Energy-saving livestock and poultry house purification device and method, new strain for purification and filter layer filler
By adopting an energy-saving purification device in the livestock and poultry breeding house, combining intermittent spraying method and modified polyurethane sponge filler, Bacillus Beles N4 as a deodorizing microorganism, the problem of high water consumption and power consumption in the prior art is solved, and efficient purification of odor and reduction of operating costs are achieved.
Patent Information
- Application Number
- CN202410568338.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-08
- Publication Date
- 2025-03-18
- Estimated Expiration
- 2044-05-08
AI Technical Summary
The existing livestock and poultry farmhouse purification devices consume higher water and electricity when dealing with odor, resulting in higher operating costs.
An energy-saving livestock and poultry farmhouse purification device is adopted. The device includes a vertically established body, a first-level filter layer, a spray piece and a second-level filter layer. Combined with intermittent spraying method and modified polyurethane sponge filler, Bacillus Beles N4 is used as a deodorizing microorganism for membrane treatment.
Through the design and method of the device, the consumption of water and electricity is significantly reduced, efficient purification of odor is achieved, and operating costs are reduced.
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Figure CN118526870B_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the field of livestock and poultry breeding waste gas treatment, and in particular to an energy-saving livestock and poultry breeding house purification device and method, a new strain for purification and a filter layer filler, which can effectively purify the end odor of the livestock and poultry house. Background Art
[0002] With the gradual development of intensive and large-scale livestock and poultry farming in my country, a large amount of harmful substances such as ammonia, particulate matter, and volatile organic compounds will be produced during the livestock and poultry farming process, which seriously affects the surrounding environment and causes a large number of complaints about stench or odor. The concept that green waters and green mountains are gold and silver mountains in my country has become more and more popular. In order to promote the sustainable development of livestock and poultry farming, terminal treatment technology or devices for harmful gases have gradually developed. The existing purification device generally uses a negative pressure fan to extract the odor from the pig house to the outside world, and then uses water or acid spray to make the odor fully contact with it, so that the odor is dissolved in water or acid to achieve the purpose of purification. It is currently the mainstream terminal exhaust gas purification device for livestock and poultry houses, especially pig farms. However, due to the huge ventilation volume and fast wind speed of livestock and poultry houses, under the action of huge ventilation volume, the spray water often turns into water mist and escapes into the atmosphere. Even if a return plate is set at the end of most equipment, the water consumption and power consumption are still high, resulting in high operating costs. Therefore, there is an urgent need for an energy-saving livestock and poultry house purification device and method that can reduce water consumption. Summary of the invention
[0003] A brief overview of the present application is provided below in order to provide a basic understanding of certain aspects of the present application. It should be understood that this overview is not an exhaustive overview of the present application. It is not intended to identify the key or important parts of the present application, nor is it intended to limit the scope of the present application. Its purpose is merely to provide certain concepts in a simplified form as a prelude to a more detailed description discussed later.
[0004] In view of the above-mentioned defects of the prior art, the purpose of this application is to provide an energy-saving livestock and poultry house purification device and method, a new strain for purification and a filter layer filler, which can achieve water-saving and energy-saving purification of odor in livestock and poultry houses through a special filtering structure and intermittent spraying method.
[0005] According to the first aspect of the present application, there is provided an energy-saving livestock and poultry house purification device, comprising: a vertically erected main body; a primary filter layer, a spray element, and a secondary filter layer arranged in sequence from bottom to top or in a horizontal direction in the upper part of the main body; a water guide plate and a sedimentation tank arranged in sequence from top to bottom in the lower part of the main body; an air inlet is provided on the side of the main body, the air inlet is located below or in front of the primary filter layer and above the water guide plate, and an air outlet is provided on the top of the main body.
[0006] According to a second aspect of the present application, there is provided an energy-saving livestock and poultry house purification method, which is implemented by the above-mentioned energy-saving livestock and poultry house purification device.
[0007] According to the third aspect of the present application, a new bacterial strain for purification of livestock and poultry houses is provided, namely Bacillus Velez N4, with the deposit number being CGMCC No.30274.
[0008] According to a third aspect of the present application, a filter layer filler for purifying livestock and poultry houses is provided, which is prepared by forming a polyurethane sponge with the Bacillus Velez N4.
[0009] Compared with the prior art, the beneficial effects of this application are:
[0010] 1) In the purification device of the present application, the filler of the secondary filter layer is a modified polyurethane sponge (such as a polyurethane sponge loaded with modified activated carbon and modified zeolite), which can increase its adsorption of odor, hydrogen sulfide, ammonia and volatile organic compounds (VOCs). At the same time, due to its large roughness, it can increase the amount of microbial biofilm. In addition, it has strong water absorption performance, can provide a sufficient humid environment, and save the use of spray water; on the other hand, due to its strong water absorption performance, it can replace the role of the return plate in the existing purification device, and combine the return plate with the secondary filter layer to save construction costs. At the same time, the primary filter layer can intercept a large amount of sticky particles (the sticky particles mainly come from the feces and feed dust of livestock and poultry breeding houses, with large viscosity. If not handled in time, the filler will be blocked quickly and the pressure drop will be increased), and it will be flushed into the pool below by the flushing force of the spray water to prevent the sticky particles from entering the secondary filter layer and causing blockage, increasing the pressure drop.
[0011] 2) The secondary filter layer filler in the device of the present application is formed by the deodorizing microorganism Bacillus velezensis N4. The deposit date of the new strain of Bacillus velezensis is April 7, 2024, the deposit number is CGMCC No. 30274, the classification name is: Bacillus velezensis N4, the deposit unit name is: General Microbiological Center of China Microbiological Culture Collection Administration Committee (referred to as CGMCC), the address is: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, zip code 100101. The strain has good ammonia reduction performance. After forming a film on a polyurethane sponge, it was sent to the Guangdong Provincial Microbiological Analysis and Testing Center (address: Building 66, No. 100 Xianlie Middle Road, Guangzhou) for determination, and its ammonia reduction performance was evaluated, and the removal rate was 95.1%.
[0012] 3) In the purification method of the present application, the intermittent spraying method is adopted through the above-mentioned purification device. Without reducing the purification efficiency, water sprays for a period of time, stops spraying for a period of time, and then continues spraying, and repeats this cycle, which can achieve the purpose of significantly reducing water and electricity consumption. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] The present application may be better understood by referring to the following description given in conjunction with the accompanying drawings, wherein the same or similar reference numerals are used throughout the drawings to represent the same or similar components. The accompanying drawings together with the following detailed description are included in and form a part of this specification, and are used to further illustrate the preferred embodiments of the present application and explain the principles and advantages of the present application.
[0014] in:
[0015] Figure 1 This is a schematic structural diagram of an energy-saving livestock and poultry breeding house purification device according to the first embodiment of the present application;
[0016] Figure 2 This is a schematic structural diagram of an energy-saving livestock and poultry breeding house purification device according to the second embodiment of the present application;
[0017] Figure 3 This is a schematic structural diagram of an energy-saving livestock and poultry breeding house purification device according to the third embodiment of the present application;
[0018] Figure 4 Shows photos of PP hollow balls without sponge and PP hollow balls with polyurethane sponge;
[0019] Figure 5 The ammonia removal rate of the strains with NH3 removal rate exceeding 50% in the strain screening experiment is shown;
[0020] Figure 6 A photograph of a colony of Bacillus velez N4 is shown;
[0021] Figure 7 The phylogenetic tree of Bacillus velez N4 is shown;
[0022] Figure 8 The schematic diagram of the plan layout of the pig house in Example 2 is shown;
[0023] Wherein, the accompanying drawings are marked as follows:
[0024] 1-odor; 2-clean gas; 3-primary filter layer; 4-spray water pipe; 5-secondary filter layer; 151-modified polyurethane sponge filler sphere; 6-nozzle; 7-interlayer; 71-first supporting mechanism; 72-second supporting mechanism; 8-inclined plate sedimentation tank; 9-sludge discharge pipe; 10-circulation pump timer switch controller; 11-flow meter; 12-purification tank; 13-spray circulation pump; 14-water replenishment; 15-agitator and motor; 16-EC sensor; 17-pH sensor; 18-ammonia nitrogen sensor; 19-reflux pipe; 20-MAP reaction tank; 21-PAM filling tank; 22-maturation tank; 23-MgCl2 dosing pipe; 24-Na2HPO4 dosing pipe; 25-PAM dosing pipe; 26-air inlet; 271-first drain valve; 272-second drain valve; 28-water guide plate.
[0025] Those skilled in the art should understand that the same reference numerals represent the same parts or parts with equivalent functions, and all drawings are only for the convenience of explaining the technical content of this application. The technical features such as the numbers used to constitute the optimal implementation, the positions of the parts, the relationship between the parts, and the sizes of the parts do not constitute a limitation on the technical solution itself, but should be extended to the entire field covered by the technical field. The elements and components in the drawings are only shown for simplicity and clarity, and are not necessarily drawn to scale; for example, the sizes of some elements and components in the drawings may be enlarged relative to other elements and components to help improve the understanding of the embodiments of this application. DETAILED DESCRIPTION
[0026] Exemplary embodiments of the present application will be described below in conjunction with the accompanying drawings. For the sake of clarity and conciseness, not all features of the actual implementation are described in the specification. However, it should be understood that many implementation-specific decisions must be made in the process of developing any such actual implementation in order to achieve the developer's specific goals, such as meeting those restrictions related to the device and the service, and these restrictions may vary from implementation to implementation. In addition, it should be understood that although the development work may be very complex and time-consuming, it is only a routine task for those skilled in the art who benefit from the content of this application.
[0027] It is also necessary to explain here that, in order to avoid obscuring the present application due to unnecessary details, only the device structure and / or processing steps closely related to the scheme of the present application are shown in the accompanying drawings, while other details that are not very relevant to the present application are omitted.
[0028] The present application provides an energy-saving livestock and poultry breeding house purification device, comprising: a vertically erected main body; a purification unit is provided at the upper part of the main body, comprising a primary filter layer, a spray element, and a secondary filter layer arranged in sequence from bottom to top, or a spray element, a primary filter layer, and a secondary filter layer arranged in sequence along the horizontal direction; a water treatment unit is provided at the lower part of the main body, comprising a water guide plate and a sedimentation tank arranged in sequence from top to bottom; an air inlet is provided on the side of the main body, the air inlet is located below or in front of the primary filter layer and above the water guide plate, and an air outlet is provided on the top or side of the main body.
[0029] Figure 1 The first exemplary structure of the energy-saving livestock and poultry house purification device of the present application is shown, including:
[0030] A body erected vertically;
[0031] In the upper part of the body, a primary filter layer 3, a spray element, and a secondary filter layer 5 are arranged in sequence from bottom to top;
[0032] In the lower part of the body, a water guide plate and a sedimentation tank 8 are arranged in sequence from top to bottom;
[0033] An air inlet 26 is provided on the side of the body, and the air inlet 26 is located below the primary filter layer 3 and above the water guide plate 28. An air outlet is provided on the top of the body.
[0034] When the purification device is in operation, the odor 1 enters the main body of the purification device through the air inlet 26, passes through the primary filter layer 3 with water mist sprayed by the spray unit, not only removes dust but also dissolves some soluble odors such as ammonia and soluble VOCs in water, and then continues to pass through the secondary filter layer 5 to further transform the adsorbed odor substances, and finally purifies the odor 1 and releases clean air 2 from the air outlet. The spray water descends and converges to the sedimentation tank 8 through the water guide plate, and the particles are separated and the spray water is recycled in time, reducing the pollutants of the odor substances on the particles dissolved in the spray water. At the same time, since the particles mainly come from the feed and feces in the livestock and poultry houses, they have greater viscosity, and can also prevent the particles from adhering to the primary filter layer, so as to avoid clogging the filter layer and increasing the pressure drop.
[0035] The following further describes the various components of the energy-saving livestock and poultry house purification device of the embodiment of the present application and their connection relationships and functional relationships.
[0036] The main body of the purification device is a hollow cavity inside, which is the main place for purification and dust removal. In the present application, the main body is set up vertically as a whole, which can make full use of the effect of gravity to make the spray water and the odor contact and react in countercurrent, and at the same time reduce the floor space. The main body can be roughly cylindrical, square or other shapes adapted to local conditions. The air inlet 26 of the purification device is generally arranged on the side of the main body, and specifically should be arranged below the primary filter layer 3, so that the odor 1 can be fully purified and dust-removed during the rising process. The air outlet of the purification device is generally arranged at the top of the main body, and when the odor 1 is fully purified and dust-removed during the rising process and becomes clean, it is discharged from the device.
[0037] A purification unit is provided in the upper part of the body of the purification device, including a primary filter layer 3, a spray element, and a secondary filter layer 5. Specifically, a primary filter layer 3, a spray element, and a secondary filter layer 5 are arranged in sequence from bottom to top in the upper part of the body.
[0038] The primary filter layer 3 is arranged above the air inlet and below the spray part. A water film formed by the water (spray water) sprayed from the spray part is attached to the primary filter layer. When the odor rises, the spray water fully contacts the countercurrent odor, so that some soluble odors such as ammonia and soluble VOCs are dissolved in the water, which plays a role in deodorization and purification. In addition, considering that livestock and poultry farming also produces a large amount of particulate matter, the design of the primary filter layer can also play a role in dust removal. In the embodiment of the present application, the primary filter layer 3 includes a first filler and a first supporting mechanism 71 for supporting the first filler. For example, the first filler can be made of common polypropylene plastic (PP plastic) with a porosity of about 97%. Commercially available polypropylene mesh fillers can be used, such as a purification plastic water curtain with a six-hole honeycomb structure formed by stacking filter screens; of course, the first filler can also be made of other materials, such as common volcanic rocks, ceramics, polyethylene (PE) and other plastics, which have the advantages of large porosity (up to 95%-97%), complex structure, corrosion resistance, large specific surface area, and low price. The first supporting mechanism 71 may be an ordinary filler supporting plate or other supporting devices, as long as it is sufficient to firmly support the first filler and can be ventilated. Preferably, the first supporting mechanism 71 is provided with ventilation holes with a diameter or side length of 5 cm to 10 cm.
[0039] The spraying part is arranged above the primary filter layer 3 to provide spray water, specifically, to spray water continuously or discontinuously to the primary filter layer 3 according to a preset treatment process. Generally, the spraying part includes a spraying water pipe 4 and a nozzle 6, the spraying water pipe 4 is connected to the external clean water pool 12, and the nozzle 6 is connected to the spraying water pipe 4, and its opening faces the primary filter layer 3. In order to better ensure the treatment effect, a plurality of nozzles 6 are generally arranged evenly. In a specific example, a spraying circulation pump 13 is arranged at the bottom of the clean water pool 12, and the spraying water pipe 4 is connected to the spraying circulation pump 13 through a pipeline, and a flow meter 11 and a circulation pump timing switch controller 10 are provided on the pipeline. In this way, a spraying system is formed. When the whole system is in operation, clean water is replenished into the clean water pool through the external water replenishment 14, the working state of the spraying circulation pump 13 is controlled by the circulation pump timing switch controller 10, and the spraying amount is monitored by the flow meter 11. In the preferred embodiment of the present application, the intermittent spraying method is mainly used. For example, water is first sprayed for 10 minutes, then the spraying is stopped for 50 minutes, the spraying is continued for 10 minutes, and then the spraying is stopped for 50 minutes. This cycle is repeated to achieve the purpose of reducing water and electricity consumption.
[0040] The secondary filter layer 5 is arranged above the spray part. After passing through the primary filter layer 3, the odor 1 undergoes a round of purification and dust removal, and continues to rise with water mist. When passing through the secondary filter layer 5, it undergoes deep treatment, that is, intercepting and absorbing the water mist brought from the primary filter layer 3, and continuing to absorb and transform hydrogen sulfide, ammonia and VOCs in the odor. In the embodiment of the present application, the secondary filter layer 5 includes a second filler and a second supporting mechanism 72 for supporting the second filler. Preferably, the second filler is a modified polyurethane sponge with a porosity of 95-97%, so as to ensure that the pressure drop of the equipment is not increased as much as possible and reduce the energy consumption of the fan. The polyurethane sponge has the advantages of high open porosity, large specific surface area, strong water absorption, high porosity, etc. Its porosity can reach 95%. On the one hand, the present application preferably uses a modified polyurethane sponge loaded with modified activated carbon and / or zeolite powder to increase the adsorption effect of the polyurethane sponge on hydrogen sulfide, ammonia and VOCs in the odor of livestock and poultry houses. In addition, there are biofilm deodorizing microorganisms on the polyurethane sponge, which can further convert the adsorbed ammonia. The strain is Bacillus velezensis N4, which has been deposited in the China General Microbiological Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences on April 7, 2024 (Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing), and the deposit number is: CGMCC No.30274; On the other hand, the polyurethane sponge has strong water absorption, and its water absorption capacity can be as high as 300%, which can absorb the water mist brought by the odor 1 from the primary filter layer 3, increase the filler humidity of the secondary filter layer 5, so that during the period of stopping spraying, the odor passing through can still be converted from the gas phase to the water phase, and further adsorbed and converted by the secondary filter layer 5. The second filler can be a whole piece of modified polyurethane sponge, but more preferably, the second filler includes a modified polyurethane sponge filler sphere 151 (its structural schematic diagram is shown in FIG. 1 ) formed by packing a plurality of modified polyurethane sponge blocks (block diameter is, for example, 2 to 3 cm) into a PP hollow ball (its diameter is, for example, 8 cm) Figure 2 , Figure 4 ), and then fill them into layers on the second supporting mechanism 72, which is more conducive to maintaining the function while reducing the pressure drop of the equipment. The second supporting mechanism 72 can be an ordinary filler supporting plate or other supporting device, as long as it is sufficient to firmly support the second filler and can be ventilated. Preferably, the second supporting mechanism 72 is provided with ventilation holes with a diameter or side length of 5 cm to 7 cm.
[0041] The lower part of the body of the purification device is provided with a water treatment unit, including a water guide plate 28 and a sedimentation tank 8. Specifically, the water guide plate 28 and the sedimentation tank 8 are sequentially arranged from top to bottom in the lower part of the body. The water guide plate is arranged obliquely to receive the sewage falling from the primary filter layer 3 and guide it into the sedimentation tank 8 below. The sewage carries trapped particles and odorous substances dissolved in water, which need to be further treated.
[0042] The sedimentation tank 8 is arranged below the water guide plate 28, and is used to process the sewage intercepted and collected by the water guide plate. Since the concentration of particulate matter in the air of the livestock and poultry house is high, the sedimentation tank 8 is preferably an integrated inclined tube sedimentation tank (i.e., an integrated inclined plate sedimentation tank), which is convenient for timely separation and discharge of particulate matter, reducing the contact time between particulate matter and recycled spray water, reducing the leaching of harmful substances in particulate matter, and reducing the difficulty of subsequent sewage treatment. Specifically, the sedimentation tank 8 includes an inclined plate sedimentation area and a sludge hopper below, and a sludge discharge pipe 9 is provided at the bottom of the sludge hopper. The sewage falling from the primary filter layer 3 is introduced into the sedimentation tank 8 below through the water guide plate. After sedimentation, the particulate matter gathers in the sludge hopper and is discharged through the sludge discharge pipe 9. The sewage passes upward through the inclined plate sedimentation area and is further precipitated and treated to obtain clean water for discharge, which is further treated and reused as appropriate.
[0043] Preferably, the effluent of the sedimentation tank 8 can be led to the clean water tank 12 or the sewage treatment facility through the outlet pipe according to the situation, and a drain valve is provided on the outlet pipe to control the flow direction and flow of the effluent. Specifically, the first outlet of the sedimentation tank 8 is connected to the clean water tank 12 through the first outlet pipe, and the first outlet pipe is provided with a first drain valve 271; the second outlet of the sedimentation tank 8 is connected to the sewage treatment facility through the second outlet pipe, and the second outlet pipe is provided with a second drain valve 272. The sewage treatment facility is placed outside the main body, including a MAP reaction tank (i.e., magnesium ammonium phosphate reaction tank) 20, a PAM (polyacrylamide) filling tank 21, and a maturation tank 22 which are connected in sequence. A stirrer 15 is provided in the MAP reaction tank 20, the PAM filling tank 21, and the maturation tank 22. The MAP reaction tank 20 is connected to a MgCl2 dosing pipe 23 and a Na2HPO4 dosing pipe 24. The MAP reaction tank 20 receives sewage flowing out of the sedimentation tank 8. The sewage and the Mg-containing agent added from the MgCl2 dosing pipe 23 and the Na2HPO4 dosing pipe 24 are mixed. and P-containing reagents to form struvite (i.e., magnesium ammonium phosphate, MgNH4PO4·6H2O, referred to as MAP); the PAM filling tank 21 is connected to a PAM dosing pipe 25, and the sewage flowing out of the MAP reaction tank 20 is mixed with the injected PAM reagent to quickly agglomerate the suspended particles in the sewage, thereby promoting the precipitation of struvite; the maturation tank 22 receives the sewage from the PAM filling tank 21, and the sewage stays here for a certain period of time, which increases the reaction time, allowing the doped sewage to further react fully, and at the same time achieves a certain degree of solid-liquid separation (precipitation). Preferably, the outlet of the maturation tank 22 is connected to a return pipe 19, and the outlet end of the return pipe 19 is connected to the sedimentation tank 8. The sewage treated by the maturation tank 22 is returned to the sedimentation tank 8 for recycling, which can dilute the ammonia nitrogen concentration of the water in the sedimentation tank 8, reduce the conductivity of the water, and meet the circulation spraying requirements, so that the water in the sedimentation tank 8 can be sent to the clean water tank 12 as spray water as much as possible without affecting the performance of the spray circulation pump.
[0044] Preferably, an EC sensor 16, a pH sensor 17 and an ammonia nitrogen sensor 18 are provided in the sedimentation tank 8 to monitor the conductivity, pH and ammonia nitrogen content of the sewage, and to determine whether the effluent of the sedimentation tank 8 is returned to the clean water tank 12 for use as spray water or flows to the sewage treatment facility for further treatment based on the monitoring data. In this embodiment, when the pH is less than 7.2 and EC is less than 20ms / cm, it indicates that the ammonia nitrogen concentration in the sewage is low, and the effluent from the sedimentation tank 8 can directly enter the clean water tank 12 for recycling through the first drain valve 271; when the pH is 7.2-8.5 and EC is less than 20ms / cm, it indicates that there is a certain amount of ammonia nitrogen accumulation in the sewage in the device, but it can still be recycled; when the pH is greater than 8.5 and EC ≥ 20ms / cm, the effluent from the sedimentation tank 8 flows to the sewage treatment facility through the second drain valve 272 for recycling. Further treatment: The ammonia nitrogen concentration in the water is measured by the ammonia nitrogen sensor 18, and the dosage of the additives MgCl2 and Na2HPO4 is determined according to the ammonia nitrogen concentration data. The dosage of Mg and P is controlled by the MgCl2 dosing pipe 23 and the Na2HPO4 dosing pipe 24, so that the molar ratio of N:Mg:P is 1:1:1, and struvite is formed, which can be recycled as a fertilizer resource. As a solid fertilizer, it is also convenient for storage and transportation; the treated water returns to the inclined plate sedimentation tank through the return pipe 19 for precipitation and then recycling. Of course, other sewage treatment processes and facilities can also be used for subsequent sewage treatment recycling.
[0045] In an appropriate scenario, in order to further improve the degree of device integration, the present application also provides a second exemplary structure of an energy-saving livestock and poultry house purification device, see Figure 2, wherein the sewage treatment facility is arranged below the water guide plate 28 in the body and upstream of the sedimentation tank 8. The sewage treatment facility also includes a MAP reaction tank (i.e., magnesium ammonium phosphate reaction tank) 20, a PAM (polyacrylamide) filling tank 21, and a maturation tank 22 which are connected in sequence. A stirrer 15 is arranged in the MAP reaction tank 20, the PAM filling tank 21, and the maturation tank 22. The MAP reaction tank 20 is connected to a MgCl2 dosing pipe 23 and a Na2HPO4 dosing pipe 24. The MAP reaction tank 20 receives the sewage introduced by the water guide plate 28. When necessary, the sewage is mixed with the Mg-containing agent and the P-containing agent added from the MgCl2 dosing pipe 23 and the Na2HPO4 dosing pipe 24. The PAM agent is mixed and reacted to form struvite (i.e., magnesium ammonium phosphate, MgNH4PO4·6H2O, referred to as MAP); the PAM filling tank 21 is connected to the PAM dosing pipe 25, and the sewage flowing out of the MAP reaction tank 20 is mixed with the PAM agent injected at different times to quickly agglomerate the suspended particles in the sewage, promoting the precipitation of struvite and / or other particulate matter; the maturation tank 22 receives the sewage from the PAM filling tank 21, and the sewage stays here for a certain period of time, which increases the reaction time, allowing the doped sewage to further react fully, and at the same time achieves a certain degree of solid-liquid separation (precipitation). The outlet of the maturation tank 22 is connected to the sedimentation tank 8 to further precipitate the sewage. Preferably, an EC sensor 16 is set at the outlet of the sedimentation tank 8 to monitor the conductivity of the sewage outlet; a pH sensor 17 and an ammonia nitrogen sensor 18 are set on the MAP reaction tank 20 to measure the pH and ammonia nitrogen content of the sewage, and whether the effluent of the sedimentation tank 8 is reused as spray water is determined based on the monitoring data.
[0046] In the above embodiment of the present application, when pH is less than 7.2 and EC is less than 20ms / cm, no reagent needs to be added to the MAP reaction tank 20 to remove ammonia nitrogen from the sewage. The sewage passes through the MAP reaction tank 20, the PAM filling tank 21, the maturation tank 22, and then the particles are precipitated in the sedimentation tank 8. The effluent in the sedimentation tank 8 directly enters the clean water tank 12 through the first drain valve 271 for recycling; when pH = 7.2-8.5 and EC < 20ms / cm, it indicates that there is considerable accumulation of ammonia nitrogen in the sewage, and it can continue to be recycled. The ring is used as spray water, and the dosage of added reagents MgCl2 and Na2HPO4 can also be determined according to the ammonia nitrogen concentration data measured by the ammonia nitrogen sensor 18 to form struvite to remove ammonia nitrogen in advance; when pH>8.5, EC≥20ms / cm, it indicates that the ammonia nitrogen content in the sewage is close to saturation, and the effluent in the sedimentation tank 8 can no longer be reused. It needs to be discharged and replaced with new spray water, or the dosage of reagents MgCl2 and Na2HPO4 in the MAP reaction tank 20 needs to be adjusted (increased) to increase the removal of ammonia nitrogen.
[0047] Figure 3The third example structure of the energy-saving livestock and poultry house purification device of the present application is shown, and the only difference between it and the first example structure is that a purification unit is provided in the upper part of the main body, and the purification unit is a traditional horizontal processing layout, that is, it includes a spray part, a primary filter layer 3, and a secondary filter layer 5 arranged in sequence along the horizontal direction, such as from left to right or from right to left; the purification unit is supported by a partition 7.
[0048] The above are some of the preferred structural selections of this application, and the above components can also be other structures. The above preferred structures can be used alone, and can also be used in any combination without conflict, and the effect will be better when used in combination.
[0049] The present application also provides an energy-saving livestock and poultry house purification method, which is implemented by the above-mentioned purification device.
[0050] Preferably, the spray treatment is carried out by an intermittent spraying method.
[0051] Preferably, the intermittent spraying method is: in autumn and winter, first spray water for 8-10 minutes, stop spraying for 50-60 minutes, continue spraying, and repeat the cycle; in spring and summer, first spray water for 8-10 minutes, stop spraying for 20-30 minutes, then continue spraying, and repeat the cycle. The time of spraying and not spraying is generally determined according to the ventilation volume and temperature.
[0052] Preferably, when the pH of the water in the sedimentation tank 8 is less than 7.2 and the EC is less than 20ms / cm, the effluent from the sedimentation tank 8 is transported to the clean water tank 12 for use as spray water; when the pH of the water in the sedimentation tank 8 is greater than 8.5 and the EC is ≥20ms / cm, the effluent from the sedimentation tank 8 is transported to a sewage treatment facility for further treatment.
[0053] Preferably, in the sewage treatment facility, the dosage of the additives MgCl2 and Na2HPO4 is determined according to the ammonia nitrogen concentration of the water, and the molar ratio of N:Mg:P is controlled to be 1:1:1, so as to generate struvite precipitation.
[0054] The present application also provides a new chemical strain for livestock and poultry house purification, which is Bacillus Velez N4, with a deposit number of CGMCC No. 30274. The strain has good deodorization and ammonia removal performance. After forming a film on a polyurethane sponge, its ammonia reduction performance was evaluated, and the removal rate was 95.1%.
[0055] The present application also provides a filter layer filler for purification of livestock and poultry houses, which is made by treating a polyurethane sponge with the Velez subtilis N4 for biofilm formation; preferably, the biofilm formation treatment comprises: firstly streaking the separated, purified and preserved Velez subtilis N4 on an MRS solid culture medium, inverting the separated, purified and preserved Velez subtilis N4 on an MRS solid culture medium, and incubating the culture inverted in a biochemical incubator at 37°C for 24 hours; then using a sterile inoculation loop to pick a single colony of Velez subtilis N4 and inoculate it into an MRS liquid culture medium, and shaking and culturing the culture at 37°C, 150 rpm for 24 hours to obtain a bacterial solution, the absorbance value of the bacterial solution at a wavelength of 600 nm is 1; then immersing the polyurethane sponge in the bacterial solution and letting it stand for 24 hours.
[0056] Example 1 Screening, identification and preservation of Bacillus velez N4.
[0057] (1) Preparation of strains and culture medium:
[0058] The strain originated from feces samples from a pig farm in Chongqing.
[0059] NB medium, YPD medium, and MRS medium were selected to isolate fecal sample strains. The components of each medium are as follows:
[0060] NB medium (g / L): peptone 10.0, beef extract powder 3.0, sodium chloride 5.0, the balance is water, pH 7.2±0.2, 25℃.
[0061] YPD medium (g / L): 20.0% peptone, 20.0% glucose, 10.0% yeast extract powder, the balance is water, pH 6.5±0.2, 25°C.
[0062] MRS medium (g / L): peptone 10.0, beef extract powder 8.0, yeast extract powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1.0, the balance is water, pH 5.7±0.2, 25°C;
[0063] The solid culture medium corresponding to the above three liquid culture media was prepared by adding 16 g / L agar to the above formula.
[0064] (2) Isolation and purification: The strains in the sample obtained in step (1) were isolated and purified. Under a sterile operating environment, 10 g of feces was added to 90 mL of normal saline and mixed, and gradient dilution was performed with sterile distilled water to obtain a bacterial suspension. 100 μL of the bacterial suspension was spread on solid culture media (NB culture media, YPD culture media, and MRS culture media, respectively), inverted in an incubator, and cultured at 37°C for 24 hours. In an ultra-clean workbench, a sterile inoculation loop was used to pick single colonies of different morphologies and streak them in the culture medium. After repeating the streaking on the plate 3 times or more, the purified strains were obtained, and the strains were stored in glycerol at -20°C for later use. A total of 35 strains were isolated and preserved, numbered N1-N35.
[0065] (3) Preliminary screening of ammonia reduction performance of strains: The above strains were inoculated into the corresponding liquid culture medium described in step (1), and cultured at 37°C, 150rpm, and shaking for 24 hours to obtain a bacterial solution. After mixing pig manure and pig sewage in a ratio of 1:1, 50g of water-soaked manure was taken into a 120mm culture dish, and 10mL of bacterial solution was added to the experimental group and 10mL of sterile water was added to the control group. Three parallels were made for each strain. After being placed in a 3L air bag and sealed to exhaust the gas, the air bag was sealed after being inflated uniformly for 30s with a pump. The bag was cultured at a constant temperature of 30°C for 24 hours, and the NH3 concentration was detected. Calculate the NH3 gas removal rate after 24 hours, and select strains with an NH3 removal rate of more than 50% for plotting, such as Figure 5 As shown. Figure 5 It can be seen that strain N4 (isolated and purified from MRS medium) had the highest ammonia removal rate after 24 hours, which was 74.15%. Therefore, strain N4 was selected as the subsequent research object and identified.
[0066] (4) Strain identification:
[0067] (4.1) Morphological identification
[0068] Bacillus Velez N4 was cultured on MRS medium plate at 37℃ for 24h. The colony was as shown in the figure Figure 6 As shown. Figure 6 It can be seen that the colonies of the N4 strain are round or oval, milky white, opaque, with smooth edges and a bulge in the middle. They are sticky and easy to pick up. Gram-positive.
[0069] (4.2) Identification by molecular biological methods
[0070] The genomic DNA of strain N4 was extracted using the TSINGKE kit, and the bacterial universal primers 27F (5'-GAGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTCTTACGAC-3') were used to amplify the 16S rDNA region sequence of the N4 strain. The amplified size was about 1.5 bp, and the 16S rDNA gene was amplified by polymerase chain reaction (PCR). The PCR amplification product was sequenced by the Chongqing Branch of Beijing Qingke Biotechnology Co., Ltd. The sequencing results were spliced using ContigExpress, and the sequencing results were subjected to BLAST similarity analysis with the existing sequences in NCBI (https: / / blast.ncbi.nlm.nih.gov / Blast.cgi). Strain N4 was identified as Bacillus velezensis. The phylogenetic tree was constructed using the Neighbor Joining method of MAGE6 software, as shown in Figure 2. Figure 7
[0071] (5) Preservation of strains
[0072] The inventor named strain N4 Bacillus velezensis N4 and submitted it for preservation. The preservation date is April 7, 2024, the preservation number is CGMCC No.30274, the classification name is: Bacillus velezensis N4, the name of the preservation unit is: General Microbiology Center of China Microbiological Culture Collection Administration (referred to as CGMCC), the address is: No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, Postal Code 100101.
[0073] (6) Strain performance test:
[0074] (6.1) Strain non-toxicity test. Bacillus Velez N4 was inoculated into MRS liquid culture medium and cultured at 37°C, 150rpm, with shaking for 24h. The culture medium was sent to China Inspection and Quarantine Huatongwei International Inspection (Suzhou) Co., Ltd. for oral toxicity median lethal dose LD 50 Detection. Oral toxicity median lethal dose ID 50 The test showed that the acute oral LD50 of the samples in ICR mice 50 >2000mg / kg body weight, according to GB 5085.2-2007 Hazardous Waste Identification Standard Acute Toxicity Screening, oral toxicity median lethal dose LD 50 Test classification criteria for determining LD 50 >500mg / kg body weight, does not have the acute oral toxicity characteristics of hazardous waste.
[0075] (6.2) Identification of ammonia reduction performance:
[0076] 1) Biofilm formation: First, Bacillus Velez N4 was inoculated into MRS liquid culture medium, and cultured at 37°C, 150 rpm, and shaking for 24 hours to obtain a bacterial solution; secondly, the filter element material, i.e., a polyurethane sponge, was immersed in the bacterial solution and placed in an ultra-clean workbench at room temperature for 24 hours to obtain a polyurethane sponge after biofilm formation.
[0077] 2) Test: Put the polyurethane sponge after biofilm formation into a ziplock bag, add an appropriate amount of bacterial solution, put it into a foam box, add ice packs and send it to Guangdong Microbiological Analysis and Testing Center (Address: Building 66, No. 100, Xianlie Middle Road, Guangzhou) for testing and evaluation of its ammonia reduction performance. The test method refers to "QB / T 2761-2006 Method for Determination of Purification Effect of Indoor Air Purification Products". After 24 hours, the blank test chamber concentration value of pollutant ammonia is 2.10mg / m 3 The concentration value of the sample test chamber is 0.103mg / m 3 , the removal rate is 95.1%.
[0078] Example 2
[0079] The livestock and poultry house purification device provided in this preferred embodiment comprises: a vertically erected body; a primary filter layer 3, a spraying part, and a secondary filter layer 5 are sequentially arranged from bottom to top in the upper part of the body; a water guide plate 28 and a sedimentation tank 8 are sequentially arranged from top to bottom in the lower part of the body; an air inlet 26 is arranged on the side of the body, and the air inlet 26 is located below the primary filter layer 3, and an air outlet is arranged on the top of the body. The primary filter layer 3 comprises a first filler and a filler support plate, and the first filler adopts a polypropylene plastic (PP plastic) mesh filler with a porosity of about 97%. The spraying part comprises a spraying water pipe 4 and a plurality of uniformly arranged nozzles 6, the spraying water pipe 4 is connected to an external clean water pool 12, and the nozzle 6 is connected to the spraying water pipe 4, and its opening faces the primary filter layer 3. The secondary filter layer 5 comprises a second filler and a filler support plate, and the second filler adopts a modified polyurethane sponge loaded with modified activated carbon, and the porosity is about 95%. In the lower part of the body, the water guide plate is inclined to receive the sewage falling from the primary filter layer 3 and guide it into the sedimentation tank 8 below; the sedimentation tank 8 is an integrated inclined tube sedimentation tank, including an inclined plate sedimentation area and a sludge hopper below, and a sludge discharge pipe 9 is provided at the bottom of the sludge hopper; the water outlet of the sedimentation tank 8 is connected to the external clean water tank 12 and the external sewage treatment facility respectively. A pH sensor 16, an EC sensor 17 and an ammonia nitrogen sensor 18 are arranged in the sedimentation tank 8 to monitor the pH, conductivity and ammonia nitrogen content of the sewage, and decide whether the effluent of the sedimentation tank 8 is returned to the clean water tank 12 for spraying water or flows to the sewage treatment facility for further treatment according to the monitoring data.
[0080] In the livestock and poultry house purification device provided in the preferred embodiment, the sewage treatment facilities are externally arranged, including a MAP reaction tank 20, a PAM filling tank 21, and a maturation tank 22 which are connected in sequence, wherein the bottom of the MAP reaction tank 20 is connected to the PAM filling tank 21, and the top of the PAM filling tank 21 is connected to the maturation tank 22, which increases the passage path of the wastewater and allows the water flow to flow in a Z-shape, thereby increasing the contact time and reaction time; an agitator 15 is provided in the MAP reaction tank 20, the PAM filling tank 21, and the maturation tank 22, wherein the MAP reaction tank 20 is connected to a MgCl2 dosing pipe 23 and a Na2HPO4 dosing pipe 24; the PAM filling tank 21 is connected to a PAM dosing pipe 25; and the outlet of the maturation tank 22 is connected to the sedimentation tank 8 through a reflux pipe 19.
[0081] A spray circulation pump 13 is arranged at the bottom of the clean water tank 12, and the spray water pipe 4 is connected to the spray circulation pump 13 through a pipeline, and a flow meter 11 and a circulation pump timing switch controller 10 are arranged on the pipeline; the clean water tank 12 is also connected to the outlet pipe of the sedimentation tank 8, and when the outlet water quality of the sedimentation tank 8 meets specific requirements, it can flow back to the clean water tank 12 and be circulated for use as spray water.
[0082] The livestock and poultry house purification method provided in this preferred embodiment is implemented by the livestock and poultry house purification device described above. The main process flow of purification is as follows: mainly using intermittent spraying method; in autumn and winter, first spray water for 8-10 minutes, stop spraying for 50-60 minutes, continue spraying, and repeat the cycle; in spring and summer, first spray water for 8-10 minutes, stop spraying for 20-30 minutes, continue spraying, and repeat the cycle to achieve the purpose of reducing water and electricity consumption.
[0083] The second filler uses deodorizing microorganisms to form biofilms, and the specific biofilm forming process is as follows: in a clean bench, the isolated, purified and preserved Velez Bacillus N4 is streaked on the MRS solid culture medium, and then placed upside down in a biochemical incubator at 37°C for 24 hours; then, in the clean bench, a single colony of Velez Bacillus N4 is picked up with a sterile inoculation loop and inoculated into the MRS liquid culture medium, and the culture is shaken at 37°C, 150rpm for 24 hours to obtain a bacterial solution, and the absorbance value of the bacterial solution at a wavelength of 600nm is detected to be 1. A modified polyurethane sponge with a size of 2 to 3 cm is immersed in the above bacterial solution, left to stand for 24 hours, and then loaded into an 8 cm PP hollow ball.
[0084] The specific purification process is as follows:
[0085] Under the action of the negative pressure fan, the odor 1 enters the purification device from the air inlet 26, flows upward, and passes through the primary filter layer 3. There are spray water pipes 4 and nozzles 6 above the primary filter layer 3. The spray water is sprayed toward the primary filter layer 3 and contacts with the countercurrent odor 1. On the one hand, the spray water can fully contact with the odor, so that some soluble odors such as ammonia and soluble VOCs are dissolved in the water. On the other hand, it can achieve the purpose of dust removal.
[0086] The odor with water mist continues to flow upward through the secondary filter layer 5. The secondary filter layer 5 is filled with modified polyurethane sponge, with a porosity of about 95% and a height of 0.3m. On the one hand, the modified polyurethane sponge uses a polyurethane sponge loaded with activated carbon to increase the adsorption of hydrogen sulfide, ammonia and VOCs in the odor of livestock and poultry houses. In addition, the polyurethane sponge has a biofilm deodorizing microorganism Bacillus Velez N4 to further transform the adsorbed odorous substances. On the other hand, the polyurethane sponge has strong water absorption, and its water absorption capacity can be as high as 300%. It can absorb the water mist brought from the primary filter layer 3 and increase the humidity of the filter material of the secondary filter layer 5, so that during the period of stopping spraying, the odor passing through can still be transformed from the gas phase to the water phase, and further adsorbed and transformed by the secondary filter layer 5. It is particularly suitable for the intermittent spraying process of the present application, and plays a role in reducing water and electricity consumption.
[0087] In addition, due to the high concentration of particulate matter in the air of livestock and poultry houses, the sedimentation tank 8 arranged below the purification device is an integrated inclined plate sedimentation tank (also known as an inclined tube sedimentation tank), which can separate the particulate matter more quickly and discharge it in time through the mud discharge pipe 9 below, thereby reducing the contact time between the particulate matter and the recovered spray water, reducing the leaching of pollutants in the particulate matter, increasing the number of water recycling times, and improving water utilization. EC sensor 16, pH sensor 17 and ammonia nitrogen sensor 18 are set up to control the direction of the effluent from the sedimentation tank according to their detection data. When pH is less than 7.2 and EC is less than 20ms / cm, the water in the inclined plate sedimentation tank enters the clean water tank 12 for recycling through the first drain valve 271; when pH is greater than 8.5 and EC is greater than or equal to 20ms / cm, the water in the inclined plate sedimentation tank enters the MAP reaction tank 20 through the second drain valve 272. The dosage of Mg and P is determined according to the ammonia nitrogen concentration measured by the ammonia nitrogen sensor 18, and the reagents are added through the MgCl2 dosing pipe 23 and the Na2HPO4 dosing pipe 24, so that the molar ratio of N:Mg:P is 1:1:1, and struvite is formed, which can be recycled as fertilizer resources. The water treated in the maturation tank 22 is returned to the inclined plate sedimentation tank through the return pipe 19 for recycling after precipitation.
[0088] The purification device of this preferred embodiment is applied to a fattening pig house in a pig farm in Rongchang District, Chongqing. The pig house is 60m long and 9m wide, with 21 pens on each side, a total of 42 pens, see Figure 8 The purification device of the preferred embodiment is used at the negative pressure fan at one end of the pig house ( Figure 8 (not shown), the length, width and height dimensions of the first filter material of the primary filter layer 3 are 13.2m*2.4m*0.2m, and the length, width and height dimensions of the second filter material of the secondary filter layer 5 are 13.2m*2.4m*0.3m.
[0089] Use a 1.5kW spray pump to spray intermittently, spray for 10 minutes each time, stop spraying for 50 minutes, and the spray density is 0.21m 3 / (㎡˙h), repeat this process 24 times a day. When only one variable frequency fan is turned on, the average wind speed is 0.8m / s and the average ventilation volume is 3200m 3 After stable operation under the condition of 1000 rpm / h, 3 gas samples were collected from the inlet and outlet of the device during the spraying and non-spraying periods on the 1st, 4th, 7th, 14th and 30th days, and the water meter and electricity meter readings were recorded. The average concentrations of ammonia inlet and outlet were 11.3ppm and 2.2ppm, respectively, with an average removal rate of 72.45%; the average daily fresh water consumption was 0.04m 3 / d, with an average daily power consumption of 6.47kWh / d. Compared with the daily power consumption of 36kWh / d of the continuous spray purification system with a 1.5kW spray pump, the power consumption can be saved by 82.0%, and the daily fresh water consumption is 0.70m 3 / d, the daily water consumption can be saved by 94.2%, which greatly reduces the energy and water consumption of the equipment.
[0090] The applicant commissioned Chongqing Baishijia Environmental Protection Technology Co., Ltd. to test the odor concentration. The test was based on HJ1262-2022 "Three-point comparison odor bag method for determination of odor in ambient air and exhaust gas". The average odor value (dimensionless) before treatment by the equipment was measured to be 332, and the odor values after treatment by the equipment were all <10.
[0091] Finally, it should be noted that in the present disclosure, "A and / or B" should be interpreted as any one of the following three parallel situations: A; B; A and B.
[0092] If any, in this application, relational terms such as left and right, first and second, etc. are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprise", "include" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the statement "comprise a ..." do not exclude the presence of other identical elements in the process, method, article or device including the elements.
[0093] Although the present application has been disclosed above through the description of the specific embodiments of the present application, it should be understood that those skilled in the art can design various modifications, improvements or equivalents to the present application within the spirit and scope of the attached scheme. These modifications, improvements or equivalents should also be considered to be included in the scope of protection claimed in the present application.
Claims
1. An energy-saving livestock and poultry house purification device, characterized in that: include: A body erected vertically; The purification unit is arranged in the upper part of the body, and includes a primary filter layer, a spray element, and a secondary filter layer arranged in sequence from bottom to top, or includes a spray element, a primary filter layer, and a secondary filter layer arranged in sequence along the horizontal direction; A water treatment unit is arranged in the lower part of the body, and includes a water guide plate and a sedimentation tank arranged in sequence from top to bottom; An air inlet is provided on the side of the body, the air inlet is located below or in front of the primary filter layer and above the water guide plate, and an air outlet is provided on the top or side of the body; The secondary filtration layer includes a second filler and a second supporting structure for supporting the second filler; the second filler is a modified polyurethane sponge with a porosity of 95% to 97%, and the modified polyurethane sponge is a modified polyurethane sponge loaded with modified activated carbon and / or zeolite powder; the second filler is formed with deodorizing microorganisms, and the deodorizing microorganisms are Bacillus Velez N4, and the preservation number is CGMCC No.30274.
2. The energy-saving livestock and poultry house purification device according to claim 1 is characterized in that: The primary filter layer includes a first filler and a first supporting structure for supporting the first filler; the first filler is at least one selected from polypropylene plastic, polyethylene plastic, volcanic rock, and ceramics, and has a porosity of 95%-97%.
3. The energy-saving livestock and poultry house purification device according to claim 1 or 2, characterized in that: Also includes: A clean water pool is connected to the spraying member and is used to provide spraying water. The clean water pool is also connected to the water outlet of the sedimentation tank.
4. The energy-saving livestock and poultry house purification device according to claim 3 is characterized in that: The spraying member comprises a spraying water pipe and a nozzle. The nozzle is connected to the spraying water pipe and its opening faces the primary filter layer.
5. The energy-saving livestock and poultry house purification device according to any one of claims 1-2 and 4, wherein OA1 The characteristic is that the second filler comprises a modified polyurethane sponge filler sphere formed by packing a plurality of modified polyurethane sponge blocks into a hollow sphere.
6. The energy-saving livestock and poultry house purification device according to claim 5 is characterized in that: The block diameter of the modified polyurethane sponge block is 2-3 cm, and the hollow ball is made of PP material and has a diameter of 8 cm.
7. The energy-saving livestock and poultry house purification device according to any one of claims 1-2, 4, and 6, characterized in that: In the water treatment unit, the water guide plate is arranged obliquely to receive the sewage falling from the primary filter layer and guide it into the sedimentation tank, and the sedimentation tank is an integrated inclined tube sedimentation tank.
8. The energy-saving livestock and poultry house purification device according to claim 7 is characterized in that: A pH sensor, an EC sensor and an ammonia nitrogen sensor are arranged in the sedimentation tank to monitor the pH, conductivity and ammonia nitrogen content of the sewage. According to the monitoring data, it is determined whether the effluent of the sedimentation tank is directly used as spray water or flows to the sewage treatment facility for further treatment.
9. The energy-saving livestock and poultry house purification device according to claim 8, characterized in that: The water treatment unit also includes a sewage treatment facility, which is arranged outside the main body and connected to the water outlet of the sedimentation tank; the sewage treatment facility includes a MAP reaction tank, a PAM filling tank, and a maturation tank that are connected in sequence, and the MAP reaction tank, the PAM filling tank, and the maturation tank are all equipped with a stirrer, wherein the MAP reaction tank is connected to a MgCl2 dosing pipe and a Na2HPO4 dosing pipe; the PAM filling tank is connected to a PAM dosing pipe.
10. The energy-saving livestock and poultry house purification device according to claim 9, characterized in that: The water outlet of the maturation tank is connected with a return pipe, and the water outlet end of the return pipe is connected to the sedimentation tank.
11. The energy-saving livestock and poultry house purification device according to any one of claims 1-2, 4 and 6, characterized in that: The water treatment unit also includes a sewage treatment facility, which is arranged in the main body, below the water guide plate, and upstream of the sedimentation tank; the water guide plate is inclined to receive the sewage falling from the primary filter layer and guide it into the sewage treatment facility; the sedimentation tank is an integrated inclined tube sedimentation tank.
12. The energy-saving livestock and poultry house purification device according to claim 11, characterized in that: The sewage treatment facility comprises a MAP reaction tank, a PAM filling tank and a maturation tank which are connected in sequence. Agitators are provided in the MAP reaction tank, the PAM filling tank and the maturation tank. The MAP reaction tank is connected with a MgCl2 dosing pipe and a Na2HPO4 dosing pipe; the PAM filling tank is connected with a PAM dosing pipe; and the water outlet of the maturation tank is connected with the sedimentation tank.
13. The energy-saving livestock and poultry house purification device according to claim 12, characterized in that: An EC sensor is arranged at the outlet of the sedimentation tank to monitor the conductivity of the outlet water; a pH sensor and an ammonia nitrogen sensor are arranged on the MAP reaction tank to measure the pH and ammonia nitrogen content of the sewage.
14. An energy-saving livestock and poultry house purification method, characterized in that: The purification device according to any one of claims 1 to 13 is implemented by adopting an intermittent spraying method for spraying treatment.
15. The energy-saving livestock and poultry house purification method according to claim 14, characterized in that: The intermittent spraying method is: in autumn and winter, first spray water for 8-10 minutes, stop spraying for 50-60 minutes, continue spraying, and repeat the cycle; in spring and summer, first spray water for 8-10 minutes, stop spraying for 20-30 minutes, then continue spraying, and repeat the cycle.
16. The energy-saving livestock and poultry house purification method according to claim 14 or 15, characterized in that: When the pH of the water in the sedimentation tank is less than 7.2 or the EC is less than 20ms / cm, it is recycled and used as spray water; when the pH of the water in the sedimentation tank is greater than 8.5 and the EC is greater than or equal to 20ms / cm, it is transported to a sewage treatment facility for further treatment.
17. The energy-saving livestock and poultry house purification method according to claim 16, characterized in that: In the sewage treatment facility, the dosage of the additives MgCl2 and Na2HPO4 is determined according to the ammonia nitrogen concentration of the water, and the molar ratio of N:Mg:P is controlled to be 1:1:1 to form struvite precipitation.
18. A new strain for purification of livestock and poultry houses, the new strain is Bacillus Velez N4, and its deposit number is CGMCC No.30274.
19. A filter layer filler for purification of livestock and poultry breeding houses, characterized in that: The filter layer filler is made by forming a polyurethane sponge with the Bacillus Velez N4 as claimed in claim 18.
20. The filter layer filler for purifying livestock and poultry breeding houses according to claim 19, characterized in that: The biofilm forming method comprises: firstly, streaking the preserved Velez Bacillus N4 on an MRS solid culture medium, and placing the culture medium upside down in a biochemical culture box at 37°C for 24 hours; then, picking a single colony of the Velez Bacillus N4 with a sterile inoculation loop and inoculating it into an MRS liquid culture medium, and shaking and culturing the culture medium at 37°C and 150 rpm for 24 hours to obtain a bacterial solution, wherein the absorbance value of the bacterial solution at a wavelength of 600 nm is 1; and finally, immersing a polyurethane sponge in the bacterial solution and allowing the culture medium to stand for 24 hours.
Citation Information
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