Alcoholism and hangover relieving traditional Chinese medicine composition, preparation method thereof, traditional Chinese medicine preparation, jelly drink and application

Abstract

The application discloses a traditional Chinese medicine composition for dispelling alcohol and its preparation method, a traditional Chinese medicine preparation, a jelly drink and application, and comprises the following substances: 15-60g of Chinese jujube seeds, 10-40g of green beans, 10-40g of sea buckthorn, 10-40g of dried tangerine or orange peel, 15-60g of mulberries, 5-10g of turmeric and 10-20g of hawthorn.The seven medicinal materials with the same source of food and medicine are combined, so that the traditional Chinese medicine composition for dispelling alcohol can promote the decomposition of ethanol in the body through various channels, reduces the damage of alcohol to the human body, especially to the liver, cardiovascular system, brain and blood vessels and the spleen and stomach, and has the health care effect on the liver, cardiovascular system, brain and blood vessels, and the spleen and stomach of the people who do not drink alcohol.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine and health food, more particularly, to a traditional Chinese medicine composition for dispelling and relieving alcoholism, a preparation method thereof, a traditional Chinese medicine preparation, a jelly drink and application. BACKGROUND

[0002] How to relieve and dispel alcoholism and reduce the damage of alcohol to human gastrointestinal, liver, cardiovascular and other organs is imminent, and it is of great significance and broad market prospect to develop effective alcoholism relieving and dispelling drugs or health food.

[0003] At present, the alcoholism relieving and dispelling products on the market are mainly chemical synthetic drugs and traditional Chinese medicine preparations. The chemical synthetic drugs can only promote the decomposition of alcohol and have a temporary relieving effect, and the chemical drugs will cause metabolic burden on the liver and kidney and have large side effects. The traditional Chinese medicine preparations have many traditional Chinese medicine ingredients and complex components, and some components of traditional Chinese medicine materials may have certain partiality or side effects. Moreover, these alcoholism relieving and dispelling drugs mainly focus on protecting liver function and ignore the damage of alcohol to other organs of the drinker, and cannot protect other organs while protecting the liver. SUMMARY

[0004] The present application aims to overcome the above-mentioned defects in the prior art and provide a traditional Chinese medicine composition for dispelling and relieving alcoholism, a preparation method thereof, a traditional Chinese medicine preparation, a jelly drink and application. The raw materials used are from the "List of Food and Medicine with Same Origin" issued by the National Health Commission of the People's Republic of China, which are safe and effective. The traditional Chinese medicine composition for dispelling and relieving alcoholism can promote the decomposition of ethanol in the body through multiple pathways and reduce the damage of alcohol to the human body, especially the liver, cardiovascular system and stomach and spleen.

[0005] To achieve the above-mentioned purpose, the technical scheme of the present application is as follows:

[0006] A traditional Chinese medicine composition for dispelling and relieving alcoholism comprises the following components in the following amounts: 15-60g of Hovenia dulcis Thunb, 10-40g of green beans, 10-40g of sea buckthorn, 10-40g of dried tangerine or orange peel, 15-60g of mulberry, 5-10g of turmeric and 10-20g of hawthorn.

[0007] The present application also discloses a preparation method of the traditional Chinese medicine composition for dispelling and relieving alcoholism as described above, comprising the following steps:

[0008] The green beans, sea buckthorn, dried tangerine or orange peel, mulberry, turmeric and hawthorn are decocted and extracted with water, and the extract is filtered and concentrated to obtain a decoction clear paste;

[0009] The Hovenia dulcis Thunb is extracted with ethanol by reflux, and the extract is filtered, ethanol is recovered and concentrated to obtain an alcohol extract clear paste;

[0010] Mix the decoction and the alcohol extract, to obtain the traditional Chinese medicine composition for dispelling alcohol and protecting the liver.

[0011] The application further discloses a traditional Chinese medicine preparation, which comprises the traditional Chinese medicine composition for dispelling alcohol and protecting the liver and pharmaceutically acceptable excipients.

[0012] The application further discloses a jelly drink, which comprises the traditional Chinese medicine composition for dispelling alcohol and protecting the liver and food acceptable excipients.

[0013] The application further discloses application of the traditional Chinese medicine preparation and the jelly drink in preparation of products for dispelling alcohol, protecting the liver and / or protecting the heart and blood vessels and / or invigorating the spleen and protecting the stomach.

[0014] The application has the following beneficial effects:

[0015] The application combines actual tests, and optimizes the specific composition and ratio of the traditional Chinese medicine composition for dispelling alcohol and protecting the liver, and uses seven traditional Chinese medicine materials with the same medicinal and edible functions to reasonably combine and compound, mainly comprising Ziziphus jujuba Mill, Mung bean and Curcuma longa L. with the functions of dispelling alcohol and protecting the liver, and further comprising Hippophae rhamnoides Linn, Citrus reticulata and Crataegus pinnatifida Bge. with the functions of invigorating the spleen and protecting the stomach and protecting the heart and blood vessels, so that the traditional Chinese medicine composition for dispelling alcohol and protecting the liver can promote decomposition of ethanol in the body through multiple channels, and reduce damage of alcohol to the human body, especially to the liver, heart and blood vessels, spleen and stomach. Animal experiment results show that the traditional Chinese medicine composition for dispelling alcohol and protecting the liver can increase the alcohol tolerance time of mice, and significantly shorten the drunken time of the mice, and has excellent effects of dispelling alcohol, protecting the liver, invigorating the spleen and protecting the stomach, and protecting the heart and blood vessels.

[0016] Therefore, the application better expands the application range of the composition by deeply understanding the functions and significance of the components in the composition, and has the simple preparation method, and effectively ensures that the effective components in the components can be fully extracted, so that the traditional Chinese medicine composition for dispelling alcohol and protecting the liver can not only effectively avoid toxic effects of acetaldehyde on the human body, prevent drunkenness and rapidly dispel alcohol, but also has the effects of protecting the heart and blood vessels, invigorating the spleen and protecting the stomach, and protecting the liver, realizes the strengthening of the two functions of dispelling alcohol and protecting the viscera, has the advantages of few medicinal materials, low cost, convenient taking, no obvious toxic and side effects, convenient daily carrying, timely taking of the people who dispel alcohol and protect the liver, and a wider market prospect. DETAILED DESCRIPTION

[0017] The present application is further described below in connection with specific embodiments, but is not limited in any way by the embodiments.

[0018] The present application firstly aims to develop a traditional Chinese medicine composition with excellent alcohol-eliminating and hangover-alleviating effects, and meanwhile investigates the maintenance of organs by the traditional Chinese medicine composition. In the development process, a large number of traditional Chinese medicine components are compounded, screened and verified, and it is accidentally found that, compared with other formulations, the combination of Hovenia dulcis Thunb, Mung bean, Hippophae rhamnoides Linn, Citrus reticulata, Morus alba Linn, Curcuma longa Linn and Crataegus pinnatifida Bge has not only good alcohol-eliminating and hangover-alleviating effects, but also plays a synergistic role in liver protection, cardiovascular and cerebrovascular protection and spleen and stomach protection.

[0019] The following are specific solutions:

[0020] The present application discloses a traditional Chinese medicine composition for alcohol-eliminating and hangover-alleviating, which comprises the following components in the following amounts: 15g-60g of Hovenia dulcis Thunb, 10g-40g of Mung bean, 10g-40g of Hippophae rhamnoides Linn, 10g-40g of Citrus reticulata, 15g-60g of Morus alba Linn, 5g-10g of Curcuma longa Linn and 10g-20g of Crataegus pinnatifida Bge.

[0021] Specifically, the traditional Chinese medicine composition for alcohol-eliminating and hangover-alleviating disclosed in the present application contains active ingredients beneficial to alcohol-eliminating and hangover-alleviating, and produces a synergistic effect after reasonable compounding, so that it can rapidly and effectively eliminate alcohol and alleviate hangover. Among them:

[0022] The Hovenia dulcis Thunb used in the present application has the effects of eliminating alcohol toxicity, stopping thirst and restlessness, stopping vomiting and benefiting urination and defecation; can accelerate ethanol metabolism, increase ADH activity, shorten hangover time and inhibit ethanol-induced muscle relaxation; and the flavonoid compounds contained therein have the effects of antioxidant, free radical scavenging, anti-inflammatory, antibacterial, antiviral and definite liver protection.

[0023] The Mung bean used in the present application has the effects of lowering blood lipids and cholesterol, resisting allergies, resisting bacteria, resisting tumors, enhancing appetite, protecting liver and kidney; is rich in protein, fat, carbohydrates and amino acids, can detoxify, and the flavonoid compounds are high-efficiency antioxidant components with strong antioxidant capacity.

[0024] The Hippophae rhamnoides Linn used in the present application has the effects of invigorating the spleen, digesting food, relieving cough and eliminating phlegm, and promoting blood circulation and dispelling fatigue; is used for treating spleen deficiency, poor appetite, food accumulation and abdominal pain, cough and excessive phlegm, chest pain and blood stasis; is rich in various vitamins, flavonoids, sterols, amino acids and trace elements and other active ingredients and nutrients, and has important nutritional value.

[0025] The Citrus reticulata used in the present application has the effects of regulating qi and invigorating the spleen, drying dampness and resolving phlegm; is used for treating abdominal distension, poor appetite, vomiting and diarrhea, and cough and excessive phlegm; is rich in flavonoid compounds, which are a large class of active ingredients of Rutaceae Citrus, promote the secretion of digestive juice, increase appetite, and have a certain degree of liver protection.

[0026] Mulberry used in the present application, the role of nourishing Yin and blood, and moistening dryness. Treatment of liver and kidney Yin deficiency, dizziness tinnitus, palpitations insomnia, premature gray hair, dry mouth thirst, internal heat polydipsia, dry constipation and other symptoms. Rich in glucose, sucrose, vitamins, etc., the nutritional value is very high. Mulberry contains a variety of functional ingredients, such as resveratrol, proanthocyanidins, rutin, etc., has the pharmacological effect of antioxidant, can enhance the activity of ethanol dehydrogenase in liver tissue, has the effect of accelerating ethanol metabolism to prevent drunk and dispel alcohol, but also has the effect of preventing and inhibiting the occurrence and development of cardiovascular and cerebrovascular diseases, reduce the risk of suffering from cardiovascular and cerebrovascular diseases.

[0027] Turmeric used in the present application, has the effects of promoting blood circulation to remove blood stasis, promoting qi and removing stagnation, and pungent dispersing and warming. It can dredge the meridians and treat chest and rib pain caused by liver qi stagnation and blood stasis. Curcumin can improve liver cells, reduce liver inflammatory substances, and increase liver detoxification and alcohol metabolism.

[0028] Hawthorn used in the present application, has the effects of promoting blood circulation and removing blood stasis, and converging liver qi and assisting the spleen and stomach. It can treat blood stasis caused by liver qi stagnation and has the effect of protecting the liver.

[0029] The present application preferably selects seven kinds of medicinal and edible homologous traditional Chinese medicines with the effects of dispelling alcohol and protecting organs, and combines them to make the traditional Chinese medicine composition for dispelling alcohol and protecting organs in the present application promote the decomposition of ethanol in the body through multiple pathways, and reduce the damage of alcohol to the human body, especially the liver, cardiovascular and cerebrovascular system, and the spleen and stomach.

[0030] The weight ratio of each component medicine in the traditional Chinese medicine composition for dispelling alcohol and protecting organs in the present application is further optimized and limited, the amount and effect of each component medicine are specifically adjusted and controlled, and the synergistic compatibility effect of each component medicine is better.

[0031] In a specific embodiment, the traditional Chinese medicine composition for dispelling alcohol and protecting organs includes the following amounts of Group : 15g of jujube, 10g of green bean, 10g of sea buckthorn, 10g of dried tangerine or orange peel, 15g of mulberry, 5g of turmeric, and 10g of hawthorn.

[0032] In a specific embodiment, the traditional Chinese medicine composition for dispelling alcohol and protecting organs includes the following amounts of Group : 30g of jujube, 20g of green bean, 20g of sea buckthorn, 20g of dried tangerine or orange peel, 30g of mulberry, 10g of turmeric, and 20g of hawthorn.

[0033] In a specific embodiment, the traditional Chinese medicine composition for dispelling alcohol and protecting organs includes the following amounts of Group : 60g of jujube, 40g of green bean, 20g of sea buckthorn, 20g of dried tangerine or orange peel, 30g of mulberry, 5g of turmeric, and 10g of hawthorn.

[0034] In addition, the present application also discloses a preparation method of the alcoholism relieving and hangover recovering traditional Chinese medicine composition according to any of the embodiments of the present application according to the characteristics of the specific functional components of each medicinal material in the alcoholism relieving and hangover recovering traditional Chinese medicine composition, and the preparation method comprises the following steps:

[0035] 1) decocting and extracting green beans, sea buckthorn, dried tangerine or orange peel, mulberry, turmeric and hawthorn with water, filtering and concentrating the extract to obtain a water decoction clear paste;

[0036] In a specific embodiment, step 1) specifically comprises:

[0037] 1.1) twice decocting and extracting sea buckthorn, dried tangerine or orange peel, mulberry, turmeric and hawthorn; the first time is decocting and extracting at a temperature of 100 DEG C for 2 hours, and the second time is decocting and extracting at a temperature of 100 DEG C for 1 hour, and the solid-liquid ratio of sea buckthorn, dried tangerine or orange peel, mulberry, turmeric and hawthorn during the extraction is 1: (8-10) (mass / volume ratio), filtering the extract after each decocting and extracting, and then combining the two filtrates and concentrating to obtain a clear paste;

[0038] 1.2) once decocting and extracting green beans by adding water and decocting at a temperature of 100 DEG C for 30 minutes, and the solid-liquid ratio of green beans during the decocting and extracting is 1:10 (mass / volume ratio), filtering the extract after the decocting and extracting, and then concentrating to obtain a clear paste;

[0039] 2) extracting jujube seeds by refluxing with ethanol, filtering the extract, recovering ethanol, and concentrating to obtain an ethanol extract clear paste;

[0040] In a specific embodiment, step 2) specifically comprises: twice refluxing jujube seeds; both times are refluxing at a temperature of 70 DEG C-80 DEG C for 2 hours, and the solid-liquid ratio of jujube seeds during the refluxing is 1: (8-10) (mass / volume ratio), filtering the extract after each refluxing, then combining the two filtrates, and concentrating to obtain an ethanol extract clear paste;

[0041] 3) mixing the clear pastes obtained in steps 1) to 2) to obtain the alcoholism relieving and hangover recovering traditional Chinese medicine composition.

[0042] The present application also discloses a traditional Chinese medicine preparation comprising the alcoholism relieving and hangover recovering traditional Chinese medicine composition and a pharmaceutically acceptable excipient; the alcoholism relieving and hangover recovering traditional Chinese medicine composition is the alcoholism relieving and hangover recovering traditional Chinese medicine composition according to any of the embodiments of the present application or prepared by the preparation method according to any of the embodiments of the present application.

[0043] In a specific embodiment, the pharmaceutically acceptable excipient is one or more of maltodextrin, sugar powder, dextrin, starch, sucrose, lactose, mannitol, xylitol, chitosan and bifidus.

[0044] In a specific embodiment, the mass ratio of the alcoholism-relieving traditional Chinese medicine composition and the malt dextrin is 1:(0.1-2).

[0045] In a specific embodiment, the dosage form of the traditional Chinese medicine preparation includes one or more than two of solid beverage, pill, powder, paste, tablet, granule and capsule. Preferably, the dosage form of the traditional Chinese medicine preparation is solid beverage.

[0046] The application also discloses a preparation method of the traditional Chinese medicine preparation according to any of the embodiments of the application, which comprises the following steps:

[0047] 1) mixing the alcoholism-relieving traditional Chinese medicine composition and the pharmaceutically acceptable excipient, adding 70%-85% ethanol by volume fraction to prepare soft material;

[0048] 2) sieving the soft material through a 20-mesh sieve to obtain wet granules;

[0049] 3) vacuum drying the wet granules at a temperature of 55-65 DEG C for 1 h, sieving the dried granules through a double-layer sieve with a 10-mesh upper layer and an 80-mesh lower layer and receiving the granules in a container, keeping the sieve horizontal, taking the middle-layer granules and vacuum packaging to obtain the traditional Chinese medicine preparation.

[0050] The application also discloses a jelly drink, which comprises the alcoholism-relieving traditional Chinese medicine composition and the food acceptable excipient; the alcoholism-relieving traditional Chinese medicine composition is the alcoholism-relieving traditional Chinese medicine composition according to any of the embodiments of the application or prepared by the preparation method according to any of the embodiments of the application.

[0051] In a specific embodiment, the food acceptable excipient is one or more than two of water, sugar powder, gelatin and potassium sorbate.

[0052] In a specific embodiment, the mass ratio of the alcoholism-relieving traditional Chinese medicine composition and the excipient is 1:3.

[0053] The application also discloses a preparation method of the jelly drink according to any of the embodiments of the application, which comprises the following steps:

[0054] mixing the alcoholism-relieving traditional Chinese medicine composition and the food acceptable excipient to pour into a mold to obtain the jelly drink.

[0055] Specifically, the preparation method described above adopts a preparation scheme of first extraction and then concentration for the seven medicinal materials in the formula, controls the extraction and concentration parameters, makes the effective components in the medicinal materials fully enter the extraction liquid, removes the non-effective components such as wood fiber in the medicinal materials, concentrates the extraction filtrate, and then adds the pharmaceutically acceptable excipient or the food acceptable excipient to prepare the instant traditional Chinese medicine granule or the jelly drink, which is beneficial to the absorption of the effective components and the full play of the drug efficacy, and is more convenient for storage, carrying and use.

[0056] The application also discloses application of the traditional Chinese medicine preparation or the jelly drink in preparation of a product for protecting liver and / or cardiovascular system and / or spleen and stomach. Specifically, the application expands the application range of the composition by deeply understanding the functions and significance of the components in the composition, so that the traditional Chinese medicine preparation and / or the jelly drink can not only prevent drunkenness, but also protect liver and cardiovascular system and spleen and stomach, and realize the intensification of the two functions of preventing drunkenness and protecting organs. In addition, the traditional Chinese medicine preparation and / or the jelly drink can be used for improving and regulating liver function and spleen and stomach function of non-drinking people.

[0057] The following is a specific embodiment

[0058] Embodiment 1

[0059] The traditional Chinese medicine composition for preventing drunkenness and sobering up in the embodiment comprises the following components in the following mass: 15g of Chinese jujube, 10g of mung bean, 10g of sea buckthorn, 10g of dried tangerine or orange peel, 15g of mulberry, 5g of turmeric and 10g of hawthorn.

[0060] The preparation method of the traditional Chinese medicine preparation in the embodiment comprises the following steps:

[0061] 1) sea buckthorn, dried tangerine or orange peel, mulberry, turmeric and hawthorn are extracted by twice water decocting; the first time is decocting for 2h under the condition that the temperature is 100℃, and the second time is decocting for 1h under the condition that the temperature is 100℃, the solid-liquid ratio of sea buckthorn, dried tangerine or orange peel, mulberry, turmeric and hawthorn is 1: (8-10) by mass / volume ratio during decocting, the filtrate obtained after each decocting is filtered respectively, and the filtrate obtained after the two times of filtering is combined and concentrated to obtain a clear extract;

[0062] 2) mung bean is extracted by once water decocting; the water is added and decocted for 30min under the condition that the temperature is 100℃, the solid-liquid ratio of mung bean is 1:10 by mass / volume ratio during decocting, the filtrate obtained after decocting is filtered and concentrated to obtain a clear extract;

[0063] 3) Chinese jujube is extracted by twice ethanol refluxing; both the two times of extraction are refluxed for 2h under the condition that the temperature is 65℃, the solid-liquid ratio of Chinese jujube is 1: (8-10) by mass / volume ratio during refluxing, the filtrate obtained after each refluxing is filtered respectively, and the filtrate obtained after the two times of filtering is combined and concentrated to obtain a clear extract;

[0064] 4) the water decocting clear extract and the alcohol extraction clear extract are mixed to obtain the traditional Chinese medicine composition for preventing drunkenness and sobering up;

[0065] 5) The alcoholism relieving and hangover recovering traditional Chinese medicine composition and malt dextrin are mixed according to the mass ratio of 1:1.5, and then 85% volume fraction of ethanol is added to prepare soft material;

[0066] 6) The soft material is granulated by passing through a 20 mesh sieve to obtain wet granules;

[0067] 7) The wet granules are vacuum dried at a temperature of 55-65°C for 1 hour, and then the dried granules are placed in a double-layer sieve with a top layer of 10 mesh and a bottom layer of 80 mesh and received in a container, and then sieved while maintaining a horizontal state, and the middle layer granules are taken and vacuum packaged to obtain the traditional Chinese medicine preparation.

[0068] Example 2

[0069] The alcoholism relieving and hangover recovering traditional Chinese medicine composition of this example includes the following components by mass: 30g of jujube fruit, 20g of mung bean, 20g of sea buckthorn, 20g of dried tangerine or orange peel, 30g of mulberry, 10g of turmeric, and 20g of hawthorn.

[0070] The preparation method of this example is the same as that of Example 1, and the traditional Chinese medicine preparation of this example is prepared according to the preparation method of Example 1.

[0071] Example 3

[0072] The alcoholism relieving and hangover recovering traditional Chinese medicine composition of this example includes the following components by mass: 60g of jujube fruit, 40g of mung bean, 20g of sea buckthorn, 20g of dried tangerine or orange peel, 30g of mulberry, 5g of turmeric, and 10g of hawthorn.

[0073] The preparation method of this example is the same as that of Example 1, and the traditional Chinese medicine preparation of this example is prepared according to the preparation method of Example 1.

[0074] Test Example

[0075] 1. Experimental materials

[0076] 1.1 Experimental animals

[0077] SPF level KM species mice, male, body weight 18.0-22.0g, provided by the animal center of Shenyang Pharmaceutical University, qualification certificate number SCXK(Liaoning)2020-0001, free access to food and water, and adaptively fed for three days before the experiment.

[0078] 1.2 Experimental medicinal materials and reagents

[0079] Hovenia acerba Lindl. of the Rhamnaceae family, the seed of Vigna radiata (Linn.) Wilczek of the Leguminosae family, the dried mature fruit of Hippophae rhamnoides L. of the Elaeagnaceae family, the dried mature pericarp of Citrus reticulata Blanco and its cultivated varieties of the Rutaceae family, the mature fruiting branch of Morus alba Linn. of the Moraceae family, the dried rhizome of Curcuma Longa L. of the Zingiberaceae family, and the dried mature fruit of Crataegus pinnatifida Bge. of the Rosaceae family.

[0080] Haiwangjintan tablets (Shenzhen Haiwang Health Co., Ltd.); Hongxing Erguotou liquor (56% vol, Beijing Hongxing Co., Ltd.).

[0081] 2. Experimental section

[0082] 2.1 Waking-up experiment

[0083] Fifty healthy male mice were randomly divided into five groups: a model group, a positive control group, an example 1 group, an example 2 group, and an example 3 group, with 10 mice in each group. After three days of adaptive feeding, the mice were fasted for 12 hours before the experiment. The model group was administered normal saline at a dose of 10 ml / kg, the positive control group was administered Haiwangjintan oral liquid at a dose of 10 ml / kg, and the groups of the present application were administered the mixed solution at a dose of 10 ml / kg according to the conversion of the doses of example 1, example 2, and example 3. Thirty minutes later, the mice were administered commercially available 56° Erguotou liquor at a dose of 15 ml / kg. The alcohol-induced sleep latency and the waking-up time of the mice were observed and recorded.

[0084] The alcohol-induced sleep latency was the time from the administration of Erguotou liquor to the complete disappearance of the righting reflex, and the waking-up time was the time from the complete disappearance of the righting reflex to the complete recovery of the righting reflex. The righting reflex was considered to have disappeared if a mouse remained in a supine position for more than 30 seconds, and vice versa.

[0085] Before the experiment, the mice were weighed and marked, and the administration dose and the liquor dose were set based on the pre-experiment.

[0086] 2.2 Effect of the present application on the serum ethanol concentration of mice

[0087] 60 healthy male mice were randomly divided into 6 groups: blank group, model group, positive control group, example 1 group, example 2 group, and example 3 group, 10 mice in each group. After adaptive feeding for three days, the mice were fasted for 12 hours before the experiment. The blank group and the model group were given 0.5% CMC-Na solution at a dose of 10 mL / kg by gavage, the positive control group was given Hydrangea solution (1 g / ml) at a dose of 10 mL / kg by gavage, and the example 1-3 groups were given mixed solutions (1.5, 3.0, and 3.7 g / ml of traditional Chinese medicine composition, respectively) at a dose of 10 mL / kg by gavage. After 30 minutes, the blank group was given normal saline by gavage, and the other groups were given Erguotou by gavage. Two hours after gavage, the mice were taken out of the cage, and the blood was taken from the eyeball and placed in a 1.5 mL centrifuge tube. The serum was separated and stored in a -80°C freezer for later use. The ethanol concentration in the serum was determined by gas chromatography, and the method was as follows:

[0088] a) Preparation of standard serum samples: 50 μL of blank serum was taken and divided into 7 centrifuge tubes (numbered 0, 1, 2, 3, 4, 5, and 6). Tube 0 was the blank (without ethanol) sample tube, and 10 μL of acetonitrile was added. Tubes 1-6 were standard sample tubes, and 10 μL of acetonitrile solution of a series of ethanol concentrations was added to each tube to prepare a series of standard serum samples with ethanol concentrations of 0.2, 0.5, 1, 2, 4, and 10 mg / mL, respectively.

[0089] b) Preparation of internal standard solution: 1 g of acetone was accurately weighed into a 100 mL volumetric flask, and acetonitrile was added to make up the volume to prepare an internal standard stock solution containing 10 mg / mL of acetone. 2 mL of the internal standard stock solution was accurately taken and placed in a 10 mL volumetric flask, and acetonitrile was added to make up the volume to prepare a 2 mg / mL internal standard solution.

[0090] c) Sample pretreatment: 10 μL of internal standard solution was added to each sample tube, and vortexed for 1 min. Then, 150 μL of acetonitrile was added, vortexed, and centrifuged at 12000 r·min-1 for 5 min. The supernatant was transferred to a clean centrifuge tube, diluted with 180 μL of acetonitrile, vortexed for 10 s, filtered with a filter membrane, and 1 μL was injected for analysis. -1

[0091] d) Gas chromatography conditions: Agilent DB-624 chromatographic column (30 m x 250 μm x 1.4 μm), injection port temperature 230°C, flame ionization detector (FID) temperature 250°C, programmed temperature, initial temperature 50°C, hold for 5 min, increase to 220°C at 60°C / min, hold for 1 min. Column flow rate 1 mL / min, split injection, split ratio 50:1, injection volume 1 μL.

[0092] ​e) Standard curve: take the standard serum sample, according to the "sample pretreatment" method, according to the "gas chromatography conditions" sample determination, record the peak area of ethanol and internal standard, take the ratio of the peak area of ethanol and internal standard as the ordinate, and take the ethanol concentration as the abscissa, draw the standard curve, and calculate the regression equation y = 2.0905x-0.0777 (r = 0.9988). The serum ethanol concentration has a good linear relationship in the range of 0.2 mg / mL-10 mg / mL.

[0093] f) Determination of serum samples: take the serum sample of the experimental mouse, add 10 μL of acetonitrile, according to the "sample pretreatment" method, according to the "gas chromatography conditions" sample determination, record the peak area of ethanol and internal standard, take the ratio of the peak area of ethanol and internal standard, calculate the concentration of ethanol in the serum sample by the standard curve method, and statistically process and compare the differences between groups.

[0094] 2.4 Statistical processing

[0095] SPSS21.0 statistical software is used for analysis and processing, and the data are represented by , and the comparison between groups uses one-way ANOVA, and P<0.05 indicates that the difference is significant, which has statistical significance.

[0096] 3, Experimental results

[0097] 3.1 The effect of the application on the drunkenness latency and sober-up time of mice, and the experimental results are shown in Table 1.

[0098] Table 1 Drunkenness latency and sober-up time of mice in each group n = 10

[0099] Group Latency / min Sobering time / min Model group 20.33±20.55 242.67±21.50 Positive control group 44.21±17.21 97.61±34.48** Example 1 46.33±9.23 94.150±43.16 Example 2 50.25±24.01 70.14±31.22** Example 3 53.20±25.41 63.28±29.17**

[0100] *: compared with the model group, * represents P<0.05, and ** represents P<0.01

[0101] From the results in Table 1, it can be seen that compared with the model group, the high, medium and low dose groups of the traditional Chinese medicine composition of the application can significantly shorten the drunkenness time of acute alcoholism mice (p<0.01), and can prolong the drunkenness latency of mice (p<0.01). In terms of prolonging the drunkenness latency and shortening the drunkenness time, the effects of the high, medium and low dose groups of the traditional Chinese medicine composition of the application are better than those of the Haiwang Jintan control group.

[0102] 3.2 The effect of the application on the ethanol concentration in the blood of mice, and the determination results of serum ethanol concentration are shown in Table 2.

[0103] Table 2 Serum ethanol concentration of mice n = 10

[0104] Group Serum ethanol content / mg / mL Blank group 0 Model group 7.39±0.49## Positive group 7.05±0.46 Example 1 5.17±0.82** Example 2 4.19±0.45** Example 3 3.69±0.56**

[0105] *: Compared with the blank group, # indicates P<0.05, ## indicates P<0.01; *: Compared with the model group, * indicates P<0.05, ** indicates P<0.01

[0106] After ethanol enters the human body, except for a small part being absorbed by the stomach, most of it is absorbed by the intestinal tract, and is completely absorbed into the blood within 30min-90min. The drunkenness performance has a direct relationship with the ethanol concentration in the blood, and the higher the ethanol concentration, the more serious the drunkenness. Therefore, investigating the ethanol concentration in the blood is an important index for evaluating the effect of alcohol elimination. The influence of the present application on the ethanol concentration in the serum of mice was understood by determining the high and low of the ethanol concentration in the serum of mice. It can be seen from the results in Table 2 that compared with the blank group, the ethanol concentration of the model group was significantly increased (P<0.01), indicating that the model was successfully replicated. Compared with the model group, in terms of reducing the ethanol concentration, the high, medium and low dose groups of the traditional Chinese medicine composition of the present application and the positive group can significantly reduce the ethanol concentration, and the difference has significant (P<0.01), and the effects of the high, medium and low dose groups of the traditional Chinese medicine composition of the present application are better than those of the Haowang Jindan control group.

[0107] 4, The weight ratio of the traditional Chinese medicine composition of the present application and the addition and subtraction formula research

[0108] The test example carries out weight ratio research and addition and subtraction formula research on the traditional Chinese medicine compound of the present application. The formula of the test drug is shown in the following table 3.

[0109] Table 3 Traditional Chinese medicine composition with different proportions (each medicinal material is expressed by mass)

[0110] Hovenia dulcis Thunb. Mung bean Hippophae rhamnoides L. Citrus reticulata Blanco Mulberry Curcuma longa L. Crataegus pinnatifida Bunge 1 15 10 10 10 15 5 10 2 30 20 20 20 30 10 20 3 60 40 20 20 30 5 10 4 10 10 10 10 15 10 20 5 15 10 10 10 10 5 10 6 15 10 10 10 0 10 10 7 15 0 10 10 15 10 10 8 15 10 10 10 15 0 10

[0111] Among them, group formula 1-3 is the prescription of embodiment 1-3; group formula 4 and group formula 5 adjust the weight ratio of each raw material in the compound; group formula 6 omits mulberry, group formula 7 omits mung bean, and group formula 8 omits turmeric, and the rest of the medicinal ingredients and the weight ratio are the same as group formula 1. Group formula 1-8 is prepared into a traditional Chinese medicine mixture according to the method of embodiment 1.

[0112] The experimental method is as follows: 100 healthy male mice are randomly divided into 10 groups: model group, positive control group, group formula 1-8, 10 in each group. After adaptive feeding for three days, the mice are fasted for 12h before the experiment, the model group is given physiological saline by gavage at a dose of 10ml / kg, the positive control group is given Haowang Jindan solution by gavage at a dose of 10ml / kg, and the present application group is given mixed solution by gavage at a dose of 10ml / kg according to group formula 1-8. 30min later, 15ml / kg of commercially available 56° Erguotou baijiu is given by gavage. The drunkenness latency and sober-up time of the mice are observed and recorded.

[0113] The mice were weighed and marked before the experiment, and the mice gavage dose and jar wine dose were set in combination with the pre-experiment, and the experimental results are shown in Table 4.

[0114] Table 4: Drunkenness latency and sober-up time of mice in each group n = 10

[0115] Group Latency / min Sobering time / min Model group 22.36±20.55 239.57±28.50 Positive control group 44.21±17.21 97.61±34.48** Group formula 1 46.33±9.23 94.15±43.16 Group formula 2 50.25±24.01 70.14±31.22** Group formula 3 53.20±25.41 63.28±29.17** Group formula 4 29.11±25.16 144.21±33.45 Group formula 5 44.17±22.14 99.21±35.27 Group formula 6 33.22±20.22 134.24±42.24 Group formula 7 27.39±25.41 156.55±43.12 Group formula 8 36.17±22.14 105.18±37.98

[0116] *: Compared with the model group, * represents P < 0.05, ** represents P < 0.01

[0117] As can be seen from the results in Table 3, after reducing some medicinal ingredients in the traditional Chinese medicine compound of the application, such as formula 6 without mulberry, formula 7 without mung bean and formula 8 without turmeric, although the drunkenness latency can be prolonged and the drunkenness time can be shortened to some extent (p < 0.05 compared with the model group), the effect is not as good as the original formula of the application, especially not as good as formula 1-3 in the original formula (all p < 0.01 compared with the model group). This shows that there may be a certain synergistic effect between the medicinal ingredients of the traditional Chinese medicine composition of the application. Especially under the weight ratio of formula 1-3, the traditional Chinese medicine composition of the application has the best effect in prolonging the drunkenness latency and shortening the drunkenness time, and has a very significant difference (p < 0.01) compared with the model group.

[0118] In summary, the specific composition and ratio of the traditional Chinese medicine composition for alcohol elimination and sobering up are preferred in combination with actual tests, and the seven medicinal materials with the same origin as food are combined and matched, so that the traditional Chinese medicine composition for alcohol elimination and sobering up of the application can promote the decomposition of ethanol in the body through multiple pathways, and reduce the damage of alcohol to the human body, especially the liver, cardiovascular system, brain and blood vessels, and spleen and stomach. The animal experiment results show that the traditional Chinese medicine composition for alcohol elimination and sobering up of the application can increase the tolerance time of mice to alcohol, and can significantly shorten the drunkenness time of experimental mice, and has excellent alcohol elimination effect on drunken mice, which shows that the traditional Chinese medicine composition for alcohol elimination and sobering up of the application not only can effectively avoid the toxic effect of acetaldehyde on the human body, achieve the prevention of drunkenness and rapid alcohol elimination and sobering up, but also has the effects of protecting cardiovascular system, brain and blood vessels, spleen and stomach, and liver, achieving the strengthening of the two functions of alcohol elimination and sobering up and protecting organs, and has the advantages of few medicinal ingredients, low cost, convenient taking, no obvious toxic and side effects, convenient daily carrying, suitable for timely taking by people with the need of alcohol elimination and sobering up, etc., and the market prospect is extremely broad.

[0119] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the scope of the application. It should be noted that for ordinary skilled persons in the art, without departing from the concept of the present application, several modifications and improvements can be made, which are all within the protection scope of the present application. Therefore, the protection scope of the present application patent should be subject to the appended claims.

Claims

1. A traditional Chinese medicine composition for alcoholism, characterized in that, The alcoholism relieving and hangover recovering traditional Chinese medicine composition is prepared from the following components in the following amounts: 15 g of Hovenia dulcis Thunb, 10 g of Mung bean, 10 g of Hippophae rhamnoides Linn, 10 g of Pericarpium Citri Reticulatae Viride, 15 g of Morus alba Linn, 5 g of Curcuma longa Linn, and 10 g of Crataegus pinnatifida Bge. The alcoholism relieving and hangover recovering traditional Chinese medicine composition is prepared from the following components in the following amounts: 30 g of Hovenia dulcis Thunb, 20 g of Mung bean, 20 g of Hippophae rhamnoides Linn, 20 g of Pericarpium Citri Reticulatae Viride, 30 g of Morus alba Linn, 10 g of Curcuma longa Linn, and 20 g of Crataegus pinnatifida Bge. The alcoholism relieving and hangover recovering traditional Chinese medicine composition is prepared from the following components in the following amounts: 60 g of Hovenia dulcis Thunb, 40 g of Mung bean, 20 g of Hippophae rhamnoides Linn, 20 g of Pericarpium Citri Reticulatae Viride, 30 g of Morus alba Linn, 5 g of Curcuma longa Linn, and 10 g of Crataegus pinnatifida Bge. The method for preparing the alcoholism relieving and hangover recovering traditional Chinese medicine composition comprises the following steps: 1) twice decocting and extracting Hippophae rhamnoides Linn, Pericarpium Citri Reticulatae Viride, Morus alba Linn, Curcuma longa Linn, and Crataegus pinnatifida Bge., the first time of decocting and extracting is carried out at a temperature of 100 ℃ for 2 h, and the second time of decocting and extracting is carried out at a temperature of 100 ℃ for 1 h, the solid-liquid ratio of Hippophae rhamnoides Linn, Pericarpium Citri Reticulatae Viride, Morus alba Linn, Curcuma longa Linn, and Crataegus pinnatifida Bge. during the extraction is 1: (8-10) by mass volume ratio, the extract after each time of decocting and extracting is filtered, and the two filtrates are combined and concentrated to obtain a clear extract; once decocting and extracting Mung bean, the decocting and extracting is carried out at a temperature of 100 ℃ for 30 min, the solid-liquid ratio of Mung bean during the decocting and extracting is 1:10 by mass volume ratio, and the extract after the decocting and extracting is filtered and concentrated to obtain a clear extract; 2) twice refluxing and extracting Hovenia dulcis Thunb, the refluxing and extracting is carried out at a temperature of 70-80 ℃ for 2 h, the solid-liquid ratio of Hovenia dulcis Thunb during the refluxing and extracting is 1: (8-10) by mass volume ratio, the extract after each time of refluxing and extracting is filtered, and the two filtrates are combined and concentrated to obtain an alcohol extract; mixing the clear extracts obtained in steps 1) and 2) to obtain the alcoholism relieving and hangover recovering traditional Chinese medicine composition.

2. A traditional Chinese medicine preparation for relieving alcoholism, characterized in that, The alcoholism relieving and hangover recovering traditional Chinese medicine composition is prepared from the following components in the following amounts: 15 g of Hovenia dulcis Thunb, 10 g of Mung bean, 10 g of Hippophae rhamnoides Linn, 10 g of Pericarpium Citri Reticulatae Viride, 15 g of Morus alba Linn, 5 g of Curcuma longa Linn, and 10 g of Crataegus pinnatifida Bge.

3. The hangover-relieving traditional Chinese medicine preparation according to claim 2, characterized in that, The pharmaceutically acceptable excipient is one or more of dextrin, starch, sucrose, lactose, mannitol, xylitol, chitosan, and bifidus; The mass ratio of the alcoholism relieving and hangover recovering traditional Chinese medicine composition to the excipient is 1: (0.1-2); The dosage form of the traditional Chinese medicine preparation is a solid beverage, a pill, a powder, a paste, a tablet, a granule, or a capsule.

4. The hangover-relieving traditional Chinese medicine preparation according to claim 2, characterized in that, The method for preparing the traditional Chinese medicine preparation comprises the following steps: mixing the alcoholism relieving and hangover recovering traditional Chinese medicine composition and the pharmaceutically acceptable excipient, adding 70-85% ethanol by volume fraction to obtain a soft material; granulating the soft material through a 20-mesh sieve to obtain wet granules; vacuum drying the wet granules at a temperature of 55-65 ℃ for 1 h, placing the dried granules in a double-layer sieve with the upper layer being a 10-mesh sieve and the lower layer being an 80-mesh sieve, and sieving the granules while keeping the container horizontal, taking the middle layer of the granules, and vacuum packaging the granules to obtain the traditional Chinese medicine preparation.

5. A jelly drink, characterized by, The alcoholism-relieving and liver-protecting composition is prepared from an alcoholism-relieving and liver-protecting traditional Chinese medicine composition and a food-acceptable excipient; the alcoholism-relieving and liver-protecting traditional Chinese medicine composition is as claimed in claim 1.

6. The jelly drink according to claim 5, characterized by The food-acceptable excipient is one or more of water, sugar powder, gelatin and potassium sorbate; The mass ratio of the alcoholism-relieving and liver-protecting traditional Chinese medicine composition to the excipient is 1:

3.

7. Use of the traditional Chinese medicine preparation as claimed in any one of claims 2-4 or the jelly drink as claimed in any one of claims 5-6 in the preparation of an alcoholism-relieving and liver-protecting product.