Method for improving quality of reconstituted tobacco leaves

Abstract

The present invention discloses a method for improving the quality of reconstituted tobacco, comprising the following steps: picking a single colony of Pseudomonas paraflavonoids ZY-03 with a Tibetan number of CGMCC NO.24422 and inoculating it into a liquid culture medium to obtain a seed liquid; centrifuging the seed liquid to remove the supernatant, adding sterile water to redissolve it, preparing a bacterial suspension, adding a protective agent, vacuum drying, and preparing bacterial powder; adding bacterial powder to a papermaking pulp, and then fermenting it to form a substrate; adding bacterial powder to a tobacco extract, and then concentrating it after fermentation to form a coating liquid; and drying it after coating. This application combines the process of reconstituted tobacco itself, uses Pseudomonas paraflavonoids ZY-03 to prepare a bacterial agent dry powder, and adds the bacterial powder to the papermaking pulp and tobacco extract respectively for fermentation, efficiently degrading lignin. At the same time, the rich enzymes produced by the strain have a certain flavoring effect, are sterilized in the drying stage, reduce the lignin in the reconstituted tobacco substrate, and increase the aroma of the coating liquid to improve the quality of reconstituted tobacco.

Description

Technical Field

[0001] The present invention relates to the technical field of cigarette raw material production and processing, and in particular to a method for improving the quality of reconstituted tobacco leaves. Background Art

[0002] Reconstituted tobacco, also known as tobacco flakes, is an important form of tobacco waste recycling, with great environmental and economic value. Its production technologies mainly include roller pressing, thick pulping, and papermaking. The typical production process for papermaking-based reconstituted tobacco involves extracting tobacco raw materials with water and performing solid-liquid separation to obtain tobacco extract and tobacco insolubles. The tobacco extract is then refined, concentrated, flavored, and made into a coating liquid. The tobacco insolubles are then pulped and papered into tobacco base sheets. The coating liquid and tobacco base sheets are then recombined through dipping or spraying, and finally dried and cut into pieces to produce the finished product.

[0003] The raw materials for reconstituted tobacco leaves used in papermaking come from the following sources: (1) tobacco leaves, axillary buds, tobacco stems, etc. other than fresh tobacco leaves during tobacco harvesting; (2) low-grade tobacco leaves that cannot be processed into cigarettes during tobacco production, drying, redrying and cigarette production and processing, as well as a large amount of upper tobacco leaves, tobacco dust, tobacco shreds and tobacco stalks; (3) debris and tobacco dust generated during tobacco processing, as well as separated tobacco stems that account for 23-25% of the weight of tobacco leaves; (4) fiber materials in wood pulp are added to reconstituted tobacco raw materials to increase their fiber content; (5) product recycling. Finished products collected from distribution channels are then recycled to the factory for further processing.

[0004] Since papermaking-based reconstituted tobacco is made from a large number of by-products generated during cigarette production, as well as wood pulp and hemp pulp, the leaf matrix of reconstituted tobacco is mostly composed of supporting substances such as cellulose and lignin. Many of these macromolecular substances are also present in reconstituted tobacco concentrate, which not only affects the coating effect but also has a negative impact on the smoking quality of the reconstituted tobacco.

[0005] As we all know, lignin is a complex natural polymer compound second only to cellulose in content in nature and is widely present in the cell walls of higher plants. Lignin in tobacco plant cell walls is a complex three-dimensional network composed of phenylpropane derivative monomers (such as coumarin, coniferyl, and sinapyl alcohol). This substance is an amorphous and bio-polymorphic biomacromolecule. Pyrolysis of lignin primarily produces phenolic compounds. Gas chromatography-mass spectrometry (GC-MS) analysis of the pyrolysis products revealed that the phenolic content can reach 60%. Phenol, a significant harmful component in mainstream cigarette smoke, has long been included in the Canadian government's list of 46 harmful components and the list of regulated ingredients recommended by the WHO's "Tobacco Product Regulation Study Group." It enters the smoke directly during tobacco combustion, producing an unpleasant odor that is difficult to mask. It is particularly corrosive to the respiratory system and has carcinogenic effects.

[0006] Research has shown that as the lignin content in reconstituted tobacco raw materials increases, the amount of phenol released during combustion also increases. The two levels show a strong correlation: higher lignin content in the reconstituted tobacco raw materials is associated with higher levels of phenol and phenolic compounds in the pyrolysis products. At 600°C, phenol and phenolic compound contents increased by 49.3% and 39.8%, respectively, while at 800°C, phenol and phenolic compound contents increased by 28.4% and 65.1%, respectively. Furthermore, phenolic compounds such as catechol and alkylcatechols, produced during lignin pyrolysis, enter the flue gas directly, causing a harsh taste and promoting cancer, seriously impacting the quality of reconstituted tobacco. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to provide a method for improving the quality of reconstituted tobacco leaves, sterilizing in the drying stage, reducing the lignin in the reconstituted tobacco leaf base sheet, and increasing the aroma of the coating liquid to improve the quality of the reconstituted tobacco leaves.

[0008] The technical problem to be solved by the present invention is achieved through the following technical solutions:

[0009] A method for improving the quality of reconstituted tobacco leaves, comprising the following steps:

[0010] S1: A single colony of Pseudomonas paraxanthinosus ZY-03 with the accession number CGMCC NO. 24422 was inoculated into YPD liquid medium and cultured at 22-37°C and a shaking speed of 100-250 rpm for 12-72 hours to obtain seed solution.

[0011] S2: Centrifuge the seed solution at 8000-12000 r / min at 4°C for 10-20 min, remove the supernatant, add sterile water to reconstitute, and prepare a strain concentration of (1-2) × 10 9 A bacterial suspension of 100 CFU / mL was added with 1-3% glycerol, 1-5% sorbitol, 1-3% sodium ascorbate, and 2%-5% glucose as a protective agent, pre-frozen at -80°C for 8-12 hours, and vacuum-dried at -50°C, 0.4-2 Pa, to prepare bacterial powder;

[0012] S3: According to the reconstituted tobacco preparation process, tobacco stems, tobacco dust or tobacco leaf fragments are extracted with water, and then solid-liquid separation is performed. The solids are then beaten and mixed with wood pulp to form paper pulp. 2-10% bacterial powder is added to the paper pulp, and the paper pulp is fermented at 30-40°C for 12-48 hours before being papered to form a reconstituted tobacco base sheet.

[0013] S4: According to the reconstituted tobacco preparation process, tobacco stems, tobacco dust or tobacco leaf fragments are subjected to water extraction and solid-liquid separation, and the liquid is called tobacco extract; 2-8% bacterial powder is added to the tobacco extract, and the extract is fermented at 30-40°C for 12-48 hours and then concentrated to form a coating liquid;

[0014] S5: According to the reconstituted tobacco preparation process, the coating liquid is evenly coated on the reconstituted tobacco base sheet, and then dried at 80-120° C. After coating, the moisture content of the dried reconstituted tobacco is 10-13%.

[0015] Preferably, in the above technical solution, the YPD liquid culture medium is prepared by high-pressure sterilization at 121° C. for 15 minutes using 10 g / L yeast powder, 20 g / L peptone and 20 g / L glucose.

[0016] Preferably, in the above technical solution, in S2, the bacterial concentration in the bacterial powder is determined to be (2-8)×10 8 CFU / g.

[0017] Preferably, in the above technical solution, S3 is to add 6% bacterial powder to the papermaking pulp, ferment it at 37° C. for 48 hours, and then make paper to form a base sheet.

[0018] Preferably, in the above technical solution, 4% bacterial powder is added to the tobacco extract, fermented at 37° C. for 12 hours, and then concentrated to form a coating liquid.

[0019] Preferably, in the above technical solution, in S5, the drying temperature is 80° C. and the moisture content is 12%.

[0020] A reconstituted tobacco leaf is prepared by utilizing a method for improving the quality of the reconstituted tobacco leaf.

[0021] The invention discloses an application of reconstituted tobacco leaves in improving tobacco quality.

[0022] Preferably, in the above technical solution, the processed reconstituted tobacco leaves are cut into shreds and added to the formulated tobacco. After the treatment, the aroma volume and quality are improved, the softness, impurities and stimulation are significantly improved, and the overall sensory quality is improved.

[0023] The above technical solution of the present invention has the following beneficial effects:

[0024] This application combines the process of reconstituted tobacco leaves itself, uses Pseudomonas paraflavonoids ZY-03 to prepare dry powder of the bacterial agent, and adds the bacterial powder to paper pulp and tobacco extract for fermentation respectively, which efficiently degrades lignin. At the same time, the rich enzymes produced by the strain have a certain flavor-enhancing effect, sterilize during the drying stage, reduce the lignin of the reconstituted tobacco leaf base sheet, and increase the aroma of the coating liquid, so as to improve the quality of the reconstituted tobacco leaves. DETAILED DESCRIPTION

[0025] Various exemplary embodiments of the present invention will now be described in detail. It should be noted that the relative arrangement of components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless otherwise specifically stated.

[0026] The tobacco materials of this application are obtained as follows: the tobacco raw materials are obtained from a cigarette factory affiliated to Henan China Tobacco Industrial Co., Ltd. and provided by Henan China Tobacco Co., Ltd.

[0027] The Pseudomonas parafulva ZY-03 of this application is deposited in the General Biological Center of the China Microorganism Culture Collection Administration (CGMCC), with the deposit number CGMCC No. 24422 and the deposit time being February 23, 2022.

[0028] Example 1

[0029] (1) Prepare YPD medium (yeast powder 10 g / L, peptone 20 g / L, glucose 20 g / L) and sterilize by autoclaving at 121°C for 15 min.

[0030] (2) A single colony of Pseudomonas paraflavonoids ZY-03 with the accession number CGMCC NO. 24422 was selected and inoculated into YPD liquid culture medium and cultured at 30°C and a shaking speed of 120 r / min for 48 h.

[0031] (3) The seed solution was centrifuged at 12000 r / min and 4°C for 10 min, the supernatant was removed, and sterile water was added to reconstitute the solution to prepare a strain concentration of 1×10 9 CFU / mL bacterial suspension was added with 3% glycerol, 3% sorbitol, 2% sodium ascorbate, and 5% glucose as protective agents, pre-frozen at -80°C for 8 h, and vacuum-dried at -50°C, 0.7 Pa to prepare bacterial powder. The bacterial concentration in the powder was determined by the dilution plate method, and the concentration should be between 6×10 8 CFU / g.

[0032] (4) Add 8% bacterial powder to the papermaking pulp, ferment at 30°C for 48 hours, and then papermake to form a substrate.

[0033] (5) Add 3% bacterial powder to the tobacco extract, ferment at 30°C for 24 hours, and then concentrate to form a coating solution.

[0034] (6) According to the reconstituted tobacco leaf preparation process, the coating is carried out, and then the reconstituted tobacco leaf is dried at 110° C., and the sterilization effect can be achieved during the drying process. The moisture content of the dried reconstituted tobacco leaf is 12%.

[0035] Example 2

[0036] (1) Prepare YPD medium (yeast powder 10 g / L, peptone 20 g / L, glucose 20 g / L) and sterilize by autoclaving at 121°C for 15 min.

[0037] (2) A single colony of Pseudomonas paraflavonoids ZY-03 with the accession number CGMCC NO. 24422 was selected and inoculated into YPD liquid culture medium and cultured at 30°C and a shaking speed of 120 r / min for 48 h.

[0038] (3) The seed solution was centrifuged at 8000 r / min and 4°C for 10 min, the supernatant was removed, and sterile water was added to reconstitute the solution to prepare a strain concentration of 1×10 9 CFU / mL bacterial suspension was added with 2% glycerol, 5% sorbitol, 2% sodium ascorbate, and 3% glucose as protective agents, pre-frozen at -80°C for 10 h, and vacuum-dried at -50°C, 0.4 Pa to prepare bacterial powder. The bacterial concentration in the powder was determined by the dilution plate method, and the concentration should be between 5×10 8 CFU / g.

[0039] (4) Add 6% bacterial powder to the papermaking pulp, ferment at 37°C for 48 hours, and then papermake to form a substrate.

[0040] (5) Add 4% bacterial powder to the tobacco extract, ferment at 37°C for 12 hours, and then concentrate to form a coating solution.

[0041] (6) According to the preparation process of reconstituted tobacco leaves, coating is carried out, and then drying is carried out at 80°C. Sterilization can be achieved during drying. The moisture content of the dried reconstituted tobacco leaves is 12%.

[0042] Lignin degradation rate

[0043] The lignin degradation rates of reconstituted tobacco leaves treated with different concentrations of Pseudomonas paraflavum ZY-03 powder are shown in Table 1 below, which are 23.34% and 21.36%, respectively, and the lignin degradation effect is obvious.

[0044] Table 1 Lignin degradation rate

[0045]

[0046] (2) Sensory quality evaluation

[0047] The processed reconstituted tobacco shreds were added to the formulated tobacco and, after balancing in a constant temperature and humidity chamber, professional judges evaluated the aroma quality (A), aroma volume (B), concentration (C), softness (D), aftertaste (E), off-flavor (F), and pungency (G). Each indicator was scored on a 9-point scale, and the total sensory score was the sum of the scores for each sub-item. The evaluation results are shown in Table 2:

[0048] Table 2 Sensory evaluation score table

[0049]

[0050] From the data in the table, it can be seen that when the processed reconstituted tobacco shreds are added to the formula tobacco, the aroma volume and quality are improved, and the softness, impurities and stimulation are significantly improved compared with the control sample. The total scores are increased by 1.03 and 1.24 points respectively, and the overall sensory quality is significantly improved.

[0051] Although the present invention has been disclosed above by way of embodiments, they are not intended to limit the present invention. Any person skilled in the art may make various choices and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention is defined by the claims and their equivalents.

Claims

1. A method for improving the quality of reconstituted tobacco, characterized in that: The following steps are involved: S1: A single colony of Pseudomonas paraxanthinosus ZY-03 with the accession number CGMCC NO. 24422 was inoculated into YPD liquid medium and cultured at 22-37°C and a shaking speed of 100-250 rpm for 12-72 hours to obtain seed solution. S2: Centrifuge the seed solution at 8000-12000 r / min at 4°C for 10-20 min, remove the supernatant, add sterile water to reconstitute, and prepare a strain concentration of (1-2) × 10 9 A bacterial suspension of 100 CFU / mL was added with 1-3% glycerol, 1-5% sorbitol, 1-3% sodium ascorbate, and 2%-5% glucose as a protective agent, pre-frozen at -80°C for 8-12 hours, and vacuum-dried at -50°C, 0.4-2 Pa, to prepare bacterial powder; S3: According to the reconstituted tobacco preparation process, tobacco stems, tobacco dust or tobacco leaf fragments are extracted with water, and then solid-liquid separation is performed. The solids are then beaten and mixed with wood pulp to form paper pulp. 2-10% bacterial powder is added to the paper pulp, and the paper pulp is fermented at 30-40°C for 12-48 hours before being papered to form a reconstituted tobacco base sheet. S4: According to the reconstituted tobacco preparation process, tobacco stems, tobacco dust or tobacco leaf fragments are subjected to water extraction and solid-liquid separation, and the liquid is called tobacco extract; 2-8% bacterial powder is added to the tobacco extract, and the extract is fermented at 30-40°C for 12-48 hours and then concentrated to form a coating liquid; S5: According to the reconstituted tobacco preparation process, the coating liquid is evenly coated on the reconstituted tobacco base sheet, and then dried at 80-120° C. After coating, the moisture content of the dried reconstituted tobacco is 10-13%.

2. The method for improving the quality of reconstituted tobacco according to claim 1, characterized in that: The YPD liquid culture medium is prepared by high-pressure sterilization at 121° C. for 15 minutes using 10 g / L yeast powder, 20 g / L peptone, and 20 g / L glucose.

3. The method for improving the quality of reconstituted tobacco according to claim 1, characterized in that: In S2, the bacterial concentration in the bacterial powder was determined to be (2-8)×10 8 CFU / g.

4. The method for improving the quality of reconstituted tobacco according to claim 1, characterized in that: S3 is to add 6% bacterial powder to papermaking pulp, ferment it at 37°C for 48 hours, and then make paper to form a base sheet.

5. The method for improving the quality of reconstituted tobacco according to claim 1, characterized in that: In S4, 4% bacterial powder was added to the tobacco extract, and the extract was fermented at 37° C. for 12 hours and then concentrated to form a coating solution.

6. The method for improving the quality of reconstituted tobacco according to claim 1, characterized in that: In S5, the drying temperature is 80° C. and the moisture content is 12%.

7. Reconstituted shredded tobacco prepared by the method for improving the quality of reconstituted tobacco leaves according to any one of claims 1 to 6.

8. Use of the reconstituted shredded tobacco according to claim 7 in improving tobacco quality.

9. The use according to claim 8, characterized in that The processed reconstituted tobacco is added to the formula tobacco. After processing, the aroma volume and quality are improved, the softness, impurities and stimulation are significantly improved, and the overall sensory quality is improved.

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