Skin and mucous membrane disinfectant and preparation method thereof

Abstract

The invention discloses a skin mucosal disinfectant and a preparation method thereof, belonging to the technical field of disinfection, wherein the disinfectant comprises, by weight percentage, 0.15%-0.20% of povidone iodine, 0.05%-0.10% of cyclodextrin-iodine complex, 0.18%-0.22% of composite quaternary ammonium salt, 1%-2% of stabilizer, and the balance is purified water. The skin mucosal disinfectant provided by the invention can effectively kill Staphylococcus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, poliovirus, rabies virus CVS-11 strain, etc. in a short time, and meets strict disinfection requirements, and has the advantages of broad-spectrum and efficient sterilization, no skin irritation, good storage stability, easy cleaning without yellowing, and no unpleasant odor.

Description

Technical Field

[0001] The present invention relates to the technical field of disinfection, and in particular to a skin and mucous membrane disinfectant and a preparation method thereof. Background Art

[0002] Skin and mucous membranes are the first line of defense against infection, helping the body resist external pathogenic factors. Therefore, killing or inhibiting microorganisms on the surface of skin and mucous membranes is important for maintaining human health. Disinfectant is a liquid disinfectant that is often used to disinfect skin, wounds and mucous membranes because of its ease of use and good disinfection and sterilization effects. Current disinfectant products include halogen disinfectants, iodine disinfectants, peroxide disinfectants, alcohol disinfectants, quaternary ammonium disinfectants, compound quaternary ammonium disinfectants, etc.

[0003] CN114027321A discloses an iodine disinfectant and a preparation method thereof, wherein the iodine disinfectant comprises the following components in weight: 0.1-3 parts of iodine, 5-20 parts of organic acid, 0.05-3 parts of potassium iodide, 1-15 parts of solvent, 1-20 parts of disinfection enhancer, and the rest is purified water. The invention combines organic acid and iodine to increase the acidity of the iodine disinfectant, increase the free iodine, and improve the bactericidal effect. Although the disinfectant provided by the invention has the characteristics of broad-spectrum and high-efficiency bactericidal effect and low toxicity, the iodine ion is extremely active during storage and has a very strong oxidizing power, so it is an unstable substance that reduces the effective content and reduces the disinfection effect.

[0004] CN104434994A discloses a skin and mucous membrane disinfectant, which includes the following components in the following weight parts: 5-10 parts of chitosan quaternary ammonium salt, 3-5 parts of alkyl dimethyl benzyl ammonium chloride, 5-10 parts of sodium sulfate of nonylphenol polyoxyethylene ether, 5-10 parts of ethanol, 3-5 parts of beeswax, 0.15%-0.20% of povidone iodine, 3-5 parts of surfactant; 1-3 parts of urea, and 30-40 parts of deionized water. The skin and mucous membrane disinfectant provided by the invention has no irritating odor, low toxicity, weak irritation to the skin and eyes, and is suitable for the disinfection of skin and mucous membranes, but the stability of povidone iodine is low, which will affect the antibacterial effect of the disinfectant. Summary of the invention

[0005] In view of the above-mentioned defects of the prior art, the present invention provides a skin and mucous membrane disinfectant, which can effectively kill Staphylococcus aureus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, polio virus, rabies virus CVS-11 strain, etc. in a short time, meet strict disinfection requirements, and have the advantages of broad-spectrum and high-efficiency sterilization, no skin irritation, good storage stability, easy cleaning without yellowing, no unpleasant odor, etc. The preparation method of the disinfectant of the present invention is simple, stable and reliable, has a short preparation cycle, has no special requirements for equipment, and is very suitable for industrial production.

[0006] The present invention provides a method for preparing a skin and mucous membrane disinfectant, comprising the following steps:

[0007] Povidone iodine, cyclodextrin-iodine complex, and then add povidone iodine and cyclodextrin-iodine complex to purified water, mix and stir for 10-15 minutes to obtain a mixed solution; add composite quaternary ammonium salt and erythritol, then add composite quaternary ammonium salt to the mixed solution, and then add erythritol and mix and stir for 10-20 minutes to obtain a skin and mucous membrane disinfectant. Preferably, by weight percentage, each raw material component includes: povidone iodine 0.15%-0.20%, cyclodextrin-iodine complex 0.05%-0.10%, composite quaternary ammonium salt 0.18%-0.22%, stabilizer 1%-2%, and the balance is purified water.

[0008] Preferably, the method for preparing the cyclodextrin-iodine complex comprises the following steps, measured by weight:

[0009] 1.8-2.2 parts of a cyclodextrin derivative are mixed with 0.3-0.5 parts of iodine powder to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 55-65°C for heat treatment for 6-8 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 23-27°C for 10-12 hours to obtain a cyclodextrin-iodine complex.

[0010] Further preferably, the cyclodextrin derivative is selected from one of amino-α-cyclodextrin, mono-6-O-amino-β-cyclodextrin, thiol-β-cyclodextrin and glucosyl-β-cyclodextrin.

[0011] The inventors found that the cyclodextrin derivatives amino-α-cyclodextrin, mono-6-O-amino-β-cyclodextrin, thiol-β-cyclodextrin, and glucosyl-β-cyclodextrin were mixed with iodine powder respectively, and after sealed heat treatment, the hydrophobic central cavity in the structure of the cyclodextrin derivative can form an inclusion complex by inclusion molecular iodine, that is, a cyclodextrin-iodine complex. The formation of the cyclodextrin-iodine complex improves the stability of iodine, reduces the volatilization loss of iodine, and is conducive to maintaining the concentration of iodine during storage. At the same time, the hydrophilic surface outside the cyclodextrin-iodine complex can improve the solubility of the complex in water, which is conducive to increasing the uniform dispersibility of iodine in aqueous solution. The inventors have found that compared with mono-6-O-amino-β-cyclodextrin, thiol-β-cyclodextrin, and glucosyl-β-cyclodextrin, amino-α-cyclodextrin contains multiple amino groups in its structure, which can increase the interaction with iodine molecules during the formation of the complex, thereby forming a more stable complex, and the polarity of the amino group is conducive to increasing the water solubility of the entire complex, thereby more effectively dispersing iodine in an aqueous environment, avoiding the aggregation and precipitation of iodine, improving the stability of the solution, and increasing the antibacterial activity of iodine. In addition, amino-α-cyclodextrin forms a complex with iodine and is introduced into the disinfectant, which can bring more free amino groups, increase the binding force of the disinfectant with lipopolysaccharide substances in the bacterial structure during the disinfection process, thereby affecting its physiological function and ultimately inhibiting its reproduction, thereby improving the bactericidal effect of the disinfectant.

[0012] Preferably, the composite quaternary ammonium salt is selected from at least three of didodecyldimethylammonium bromide, didodecyldimethylammonium chloride, dioctyldimethylammonium chloride, didecyldimethylammonium bromide, didecyldimethylammonium chloride, dodecyldimethylbenzylammonium chloride, dodecyldimethylbenzylammonium bromide, dodecyltrimethylammonium chloride, dodecyldimethyl-2-phenoxyethylammonium bromide, hexadecyldimethylbenzylammonium bromide, and octadecyldimethylbenzylammonium chloride.

[0013] Preferably, the composite quaternary ammonium salt is a mixture of didodecyldimethylammonium bromide, didecyldimethylammonium bromide and dodecyldimethyl-2-phenoxyethylammonium bromide in a weight ratio of 1:1-2:1-2.

[0014] Preferably, the stabilizer is selected from at least one of erythritol, xylitol and mannitol.

[0015] The present invention also provides a skin and mucous membrane disinfectant, which is prepared by the above-mentioned preparation method.

[0016] Beneficial effects of the present invention:

[0017] 1. The present invention uses the cyclodextrin-iodine complex formed by amino-α-cyclodextrin and iodine in coordination with povidone iodine, which not only increases the content of effective iodine in the disinfectant, but also stabilizes the iodine molecules in povidone iodine, improves the stability of povidone iodine in the solution, and makes the disinfectant still have excellent sterilization and disinfection effects during long-term storage. At the same time, compound quaternary ammonium salt and erythritol are added to the cyclodextrin-iodine complex formed by amino-α-cyclodextrin and iodine, and povidone iodine is mixed. Erythritol may interact with iodine in the disinfectant through hydrogen bonding, further stabilize iodine molecules, and reduce its volatilization during storage. At the same time, erythritol has high stability and is not easily affected by environmental factors, which is conducive to maintaining the stability of the entire disinfectant system and extending the storage time of the disinfectant. The cyclodextrin-iodine complex formed by the compound quaternary ammonium salt, erythritol, amino-α-cyclodextrin and iodine synergistically kills bacteria, expands the antibacterial spectrum, and enhances the sterilization effect of the disinfectant. In addition, the synergistic use of erythritol and cyclodextrin-iodine complex can act as a protective agent to reduce the irritation that may be caused by direct contact of high concentrations of iodine with the skin and mucous membranes, thereby improving the safety of the entire solution.

[0018] 2. The present invention uses the cyclodextrin-iodine complex formed by amino-α-cyclodextrin and iodine in coordination with erythritol, povidone iodine, and composite quaternary ammonium salt to prolong the time of antibacterial substances in the disinfectant on the skin surface, continuously exert a bactericidal effect, and can maintain a high efficiency of up to 48 hours of bactericidal ability under normal conditions, significantly reducing the infection rate of wounds, which is conducive to reducing complications caused by wound infection, reducing medical expenses, and reducing the pain of patients. When the disinfectant acts on the skin, the cyclodextrin-iodine complex formed by amino-α-cyclodextrin and iodine adheres to the skin, and its hydrophilic outer surface and hydrophobic inner cavity help it interact with lipids and water molecules on the skin surface, thereby promoting the diffusion and penetration of iodine molecules in the skin, making it deeper into skin wrinkles and cracks, expanding the antibacterial range and prolonging the action time. At the same time, because erythritol has low hygroscopicity and good water solubility, it may help more cyclodextrin-iodine complexes to adhere to the skin surface, and help prolong its residence time on the skin surface, thereby further increasing the antibacterial duration of the disinfectant and achieving long-term antibacterial. DETAILED DESCRIPTION

[0019] Parameters for specific chemical substances used, sources.

[0020] Povidone iodine, purity: 99%, produced by Suzhou Yaco Technology Co., Ltd., product number: J0426.

[0021] Example 1

[0022] A method for preparing a skin and mucous membrane disinfectant comprises the following steps:

[0023] According to the weight ratio of the proposed formula, 0.16% povidone iodine and 0.06% cyclodextrin-iodine complex are weighed, and then the povidone iodine and cyclodextrin-iodine complex are added to purified water in turn, and the mixture is stirred for 12 minutes to obtain a mixed solution; according to the weight ratio of the proposed formula, 0.20% complex quaternary ammonium salt and 1.5% erythritol are weighed, and then the complex quaternary ammonium salt is added to the mixed solution, and then erythritol is added and stirred for 15 minutes to obtain a skin and mucous membrane disinfectant.

[0024] The composite quaternary ammonium salt is prepared by mixing didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium bromide and dodecyl dimethyl-2-phenoxyethyl ammonium bromide in a weight ratio of 1:2:2.

[0025] The preparation method of the cyclodextrin-iodine complex comprises the following steps:

[0026] 2 parts by weight of amino-α-cyclodextrin and 0.45 parts by weight of iodine powder are mixed to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 63°C for heat treatment for 7 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 25°C for 11 hours to obtain a cyclodextrin-iodine complex.

[0027] Example 2

[0028] A method for preparing a skin and mucous membrane disinfectant, which is different from Example 1 in that the method for preparing the cyclodextrin-iodine complex comprises the following steps:

[0029] 2 parts by weight of mono-6-O-amino-β-cyclodextrin and 0.45 parts by weight of iodine powder are mixed to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 63° C. for heat treatment for 7 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 25° C. for 11 hours to obtain a cyclodextrin-iodine complex.

[0030] Example 3

[0031] A method for preparing a skin and mucous membrane disinfectant, which is different from Example 1 in that the method for preparing the cyclodextrin-iodine complex comprises the following steps:

[0032] 2 parts by weight of mercapto-β-cyclodextrin and 0.45 parts by weight of iodine powder are mixed to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 63° C. for heat treatment for 7 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 25° C. for 11 hours to obtain a cyclodextrin-iodine complex.

[0033] Example 4

[0034] A method for preparing a skin and mucous membrane disinfectant, which is different from Example 1 in that the method for preparing the cyclodextrin-iodine complex comprises the following steps:

[0035] 2 parts by weight of glucosyl-β-cyclodextrin and 0.45 parts by weight of iodine powder are mixed to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 63°C for heat treatment for 7 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 25°C for 11 hours to obtain a cyclodextrin-iodine complex.

[0036] Example 5

[0037] A method for preparing a skin and mucous membrane disinfectant, which differs from Example 1 in that 1.5% erythritol is replaced by 1.5% xylitol.

[0038] Example 6

[0039] A method for preparing a skin and mucous membrane disinfectant, which differs from Example 1 in that 1.5% erythritol is replaced by 1.5% mannitol.

[0040] Comparative Example 1

[0041] A method for preparing a skin and mucous membrane disinfectant comprises the following steps:

[0042] According to the weight ratio of the proposed formula, 0.16% povidone iodine is weighed, and then the povidone iodine is added to purified water, and the mixture is stirred for 12 minutes to obtain a mixed solution; according to the weight ratio of the proposed formula, 0.20% of the complex quaternary ammonium salt and 1.5% of erythritol are weighed, and then the complex quaternary ammonium salt is added to the mixed solution, and then erythritol is added and stirred for 15 minutes to obtain a skin and mucous membrane disinfectant.

[0043] The composite quaternary ammonium salt is prepared by mixing didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium bromide and dodecyl dimethyl-2-phenoxyethyl ammonium bromide in a weight ratio of 1:2:2.

[0044] Comparative Example 2

[0045] A method for preparing a skin and mucous membrane disinfectant comprises the following steps:

[0046] According to the weight ratio of the proposed formula, 0.16% povidone iodine and 0.06% cyclodextrin-iodine complex are weighed, and then the povidone iodine and cyclodextrin-iodine complex are added to purified water in turn, and the mixture is stirred for 12 minutes to obtain a mixed solution; according to the weight ratio of the proposed formula, 0.20% complex quaternary ammonium salt is weighed, and then the complex quaternary ammonium salt is added to the mixed solution, and the mixture is stirred for 15 minutes to obtain a skin and mucous membrane disinfectant.

[0047] The composite quaternary ammonium salt is prepared by mixing didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium bromide and dodecyl dimethyl-2-phenoxyethyl ammonium bromide in a weight ratio of 1:2:2.

[0048] The preparation method of the cyclodextrin-iodine complex is consistent with that in Example 1.

[0049] Comparative Example 3

[0050] A method for preparing a skin and mucous membrane disinfectant comprises the following steps:

[0051] According to the weight ratio of the proposed formula, 0.16% povidone iodine and 0.06% cyclodextrin-iodine complex are weighed, and then the povidone iodine and cyclodextrin-iodine complex are added to purified water in turn, and the mixture is stirred for 12 minutes to obtain a mixed solution; according to the weight ratio of the proposed formula, 0.20% complex quaternary ammonium salt and 1.5% erythritol are weighed, and then the complex quaternary ammonium salt is added to the mixed solution, and then erythritol is added and stirred for 15 minutes to obtain a skin and mucous membrane disinfectant.

[0052] The composite quaternary ammonium salt is prepared by mixing didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium bromide and dodecyl dimethyl-2-phenoxyethyl ammonium bromide in a weight ratio of 1:2:2.

[0053] The preparation method of the cyclodextrin-iodine complex comprises the following steps:

[0054] 2 parts by weight of α-cyclodextrin and 0.45 parts by weight of iodine powder are mixed to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 63°C for heat treatment for 7 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 25°C for 11 hours to obtain a cyclodextrin-iodine complex.

[0055] Comparative Example 4

[0056] A method for preparing a skin and mucous membrane disinfectant comprises the following steps:

[0057] According to the weight ratio of the proposed formula, 0.16% povidone iodine and 0.06% cyclodextrin-iodine complex are weighed, and then the povidone iodine and cyclodextrin-iodine complex are added to purified water in turn, and the mixture is stirred for 12 minutes to obtain a mixed solution; according to the weight ratio of the proposed formula, 0.20% complex quaternary ammonium salt and 1.5% erythritol are weighed, and then the complex quaternary ammonium salt is added to the mixed solution, and then erythritol is added and stirred for 15 minutes to obtain a skin and mucous membrane disinfectant.

[0058] The composite quaternary ammonium salt is prepared by mixing didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium bromide and dodecyl dimethyl-2-phenoxyethyl ammonium bromide in a weight ratio of 1:2:2.

[0059] The preparation method of the cyclodextrin-iodine complex comprises the following steps:

[0060] 2 parts by weight of (2-hydroxypropyl)-α-cyclodextrin and 0.45 parts by weight of iodine powder are mixed to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene lid, and then placed in an oven at 63°C for heat treatment for 7 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 25°C for 11 hours to obtain a cyclodextrin-iodine complex.

[0061] Test Example 1

[0062] Sterilization effect detection

[0063] The artificial skin was used to simulate human skin. According to the method 2.1.1.2.4 in the Technical Specifications for Disinfectants (2002 Edition) , the artificial skin was used as a carrier to prepare Staphylococcus aureus (ATCC 6538) bacterial tablets, Escherichia coli (8099) bacterial tablets, and Pseudomonas aeruginosa (ATCC 15442) bacterial tablets, respectively. Then, according to the method 2.1.1.7.5 in the Technical Specifications for Disinfectants (2002 Edition) , the carrier immersion quantitative sterilization test was used to detect the sterilization effect. The sterilization rates of the disinfectants of Examples 1-6 and Comparative Examples 1-4 on Staphylococcus aureus (ATCC 6538), Escherichia coli (8099), and Pseudomonas aeruginosa (ATCC 15442) were calculated for 0.5 min. The test results were averaged. The specific data are shown in Table 1.

[0064] Persistent antibacterial effect detection

[0065] In the same manner as above, Staphylococcus aureus (ATCC 6538) bacterial slices, Escherichia coli (8099) bacterial slices, and Pseudomonas aeruginosa (ATCC 15442) bacterial slices were prepared, and the initial colony counts on the bacterial slices were recorded. Then, an equal amount of the disinfectant of Examples 1-6 and Comparative Examples 1-4 was dripped on each bacterial slice, and then the colony counts on the bacterial slices were recorded after 48 hours, and the antibacterial rate was calculated. The antibacterial rate calculation formula is as follows:

[0066] Antibacterial rate (%) = (A0-A) / A0×100%

[0067] Where A0 is the initial colony count, and A is the colony count after 48 h.

[0068] The test results are averaged, and the specific data are shown in Table 1.

[0069] Table 1 Sterilization rate and antibacterial rate of each disinfectant

[0070]

[0071] As shown in Table 1, the disinfectants prepared in Examples 1-6 and Comparative Examples 1-4 have a high sterilization rate for Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa within 0.5 min, and have excellent bactericidal properties.

[0072] By comparing the 48h antibacterial rates of Examples 1-6 and Comparative Examples 1-4, it was found that the antibacterial rates of Examples 1-6 and Comparative Examples 2-4 containing cyclodextrin-iodine complexes were significantly higher than those of Comparative Example 1 without cyclodextrin-iodine complexes; by comparing Examples 1-6 and Comparative Examples 2-4, it was found that the antibacterial rates of Example 1 in which amino-α-cyclodextrin was added to form a cyclodextrin-iodine complex were higher than those of Example 2 in which mono-6-O-amino-β-cyclodextrin was added to form a cyclodextrin-iodine complex, and Example 3 in which thiol-β-cyclodextrin was added to form a cyclodextrin-iodine complex. Example 3, Example 4 in which glucosyl-β-cyclodextrin is added to form a cyclodextrin-iodine complex, Comparative Example 3 in which α-cyclodextrin is added to form a cyclodextrin-iodine complex, and Comparative Example 4 in which (2-hydroxypropyl)-α-cyclodextrin is added to form a cyclodextrin-iodine complex; it is found that the antibacterial rates of Example 1 using erythritol as a stabilizer, Example 5 using xylitol as a stabilizer, and Example 6 using mannitol and xylitol as a stabilizer are higher than those of Comparative Example 2 in which no stabilizer is added, and the antibacterial rate of Example 1 is higher than that of Examples 5-6. In summary, it is shown that the addition of cyclodextrin-iodine complex and stabilizer to the disinfectant is beneficial to improving the 48h antibacterial rate of the disinfectant and achieving long-term antibacterial effect. Among them, the cyclodextrin-iodine complex formed by adding amino-α-cyclodextrin to the disinfectant and erythritol are used in conjunction with povidone iodine and composite quaternary ammonium salt to improve the antibacterial rate.

[0073] Test Example 2

[0074] Stability test

[0075] Accelerated test: Take 10 mL of the disinfectant prepared by Examples 1-6 and Comparative Examples 1-4 of the present invention, respectively, and divide them into Example 1-6 and Comparative Example 1-4 groups, then test the initial effective iodine content in each group of disinfectants, and then store them in a 54°C electric thermostat for 5d, 10d and 15d, respectively, take samples and cool them to room temperature, and test the effective iodine content of each group of disinfectants after storage in the electric thermostat. Among them, the effective iodine content in the disinfectant is measured three times for each group, and the effective iodine loss rate is calculated based on the initial effective iodine content and the effective iodine content after storage. The calculation formula of the effective iodine loss rate is as follows:

[0076] Effective iodine loss rate (%) = (B0-B x ) / B0×100%

[0077] In the formula, B0 is the initial effective iodine content, B x It is the effective iodine content on the Xth day of storage in the electric incubator.

[0078] The test results are averaged and are shown in Table 2.

[0079] Referring to the bactericidal effect detection method in Test Example 1, the sterilization rates of the disinfectants of Examples 1-6 and Comparative Examples 1-4 on three bacteria, Staphylococcus aureus (ATCC 6538), Escherichia coli (8099), and Pseudomonas aeruginosa (ATCC 15442), were calculated for 0.5 min, and the sterilization rates of the disinfectants of Examples 1-6 and Comparative Examples 1-4 on three bacteria, Staphylococcus aureus (ATCC 6538), Escherichia coli (8099), and Pseudomonas aeruginosa (ATCC 15442), were calculated for 0.5 min after being stored in a 54°C electric thermostat for 15 days; the sterilization reduction rate was calculated according to the sterilization rates before and after storage of the disinfectants, and the calculation formula for the sterilization reduction rate was as follows:

[0080] Sterilization reduction rate (%) = (C0-C) / C0×100%

[0081] Where C0 is the sterilization rate before storage, and C is the sterilization rate after storage.

[0082] The test results are averaged and are shown in Table 2.

[0083] Table 2 Effective iodine loss rate and sterilization reduction rate of each group of disinfectants

[0084]

[0085] As can be seen from Table 2, by comparing Examples 1-6 and Comparative Examples 1-4, it is found that the effective iodine loss rates of Examples 1-6 and Comparative Examples 2-4 containing cyclodextrin-iodine complexes are significantly lower than those of Comparative Example 1 not containing cyclodextrin-iodine complexes, indicating that the addition of cyclodextrin-iodine complexes can significantly reduce the effective iodine loss rate of the disinfectant and improve the stability of the disinfectant; by comparing Examples 1-6 and Comparative Examples 2-3, it is found that the effective iodine loss rates of Examples 1, Examples 5-6 and Comparative Example 2, in which amino-α-cyclodextrin is added to form a cyclodextrin-iodine complex, are lower than those of Examples 2-4 and Comparative Examples 3-4, indicating that compared with the addition of other cyclodextrin-iodine complexes, the cyclodextrin-iodine complex formed by adding amino-α-cyclodextrin has a more obvious effect of reducing the effective iodine loss rate of the disinfectant and has better stability.

[0086] In this test example, the sterilization reduction rate of the disinfectants of Examples 1-6 and Comparative Examples 1-4 after being stored in an electric thermostat at 54°C for 15 days was also detected. It can be seen from the test results in Table 2 that, by comparing Examples 1-6 and Comparative Examples 1-4, it is found that the sterilization reduction rates of Examples 1-6 and Comparative Examples 2-4 containing cyclodextrin-iodine complexes are significantly lower than those of Comparative Example 1 without cyclodextrin-iodine complexes, indicating that the addition of cyclodextrin-iodine complexes can significantly reduce the sterilization reduction rate of the disinfectant, improve the stability of the disinfectant, and maintain a good sterilization rate; by comparing Examples 1-6 and Comparative Examples 2-4, it is found that the sterilization reduction rates of Examples 1-6 and Comparative Examples 3-4 with the addition of stabilizers are significantly lower than those of Comparative Example 2 without stabilizers, and the sterilization reduction rate of Example 1 in which the stabilizer is erythritol is significantly lower than those of the Example in which the stabilizer is xylitol and Example 6 in which the stabilizer is mannitol, indicating that compared with xylitol and mannitol, erythritol has a more obvious effect of reducing the sterilization reduction rate of the disinfectant and a better effect of improving the stability of the disinfectant.

[0087] In summary, the cyclodextrin-iodine complex formed by adding amino-α-cyclodextrin to the disinfectant of the present invention and the coordinated use of erythritol, povidone iodine and composite quaternary ammonium salt are beneficial to reducing the effective iodine loss rate and sterilization reduction rate of the disinfectant during storage, which helps to maintain the bactericidal effect of the disinfectant, improve its stability and prolong its storage time.

[0088] Test Example 3

[0089] Skin irritation

[0090] According to method 2.3.3.1 of the Technical Specifications for Disinfectants (2002 Edition), the skin irritation test of the disinfectants of Examples 1-6 was carried out, and the local skin reaction was observed 1 hour, 24 hours and 48 hours after the test substance was removed. Combined with the scoring standard for skin irritation reaction and the skin irritation intensity grading standard provided by method 2.3.3 of the Technical Specifications for Disinfectants (2002 Edition), the skin irritation intensity of the disinfectants of Examples 1-6 was determined. The results are shown in Table 3.

[0091] Table 3 Skin irritation intensity

[0092]

[0093] From the skin irritation test results shown in Table 3, it can be seen that the disinfectants prepared in Examples 1-6 of the present invention are all safe and have no irritating effect on the skin.

Claims

1. A method for preparing a skin and mucous membrane disinfectant, characterized in that: The following steps are involved: According to the weight ratio of the proposed formula, 0.16% povidone iodine and 0.06% cyclodextrin-iodine complex are weighed, and then the povidone iodine and cyclodextrin-iodine complex are added to purified water in sequence, and mixed and stirred for 12 minutes to obtain a mixed solution; according to the weight ratio of the proposed formula, 0.20% complex quaternary ammonium salt and 1.5% erythritol are weighed, and then the complex quaternary ammonium salt is added to the mixed solution, and then erythritol is added and mixed and stirred for 15 minutes to obtain a skin and mucous membrane disinfectant; The preparation method of the cyclodextrin-iodine complex comprises the following steps, measured by weight: 1.8-2.2 parts of a cyclodextrin derivative are mixed with 0.3-0.5 parts of iodine powder to obtain a mixture; the mixture is placed in a container sealed with a polytetrafluoroethylene cover, and then placed in an oven at 55-65° C. for heat treatment for 6-8 hours; the container is cooled to room temperature, and the dark red solid in the container is placed at 23-27° C. for 10-12 hours to obtain a cyclodextrin-iodine complex; The cyclodextrin derivative is selected from one of amino-α-cyclodextrin, mono-6-O-amino-β-cyclodextrin, thiol-β-cyclodextrin and glucosyl-β-cyclodextrin.

2. The method for preparing a skin and mucous membrane disinfectant according to claim 1, wherein: The composite quaternary ammonium salt is selected from at least three of didodecyl dimethyl ammonium bromide, didodecyl dimethyl ammonium chloride, dioctyl dimethyl ammonium chloride, didecyl dimethyl ammonium bromide, didecyl dimethyl ammonium chloride, dodecyl dimethyl benzyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, dodecyl trimethyl ammonium chloride, dodecyl dimethyl-2-phenoxyethyl ammonium bromide, hexadecyl dimethyl benzyl ammonium bromide, and octadecyl dimethyl benzyl ammonium chloride.

3. The method for preparing a skin and mucous membrane disinfectant according to claim 1 or 2, wherein: The composite quaternary ammonium salt is prepared by mixing didodecyl dimethyl ammonium bromide, didecyl dimethyl ammonium bromide and dodecyl dimethyl-2-phenoxyethyl ammonium bromide in a weight ratio of 1:1-2:1-2.

4. A skin and mucous membrane disinfectant, characterized in that: Prepared by the method according to any one of claims 1 to 3.

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