3:1-2, preferably 1:2:1.

[0022] Furthermore, the pretreatment includes rewarming and adjusting pH after low temperature treatment;

[0023] Preferably, the pretreatment includes standing at 4°C for 6 hours, then returning to room temperature, and adjusting pH to 7.0.

[0024] Further, after adding the emulsifier, the mixture is evenly mixed by heating and stirring;

[0025] Preferably, the method further comprises the step of sterilizing the mixed solution before spray drying;

[0026] Preferably, the mixed solution is sterilized by ultra-high temperature instantaneous sterilization, cooled to room temperature and then spray-dried;

[0027] Preferably, the ultra-high temperature instantaneous sterilization heating temperature is set to 130-150°C, and the heating time is 5-15 seconds;

[0028] Preferably, the spray drying parameters are set as follows: air inlet temperature 170-180°C, air outlet temperature 80-105°C.

[0029] The present invention also provides fast-soluble sodium hyaluronate prepared by the embedding method of the fast-soluble sodium hyaluronate.

[0030] The present invention also provides the use of the above-mentioned fast-soluble sodium hyaluronate in preparing beverages;

[0031] Preferably, the beverage includes a solid beverage and a liquid beverage;

[0032] Preferably, the solid beverage comprises instant milk tea and protein powder; and the liquid beverage comprises a sports drink.

[0033] The present invention also provides a solid beverage, comprising the instant soluble sodium hyaluronate and a main ingredient acceptable to food;

[0034] Preferably, the mass ratio of the instant sodium hyaluronate to the main material is 1:5.65-5.67;

[0035] Preferably, the main ingredient includes milk tea powder or protein powder.

[0036] The present invention also provides a liquid beverage, comprising the above-mentioned fast-soluble sodium hyaluronate and a food-acceptable auxiliary material;

[0037] Preferably, the auxiliary materials include distilled water, sweeteners and nutritional extracts;

[0038] Preferably, the sweetener comprises one or more of mogroside, trehalose and erythritol; the nutritional extract comprises cranberry extract, atractylodes macrocephala extract and β-carotene;

[0039] Preferably, the amount of the instant sodium hyaluronate and mogroside added is 0.15-0.5% of the amount of sucrose used; and / or, the amount of trehalose added is 5-15% of the amount of sucrose used; and / or, the amount of erythritol added is 5-12% of the amount of sucrose used; the mass ratio of mogroside, trehalose and erythritol is 0.013:6:1.2;

[0040] Preferably, the mass ratio of the fast-soluble sodium hyaluronate to the cranberry extract is 1:2-8;

[0041] Preferably, the mass ratio of the fast-soluble sodium hyaluronate to the Atractylodes macrocephala extract is 1:0.05-0.3;

[0042] Preferably, the mass ratio of the fast-soluble sodium hyaluronate to β-carotene is 1:0.1-0.5.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] 1. The present invention adopts a spray drying process to embed sodium hyaluronate in the form of microcapsules, which effectively improves the rapid solubility of sodium hyaluronate. Compared with single sodium hyaluronate, the prepared rapid-soluble sodium hyaluronate product can be quickly dissolved in water without precipitation, powder particles coagulating into lumps, etc.

[0045] 2. The present invention uses protein agents as embedding wall materials to improve the solubility of sodium hyaluronate while increasing the nutritional value of fast-soluble sodium hyaluronate, providing high-quality protein for the human body and improving human immunity.

[0046] 3. The present invention adds sodium hyaluronate to milk tea, solid protein drinks, and sports drinks, which have the effects of moisturizing, improving skin condition, strengthening joints, etc. DETAILED DESCRIPTION

[0047] Unless otherwise defined herein, scientific and technical terms used in conjunction with the present invention shall have the meanings commonly understood by those of ordinary skill in the art. The meaning and scope of the terms should be clear, however, in the case of any potential ambiguity, the definitions provided herein take precedence over any dictionary or external definitions. In this application, unless otherwise stated, the use of "or" means "and / or". In addition, the use of the term "including" and other forms is non-limiting.

[0048] Generally, the nomenclature and its technology used in conjunction with cell and tissue culture, molecular biology, immunology, microbiology, genetics, and protein and nucleic acid chemistry and hybridization described herein are those well-known and commonly used in the art. Unless otherwise indicated, the methods and techniques of the present invention are generally carried out according to conventional methods as well-known in the art, and as described in various general and more specific references, which are cited and discussed throughout this specification. Enzymatic reactions and purification techniques are carried out according to the manufacturer's specifications, as generally achieved in the art, or as described herein. The nomenclature and its laboratory procedures and technology used in conjunction with analytical chemistry, synthetic organic chemistry, and medical and pharmaceutical chemistry described herein are those well-known and commonly used in the art.

[0049] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] Microcapsule embedding technology uses various natural or synthetic polymer compounds as the external phase to completely encapsulate the target object without changing the original chemical properties of the target object. Microcapsule embedding technology can improve the stability of the product, prevent mutual interference between components, and can also change the physical properties of the core material (color, shape, density, dispersion performance). In the food field, some substances can be integrated into the food system and maintain physiological activity. After years of development, microcapsule embedding technology has existing spray drying, physical and chemical methods, interfacial polymerization methods, etc., among which spray drying is the most mature and widely used in the food field. The present invention realizes microcapsule embedding of sodium hyaluronate by spray drying. Specifically, according to one aspect of the present invention, a method for embedding fast-soluble sodium hyaluronate is provided, comprising: adding a protein agent to a sodium hyaluronate solution, pre-treating after mixing evenly, then adding a solubilizer and an emulsifier in sequence and mixing evenly again to obtain a mixed solution, spray drying the mixed solution to obtain a solid powder of the fast-soluble sodium hyaluronate.

[0051] The embedding method provided by the present invention has a simple process and is easy to operate. The fast-soluble sodium hyaluronate prepared by the method provided by the present invention effectively improves the fast solubility of sodium hyaluronate. Compared with single sodium hyaluronate, it can be quickly dissolved in water without precipitation, powder particles coagulating into blocks, etc. In addition, the present invention selects a protein agent as an embedding wall material, which improves the solubility of sodium hyaluronate and increases the nutritional value of the fast-soluble sodium hyaluronate, providing high-quality protein for the human body and improving human immunity.

[0052] In some preferred embodiments, the molecular weight of the sodium hyaluronate is 11×10 4 ~50×10 4 Da, for example, can be, but is not limited to, 11×10 4 Da, 20×10 4 Da, 30×10 4 Da, 40×10 4 Da or 50×10 4 Da.

[0053] Preferably, the sodium hyaluronate solution is an aqueous solution of sodium hyaluronate, wherein the mass ratio of sodium hyaluronate to water is 1:90-110, for example, but not limited to 1:90, 1:95, 1:100, 1:105 or 1:110. If the concentration is too high, the liquid will be too viscous and will clog the pipeline, and if it is too dilute, it will waste industrial production resources. It is preferably 1:100.

[0054] In some preferred embodiments, the protein agent includes whey protein, which is a fast-soluble whey protein and has good fast solubility. For the convenience of industrial application, it is preferably whey protein concentrate;

[0055] Preferably, the purity of the whey protein concentrate is 75% to 85%, preferably 80%; when the purity of the whey protein concentrate is too low, the encapsulation rate is low and the raw materials are wasted; when the concentration of the whey protein concentrate is too high, the whey protein produces gel and the powder spraying operation cannot be performed.

[0056] Preferably, the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1:5-8, for example, it can be, but not limited to, 1:5, 1:6, 1:7 or 1:8, preferably 1:6.25.

[0057] In some preferred embodiments, the solubilizing agent includes sucrose; using sucrose as the solubilizing agent of the present invention can increase the solubility of the solution on the one hand, and can also increase the taste of the sweet and rich product on the other hand.

[0058] The emulsifier in the present invention can reduce the electrostatic repulsion between whey protein and polysaccharide, so as to make the particle size uniform, and the emulsifier includes at least one of sucrose ester and soy lecithin. Typical emulsifiers can select sucrose ester alone, soy lecithin alone, or both sucrose ester and soy lecithin. When all are selected, the emulsifier used in the present invention is more helpful to change the surface hydrophobicity of whey protein concentrate and hyaluronic acid.

[0059] Preferably, the mass ratio of sodium hyaluronate to sucrose is 1:8-12, for example, it can be, but not limited to, 1:8, 1:9, 1:10, 1:11 or 1:12, preferably 1:10;

[0060] Preferably, the mass ratio of the sodium hyaluronate, sucrose ester and soybean lecithin is 1:1-3:1-2, for example, it can be, but not limited to, 1:1:1, 1:3:2, 1:3:1 or 1:1:2, preferably 1:2:1.

[0061] In some preferred embodiments, the pretreatment includes rewarming after low-temperature treatment and adjusting the pH; the treatment method of rewarming after low-temperature treatment can further fully hydrate the protein and hyaluronic acid and completely dissolve them.

[0062] Preferably, the pretreatment includes resting the mixture at 4°C for 6 hours and then restoring the mixture to room temperature, and adjusting the pH to 7.0, wherein the room temperature refers to 20-25°C.

[0063] In some preferred embodiments, after the emulsifier is added, the mixture is heated and stirred to be uniformly mixed; wherein, the container is kept sealed during the heating and stirring to avoid evaporation of water, which leads to an increase in the total solid content ratio and the formation of gel, for example, a plastic wrap can be used to cover the container mouth.

[0064] Preferably, the mixed solution is sterilized before spray drying; preferably, the mixed solution is sterilized at ultra-high temperature instantaneously, and spray dried after cooling to room temperature, wherein stirring is continued during the heating process.

[0065] Among them, the ultra-high temperature instantaneous sterilization heating temperature is set to 130-150°C, and the heating time is 5-15 seconds; the spray drying parameters are set to: air inlet temperature 170-180°C, air outlet temperature 80-105°C.

[0066] According to a second aspect of the present invention, there is also provided fast-soluble sodium hyaluronate prepared by the above-mentioned fast-soluble sodium hyaluronate embedding method.

[0067] The fast-soluble sodium hyaluronate provided by the present invention can be quickly dissolved in water without precipitation or powder particles coagulating into lumps, etc., and the fast-soluble sodium hyaluronate also has high nutritional value, can provide high-quality protein to the human body, and improve human immunity.

[0068] Based on the beneficial effects of the fast-soluble sodium hyaluronate provided by the present invention, according to the third aspect of the present invention, the present invention also provides the use of the fast-soluble sodium hyaluronate in preparing beverages.

[0069] The present invention adds sodium hyaluronate to milk tea, solid protein beverages, sports drinks, etc., which has the effects of replenishing water and moisturizing, improving skin condition, strengthening joints, etc.

[0070] The beverage can be a solid beverage or a liquid beverage. Specifically, the solid beverage is a powder, packaged in small bags, such as, but not limited to, instant milk tea and protein powder; the liquid beverage is a solution, not exceeding 250 ml, bottled, such as, but not limited to, sports drinks.

[0071] It can be understood that based on the fast-dissolving property of the fast-dissolving sodium hyaluronate provided by the present invention, it is preferably applied to the beverage field. In addition, it can also be applied to the fields of food or medicine.

[0072] As a relatively specific embodiment, the solid beverage provided by the present invention comprises the instant soluble sodium hyaluronate and a main ingredient acceptable to food;

[0073] Preferably, the mass ratio of the instant sodium hyaluronate to the main material is 1:5.65-5.67;

[0074] Preferably, the main ingredient includes milk tea powder or protein powder.

[0075] As a relatively specific embodiment, the liquid beverage provided by the present invention comprises the above-mentioned fast-soluble sodium hyaluronate and a food-acceptable auxiliary material;

[0076] Preferably, the auxiliary materials include distilled water, sweeteners and nutritional extracts;

[0077] Preferably, the sweetener comprises one or more of mogroside, trehalose and erythritol; the nutritional extract comprises cranberry extract, atractylodes macrocephala extract and β-carotene;

[0078] When 50% purity mogroside, 45% sucrose sweetened trehalose and 55% sucrose sweetened erythritol are selected, preferably, the amount of the instant sodium hyaluronate and mogroside added is 0.15-0.5% of the amount of sucrose used; and / or, the amount of the trehalose added is 5-15% of the amount of sucrose used; and / or, the amount of the erythritol added is 5-12% of the amount of sucrose used; the mass ratio of mogroside, trehalose and erythritol is 0.013:6:1.2;

[0079] Preferably, the mass ratio of the fast-soluble sodium hyaluronate to the cranberry extract is 1:2-8;

[0080] Preferably, the mass ratio of the fast-soluble sodium hyaluronate to the Atractylodes macrocephala extract is 1:0.05-0.3;

[0081] Preferably, the mass ratio of the fast-soluble sodium hyaluronate to β-carotene is 1:0.1-0.5.

[0082] It should be noted that, since the human body should not consume more than 0.2 grams of sodium hyaluronate per day, the instant milk tea, protein powder or sports drink provided by the present invention contains no more than 0.2 grams of sodium hyaluronate per bag or bottle. The present invention controls the intake of sodium hyaluronate within a reasonable range, not exceeding 0.2 grams per day, and exerts the nutritional and health care effects under the premise of ensuring safety.

[0083] In addition, the present invention also provides a method for preparing the above-mentioned solid beverage or liquid beverage, which comprises mixing a formula amount of fast-soluble sodium hyaluronate with a main ingredient or auxiliary ingredient acceptable to food to obtain a solid beverage or liquid beverage.

[0084] The present invention is further described below by way of examples. Unless otherwise specified, the materials in the examples are prepared according to existing methods or directly purchased from the market.

[0085] Example 1

[0086] This embodiment provides an embedding method of fast-soluble sodium hyaluronate, comprising the following steps:

[0087] (1) Dissolution: Use a molecular weight of 11×10 4 Da of sodium hyaluronate is added to distilled water and slowly dissolved, and then whey protein concentrate with a purity of 80% is added and stirred until completely dissolved. The mass ratio of sodium hyaluronate to distilled water is 1:100, and the mass ratio of sodium hyaluronate to whey protein concentrate is 1:6.25;

[0088] (2) Hydration: The mixed solution obtained in (1) was refrigerated at 4°C for 6 hours;

[0089] (3) Adjusting pH: Take the refrigerated solution in (2), return it to room temperature, and adjust pH = 7.0;

[0090] (4) Add reagents: add sucrose, sucrose ester, and soy lecithin. The mass ratio of sodium hyaluronate to sucrose is 1:10; the mass ratio of sodium hyaluronate, sucrose ester, and soy lecithin is 1:2:1. Then cover the bottle mouth with plastic wrap and heat and stir;

[0091] (5) Ultra-high temperature instantaneous sterilization: The heating temperature is set to 130-150°C, the heating time is 5-15 seconds, stirring continuously during the heating process, and then cooling to room temperature;

[0092] (6) Spray drying: The mixed solution is sprayed with powder, and the ultrafine powder sprayed out is the fast-soluble sodium hyaluronate.

[0093] Example 2

[0094] This embodiment provides an embedding method of fast-soluble sodium hyaluronate, comprising the following steps:

[0095] (1) Dissolution: Use a molecular weight of 50×10 4 Da of sodium hyaluronate is added to distilled water and slowly dissolved, and then whey protein concentrate with a purity of 75% is added and stirred until completely dissolved. The mass ratio of sodium hyaluronate to distilled water is 1:110, and the mass ratio of sodium hyaluronate to whey protein concentrate is 1:5;

[0096] (2) Hydration: The mixed solution obtained in (1) was refrigerated at 4°C for 6 hours;

[0097] (3) Adjusting pH: Take the refrigerated solution in (2), return it to room temperature, and adjust pH = 7.0;

[0098] (4) Add reagents: add sucrose, sucrose ester, and soy lecithin. The mass ratio of sodium hyaluronate to sucrose is 1:8; the mass ratio of sodium hyaluronate, sucrose ester, and soy lecithin is 1:3:2. Then cover the bottle mouth with plastic wrap and heat and stir;

[0099] (5) Ultra-high temperature instantaneous sterilization: The heating temperature is set to 130-150°C, the heating time is 5-15 seconds, stirring continuously during the heating process, and then cooling to room temperature;

[0100] (6) Spray drying: The mixed solution is sprayed with powder, and the ultrafine powder sprayed out is the fast-soluble sodium hyaluronate.

[0101] Example 3

[0102] This embodiment provides an embedding method of fast-soluble sodium hyaluronate, comprising the following steps:

[0103] (1) Dissolution: Use a molecular weight of 35×10 4 Da of sodium hyaluronate is added to distilled water and slowly dissolved, and then whey protein concentrate with a purity of 85% is added and stirred until completely dissolved. The mass ratio of sodium hyaluronate to distilled water is 1:90, and the mass ratio of sodium hyaluronate to whey protein concentrate is 1:8;

[0104] (2) Hydration: The mixed solution obtained in (1) was refrigerated at 4°C for 6 hours;

[0105] (3) Adjusting pH: Take the refrigerated solution in (2), return it to room temperature, and adjust pH = 7.0;

[0106] (4) Add reagents: add sucrose, sucrose ester, and soy lecithin. The mass ratio of sodium hyaluronate to sucrose is 1:12; the mass ratio of sodium hyaluronate, sucrose ester, and soy lecithin is 1:1:1. Then cover the mouth of the container with plastic wrap and heat and stir.

[0107] (5) Ultra-high temperature instantaneous sterilization: The heating temperature is set to 130-150°C, the heating time is 5-15 seconds, stirring continuously during the heating process, and then cooling to room temperature;

[0108] (6) Spray drying: The mixed solution is sprayed with powder, and the ultrafine powder sprayed out is the fast-soluble sodium hyaluronate.

[0109] Comparative Example 1

[0110] This comparative example provides an embedding method for fast-soluble sodium hyaluronate, which is different from Example 1 in that the molecular weight of the sodium hyaluronate is 55×10 4 Da.

[0111] Comparative Example 2

[0112] This comparative example provides an embedding method for fast-soluble sodium hyaluronate, which is different from Example 1 in that the mass ratio of the sodium hyaluronate to distilled water is 1:80.

[0113] Comparative Example 3

[0114] This comparative example provides an embedding method for fast-soluble sodium hyaluronate, which is different from Example 1 in that the mass ratio of the sodium hyaluronate to sucrose is 1:6.

[0115] Comparative Example 4

[0116] This comparative example provides an embedding method of fast-soluble sodium hyaluronate, which is different from Example 1 in that the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1:10.

[0117] Comparative Example 5

[0118] This comparative example provides an embedding method for fast-soluble sodium hyaluronate, which differs from Example 1 in that step (2) is not included.

[0119] Experimental example

[0120] The fast-soluble sodium hyaluronate prepared by the embedding method of the fast-soluble sodium hyaluronate provided in Examples 1-3 and Comparative Examples 1-5 was tested for dissolution performance, and the test methods were water absorption index, water solubility index, Zeta potential, stability, dispersibility and solubility.

[0121] Determination method of water absorption index and water solubility index: accurately weigh 2.0g of the product, place it in a centrifuge tube with known constant weight, add 25mL of distilled water and shake until the product powder is completely dispersed in the water. Keep warm in a 30℃ water bath for 30min, and shake once every 10min. Centrifuge at 4000r / min for 30min, take the supernatant, pour it into a plate with known constant weight, and evaporate to dryness in an oven at 105℃ to constant weight. The water solubility index and water absorption index are calculated as follows:

[0122]

[0123]

[0124] Where: WSI is the water solubility index; WAI is the water absorption index; W0 is the mass of the raw material powder, g; W 1 is the mass of the centrifuge tube, g; W 2 is the mass of the plate, g; W 3 is the sum of the mass of the plate and the supernatant, g; W 4 is the sum of the mass of the centrifuge tube and the precipitate, g.

[0125] Zeta potential: measured using laser Doppler electrophoresis light scattering technology. The temperature was set at 25°C. The sample solution diluted 100 times was injected into an electrophoretic cell with voltage applied at both ends. The Zeta potential was calculated by directly integrating the experimentally determined electrophoretic mobility using the Helmholtz-Smoluchowski equation.

[0126] Stability and dispersibility: Take 0.625g powder and add 5mL deionized water to dissolve, warm it in a 40℃ water bath for 10min, and then vortex it for 2min to completely dissolve the milk powder. Take the emulsion and add it dropwise to the injection port of the laser diffraction particle size analyzer. The amount of dropwise addition should make the refractive index of the sample between 10% and 15%. Each sample is measured three times and the average value is taken.

[0127] The solubility determination method refers to the national standard GB5413.29-2010 National Food Safety Standard Determination of Solubility of Infant Food and Dairy Products.

[0128] The experimental results are shown in the following table:

[0129] Group Water absorption index Water solubility index Zeta Potential stability Dispersibility Solubility Example 1 188.05 72.76 -16.84 0.03081 0.8313 99.8407 Example 2 182.62 71.52 -17.192 0.04668 0.6844 99.8789 Example 3 187.37 73.21 -21.56 0.03484 0.5885 99.5179 Comparative Example 1 191.15 73.25 -10.25 0.02458 0.5485 95.244 Comparative Example 2 145.32 56.22 -8.65 0.02415 0.5785 96.2584 Comparative Example 3 153.24 45.85 -7.54 0.01532 0.6215 97.2545 Comparative Example 4 180.52 57.22 -8.25 0.01475 0.6127 95.2257 Comparative Example 5 135.55 45.21 -5.63 0.01658 0.5483 93.2458

[0130] It can be seen from the above table that the molecular weight of sodium hyaluronate, the ratio of sodium hyaluronate to distilled water, sucrose, and whey protein concentrate, and the hydration steps all have an impact on the production of instant sodium hyaluronate; from the perspective of cost saving, the instant sodium hyaluronate prepared in Example 1 with the better effect is applied to subsequent examples in the present invention.

[0131] Example 4 Preparation of instant milk tea

[0132] The instant sodium hyaluronate provided in Example 1 of the present invention and the milk tea powder are homogeneously mixed at a mass ratio of 1:5.67 to obtain a product powder, which is packaged into small bags of 15 grams. When preparing, 200 ml of hot water at about 80° C. is added to each small bag.

[0133] Example 5 Preparation of protein powder

[0134] The instant hyaluronate provided in Example 1 of the present invention and the protein powder are homogeneously mixed at a mass ratio of 1:5.67 to obtain a product powder, which is packaged into small bags of 15 grams. When preparing, 200 ml of hot water at about 80° C. is added to each small bag.

[0135] Example 6 Preparation of sports drink

[0136] The instant hyaluronate provided in Example 1 of the present invention, mogroside (Hunan Huacheng Biotechnology Co., Ltd.), trehalose (Japan Nagase Foods Co., Ltd.) and erythritol (Shandong Baolingbao Biological Co., Ltd.), cranberry extract (Shaanxi Xinyanghe Biotechnology Co., Ltd.), Atractylodes macrocephala extract (Lanzhou Waters), and β-carotene (Lanzhou Waters) were mixed and dissolved in distilled water to prepare a sports drink. The amount of mogroside added is 0.15-0.5% of the amount of sucrose, the amount of trehalose added is 5-15% of the amount of sucrose, and the amount of erythritol added is 5-12% of the amount of sucrose, wherein the mass ratio of mogroside, trehalose and erythritol is ensured to be 0.013:6:1.2; the mass ratio of instant sodium hyaluronate to cranberry extract is 1:2-8, the mass ratio of instant sodium hyaluronate to Atractylodes macrocephala extract is 1:0.05-0.3, the mass ratio of instant sodium hyaluronate to beta-carotene is 1:0.1-0.5, and the total volume of each portion does not exceed 250ml.

[0137] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for embedding fast-soluble sodium hyaluronate, It is characterized in that include: Adding a protein agent to a sodium hyaluronate solution, mixing well and performing pretreatment, then adding a solubilizing agent and an emulsifier and mixing well again to obtain a mixed solution, spray drying the mixed solution to obtain a solid powder of the instant sodium hyaluronate; The molecular weight of the sodium hyaluronate is 11×10 4 ~50×10 4 Da; The sodium hyaluronate solution is a sodium hyaluronate aqueous solution, wherein the mass ratio of sodium hyaluronate to water is 1:90-110; The protein agent includes whey protein concentrate; The purity of the whey protein concentrate is 75% to 85%; The mass ratio of sodium hyaluronate to whey protein concentrate is 1:5-8; The dissolution agent includes sucrose; The mass ratio of sodium hyaluronate to sucrose is 1:8~12; The pretreatment includes standing at 4°C for 6 hours, returning to room temperature, and adjusting the pH to 7.0; The emulsifier includes at least one of sucrose ester and soy lecithin; The mass ratio of the sodium hyaluronate, sucrose ester and soybean lecithin is 1:1-3:1-2.

2. The embedding method according to claim 1, It is characterized in that The mass ratio of sodium hyaluronate to water in the sodium hyaluronate aqueous solution is 1:

100.

3. The embedding method according to claim 1, It is characterized in that The purity of the whey protein concentrate is 80%; The mass ratio of the sodium hyaluronate to the whey protein concentrate is 1:6.

25.

4. The embedding method according to claim 1, It is characterized in that The mass ratio of the sodium hyaluronate to sucrose is 1:

10.

5. The embedding method according to claim 4, It is characterized in that The mass ratio of the sodium hyaluronate, sucrose ester and soybean lecithin is 1:2:

1.

6. The embedding method according to claim 1, It is characterized in that After adding the emulsifier, mix evenly by heating and stirring; The method also includes the step of sterilizing the mixed solution before spray drying; The mixed solution is subjected to ultra-high temperature instant sterilization, and spray-dried after being cooled to room temperature; The ultra-high temperature instantaneous sterilization heating temperature is set to 130-150°C, and the heating time is 5-15 seconds; The spray drying parameters were set as follows: air inlet temperature 170~180℃, air outlet temperature 80~105℃.

7. Rapidly soluble sodium hyaluronate prepared by the embedding method of rapidly soluble sodium hyaluronate according to any one of claims 1 to 6.

8. Use of the fast-soluble sodium hyaluronate according to claim 7 in preparing beverages.

9. The use according to claim 8, It is characterized in that The beverages include solid beverages and liquid beverages; The solid beverage includes instant milk tea and protein powder; the liquid beverage includes sports drink.

10. A solid beverage, It is characterized in that The invention comprises the fast-soluble sodium hyaluronate as claimed in claim 7 and a main ingredient acceptable to food.

11. The solid beverage according to claim 10, It is characterized in that The mass ratio of the instant sodium hyaluronate to the main material is 1:5.65-5.67; The main ingredients include milk tea powder or protein powder.

12. A liquid beverage, It is characterized in that The invention comprises the fast-soluble sodium hyaluronate as claimed in claim 7 and a food-acceptable auxiliary material.

13. The liquid beverage according to claim 12, It is characterized in that The auxiliary materials include distilled water, sweeteners and nutritional extracts; The sweetener includes one or more of mogroside, trehalose and erythritol; the nutritional extract includes cranberry extract, atractylodes macrocephala extract and beta-carotene; The amount of mogroside added is 0.15-0.5% of the amount of sucrose; and / or the amount of trehalose added is 5-15% of the amount of sucrose; and / or the amount of erythritol added is 5-12% of the amount of sucrose; the mass ratio of mogroside, trehalose and erythritol is 0.013:6:1.2; The mass ratio of the instant sodium hyaluronate to the cranberry extract is 1:2-8; The mass ratio of the instant sodium hyaluronate to the Atractylodes macrocephala extract is 1:0.05-0.3; The mass ratio of the fast-soluble sodium hyaluronate to beta-carotene is 1:0.1-0.5.

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