Breeding method and application of morchella pinnata

Abstract

The invention discloses a breeding method and application of morchella pinnata. According to the method, literature research and technical evaluation are firstly carried out to provide reference for follow-up work; then, research preparation is carried out on a growth site, and a wild morchella conestris collection area is determined; wild fungi are collected to obtain spore powder, quality detection is carried out, and then single conidium is obtained and subjected to pure culture and transfer amplification culture. And then carrying out primary culture test, screening a culture medium formula and culture conditions, producing a secondary strain, and carrying out quality evaluation. And finally obtaining a target strain through an artificial cultivation test. The breeding method has systematicness and scientificity, strictly controls the quality of each link and improves the breeding efficiency. By optimizing the culture medium and culture conditions, it is ensured that morchella conestris is high in growth speed, large in biomass, good in shape and high in stability. The bred morchella conestris is popularized and planted in a large area, and has wide market prospects and economic values.

Description

Technical Field

[0001] The invention relates to the technical field of Morchella breeding, in particular to a breeding method and application of a pointed-top Morchella. Background Art

[0002] Morchella is a precious edible mushroom with high nutritional and economic value. As a type of Morchella, Morchella apex has attracted much attention for its unique flavor and nutritional value. However, the current breeding methods of Morchella apex have certain limitations.

[0003] Existing breeding technologies may lack systematicity and scientificity, and insufficient understanding of the growth environment of wild Morchella esculenta may result in unsatisfactory performance of the selected strains in terms of adaptability, yield and quality.

[0004] Therefore, a more scientific and efficient method for breeding pointed-top morels is needed to meet the market demand for high-quality pointed-top morels. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a scientific and efficient breeding method and application of pointed-top morel.

[0006] The breeding method of a pointed-top morel of the present invention comprises the following steps:

[0007] S1: Literature research and technology assessment;

[0008] S2: Preparation for research on the growing area;

[0009] S3: Collect wild Morchella esculenta to obtain spore powder;

[0010] S4: spore powder quality inspection;

[0011] S5: Obtain a single conidia for pure culture;

[0012] S6: Transfer and expand cultivation;

[0013] S7: preliminary culture test;

[0014] S8: Screening of culture medium formulation and culture conditions;

[0015] S9: Produce secondary species;

[0016] S10: Secondary seed quality assessment;

[0017] S11: Conduct artificial cultivation experiments to obtain the target strain.

[0018] Preferably, the step S1 specifically includes extensively collecting research literature and successful cases on the breeding of Morchella cuspidata at home and abroad, understanding the latest breeding technologies and methods, evaluating different breeding technologies, and providing reference for subsequent breeding work;

[0019] The step S2 specifically includes:

[0020] (a) Conduct detailed ecological and environmental research on the growing area and keep good records;

[0021] (b) Cooperate with local nature conservation agencies, forestry departments or residents in the survey area to obtain clues about the growth of wild Morchella esculenta;

[0022] (c) Identify specific areas where wild pointed morels are most likely to be found.

[0023] Preferably, the collection of wild Morchella cuspidata in step S3 specifically includes:

[0024] (a) Use professional collection tools to collect wild pointed-top morels from their growing areas;

[0025] (b) Number and record the wild Morchella esculenta collected, including the collection location, time and environmental characteristics;

[0026] The specific steps of obtaining spore powder in step S3 are:

[0027] (a) placing the collected wild morels on a sterile operating table;

[0028] (b) Use a sterile scalpel or forceps to open the cap and expose the gills;

[0029] (c) Use a sterile brush or cotton swab to collect the spores on the gills and place them in a sterile container;

[0030] (d) This can be repeated multiple times to ensure that enough spores are collected;

[0031] (e) Filter through a sterile filter to remove impurities and residual tissue fragments to obtain spore powder.

[0032] Preferably, in step S4, the detection indicators of the spore powder include purity, vitality and genetic diversity; the spore powder that meets the quality standards proceeds to the next step, and the spore powder that does not meet the quality standards needs to be recollected.

[0033] Preferably, the steps of step S5 specifically include:

[0034] (a) Choose appropriate smearing tools such as sterile glass rods, toothpicks or micropipette, and sterilize them strictly;

[0035] (b) when preparing the culture medium, adding plant growth regulators, vitamins or amino acid components, optimizing the pH value of the culture medium, and using a double-layer culture medium method;

[0036] (c) Take a small amount of spore powder on a sterile operating table and gently smear it on the surface of the culture medium. Use a microscope to observe whether there are single conidia. Multiple smearing operations can be performed to increase the probability of obtaining single conidia.

[0037] (d) Control the temperature, humidity and light conditions of the culture environment, regularly observe the growth of conidia on the surface of the culture medium, promptly remove conidia that contaminate the culture dish and grow poorly, and use liquid culture to accelerate the growth of conidia;

[0038] (e) Obtain target strains with specific shapes and excellent quality.

[0039] Preferably, the formula of the culture medium used in step S6 is specifically: 200 grams of potatoes, 20 grams of glucose, 22 grams of agar powder, 3 grams of peptone, 1 gram of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 1 gram of beef extract, and 1000 grams of purified water;

[0040] The specific culture conditions are: temperature 20℃, humidity 65%, carbon dioxide concentration less than 1500PPM; one week of culture to obtain the first-class seed;

[0041] When transferring, use sterile inoculation tools, adopt the slant transfer method and record the culture number after transfer.

[0042] Preferably, the specific steps of the preliminary culture test in step S7 are:

[0043] (a) Preparation: Collect different types of culture media, including but not limited to existing commonly used culture media and new culture media that may be suitable for the growth of Morchella apicalis obtained based on previous research and literature research; determine different combinations of culture conditions, culture condition temperature range, humidity range and light intensity;

[0044] (b) inoculation and cultivation: a certain number of strains are selected from the pure cultured Morchella apical, and are inoculated into different culture media respectively; the inoculated culture media are placed under different culture conditions for cultivation, and incubators with different temperature, humidity and light conditions are set;

[0045] (c) Observation and recording: Regularly observe the growth of Morchella esculenta in different culture media and culture conditions, including growth rate, colony morphology and mycelial density; record the growth performance of Morchella esculenta under different combinations, including the time of starting growth and the characteristics of the vigorous growth period;

[0046] (d) Result analysis: Based on the observed data, the effects of different culture media and culture conditions on the growth of Morchella ogee were analyzed; some combinations of culture media and culture conditions with better performance were preliminarily determined to provide a reference for subsequent screening work.

[0047] Preferably, the specific steps of screening the culture medium formula and culture conditions in step S8 are:

[0048] (a) Experimental design: Orthogonal experimental design or response surface analysis was used to determine the factors and levels to be investigated, with carbon source, nitrogen source, temperature, humidity and light intensity as factors, and different levels were set;

[0049] (b) Culture medium preparation: Prepare culture medium with different formulations according to the experimental design, ensuring that the components of each formulation are accurately weighed and mixed evenly;

[0050] (c) Cultivation and observation: The pointed-top morel was inoculated into culture media with different formulations and cultured under different set culture conditions; the growth of the morel was regularly observed, and the growth rate, biomass and mycelial morphology indicators were measured;

[0051] (d) Data analysis: Statistical analysis methods were used to analyze the influence of different factors on the growth of Morchella; the best combination of culture medium formula and culture conditions was determined, which could make Morchella grow at the fastest rate, with the largest biomass, good morphology and high stability.

[0052] Preferably, the specific steps of producing the secondary seed in step S9 are:

[0053] Prepare the raw materials for the culture bag: white wheat 70%, sawdust 10%, rice husk 10%, humus 5%, plant ash 3%, lime 1%, gypsum 1%, water content 55%, zinc 1-3mg / L, cytokinin 0.5-1.5mg / L;

[0054] Bagging and sterilization: put the mixed culture medium into a specific strain bag and seal it tightly; sterilize it with high-pressure steam sterilization to ensure that there is no contamination of bacteria in the culture medium;

[0055] Inoculation and culture: Under sterile conditions, inoculate the selected and expanded Morchella esculenta strains into the secondary seed culture bag for culture, and observe the growth of the strains regularly;

[0056] The secondary seed quality assessment indicators of step S10 include: appearance inspection, growth assessment, vitality detection and stress resistance assessment.

[0057] The invention discloses an application of a pointed-top morel, wherein the pointed-top morel is bred by using the method for breeding a pointed-top morel as claimed in any one of claims 1 to 9, and the pointed-top morel is widely planted.

[0058] Compared with the prior art, the present invention has the following beneficial effects:

[0059] 1. Highly systematic: Starting from the early literature research and technical evaluation, we widely collect advanced data from home and abroad to lay a solid theoretical foundation for the entire breeding process. Then we conduct a comprehensive survey and preparation of the growing area, covering ecological environment surveys, cooperation with relevant institutions and residents, and determination of precise collection areas, etc., to provide a clear direction for subsequent work.

[0060] 2. Highlighting strong scientificity: The research on the growing area is meticulous and detailed, fully understanding the growth environment of wild Morchella esculenta. The experimental design screens the culture medium formula and culture conditions, and uses scientific methods such as orthogonal experimental design or response surface analysis to determine the factors and levels to be investigated to ensure the accuracy of the culture conditions.

[0061] 3. Effectively ensure the quality of breeding: The collected wild Morchella esculenta are numbered and recorded in detail to facilitate the traceability and analysis of strain characteristics. Strict spore powder testing steps, including purity, vitality and genetic diversity testing, ensure the high quality of breeding materials. The purity and quality of the target strain are improved through pure culture of single conidia and optimization of culture medium and culture conditions.

[0062] 4. Significantly improve breeding efficiency: collect different culture media and determine the combination of culture conditions in the preliminary culture test, observe and record after inoculation and culture, and analyze and determine the best combination. Use statistical analysis methods when screening culture medium formulas and culture conditions to quickly determine the best combination of culture conditions, greatly shortening the breeding time and improving efficiency.

[0063] 5. Ensure the quality of secondary strains: strictly control the production process of secondary strains, from the preparation of culture bag raw materials to bagging sterilization and inoculation culture, to ensure that there is no contamination by foreign bacteria. Comprehensive quality assessment indicators, covering appearance inspection, growth assessment, vitality detection and stress resistance assessment, provide high-quality strains for the promotion of planting.

[0064] 6. Show broad market prospects: The pointed-top morels obtained through this breeding method are of excellent quality and can be promoted for large-scale cultivation, meeting the market's urgent demand for high-quality pointed-top morels. It has huge economic value and broad market development space. BRIEF DESCRIPTION OF THE DRAWINGS

[0065] Figure 1 It is a flow chart of a breeding method of a pointed-top morel of the present invention; DETAILED DESCRIPTION

[0066] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0067] like Figure 1 As shown, the specific implementation steps of the method for breeding the pointed morel are as follows:

[0068] Literature Research and Technology Assessment:

[0069] We have extensively collected research literature and successful cases on the breeding of Morchella cuspidata at home and abroad, and gained an in-depth understanding of the latest breeding technologies and methods. We have comprehensively evaluated different breeding technologies, analyzed their advantages and disadvantages and applicable scope, and provided important reference for subsequent breeding work.

[0070] Preparation for the research on the growing area:

[0071] Detailed ecological environment survey and record of the growing area: Conduct a comprehensive and in-depth ecological environment survey in Jiuzhaigou County, Aba Prefecture, Sichuan Province. Record the specific forest types, altitude, soil conditions and other information in the area in detail, while paying attention to geographic coordinates, vegetation characteristics and climate characteristics. These records will provide accurate guidance for subsequent wild morel collection.

[0072] Cooperate with local nature conservation agencies, forestry departments or residents in the survey area to obtain clues about the growth of wild pointed-top morels: Actively cooperate with local nature conservation agencies, forestry departments and residents familiar with the local terrain. Obtain clues from them about the growth locations of wild pointed-top morels to improve the pertinence and efficiency of collection.

[0073] Determine the specific areas where wild pointed-top morels are most likely to be found: Based on ecological and environmental surveys and clues obtained, determine the specific areas where wild pointed-top morels are most likely to be found, and make full preparations for subsequent collection work.

[0074] Collecting wild Morchella cuspidata to obtain spore powder: On May 8, 2017, a fresh wild Morchella (No. 17JZG-12) was collected using professional collection tools in Jiuzhaigou County, Aba Prefecture, Sichuan Province. The collected wild Morchella was placed on a sterile operating table, and the cap was opened with a sterile scalpel or tweezers to expose the gills. Then, the spores on the gills were brushed with a sterile brush or cotton swab and collected in a sterile container. This process can be repeated several times to ensure that enough spore powder is collected. Finally, impurities and residual tissue fragments were filtered through a sterile filter to obtain pure spore powder. The spore powder was obtained by natural air drying.

[0075] Spore powder quality inspection:

[0076] The purity, vitality and genetic diversity of the spore powder are tested. Only spore powder that meets the quality standards can enter the next step. Spore powder that does not meet the quality standards needs to be collected again.

[0077] Obtaining single conidia for pure culture:

[0078] Select appropriate smear tools and disinfect them: Select appropriate smear tools such as sterile glass rods, toothpicks or micropipette, and strictly disinfect them to ensure that no bacteria are introduced during the operation.

[0079] Optimize culture medium composition and methods: Add plant growth regulators, vitamins or amino acid components when preparing the culture medium, optimize the pH value of the culture medium, and use a double-layer culture medium method to provide a good growth environment for conidia.

[0080] Smearing operation and observation: Take a small amount of spore powder on the sterile operating table and gently smear it on the surface of the culture medium. Use a microscope to observe whether there are single conidia. Multiple smearing operations can be performed to increase the probability of obtaining single conidia.

[0081] Control the culture environment and deal with adverse conditions: Control the temperature, humidity and light conditions of the culture environment, and regularly observe the growth of conidia on the surface of the culture medium. Remove conidia that contaminate the culture dish and grow poorly in time, and use liquid culture to accelerate the growth of conidia.

[0082] Obtaining the target strain: The strain (No. JZW-0012) was obtained through pure culture, and was determined to be the Mel-6 variety through identification, obtaining the target strain with a specific shape and excellent quality.

[0083] Transfer and expand cultivation:

[0084] In September 2017, the culture medium was used for transfer and expansion culture. The culture medium formula was 200 grams of potato, 20 grams of glucose, 22 grams of agar powder, 3 grams of peptone, 1 gram of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 1 gram of beef extract, and 1000 grams of purified water. The culture conditions were temperature 20°C, humidity 65%, and carbon dioxide concentration less than 1500PPM. The first-class species was obtained after one week of cultivation. The strain showed dense mycelium, neat growth, fast growth rate, strong sclerotium production ability, and strong anti-aging ability when growing in the culture medium. Sterile inoculation tools were used for transfer, and the slant transfer method was adopted, and the transferred cultures were numbered and recorded.

[0085] Initial culture test:

[0086] Preparation: Collect different types of culture media, including existing commonly used culture media and new culture media that may be suitable for the growth of Morchella apicalis based on previous research and literature research. Determine different combinations of culture conditions, including the culture temperature range, humidity range and light intensity.

[0087] Inoculation and cultivation: A certain number of strains are selected from the pure cultured Morchella apicalis and inoculated into different culture media. The inoculated culture media are placed under different culture conditions for cultivation, and incubators with different temperature, humidity and light conditions are set.

[0088] Observation and Recording: Regularly observe the growth of Morchella apicalis in different culture media and culture conditions, including growth rate, colony morphology and mycelium density. Record the growth performance of Morchella apicalis under different combinations, including the time of starting growth and the characteristics of the vigorous growth period.

[0089] Result analysis: Based on the observed data, the effects of different culture media and culture conditions on the growth of Morchella apex were analyzed. Some combinations of culture media and culture conditions with better performance were preliminarily determined to provide reference for subsequent screening work.

[0090] Screening medium formulation and culture conditions:

[0091] Experimental design: Orthogonal experimental design or response surface analysis was used to determine the factors and levels to be investigated, with carbon source, nitrogen source, temperature, humidity and light intensity as factors, and different level values ​​were set.

[0092] Culture medium preparation: Prepare culture medium with different formulations according to the experimental design, ensuring that the ingredients of each formulation are accurately weighed and mixed evenly.

[0093] Cultivation and observation: The pointed-top morel was inoculated into culture media with different formulations and cultured under different set culture conditions. The growth of the morel was regularly observed, and the growth rate, biomass, and mycelial morphology were measured.

[0094] Data analysis: Statistical analysis methods were used to analyze the influence of different factors on the growth of Morchella. The best combination of culture medium formula and culture conditions was determined, which can make Morchella grow fastest, have the largest biomass, good morphology and high stability.

[0095] Production of secondary species:

[0096] The formula selected for secondary seed production is: 70% white wheat, 10% sawdust, 10% rice husk, 5% humus, 3% plant ash, 1% lime, 1% gypsum, 55% water content, 2mg / L zinc, 1mg / L cytokinin. Put the mixed culture into a specific strain bag and seal it well. Sterilize it with high-pressure steam sterilization to ensure that there is no contamination of bacteria in the culture. Under sterile conditions, the selected and expanded morel strains are inoculated into the secondary strain culture bag for cultivation, and the growth of the strains is observed regularly.

[0097] Secondary seed quality assessment:

[0098] The secondary seeds are subjected to appearance inspection, growth assessment, vitality test and stress resistance assessment to ensure that their quality meets the requirements.

[0099] Conduct artificial cultivation experiments to obtain the target strain:

[0100] According to the cultivation method described in the invention patent (ZL201510332106.6) previously authorized by the applicant, artificial cultivation experiments were carried out at the planting base in Chujiang Town, Dayi County, Chengdu City, Sichuan Province, and mushrooms were obtained. Artificially cultivated fruiting bodies were collected to obtain the target strain (No. JZW-1712). The strain is pagoda-shaped, with a slightly pointed top, black color, thick flesh, clear and tight lines, 7-12 cm mushroom cap, and 1-3 cm mushroom foot. After drying, the delivery rate was measured to be 6.8, which is 15% better than the conventional six sisters, and has a strong fragrance and does not fall off.

[0101] 2. Application and Implementation Methods of Spire Morel

[0102] The pointed-top morel is bred using the above-mentioned breeding method, and during the process of promotion and application, it shows the characteristics of stable yield and strong resistance to foreign bacteria, and has significant economic benefits and market value.

[0103] In 2018, a comparative cultivation experiment was conducted in Dayi County, Chengdu, Yanting County, Mianyang, Laifeng County, Enshi, Yichuan County, Zunyi, and Jinsha County, Bijie. The control strain was the variety approved by the Sichuan Academy of Agricultural Sciences: Chuanyang No. 1 (variety right number: CNA20170039.2). The formula used in the experiment was 70% white wheat, 10% sawdust, 10% rice husk, 5% humus, 3% plant ash, 1% lime, 1% gypsum, 55% water content, 2mg / L zinc, and 1mg / L cytokinin. The cultivation steps were to apply 150kg of quicklime powder per mu 15 days before sowing, plow 2-3 times until the soil particles were less than 5 cm, and mix the lime evenly for better insecticide and sterilization; crush the secondary seeds into 1-2 cm particles, use 0.5kg of seeds per square meter, and evenly spread them on the soil surface, covering the soil with 3-5 cm. The conditions for spawning are: temperature 8-15℃, humidity 50-60%, light 1000lx, carbon dioxide concentration below 1000PPM, and maintain for 40-50 days. The conditions for fruiting are: temperature 10-20℃, humidity 80-90%, light 1500-2000lx, carbon dioxide concentration below 1000PPM, and maintain for 20-30 days until the end of mushroom harvesting. The target strain (No. JZW-1712) and the control strain Chuanyang No. 1 (variety right No.: CNA20170039.2) are all grown under the same conditions. The only difference is the strain itself.

[0104] The cultivation results are shown in the following table:

[0105]

[0106] From the results, we can see that the growth time of the target strain is 7 days shorter than that of the control strain, indicating that the target strain has stronger adaptability to the environment, faster mycelium growth rate, and stronger ability to decompose and absorb nutrients. The target strain has a longer mushroom picking cycle than the control strain, which helps to obtain more and larger fruiting bodies. The average yield of the target strain in 5 areas is 65 kg higher than that of the control strain. According to the market price of 150 yuan, the income per mu will increase by 10,000 yuan. At the same time, the highest yield is 124 kg higher than that of the control strain, indicating that the target strain has greater yield potential. The proportion of large mushrooms in the target strain is twice that of the control strain. According to the yield of 500 kg of fresh mushrooms per mu, the output value of the target strain per mu will increase by about 3,000 yuan.

[0107] From 2019 to 2023, the promotion will be carried out in Sichuan, Guizhou, Hubei, Yunnan, Henan, Anhui and other places. In the application in different regions, appropriate adjustments will be made according to the actual local conditions to ensure that the pointed morel can adapt to the local environment. Strengthen cooperation with purchasers to improve the product's visibility and market competitiveness. Continuously summarize the promotion experience, further optimize the breeding and cultivation techniques, and improve the yield and quality of pointed morel. In the past five years, the cumulative promotion area has exceeded 20,000 mu, with an output value of about 400 million yuan.

[0108] Through the above specific implementation methods, the breeding and application of pointed-top morel can be effectively realized, providing strong technical support for the industrial development of pointed-top morel.

[0109] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for breeding a pointed-top morel. It is characterized in that The following steps are involved: S1: Literature research and technology assessment; S2: Preparation for research on the growing area; S3: Collect wild Morchella esculenta to obtain spore powder; S4: spore powder quality inspection; S5: Obtain a single conidia for pure culture; S6: Transfer and expand cultivation; S7: preliminary culture test; S8: Screening of culture medium formulation and culture conditions; S9: Produce secondary species; S10: Secondary seed quality assessment; S11: Conduct artificial cultivation experiments to obtain the target strain.

2. The method for breeding a pointed-top morel according to claim 1, It is characterized in that The step S1 specifically includes extensively collecting research literature and successful cases on the breeding of pointed morels at home and abroad, understanding the latest breeding techniques and methods, evaluating different breeding techniques, and providing reference for subsequent breeding work; The step S2 specifically includes: (a) Conduct detailed ecological and environmental research on the growing area and keep good records; (b) Cooperate with local nature conservation agencies, forestry departments or residents in the survey area to obtain clues about the growth of wild Morchella esculenta; (c) Identify specific areas where wild pointed morels are most likely to be found.

3. The method for breeding a pointed-top morel according to claim 1, It is characterized in that The step S3 of collecting wild Morchella cuspidata specifically includes: (a) Use professional collection tools to collect wild pointed-top morels from their growing areas; (b) Number and record the wild Morchella esculenta collected, including the collection location, time and environmental characteristics; The specific steps of obtaining spore powder in step S3 are: (a) placing the collected wild morels on a sterile operating table; (b) Use a sterile scalpel or forceps to open the cap and expose the gills; (c) Use a sterile brush or cotton swab to collect the spores on the gills and place them in a sterile container; (d) This can be repeated multiple times to ensure that enough spores are collected; (e) Filter through a sterile filter to remove impurities and residual tissue fragments to obtain spore powder.

4. The method for breeding a pointed-top morel according to claim 1, It is characterized in that In step S4, the detection indicators of the spore powder include purity, vitality and genetic diversity; the spore powder that meets the quality standards proceeds to the next step, and the spore powder that does not meet the quality standards needs to be recollected.

5. The method for breeding a pointed-top morel according to claim 1, It is characterized in that The steps of step S5 specifically include: (a) Choose appropriate smearing tools such as sterile glass rods, toothpicks or micropipette, and sterilize them strictly; (b) when preparing the culture medium, adding plant growth regulators, vitamins or amino acid components, optimizing the pH value of the culture medium, and using a double-layer culture medium method; (c) Take a small amount of spore powder on a sterile operating table and gently smear it on the surface of the culture medium. Use a microscope to observe whether there are single conidia. Multiple smearing operations can be performed to increase the probability of obtaining single conidia. (d) Control the temperature, humidity and light conditions of the culture environment, regularly observe the growth of conidia on the surface of the culture medium, promptly remove conidia that contaminate the culture dish and grow poorly, and use liquid culture to accelerate the growth of conidia; (e) Obtain target strains with specific shapes and excellent quality.

6. The method for breeding a pointed-top morel according to claim 1, It is characterized in that The formula of the culture medium used in step S6 is specifically: 200 grams of potato, 20 grams of glucose, 22 grams of agar powder, 3 grams of peptone, 1 gram of potassium dihydrogen phosphate, 1 gram of magnesium sulfate, 1 gram of beef extract, and 1000 grams of purified water; The specific culture conditions are: temperature 20℃, humidity 65%, carbon dioxide concentration less than 1500PPM; one week of culture to obtain the first-class seed; When transferring, use sterile inoculation tools, adopt the slant transfer method and record the culture number after transfer.

7. The method for breeding a pointed-top morel according to claim 1, It is characterized in that The specific steps of the preliminary culture test in step S7 are: (a) Preparation: Collect different types of culture media, including but not limited to existing commonly used culture media and new culture media that may be suitable for the growth of Morchella apicalis obtained based on previous research and literature research; determine different combinations of culture conditions, culture condition temperature range, humidity range and light intensity; (b) inoculation and cultivation: a certain number of strains are selected from the pure cultured Morchella apical, and are inoculated into different culture media respectively; the inoculated culture media are placed under different culture conditions for cultivation, and incubators with different temperature, humidity and light conditions are set; (c) Observation and recording: Regularly observe the growth of Morchella esculenta in different culture media and culture conditions, including growth rate, colony morphology and mycelial density; record the growth performance of Morchella esculenta under different combinations, including the time of starting growth and the characteristics of the vigorous growth period; (d) Result analysis: Based on the observed data, the effects of different culture media and culture conditions on the growth of Morchella ogee were analyzed; some combinations of culture media and culture conditions with better performance were preliminarily determined to provide a reference for subsequent screening work.

8. The method for breeding a pointed-top morel according to claim 1, It is characterized in that The specific steps of screening the culture medium formula and culture conditions in step S8 are: (a) Experimental design: Orthogonal experimental design or response surface analysis was used to determine the factors and levels to be investigated, with carbon source, nitrogen source, temperature, humidity and light intensity as factors, and different levels were set; (b) Culture medium preparation: Prepare culture medium of different formulations according to the experimental design, ensuring that the components of each formulation are accurately weighed and mixed evenly; (c) Cultivation and observation: The pointed-top morel was inoculated into culture media with different formulations and cultured under different set culture conditions; the growth of the morel was regularly observed, and the growth rate, biomass and mycelial morphology indicators were measured; (d) Data analysis: Statistical analysis methods were used to analyze the influence of different factors on the growth of Morchella; the best combination of culture medium formula and culture conditions was determined, which could make Morchella grow at the fastest rate, with the largest biomass, good morphology and high stability.

9. The method for breeding a strain of Morchella apicalis according to claim 1, It is characterized in that The specific steps of producing the secondary seed in step S9 are: Prepare the raw materials for the culture bag: white wheat 70%, sawdust 10%, rice husk 10%, humus 5%, plant ash 3%, lime 1%, gypsum 1%, water content 55%, zinc 1-3mg / L, cytokinin 0.5-1.5mg / L; Bagging and sterilization: put the mixed culture medium into a specific strain bag and seal it tightly; sterilize it with high-pressure steam sterilization to ensure that there is no contamination of bacteria in the culture medium; Inoculation and culture: Under sterile conditions, inoculate the selected and expanded Morchella esculenta strains into the secondary seed culture bag for culture, and observe the growth of the strains regularly; The secondary seed quality assessment indicators of step S10 include: appearance inspection, growth assessment, vitality detection and stress resistance assessment.

10. An application of pointed-top morel, It is characterized in that The pointed-top morel is bred using the pointed-top morel breeding method as described in any one of claims 1 to 9, and the pointed-top morel is promoted for cultivation over a large area.

Images