A conductive hydrogel mediated by amino-modified graphene quantum dots and its preparation method and application

Abstract

The present invention belongs to the technical field of tissue damage repair, and specifically relates to a conductive hydrogel mediated by aminated graphene quantum dots, and its preparation method and application. The conductive hydrogel mediated by aminated graphene quantum dots provided by the present invention comprises a hydrogel obtained by photocuring acrylamide monomers, and aminated graphene quantum dots dispersed in the hydrogel; the mass of the aminated graphene quantum dots accounts for ≤0.3% of the mass of the acrylamide monomers. When the conductive hydrogel provided by the present invention is combined with exogenous electrical stimulation, an external current is applied to the wound to simulate the endogenous current at the wound, guiding dermal fibroblasts (DFs) to migrate and proliferate toward the wound along the electrical gradient, stimulating tissue growth, and thus accelerating wound healing.

Description

Technical Field

[0001] The present invention belongs to the technical field of tissue damage repair, and in particular relates to an amino-modified graphene quantum dot-mediated conductive hydrogel, a preparation method and an application thereof. Background Art

[0002] Wound healing is a complex physiological process that includes hemostasis, inflammation, proliferation, and remodeling stages. Any disturbance in these processes can lead to chronic or even non-healing wounds. Due to the complexity of healing, the reconstruction of skin function remains a challenge. Current wound treatment methods mainly include dressings, instruments, drugs, surgical interventions, and biological treatments. Although these methods have good therapeutic effects, they are either expensive or have adverse reactions such as rejection, infection, and allergies. Therefore, actively exploring new treatment options for wound healing has important scientific significance and clinical translation value.

[0003] Graphene derivatives, particularly graphene quantum dots (GQDs), possess high biocompatibility and antioxidant properties, scavenging reactive oxygen species by eliminating electron density within their conjugated ring structures. Furthermore, their abundant anionic functional groups on their edges make GQDs less likely to induce immune responses than positively charged nanoparticles. Current research suggests that GQDs alone can exert immunomodulatory effects.

[0004] However, the present invention has found in research that the repair gel prepared using graphene quantum dots has limited therapeutic effect on wound healing and has low clinical value. Summary of the Invention

[0005] The purpose of the present invention is to provide an amino-modified graphene quantum dot-mediated conductive hydrogel, and its preparation method and application. The amino-modified graphene quantum dot-mediated conductive hydrogel provided by the present invention has a better function of promoting wound repair when combined with exogenous electrical stimulation.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides an aminated graphene quantum dot-mediated conductive hydrogel, comprising a hydrogel obtained by photocuring an acrylamide monomer, and aminated graphene quantum dots dispersed in the hydrogel; the mass of the aminated graphene quantum dots accounts for ≤0.3% of the mass of the acrylamide monomer.

[0008] Preferably, the mass of the amino-modified graphene quantum dots accounts for 0.1 to 0.3% of the mass of the acrylamide monomer.

[0009] Preferably, the acrylamide monomer includes methacrylated gelatin.

[0010] The present invention provides a method for preparing a conductive hydrogel mediated by amino-modified graphene quantum dots according to the above technical solution, comprising the following steps:

[0011] Mixing acrylamide monomers, a photoinitiator, amino-modified graphene quantum dots and a solvent to obtain a mixed solution; the solvent includes water;

[0012] The mixed solution is photocured under ultraviolet light irradiation to obtain the conductive hydrogel mediated by the amino-modified graphene quantum dots.

[0013] Preferably, the mixing comprises the following steps:

[0014] dissolving an acrylamide monomer and a photoinitiator in water to obtain an acrylamide monomer solution;

[0015] dispersing the amino-polymerized graphene quantum dots in a buffer solution to obtain an amino-polymerized graphene quantum dot dispersion;

[0016] The acrylamide monomer solution and the amino-modified graphene quantum dot dispersion are mixed.

[0017] Preferably, the photoinitiator includes phenyl (2,4,6-trimethylbenzoyl) lithium phosphate; and the mass ratio of the photoinitiator to the acrylamide monomer is ≥0.025.

[0018] Preferably, the mass concentration of the acrylamide monomer in the acrylamide monomer solution is 0.05 to 0.2 g / mL.

[0019] Preferably, the mass concentration of the aminated graphene quantum dots in the aminated graphene quantum dot dispersion is 50 to 150 μg / μL.

[0020] The present invention provides the use of the conductive hydrogel mediated by aminated graphene quantum dots described in the above technical solution or the conductive hydrogel mediated by aminated graphene quantum dots prepared by the preparation method described in the above technical solution in the preparation of wound repair materials.

[0021] The present invention provides a wound repair dressing, comprising the conductive hydrogel mediated by the amino-dynated graphene quantum dots described in the above technical solution or the conductive hydrogel mediated by the amino-dynated graphene quantum dots prepared by the preparation method described in the above technical solution.

[0022] The present invention provides a conductive hydrogel mediated by amino graphene quantum dots, comprising a hydrogel obtained by photocuring acrylamide monomers, and amino graphene quantum dots dispersed in the hydrogel; the mass of the amino graphene quantum dots accounts for a percentage of the mass of the acrylamide monomers ≤ 0.3%. The present invention disperses amino graphene quantum dots (NH2-GQDs) in the hydrogel, and NH2-GQDs have the functions of conducting electricity, regulating oxidative stress and immunomodulating, and also have good antibacterial function; when the conductive hydrogel provided by the present invention is combined with exogenous electrical stimulation, an external current is applied to the wound to simulate the endogenous current at the wound, guiding dermal fibroblasts (DFs) to migrate and proliferate along the electrical gradient toward the wound, stimulating tissue growth, and thus accelerating wound healing. The results of the examples show that the conductive hydrogel mediated by amino graphene quantum dots provided by the present invention can further improve the proliferation and migration ability of DFs and better accelerate the healing of skin wounds in rats with dermal defects, and has good clinical application value.

[0023] Furthermore, in the present invention, the acrylamide monomer includes methacrylated gelatin (GelMA). The GelMA hydrogel is similar to extracellular matrix hydrogel (ECM), and has high biocompatibility, good release properties, and tissue adhesion. Therefore, the conductive hydrogel mediated by amino-modified graphene quantum dots provided by the present invention shortens wound healing time and improves healing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a comparison of the effects of the NH2-GQDs powder used in the embodiment of the present invention and the GQDs powder used in Comparative Example 1 on fibroblasts;

[0025] Figure 2 This is a comparison of the effects of the NH2-GQDs powder used in the embodiment of the present invention and the GQDs powder used in Comparative Example 1 on bacteria;

[0026] Figure 3 This is a comparison of the effects of the NH2-GQDs powder used in the embodiment of the present invention and the GQDs powder used in Comparative Example 1 on macrophages;

[0027] Figure 4 The UV spectrum and fluorescence spectrum of NH2-GQDs in the embodiment of the present invention are shown;

[0028] Figure 5 This is the fluorescence intensity spectrum of NH2-GQDs after dilution in an embodiment of the present invention;

[0029] Figure 6 This is the electron microscope morphology of NH2-GQDs in the embodiment of the present invention.

[0030] Figure 7 This is the Fourier infrared spectrum of NH2-GQDs according to an embodiment of the present invention;

[0031] Figure 8 The surface morphology of the conductive hydrogel mediated by NH2-GQDs synthesized in the embodiment of the present invention;

[0032] Figure 9 The electrochemical impedance spectroscopy of the conductive hydrogel mediated by NH2-GQDs synthesized in the embodiment of the present invention;

[0033] Figure 10 The electrochemical impedance spectroscopy of the conductive hydrogel mediated by NH2-GQDs synthesized in the embodiment of the present invention;

[0034] Figure 11 This is a flow chart of the repair effect of the conductive hydrogel mediated by NH2-GQDs synthesized in an embodiment of the present invention combined with exogenous electrical stimulation in a wound model;

[0035] Figure 12 This is a diagram showing the effect of voltage treatment on the migration ability of NH2-GQDs synthesized in an embodiment of the present invention combined with DFs of different sizes;

[0036] Figure 13 This is a bar graph showing the effect of voltage treatment on the migration ability of NH2-GQDs synthesized in an embodiment of the present invention in combination with DFs of different sizes;

[0037] Figure 14 This is a diagram showing the effect of voltage treatment on the proliferation ability of NH2-GQDs synthesized in an embodiment of the present invention combined with DFs of different sizes;

[0038] Figure 15 This is a diagram showing the healing of wounds treated with NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation synthesized in an embodiment of the present invention;

[0039] Figure 16 This is a graph showing the healing of wounds treated with NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation, as synthesized in an embodiment of the present invention;

[0040] Figure 17 This is a diagram showing the results of wound skin tissue reconstruction using NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation in an embodiment of the present invention. DETAILED DESCRIPTION

[0041] The present invention provides an aminated graphene quantum dot-mediated conductive hydrogel, comprising a hydrogel obtained by photocuring an acrylamide monomer, and aminated graphene quantum dots dispersed in the hydrogel; the mass of the aminated graphene quantum dots accounts for ≤0.3% of the mass of the acrylamide monomer.

[0042] In the present invention, unless otherwise specified, all preparation raw materials / components are commercially available products well known to those skilled in the art.

[0043] The conductive hydrogel mediated by amino-modified graphene quantum dots provided by the present invention includes a hydrogel obtained by photocuring acrylamide monomers.

[0044] In the present invention, the acrylamide monomer preferably includes methacrylated gelatin.

[0045] The conductive hydrogel mediated by aminated graphene quantum dots provided by the present invention comprises aminated graphene quantum dots dispersed in the hydrogel.

[0046] The present invention has no special requirements on the source of the amino-polymerized graphene quantum dots, and commercially available products can be used. In the present invention, the amino-polymerized graphene quantum dots are preferably purchased from Jiangsu Xianfeng Nanomaterial Technology Co., Ltd., with a product number of 102305 and a serial number of XF229.

[0047] In the present invention, the amino-modified graphene quantum dots are evenly distributed in the hydrogel, forming a uniformly distributed conductive network in the hydrogel, which enables the amino-modified graphene quantum dot-mediated conductive hydrogel to re-establish the original epidermal battery under exogenous electrical stimulation, simulate the endogenous current at the wound, shorten the healing time, and improve the healing efficiency.

[0048] In the present invention, the percentage of the mass of the aminated graphene quantum dots to the mass of the acrylamide monomer is ≤0.3%, preferably 0.1-0.3%, and more preferably 0.3%.

[0049] In the present invention, the percentage of the mass of the amino-polymerized graphene quantum dots to the mass of the acrylamide monomers should be neither too large nor too small. If the percentage of the amino-polymerized graphene quantum dots is too large, they will be toxic to cells to a certain extent, which may affect the wound healing effect. If the percentage of the amino-polymerized graphene quantum dots is too small, the conductivity of the conductive gel will be reduced, which may affect the therapeutic effect of the conductive gel on the wound.

[0050] The conductive hydrogel mediated by amino-modified graphene quantum dots provided by the present invention further comprises a photoinitiator and a solvent required for the photocuring of the acrylamide monomer. In the present invention, the solvent preferably comprises water.

[0051] The present invention provides a method for preparing a conductive hydrogel mediated by amino-modified graphene quantum dots according to the above technical solution, comprising the following steps:

[0052] Mixing acrylamide monomers, a photoinitiator, amino-modified graphene quantum dots and a solvent to obtain a mixed solution; the solvent includes water;

[0053] The mixed solution is photocured under ultraviolet light irradiation to obtain the conductive hydrogel mediated by the amino-modified graphene quantum dots.

[0054] The present invention mixes acrylamide monomers, a photoinitiator, amino-modified graphene quantum dots and a solvent to obtain a mixed solution; the solvent includes water.

[0055] In the present invention, the photoinitiator preferably includes lithium phenyl (2,4,6-trimethylbenzoyl) phosphate (LAP).

[0056] In the present invention, the mass ratio of the photoinitiator to the acrylamide monomer is preferably ≥0.025, more preferably 0.025.

[0057] In the present invention, the mixing preferably comprises the following steps:

[0058] dissolving an acrylamide monomer and a photoinitiator in water to obtain an acrylamide monomer solution;

[0059] dispersing the amino-polymerized graphene quantum dots in a buffer solution to obtain an amino-polymerized graphene quantum dot dispersion;

[0060] The acrylamide monomer solution and the amino-modified graphene quantum dot dispersion are mixed.

[0061] The present invention dissolves acrylamide monomer and photoinitiator in water to obtain an acrylamide monomer solution. In the present invention, the dissolution preferably includes: dissolving the photoinitiator in water to obtain a photoinitiator solution; mixing the acrylamide monomer and the photoinitiator solution under light-proof and heating conditions. In the present invention, the temperature for mixing the acrylamide monomer and the photoinitiator solution is preferably 60-70°C, and the holding time is preferably 30 minutes. In the present invention, the mass volume percentage of the photoinitiator solution is preferably 0.25% (w / v). In the present invention, in the acrylamide monomer solution, the mass concentration of the acrylamide monomer in the acrylamide monomer solution is 0.05-0.2g / mL, more preferably 0.1g / mL.

[0062] The present invention disperses the aminated graphene quantum dots in a buffer solution to obtain an aminated graphene quantum dot dispersion. In the present invention, the buffer solution is preferably a phosphate buffer solution (PBS solution), and the pH value of the PBS solution is 7.2 to 7.4. In the present invention, the mass concentration of the aminated graphene quantum dots in the aminated graphene quantum dot dispersion is preferably 50 to 150 μg / μL, more preferably 100 μg / μL.

[0063] After obtaining the acrylamide monomer solution and the aminated graphene quantum dot dispersion, the present invention mixes the acrylamide monomer solution and the aminated graphene quantum dot dispersion.

[0064] After obtaining the mixed solution, the present invention performs photocuring on the mixed solution under ultraviolet light irradiation to obtain the conductive hydrogel mediated by the amino-modified graphene quantum dots.

[0065] In the present invention, the ultraviolet light is preferably provided by an ultraviolet lamp. The light curing temperature is preferably room temperature, the curing time is preferably 30 seconds, and the wavelength of the ultraviolet light is preferably 365 nm.

[0066] The present invention provides the use of the conductive hydrogel mediated by aminated graphene quantum dots described in the above technical solution or the conductive hydrogel mediated by aminated graphene quantum dots prepared by the preparation method described in the above technical solution in the preparation of wound repair materials.

[0067] In the present invention, the conductive hydrogel mediated by amino-modified graphene quantum dots needs to be combined with exogenous electrical stimulation to achieve effective repair of the wound surface.

[0068] In the present invention, the external power source for the conductive hydrogel mediated by amino-modified graphene quantum dots is preferably a DC power source, and the voltage of the DC power source is preferably 0.1 to 3 V, more preferably 1 to 3 V, and more preferably 0.1 V, 0.5 V, 1 V, or 3 V. In the present invention, each power-on time is preferably 1 hour.

[0069] The present invention provides a wound repair dressing, comprising the conductive hydrogel mediated by the amino-dynated graphene quantum dots described in the above technical solution or the conductive hydrogel mediated by the amino-dynated graphene quantum dots prepared by the preparation method described in the above technical solution.

[0070] The wound repair dressing provided by the present invention needs to be combined with exogenous electrical stimulation to achieve effective repair of the wound surface.

[0071] The wound repair dressing provided herein requires the use of exogenous electrical stimulation to effectively repair the wound surface. In the present invention, the external power source for the wound repair dressing is preferably a DC power supply, with a voltage of preferably 0.1 to 3V, more preferably 1 to 3V; specifically preferably 0.1V, 0.5V, 1V, or 3V. In the present invention, each power-on period is preferably 1 hour.

[0072] The conductive hydrogel mediated by amino-modified graphene quantum dots provided by the present invention has the following characteristics:

[0073] (1) The GelMA hydrogel used in the present invention is similar to the extracellular matrix hydrogel (ECM), has high biocompatibility, good release properties, tissue adhesion, and has extremely high application value.

[0074] (2) The NH2-GQDs used in the present invention can regulate oxidative stress, have excellent electrical conductivity, immune regulation and good antibacterial function, and have broad application prospects.

[0075] (3) The present invention re-establishes the original epidermal battery through exogenous electrical stimulation, simulates the endogenous current at the wound, shortens the healing time, and improves the healing efficiency.

[0076] (4) The conductive hydrogel mediated by amino-modified graphene quantum dots provided by the present invention combined with exogenous electrical stimulation is expected to enhance the wound repair effect, provide a new solution for wound treatment, and provide support for clinical transformation.

[0077] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0078] Example 1

[0079] 300 μg of NH2-GQDs powder (Jiangsu Xianfeng Nanomaterial Technology Co., Ltd., product number 102305, serial number XF229, UV spectrum and fluorescence spectrum as shown in Figure 4 The fluorescence intensity spectra after dilution at different times are shown in Figure 5 The electron microscope morphology and Fourier infrared spectrum are shown in Figure 6 and 7 As shown, the NH2-GQDs were dispersed into 100 μg / μL NH2-GQDs dispersion using 3 μL PBS.

[0080] A LAP initiator aqueous solution with a mass volume concentration of 0.25% (w / v) was prepared, and then 0.1g of Gelma was added to 1mL of the LAP initiator aqueous solution, and heated to 60°C under light-proof conditions to prepare a Gelma solution with a mass volume concentration of 10% (w / v). A dispersion of NH2-GQDs with a concentration of 100μg / μL was added to the Gelma solution to make the concentration of NH2-GQDs 300μg / mL, and then cured with UV lamp for 30s to form an NH2-GQDs-mediated conductive hydrogel.

[0081] Figure 8 The surface morphology of the conductive hydrogel mediated by NH2-GQDs synthesized in the embodiment of the present invention;

[0082] Figure 9 and Figure 10 This is the electrochemical impedance spectroscopy of the conductive hydrogel mediated by NH2-GQDs synthesized in an embodiment of the present invention.

[0083] Comparative Example 1

[0084] 300 μg of GQDs powder was dispersed in 3 μL of PBS to prepare a 100 μg / μL GQDs dispersion.

[0085] A LAP initiator aqueous solution with a mass volume concentration of 0.25% (w / v) was prepared, and then 0.1g of Gelma was added to 1mL of the LAP initiator aqueous solution, and heated to 60°C under light-proof conditions to prepare a Gelma solution with a mass volume concentration of 10% (w / v). A GQDs dispersion with a concentration of 100μg / μL was added to the Gelma solution to make the concentration of GQDs 300μg / mL, and then cured with UV lamp for 30s to form a GQDs-mediated conductive hydrogel.

[0086] Test Example 1

[0087] Figures 1 to 3 The effects of the NH2-GQDs powder used in Example 1 of the present invention and the GQDs powder used in Comparative Example 1 on cells are compared. By comparing the effects of these two types of graphene quantum dots on fibroblasts, macrophages and bacteria, the present invention finds that compared with graphene quantum dots, the amino modification of amino-modified graphene quantum dots can better promote the migration of fibroblasts, promote the transformation of M2 pro-inflammatory macrophages into M1 anti-inflammatory macrophages, and inhibit Staphylococcus aureus and Escherichia coli. Therefore, the healing effect of amino-modified graphene quantum dots is better.

[0088] The NH2-GQDs-mediated conductive hydrogel prepared in Test Example 1 combined with exogenous electrical stimulation can better improve the migration and proliferation ability of DFs.

[0089] The main materials and sources used in Test Example 1 are as follows:

[0090] High-pressure sterilizer (Sanyo, Japan), biological safety cabinet (ESCO, Singapore), clean bench (Suzhou Purification Plant), cell horizontal centrifuge (Eppendorf, Germany), cell culture incubator (Thermo, USA), inverted biological microscope (Nikon, Japan), microplate reader (Thermofisher, USA), -80℃ refrigerator (Eppendorf, Germany);

[0091] Cell culture flasks (Fisher, USA), 6-well cell culture plates (Cellster, USA), 96-well cell culture plates (Cellster, USA), cell cryovials (Axygen, USA), 15 mL centrifuge tubes (JET Biofil, China), and CCK8 detection kit (Vazyme, China);

[0092] DFs cells: obtained by primary culture according to the method described in Chinese patent CN103881970A, entitled "Cultivation method of rat dermal mesenchymal stem cells".

[0093] according to Figure 11 The specific implementation steps of the flowchart and test example 1 are described as follows:

[0094] (1) Establishment of different DFs treatment groups: A total of 5 groups, namely Control group, NH2-GQDs group, NH2-GQDs / DC (0.1V) group, NH2-GQDs / DC (0.5V) group, and NH2-GQDs / DC (1V) group, were used for experiments.

[0095] (2) Determination of DFs cell migration ability: DFs were seeded in 6-well plates. After the cell confluence reached 100%, scratches were made on the cell layer using a pipette tip perpendicular to the cell plane. The cells were washed three times with sterile PBS to remove non-adherent cells, and then replaced with fresh serum-free medium. After placing the cells in a 37°C cell culture incubator, the cells were removed at 0 h, 12 h, and 24 h. The scratch width was measured under a microscope, and photos were taken for statistical analysis.

[0096] (3) Determination of DFs cell proliferation ability: DFs were seeded into 6-well plates. After the cells adhered to the wall, different treatments were added. After 48 hours, the cells were digested. Referring to the instructions of the CCK8 kit, DFs were seeded into four 96-well plates (2×10 3Each well was plated with 6 replicates. The medium in one 96-well plate was replaced daily with 100 μL of medium containing 10% CCK-8 reagent. A blank well without cells was also added with 100 μL of medium containing 10% CCK-8 reagent as a blank control. After incubation at 37°C for 3 hours, the absorbance at 450 nm was measured using a microplate reader. Higher absorbance values ​​indicate greater cell viability. Monitoring was continued for 4 consecutive days, and the data were statistically analyzed.

[0097] (4) Figure 12 and Figure 13 This is a diagram showing the effect of voltage treatment on the migration ability of NH2-GQDs synthesized in the embodiment of the present invention combined with DFs of different sizes. Figure 12 and Figure 13 It can be seen that the migration experiment results show that the co-treatment of NH2-GQDs with 1V DC voltage significantly improves the migration ability of DFs compared with the control group.

[0098] (5) Figure 14 This is a graph showing the effect of NH2-GQDs synthesized in the embodiment of the present invention on their proliferation ability after being combined with DFs of different sizes subjected to voltage treatment. Figure 14 It can be seen that the results of the proliferation experiment showed that co-treatment of NH2-GQDs with 1V DC voltage significantly promoted the proliferation of DFs compared with the control group.

[0099] Test Example 2

[0100] The NH2-GQDs-mediated conductive hydrogel prepared in Test Example 1 combined with exogenous electrical stimulation has a better effect in promoting wound repair.

[0101] The materials and sources used in Test Example 2 are as follows:

[0102] Male Sprague Dawley rats (SD rats) of about 180 g and 8 weeks old (Animal Center of Jiangsu University), ordinary rat chow and bedding (Animal Center of Jiangsu University), 10% chloral hydrate anesthetic (Tianjin Damao Chemical Reagent Factory), and 1 mL syringe (China Kangyou Company).

[0103] The specific implementation steps of Test Case 2 are described as follows:

[0104] (1) Establishment of rat wound model: 8-week-old male SD rats weighing about 180 g were shaved on the back, anesthetized by intraperitoneal injection of 10% chloral hydrate solution, and full-thickness cortical resection was performed on the back to create a circular wound with a diameter of 2 cm.

[0105] (2) Application of NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation therapy: The wound models were divided into 5 groups: GelMA group, GelMA / NH2GQDs group, GelMA / NH2GQDs / DC (0.5V) group, GelMA / NH2GQDs / DC (1V) group, and GelMA / NH2GQDs / DC (3V) group; each group had at least 4 animals. The gel was in situ solidified at the wound surface, and electricity was applied once every 2 days. The voltages used were 0.5V, 1V, and 3V, respectively, and the electricity was applied for 1 hour each time. The wound area was measured every 2 days, and photos were taken.

[0106] (3) Figure 15 This is a diagram showing the healing of wounds treated with NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation, synthesized in an embodiment of the present invention. Figure 16 This is a graph showing the healing of wounds treated with NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation synthesized in an embodiment of the present invention; Figure 15 and Figure 16 It can be seen that: from the 3rd day of wound repair, the GelMA / NH2GQDs / DC (0.5V) group, the GelMA / NH2GQDs / DC (1V) group, and the GelMA / NH2GQDs / DC (3V) group can significantly accelerate wound healing, and the GelMA / NH2GQDs / DC (3V) group heals faster; on the 13th day of wound repair, the GelMA group still has a larger wound, while the wound in the GelMA / NH2GQDs / DC (3V) group has almost completely healed, leaving only a tiny scab that is about to fall off.

[0107] (4) Figure 17 This figure shows the results of wound skin tissue remodeling using NH2-GQDs-mediated conductive hydrogel combined with exogenous electrical stimulation in an example of the present invention. Hematoxylin and eosin (HE)-stained skin tissue sections showed that compared with the control group, the GelMA / NH2GQDs / DC (3V) group significantly promoted wound epidermal regeneration, as evidenced by a clearer skin tissue structure.

[0108] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. Other embodiments can be obtained based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A conductive hydrogel mediated by amino-modified graphene quantum dots, characterized in that: The invention comprises a hydrogel obtained by photocuring an acrylamide monomer, wherein the acrylamide monomer is methacrylated gelatin; and amino graphene quantum dots dispersed in the hydrogel; the mass of the amino graphene quantum dots accounts for 0.1-0.3% of the mass of the acrylamide monomer.

2. The method for preparing the conductive hydrogel mediated by amino-modified graphene quantum dots according to claim 1, characterized in that: The following steps are involved: Mixing acrylamide monomers, a photoinitiator, amino-modified graphene quantum dots and a solvent to obtain a mixed solution; the solvent includes water; The mixed solution is photocured under ultraviolet light irradiation to obtain the conductive hydrogel mediated by the amino-modified graphene quantum dots.

3. The preparation method according to claim 2, characterized in that The mixing comprises the following steps: dissolving an acrylamide monomer and a photoinitiator in water to obtain an acrylamide monomer solution; dispersing the amino-polymerized graphene quantum dots in a buffer solution to obtain an amino-polymerized graphene quantum dot dispersion; The acrylamide monomer solution and the amino-modified graphene quantum dot dispersion are mixed.

4. The preparation method according to claim 2 or 3, characterized in that The photoinitiator includes phenyl (2,4,6-trimethylbenzoyl) lithium phosphate; the mass ratio of the photoinitiator to the acrylamide monomer is greater than or equal to 0.

025.

5. The preparation method according to claim 3, characterized in that The mass concentration of the acrylamide monomer in the acrylamide monomer solution is 0.05-0.2 g / mL.

6. The preparation method according to claim 3, characterized in that The mass concentration of the aminated graphene quantum dots in the aminated graphene quantum dot dispersion is 50-150 μg / μL.

7. Use of the conductive hydrogel mediated by amino-labeled graphene quantum dots according to claim 1 or the conductive hydrogel mediated by amino-labeled graphene quantum dots prepared by the preparation method according to any one of claims 2 to 6 in the preparation of wound repair materials.

8. A wound repair dressing, characterized in that: The conductive hydrogel comprises the conductive hydrogel mediated by the aminated graphene quantum dots according to claim 1 or the conductive hydrogel mediated by the aminated graphene quantum dots prepared by the preparation method according to any one of claims 2 to 6.

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