Humic acid wormwood compound fertilizer and preparation method thereof

Abstract

The present invention relates to the technical field of organic fertilizers, and specifically discloses a humic acid wormwood composite fertilizer and a preparation method thereof, which comprises wormwood extract A 1 2%, wormwood extract B 2 3%, soluble humic acid 3 5%, cellulose nanocrystals 0.3 0.5%, sodium alginate 0.1 0.2%, amino acid 0.3 0.5%, malic acid 0.01 0.03%, and the balance being deionized water, wherein the wormwood extract A is a substance extracted by steam distillation of wormwood; and the wormwood extract B is a substance extracted by protease water from the residue after wormwood extract A is extracted. The humic acid wormwood composite fertilizer of the present invention can not only effectively provide the required nutrients for crops, but also has the effect of insecticide and sterilization, can effectively prevent the impact of pests and diseases on crops, and improve the yield and quality of crops.

Description

Technical Field

[0001] The present application relates to the technical field of organic fertilizers, and more specifically, to a humic acid wormwood compound fertilizer and a preparation method thereof. Background Art

[0002] Organic liquid fertilizer is a complete, water-soluble fertilizer containing small-molecule organic matter, macronutrients, and trace elements. It boasts comprehensive nutrients, rapid fertilizer efficiency, and high utilization rate. Compared to traditional chemical fertilizers, it can be absorbed and utilized by plants in a shorter time, effectively preventing nutrient loss. Long-term application of organic fertilizer can effectively increase soil organic matter content, improve soil aggregate structure, reduce the disparity between organic and inorganic matter in the soil, increase the effective nutrient content in the soil, and promote crop growth and development. However, organic liquid fertilizers have the following problems: unstable nutrient content, prone to deterioration and decay, and reduced fertilizer efficiency. Mugwort, as a traditional Chinese herbal medicine, is also widely used in agricultural production, most commonly for controlling crop pests. Currently, mugwort essential oil is commonly used in agriculture. However, due to direct exposure to air, oxygen, temperature, light, and other factors, this oil is easily oxidized or degraded, resulting in poor stability and long-term storage and use. Furthermore, existing mugwort applications primarily utilize the leaves, with the overall utilization rate of the entire plant being low, resulting in significant waste. Summary of the Invention

[0003] The invention provides a humic acid mugwort compound fertilizer and a preparation method thereof, which solves the problems of unstable nutritional components and easy deterioration of existing organic liquid fertilizers, low mugwort utilization rate, and poor preservation of effective ingredients.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0005] A humic acid wormwood compound fertilizer comprises, by weight percentage, 1-2% wormwood extract A, 2-3% wormwood extract B, 3-5% soluble humic acid, 0.3-0.5% cellulose nanocrystals, 0.1-0.2% sodium alginate, 0.3-0.5% amino acids, 0.01-0.03% malic acid, and the balance being deionized water. The wormwood extract A is extracted from wormwood by steam distillation; and the wormwood extract B is extracted from the residue of the wormwood extract A by water extraction using protease.

[0006] Furthermore, preferably, the humic acid is fulvic acid.

[0007] Further, preferably: the humic acid wormwood compound fertilizer also includes atractylodes extract A and atractylodes extract B, the weight ratio of the atractylodes extract A to the wormwood extract A is 1:3; the weight ratio of the atractylodes extract B to the wormwood extract B is 1:5; the extraction method of the atractylodes extract A and the atractylodes extract B is the same as that of the wormwood extract A and the wormwood extract B.

[0008] Further, preferably: the specific extraction method of the wormwood extract A is as follows: the crushed wormwood and water are mixed in a volume ratio of 1:5-6, cellulase is added, the temperature is 50-60°C, the reaction is carried out for 1-2 hours, and then the microwave power is 200-300W, refluxed for 40-60 minutes, and the volatile oil is collected to obtain wormwood extract A.

[0009] Furthermore, preferably, the amount of cellulase added is 0.1% of the weight of wormwood, and the activity of the cellulase is 6000 U / g.

[0010] Furthermore, preferably: the specific extraction method of the mugwort extract B is as follows: the material after the mugwort extract A is extracted, then protease is added, the reaction is carried out for 30-50 minutes, and finally the filtrate is filtered to obtain the filtrate, the filtrate is filtered by a membrane, and vacuum dried to obtain the mugwort extract B.

[0011] Furthermore, preferably, the amount of protease added is 0.03% of the weight of wormwood, the protease is acidic protease, and the activity is 50,000 U / g.

[0012] Furthermore, preferably, the amino acids include a mixture of threonine, glutamic acid, glycine, methionine, phenylalanine, aspartic acid, proline, isoleucine, arginine and butyric acid.

[0013] The preparation method of the humic acid wormwood composite fertilizer of the present invention comprises the following steps:

[0014] (1) Mix the mugwort extract B, cellulose nanocrystals, sodium alginate, malic acid and deionized water evenly and set aside;

[0015] (2) adding the wormwood extract A dropwise to the solution of step (1) and emulsifying and dispersing for 1-2 minutes;

[0016] (3) adding the remaining raw materials to the solution of step (2), and subjecting the mixture to ultrasonic treatment for 10-20 minutes under ice bath conditions to obtain humic acid wormwood compound fertilizer.

[0017] Furthermore, preferably, the ultrasonic treatment has a power of 0.5-0.6 kW, an ultrasonic treatment time of 6 s, and a gap of 2 s.

[0018] Beneficial effects of the present invention:

[0019] The humic acid wormwood compound fertilizer of the present invention can not only effectively provide the required nutrients for crops, but also has the effect of killing insects and sterilizing insects, can effectively prevent the influence of diseases and pests on crops, and improve the yield and quality of crops.

[0020] The present invention adopts the whole plant of mugwort as the extract source, which can effectively improve the utilization rate of mugwort and promote the development of the mugwort industry.

[0021] The present invention uses humic acid, amino acids and mugwort extract as active ingredients, and the principle of mutual promotion and synergistic enhancement between them is as follows: (1) Humic acid can promote the absorption and transport of amino acids, amino acids can increase the stability of humic acid and nutrient release, and the active ingredients in mugwort extract can enhance the biological activity of humic acid and amino acids. This interaction can further improve the effect of liquid fertilizer; (2) Humic acid, amino acids and components in mugwort extract can be degraded and converted into forms that can be absorbed by plants by microorganisms in the soil. Humic acid and amino acids can serve as carbon and nitrogen sources for microorganisms, promote the reproduction and activity of beneficial microorganisms in the soil, and thus improve the release and conversion efficiency of nutrients. The active ingredients in mugwort extract can promote soil microbial activity and nutrient conversion; (3) Humic acid, amino acids and mugwort extract can increase the surface area and absorption capacity of plant roots, and promote the improvement of plant absorption and utilization efficiency of nutrients. Humic acid and amino acids can stimulate root growth and secretion of root substances, increase the contact area between roots and soil particles, and improve nutrient absorption. The active ingredients in mugwort extract can improve root physiological activity and enhance the plant's ability to absorb and utilize nutrients. Humic acid, amino acids, and mugwort extract work synergistically to enhance nutrient release and conversion, and improve plant nutrient absorption and utilization efficiency. This enhances the effectiveness of liquid fertilizers, promoting healthy plant growth and increasing yields.

[0022] The present invention uses cellulose nanocrystals, sodium alginate and mugwort extract B as auxiliary agents. Mugwort extract B is treated with cellulase and protease to convert the protein in mugwort into small molecular organic matter. In addition, mugwort extract B contains flavonoids, total phenolic acids and trace elements, which promote each other and have the following synergistic effects:

[0023] (1) Cellulose nanocrystals as carriers can provide structural support and adsorption capacity, increase the stability and bioactivity of sodium alginate and flavonoids, and sodium alginate and flavonoids can enhance the storage stability and nutrient release effect of cellulose nanocrystals. This interaction can further improve the effect of nutrients;

[0024] (2) The components of cellulose nanocrystals, sodium alginate, flavonoids and small molecular organic matter can be degraded and converted into forms that can be absorbed by plants by microorganisms in the soil. Cellulose nanocrystals can release organic matter and nutrients through microbial decomposition, thereby increasing soil fertility. Sodium alginate and flavonoids can interact with microorganisms in the soil to promote the release and conversion of nutrients. Small molecular organic matter has the characteristics of being easily absorbed and utilized by plant roots, which can improve the effectiveness and utilization of nutrients.

[0025] (3) Cellulose nanocrystals, sodium alginate, flavonoids and small molecular organic matter can increase the surface area and absorption capacity of plant roots, and promote the improvement of the efficiency of plant absorption and utilization of nutrients. Cellulose nanocrystals have a large specific surface area and porosity, which can provide more nutrients for plant roots. DETAILED DESCRIPTION

[0026] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0027] The raw materials used in the following examples of the present invention are as follows:

[0028] Extraction of mugwort extract A: Mugwort is selected from fresh mugwort produced in Nanyang that year, which is dried as a whole and then crushed into 60 mesh. The mugwort and water are mixed in a volume ratio of 1:6, and cellulase is added. The temperature is 60°C and the reaction is carried out for 1 hour. The amount of cellulase added is 0.1% of the weight of the mugwort, and the cellulase activity is 6000U / g. The extract is then added to a microwave extractor with a microwave power of 200W and refluxed for 60 minutes. The volatile oil is collected to obtain mugwort extract A.

[0029] The extraction of mugwort extract A by using cellulase plus microwave can not only effectively improve the extraction rate, but also effectively improve the extraction rate of subsequent mugwort extract B, thereby improving the utilization rate of mugwort.

[0030] Extraction of mugwort extract B: The specific extraction method of the mugwort extract B is as follows: the material after the mugwort extract A is extracted is then added with protease and reacted for 30 minutes. The amount of protease added is 0.03% of the weight of the mugwort, and the protease is an acidic protease with an activity of 50,000 U / g. Finally, the filtrate is filtered, the filtrate is filtered by a membrane, and vacuum dried to obtain mugwort extract B.

[0031] In this embodiment, the protease is used to treat the mugwort, which can effectively convert the protein in the mugwort into small molecular organic matter and increase the content of small organic molecules.

[0032] The extraction methods of Atractylodes lancea extract A and Atractylodes lancea extract B are the same as those of Artemisia argyi extract A and Artemisia argyi extract B, respectively, and will not be described here.

[0033] Amino acids include a mixture of threonine, glutamic acid, glycine, methionine, phenylalanine, aspartic acid, proline, isoleucine, arginine, and butyridine. This embodiment uses a combination of threonine, glutamic acid, glycine, aspartic acid, proline, and arginine in a weight ratio of threonine:glutamic acid:glycine:aspartic acid:proline:arginine = 2:2:1:2:2:1. The types and ratios of amino acids can be selected based on different plants, and complex amino acids can also be used.

[0034] Example 1

[0035] A humic acid wormwood compound fertilizer comprises, by weight percentage, 1% wormwood extract A, 2.5% wormwood extract B, 3% fulvic acid, 0.4% cellulose nanocrystals, 0.1% sodium alginate, 0.3% amino acid, 0.02% malic acid, and the balance being deionized water.

[0036] The preparation method thereof comprises the following steps:

[0037] (1) Mix the mugwort extract B, cellulose nanocrystals, sodium alginate, malic acid and deionized water evenly and set aside;

[0038] (2) adding the wormwood extract A dropwise to the solution of step (1), and using an emulsifying disperser for emulsification and dispersion at a speed of 3000 r / min for 1 min;

[0039] (3) The remaining raw materials are added to the solution of step (2), and ultrasonic treatment is performed for 10 minutes under ice bath conditions, with the ultrasonic treatment power being 0.6Kw, the ultrasonic treatment being performed for 6 seconds, and the interval being 2 seconds, to obtain humic acid wormwood composite fertilizer.

[0040] Example 2

[0041] A humic acid wormwood compound fertilizer comprises, by weight percentage, 2% wormwood extract A, 2% wormwood extract B, 4% fulvic acid, 0.3% cellulose nanocrystals, 0.2% sodium alginate, 0.4% amino acid, 0.01% malic acid, and the balance being deionized water.

[0042] The preparation method thereof comprises the following steps:

[0043] (1) Mix the mugwort extract B, cellulose nanocrystals, sodium alginate, malic acid and deionized water evenly and set aside;

[0044] (2) adding the wormwood extract A dropwise to the solution of step (1), and using an emulsifying disperser for emulsification and dispersion at a speed of 2000 r / min for 2 min;

[0045] (3) The remaining raw materials are added to the solution of step (2), and ultrasonic treatment is performed for 15 minutes under ice bath conditions, with the ultrasonic treatment power being 0.6Kw, the ultrasonic treatment being performed for 6 seconds, and the interval being 2 seconds, to obtain humic acid wormwood composite fertilizer.

[0046] Example 3

[0047] A humic acid wormwood compound fertilizer comprises, by weight percentage, 1.5% wormwood extract A, 3% wormwood extract B, 5% soluble humic acid, 0.5% cellulose nanocrystals, 0.15% sodium alginate, 0.5% amino acid, 0.03% malic acid, and the balance is deionized water. The wormwood extract A is obtained by composite extraction of wormwood using microwave and steam methods; and the wormwood extract B is obtained by composite water extraction of the residue after extraction of the wormwood extract A using protease and cellulase.

[0048] The preparation method thereof comprises the following steps:

[0049] (1) Mix the mugwort extract B, cellulose nanocrystals, sodium alginate, malic acid and deionized water evenly and set aside;

[0050] (2) adding the wormwood extract A dropwise to the solution of step (1), and using an emulsifying disperser for emulsification and dispersion at a speed of 3000 r / min for 1 min;

[0051] (3) The remaining raw materials are added to the solution of step (2), and ultrasonic treatment is performed for 20 minutes under ice bath conditions, with the ultrasonic treatment power being 0.5Kw, the ultrasonic treatment being performed for 6 seconds, and the interval being 2 seconds, to obtain humic acid wormwood composite fertilizer.

[0052] Example 4

[0053] The invention is basically the same as Example 1, except that: based on weight percentage, it includes 1% of mugwort extract A, 2% of mugwort extract B, 4.5% of soluble humic acid, 0.3% of cellulose nanocrystals, 0.1% of sodium alginate, 0.4% of amino acid, 0.02% of malic acid, and the balance is deionized water.

[0054] Example 5

[0055] It is basically the same as Example 1, except that: the humic acid wormwood compound fertilizer also includes atractylodes extract A and atractylodes extract B, and the weight ratio of atractylodes extract A to wormwood extract A is 1:3; the weight ratio of atractylodes extract B to wormwood extract B is 1:5. The specific ratio is as follows: a humic acid wormwood compound fertilizer, which includes, by weight percentage, wormwood extract A1%, wormwood extract B 2.5%, fulvic acid 3%, cellulose nanocrystals 0.4%, sodium alginate 0.1%, amino acid 0.3%, malic acid 0.02%, atractylodes extract A 0.33%, atractylodes extract B 0.5, and the balance is deionized water.

[0056] Comparative Example 1

[0057] The invention is basically the same as Example 1, except that: the wormwood extract A is not included, and the specific formula is as follows: a humic acid wormwood compound fertilizer, which includes, by weight percentage, 2.5% wormwood extract B, 3% fulvic acid, 0.4% cellulose nanocrystals, 0.1% sodium alginate, 0.3% amino acid, 0.02% malic acid, and the balance is deionized water. The preparation method comprises the following steps:

[0058] (1) Mix the mugwort extract B, cellulose nanocrystals, sodium alginate, malic acid and deionized water evenly and set aside;

[0059] (2) The remaining raw materials are added to the solution of step (1), and ultrasonic treatment is performed for 10 minutes under ice bath conditions, with the ultrasonic treatment power being 0.6Kw, the ultrasonic treatment being performed for 6 seconds, and the interval being 2 seconds, to obtain humic acid wormwood composite fertilizer.

[0060] Comparative Example 2

[0061] The invention is substantially the same as Example 1, except that the wormwood extract B is not included. The specific formulation is as follows: a humic acid wormwood compound fertilizer, comprising, by weight percentage, wormwood extract A1%, fulvic acid 3%, cellulose nanocrystals 0.4%, sodium alginate 0.1%, amino acid 0.3%, malic acid 0.02%, and the balance deionized water. The preparation method is similar to that of Example 1.

[0062] Comparative Example 3

[0063] The method is basically the same as Example 1, except that no protease is added during the extraction of the wormwood extract B.

[0064] Comparative Example 4

[0065] The invention is substantially the same as Example 1, except that cellulose nanocrystals are not included. The specific formulation is as follows: a humic acid wormwood compound fertilizer comprising, by weight percentage, 1% wormwood extract A, 2.5% wormwood extract B, 3% fulvic acid, 0.1% sodium alginate, 0.3% amino acid, 0.02% malic acid, and the balance being deionized water. The preparation method is similar to that of Example 1.

[0066] Comparative Example 5

[0067] Basically the same as Example 1, except that malic acid is not included, and the specific formula is as follows: a humic acid wormwood compound fertilizer, which includes, by weight percentage, wormwood extract A1%, wormwood extract B 2.5%, fulvic acid 3%, cellulose nanocrystals 0.4%, sodium alginate 0.1%, amino acid 0.3%, and the balance is deionized water.

[0068] Stability test:

[0069] At 0 h and 24 h, 0.1 ml of freshly prepared humic acid-wormwood compound fertilizer was added to 4.9 mL of a 0.1% (w / v) sodium lauryl sulfate solution. Vortex the mixture and measure the absorbance of the emulsion at 350 nm. The ESI was calculated as follows:

[0070] ESI=A0 / (A0-A 24 )*ΔT

[0071] In the formula, A0 represents the absorbance measured at 0h; A 24 The absorbance measured at 24 hours is shown in Table 1. ΔT represents the time difference (min). The experiment was repeated three times, and the average value was calculated. Data were processed using SPSS 25, and Duncan's test for significance was used. The results are shown in Table 1.

[0072] Table 1 ESI experimental data of humic acid wormwood compound fertilizer

[0073] ESI Example 1 44413.04±1270.76c Example 5 51863.16±1722.23b Comparative Example 1 70984.62±2460.19a Comparative Example 2 19883.48±382.80e Comparative Example 3 27253.20±300.86d Comparative Example 4 16875.98±179.89e Comparative Example 5 31515.40±402.36d

[0074] Note: Data are mean ± standard deviation. The same lowercase letters in the same column indicate no significant difference among the treatments with the same synergist, and different lowercase letters indicate significant difference (P < 0.05).

[0075] The mugwort extract A in the present invention is mugwort essential oil, which is processed by an emulsification encapsulation method. It can be seen that the mugwort extract B and cellulose nanocrystals have a significant impact on its stability. The active ingredients in the mugwort water extract B are adsorbed on the liquid contact surface to form a particle layer. These particles can cover droplets or micelles to prevent interaction between them. The presence of this physical barrier can prevent the fusion of droplets and the destruction of micelles, thereby maintaining the stability of the emulsion or latex system.

[0076] Sustained release effect:

[0077] Accurately weigh a certain weight of mugwort extract A and humic acid mugwort compound fertilizer, place them in a 60°C oven to evaporate for 12 hours, take them out and weigh them, calculate the loss rate of mugwort essential oil and emulsion, and repeat three times.

[0078] Loss rate of Artemisia argyi extract A (%) = (m 0A -m 12A ) / m 0A *100.

[0079] Loss rate of humic acid wormwood compound fertilizer (%) = (m 0肥料 -m 12肥料 ) / m 0肥料 *Content of Artemisia annua extract A*100

[0080] m 0A is the initial mass of Artemisia argyi extract A, m 12A The mass of Artemisia argyi extract A after drying for 12 hours;

[0081] m 0肥料 is the initial mass of humic acid wormwood compound fertilizer, m 12肥料 The fertilizer is the mass of humic acid mugwort compound fertilizer after drying for 12 hours.

[0082] The data were processed using SPSS 25 and the Duncan test was used to test the significance. The specific results are shown in Table 2.

[0083] Table 2 Volatility experimental data of humic acid wormwood compound fertilizer

[0084] average value Example 1 1.33±0.0067e Example 5 1.30±0.0058e Comparative Example 1 0.12±0.0036b Comparative Example 2 6.85±0.0088b Comparative Example 3 4.25±0.012c Comparative Example 4 7.98±0.020a Comparative Example 5 3.37±0.010d

[0085] Note: Data are mean ± standard deviation. The same lowercase letters in the same column indicate no significant difference among the treatments with the same synergist, and different lowercase letters indicate significant difference (P < 0.05).

[0086] As can be seen from the above table, the humic acid wormwood composite fertilizer of the present invention can effectively prevent the volatilization of essential oil by adding fiber nanocrystals and malic acid, improve its slow-release effect, and thus improve its efficacy.

[0087] Field trials:

[0088] The experiment took place at a planting base in Zhenping County, Nanyang City. The soil was sandy ginger black soil. Before planting, 20 kg of compound fertilizer and 2000 kg of decomposed pig manure were applied once per 667 m2. No topdressing was applied during the experiment. Cabbage was sown directly on August 20th using manual hole seeding. Row spacing was 50 cm × 70 cm, with ridge spacing of 60 cm. Each plot measured 2.6 m × 6.5 m, with 50 cabbages per plot. Cabbage variety: Improved Qingza No. 3.

[0089] The plots were randomly distributed with 80 cm isolation rows between each plot. No pesticides were applied. The humic acid and wormwood compound fertilizers of Example 1, Example 5, and Comparative Examples 1-5 were diluted 300-fold with water. A 25% chlorantraniliprole / indoxacarb suspension concentrate diluted 2000-fold and clean water were used as controls. Three replicates were set for each example. The water consumption per plot was 1.5 L.

[0090] Before application and 3, 7, and 14 days after application, 5 points were randomly marked in each plot, and 2 cabbages were surveyed at each point, for a total of 10 cabbages. The number of pests on the leaves of each cabbage plant was investigated and the number of live insects was counted.

[0091] Control effect = [1-(the number of insects in the blank control area before the drug application × the number of insects in the drug-treated area after the drug application) / (the number of insects in the blank control area after the drug application × the number of insects in the drug-treated area before the drug application)] × 100%.

[0092] When the cabbage was physiologically mature, 10 cabbages were randomly selected from each plot to measure the yield, and the number of plants in each plot was counted. Based on the plot area converted to 667m2, the yield was calculated to calculate the single plant weight, the number of surviving cabbages in the plot, and the yield.

[0093] The data were processed using SPSS25 and the Duncan test was used for significance. The specific results are shown in Tables 3 and 4.

[0094] Table 3 Experimental results of the control effect of different fertilizers

[0095] 3d(％) 7d(％) 14d(％) Example 1 45.38±0.03e 75.32±0.05c 95.73±0.37b Example 5 50.08±0.11d 82.19±0.20a 98.59±0.60a Comparative Example 1 25.13±0.02h 30.610±0.05g 50.98±0.13g Comparative Example 2 40.45±0.08g 70.63±0.05f 85.31±0.15f Comparative Example 3 43.31±0.06f 74.27±0.06d 93.18±0.07c Comparative Example 4 58.35±0.05b 73.26±0.03e 87.70±0.07h Comparative Example 5 55.38±0.02c 75.30±0.04c 90.07±0.14d Pesticide control 65.33±0.04a 78.46±0.10b 88.55±0.10e

[0096] As shown in the table above, the addition of mugwort extract to the present invention effectively prevents damage to Chinese cabbage by Plutella xylostella. Because mugwort extract is a botanical pesticide, its effect is slower than that of a 25% chlorantraniliprole / indoxacarb suspension concentrate. However, it exhibits a sustained-release effect, resulting in a long-lasting effect and a higher ultimate control efficacy than a 25% chlorantraniliprole / indoxacarb suspension concentrate. Example 5 also demonstrates that the present invention's combination of mugwort and atractylodes lancea effectively enhances insecticidal efficacy. Mugwort and atractylodes lancea have complementary antimicrobial effects. Mugwort leaves primarily contain volatile oils, flavonoids, and phenolic acids, exhibiting bactericidal, antibacterial, and antiviral properties; while atractylodes lancea contains volatile oils, flavonoids, and glycosides, exhibiting antibacterial, anti-inflammatory, and analgesic properties. The combination of the two enhances their insecticidal and antimicrobial effects, improving insecticidal and sterilization efficacy. The volatile oils in mugwort leaves and the flavonoids and glycosides in atractylodes lancea exhibit a synergistic effect. These ingredients work together to increase the permeability of bacterial cell membranes, disrupting the balance between the internal and external environments of bacteria, thereby increasing the sensitivity of pest bacteria to antimicrobial substances and achieving better insecticide and sterilization effects. The combined use of mugwort and turmeric can enhance their insecticide and antimicrobial abilities through complementary and synergistic effects.

[0097] Table 4 Experimental results of different fertilizers on single grain weight, number of surviving grains and yield per mu of white cabbage

[0098]

[0099]

[0100] As can be seen from the above table, the fertilizer of the present invention can effectively increase the single cabbage weight, survival rate and yield per mu of Chinese cabbage. Compared with the use of pesticides and water alone, the fertilizer of the present invention can effectively increase the single cabbage weight, survival rate and yield per mu. The yield per mu is increased by less than 25% compared with the use of pesticides.

[0101] Although the embodiments of the present invention have been described above, it will be apparent to those skilled in the art that modifications and substitutions made without departing from the principles and spirit of the present invention are intended to fall within the scope of protection claimed by the present invention.

Claims

1. A humic acid wormwood compound fertilizer, characterized by: Calculated by weight percentage, it includes 1-2% of mugwort extract A, 2-3% of mugwort extract B, 3-5% of soluble humic acid, 0.3-0.5% of cellulose nanocrystals, 0.1-0.2% of sodium alginate, 0.3-0.5% of amino acids, 0.01-0.03% of malic acid, and the balance is deionized water. The mugwort extract A is a substance extracted from mugwort using steam distillation; the mugwort extract B is a substance extracted from the residue after the mugwort extract A using protease water extraction.

2. The humic acid wormwood compound fertilizer according to claim 1, characterized in that: The humic acid is fulvic acid.

3. The humic acid wormwood compound fertilizer according to claim 1, characterized in that: The humic acid wormwood compound fertilizer also includes atractylodes extract A and atractylodes extract B, the weight ratio of atractylodes extract A to wormwood extract A is 1:3; the weight ratio of atractylodes extract B to wormwood extract B is 1:5; the extraction method of atractylodes extract A and atractylodes extract B is the same as that of wormwood extract A and wormwood extract B.

4. The humic acid wormwood compound fertilizer according to claim 1, characterized in that: The specific extraction method of the wormwood extract A is as follows: the crushed wormwood and water are mixed in a volume ratio of 1:5-6, cellulase is added, the temperature is 50-60°C, the reaction is carried out for 1-2 hours, and then microwave power is 200-300W, reflux for 40-60 minutes, and volatile oil is collected to obtain wormwood extract A.

5. The humic acid wormwood compound fertilizer according to claim 4, characterized in that: The added amount of the cellulase is 0.1% of the weight of the wormwood, and the activity of the cellulase is 6000 U / g.

6. The humic acid wormwood compound fertilizer according to claim 4, characterized in that: The specific extraction method of the wormwood extract B is as follows: the material after the wormwood extract A is extracted is then added with protease, reacted for 30-50 minutes, and finally filtered to obtain a filtrate, the filtrate is filtered using a membrane, and vacuum dried to obtain the wormwood extract B.

7. The humic acid wormwood compound fertilizer according to claim 6, characterized in that: The added amount of the protease is 0.03% of the weight of the wormwood; the protease is acidic protease with an activity of 50,000 U / g.

8. The humic acid wormwood compound fertilizer according to claim 1, characterized in that: The amino acids include a mixture of threonine, glutamic acid, glycine, methionine, phenylalanine, aspartic acid, proline, isoleucine, arginine and butyric acid.

9. A method for preparing the humic acid wormwood compound fertilizer according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) Mix wormwood extract B, cellulose nanocrystals, sodium alginate, malic acid and deionized water evenly and set aside; (2) Adding Artemisia argyi extract A dropwise to the solution of step (1) and emulsifying and dispersing for 1-2 minutes; (3) The remaining raw materials are added to the solution of step (2), and ultrasonic treatment is performed in an ice bath for 10-20 minutes to obtain humic acid wormwood compound fertilizer.

10. The method for preparing the humic acid wormwood compound fertilizer according to claim 9, characterized in that: The power of the ultrasonic treatment is 0.5-0.6Kw, the ultrasonic treatment is 6s, and the interval is 2s.

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