Kiwifruit sprouting agent and method for cultivating kiwifruit renewal preparatory branches

Abstract

The present invention provides a kiwifruit sprouting agent and a method for cultivating kiwifruit regeneration preparatory branches. The kiwifruit sprouting agent comprises 6-benzylaminopurine, gibberellin, naphthaleneacetic acid, edible oil, water-based polyurethane, and water. Given the same amount of the sprouting agent, the present invention increases the effective usage amount and effective usage time of the sprouting agent, thereby shortening the time it takes for the plant to sprout.

Description

Technical Field

[0001] The invention belongs to the technical field of plant tissue culture, and particularly relates to a kiwifruit sprouting agent and a cultivation method for kiwifruit renewal preparatory branches. Background Art

[0002] Germination enhancers are agents that promote plant germination. Common germination enhancers include 6-benzylaminopurine, gibberellins, and naphthaleneacetic acid. They are applied to the desired germination area of ​​the plant to promote germination. After application, germination typically takes 8-10 days, but this can be a long process. Summary of the Invention

[0003] In view of this, the present invention provides a kiwifruit sprouting agent and a cultivation method for kiwifruit renewal preparatory branches, which increases the effective usage amount and effective usage time of the sprouting agent and shortens the time for plant sprouting.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In one aspect, the present invention provides a kiwifruit sprouting agent comprising: 6-benzylaminopurine, gibberellin, naphthaleneacetic acid, edible oil, water-based polyurethane, and water. It should be noted that the edible oil includes vegetable oil and animal oil, such as soybean oil, rapeseed oil, peanut oil, and may also be lard, sheep fat, etc.

[0006] Preferably, the volume ratio of edible oil, water-based polyurethane and water is: (1-4): (0.5-2): 50.

[0007] Preferably, the volume ratio of edible oil, water-based polyurethane and water is 2:1:50.

[0008] Preferably, the concentration of the 6-benzylaminoadenine is 800-1500 mg / L; and / or the concentration of the gibberellin is 40-80 mg / L; and / or the concentration of the naphthaleneacetic acid is 6-10 mg / L.

[0009] Preferably, the concentration of the 6-benzylaminoadenine is 1000 mg / L; and / or the concentration of the gibberellin is 60 mg / L; and / or the concentration of the naphthaleneacetic acid is 8 mg / L.

[0010] In another aspect, the present invention provides a method for cultivating kiwifruit renewal preparatory branches, comprising the following steps:

[0011] S100, after removing the main bud in the middle of the axillary bud, apply a healing agent to obtain a treated axillary bud, such as Figure 1 and 2 As shown;

[0012] S200, applying the kiwifruit sprouting agent on the treated axillary buds to obtain multiple secondary buds, such as Figure 3 As shown, Figure 3 This is the growth status diagram 5 days after the main bud was removed;

[0013] S300, removing the flower buds on each auxiliary bud, and allowing the auxiliary buds to grow after the flower buds are removed to obtain a plurality of preparatory branches.

[0014] Preferably, in step S100, after the main bud is cut off, the wound area left is 0.15-0.25 cm 2 It should be noted that the depth of the wound should be such that the cambium can be seen with the naked eye. For example, the depth of the wound can be between 1-2 mm. This operation is to destroy the main bud, break the apical dominance, and create conditions for the germination of the secondary buds.

[0015] Preferably, in step S200, the specific method of applying the germination agent is: applying once every 24-26 hours, and applying three times in total.

[0016] Preferably, in step S200, when each accessory bud grows to 3-8 cm, 2-4 of the plurality of accessory buds are retained, and the remaining accessory buds are removed, as shown in FIG. Figure 4-6 shown.

[0017] Preferably, when any one of the 2-4 retained secondary buds grows too vigorously and becomes a leggy branch, the leggy branch is pinched. It should be noted that removing the flower buds on the secondary buds facilitates the concentrated supply of nutrients to the branch growth, and pinching is adopted for the leggy branches that sprout too vigorously to promote secondary branches, so that each new branch forms a moderate branch with consistent growth and good growth. When the branches grow to half of the row spacing, they are pinched to the tip. After the leaves fall in winter, according to actual needs, the cultivated branches are trimmed to the required length, evenly arranged and tied to the wire. It should be noted that the row spacing in "when the branches grow to half of the row spacing" refers to the distance between every two rows of trees.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The present invention adds edible oil and water-based polyurethane to the sprouting agent. The water-based polyurethane is solidified mainly by the cohesive force and adhesion generated by the polar groups in the molecule. It has good bonding performance, good storage stability, coating mechanical properties, water resistance and aging resistance. The edible oil and water-based polyurethane together increase the viscosity of the sprouting agent, so that it can better adhere to the plant and increase the action time of the sprouting agent. Secondly, the naphthaleneacetic acid, gibberellin and 6-benzylaminoadenine in the sprouting agent are coated in the edible oil. When the sprouting agent is applied to the plant, the edible oil slowly releases the naphthaleneacetic acid, gibberellin and 6-benzylaminoadenine, which come into contact with the plant and promote the sprouting of the plant, further increasing the time the sprouting agent acts on the plant and avoiding the problem that part of the sprouting agent has volatilized before it acts on the plant. Under the same amount, the present invention increases the effective use amount and effective use time of the sprouting agent and shortens the time for the plant to sprout.

[0020] (2) This invention transforms a single bud into multiple buds, supplements the branching, increases the number of moderate branches, eliminates the emptiness of the inner chamber, and lays the foundation for cultivating the fruiting mother branches in the next year. The branches cultivated in the spring treatment grow more than 200 cm, the branch growth rate reaches 89%, the branches are strong, and the branch flowering rate reaches 98% in the following year. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A diagram of removing the main bud from the axillary bud provided by the present invention;

[0022] Figure 2 for Figure 1 Another angle of view;

[0023] Figure 3 for Figure 1 Growth status diagram 5 days after removing the main bud;

[0024] Figure 4 for Figure 1 Growth status diagram 10 days after removing the main bud;

[0025] Figure 5 for Figure 1 Growth status diagram 15 days after removing the main bud;

[0026] Figure 6 for Figure 1 Growth status diagram 30 days after removing the main bud. DETAILED DESCRIPTION

[0027] The present invention will be further described in detail below with reference to specific embodiments so that those skilled in the art can understand the present invention more clearly.

[0028] Example 1

[0029] Provided is a kiwifruit sprouting agent comprising: 20 mL soybean oil (Golden Arowana), 10 mL water-based polyurethane, 970 mL water, 800 mg 6-benzylaminoadenine, 40 mg gibberellin, and 6 mg naphthaleneacetic acid.

[0030] Example 2

[0031] Provided is a kiwifruit sprouting agent comprising: 38 mL of rapeseed oil (sunflower), 19 mL of water-based polyurethane, 943 mL of water, 1000 mg of 6-benzylaminoadenine, 60 mg of gibberellin, and 8 mg of naphthaleneacetic acid.

[0032] Example 3

[0033] Provided is a kiwifruit sprouting agent, comprising: 72 mL soybean oil (Golden Arowana), 36 mL water-based polyurethane, 892 mL water, 1500 mg 6-benzylaminoadenine, 80 mg gibberellin, and 10 mg naphthaleneacetic acid.

[0034] Comparative Example 1

[0035] Provided is a kiwi sprouting agent, the ingredients of which are similar to those in Example 1, except that the amount of edible oil added is 10 mL and the volume of water is 980 mL.

[0036] Comparative Example 2

[0037] A kiwi sprouting agent is provided, the ingredients of which are similar to those in Example 3, except that the amount of edible oil added is 80 mL and the volume of water is 884 mL.

[0038] Comparative Example 3

[0039] A kiwi sprouting agent is provided, the ingredients of which are similar to those in Example 1, except that the amount of waterborne polyurethane added is 5 mL and the volume of water is 975 mL.

[0040] Comparative Example 4

[0041] A kiwi sprouting agent is provided, the ingredients of which are similar to those of Example 3, except that the amount of waterborne polyurethane added is 40 mL and the volume of water is 888 mL.

[0042] Comparative Example 5

[0043] Provided is a kiwi sprouting agent, the ingredients of which are similar to those of Example 2, except that edible oil and water-based polyurethane are not added, and the volume of water is 1000 mL.

[0044] Comparative Example 6

[0045] A kiwi sprouting agent is provided, the ingredients of which are similar to those of Example 2, except that no water-based polyurethane is added and the volume of water is 962 mL.

[0046] Comparative Example 7

[0047] A kiwi sprouting agent is provided, the ingredients of which are similar to those in Example 2, except that no edible oil is added and the volume of water is 981 mL.

[0048] Application Example 1

[0049] A method for cultivating kiwifruit renewal preparatory branches is provided, comprising the following steps:

[0050] The first step is to deal with the bud point: use a blade to cut off the main bud in the middle of the axillary bud, cutting off an area of ​​about 0.2cm 2 The depth should be such that the cambium can be seen with the naked eye. Apply a wound healing agent to the incision, such as Figure 1 and 2 shown.

[0051] The second step is to cultivate the auxiliary buds: the axillary buds treated with the budding agent prepared in Example 1 are smeared once every 24 hours for 3 consecutive times. After about 5 days of treatment, the auxiliary buds sprout out. Figure 3 As shown, when the secondary buds grow to 3-8cm, keep 2-4 strong buds and remove the rest. Figure 4-6 shown.

[0052] The third step is to cultivate mature branches: remove the flower buds on the secondary buds and pinch off any overgrown branches to encourage secondary growth. Each new branch should grow uniformly and form a healthy, moderate branch. When the branches reach half the row spacing, pinch the tips. After the leaves fall in winter, the cultivated branches should be pruned to the desired length as needed and evenly arranged and tied to the wire.

[0053] Application Example 2-10

[0054] The cultivation method for kiwifruit renewal preparatory branches provided in Application Example 2-10 is similar to that in Application Example 1, except that the sprouting agents used in Application Examples 2-10 correspond to the sprouting agents of Examples 2-3 and Comparative Examples 1-7, respectively. For example, Application Example 2 uses the sprouting agent of Example 2, Application Example 5 uses the sprouting agent of Comparative Example 2, and Application Example 10 uses the sprouting agent of Comparative Example 7.

[0055] Comparative Application Example 1

[0056] Provided is a method for cultivating kiwifruit renewal preparatory branches, which is similar to Application Example 2, except that, in the second step, no budding agent is used.

[0057] Application Comparative Example 2

[0058] A method for cultivating kiwifruit renewal branches is provided, which is similar to Application Example 2, except that the main buds are not removed, and a budding agent is directly applied to the main buds, applied once every 24 hours, applied continuously for 3 times, and treated for about 5 days.

[0059] Application Comparative Example 3

[0060] Provided is a method for cultivating kiwifruit renewal preparatory branches, which is similar to Application Example 2, except that the main buds are not removed and the budding agent is not applied, and the control group is provided.

[0061] Performance testing and results

[0062] 1. Use the methods of application examples 1-11 and application comparison examples 1-3 to treat 10 axillary buds respectively, observe the budding time, budding agent residual time (days), average number of buds after each axillary bud treatment, moderate branch rate, weak branch rate and excessive branch rate of the application examples or application comparison examples. The results are shown in Table 1

[0063] Table 1

[0064]

[0065] Conclusion: Application Examples 1-3 achieved early bud break and an appropriate number of shoots, reducing the workload of manual bud removal. The budding agent's long shelf life provided a continuous supply of hormones to the shoots, resulting in high-quality buds, a high number of moderate shoots, and a low number of weak and leggy shoots. Reducing the amount of cooking oil or water-based polyurethane reduced the budding agent's adhesiveness, shortening its retention time, delaying bud break, reducing the number of shoots, and increasing leggy shoots. Adding cooking oil or water-based polyurethane caused the budding agent to remain on for too long, leading to an increase in the number of shoots, an increase in weak shoots, and a decrease in moderate shoots. In contrast, in Application Example 1, where no budding agent was used, bud break occurred later, the number of shoots was small, and branch growth was weak. In Application Example 2, where the main bud was not removed, apical dominance was effective, resulting in early bud break but a low number of shoots and a high number of leggy shoots. In Application Example 3, bud break was delayed, the number of shoots was small, and leggy shoots were high.

[0066] Through experimental comparison, it was found that destroying the main bud can increase the number of buds, reduce the proportion of long branches, and increase the proportion of medium branches. Applying budding agent can promote the germination of secondary buds, further increase the germination rate, reduce the proportion of weak branches, and increase the number of medium branches.

[0067] 2. Use the method of Application Example 2 and Application Comparative Examples 1-3 to update the preparatory branches. In spring, 50 axillary buds were treated with Application Example 2 and Application Comparative Examples 1-3 respectively, and the average branch length, branch growth rate and branching and flowering rate of the following year were observed. The results in spring are shown in Table 2.

[0068] Table 2

[0069]

[0070] Conclusion: The branches cultivated in Application Example 2 have moderate growth potential, sufficient nutrition, full new buds, and a high flowering rate in the following year. In Application Comparative Example 1, the main buds are removed without using a budding agent. The buds grow weakly and are not easy to form strong branches. The new buds do not accumulate nutrients enough and are not easy to flower in the following year. In Application Comparative Example 2, the main buds are not removed and only a budding agent is used. Although secondary buds germinate, the apical dominance is still there. The main buds grow too vigorously and easily become leggy branches. The flower buds are not fully differentiated and fewer flowers are formed in the following year. In Application Comparative Example 3, the main buds are neither removed nor used. The apical dominance of the main buds is stronger and easier to grow into leggy branches, and the flowering rate is lower in the following year.

[0071] The raw materials not specifically described in the present invention are all existing materials that can be directly purchased from the market.

[0072] The above is only a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for cultivating kiwifruit renewal branches, characterized in that: The following steps are involved: S100, after removing the main bud in the middle of the axillary bud, apply a healing agent to obtain a treated axillary bud; S200, applying a kiwi sprouting agent on the treated axillary buds to obtain multiple secondary buds; S300, removing the flower buds on each auxiliary bud, and allowing the auxiliary buds to grow after the flower buds are removed to obtain multiple preparatory branches; wherein, The kiwifruit sprouting agent comprises 6-benzylaminoadenine, gibberellin, naphthaleneacetic acid, edible oil, water-based polyurethane and water; The volume ratio of edible oil, water-based polyurethane and water is: (1-4): (0.5-2): 50; The concentration of the 6-benzylaminopurine is 800-1500 mg / L; The concentration of the gibberellin is 40-80 mg / L; and The concentration of the naphthaleneacetic acid is 6-10 mg / L.

2. The cultivation method according to claim 1, characterized in that In step S100, after the main bud is cut off, the wound area left is 0.15-0.25cm 2 .

3. The cultivation method according to claim 1, characterized in that In step S200, the specific method of applying the germination agent is: applying once every 24-26 hours, and applying a total of 3 times.

4. The cultivation method according to claim 1, characterized in that In step S200 , when each accessory bud grows to 3-8 cm, 2-4 of the plurality of accessory buds are retained, and the remaining accessory buds are removed.

5. The cultivation method according to claim 1, characterized in that When any one of the 2-4 retained secondary buds grows too vigorously and becomes a leggy branch, the leggy branch is pinched.

6. The cultivation method according to claim 1, characterized in that The volume ratio of edible oil, water-based polyurethane and water is 2:1:

50.

7. The cultivation method according to claim 1, characterized in that The concentration of the 6-benzylaminopurine is 1000 mg / L; The concentration of the gibberellin is 60 mg / L; The concentration of the naphthaleneacetic acid is 8 mg / L.

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