A composition for improving fish reproduction performance and its application

Abstract

The present invention relates to a composition for improving fish reproduction performance and a preparation method and application thereof, and belongs to the technical field of aquaculture. The composition for improving fish reproduction performance of the present invention includes the following components in weight parts per 1000 parts by weight of the composition: 25-150 parts of N-carbamylglutamic acid, 100-400 parts of motherwort, 50-300 parts of composite amino acids, 0.5-5 parts of composite vitamins, 10-100 parts of licorice extract and 10-100 parts of astragalus polysaccharides. The present invention selects N-carbamylglutamic acid, motherwort, composite amino acids, composite vitamins, licorice extract and astragalus polysaccharides to be a composition for improving fish reproduction performance, which has the advantages of improving fish reproduction performance, advancing its spawning time, increasing the number of eggs laid and the number of fertilized eggs and the hatching rate of fertilized eggs, and at the same time will not cause the advantage of increasing the deformity rate of fertilized eggs, and can significantly improve fish reproduction performance.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a composition for improving fish reproduction performance and application thereof. Background Art

[0002] Fish and other aquatic products are an important source of high-quality protein and an important part of my country's food security. Promoting the development of fish gonads and improving their reproductive performance has important economic value. However, there are few studies on the regulation of gonadal development of aquatic animals such as fish. The main methods to promote the development of fish gonads include: light, running water, temperature stimulation, nutritional supplementation, and sex hormone induction. However, the above traditional methods are generally applicable to gonad development after reaching VI to V, and the intervention is relatively delayed. The irregular use of sex hormones can easily lead to food safety hazards and have a single function. For this reason, it is urgent to find a method that can promote the early development of fish gonads and improve reproductive performance, help broodstock start gonadal development in advance, and improve their reproductive performance. Summary of the invention

[0003] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a composition and its application that can promote fish gonad development, enhance reproductive performance and promote fish spawning.

[0004] To achieve the above object, the technical solution adopted by the present invention is:

[0005] In a first aspect, the present invention provides a composition for improving the reproductive performance of fish, wherein every 1000 parts by weight of the composition comprises the following components in parts by weight: 25-150 parts of N-carbamylglutamate, 100-400 parts of motherwort, 50-300 parts of complex amino acids, 0.5-5 parts of complex vitamins, 10-100 parts of licorice extract and 10-100 parts of astragalus polysaccharide.

[0006] The present invention selects N-carbamylglutamate, motherwort, compound amino acids, compound vitamins, licorice extract and astragalus polysaccharide as a composition for improving fish reproduction performance, which has the advantages of improving fish reproduction performance, advancing the spawning time, increasing the number of eggs laid and the number of fertilized eggs and the hatching rate of fertilized eggs, and will not cause the fertilized egg deformity rate to increase, and can significantly improve fish reproduction performance. N-carbamylglutamate (N-Carbamylglutamate, NCG) is a stable analog of N-acetylglutamate (N-acetylglutamate, NAG), which can effectively activate the restriction enzyme in the arginine production pathway. The present invention reports for the first time that NCG is combined with motherwort compound amino acids, compound vitamins, licorice extract and astragalus polysaccharide as a composition, and it is verified through animal experiments that the composition containing NCG can significantly promote fish gonad development and improve fish reproduction performance, indicating that NCG has a certain effect of improving fish reproduction performance.

[0007] As a preferred embodiment of the composition for improving fish reproductive performance of the present invention, each 1000 parts by weight of the composition includes the following components by weight: 50-100 parts of N-carbamylglutamic acid, 150-300 parts of motherwort, 100-200 parts of complex amino acids, 1.5-2.5 parts of complex vitamins, 25-75 parts of licorice extract and 20-80 parts of astragalus polysaccharide. The inventors found in the experiment that the composition for promoting fish gonadal development has a relatively excellent effect of improving fish gonadal development and reproductive performance within a specific ratio range.

[0008] As a preferred embodiment of the composition for improving fish reproductive performance of the present invention, every 1000 parts by weight of the composition includes the following components by weight: 50 parts of N-carbamylglutamic acid, 200 parts of motherwort, 130 parts of complex amino acids, 2 parts of complex vitamins, 50 parts of licorice extract and 30 parts of astragalus polysaccharide. Under the preferred weight ratio, the prepared composition for improving fish reproductive performance has the best effect on improving fish gonad development and reproductive performance.

[0009] As a preferred embodiment of the composition for improving fish reproduction performance of the present invention, it includes at least one of the following (I) to (II):

[0010] (I) the composite amino acids include at least two of L-proline, L-serine, L-alanine, L-isoleucine, L-leucine, L-aspartic acid, L-tyrosine, L-glutamic acid, L-phenylalanine, L-arginine hydrochloride, L-lysine hydrochloride, L-valine, L-threonine, L-histidine hydrochloride, L-tryptophan, L-methionine, L-cystine and glycine;

[0011] (II) at least two of vitamin A, vitamin B1, vitamin B2, vitamin B4, vitamin B12, vitamin C, vitamin D, vitamin E, folic acid, pantothenic acid and biotin.

[0012] As a preferred embodiment of the composition for improving fish reproductive performance of the present invention, every 1 kg of the complex amino acids includes 2.4 g of L-proline, 2.4 g of L-serine, 4.8 g of L-alanine, 8.5 g of L-isoleucine, 11.8 g of L-leucine, 6.0 g of L-aspartic acid, 0.6 g of L-tyrosine, 1.8 g of L-glutamic acid, 12.8 g of L-phenylalanine, 12.0 g of L-arginine hydrochloride, 10.3 g of L-lysine hydrochloride, 8.6 g of L-valine, 6.0 g of L-threonine, 6.0 g of L-histidine hydrochloride, 2.2 g of L-tryptophan, 5.4 g of L-methionine, 0.2 g of L-cystine and 18.2 g of glycine.

[0013] As a preferred embodiment of the composition for improving fish reproductive performance of the present invention, each 1g of the complex vitamin includes 1.5mg of vitamin A, 3mg of vitamin B1, 3.4mg of vitamin B2, 3mg of vitamin B4, 0.009mg of vitamin B12, 90mg of vitamin C, 0.01mg of vitamin D, 30mg of vitamin E, 0.4mg of folic acid, 10mg of pantothenic acid and 0.03mg of biotin.

[0014] As a preferred embodiment of the composition for improving fish reproductive performance of the present invention, the composition for improving fish reproductive performance also includes starch supplemented to a total weight of 1000 parts.

[0015] In a second aspect, the present invention provides the use of the above composition for improving fish reproductive performance in the preparation of feed for improving fish reproductive performance.

[0016] In a third aspect, the present invention provides the use of the above-mentioned composition for improving fish reproductive performance in the preparation of feed for promoting fish spawning.

[0017] As a preferred embodiment of the application of the composition for improving fish reproductive performance of the present invention, the composition for improving fish reproductive performance accounts for 0.72-3.6% of the total weight of the feed for improving fish reproductive performance or the feed for promoting fish spawning.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention selects N-carbamylglutamate, motherwort, complex amino acids, complex vitamins, licorice extract and astragalus polysaccharide to form a composition for improving fish reproductive performance. The composition has the advantages of promoting fish gonad development, advancing the spawning time, increasing the number of eggs laid and the number of fertilized eggs and the hatching rate of fertilized eggs, and will not increase the deformity rate of fertilized eggs, thereby significantly improving fish reproductive performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is the ovarian section of the C4 control group in the effect example, the magnification is 20 times;

[0021] Figure 2 This is the ovarian section of the T4 control group in the effect example, the magnification is 20 times;

[0022] Figure 3 This is the ovarian section of the T3 control group in the effect example, the magnification is 20 times;

[0023] Figure 4 This is the ovarian section of the T5 control group in the effect example, the magnification is 20 times;

[0024] In the above figure, Eg2 is the oocyte in phase II, Eg3 is the oocyte in phase III, Eg4 is the oocyte in phase IV, and Eg5 is the oocyte in phase V. DETAILED DESCRIPTION

[0025] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0026] Unless otherwise specified, other materials, reagents, etc. used in the following embodiments and effect examples can be obtained from commercial sources.

[0027] In the following effect examples, the fish used is largemouth bass, which was provided by the experimental base of Pearl River Fisheries Research Institute.

[0028] In the following examples and comparative examples,

[0029] NCG, provided by Beijing Asia Pacific Xingmu Technology Co., Ltd., with a content of ≥97%.

[0030] The basic feed used in the experiment was produced by Zhaoqing Gaoyao Haida Biotechnology Co., Ltd. and the main nutrients were marked as follows:

[0031]

[0032] The complex amino acids and complex vitamins are provided by DSM (China) Co., Ltd., and each 1kg of the complex amino acids includes 2.4g L-proline, 2.4g L-serine, 4.8g L-alanine, 8.5g L-isoleucine, 11.8g L-leucine, 6.0g L-aspartic acid, 0.6g L-tyrosine, 1.8g L-glutamic acid, 12.8g L-phenylalanine, 12.0g L-arginine hydrochloride, 10.3g L-lysine hydrochloride, 8.6g L-valine, 6.0g L-threonine, 6.0g L-histidine hydrochloride, 2.2g L-tryptophan, 5.4g L-methionine, 0.2g L-cystine and 18.2g glycine; each 1g of the complex vitamins includes 1.5mg vitamin A, 3mg vitamin B1, 3.4mg vitamin B2, 3mg vitamin B4, 3mg vitamin B12. 0.009mg, vitamin C 90mg, vitamin D 0.01mg, vitamin E 30mg, folic acid 0.4mg, pantothenic acid 10mg and biotin 0.03mg.

[0033] Examples 1-5

[0034] Examples 1-5 respectively provide a composition for improving the reproductive performance of fish, and the components and dosages thereof are shown in Table 1. The preparation method of the composition for improving the reproductive performance of fish is to mix the components to obtain the composition.

[0035] Table 1 Components and dosages of different compositions for improving fish reproduction performance (by weight)

[0036]

[0037] Comparative Examples 1-4

[0038] Comparative Examples 1-4 respectively provide a composition for improving the reproductive performance of fish, the components and dosages of which are shown in Table 1, and the preparation method is the same as that of Example 1.

[0039] Effect Example 1

[0040] In order to verify the efficacy of the composition for improving fish reproductive performance of the present invention, the composition for improving fish reproductive performance of Example 1 was subjected to animal experiments, and the specific scheme is as follows:

[0041] 1. Experimental content

[0042] 1.1 Experimental grouping and preliminary treatment

[0043] The experiment started on August 20, 2021 and ended on October 29, 2021, for a total of 10 weeks. A total of 9 experimental groups were set up in this experiment, with 3 cages (repeated) in each group and 25 fish in each box. The experimental fish were randomly selected from self-cultivated cages with healthy physique and uniform size.

[0044] 7200 mg of the compositions obtained in Example 1, Example 2, Example 3, Example 4, Example 5 and Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4 were added to each 1 kg of the basic feed for largemouth bass, and the samples were named as Group T1, Group T2, Group T3, Group T4, Group T5 and Group C1, Group C2, Group C3, Group C4, respectively. The experimental breeding facility was an outdoor pond with cages, and the pond area was 2292 m 2 The average water depth is 2m and the cage specifications are: 200cm×200cm×80cm.

[0045] Before the experiment, ordinary feed was used for 7 days. The experiment was started after the fish adapted to the new environment and had normal feeding. The pond water quality was tested every 7 days. The basic requirements were: pH was stable at 7.5-9.0, dissolved oxygen (DO) was not less than 5 mg / L for 24 hours a day, total ammonia nitrogen ≤1.2 mg / L, nitrite ≤0.15 mg / L, water temperature (35±3℃), and the experiment was completely open-air with natural light. Feed was fed at 2% of the fish body weight at 08:00 and 16:30 every day. During the breeding process, 5 fish were randomly sampled from each group every 2 weeks to measure their weight, evaluate and determine the feeding rate, and if abnormal fish (swimming alone, emaciated, black body, ulcers on the body surface, etc.) were found, they were promptly removed, the cause of death was analyzed, and records were kept.

[0046] The fish fed with the composition for improving the reproductive performance of fish were used as the pre-natal intensive cultivation fish samples. The samples were collected as female ovaries and male testes. After the samples were fixed with 4% paraformaldehyde, they were strictly followed the SOP procedures for pathological laboratory examination: trimming → dehydration → embedding → slicing → staining → sealing → microscopic examination. Qualified slices were observed under a microscope, and the tissue slices were observed in detail at 20, 200, and 400 times. The gonad slices were observed, and the changes in the gonad development in the slices were recorded, and the gonad index GSI (GSI (%) = ovary or testis mass (g) / body mass (g)) was counted. Such as: the phase of gonad development and the composition of the cells. During and after the spawning process, the loss of broodstock in each pool was counted.

[0047] 1.2 Spawning and reproduction

[0048] Indoor spawning and breeding were adopted. From November 22, 2021 to December 16, 2021, a total of 25 days. The experimental fish were experimental fish that were intensively cultivated before pre-production. The fish in each group of the experiment were selected with an average size of 475.0±5.0g / tail or above. To ensure the safety of the experimental process, the fish used in the experiment were tested for viruses by fluorescent quantitative PCR again before the experiment. None of them carried LMBV, RANA, ISKNV, VNN and parasitic pathogens, and they were in good health. Each group has 15 fish, including 9 female fish and 6 male fish. The fish are grouped and numbered, which still corresponds to the previous T1, T2, T3, T4, T5 and C1, C2, C3, and C4 groups. Each group has 3 parallels and is placed in 27 indoor cement pools. The cement pool has a length: width: height ratio of 400cm×400cm×150cm. It is equipped with a Notz fan 1500W aerator. The inlet and outlet water use 110mm PVC water pipes, and the inlet and outlet water are independent. The aquaculture water uses underground deep well water, which is injected into the reservoir for standby after 24 hours of aeration. For daily feeding and feeding, continue to use the same feed corresponding to each group in the previous experiment. The pond water quality was tested regularly every day. The general requirements were as follows: pH was maintained at 7.5-8.0, dissolved oxygen (DO) was not less than 5 mg / L for 24 hours a day, total ammonia nitrogen ≤ 0.6 mg / L, nitrite ≤ 0.1 mg / L, water temperature (30 ± 3 ° C), and the test was completely indoor. The polyethylene (PE) black and white grid film blocked part of the light, followed the sunlight during the day, and no additional light was given at night. Feed was fed at 2% of the fish body weight at 08:00 and 16:30 every day. Artificial fish nests were set up after one week, the spawning situation was observed, and the artificial fish nest brown pieces with fertilized eggs were collected in time, and the corresponding data were recorded and counted.

[0049] Incubation of fertilized eggs. This experiment adopted artificial centralized oxygen incubation, and hatching pools were set up in corresponding groups. As before, T1, T2, T3, T4, T5 and C1, C2, C3, C4 groups, 3 parallels in each group, 27 indoor cement pools were placed, the cement pool specifications length: width: height ratio is: 200cm×200cm×100cm, the incubator specifications length: width: height ratio is: 80cm×40cm×60cm, equipped with a Notz fan 1500W aerator, 110mm PVC water pipes are used for inlet and outlet, and the inlet and outlet are independent. The aquaculture water is deep underground well water, which is injected into the reservoir for standby after 24 hours of aeration. The basic requirements are: pH value is stable at 7.5-8.0, dissolved oxygen (DO) ≥ 6.0mg / L, nitrite ≤ 0.1mg / L, total ammonia nitrogen ≤ 0.6mg / L, water temperature (25±3℃), the hatchery is completely indoor, polyethylene transparent film, follow the sun during the day, and no supplementary light at night. Observe the rupture of fertilized eggs at 08:00 and 16:30 every day, the waist point of juvenile fish, also known as "water splash", appears, and the number of juvenile fish is counted 24 hours after "surfacing" and the deformity of juvenile fish is observed.

[0050] At 8:00 a.m. every day, the spawning situation of each breeding pool was inspected manually, the fertilized eggs of each group were collected in time, and the artificial spawning nests of each group were counted. Two brown pieces of 3-day fertilized eggs were randomly selected from each group, and then the brown pieces of fertilized eggs of each group were debonded with 0.6% sterilized cooled saline plus 10 mg / kg talcum powder. 1 g of fertilized eggs were randomly selected and placed in a special white fry dish with an appropriate amount of clean water. After the fertilized eggs were naturally spread out, they were photographed with a macro camera and printed out, combined with browsing and observation on a computer, and then manually counted. After each group was repeated 3 times, all the fertilized eggs were counted and counted using an electronic gram scale.

[0051] The fertilized eggs after debonding treatment were placed in the hatching pool for oxygen incubation. 1g of fertilized eggs were randomly selected from each group. Three groups were selected from each experimental group and placed in the incubator for oxygen incubation. After the juveniles "came up to the water" and swam horizontally, they were placed in a special plate for white fry. An appropriate amount of clean water was added. After the "juveniles" spread out naturally, the pictures were taken with a camera at a macro distance and printed. Combined with computer browsing and observation, manual counting was performed. 1g of juveniles that "came up to the water" and swam horizontally were selected from each group of hatching pools. The water was filtered and placed in a special plate for white fry. An appropriate amount of clean water was added. After the "juveniles" spread out naturally, the pictures were taken with a camera at a macro distance and printed. Combined with computer browsing and observation, manual counting was performed. The total weight of juveniles in each group was counted using an electronic gram scale. Deformity rate statistics Randomly select 1g of juveniles and place them in a special plate for white fry. After preliminary judgment by naked eye observation, the statistics were confirmed using a microscope. Each pool was repeated 3 times. Those with spinal curvature, caudal peduncle folds, incomplete development, etc., which were significantly different from normal individuals, were counted as deformed fish.

[0052] 2. Experimental results

[0053] 2.1 Effect of the composition for improving fish reproductive performance on the gonadal development of largemouth bass

[0054] Before the breeding experiment began, each group randomly selected some individuals for dissection observation and calculation of gonad index. Qualified slices were observed under a microscope, and the gonad slices were read at 20, 200, and 400 times, and the gonad development status was marked in time, such as: the phase of gonad development, the composition of the cells, etc. HE staining, slice magnification 400 times and observation, gonads, testes, and ovaries were randomly selected for observation, and ovarian slices of different combination addition dose groups were 20 times HE stained, and the results are shown in the table. Figure 1-4 .

[0055] As shown in Table 2, the gonadal indexes of the experimental groups were better than those of the control group, and the gonadal indexes of the T2 group and the T3 group were 10.9% and 61.8% higher than those of the control group, respectively. With the increase in the amount of the composition added, the gonadal weights of the female and male in the T1, T2, and T3 groups were significantly greater than those of the C4 control group and the T4 and T5 groups (P<0.05).

[0056] Table 2 Effects of different concentrations of compositions for improving fish reproductive performance on gonadal development of largemouth bass

[0057]

[0058] like Figure 1 As shown in the figure, the ovaries of the C4 control group remained in phase II, and the ovaries still retained a large number of primary oocytes and phase II oocytes; phase III oocytes began to appear. Figure 2 As shown in the figure, the ovaries of the T4 group stayed in phase III and transitioned to phase IV. The ovaries had abundant oocytes in phase III and oocytes in phase IV began to appear. Figure 3 As shown in the figure, the ovaries of the T3 group remained in phase IV and transitioned to phase V. There were abundant phase IV oocytes in the ovaries, and the phase IV oocytes also differentiated in large numbers. Figure 4 As shown in the figure, the ovaries of the T5 group stayed in phase III and transitioned to phase IV. There were abundant oocytes in phase III and phase IV in the ovaries. Through slice observation, it was found that the ovarian development of the T3 and T4 groups was more mature than that of the C4 control group, while the ovarian development of the T5 group was not as good as that of the C4 control group. Therefore, it was speculated that excessive addition of the composition in the diet may delay ovarian development, and the data of later spawning and reproduction also verified this speculation. There was no significant difference in gonadal index between the experimental group and the control group (P>0.05), but there was a significant difference in the weight of the male fish testis between the T3, T4 and C4 control groups (P<0.05). The typical phenotype of HE slices showed that the secondary oocyte division development of the T3 and T4 supplemented groups was advanced, and the V and IV phase oocytes appeared earlier than the control group. In the later reproduction test, the spawning time of the T3 and T4 groups was advanced, and the number and quality of fertilized eggs were significantly better than those of the C4 control group.

[0059] 2.2 Composition for improving fish reproductive performance

[0060] As shown in Table 3, there was no significant difference in the loss of broodstock during the breeding process of each experimental group (P>0.05), but the survival rate of each experimental group was higher than that of the C4 control group, among which the survival rate of T2 and T3 groups was higher. The autopsy found that the liver was ruddy, the spleen had no abnormal swelling, the intestinal abdominal fat was less, the fish had no significant pathological characteristics, and the overall health was good. The absolute number of eggs laid in each experimental group was extremely significant (P<0.01). The T1, T2, and T3 groups increased by 31.70%, 73.11%, and 105.06% respectively compared with the C4 group. Compared with the T1-T5 groups, the absolute number of eggs laid in the C1-C3 groups decreased significantly, which shows that when the composition does not contain NCG, motherwort or compound amino acids, the effect of improving the reproductive performance of fish decreases, indicating that the combination of NCG, motherwort, compound amino acids, compound vitamins, licorice extracts and astragalus polysaccharides as the composition of the present invention can synergistically improve the reproductive performance of fish.

[0061] Table 3 Effects of different concentrations of compositions for improving fish reproduction performance on the reproduction process of largemouth bass

[0062]

[0063] 2.3 Effects of the composition for improving fish reproductive performance on the hatching rate and hatching deformity rate of largemouth bass

[0064] As shown in Table 4, the hatching rate of fertilized eggs of largemouth bass in each group of this test is all>70%, which is consistent with the hatching rate of fertilized eggs in outdoor ponds during the same period of the test. Among them, the hatching rate of T3 group is significantly higher than that of C4 control group (P<0.05), and the hatching rate reaches 82.22%. The hatching rate of T2 group is also 9.35% higher than that of C4 control group. The deformity rate of each group of the test is all<3%, with no significant difference (P>0.05), but the hatching deformity rate of T0 control group is slightly higher than that of each experimental group. This value is consistent with the hatching deformity rate of largemouth bass fertilized eggs produced in outdoor soil ponds during the same period and not participating in the test breeding. The above data illustrate that the composition for improving the reproductive performance of fish of the present invention has no adverse reaction to the development of largemouth bass fertilized eggs.

[0065] Table 4 Effects of different concentrations of compositions for improving fish reproduction performance on fertilized eggs of largemouth bass

[0066]

[0067] In summary, the present invention can synergistically promote the gonadal development of largemouth seabass by combining NCG, motherwort, compound amino acids, compound vitamins, licorice extract and astragalus polysaccharide into a composition of the present invention, advance the spawning time, increase the number of eggs laid and the number of fertilized eggs and the hatching rate of fertilized eggs, and at the same time will not cause an increase in the deformity rate of fertilized eggs, which can effectively improve the gonadal development of fish and enhance its reproductive performance.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for improving fish reproductive performance, characterized in that: Each 1000 parts by weight of the composition comprises the following components in parts by weight: 25-150 parts of N-carbamylglutamic acid, 100-400 parts of motherwort, 50-300 parts of complex amino acids, 0.5-5 parts of complex vitamins, 10-100 parts of licorice extract and 10-100 parts of astragalus polysaccharide; the fish is largemouth bass; Each 1kg of the composite amino acid comprises 2.4g of L-proline, 2.4g of L-serine, 4.8g of L-alanine, 8.5g of L-isoleucine, 11.8g of L-leucine, 6.0g of L-aspartic acid, 0.6g of L-tyrosine, 1.8g of L-glutamic acid, 12.8g of L-phenylalanine, 12.0g of L-arginine hydrochloride, 10.3g of L-lysine hydrochloride, 8.6g of L-valine, 6.0g of L-threonine, 6.0g of L-histidine hydrochloride, 2.2g of L-tryptophan, 5.4g of L-methionine, 0.2g of L-cystine and 18.2g of glycine; Every 1 gram of the complex vitamin includes 1.5 mg of vitamin A, 3 mg of vitamin B1, 3.4 mg of vitamin B2, 3 mg of vitamin B4, 0.009 mg of vitamin B12, 90 mg of vitamin C, 0.01 mg of vitamin D, 30 mg of vitamin E, 0.4 mg of folic acid, 10 mg of pantothenic acid and 0.03 mg of biotin.

2. The composition for improving fish reproduction performance as claimed in claim 1, characterized in that: Every 1000 parts by weight of the composition comprises the following components in parts by weight: 50-100 parts of N-carbamylglutamic acid, 150-300 parts of motherwort, 100-200 parts of complex amino acids, 1.5-2.5 parts of complex vitamins, 25-75 parts of liquorice extract and 20-80 parts of astragalus polysaccharide.

3. The composition for improving fish reproduction performance as claimed in claim 2, characterized in that: Every 1000 parts by weight of the composition comprises the following components in parts by weight: 50 parts of N-carbamylglutamic acid, 200 parts of motherwort, 130 parts of complex amino acids, 2 parts of complex vitamins, 50 parts of liquorice extract and 30 parts of astragalus polysaccharide.

4. A composition for improving fish reproduction performance as claimed in any one of claims 1 to 3, characterized in that: The composition for improving fish reproduction performance also includes starch supplemented to a total weight of 1000 parts.

5. Use of the composition for improving the reproductive performance of fish as claimed in any one of claims 1 to 3 in the preparation of feed for improving the reproductive performance of fish, wherein the fish is largemouth bass.

6. Use of the composition for improving fish reproductive performance as claimed in any one of claims 1 to 3 in the preparation of feed for promoting fish spawning, wherein the fish is largemouth bass.

7. The use according to claim 5 or 6, characterized in that: The composition for improving fish reproductive performance accounts for 0.72-3.6% of the total weight of the feed for improving fish reproductive performance or the feed for promoting fish spawning.

Images