Burn dressing capable of preventing sepsis and preparation method thereof

Abstract

The invention relates to a burn dressing capable of preventing sepsis and a preparation method thereof. The dressing can be obtained through an extremely simple technological process, the imbibition / excipient, the antibacterial agent, the lipopolysaccharide inhibitor, the rheology regulator, the pH regulator, the osmotic pressure regulator, the humectant and other components can be directly dissolved in water to be mixed, packaged and sterilized, and then the dressing can be applied to promoting healing of burn wounds. While the antibacterial effect is achieved, signal transmission of lipopolysaccharide is inhibited by capturing lipopolysaccharide or competitively combining lipopolysaccharide receptors, and therefore sepsis mainly caused by lipopolysaccharide is prevented. The method is expected to play a positive promotion role in saving the life of a serious burn patient and relieving the burden of social and medical systems.

Description

Technical Field

[0001] The invention belongs to the field of wound management, relates to a burn dressing and a preparation method thereof, and particularly relates to a burn dressing capable of preventing sepsis and a preparation method thereof. Background Art

[0002] Skin is the largest organ in the human body and has many functions, such as protection, perception, absorption and temperature regulation. Therefore, the health of the skin should be given special attention. However, the skin is also the most vulnerable part of life. Skin trauma can be described as the destruction of the normal anatomical structure and function of the skin due to mechanical, chemical or thermal damage or potential physiological or medical conditions. Since all the physiological functions of the skin depend on the existence of an intact skin barrier, skin trauma is a serious threat to human health. In order to protect skin wounds and promote their healing, wound dressings were invented and applied. As people's understanding of the wound healing process continues to deepen, wound dressings have also undergone updates. Modern moist healing theory believes that an ideal wound dressing should have the following characteristics: covering sensitive underlying tissues, keeping the wound moist and absorbing a large amount of exudate, not adhering to the wound, and having good biocompatibility and degradability.

[0003] In addition to the above functions, wound dressings should also have antibacterial and antibacterial capabilities, because bacterial infection is the biggest obstacle to wound healing, especially for patients with severe burns, infection is one of the main causes of death. The most serious manifestation of infection is sepsis. The mortality rate of patients with sepsis ranges from 30% to 70%, and the number of sepsis patients worldwide exceeds 31.5 million per year. In addition, the cost of treating sepsis is high, which brings a huge economic burden to patients and society. Therefore, trying to prevent the occurrence of sepsis is of great significance to saving the lives of patients with severe burns and reducing the burden on the medical system. Sepsis is defined as a functional organ disorder that endangers life caused by a disorder of the host immune response caused by infection. The occurrence of sepsis involves a series of complex reactions between pathogenic microorganisms and the immune system. Among them, the large amount of lipopolysaccharide contained in the cell wall of Gram-negative bacteria is an important initiating factor in the occurrence of sepsis, which can induce pathological processes such as inflammatory response, coagulation disorder, and immunosuppression. Lipopolysaccharide mainly transmits signals through Toll-like receptor-4, leading to the massive release of proinflammatory cytokines such as interleukin-1β, interleukin-6, and tumor necrosis factor-α, forming an inflammatory factor storm, which ultimately leads to multiple organ dysfunction or even death in patients.

[0004] Since sepsis presents the characteristics of overwhelming inflammatory response in the early stage, the use of immunosuppressive therapy to reduce excessive inflammatory response has been regarded as the key to treating sepsis, such as applying anti-interleukin-6 antibodies, anti-tumor necrosis factor-α antibodies, etc., to antagonize inflammatory factors. Although this idea can reduce the level of inflammation in patients, it cannot effectively improve the clinical outcomes of patients. The reason is that although the inflammatory factor storm causes damage to the body, it is also an effective means to remove pathogens and lipopolysaccharides in the body. The silencing of the immune system causes the body to lose its most powerful weapon against pathogens, which is not conducive to the development of the disease. Designing targeted lipopolysaccharide inhibitors or antagonists for lipopolysaccharide and related receptor binding targets, blocking Toll-like receptor-4 signal transduction at different levels, reducing the signal stimulation received by the immune system without restricting its function, is a more fundamental treatment method currently generally recognized by the academic community. In the field of wound dressings, there is no precedent for using this method to prevent sepsis. Summary of the invention

[0005] In view of the above problems, the object of the present invention is to provide a burn dressing capable of preventing sepsis and a preparation method thereof.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A burn dressing capable of preventing sepsis comprises the following components per 100 parts by weight: 10-30 parts of an osmotic / excipient, 0.1-5 parts of an antibacterial agent, 0.1-5 parts of a lipopolysaccharide inhibitor, 0.5-3 parts of a rheology regulator, 0-2 parts of a pH regulator, 0.01-1.5 parts of an osmotic pressure regulator, 0-5 parts of a moisturizer and the balance of water.

[0008] Furthermore, the absorption / excipient is at least one of carbomer, sodium hyaluronate, polyethylene glycol, polyvinyl pyrrolidone, recombinant mussel mucin, recombinant collagen, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium alginate, fucoidan, β-glucan, carboxymethyl chitosan, trimethylsiloxysilicate, and acrylic acid copolymer.

[0009] Furthermore, the antibacterial agent is at least one of chitosan, silver ions (silver nitrate, silver sulfate, silver chloride, silver sodium zirconium phosphate, silver sulfadiazine), polylysine, p-hydroxyacetophenone, ethyl p-hydroxybenzoate, ethyl paraben, phenoxyethanol, potassium sorbate, and hypochlorous acid.

[0010] Furthermore, the lipopolysaccharide inhibitor is at least one of lipid A and its analogs, bactericidal / permeability-increasing protein and its analogs, berberine, and naloxone.

[0011] Furthermore, the rheology regulator is at least one of glycerin, vaseline, polydimethylsiloxane, and Tween 80.

[0012] Furthermore, the pH regulator is at least one of triethanolamine, citric acid, lactic acid, sodium dihydrogen phosphate, and disodium hydrogen phosphate.

[0013] Furthermore, the osmotic pressure regulator is at least one of sodium chloride, calcium chloride and potassium chloride.

[0014] Furthermore, the moisturizing agent is at least one of 1,2-hexanediol, trehalose and propylene glycol.

[0015] Furthermore, the preparation method of the burn dressing comprises the following steps: dissolving an absorbing / excipient, an antibacterial agent, a lipopolysaccharide inhibitor, a rheology regulator, a pH regulator, an osmotic pressure regulator and a moisturizer in an aqueous solution, transferring the solution into a container and sterilizing the container.

[0016] Compared with the prior art, the present invention has the following beneficial effects: the preparation process is simple and does not involve chemical synthesis; the antibacterial agent is loaded to kill wound bacteria; and the lipopolysaccharide inhibitor is loaded to selectively capture lipopolysaccharide or bind to lipopolysaccharide receptors, thereby hindering the signal transduction of lipopolysaccharide via Toll-like receptor-4, thereby preventing sepsis caused by lipopolysaccharide invading the body, promoting the smooth recovery of patients with large-area burns, and reducing medical and economic burdens. DETAILED DESCRIPTION

[0017] The technical solution of the present invention is further illustrated below in conjunction with embodiments, but these embodiments are not intended to limit the present invention.

[0018] Embodiment 1:

[0019] By weight, 15 parts of polyethylene glycol 400, 1 part of hyperbranched polylysine, 0.5 parts of human bactericidal / permeability-increasing protein, 3 parts of glycerol, 0.5 parts of citric acid, 0.9 parts of sodium chloride, and 2 parts of trehalose were dissolved in 78.1 parts of water, sterilized by high pressure, transferred to a soft tube, and sealed.

[0020] Embodiment 2:

[0021] By weight, 10 parts of carbomer, 1 part of carboxymethyl chitosan, 1 part of chitosan, 1 part of lipid A, 1.5 parts of polydimethylsiloxane, 1 part of triethanolamine, and 0.9 part of sodium chloride were dissolved in 83.6 parts of water, sterilized by irradiation, transferred to a soft tube, and sealed.

[0022] Embodiment 3:

[0023] By weight, 10 parts of sodium carboxymethylcellulose and 3.5 parts of acrylic acid copolymer, 0.8 parts of p-hydroxyacetophenone, 1 part of naloxone, 2 parts of Tween 80, 0.8 parts of sodium chloride and 0.02 parts of calcium chloride, and 1.5 parts of propylene glycol were dissolved in 80.38 parts of water, sterilized by autoclave, transferred to a soft tube, and sealed.

[0024] Embodiment 4:

[0025] By weight, 30 parts of sodium hyaluronate, 0.1 parts of phenoxyethanol, 5 parts of berberine, 3 parts of glycerin, 2 parts of sodium dihydrogen phosphate, 0.9 parts of potassium chloride, and 1 part of 1,2-hexanediol were dissolved in 58 parts of water, sterilized by autoclave, transferred to a soft tube, and sealed.

[0026] Embodiment 5:

[0027] By weight, 15 parts of sodium alginate, 2 parts of β-glucan, 5 parts of potassium sorbate, 2 parts of lipid A, 0.5 parts of vaseline, 2 parts of triethanolamine, and 1 part of sodium chloride were dissolved in 72.5 parts of water, sterilized by irradiation, transferred to a soft tube, and sealed.

[0028] Embodiment 6:

[0029] By weight, 10 parts of sodium carboxymethylcellulose and 5 parts of hydroxyethylcellulose, 2 parts of p-hydroxyacetophenone, 1 part of naloxone, 2 parts of Tween 80, 0.5 parts of sodium chloride, and 1.5 parts of propylene glycol were dissolved in 78 parts of water, sterilized by autoclave, transferred into a soft tube, and sealed.

[0030] In addition, the product of the present invention can also be made into ointments, gels, suspensions, etc. according to conventional methods, and can be directly applied to burn wounds, all of which have better effects, can promote the healing of burn wounds, and effectively prevent sepsis.

[0031] Those skilled in the art understand that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A burn dressing capable of preventing sepsis, It is characterized in that By weight, the components in every 100 parts are as follows: 10-30 parts of osmotic / excipient, 0.1-5 parts of antibacterial agent, 0.1-5 parts of lipopolysaccharide inhibitor, 0.5-3 parts of rheology regulator, 0-2 parts of pH regulator, 0.01-1.5 parts of osmotic pressure regulator, 0-5 parts of moisturizer and the balance of water.

2. The burn dressing according to claim 1, It is characterized in that The absorption / excipient is at least one of carbomer, sodium hyaluronate, polyethylene glycol, polyvinyl pyrrolidone, recombinant mussel mucin, recombinant collagen, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium alginate, fucoidan, β-glucan, carboxymethyl chitosan, trimethylsiloxysilicate, and acrylic acid copolymer.

3. The burn dressing according to claim 1, It is characterized in that The antibacterial agent is at least one of chitosan, silver nitrate, silver sulfate, silver chloride, silver sodium zirconium phosphate, silver sulfadiazine, polylysine, p-hydroxyacetophenone, ethyl p-hydroxybenzoate, ethyl paraben, phenoxyethanol, potassium sorbate and hypochlorous acid.

4. The burn dressing according to claim 1, It is characterized in that The lipopolysaccharide inhibitor is at least one of lipid A and its analogs, bactericidal / permeability-increasing protein and its analogs, berberine, and naloxone.

5. The burn dressing according to claim 1, It is characterized in that The rheology regulator is at least one of glycerin, vaseline, polydimethylsiloxane and Tween 80.

6. The burn dressing according to claim 1, It is characterized in that The pH regulator is at least one of triethanolamine, citric acid, lactic acid, sodium dihydrogen phosphate and disodium hydrogen phosphate.

7. The burn dressing according to claim 1, It is characterized in that The osmotic pressure regulator is at least one of sodium chloride, calcium chloride and potassium chloride.

8. The burn dressing according to claim 1, It is characterized in that The moisturizing agent is at least one of 1,2-hexanediol, trehalose and propylene glycol.

9. The burn dressing according to claim 1, It is characterized in that The preparation method comprises the following steps: dissolving an osmotic / excipient, an antibacterial agent, a lipopolysaccharide inhibitor, a rheology regulator, a pH regulator, an osmotic pressure regulator and a moisturizer in an aqueous solution, transferring the solution into a container and sterilizing the container.

10. The burn dressing capable of preventing sepsis according to any one of claims 1 to 9, It is characterized in that The dressing is applied by direct smearing, spraying or aseptic gauze dipping, and can kill wound pathogens while inhibiting the inflammatory response caused by lipopolysaccharide contained in Gram-negative bacteria, thereby promoting wound healing and preventing the occurrence of sepsis.