A daoyin tranquilizing paste and a preparation method thereof

Abstract

The application belongs to the technical field of medicine manufacturing, and discloses a through-the-du-to-soothe-the-nerves paste and a preparation method thereof. The raw material composition of the through-the-du-to-soothe-the-nerves paste comprises cornu cervi 10 g, zizyphus jujuba mill 30 g, notopterygium 10 g, panax 10 g, pueraria 30 g, astragalus 30 g, cassia bark 10 g, radix paeoniae alba 20 g, citrus grandis 10 g, poria cocos 20 g, licorice 6 g, radix scutellariae 6 g, and malt 20 g. The optimal preparation process parameters are as follows: water addition amount 1200 ml, soaking time 70 min, and decocting time 180 min. The application can relieve the low mood of patients, is used for treating insomnia, pain, and fatigue caused by depression, and blocks the development of depression. The application has the advantages of flexible prescription, individualized prescription, and temporary adjustment according to individual conditions, time, and place. The paste also has the characteristics of wide application range, main and secondary considerations, combination of treatment and prevention, and suitability for various complex diseases.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of medicine manufacturing, and particularly relates to a dredging-Du and soothing-nerves paste and a preparation method thereof. BACKGROUND

[0002] Depression is a disease characterized by long-term low mood, decreased interest, and sleep disorders. Due to the lack of obvious early symptoms, it is easily ignored. At present, it presents the characteristics of high incidence, high disability rate, and high mortality rate, and seriously endangers the health of patients with depression.

[0003] The TCM mind-body integrated view has its unique treatment advantages. TCM has been continuously deepened in the research on depression and plays a role in treatment and prevention. The present application is based on the theoretical basis of "Yangqi is the essence of nourishing the spirit and the softness of nourishing the tendon" in Huangdi Neijing, and uses dredging-Du and soothing-nerves as the treatment method. The Yangqi of Du meridian is dredged so that the Yangqi is no longer depressed, thereby relieving the symptoms of low mood in patients. At the same time, it has significant therapeutic effect on insomnia, pain, and fatigue caused by depression, and blocks the development of depression.

[0004] At present, antidepressants mainly improve or regulate the function of monoamine neurotransmitters and receptors in the central nervous system to improve the symptoms of patients with depression. They are mainly divided into selective serotonin reuptake inhibitors (SSRIs), selective serotonin and norepinephrine reuptake inhibitors (SNRIs), norepinephrine and specific serotonin antidepressants (NaSSA), norepinephrine and dopamine reuptake inhibitors (NDRI), tricyclic antidepressants (TCA), and melatonin drugs. Although these antidepressants have certain therapeutic effect, they still have obvious adverse reactions, and have the disadvantages of low cure rate, high recurrence rate, and poor patient compliance.

[0005] TCM emphasizes the integrity of the body, and pays attention to the coordination and unity of man, nature, society, and psychology. In the treatment of depression, it is important to start from the whole. In the understanding of the pathogenesis, it is not only limited to the stagnation of liver qi, but also from the perspective of heart, spleen, kidney, lung, and Du meridian, and proposes the important role of Yangqi of five Zang organs in the treatment of depression. The dredging-Du and soothing-nerves paste is based on the theoretical basis of "Yangqi is the essence of nourishing the spirit and the softness of nourishing the tendon" in Huangdi Neijing, and uses dredging-Du and soothing-nerves as the treatment method. The Yangqi of Du meridian is dredged so that the Yangqi is no longer depressed, thereby relieving the symptoms of low mood in patients. At the same time, it has significant therapeutic effect on insomnia, pain, and fatigue caused by depression, and blocks the development of depression.

[0006] Through the above analysis, the problems and defects of the prior art are that the existing antidepressants have certain therapeutic effect, but still have obvious adverse reactions, and have the disadvantages of low cure rate, high recurrence rate, and poor patient compliance. SUMMARY

[0007] In view of the problems in the prior art, the present application provides a Tongdu Anshen ointment and a preparation method thereof.

[0008] The present application is implemented in a kind of Tongdu Anshen ointment, the raw material composition of Tongdu Anshen ointment is antler glue 10g, 30g of Chinese date seed, 10g of Qianghuo, 10g of ginseng, 30g of radix puerariae, 30g of radix astragali, 10g of cassia twig, 20g of white peony root, 10g of dried tangerine or orange peel, 20g of poria cocos, 6g of licorice, 6g of radix scrophulariae, 20g of malt.It is soaked in 1200mL water after the above-mentioned raw material is added 70min, and is boiled at 100℃, normal pressure 180min, and the filtrate is concentrated after being concentrated, and is placed on slow simmer in the antler glue of melt and open in gentle fire, until the paste yield is 29.56%~41.78% of the paste of clear paste.

[0009] Another purpose of the present application is to provide a kind of Tongdu Anshen ointment preparation method, including: formula-soaking-boiling-concentrating-collecting paste-cooling paste-sterilization-refrigeration.

[0010] S1, formula: according to prescription proportion, the medicinal material is weighed, wrapped with double gauze, and tied fast with rope;

[0011] S2, soaking: the medicine (except antler glue) wrapped with gauze is placed in the clean stainless steel pot of appropriate volume together, and the medicine is soaked in appropriate amount of water, so that it is fully absorbed and swells, and is soaked for 70min;

[0012] S3, boiling: the medicine after soaking is placed in decocting machine, and is boiled at 100℃, normal pressure 180min, and is deposited and filtered after standing, to obtain filtrate;

[0013] S4, concentration: the filtered medicinal juice is poured into stainless steel pot, heated using electromagnetic oven, and concentrated, which can be boiled first using fierce fire, to accelerate the evaporation of water in medicinal liquid, and the upper layer of medicinal liquid is removed using wooden spoon at any time, and when the medicinal juice is slowly thickened, gentle fire is used for further concentration, which needs to be stirred constantly to avoid paste, pot sticking, and until the medicinal juice is "not scattered on paper" for degree, which can stop concentration;

[0014] S5, paste collection: the antler glue of steam melt and open is poured into the above-mentioned concentrated paste, slowly simmered on gentle fire, and stirred constantly using wooden spatula, until it can be pulled into flag or water droplet into bead;

[0015] S6, paste cooling: the collected paste is cooled in paste collection room, and the temperature is controlled below 20℃, and the relative humidity is controlled at 55%~75%, and is placed overnight, and is fully cooled;

[0016] S7, sterilization: the cooled paste is placed in ultraviolet sterilization room, and is fully sterilized;

[0017] S8, refrigeration: after sterilization, the plaster is loaded into a clean porcelain container, placed in a refrigeration room, and the temperature is controlled at 2-8℃.

[0018] In combination with the above technical solutions and the technical problems solved, the technical solutions of the application have the following advantages and positive effects:

[0019] First, considering the efficacy and long-term clinical use, the application is prepared as a decoction. Traditional ointment is one of eight dosage forms in traditional Chinese medicine, namely pill, powder, ointment, pill, wine, dew, soup, and tablet. Its advantages include flexible prescription, quantity of body, and body constitution, including normal constitution, deficiency constitution, excess constitution, and deficiency-excess mixed constitution. It is suitable for individual conditions and can be adjusted according to different conditions. The ointment also has the characteristics of wide compatibility, primary and secondary compatibility, combination of treatment and supplementation, and suitability for various complex diseases. Moreover, it has the advantages of slow and stable drug effect, large drug concentration, small volume, easy preservation, and convenient use compared with traditional soup. Through optimization of the preparation process parameters and preliminary evaluation of the pharmacological effects of the ointment, the application lays a solid foundation for the development of Tongdu Anshen Ointment as a medical institution preparation and a new Chinese medicine.

[0020] Second, in the application, deer horn glue and jujube seed are used as the monarch drugs to achieve the effect of dredging the governor vessel and calming the mind. Qianghuo and ginseng are used to guide the spine and dredge the governor vessel. Huangqi, Gegen, Guizhi, and Baishao are used to tonify qi, consolidate the exterior, raise yang, and relieve muscle and tendon. Huangqi and Maidan are used to soothe the liver, and Gancao is used to harmonize all drugs. The ointment regulates the ying and wei, promotes sleep, dredges the governor vessel, supplements yang qi, supplements liver blood, strengthens bones and tendons, and dredges the yang qi of the governor vessel, so that the yang qi is no longer stagnant, thereby relieving the symptoms of emotional depression, insomnia, pain, and fatigue caused by depression, and blocking the development of depression.

[0021] Third, the technical solutions of the application solve the technical problems that people have been eager to solve but have failed to achieve success:

[0022] Today, most people are suffering from physical damage caused by professional overuse, especially prolonged sitting. As the main load-bearing body structure of the body in a sitting position, prolonged sitting will lead to fatigue of the soft tissues of the spinal system, excessive pressure or uneven force on the internal bones and joints, causing imbalance of the spinal support system. The circulation of skin, muscle, tendon, bone, and marrow blood around the spine is blocked, causing neck, waist, and back pain and a series of complex physical and mental symptoms. Repeated clinical trials have confirmed that the application has a therapeutic effect on the current epidemic diseases: depression, cervical spondylosis, insomnia, professional low back pain, and chronic fatigue syndrome.

[0023] Fourth, the significant technical progress of the preparation method provided by the application is reflected in several key aspects:

[0024] 1. Precise formulation ratios and medicinal material processing: By precise measurement and specific medicinal material processing methods (such as the evaporation and sublimation of deer antler glue), the effective components of the plaster are fully released, improving the product's efficacy.

[0025] 2. Controlled preparation environment and parameters: Detailed preparation steps, such as soaking time, decoction temperature and time, and concentration endpoint judgment ("dropping on paper does not spread out"), are based on a deep understanding of drug properties and reaction mechanisms. This strict control of process parameters ensures the consistency of the quality and efficacy of the plaster.

[0026] 3. Innovative processing technology: The combination of modern equipment (such as electric stoves and ultraviolet sterilization chambers) with traditional processes not only preserves the traditional essence of traditional Chinese medicine, but also improves production efficiency and product safety.

[0027] 4. Environmental and temperature control: Strict control of environmental temperature and humidity and the use of ultraviolet sterilization during the cooling and sterilization stages of the plaster not only ensure the stability and long-term preservation of the plaster, but also greatly reduce the risk of microbial contamination.

[0028] 5. Quality control of the final product: Through the cooling, sterilization, and refrigeration steps at the end of the preparation process, the safety and effectiveness of the plaster are further ensured, improving the product's market competitiveness.

[0029] These technological advancements not only improve the preparation efficiency and product quality of Tongdu Anshen Plaster, but also demonstrate the progress of traditional Chinese medicine preparation technology in modernization and standardization, contributing to the wider application of traditional Chinese medicine in modern medical and health fields. BRIEF DESCRIPTION OF DRAWINGS

[0030] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments of the present application. Obviously, the drawings described below are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0031] Figure 1 is a flow chart of the preparation method of Tongdu Anshen Plaster provided by the embodiments of the present application;

[0032] Figure 2 is a comparison chart of the body weight of each group of rats; compared with the blank group: P<0.01; compared with the model group: * P<0.01; compared with the TDAS low-dose group: P<0.01; compared with the Xiaoyao San group: * P<0.01;

[0033] Figure 3is a comparison chart of sugar water preference rate of rats in each group provided by the embodiment of the present application; compared with the blank group: P<0.01; compared with the model group: * P<0.01;

[0034] Figure 4 is a comparison chart of open field experiment of rats in each group provided by the embodiment of the present application; compared with the blank group: P<0.01; compared with the model group: * P<0.01; compared with the Xiaoyaosan group: P<0.01; compared with the TDAS low-dose group: * P<0.01;

[0035] Figure 5 is a comparison chart of ATP concentration of rats in each group provided by the embodiment of the present application; compared with the blank group: P<0.01; compared with the model group: * P<0.01; compared with the Xiaoyaosan group: P<0.01;

[0036] Figure 6 is a comparison chart of mitochondrial respiratory chain complex level of rats in each group provided by the embodiment of the present application; compared with the blank group: P<0.01; compared with the model group: * P<0.01; compared with the Xiaoyaosan group: P<0.01; compared with the TDAS low-dose group: * P<0.01;

[0037] Figure 7 is a comparison chart of cytochrome C protein expression of rats in each group provided by the embodiment of the present application; compared with the blank group: P<0.01; compared with the model group: *P<0.01; compared with the TDAS low-dose group: *P<0.01. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical scheme and advantages of the present application clearer and more apparent, the present application will be further described in detail below with examples. It should be understood that the specific examples described herein are only used to explain the present application and do not limit the present application.

[0039] Example 1:

[0040] The preparation process of the Tongdu Anshen Paste follows the following steps:

[0041] 1. Formulation: accurately weigh the antler glue, jujube seed, nuan-guo, ginseng, radix puerariae, cassia twig, white peony root, dried tangerine or orange peel, poria cocos, licorice, radix scutellariae and malt according to the prescription ratio. Among them, the antler glue needs to be handled separately due to its hard texture, and the rest of the medicinal materials are wrapped with double-layer gauze and tied tightly.

[0042] 2. Soaking: Place the medicinal materials (except for cornu cervi) wrapped in gauze into a clean stainless steel pot, and add enough water to ensure that the medicinal materials are fully immersed. Soak for 70 minutes to allow the medicinal materials to fully absorb water and expand.

[0043] 3. Decoction: Move the soaked medicinal materials into a decoction machine, and set the temperature to 100°C. Decoction for 180 minutes under normal pressure. Then let it stand to allow the impurities in the medicinal liquid to naturally precipitate, and obtain the pure filtrate by filtration.

[0044] 4. Concentration: Pour the filtrate into a stainless steel pot, and first use high heat to quickly heat the medicinal liquid to rapidly evaporate the water. Stir constantly and remove the scum during this process. When the medicinal liquid becomes thick, switch to low heat and continue to simmer while stirring to prevent burning or sticking to the pot, until it reaches the state of "dropping on paper does not spread".

[0045] 5. Collecting the paste: Add the previously treated cornu cervi to the concentrated paste, and continue to simmer with low heat while constantly stirring with a wooden spatula until the medicinal paste can be pulled into a flag shape or reaches the state of "water droplets into beads".

[0046] 6. Cooling the paste: Place the prepared medicinal paste in a paste collection room, control the room temperature below 20°C, and the relative humidity between 55% and 75%, and let it stand overnight to allow the medicinal paste to fully cool.

[0047] 7. Sterilization: Place the cooled medicinal paste in a UV sterilization room for thorough sterilization treatment to ensure the hygiene and safety of the medicinal paste.

[0048] 8. Refrigeration: Place the sterilized medicinal paste in a clean porcelain container, and place it in a refrigeration room to control the temperature between 2 and 8°C to maintain the stability and efficacy of the medicinal paste.

[0049] Example 2:

[0050] The preparation of Tongdu Anshen Paste is carried out according to the following steps:

[0051] 1. Formulation: Accurately weigh the required medicinal materials such as cornu cervi, Suanzaoren, Niuweishu, Renshen, Gegen, Huangqi, Guizhi, Baishao, Chenpi, Fuling, Gancao, Huangqin, and Maiya according to the proportions described in the claims.

[0052] 2. Soaking: Wrap the medicinal materials (except for cornu cervi) in gauze, and place them in a clean stainless steel pot with appropriate volume, and add enough water for soaking. Soak for 1 hour to ensure that the medicinal materials fully absorb water and expand.

[0053] 3. Decoction: Pour the soaked medicinal materials and the soaking liquid into a decoction machine, and set the temperature to 105°C. Decoction for 2 hours under normal pressure. After standing, allow the impurities to naturally precipitate, and obtain the clear filtrate by filtration.

[0054] 4. Concentration: Pour the filtrate into a stainless steel pot and start heating rapidly with a large fire while continuously stirring to remove scum. When the medicinal liquid is concentrated to a certain extent, turn to slow fire and continue to simmer with constant stirring until the medicinal paste becomes very thick, reaching the standard of "flag hanging" or "water dripping into beads".

[0055] 5. Collecting paste: Slowly pour the pre-melted deer horn glue into the concentrated paste, continue to simmer with small fire, and continuously stir until the medicinal paste reaches the desired texture and viscosity.

[0056] 6. Cooling paste: Move the prepared medicinal paste to the cooling paste room, control the room temperature at about 18℃, and keep the relative humidity at 60%-70%, stand overnight to allow the medicinal paste to cool and solidify.

[0057] 7. Sterilization: Put the cooled medicinal paste into a special sterilization room and use ultraviolet light for thorough sterilization to ensure the hygiene and safety of the medicinal paste.

[0058] 8. Refrigeration: Put the sterilized medicinal paste into a clean and dry porcelain or glass container, seal it and put it into the refrigeration room for storage, control the temperature at about 4℃, to prolong the shelf life of the medicinal paste and maintain its medicinal effect.

[0059] In view of the problems existing in the prior art, the present application provides a Tongdu Anshen paste and a preparation method thereof, which will be described in detail below in conjunction with the accompanying drawings.

[0060] The following are six specific examples of Tongdu Anshen paste, each with slight adjustments in raw material composition to meet the needs of different populations or different conditions:

[0061] Example 1:

[0062] The raw material composition of Tongdu Anshen paste is: deer horn glue 12g, jujube seed 35g, nuxvomica 8g, ginseng 12g, kudzu root 28g, astragalus 35g, cassia twig 8g, white peony root 22g, dried tangerine or orange peel 12g, tuckahoe 25g, licorice 5g, yellow pagoda tree 8g, wheat germ 22g. This example makes slight adjustments in the amount of raw materials to enhance its effect of soothing the nerves.

[0063] Example 2:

[0064] The raw material composition of Tongdu Anshen paste is: deer horn glue 8g, jujube seed 28g, nuxvomica 12g, ginseng 8g, kudzu root 32g, astragalus 28g, cassia twig 12g, dried tangerine or orange peel 10g, tuckahoe 20g, white peony root 18g, licorice 8g, yellow pagoda tree 4g, wheat germ 18g. This example focuses on adjusting the balance between medicinal materials to achieve better comprehensive therapeutic effect.

[0065] Example 3:

[0066] The raw material composition of the Tongdu Anshen paste is: antler glue 10g, jujube kernel 25g, nuxvomica 15g, ginseng 15g, kudzu root 25g, astragalus 40g, cassia twig 6g, white peony root 25g, dried tangerine or orange peel 9g, tuckahoe 25g, licorice 10g, scaphulae 5g, and barley malt 15g. The embodiment is for specific groups of people, and the effect of tonifying qi is enhanced by increasing the amount of astragalus.

[0067] Embodiment 4:

[0068] The raw material composition of the Tongdu Anshen paste is: antler glue 9g, jujube kernel 32g, nuxvomica 9g, ginseng 11g, kudzu root 35g, astragalus 25g, cassia twig 11g, white peony root 19g, dried tangerine or orange peel 9g, tuckahoe 21g, licorice 7g, scaphulae 7g, and barley malt 25g. The embodiment pays more attention to balancing the amount of various medicinal materials to achieve the effect of overall regulation.

[0069] Embodiment 5:

[0070] The raw material composition of the Tongdu Anshen paste is: antler glue 10g, jujube kernel 40g, nuxvomica 5g, ginseng 18g, kudzu root 20g, astragalus 38g, cassia twig 5g, white peony root 28g, dried tangerine or orange peel 6, tuckahoe 18g, licorice 4g, scaphulae 10g, and barley malt 10g. The embodiment increases the amount of jujube kernel and ginseng to enhance the effect of nourishing the heart and tonifying qi.

[0071] Embodiment 6:

[0072] The raw material composition of the Tongdu Anshen paste is: antler glue 15g, jujube kernel 20g, nuxvomica 18g, ginseng 5g, kudzu root 40g, astragalus 20g, cassia twig 15g, white peony root 15g, dried tangerine or orange peel 15, tuckahoe 30g, licorice 9g, scaphulae 9g, and barley malt 28g. The embodiment adjusts the amount of some medicinal materials while keeping the overall efficacy unchanged to adapt to different body types and needs of people.

[0073] The amount of raw materials in the above embodiments is the recommended amount, and actual use can be adjusted appropriately according to individual conditions and the need for treatment. At the same time, when preparing the Tongdu Anshen paste, the purity and quality of the raw materials should be ensured, and the traditional preparation process should be followed to ensure the exertion of its efficacy.

[0074] The preparation method of the Tongdu Anshen paste provided by the embodiment of the present application is as shown in Figure 1 .

[0075] The raw material composition of the Tongdu Anshen paste provided by the present application is: antler glue 10g, jujube kernel 30g, nuxvomica 10g, ginseng 10g, kudzu root 30g, astragalus 30g, cassia twig 10g, white peony root 20g, dried tangerine or orange peel 10, tuckahoe 20g, licorice 6g, scaphulae 6g, and barley malt 20g.

[0076] First, according to the prescription proportion of medicinal materials, double gauze is wrapped and tied with a rope; the medicine wrapped with gauze (except for deerhorn glue) is put into a clean stainless steel pot with appropriate volume, 1200 mL of water is added for soaking for 70 min, and it is fully absorbed and expanded; it is put into a decoction machine, decocted at 100℃ under normal pressure for 180, and after standing, it is precipitated and filtered to obtain the filtrate; the filtered medicinal juice is poured into a stainless steel pot, heated with an electromagnetic oven, concentrated, first decocted with a strong fire to accelerate the evaporation of water in the medicinal liquid, and the upper layer of the medicinal liquid is removed at any time with a wooden spoon, when the medicinal juice becomes thick, the fire is changed to a weak fire for further concentration, at this time, constant stirring is needed to avoid pasting and sticking to the pot, until the medicinal juice is "dropped on paper without spreading" for the degree, at this time, the concentration can be stopped; the evaporated and melted deerhorn glue is poured into the concentrated paste mentioned above, slowly fried on a weak fire, and constantly stirred with a wooden spatula until it can be pulled into a flag or water droplets into a bead; the collected medicinal paste is cooled in the collection room, and the temperature is controlled below 20℃, the relative humidity is controlled at 55%~75%, and it is placed overnight to fully cool; the cooled medicinal paste is placed in a ultraviolet sterilization room for full sterilization; the sterilized medicinal paste is put into a clean porcelain container, placed in a refrigeration room, and the temperature is controlled at 2~8℃.

[0077] Analysis of composition and principle:

[0078] Deerhorn glue 10g, jujube kernel 30g, nitraria 10g, ginseng 10g, radix puerariae 30g, cassia bark 10g, white peony root 20g, tangerine peel 10, tuckahoe 20, licorice 6g, radix scrophulariae 6g, and malt 20g.

[0079] Deerhorn glue, jujube kernel (commonly play the role of soothing the du channel and calming the mind).

[0080] Nitraria and ginseng (guide the medicine to the spine and the du channel).

[0081] Radix puerariae, radix puerariae, cassia bark, white peony root, tangerine peel, tuckahoe, radix scrophulariae, and malt (promote sleep, dredge the liver, and strengthen the liver blood and bones).

[0082] Licorice (harmonize all medicines).

[0083] The du channel soothing and calming paste has been repeatedly clinically confirmed to have good effect on the current epidemic diseases: cervical spondylosis, insomnia, professional waist and back pain, depression, and fatigue sub-health (chronic fatigue syndrome). The formula regulates the ying and wei, promotes sleep, dredges the du channel, supplements yang qi, supplements liver blood, and strengthens bones. The following are application examples:

[0084] Case one: heart and chest tightness, Rong, female, 33 years old, nurse

[0085] Chest tightness for two years, dizziness, irritability, sleep less, daytime lethargy, fatigue, depression, acne on the forehead, significantly reduced menstrual flow in the past three years. Tender red tongue with thin moss, pulse is deep and fine.

[0086] Prescription: Tongdu Anshen Plaster plus Gardenia 6g Mulberry Bark 15g Rose Flower 6g Chest and back chiropractic. Second week of referral complained of feeling comfortable the day of treatment.

[0087] Case two: professional low back pain, Wei, male, 30 years old, coffee, game player persistent back pain for six months, go to bed late, fatigue, lethargy, loose stool. Pale tender tongue with thin moss, pulse is thin and wiry.

[0088] Prescription: Tongdu Anshen Plaster plus acupuncture Jiazheng points, along the long needle along the spine and supplemented by chest chiropractic techniques. Two weeks later, the pain was greatly relieved, sleep was improved, and the spirit was improved.

[0089] Experimental study: According to the experimental design, 48 SD adult male rats, weighing about 200g, were randomly divided into 6 groups, namely normal control group, depression model group, Tongdu Anshen Plaster (hereinafter referred to as TDAS) low dose group, TDAS medium dose group, TDAS high dose group, Xiaoyao San group, 8 rats in each group. Chronic unpredictable mild stress (CUMS) was used to prepare the depression model of rats. The concentration of TDAS low dose was 0.406g·mL -1 , the concentration of TDAS medium dose was 0.812g·mL -1 , the concentration of TDAS high dose was 1.62g·mL -1 , the intragastric volume of each rat was 1mL·100g -1 . The concentration of Xiaoyao San was 0.59g·mL -1 , the intragastric volume was 1mL·100g -1 . The body mass, and the body mass index, sugar water preference test, open field test and other behavioral indicators of rats were observed, and the purified mitochondria of hippocampal tissue were extracted. The ATP content was detected by enzyme-linked immunosorbent assay (ELISA), the activities of mitochondrial respiratory chain enzyme complexes I, II, III and IV were detected by biochemical detection method, and the changes of mitochondrial cytochrome C expression were detected by Western blot (WB) method. To clarify the related mechanism of mitochondrial damage in depression model rats and the intervention effect of Tongdu Anshen Plaster.

[0090] 1Effect on body weight of depression model rats

[0091] Compared with the blank group, the weight growth rate of the model group was slower, and the difference was statistically significant (P<0.01). After treatment, compared with the model group, the body weight of each treatment group increased significantly (P<0.01), and the body weight of the TDAS high-dose group was significantly higher than that of the TDAS low-dose group and the Xiaoyao San group (P<0.01). See Figure 2 .

[0092] 2Effects on sucrose preference test of depressive model rats

[0093] Compared with the blank group, the sucrose preference rate of the model group decreased significantly (P<0.01). After treatment, compared with the model group, the sucrose preference rate of each treatment group increased significantly (P<0.01), with significant differences. See Figure 3 .

[0094] 3Effects on open field test of depressive model rats

[0095] Compared with the blank group, the total distance of the model group decreased, and the resting time increased (P<0.01). After treatment, compared with the model group, the total distance of each treatment group increased (P<0.01), and the total distance of the TDAS high-dose group was significantly higher than that of the TDAS low-dose group (P<0.01), and the resting time of the TDAS high-dose group was significantly shorter than that of the Xiaoyao San group (P<0.01). See Figure 4 .

[0096] 4Effects on hippocampal ATP content of depressive model rats

[0097] Compared with the blank group, the content of hippocampal mitochondrial ATP in the model group decreased significantly (P<0.01). After treatment, compared with the model group, the content of hippocampal mitochondrial ATP in the Xiaoyao San group and the TDAS groups increased significantly (P<0.01), and the content of hippocampal mitochondrial ATP in the TDAS high-dose group was significantly higher than that in the Xiaoyao San group (P<0.01). The results showed that the content of hippocampal mitochondrial ATP in depressive model rats decreased. After drug intervention, TDAS can increase the content of hippocampal mitochondrial ATP in depressive model rats to a certain extent, and the improvement effect is better than that of Xiaoyao San. See Figure 5 .

[0098] 5Effects on mitochondrial respiratory chain complex level of depressive model rats

[0099] Compared with the model group, the levels of mitochondrial respiratory chain complex I-IV in the hippocampus of the model group were significantly decreased (P<0.01). Compared with the model group, the levels of mitochondrial respiratory chain complex I-IV in the hippocampus of each treatment group were significantly increased (P<0.01). Compared with the Xiaoyao San group, the level of mitochondrial respiratory chain complex I in the hippocampus of the TDAS high-dose group was significantly increased (P<0.01). Compared with the TDAS low-dose group, the levels of mitochondrial respiratory chain complex II, III and IV in the hippocampus of the TDAS high-dose group were significantly increased (P<0.01). Figure 6 .

[0100] 6Effects of TDAS on the expression of cytochrome C in the hippocampus of the depression model rats

[0101] Compared with the model group, the expression of cytochrome C in the hippocampus of the model group was significantly increased (P<0.01). After the treatment, compared with the model group, the expression of cytochrome C in the hippocampus of the Xiaoyao San group, the TDAS low-dose, medium-dose and high-dose groups was significantly decreased (P<0.01), and the effect of the TDAS high-dose group was more obvious than that of the TDAS low-dose group (P<0.01). The results of the present study suggest that chronic stress can cause the increase of cytochrome C release from the hippocampal neurons, and TDAS can inhibit the release of cytochrome C and thus reduce the apoptosis of hippocampal neurons. See Figure 7 .

[0102] The above merely illustrates the specific embodiments of the present application, but the protection scope of the present application is not limited thereto, and any modification, equivalent replacement and improvement made by any person skilled in the art within the technical range disclosed by the present application and in the spirit and principle of the present application shall be covered within the protection scope of the present application.

Claims

1. A calming and soothing ointment for treating depression, characterized in that, Its ingredients are deer antler glue, jujube seed, notopterygium root, ginseng, kudzu root, astragalus root, cinnamon twig, white peony root, licorice root, scutellaria root, and malt. The dosage of deer antler glue is 10g, jujube seed is 30g, notopterygium root is 10g, ginseng is 10g, kudzu root is 30g, astragalus root is 30g, cinnamon twig is 10g, white peony root is 20g, tangerine peel is 10g, poria cocos is 20g, licorice root is 6g, scutellaria root is 6g, and malt is 20g.

2. A method for preparing the Tongdu Anshen Ointment for treating depression as described in claim 1, characterized in that, The method involves mixing deer antler glue, jujube seed, notopterygium root, ginseng, kudzu root, astragalus root, cinnamon twig, white peony root, tangerine peel, poria cocos, licorice root, scutellaria root, and malt in the proportions described in claim 1, and processing them into a paste using traditional preparation techniques.

3. A method for preparing the Tongdu Anshen Ointment for treating depression as described in claim 2, characterized in that, include: Formula - Soaking - Boiling - Concentration - Thickening - Cooling - Sterilization - Refrigeration; S1, Formula: Weigh the medicinal materials according to the prescription ratio, wrap them in double-layered gauze, and tie them securely with a rope; S2, Soaking: Place all the medicinal materials except the deer antler glue, wrapped in gauze, into a clean stainless steel pot of appropriate volume, add an appropriate amount of water to moisten the medicinal materials, allow them to fully absorb and expand, and soak for 70 minutes. S3, decoction: Put the soaked herbs into the decoction machine and decoct at 100℃ and normal pressure for 180 minutes. After standing, let it settle and filter to obtain the filtrate. S4, Concentration: Pour the filtered medicinal juice into a stainless steel pot and heat it with an induction cooker to concentrate it. First, boil it over high heat to accelerate the evaporation of water in the medicinal liquid, and remove the foam on the surface of the medicinal liquid with a wooden spoon at any time. When the medicinal juice slowly thickens, turn to low heat to further concentrate it. At this time, it is necessary to stir constantly to avoid gelatinization and sticking to the pot, until the medicinal juice "does not spread when dripped on paper". Only then can the concentration be stopped. S5, Refining the Paste: Pour the melted deer antler glue into the above concentrated paste, simmer it over a low flame, and stir constantly with a wooden spatula until it can be stretched into a flag or dripped into a bead. S6, Cooling the plaster: Place the collected plaster in the plaster collection room and control the temperature below 20℃ and the relative humidity at 55%~75% overnight to allow it to cool completely. S7, Sterilization: Place the cooled plaster in an ultraviolet sterilization chamber for thorough sterilization; S8, Refrigeration: Place the sterilized ointment into a clean porcelain container, put it in the refrigerator, and control the temperature at 2~8℃.

4. The preparation method of Tongdu Anshen Ointment for treating depression as described in claim 3, characterized in that, Weigh out the amounts of the ingredients in the ointment according to the prescription, and use an L9 orthogonal array for experimental design. Combined with the results of single-factor experiments, we further investigated the effects of water volume, soaking time, and decoction time on the yield of ointment, and determined the optimal preparation process parameters to be: water volume 1200mL, soaking time 70min, and decoction time 180min.

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