Lactobacillus plantarum IOB602 for improving the protection mechanism of retinal cells under blue light and its application

Abstract

The present invention provides a plant lactobacillus IOB602 for improving the protection mechanism of retinal cells under blue light and its application, which relates to the technical field of microorganisms. Lactiplantibacillus plantarum , the deposit number is CGMCC No.16021. The bacteria increased the activity of retinal epithelial cells, reduced the content of proinflammatory factors IL-6, IL-1β and TNF-α, relieved inflammation, helped to restore the expression of antioxidant enzymes, and effectively improved macular edema by reducing the content of vascular endothelial growth factor; increased the activity of catalase, superoxide dismutase and glutathione peroxidase; reduced the content of proinflammatory factors IL-6, IL-1β and TNF-α in mouse serum, and regulated the body's immune system, thereby enhancing the protection mechanism of retinal cells in a blue light environment.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and specifically relates to Lactobacillus plantarum IOB602 for improving the protection mechanism of retinal cells under blue light and applications thereof. Background Art

[0002] With the development of science and technology and the widespread use of electronic products, people are paying more and more attention to the harm of blue light radiation to the retina. The retinal pigment epithelium is located in the outermost layer of the retina and plays an extremely important physiological role in maintaining the function and structure of photoreceptors. A large amount of evidence shows that continuous blue light exposure can induce apoptosis of retinal pigment epithelial cells, and the accumulation of their own metabolites in retinal pigment epithelial cells can easily induce oxidative stress, leading to impaired photoreceptor function and seriously endangering vision health. The enzyme antioxidant system mainly includes superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT).

[0003] In addition, retinal neovascularization (RNV) is mainly caused by large-area ischemia, hypoxia and microcirculation disorders in the retina, which leads to an increase in angiogenesis stimuli (vascular endothelial growth factor, VEGF) and a simultaneous reduction in the response of angiogenesis inhibitors to local hypoxia, thereby destroying the blood-retinal barrier, causing macular edema, and subsequently causing visual impairment.

[0004] Probiotics refer to living microorganisms that, when administered in adequate amounts, can bring health benefits to the host. Studies have shown that some probiotics and their fermentation products can increase the activity of antioxidant enzymes and protect cells from oxidative stress. In addition, probiotics can also be used to ferment solid fermentation substrates to enhance the antioxidant activity of the fermentation substrates.

[0005] Therefore, it is urgent to find and develop a Lactobacillus plantarum and its microecological preparations that can improve blue light-induced retinal oxidative stress damage. Summary of the invention

[0006] In view of the technical problems existing in the prior art, the present invention aims to provide a Lactobacillus plantarum IOB602 and its application for enhancing the protection mechanism of retinal cells in a blue light environment.

[0007] One of the purposes of the present invention is to provide a plant lactobacillus IOB602 that enhances the protective mechanism of retinal cells under a blue light environment. The plant lactobacillus IOB602 is classified as plant lactobacillus Lactiplantibacillus plantarum It was deposited on June 29, 2018 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No.16021.

[0008] Preferably, the Lactobacillus plantarum IOB602 has at least one of the following functions:

[0009] (1) Improve the vitality of retinal epithelial cells;

[0010] (2) Reduce the levels of cellular pro-inflammatory factors IL-6, IL-1β, and TNF-α;

[0011] (3) Reduce the content of vascular endothelial growth factor and improve macular edema;

[0012] (4) Increase the activity of catalase, superoxide dismutase and glutathione peroxidase;

[0013] (5) Reduce the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α in mouse serum, improve the body's immune imbalance, and regulate the body's immune system.

[0014] Preferably, the Lactobacillus plantarum IOB602 can be used to prepare Lactobacillus plantarum IOB602 bacterial powder and Lactobacillus plantarum IOB602 postbiotic powder.

[0015] Preferably, the preparation method of the Lactobacillus plantarum IOB602 powder and the Lactobacillus plantarum IOB602 postbiotics comprises:

[0016] Inoculate the plant lactobacillus IOB602 in the cryopreserved tube into the slant culture medium and place it at 35-39°C for 18-22 hours of activation time, take the fresh slant bacteria and inoculate it into the seed liquid basic culture medium, and culture it at 35-39°C in a closed state for 16-20 hours; inoculate the cultured seed liquid into the fermentation medium at an inoculation rate of 3±0.5%, and culture it at a temperature of 34-38°C for 16-20 hours;

[0017] After the fermentation is completed, the bacterial liquid is centrifuged to obtain a precipitate, and the precipitate is vacuum freeze-dried to obtain Lactobacillus plantarum IOB602 bacterial powder;

[0018] The bacterial liquid after fermentation is inactivated and dried to obtain Lactobacillus plantarum IOB602 fermentation postbiotics.

[0019] Preferably, the formula of the slant culture medium is: peptone 10.0 g, beef extract 10.0 g, yeast extract 4.5 g, glucose 20.0 g, Tween 80 1.0 mL, dipotassium hydrogen phosphate (7H2O) 2.0 g, sodium acetate (3H2O) 5.0 g, triammonium citrate 2.0 g, magnesium sulfate (7H2O) 0.2 g, manganese sulfate (4H2O) 0.05 g, agar 15.0 g, pH = 6.0 ± 0.2, and the amount of water added is 1 L.

[0020] Preferably, the formula of the seed liquid basic culture medium and the fermentation medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate·7H2O2.0g, sodium acetate·3H2O5.0g, triammonium citrate 2.0g, magnesium sulfate·7H2O0.2g, manganese sulfate·4H2O0.05g, pH=6.0±0.2, and the amount of water added is 1L.

[0021] Preferably, the viable bacterial count of the Lactobacillus plantarum IOB602 powder is 5×10 10 CFU / g-1×10 11 CFU / g.

[0022] Preferably, the Lactobacillus plantarum IOB602 can be used to prepare medicines for enhancing the protective mechanism of retinal cells in a blue light environment.

[0023] Preferably, the Lactobacillus plantarum IOB602 can be used to prepare food or health products for alleviating visual fatigue.

[0024] Beneficial effects of the present invention:

[0025] The present invention provides a Lactobacillus plantarum IOB602 for improving the protection mechanism of retinal cells in a blue light environment. The bacterium improves the vitality of retinal epithelial cells to enhance antioxidant capacity, provide metabolic support, promote cell repair, maintain the blood-retina barrier, reduce cell apoptosis and other mechanisms, thereby improving the protection ability of the retina in a blue light environment; Lactobacillus plantarum IOB602 reduces the content of cellular proinflammatory factors IL-6, IL-1β, and TNF-α, alleviates inflammation, helps maintain the integrity of the blood-retina barrier, prevents harmful substances from penetrating into the retina, reduces damage to visual cells, reduces tissue damage and cell apoptosis risks, enhances the vitality and function of retinal cells, improves their resistance to blue light, and also helps to restore the expression of antioxidant enzymes, enhances the ability of cells to respond to oxidative stress caused by blue light, and reduces the accumulation of reactive oxygen.

[0026] Lactobacillus plantarum IOB602 can effectively improve macular edema by reducing the content of vascular endothelial growth factor (VEGF), thereby enhancing the protection mechanism of retinal cells in a blue light environment. Increased VEGF usually leads to abnormal proliferation and increased permeability of retinal blood vessels, causing macular edema, and then damaging the function of visual cells. By inhibiting the production of VEGF, retinal edema can be reduced, the integrity of the blood-retinal barrier can be maintained, the inflammatory response can be reduced, and the nutrient supply of cells can be improved, thereby enhancing the vitality and resistance of retinal cells, enabling them to better cope with oxidative stress and damage caused by blue light, and promoting the overall health of the retina.

[0027] Lactobacillus plantarum IOB602 can also increase the activity of catalase, superoxide dismutase and glutathione peroxidase; these enzymes play a key role in antioxidant defense, and can effectively remove reactive oxygen species (ROS) and hydrogen peroxide induced by blue light, reducing the damage of oxidative stress to visual cells. Specifically, SOD converts superoxide into oxygen and hydrogen peroxide, catalase decomposes hydrogen peroxide into water and oxygen, and glutathione peroxidase further removes hydrogen peroxide and lipid peroxides by utilizing glutathione. By increasing the activity of these antioxidant enzymes, the antioxidant capacity of retinal cells can be enhanced, cell damage and apoptosis can be reduced, thereby better protecting retinal health. At the same time, Lactobacillus plantarum IOB602 also reduces the content of proinflammatory factors IL-6, IL-1β, and TNF-α in mouse serum, improves the body's immune imbalance, and regulates the body's immune system. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is the activity graph of retinal epithelial cells;

[0029] Figure 2 is the level of IL-6, a pro-inflammatory factor in cells;

[0030] Figure 3 is the level of IL-1β, a pro-inflammatory factor in cells;

[0031] Figure 4 is the level of TNF-α, a pro-inflammatory factor in cells;

[0032] Figure 5 is the vascular endothelial growth factor content of cells;

[0033] Figure 6 is the activity of cell catalase;

[0034] Figure 7 It is the activity of superoxide dismutase in cells;

[0035] Figure 8 is the activity of cellular glutathione peroxidase;

[0036] Fig. 9 is the level of pro-inflammatory factor IL-6 in serum;

[0037] Fig.10 is the level of pro-inflammatory factor IL-1β in serum;

[0038] Fig.11 is the level of pro-inflammatory factor TNF-α in serum;

[0039] Fig.12 is the content of vascular endothelial growth factor in serum;

[0040] Fig.13is the activity of catalase in serum;

[0041] Fig.14 It is the activity of superoxide dismutase in serum;

[0042] Fig.15 is the activity of glutathione peroxidase in serum;

[0043] Fig.16 is the malondialdehyde activity in serum;

[0044] Fig.17 Mouse eye tissue slices.

[0045] The Lactobacillus plantarum IOB602 used in the present invention is classified and named as Lactobacillus plantarum Lactiplantibacillus plantarum It was deposited on June 29, 2018 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No.16021. DETAILED DESCRIPTION

[0046] The present invention provides a plant lactobacillus IOB602 for improving the protection mechanism of retinal cells under a blue light environment, wherein the plant lactobacillus IOB602 is classified and named as plant lactobacillus Lactiplantibacillus plantarum It was deposited on June 29, 2018 in the General Microbiology Center of China Microorganism Culture Collection, referred to as CGMCC. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No.16021.

[0047] In a preferred embodiment of the present invention, the Lactobacillus plantarum IOB602 has at least one of the following functions:

[0048] (1) Improve the vitality of retinal epithelial cells;

[0049] (2) Reduce the levels of cellular pro-inflammatory factors IL-6, IL-1β, and TNF-α;

[0050] (3) Reduce the content of vascular endothelial growth factor and improve macular edema;

[0051] (4) Increase the activity of catalase, superoxide dismutase and glutathione peroxidase;

[0052] (5) Reduce the levels of pro-inflammatory factors IL-6, IL-1β, and TNF-α in mouse serum, improve the body's immune imbalance, and regulate the body's immune system.

[0053] In a preferred embodiment of the present invention, the Lactobacillus plantarum IOB602 can be used to prepare Lactobacillus plantarum IOB602 powder and Lactobacillus plantarum IOB602 postbiotic powder.

[0054] In a preferred embodiment of the present invention, the preparation method of the Lactobacillus plantarum IOB602 powder and the Lactobacillus plantarum IOB602 postbiotics comprises:

[0055] Inoculate the plant lactobacillus IOB602 in the cryopreserved tube into the slant culture medium and place it at 35-39°C for 18-22 hours of activation time, take the fresh slant bacteria and inoculate it into the seed liquid basic culture medium, and culture it at 35-39°C in a closed state for 16-20 hours; inoculate the cultured seed liquid into the fermentation medium at an inoculation rate of 3±0.5%, and culture it at a temperature of 34-38°C for 16-20 hours;

[0056] After the fermentation is completed, the bacterial liquid is centrifuged to obtain a precipitate, and the precipitate is vacuum freeze-dried to obtain Lactobacillus plantarum IOB602 bacterial powder;

[0057] After the fermentation is completed, the bacterial liquid is inactivated and dried to obtain the Lactobacillus plantarum IOB602 fermented raw material powder.

[0058] In a preferred embodiment of the present invention, the formula of the slant culture medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate (7H2O) 2.0g, sodium acetate (3H2O) 5.0g, triammonium citrate 2.0g, magnesium sulfate (7H2O) 0.2g, manganese sulfate (4H2O) 0.05g, agar 15.0g, pH=6.0±0.2, and the amount of water added is 1L.

[0059] In a preferred embodiment of the present invention, the formula of the seed liquid basic culture medium and the fermentation medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate·7H2O2.0g, sodium acetate·3H2O5.0g, triammonium citrate 2.0g, magnesium sulfate·7H2O0.2g, manganese sulfate·4H2O0.05g, pH=6.0±0.2, and the amount of water added is 1L.

[0060] In a preferred embodiment of the present invention, the viable bacterial count of the Lactobacillus plantarum IOB602 powder is 5×10 10 CFU / g-1×10 11 CFU / g.

[0061] In a preferred embodiment of the present invention, the Lactobacillus plantarum IOB602 can be used to prepare medicines for enhancing the protective mechanism of retinal cells in a blue light environment.

[0062] In a preferred embodiment of the present invention, the Lactobacillus plantarum IOB602 can be used to prepare food or health products for alleviating visual fatigue.

[0063] Example 1

[0064] Preparation method of Lactobacillus plantarum IOB602 powder and postbiotics

[0065] 1. Culture medium formula:

[0066] The formula of the slant culture medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate (7H2O) 2.0g, sodium acetate (3H2O) 5.0g, triammonium citrate 2.0g, magnesium sulfate (7H2O) 0.2g, manganese sulfate (4H2O) 0.05g, agar 15.0g, pH=6.0±0.2, and the amount of water added is 1L.

[0067] The formula of seed liquid basic culture medium and fermentation medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate·7H2O2.0g, sodium acetate·3H2O5.0g, triammonium citrate 2.0g, magnesium sulfate·7H2O0.2g, manganese sulfate·4H2O0.05g, pH=6.0±0.2, and the amount of water added is 1L.

[0068] 2. Process

[0069] Inoculate the Lactobacillus plantarum IOB602 in the cryopreservation tube into the slant culture medium and place it at 35-39°C for culture. The activation time is 18-22 hours. After activation, the colonies are milky white, round, moist, opaque, and have neat edges. Microscopically, the bacteria are rod-shaped, arranged singly or in pairs, and are Gram-positive.

[0070] Take fresh slant strains and inoculate them into the seed liquid basic culture medium, and culture them at 35-39℃ in a closed environment for 16-20h. Inoculate the cultured seed liquid into the fermentation medium at an inoculation rate of 3±0.5%, and culture them at 34-38℃ for 16-20h.

[0071] After the fermentation is completed, the bacterial liquid is centrifuged to obtain a precipitate, and the precipitate is vacuum freeze-dried to obtain Lactobacillus plantarum IOB602 bacterial powder;

[0072] The bacterial liquid after fermentation is inactivated and dried to obtain Lactobacillus plantarum IOB602 fermentation postbiotics.

[0073] 3. Activity detection

[0074] The activity of Lactobacillus plantarum IOB602 powder was tested according to the method of "GB4789.35-2024 National Food Safety Standard - Food Microbiology Test - Lactic Acid Bacteria Test". The final test result was 1×10 11 CFU / g.

[0075] Example 2

[0076] Cell experiments:

[0077] 1. Establishment of a cell model of retinal oxidative stress damage induced by blue light

[0078] Human retinal epithelial cells ARPE-19, Pronocell Biotech Co., Ltd.

[0079] Install a blue LED lamp with a wavelength of 430 nm in the incubator and adjust it to a suitable height so that the light intensity reaching the surface of the cell culture plate is between 2300 and 2700 Lux. Adjust the culture temperature to 37°C. Incubate ARPE-19 cells in good growth state at a rate of 5×10 5 The cells were inoculated into a culture plate containing cell growth medium at a concentration of 100 cells / mL. When the cell density reached 65-75%, the cells were pretreated with cell maintenance medium for 6 h, and then exposed to blue light for 12 h.

[0080] Table 1 Cell experimental groups and treatments

[0081]

[0082] 2. Lactobacillus plantarum IOB602 improves the vitality of retinal epithelial cells

[0083] ARPE-19 cells with good growth status were cultured at 5×10 5 The cells were inoculated into a culture plate containing cell growth medium at a concentration of 100 μL / mL. After the APRE-19 cells with a growth density of 65-75% were evenly blown, the cell suspension was added to a 96-well cell culture plate at a volume of 100 μL per well and gently shaken. The normal group was added with cell maintenance medium and cultured for 12 hours, then covered with black tape to prevent light exposure and cultured for 12 hours; the blue light exposure group was cultured with cell maintenance medium for 12 hours and exposed to blue light for 12 hours; the experimental group contained 10 8 After pretreatment with IOB602 cell maintenance medium at 100 CFU / mL for 12 h, the cells were exposed to blue light for 12 h.

[0084] Add 20 μL of MTT reagent (5.0 mg / mL) to each well in the dark and incubate in an incubator for 3-4 h. Carefully discard the supernatant, add 150 μL of DMSO to each well, shake thoroughly until the formazan purple crystals are completely dissolved, and measure the absorbance at 490 nm. Calculate the percentage of the absorbance of the test group cells / the absorbance of the normal cells.

[0085] The experimental results are as follows Figure 1 As shown, the survival rate of APRE-19 cells decreased after blue light pretreatment. Compared with the blue light exposure group, the survival rate of cells was increased after intervention with cell maintenance medium containing IOB602 bacterial solution.

[0086] 3. Lactobacillus plantarum IOB602 reduces the levels of pro-inflammatory factors IL-6, IL-1β and TNF-α

[0087] Experimental Materials:

[0088] IL-6, IL-1β, TNF-α ELISA kits, manufacturer: Beijing Sizhengbai Biotechnology Co., Ltd.

[0089] According to the instructions of the ELISA kit, the supernatant was used to detect the expression of inflammatory factors. The test results were as follows: Figure 2 , Figure 3 and Figure 4 shown.

[0090] IL-6, TNF-α, and IL-1β, as inflammatory cytokines, have strong pro-inflammatory activity and can stimulate the expression of multiple pro-inflammatory mediators, exacerbating the inflammatory response. Compared with the normal control group, the levels of IL-6, TNF-α, and IL-1β in the blue light exposure group increased significantly, by 62.96%, 37.79%, and 32.89%, respectively. Compared with the blue light exposure group, after IOB602 pretreatment, the levels of IL-6, TNF-α, and IL-1β decreased, by 30.30%, 18.00%, and 26.26%, respectively.

[0091] 4. Lactobacillus plantarum IOB602 reduces the content of vascular endothelial growth factor in cells

[0092] Experimental Materials:

[0093] VEGF ELISA kit was purchased from Beijing Sizhengbai Biotechnology Co., Ltd.

[0094] According to the instructions of the ELISA kit, the supernatant was used to detect the expression of vascular endothelial growth factor. The test results were as follows: Figure 5 shown.

[0095] Overexpression of vascular endothelial growth factor (VEGF) in retinal cells can lead to vascular leakage, the formation of new choroidal blood vessels and neuroretinal degeneration, thus causing macular enlargement; at the same time, as an important inflammatory cell chemokine, it can also promote the expression of other cytokines and aggravate retinal inflammatory response. Compared with the normal control group, the VEGF level in the blue light exposure group increased significantly by 27.53%, while compared with the blue light exposure group, after IOB602 pretreatment, the VEGF level was greatly reduced by 20.39%, which was basically the same as the normal control group.

[0096] 5. Lactobacillus plantarum IOB602 improves the activity of cellular catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)

[0097] Experimental materials: CAT, SOD and GSH-Px kits were purchased from Nanjing Jiancheng Bioengineering Institute.

[0098] Human retinal epithelial cells ARPE-19 were ultrasonically disrupted under ice bath conditions at 300 W power, 5 s / time, 30 s interval, and repeated 10 times. Cells were collected and antioxidant enzyme activities were determined. The specific experimental operations were completed according to CAT, SOD and GSH-Px kits.

[0099] Catalase is one of the key enzymes in the endogenous antioxidant system. Its main function is to accelerate the decomposition of hydrogen peroxide, reduce the generation of harmful free radicals, and remove peroxides in the body, thereby protecting cells. Superoxide dismutase, as an important antioxidant enzyme that can convert catalytic free radicals (ROS) into non-toxic and harmless substances, can prevent ROS-induced oxidative stress. By cooperating with SOD and CAT, it accelerates the decomposition of hydrogen peroxide and reduces the generation of hydroxyl free radicals, removes endogenous ROS, and protects the integrity of the cell membrane structure and function. Figure 6 , Figure 7 and Figure 8 As shown, compared with the blank control group, the activities of CAT, SOD and GSH-Px in the blue light exposure group were significantly decreased, by 64.06%, 48.21% and 73.63%, respectively, which indicates that blue light irradiation will destroy the endogenous antioxidant system of cells and lead to cellular oxidative stress; compared with the blue light exposure group, the activities of antioxidant enzymes CAT, SOD and GSH-Px in ARPE-19 cells were significantly increased after IOB602 pretreatment, by 106.10%, 37.50% and 148.34%, respectively.

[0100] Example 2

[0101] Animal experiments

[0102] 1. Animal Model Establishment

[0103] 64 specific pathogen-free (SPF) female BALB / c mice, 18-20 g, were raised in the animal laboratory of Tianjin University of Science and Technology, with a 12-h light-dark cycle and standard temperature and humidity (22 ± 3°C and 30-60%). During the experiment, the mice moved freely, ate and drank water normally, and the bedding and drinking water were changed every day. The BALB / c mice were randomly divided into 5 groups according to the experimental groups, with 8 mice in each group. The mice were given the corresponding samples by gavage for 4 consecutive weeks. The specific mouse groups and samples are shown in Table 2:

[0104] Table 2 Mouse groups and samples

[0105]

[0106] After 12 h of dark adaptation, the mice were exposed to 4500-5500 Lux blue light for 12 h in an exposure box maintained at 23 ± 2°C for 7 consecutive days. During the light exposure period, the mice ate and drank normally.

[0107] After the illumination, the mice were kept under dim circulating light. After the illumination ended, blood was collected, the eyeballs were separated, and one part of the eyeballs was stored in eyeball fixative for pathological sectioning, and the other part, the retina was quickly separated on ice and transferred to liquid nitrogen for storage for subsequent experiments.

[0108] 2. Lactobacillus plantarum IOB602 reduces the level of inflammatory factors in serum

[0109] After the mouse plasma was separated and placed at 4°C for 12 h, it was centrifuged at 12,000 rpm for 15 min, and the upper serum was collected. The IL-6, IL-1β, and TNF-α ELISA kits were used to determine the content of IL-6, IL-1β, and TNF-α inflammatory factors.

[0110] The measurement results are as follows Fig. 9 , Fig.10 and Fig.11 As shown in the figure, compared with the blank group, the levels of IL-6, IL-1β, and TNF-α in the serum of the mice in the model group were significantly increased, by 62.52%, 56.46%, and 21.63%, respectively; compared with the model group, the levels of IL-1β and TNF-α in the IOB602 live bacteria group were reduced to a certain extent, by 4.90% and 5.10%, respectively.

[0111] The levels of IL-6, IL-1β, and TNF-α in the IOB602 postbiotic group were all reduced to a certain extent, by 8.63%, 7.87%, and 7.37%, respectively. The improvement effect of postbiotics was more obvious.

[0112] 3. Lactobacillus plantarum IOB602 reduces the level of vascular endothelial growth factor in serum

[0113] After the mouse plasma was separated and placed at 4°C for 12 h, it was centrifuged at 12,000 rpm for 15 min, and the upper serum was collected. The VEGF ELISA kit was used to determine the vascular endothelial growth factor content.

[0114] The results are as follows Fig.12 As shown, compared with the blank group, the vascular endothelial growth factor content in the serum of mice in the model group was significantly increased, by 29.83%; compared with the model group, the vascular endothelial growth factor content in the IOB602 live bacteria group and the postbiotic group was reduced to a certain extent, by 4.59% and 11.17% respectively, and the improvement effect of postbiotics was more obvious.

[0115] 4. Lactobacillus plantarum IOB602 increases the content of antioxidant enzymes in serum

[0116] The separated mouse retinal tissue was added to PBS at a volume ratio of 1:9, and the tissue was ground into a 10% retinal tissue homogenate under ice bath conditions. Centrifuged at 4°C, 3000 rpm for 15 min. The supernatant was collected and the protein concentration was determined, and then measured using CAT, SOD, GSH-Px and MDA kits.

[0117] The experimental results are as follows Fig.13 , Fig.14 , Fig.15 and Fig.16 As shown, compared with the blank group, the levels of catalase, superoxide dismutase and glutathione peroxidase in the retina of mice in the model group were significantly decreased, which were decreased by 40.45%, 44.81% and 29.48%, respectively, and the content of malondialdehyde (MDA) was significantly increased, which was increased by 26.21%; compared with the model group, the levels of catalase, superoxide dismutase and glutathione peroxidase in the IOB602 live bacteria group were increased to a certain extent, which were increased by 8.29%, 19.40% and 10.63%, respectively, and the content of malondialdehyde was decreased to a certain extent, which was decreased by 7.64%. The levels of catalase, superoxide dismutase and glutathione peroxidase in the IOB602 postbiotic group were increased to a certain extent, which were increased by 30.76%, 54.04% and 35.81%, respectively, and the content of malondialdehyde was decreased to a certain extent, which was decreased by 13.86%. The improvement effect of the postbiotic group was more obvious.

[0118] 5. HE Staining

[0119] The eyeball tissue was fully immersed in the eyeball fixative for 24 hours, and then embedded in paraffin and sliced. The thickness of the paraffin sections was 4 μm. After drying at room temperature, they were stained with hematoxylin and eosin (H&E). The changes in the mouse retinal structure were observed under a microscope and photographs were recorded.

[0120] As a photosensitive layer, the retina mainly transmits visual information from the outside world to the visual center of the brain through the optic nerve. The neural retina consists of nine layers, including the ganglion cell layer, inner nuclear layer, outer nuclear layer, and photoreceptor layer. Retinal damage was evaluated by image analysis of H&E-stained retinal tissue. Representative images of retinal sections of each group are shown in Figure 1. Fig.17 As shown. In the blank group, H&E staining results showed that the retinal layers were intact, the cells were tightly arranged, the morphology was normal, the number of pigment granules in the retinal pigment epithelial cells was large, and the chromatin density in the nucleus was high. However, the retinal histological changes in the model group mice were obvious, the cells were scattered, the intercellular gaps became larger, the chromatin in the nucleus agglomerated, and the number of cell layers decreased. The administration of IOB602 live bacteria and postbiotics improved the overall morphology of the retina to a certain extent, thereby protecting the retina from blue light damage.

Claims

1. A Lactobacillus plantarum ( Lactiplantibacillus plantarum ) The use of IOB602 in the preparation of a drug for improving blue light-induced retinal oxidative stress damage, characterized in that: The deposit number of the Lactobacillus plantarum IOB602 is CGMCC No.16021.

2. The use according to claim 1, characterized in that The medicine for improving blue light-induced retinal oxidative stress damage comprises Lactobacillus plantarum IOB602 bacterial powder and / or Lactobacillus plantarum IOB602 postbiotics; The preparation method of the Lactobacillus plantarum IOB602 powder and the Lactobacillus plantarum IOB602 postbiotics comprises: Inoculate the plant lactobacillus IOB602 in the cryopreserved tube into the slant culture medium and place it at 35-39°C for 18-22 hours of activation time, take the fresh slant bacteria and inoculate it into the seed liquid basic culture medium, and culture it at 35-39°C in a closed state for 16-20 hours; inoculate the cultured seed liquid into the fermentation medium at an inoculation rate of 3±0.5%, and culture it at a temperature of 34-38°C for 16-20 hours; After the fermentation is completed, the bacterial liquid is centrifuged to obtain a precipitate, and the precipitate is vacuum freeze-dried to obtain Lactobacillus plantarum IOB602 bacterial powder; The bacterial liquid after fermentation is inactivated and dried to obtain Lactobacillus plantarum IOB602 fermentation postbiotics.

3. The use according to claim 2, characterized in that The formula of the slant culture medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate·7H2O2.0g, sodium acetate·3H2O5.0g, triammonium citrate 2.0g, magnesium sulfate·7H2O0.2g, manganese sulfate·4H2O0.05g, agar 15.0g, pH=6.0±0.2, and the amount of water added is 1L.

4. The use according to claim 2, characterized in that The formula of seed liquid basic culture medium and fermentation medium is: peptone 10.0g, beef extract 10.0g, yeast extract 4.5g, glucose 20.0g, Tween 80 1.0mL, dipotassium hydrogen phosphate·7H2O2.0g, sodium acetate·3H2O5.0g, triammonium citrate 2.0g, magnesium sulfate·7H2O0.2g, manganese sulfate·4H2O0.05g, pH=6.0±0.2, and the amount of water added is 1L.

5. The use according to claim 2, characterized in that The viable bacterial count of the Lactobacillus plantarum IOB602 powder is 5×10 10 CFU / g-1×10 11 CFU / g.

6. A plant lactobacillus ( Lactiplantibacillus plantarum ) The application of IOB602 in preparing a health food for relieving visual fatigue is characterized in that: The deposit number of the Lactobacillus plantarum IOB602 is CGMCC No.16021.

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