Preparation method of traditional Chinese medicine ointment for treating rhinitis

Abstract

The invention discloses a preparation method of a traditional Chinese medicine ointment for treating rhinitis, which comprises the following steps: crushing a plurality of traditional Chinese medicine raw materials according to a certain ratio, mixing with a snail liquid extract and an earthworm tissue extract, adding a plurality of enzymes such as cellulase, pectinase and papain for enzymolysis, adding a fermentation substrate, and inoculating a strain for fermentation; the preparation method comprises the following steps: fermenting, decocting for many times by a traditional Chinese medicine decocting machine, concentrating by a decoction process to obtain a thick traditional Chinese medicine paste semi-finished product, cooling the traditional Chinese medicine paste semi-finished product, adding lard paste, beeswax and related auxiliary materials, and carrying out ultraviolet disinfection and canning to obtain a finished product. According to the preparation method of the traditional Chinese medicine ointment for treating rhinitis, through special process treatment, the content of effective components in the medicine is fully increased, the absorption effect of nasal mucosa on the effective components is promoted, and the irritation of the nasal mucosa on the nasal cavity is reduced; meanwhile, by adding auxiliary materials, the absorption effect and the treatment effect after the medicine is smeared on the nasal cavity are further improved.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, and in particular to a method for preparing a traditional Chinese medicine paste for treating rhinitis. Background Art

[0002] Rhinitis is an inflammation of the nasal cavity caused by viruses, bacteria, allergens (such as pollen), various physical and chemical factors (such as irritating gases) and certain systemic diseases. The main pathological changes are congestion, swelling, exudation, hyperplasia, atrophy or necrosis of the nasal mucosa. Clinically, it can be divided into acute rhinitis and chronic rhinitis according to the speed of onset. Among them, chronic rhinitis can be divided into allergic rhinitis (allergic rhinitis) and non-allergic rhinitis. Patients with rhinitis are mainly characterized by symptoms such as runny nose, nasal congestion, sneezing, and nasal itching. Chronic rhinitis often recurs, which may cause certain difficulties in the patient's life. For example, patients with severe nasal congestion often have difficulty falling asleep. Some patients may develop complications such as acute sinusitis, acute otitis media, and asthma. Some patients may also suffer from certain psychological effects due to the long-term presence of rhinitis symptoms. At present, the commonly used treatment options in Western medicine are mainly drug therapy and surgical treatment. Drug therapy is mainly based on antibiotics, which can easily cause drug-induced rhinitis, with a low cure rate and a high recurrence rate. Surgical treatment costs a lot of time and money. If the disease recurs after surgery, it will repeatedly cause psychological and physical pain to the patient. However, traditional Chinese medicine adheres to the principle of treating the symptoms and strengthening the root cause. It uses traditional Chinese medicine to remove the lesions and prevent them from recurring.

[0003] At present, whether it is Western medicine or traditional Chinese medicine, the most common method of drug treatment is nasal administration. Western medicine's antihistamine and hormone sprays are prone to drug dependence, and nasal symptoms worsen after stopping the drug. The use of such drugs will also cause side effects such as nose bleeding, local irritation, and bloody secretions. Due to the above situation, Western medicine treatment is often not used for a long time. The effect is limited and the operation is inconvenient, time-consuming and laborious, resulting in the current low cure rate and a high recurrence rate, which repeatedly causes psychological and physical pain to patients. Traditional Chinese medicine drugs are less irritating, but the course of treatment is longer, the efficacy is slightly worse, and the efficacy is mostly unclear. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing a traditional Chinese medicine paste for treating rhinitis. Through special process treatment, the content of active ingredients in the medicine is fully improved, the absorption effect of the active ingredients by the nasal mucosa is promoted, and the irritation to the nasal cavity is reduced. At the same time, the absorption effect and therapeutic effect of the medicine after being applied to the nasal cavity are further improved by adding auxiliary materials.

[0005] In order to achieve the above object, the solution of the present invention is: A method for preparing a Chinese medicine paste for treating rhinitis comprises the following steps: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2-3 parts of asarum, 2-3 parts of wormwood, 2-3 parts of mint, 8-9 parts of plantain, 6-7 parts of honeysuckle, 12-13 parts of angelica, 6-7 parts of ligusticum, 15-16 parts of magnolia, 13-14 parts of xanthium, 7-8 parts of schizonepeta, 12-13 parts of forsythia, 11-12 parts of scutellaria, 4-5 parts of panax notoginseng, 8-9 parts of siler, 7-8 parts of notopterygium, 3-4 parts of dandelion, 2-3 parts of vitex rotundus, 15-16 parts of scutellaria, 3-4 parts of erlang sword, and 3-4 parts of sophora flavescens; Step 2, then mixing the above-mentioned Chinese medicine powders in proportion, mixing and stirring with distilled water at a mass ratio of 1:1.2-1.3, and then adding snail liquid extract and earthworm tissue extract, and then adding cellulase and pectinase in sequence for enzymolysis, and adding papain for enzymolysis, and obtaining a Chinese medicine treatment liquid after enzymolysis; Step 3, then adding the fermentation substrate to the Chinese medicinal material processing liquid, stirring evenly, inoculating Bacillus, Lactobacillus plantarum, Eurotium cristatum and Lactobacillus acidophilus, standing and fermenting at room temperature, and obtaining the Chinese medicinal material fermentation liquid after fermenting for 26 to 32 hours; The fermentation substrate includes the following raw materials: 4.5% to 5.0% glucose, 1.8% to 2.1% maltodextrin, 0.09% to 0.13% yeast extract powder, and 0.45% to 0.52% sodium acetate. The amount of the raw materials of the fermentation substrate is calculated as a percentage of the mass of the traditional Chinese medicine processing liquid; Step 4, then the obtained Chinese medicine fermentation liquid and the pig pancreas filtrate are mixed in a mass ratio of 1:0.9-1.1, stirred evenly and poured into a Chinese medicine decoction machine, after multiple decoctions, all the obtained filtrates are mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and a Chinese medicine paste semi-finished product is obtained; Step 5, then wait for the Chinese medicine paste semi-finished product to cool to 80-85° C., mix the Chinese medicine paste semi-finished product, lard cream and beeswax in a mass ratio of 1:0.3-0.5:0.2-0.6, and stir evenly, wait until it is completely cooled to room temperature, add auxiliary materials and stir evenly again, and finally put it into a product container after ultraviolet disinfection to obtain a finished Chinese medicine paste; The auxiliary materials include the following raw materials: 1.0%-1.5% glycerol, 0.5%-1.5% garlic juice, 0.2%-0.5% sesame oil and 0.05%-0.2% chitosan, and the amount of the auxiliary materials is calculated as a percentage of the mass of the semi-finished Chinese medicine paste.

[0006] In step 2, the mass ratio of the snail liquid extract and the earthworm tissue extract is 1:1-2, and the added amounts of the snail liquid extract and the earthworm tissue extract account for 0.12%-0.36% of the sum of the amounts of each Chinese medicine powder.

[0007] In step 2, the enzymatic activity of cellulase is 10000 U / g, the enzymatic activity of pectinase is 10000 U / g, and the enzymatic hydrolysis conditions of cellulase and pectinase are as follows: the amount of cellulase added accounts for 0.21%~0.36% of the sum of the amounts of each Chinese medicine powder, the amount of pectinase added accounts for 0.19%~0.31% of the sum of the amounts of each Chinese medicine powder, the enzymatic hydrolysis temperature is 40~45°C, the pH is adjusted to 4.8~5.3, and the enzymatic hydrolysis time is 55~60 min.

[0008] In step 2, the enzymatic activity of papain is: 10000 U / g, and the enzymatic hydrolysis conditions of papain are: the added amount accounts for 0.12%~0.13% of the sum of the amounts of each Chinese medicine powder, the enzymatic hydrolysis temperature is 42~60°C, the pH is adjusted to 4.5~6.0, and the enzymatic hydrolysis time is 40~55 min.

[0009] In step 3, the inoculation ratios of Bacillus, Lactobacillus plantarum, Eurotium cristatum and Lactobacillus acidophilus are respectively 0.70% to 0.75% of the mass amount of the traditional Chinese medicine processing liquid.

[0010] In step 4, the preparation method of the porcine pancreas filtrate is: mixing the porcine pancreas and distilled water in a mass ratio of 1:1.4-1.5, boiling and filtering through absorbent cotton gauze, and the obtained filtrate is the porcine pancreas filtrate.

[0011] In step 4, the decoction process is a three-step decoction process, wherein: The first decoction process is: decoction at high heat for 29-33 min, and the first filtrate and the first residue are obtained after filtering through absorbent cotton gauze; the second decoction process is: mix the first residue with distilled water in a mass ratio of 1:1.5-1.6, soak for 50-60 min, boil over high heat, and then decoct over low heat for 80-90 min, and filter through absorbent cotton gauze to obtain the second filtrate and the second residue; the third decoction process is: mix the second residue with distilled water in a mass ratio of 1:1-1.2, soak for 120-130 min, decoct over high heat for 50-60 min, and filter through absorbent cotton gauze to obtain the third filtrate and the third residue.

[0012] In step 5, the process of ultraviolet disinfection is: using an ultraviolet lamp with a wavelength of 253.7 nm to irradiate the material at a distance of 200-300 mm for 50-60 min.

[0013] After adopting the above technical scheme, the preparation method of a Chinese medicine paste for treating rhinitis of the present invention comprises the following steps: firstly, a plurality of Chinese medicine raw materials are crushed according to a certain ratio, and then mixed with snail liquid extract and earthworm tissue extract, and a plurality of enzymes such as cellulase, pectinase and papain are added for enzymolysis, and the utilization efficiency of the Chinese medicine raw materials is improved by enzymolysis, and the initial effect during subsequent fermentation is improved; then, a fermentation substrate is added and the strain is inoculated for fermentation, and the macromolecular effective components in the Chinese medicine are decomposed and converted into easily absorbable small molecules by the action of fermentation, which is more conducive to the absorption of the drug, and at the same time, the metabolism of microorganisms can also decompose the harmful components in the Chinese medicine and reduce the irritation to the nasal cavity; after fermentation, the Chinese medicine is decocted for multiple times by a Chinese medicine decoction machine, and the viscous Chinese medicine paste semi-finished product is obtained by decoction process, and lard cream, beeswax and related auxiliary materials are added after the Chinese medicine paste semi-finished product is cooled, and the finished product is obtained after ultraviolet disinfection and canning, and each step has the following characteristics: 1. The Chinese medicine powder of the present invention comprises the following raw materials in parts by weight: 2-3 parts of asarum, 2-3 parts of wormwood, 2-3 parts of mint, 8-9 parts of plantain, 6-7 parts of honeysuckle, 12-13 parts of angelica dahurica, 6-7 parts of ligusticum, 15-16 parts of magnolia, 13-14 parts of xanthium, 7-8 parts of schizonepeta, 12-13 parts of forsythia, 11-12 parts of scutellaria, 4-5 parts of notoginseng, 8-9 parts of siler, 7-8 parts of notopterygium, 3-4 parts of dandelion, 2-3 parts of vitex rotundus, 15-16 parts of scutellaria baicalensis, 3-4 parts of erlangjian, and 3-4 parts of sophora flavescens; wherein: ① The main medicine is: Xinyi, which is pungent and warm in nature, belongs to the stomach meridian, has the effects of dispersing wind and cold, clearing the nasal passages, can shrink the nasal mucosal blood vessels, promote the production of protein coagulation on the nasal mucosal tissue, protect the nasal mucosa and reduce the production of nasal mucosal secretions; Gebushicao, which is pungent and warm in nature, belongs to the lung, liver and spleen meridians, has the effects of dispersing wind and cold, relieving cough and reducing phlegm, killing bacteria, clearing the nasal passages, and relieving itching. They are both main medicines and can quickly relieve rhinitis symptoms such as nasal congestion, runny nose, and nasal itching; ② The ministerial drugs are: Angelica dahurica, which is pungent in taste and warm in nature, enters the lung and stomach meridians, has anti-inflammatory and antibacterial effects, and has inhibitory effects on Escherichia coli, Shigella dysenteriae, Proteus, Salmonella typhi, Salmonella paratyphi, Pseudomonas aeruginosa, Vibrio cholerae, and Mycobacterium tuberculosis. Xanthium sibiricum is pungent, bitter, warm in nature, dispels rheumatism, clears the nasal passages, relieves pain and kills insects, has the effects of dispelling wind, dissipating heat, removing dampness, and detoxifying, has a certain inhibitory effect on Staphylococcus aureus, Group B Streptococcus, and Pneumococcus, and has antifungal effects. Forsythia suspensa is bitter in nature and cool in nature, enters the heart, liver, and gallbladder meridians, has the effects of clearing away heat and detoxifying, evacuating wind-heat, and reducing swelling and dispersing knots. Scutellaria baicalensis is bitter in taste and cold in nature, enters the lung, gallbladder, spleen, large intestine, and small intestine meridians, and its main effects are purging real fire, removing dampness and heat, stopping bleeding and stagnating the fetus. These four herbs, as ministerial drugs, have the effects of warming and tonifying the kidney and lung, regulating the internal organs, and reducing inflammation, inhibiting bacteria, and detoxifying. ③ The adjuvants are: Di Ding, which is cold in nature, bitter in taste, and enters the heart and spleen meridians. The effects and functions of Di Ding include clearing away heat and removing fire, anti-inflammatory and sterilizing, reducing swelling and relieving pain, dissolving suppuration and dispersing nodules, and stopping bleeding and dispersing blood stasis; Flos Lonicerae, which is cold in nature, sweet in taste, and enters the lung, heart, and stomach meridians. It has the effects of clearing away heat and detoxifying, anti-inflammatory, tonifying deficiency and curing wind. It is mainly used to treat bloating, fever due to febrile diseases, carbuncle due to heat toxins, and tumors; Ligusticum chuanxiong, which is pungent and warm in nature, belongs to the bladder meridian. Ligusticum chuanxiong mainly contains volatile oils, cnidium monnieri and other ingredients, which have the effects of sedation, analgesia, antipyretic, anti-inflammatory, blood pressure reduction, coronary artery dilation, increased coronary blood flow, improved myocardial ischemia, and increased hypoxia tolerance; Schizonepeta, which is pungent, slightly bitter, and slightly warm in nature. The benzene contained in Schizonepeta Propylene furan derivatives have good anti-inflammatory and anti-analgesic effects, and Schizonepeta can shorten the time of coagulation and bleeding, and has good hemostatic effects; Saposhnikovia divaricata, pungent and sweet, slightly warm in nature, can promote blood circulation and relieve pain, dispel wind and dampness, etc. According to pharmacological studies, Saposhnikovia divaricata has antipyretic, analgesic, sedative, antibacterial, anti-inflammatory, anticonvulsant, antihistamine, antiviral, anticoagulant, and immunity-enhancing effects; Notopterygium incisum, pungent and bitter, warm in nature, can relieve the cold, dispel wind and dampness, and relieve pain. These six herbs, as ministerial drugs, take into account the characteristics of cold pathogens that are easy to accumulate and become toxic and that they often contain turbid air, can enhance the anti-inflammatory and antibacterial properties of plasters, and have good effects of strengthening the body and dispelling pathogens, clearing away heat and detoxifying, clearing the orifices and reducing swelling, and reducing swelling and relieving pain; ④ Envoys: Peppermint: pungent, cool in nature, enters the lung and liver meridians, dispels wind-heat, clears the head, relieves sore throat and rashes, soothes the liver and promotes qi circulation; Panax notoginseng: sweet, bitter, warm in nature, removes blood stasis and stops bleeding, promotes blood circulation and relieves pain, can promote the proliferation of multifunctional hematopoietic stem cells for nasal mucosal bleeding caused by rhinitis, and has a hematopoietic effect; Dandelion: bitter and sweet in nature, cold, enters the liver and stomach meridians, has the effects of clearing heat and detoxifying, reducing swelling, and promoting urination, has antibacterial effects, can stimulate the body's immune function, and achieve the effects of gallbladder and liver protection; Vitex rotundifolia: pungent, bitter, slightly cold in nature, enters the lung, bladder, and liver meridians, dispels wind-heat, clears the head, removes dampness from joints; Erlangjian tastes bitter and sour, and is cool in nature. It has the effects of dispersing blood stasis, clearing heat, relieving cough and relieving asthma; Sophora flavescens tastes bitter and cold, and enters the lung and stomach meridians, which is beneficial for clearing away heat and detoxifying, relieving sore throat and swelling; Asarum is pungent and warm, and enters the heart, lung and kidney meridians, and can relieve exterior symptoms and dispel cold, dispel wind and relieve pain, open the orifices, warm the lungs and transform fluid, and is used for wind-cold colds, headaches, toothaches, nasal congestion and runny nose, allergic rhinitis, rheumatic arthralgia, phlegm and cough; Artemisia argyi is bitter, pungent and warm in nature, and enters the spleen, liver and kidney meridians, and has the effects of removing dampness, dispersing cold, stopping bleeding, reducing inflammation and anti-allergic. The above eight medicines are used as adjuvants to improve the effects of the aforementioned medicines, reconcile deficiencies, and eliminate drug side effects.

[0014] Traditional Chinese medicine believes that the nose is a clear orifice, and is the gateway for breathing and smell. The breathing and olfactory functions of the nose depend on the clear and yang qi produced by the internal viscera and organs, which reaches the nose through the meridians. For example, the nose is closely related to the lungs, and the nose is the external orifice of the lungs. Therefore, when the wind and cold evil qi invade the lung defense, symptoms such as nasal congestion and runny nose can usually occur. In addition, traditional Chinese medicine also believes that the nose is closely related to the spleen. The spleen is the foundation of the acquired constitution, which can transform qi and blood. If the spleen is deficient, symptoms such as runny nose, nasal congestion, and decreased sense of smell can occur. "The main disease is called the monarch, the assistant monarch is called the minister, and the minister is called the messenger." The raw materials of the Chinese medicine prescription in the invention are proportioned according to the principle of monarch, minister, assistant, and messenger. The whole prescription has the effects of clearing away heat and detoxification, warming and nourishing the lungs and kidneys, dispersing cold and opening the orifices, nourishing the lungs and spleen and removing dampness, ventilating and activating blood circulation, opening the orifices, and anti-inflammatory and antibacterial. The drug regulates the lungs, spleen, kidneys and other internal organs to solve the symptoms of rhinitis from the root, so that the curative effect is consolidated and long-term.

[0015] 2. Add snail liquid extract and earthworm extract to the Chinese medicine powder. Snail liquid extract refers to the mucus secreted by snails during crawling. The essence extracted from the mucus is called "snail mucus extract". The functions of snail mucus include moisturizing, reducing redness and swelling, and reducing inflammation and analgesia. Continuous use will make the skin surface smooth and translucent. Various components in earthworm extract, such as lumbrokinase, antimicrobial peptides, lysozyme, plasmin, protease and other ingredients have anticoagulant, thrombolytic, antiviral, antibacterial, antitumor, and promotion of damaged tissue repair. There are many fibrinolytic enzymes in earthworm extracts, collectively referred to as lumbrokinase. Lumbrokinase has high medicinal value. Once absorbed by the body, it can activate plasmin to inhibit platelet aggregation, improve microcirculation and promote thrombus dissolution. Moreover, this substance can also release endorphins and other substances with analgesic effects, so that a good analgesic effect can be achieved. It can also decompose proteins in the inflammatory site, thereby playing an anti-inflammatory effect. In addition to kinase, it also contains a large number of active ingredients such as antibacterial, anti-inflammatory, analgesic, and wound healing. When applied to the skin, it can promote the repair of tissue cells, enhance the physical barrier function of the skin, and relieve itching and pain, as well as repair the skin. At the same time, it can activate immunity and, combined with its own antibacterial ability, can effectively inhibit the reproduction of bacteria and fungi.

[0016] 3. The pretreatment enzymes in the present invention include cellulase, pectinase and papain. Among them, cellulase and pectinase are mainly used to decompose the cell wall of Chinese medicine powder. The cell wall of Chinese medicine is mainly composed of cellulose, hemicellulose, pectin and lignin. By decomposing the cell wall, the effective ingredients in the cell can be effectively released, which is beneficial to the subsequent fermentation and utilization, and is also beneficial to improving the absorption effect during subsequent use; papain is an unpurified multi-enzyme system extracted from the immature fruit of papaya, which has high protease and lipase activities, can hydrolyze macromolecular proteins and part of fat, and decompose them into amino acids and fatty acids that are easily absorbed and utilized.

[0017] 4. The fermentation substrate in the fermentation process of the present invention is composed of glucose, maltodextrin, yeast extract powder and sodium acetate, wherein glucose and maltodextrin mainly provide quick-acting carbon sources, and both glucose and maltodextrin are excellent cosolvents, which can effectively improve the dissolution effect of the solution; yeast extract powder is a biological culture medium product made from high-protein baker's yeast or brewer's yeast, which is rich in nutrients such as protein, amino acids, polypeptides, vitamins and trace elements, and can not only provide a nitrogen source, but also provide a large amount of growth-promoting factors for microorganisms to use; sodium acetate can also be used as a carbon source for microorganisms, but its most important effect is that it can inhibit the growth and reproduction of a large part of bacteria other than lactic acid bacteria, which can effectively improve the stability of fermentation.

[0018] 5. The fermentation bacteria in the fermentation process of the present invention are composed of Bacillus, Lactobacillus plantarum, Eurotium cristatum and Lactobacillus acidophilus, wherein Bacillus is a species of the genus Bacillus, is Gram-positive, is a type of aerobic bacteria, is non-toxic, and can secrete a variety of enzymes such as proteases and antibiotics, Lactobacillus plantarum is a type of lactic acid bacteria, Eurotium cristatum, also known as golden flower, is a fungus belonging to the genus Eurotium of the family Trichodermaceae, and Lactobacillus acidophilus belongs to the genus Lactobacillus, a Gram-positive rod with a rounded end of the rod, and the fermentation process can produce a large amount of enzymes that can decompose the original macromolecular effective ingredients of the traditional Chinese medicine into small molecular components that are easily absorbed through the skin, and at the same time through its metabolic effect.

[0019] 6. The semi-finished Chinese medicine paste of the present invention is mixed with lard cream, garlic juice, chitosan, glycerin and sesame oil. The lard cream has the effect of smoothing the skin and moisturizing and locking moisture; the allicin in garlic has the benefit of sterilization and anti-inflammatory. Allicin can play the role of antibacterial and anti-inflammatory when entering the human body, can effectively inhibit the growth of bacteria and viruses in the body, can play the role of enhancing immunity, and at the same time, allicin can also play the role of sedation and analgesia; chitosan has a broad-spectrum antibacterial effect, can inhibit the growth of bacteria and fungi, and has an anti-inflammatory effect to promote wound healing. Chitosan can promote the regeneration of microvessels in skin wound tissue, improve blood circulation, and accelerate the healing of wounds. Healing, at the same time, it also has the functions of hemostasis, analgesia, and inhibition of scar hyperplasia. Chitosan can increase the body's pH value and enhance the activity of the immune system; glycerol plays a certain moisturizing role, and at the same time it is a good solvent and co-surfactant. By reducing the oil-water interfacial tension, it can fuse and mix the effective ingredients in garlic juice and chitosan with the traditional Chinese medicine paste, improve the uniformity of the paste, and improve the durability of the penetration-promoting effect of garlic juice and chitosan ingredients; sesame oil can stimulate local circulation, moisturize and relieve itching, improve skin texture, etc., and it can also neutralize the irritation of garlic juice, so that the nasal mucosa is not irritated when taking the medicine.

[0020] Furthermore, the decoction process of the Chinese medicine in the present invention is a combination of multiple decoction processes, which are in the order of strong, gentle and strong. Among them, the first decoction process is to directly decoct for 29 to 33 minutes with strong fire, which is mainly to quickly extract some volatile and easily destroyed by high temperature effective components through short-term strong fire; the second decoction process is to soak the dregs for 50 to 60 minutes, boil them with strong fire, and then decoct them with slow fire for 80 to 90 minutes, which is mainly to thoroughly extract the effective components in the Chinese medicinal materials through long-term soaking and slow fire decoction; the third decoction process is to soak the dregs for 120 to 130 minutes, and then decoct them with strong fire for 50 to 60 minutes. The last long soaking and strong fire decoction process is because some effective components in the Chinese medicine are difficult to extract by slow fire, so long soaking and strong fire decoction are selected to extract the remaining effective components, so as to achieve the purpose of increasing the content of effective components in the medicine.

[0021] Therefore, the preparation method of a traditional Chinese medicine paste for treating rhinitis of the present invention fully improves the content of active ingredients in the medicine through special process treatment, promotes the absorption effect of the active ingredients by the nasal mucosa, reduces its irritation to the nasal cavity, and further improves the absorption effect and therapeutic effect of the medicine after being applied to the nasal cavity by adding auxiliary materials. DETAILED DESCRIPTION

[0022] In order to further explain the technical solution of the present invention, the present invention is described in detail below through specific embodiments.

[0023] Example 1 A method for preparing a Chinese medicine paste for treating rhinitis comprises the following steps: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2 parts of asarum, 3 parts of wormwood, 2 parts of mint, 9 parts of plantain, 7 parts of honeysuckle, 13 parts of angelica, 7 parts of Ligusticum chuanxiong, 15 parts of magnolia, 14 parts of Xanthium sibiricum, 7 parts of Nepeta tenuifolia, 13 parts of Forsythia suspensa, 11 parts of Scutellaria baicalensis, 5 parts of Panax notoginseng, 9 parts of Saposhnikovia divaricata, 8 parts of Notopterygium wilfordii, 3 parts of Taraxacum mongolicum, 2 parts of Vitex rotundifolia, 16 parts of Herba Eupatorii, 4 parts of Erlangjian, and 3 parts of Sophora flavescens; all the above raw materials are purchased from Anhui Hejitang Chinese Medicine Pieces Co., Ltd.; Step 2, then mixing the above-mentioned Chinese medicine powders in proportion, mixing and stirring with distilled water at a mass ratio of 1:1.2, and then adding snail liquid extract and earthworm tissue extract at a mass ratio of 1:1, and then adding cellulase and pectinase in sequence for enzymolysis, and adding papain for enzymolysis, and obtaining a Chinese medicine treatment liquid after enzymolysis; Among them, the addition amount of snail liquid extract and earthworm tissue extract was 0.12% respectively. The snail liquid extract was purchased from Xi'an Prius Bioengineering Co., Ltd., and the earthworm tissue extract was purchased from Shaanxi Fuerbang Biotechnology Co., Ltd.; The enzymatic activity of cellulase is 10000 U / g, the enzymatic activity of pectinase is 10000 U / g, the enzymatic hydrolysis conditions of cellulase and pectinase are as follows: the addition amount of cellulase is 0.25%, the addition amount of pectinase is 0.27%, the enzymatic hydrolysis temperature is 45°C, the pH is adjusted to 4.8 with 10% phosphoric acid solution or 10% sodium hydroxide solution, and the enzymatic hydrolysis time is 55 min; The enzymatic activity of papain is 10000 U / g. The enzymatic hydrolysis conditions of papain are as follows: the addition amount is 0.13%, the enzymatic hydrolysis temperature is 55°C, the pH is adjusted to 5.8 using 10% phosphoric acid solution or 10% sodium hydroxide solution, and the enzymatic hydrolysis time is 40 min. Cellulase, pectinase, and papain were purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.; The added amount of snail liquid extract, earthworm tissue extract, cellulase, pectinase and papain is calculated as a proportion of the sum of the amounts of the traditional Chinese medicine powder; Step 3, then add 5.0% glucose, 2.0% maltodextrin, 0.10% yeast extract powder and 0.50% sodium acetate to the Chinese medicinal material processing liquid, stir evenly, inoculate 0.72% Bacillus, 0.72% Lactobacillus plantarum, 0.72% Eurotium cristatum and 0.72% Lactobacillus acidophilus, and ferment at room temperature for 30 hours to obtain a Chinese medicinal material fermentation liquid; All the strains used for fermentation were purchased from Jiangsu Jiujia Biotechnology Co., Ltd. The amount of raw materials of each fermentation substrate was calculated as a percentage of the mass of the Chinese herbal medicine treatment liquid, and the inoculation ratio of each strain used for fermentation was calculated as a proportion of the mass of the Chinese herbal medicine treatment liquid; Step 4, then the obtained Chinese medicine fermentation liquid and the pig pancreas filtrate are mixed in a mass ratio of 1: 1, stirred evenly and poured into a Chinese medicine decoction machine, after three decoctions, all the obtained filtrate is mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and a Chinese medicine paste semi-finished product is obtained; The preparation method of the porcine pancreas filtrate is as follows: the porcine pancreas and distilled water are mixed in a mass ratio of 1:1.4, and the mixture is boiled and filtered through absorbent cotton gauze to obtain the filtrate, which is the porcine pancreas filtrate; The three decoction processes are as follows: The first decoction process is: decoct at high heat for 30 min, filter through absorbent cotton gauze to obtain the first filtrate and the first dregs; the second decoction process is: mix the first dregs with distilled water at a mass ratio of 1:1.5, soak for 50 min, boil at high heat, then decoct at low heat for 90 min, filter through absorbent cotton gauze to obtain the second filtrate and the second dregs; the third decoction process is: mix the second dregs with distilled water at a mass ratio of 1:1.2, soak for 130 min, decoct at high heat for 50 min, filter through absorbent cotton gauze to obtain the third filtrate and the third dregs; Step 5, then wait for the Chinese medicine paste semi-finished product to cool to 85 ° C, mix the Chinese medicine paste semi-finished product, lard paste and beeswax in a mass ratio of 1: 0.3: 0.2, and stir evenly, wait until it is completely cooled to room temperature, add 1.2% glycerol, 0.6% garlic juice, 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the Chinese medicine paste semi-finished product), stir evenly again, and finally use a 253.7 nm wavelength ultraviolet lamp to irradiate the material at a distance of 200 mm for 60 minutes, put it into a product container, and obtain a finished Chinese medicine paste.

[0024] Example 2 The difference between this embodiment and embodiment 1 is that: The Chinese medicinal materials of step 1 include the following raw materials by weight: 3 parts of asarum, 2 parts of wormwood, 3 parts of mint, 8 parts of plantain, 6 parts of honeysuckle, 12 parts of angelica, 6 parts of Ligusticum chuanxiong, 16 parts of magnolia, 13 parts of Xanthium sibiricum, 8 parts of Schizonepeta tenuifolia, 12 parts of Forsythia suspensa, 12 parts of Scutellaria baicalensis, 4 parts of Panax notoginseng, 8 parts of Saposhnikovia divaricata, 7 parts of Notopterygium wilfordii, 4 parts of Taraxacum mongolicum, 3 parts of Vitex rotundifolia, 15 parts of Herba Eupatorii, 3 parts of Erlangjian, and 4 parts of Sophora flavescens.

[0025] Example 3 The difference between this embodiment and embodiment 1 is that the usage ratio of the fermentation substrate in step 3 is different, specifically: the fermentation substrate includes 4.5% glucose, 1.8% maltodextrin, 0.12% yeast extract powder and 0.46% sodium acetate.

[0026] Example 4 The difference between this embodiment and embodiment 1 is that the usage ratio of the auxiliary materials in step 5 is different, specifically, the auxiliary materials include 1.5% glycerol, 1.3% garlic juice, 0.5% sesame oil and 0.2% chitosan.

[0027] Comparative Example 1 The difference from Example 1 is that the amount of raw materials of the Chinese medicine powder in Comparative Example 1 is adjusted, specifically: The Chinese medicinal materials of step 1 include the following raw materials by weight: 4 parts of asarum, 4 parts of wormwood, 4 parts of mint, 5 parts of plantain, 5 parts of honeysuckle, 7 parts of angelica, 5 parts of Ligusticum chuanxiong, 10 parts of magnolia, 10 parts of Xanthium sibiricum, 5 parts of Schizonepeta tenuifolia, 9 parts of Forsythia suspensa, 8 parts of Scutellaria baicalensis, 6 parts of Panax notoginseng, 6 parts of Saposhnikovia divaricata, 5 parts of Notopterygium wilfordii, 4 parts of Taraxacum mongolicum, 4 parts of Vitex rotundifolia, 12 parts of Herba Eupatorii, 5 parts of Erlangjian, and 5 parts of Sophora flavescens.

[0028] Comparative Example 2 The difference from Example 1 is that the enzymatic hydrolysis process of step 2 and the fermentation process of step 3 are omitted in Comparative Example 2, specifically: A method for preparing a Chinese medicine paste for treating rhinitis comprises the following steps: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2 parts of asarum, 3 parts of wormwood, 2 parts of mint, 9 parts of plantain, 7 parts of honeysuckle, 13 parts of angelica, 7 parts of Ligusticum chuanxiong, 15 parts of magnolia, 14 parts of Xanthium sibiricum, 7 parts of Nepeta tenuifolia, 13 parts of Forsythia suspensa, 11 parts of Scutellaria baicalensis, 5 parts of Panax notoginseng, 9 parts of Saposhnikovia divaricata, 8 parts of Notopterygium wilfordii, 3 parts of Taraxacum mongolicum, 2 parts of Vitex rotundifolia, 16 parts of Herba Eupatorii, 4 parts of Erlangjian, and 3 parts of Sophora flavescens; all the above raw materials are purchased from Anhui Hejitang Chinese Medicine Pieces Co., Ltd.; Step 2, then mixing the above-mentioned Chinese medicine powders according to proportions, and mixing with distilled water at a mass ratio of 1:1.2 to obtain a Chinese medicine processing liquid; Step 3, then the obtained Chinese medicinal material processing liquid and the pig pancreas filtrate are mixed in a mass ratio of 1: 1, stirred evenly and poured into a Chinese medicine decoction machine, after three decoctions, all the obtained filtrate is mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and the Chinese medicine paste semi-finished product is obtained; The preparation method of the porcine pancreas filtrate is as follows: the porcine pancreas and distilled water are mixed in a mass ratio of 1:1.4, and the mixture is boiled and filtered through absorbent cotton gauze to obtain the filtrate, which is the porcine pancreas filtrate; The three decoction processes are as follows: The first decoction process is: decoct at high heat for 30 min, filter through absorbent cotton gauze to obtain the first filtrate and the first dregs; the second decoction process is: mix the first dregs with distilled water at a mass ratio of 1:1.5, soak for 50 min, boil at high heat, then decoct at low heat for 90 min, filter through absorbent cotton gauze to obtain the second filtrate and the second dregs; the third decoction process is: mix the second dregs with distilled water at a mass ratio of 1:1.2, soak for 130 min, decoct at high heat for 50 min, filter through absorbent cotton gauze to obtain the third filtrate and the third dregs; Step 4, then wait for the Chinese medicine paste semi-finished product to cool to 85 ° C, mix the Chinese medicine paste semi-finished product, lard paste and beeswax in a mass ratio of 1: 0.3: 0.2, and stir evenly, wait until it is completely cooled to room temperature, add 1.2% glycerol, 0.6% garlic juice, 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the Chinese medicine paste semi-finished product), stir evenly again, and finally use a 253.7 nm wavelength ultraviolet lamp to irradiate the material at a distance of 200 mm for 60 minutes, put it into a product container, and obtain a finished Chinese medicine paste.

[0029] Comparative Example 3 The difference from Example 1 is that the enzymatic hydrolysis process of step 2 is omitted in Comparative Example 3, specifically: A method for preparing a Chinese medicine paste for treating rhinitis comprises the following steps: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2 parts of asarum, 3 parts of wormwood, 2 parts of mint, 9 parts of plantain, 7 parts of honeysuckle, 13 parts of angelica, 7 parts of Ligusticum chuanxiong, 15 parts of magnolia, 14 parts of Xanthium sibiricum, 7 parts of Nepeta tenuifolia, 13 parts of Forsythia suspensa, 11 parts of Scutellaria baicalensis, 5 parts of Panax notoginseng, 9 parts of Saposhnikovia divaricata, 8 parts of Notopterygium wilfordii, 3 parts of Taraxacum mongolicum, 2 parts of Vitex rotundifolia, 16 parts of Herba Eupatorii, 4 parts of Erlangjian, and 3 parts of Sophora flavescens; all the above raw materials are purchased from Anhui Hejitang Chinese Medicine Pieces Co., Ltd.; Step 2, then mixing the above-mentioned Chinese medicine powders according to proportions, and mixing with distilled water at a mass ratio of 1:1.2 to obtain a Chinese medicine processing liquid; Step 3, then add 5.0% glucose, 2.0% maltodextrin, 0.10% yeast extract powder and 0.50% sodium acetate to the Chinese medicinal material processing liquid, stir evenly, inoculate 0.72% Bacillus, 0.72% Lactobacillus plantarum, 0.72% Eurotium cristatum and 0.72% Lactobacillus acidophilus, and ferment at room temperature for 30 hours to obtain a Chinese medicinal material fermentation liquid; All the strains used for fermentation were purchased from Jiangsu Jiujia Biotechnology Co., Ltd.; the amount of raw materials of each fermentation substrate was calculated as a percentage of the mass of the Chinese herbal medicine processing liquid, and the inoculation ratio of each strain used for fermentation was calculated as a proportion of the mass of the Chinese herbal medicine processing liquid; Step 4, then the obtained Chinese medicine fermentation liquid and the pig pancreas filtrate are mixed in a mass ratio of 1: 1, stirred evenly and poured into a Chinese medicine decoction machine, after three decoctions, all the obtained filtrate is mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and a Chinese medicine paste semi-finished product is obtained; The preparation method of the porcine pancreas filtrate is as follows: the porcine pancreas and distilled water are mixed in a mass ratio of 1:1.4, and the mixture is boiled and filtered through absorbent cotton gauze to obtain the filtrate, which is the porcine pancreas filtrate; The three decoction processes are as follows: The first decoction process is: decoct at high heat for 30 min, filter through absorbent cotton gauze to obtain the first filtrate and the first dregs; the second decoction process is: mix the first dregs with distilled water at a mass ratio of 1:1.5, soak for 50 min, boil at high heat, then decoct at low heat for 90 min, filter through absorbent cotton gauze to obtain the second filtrate and the second dregs; the third decoction process is: mix the second dregs with distilled water at a mass ratio of 1:1.2, soak for 130 min, decoct at high heat for 50 min, filter through absorbent cotton gauze to obtain the third filtrate and the third dregs; Step 5, then wait for the Chinese medicine paste semi-finished product to cool to 85 ° C, mix the Chinese medicine paste semi-finished product, lard paste and beeswax in a mass ratio of 1: 0.3: 0.2, and stir evenly, wait until it is completely cooled to room temperature, add 1.2% glycerol, 0.6% garlic juice, 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the Chinese medicine paste semi-finished product), stir evenly again, and finally use a 253.7 nm wavelength ultraviolet lamp to irradiate the material at a distance of 200 mm for 60 minutes, put it into a product container, and obtain a finished Chinese medicine paste.

[0030] Comparative Example 4 The difference from Example 1 is that the fermentation process of step 3 is omitted in Comparative Example 4, which is specifically as follows: A method for preparing a Chinese medicine paste for treating rhinitis comprises the following steps: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2 parts of asarum, 3 parts of wormwood, 2 parts of mint, 9 parts of plantain, 7 parts of honeysuckle, 13 parts of angelica, 7 parts of Ligusticum chuanxiong, 15 parts of magnolia, 14 parts of Xanthium sibiricum, 7 parts of Nepeta tenuifolia, 13 parts of Forsythia suspensa, 11 parts of Scutellaria baicalensis, 5 parts of Panax notoginseng, 9 parts of Saposhnikovia divaricata, 8 parts of Notopterygium wilfordii, 3 parts of Taraxacum mongolicum, 2 parts of Vitex rotundifolia, 16 parts of Herba Eupatorii, 4 parts of Erlangjian, and 3 parts of Sophora flavescens; all the above raw materials are purchased from Anhui Hejitang Chinese Medicine Pieces Co., Ltd.; Step 2, then mixing the above-mentioned Chinese medicine powders in proportion, mixing and stirring with distilled water at a mass ratio of 1:1.2, and then adding snail liquid extract and earthworm tissue extract at a mass ratio of 1:1, and then adding cellulase and pectinase in sequence for enzymolysis, and adding papain for enzymolysis, and obtaining a Chinese medicine treatment liquid after enzymolysis; Among them, the addition amount of snail liquid extract and earthworm tissue extract was 0.12% respectively. The snail liquid extract was purchased from Xi'an Prius Bioengineering Co., Ltd., and the earthworm tissue extract was purchased from Shaanxi Fuerbang Biotechnology Co., Ltd.; The enzymatic activity of cellulase is 10000 U / g, the enzymatic activity of pectinase is 10000 U / g, the enzymatic hydrolysis conditions of cellulase and pectinase are as follows: the addition amount of cellulase is 0.25%, the addition amount of pectinase is 0.27%, the enzymatic hydrolysis temperature is 45°C, the pH is adjusted to 4.8 with 10% phosphoric acid solution or 10% sodium hydroxide solution, and the enzymatic hydrolysis time is 55 min; The activity of papain was 10000 U / g. The enzymatic hydrolysis conditions of papain were as follows: the amount added was 0.13% of the sum of the amounts of the Chinese medicine powders, the enzymatic hydrolysis temperature was 55°C, the pH was adjusted to 5.8 using 10% phosphoric acid solution or 10% sodium hydroxide solution, and the enzymatic hydrolysis time was 40 min. Cellulase, pectinase and papain were purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.; the amount of snail fluid extract, earthworm tissue extract, cellulase, pectinase and papain added was calculated based on the proportion of the sum of the amounts of Chinese medicine powder; Step 3, then the obtained Chinese medicine treatment liquid and the porcine pancreas filtrate are mixed in a mass ratio of 1: 1, stirred evenly and poured into a Chinese medicine decoction machine, after three decoctions, all the obtained filtrate is mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and the Chinese medicine paste semi-finished product is obtained; The preparation method of the porcine pancreas filtrate is as follows: the porcine pancreas and distilled water are mixed in a mass ratio of 1:1.4, and the mixture is boiled and filtered through absorbent cotton gauze to obtain the filtrate, which is the porcine pancreas filtrate; The three decoction processes are as follows: The first decoction process is: decoct at high heat for 30 min, filter through absorbent cotton gauze to obtain the first filtrate and the first dregs; the second decoction process is: mix the first dregs with distilled water at a mass ratio of 1:1.5, soak for 50 min, boil at high heat, then decoct at low heat for 90 min, filter through absorbent cotton gauze to obtain the second filtrate and the second dregs; the third decoction process is: mix the second dregs with distilled water at a mass ratio of 1:1.2, soak for 130 min, decoct at high heat for 50 min, filter through absorbent cotton gauze to obtain the third filtrate and the third dregs; Step 4, then wait for the Chinese medicine paste semi-finished product to cool to 85 ° C, mix the Chinese medicine paste semi-finished product, lard paste and beeswax in a mass ratio of 1: 0.3: 0.2, and stir evenly, wait until it is completely cooled to room temperature, add 1.2% glycerol, 0.6% garlic juice, 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the Chinese medicine paste semi-finished product), stir evenly again, and finally use a 253.7 nm wavelength ultraviolet lamp to irradiate the material at a distance of 200 mm for 60 minutes, put it into a product container, and obtain a finished Chinese medicine paste.

[0031] Comparative Example 5 The difference from Example 1 is that in step 3 of Comparative Example 5, no fermentation substrate is added, specifically: A method for preparing a Chinese medicine paste for treating rhinitis comprises the following steps: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2 parts of asarum, 3 parts of wormwood, 2 parts of mint, 9 parts of plantain, 7 parts of honeysuckle, 13 parts of angelica, 7 parts of Ligusticum chuanxiong, 15 parts of magnolia, 14 parts of Xanthium sibiricum, 7 parts of Nepeta tenuifolia, 13 parts of Forsythia suspensa, 11 parts of Scutellaria baicalensis, 5 parts of Panax notoginseng, 9 parts of Saposhnikovia divaricata, 8 parts of Notopterygium wilfordii, 3 parts of Taraxacum mongolicum, 2 parts of Vitex rotundifolia, 16 parts of Herba Eupatorii, 4 parts of Erlangjian, and 3 parts of Sophora flavescens; all the above raw materials are purchased from Anhui Hejitang Chinese Medicine Pieces Co., Ltd.; Step 2, then mixing the above-mentioned Chinese medicine powders in proportion, mixing and stirring with distilled water at a mass ratio of 1:1.2, and then adding snail liquid extract and earthworm tissue extract at a mass ratio of 1:1, and then adding cellulase and pectinase in sequence for enzymolysis, and adding papain for enzymolysis, and obtaining a Chinese medicine treatment liquid after enzymolysis; Among them, the addition amount of snail liquid extract and earthworm tissue extract was 0.12% respectively. The snail liquid extract was purchased from Xi'an Prius Bioengineering Co., Ltd., and the earthworm tissue extract was purchased from Shaanxi Fuerbang Biotechnology Co., Ltd.; The enzymatic activity of cellulase is 10000 U / g, the enzymatic activity of pectinase is 10000 U / g, the enzymatic hydrolysis conditions of cellulase and pectinase are: the addition amount of cellulase is 0.25%, the addition amount of pectinase is 0.27%, the enzymatic hydrolysis temperature is 45°C, the pH is adjusted to 4.8 with 10% phosphoric acid solution or 10% sodium hydroxide solution, and the enzymatic hydrolysis time is 55 min; The activity of papain was 10000 U / g. The enzymatic hydrolysis conditions of papain were as follows: the amount added was 0.13% of the sum of the amounts of the Chinese medicine powders, the enzymatic hydrolysis temperature was 55°C, the pH was adjusted to 5.8 using 10% phosphoric acid solution or 10% sodium hydroxide solution, and the enzymatic hydrolysis time was 40 min. Cellulase, pectinase and papain were purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd.; the amount of snail fluid extract, earthworm tissue extract, cellulase, pectinase and papain added was calculated based on the proportion of the sum of the amounts of Chinese medicine powder; Step 3, then inoculating 0.72% Bacillus, 0.72% Lactobacillus plantarum, 0.72% Eurotium cristatum and 0.72% Lactobacillus acidophilus into the Chinese medicinal material processing liquid, and fermenting it at room temperature for 30 hours to obtain the Chinese medicinal material fermentation liquid; All the fermentation strains were purchased from Jiangsu Jiujia Biotechnology Co., Ltd.; the inoculation ratio of each fermentation strain was calculated based on the mass usage of the Chinese medicinal material processing liquid; Step 4, then the obtained Chinese medicine fermentation liquid and the pig pancreas filtrate are mixed in a mass ratio of 1: 1, stirred evenly and poured into a Chinese medicine decoction machine, after three decoctions, all the obtained filtrate is mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and a Chinese medicine paste semi-finished product is obtained; The preparation method of the porcine pancreas filtrate is as follows: the porcine pancreas and distilled water are mixed in a mass ratio of 1:1.4, and the mixture is boiled and filtered through absorbent cotton gauze to obtain the filtrate, which is the porcine pancreas filtrate; The three decoction processes are as follows: The first decoction process is: decoct at high heat for 30 min, filter through absorbent cotton gauze to obtain the first filtrate and the first dregs; the second decoction process is: mix the first dregs with distilled water at a mass ratio of 1:1.5, soak for 50 min, boil at high heat, then decoct at low heat for 90 min, filter through absorbent cotton gauze to obtain the second filtrate and the second dregs; the third decoction process is: mix the second dregs with distilled water at a mass ratio of 1:1.2, soak for 130 min, decoct at high heat for 50 min, filter through absorbent cotton gauze to obtain the third filtrate and the third dregs; Step 5, then wait for the Chinese medicine paste semi-finished product to cool to 85 ° C, mix the Chinese medicine paste semi-finished product, lard paste and beeswax in a mass ratio of 1:0.3:0.2, and stir evenly, wait until it is completely cooled to room temperature, add 1.2% glycerol, 0.6% garlic juice, 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the Chinese medicine paste semi-finished product), stir evenly again, and finally use a 253.7 nm wavelength ultraviolet lamp to irradiate the material at a distance of 200 mm for 60 min, put it into a product container, and obtain a finished Chinese medicine paste.

[0032] Comparative Example 6 The difference from Example 1 is that the first decoction process is omitted in the decoction process of Comparative Example 6, and the decoction process is specifically as follows: The first decoction process is: boil over high heat and then simmer over low heat for 90 min, and filter through absorbent cotton gauze to obtain the first filtrate and the first residue; the second decoction process is: mix the first residue and distilled water in a mass ratio of 1:1.2, soak for 130 min, and then simmer over high heat for 50 min, and filter through absorbent cotton gauze to obtain the second filtrate and the second residue.

[0033] Comparative Example 7 The difference from Example 1 is that the second decoction process is omitted in the decoction process of Comparative Example 7, and the decoction process is specifically as follows: The first decoction process is: decoct at high heat for 30 min, and filter through absorbent cotton gauze to obtain the first filtrate and the first residue; the second decoction process is: mix the first residue and distilled water in a mass ratio of 1:1.2, soak for 130 min, decoct at high heat for 50 min, and filter through absorbent cotton gauze to obtain the second filtrate and the second residue.

[0034] Comparative Example 8 The difference from Example 1 is that the third decoction process is omitted in the decoction process of Comparative Example 8, and the decoction process is specifically as follows: The first decoction process is: decoct at high heat for 30 min, and filter through absorbent cotton gauze to obtain the first filtrate and the first residue; the second decoction process is: mix the first residue with distilled water in a mass ratio of 1:1.5, soak for 50 min, boil over high heat and then decoct over low heat for 90 min, and filter through absorbent cotton gauze to obtain the second filtrate and the second residue.

[0035] Comparative Example 9 The difference from Example 1 is that in step 2 of Comparative Example 8, no snail fluid extract and earthworm tissue extract are added.

[0036] Comparative Example 10 The difference from Example 1 is that in step 5 of Comparative Example 9, no garlic juice is added as the auxiliary material, specifically: the auxiliary material includes 1.2% glycerol, 0.3% sesame oil and 0.1% chitosan (the amount of the raw material of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0037] Comparative Example 11 The difference from Example 1 is that in step 5 of Comparative Example 10, no glycerol is added to the auxiliary materials. Specifically, the auxiliary materials include 0.6% garlic juice, 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0038] Comparative Example 12 The difference from Example 1 is that in step 5 of Comparative Example 11, no glycerol and garlic juice are added as auxiliary materials. Specifically, the auxiliary materials include 0.3% sesame oil and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0039] Comparative Example 13 The difference from Example 1 is that in step 5 of Comparative Example 12, no sesame oil is added to the auxiliary materials. Specifically, the auxiliary materials include 1.2% glycerol, 0.6% garlic juice and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0040] Comparative Example 14 The difference from Example 1 is that in step 5 of Comparative Example 13, no chitosan is added as the auxiliary material, specifically, the auxiliary material includes 1.2% glycerol, 0.6% garlic juice and 0.3% sesame oil (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0041] Comparative Example 15 The difference from Example 1 is that in step 5 of Comparative Example 14, sesame oil and chitosan are not added as auxiliary materials. Specifically, the auxiliary materials include 1.2% glycerol and 0.6% garlic juice (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0042] Comparative Example 16 The difference from Example 1 is that in step 5 of Comparative Example 15, no sesame oil and glycerin are added as auxiliary materials. Specifically, the auxiliary materials include 0.6% garlic juice and 0.1% chitosan (the amount of the raw materials of each auxiliary material is calculated as a percentage of the mass of the semi-finished Chinese medicine paste).

[0043] Experimental Example 1 (1) Experimental group The Chinese medicinal pastes prepared in Examples 1 to 4 and Comparative Examples 1 to 9 and Comparative Example 14 were taken as the experimental group; (2) Competitive product experimental group The aqueous solution containing 0.5% chlorhexidine acetate was used as the competitive product experimental group; (3) Antibacterial test The antibacterial rate test is based on the carrier immersion quantitative antibacterial test in Article 5.1.2 of WS / T 650-2019 "Antibacterial and Antibacterial Effect Evaluation Methods". The indicator bacteria is Staphylococcus aureus (FSCC223004 / ATCC 6538), the action time is 2 minutes, and the antibacterial rate is calculated as follows: Inhibition rate (%) = [bacterial quantity in the positive control group (CFU / tablet) - bacterial quantity in the sample group (CFU / tablet)] / bacterial quantity in the positive control group (CFU / tablet) * 100%; The antibacterial test results are shown in Table 1.

[0044] Table 1 Antibacterial rate of each group of Chinese medicine paste

[0045] It can be seen from Table 1 that the formula variation of the Chinese medicinal raw materials in Examples 1 to 2 varies within the formula ratio range specified in the present invention, which has little effect on the antibacterial rate. If the formula of the Chinese medicinal raw materials exceeds the formula ratio range specified in the present invention, it will affect the antibacterial effect (such as Comparative Example 1). In Examples 3 to 4, the fermentation substrate and the auxiliary materials vary within the specified range, which has little effect on the antibacterial effect. From the comparison of the antibacterial effects of Examples 1 to 4 and the competitive experimental group, it can be seen that the Chinese medicine paste prepared by the present invention has a better antibacterial effect than the conventional 0.5% chlorhexidine acetate aqueous solution; from Comparative Examples 2 to 4, it can be seen that the absence of enzymatic hydrolysis or fermentation steps will lead to a significant decrease in the antibacterial rate, especially the absence of fermentation is the most obvious, because fermentation is the most important step of the present invention, and the enzymatic hydrolysis step can greatly promote the fermentation effect, and when both enzymatic hydrolysis and fermentation are not performed, the continued decrease is not large compared to only removing the enzymatic hydrolysis or only removing the fermentation step; from Comparative Example 4, it can be seen that not adding a fermentation substrate before fermentation will lead to a significant decrease in the antibacterial rate, and although there is a certain improvement compared to not fermenting, the improvement is lower than the improvement after adding the fermentation substrate, which is because the addition of the fermentation substrate can To significantly promote the fermentation effect; from comparative examples 6 to 8, it can be seen that removing any one of the three decoction processes will lead to a decrease in the antibacterial effect. This is because the three decoction processes all have corresponding extractions of different types of effective ingredients. The loss of some effective ingredients after removal will affect the antibacterial effect. For example, removing the first process will lead to the loss of some volatile and easily destroyed by high temperature components, removing the second process will lead to the incomplete extraction of the main effective ingredients or destruction by high temperature, and removing the third process will lead to the failure of some processes that require long-term high heat to extract; from comparative example 9, it can be seen that removing the snail liquid extract and earthworm tissue extract from the auxiliary materials will lead to a significant decrease in the antibacterial effect, which also reflects that the snail liquid extract and earthworm tissue extract have antibacterial ability. From comparative example 14, it can be seen that removing chitosan from the auxiliary materials will also lead to a decrease in the antibacterial effect, which also proves that chitosan has a broad-spectrum antibacterial effect, can inhibit the growth of bacteria and fungi, and has an anti-inflammatory effect.

[0046] Experimental Example 2 (1) Experimental group and control group The Chinese medicine pastes prepared in Examples 1 to 4 were taken as the experimental group, and the Chinese medicine pastes prepared in Comparative Examples 11, 13, and 16 were taken as the comparative group; (2) Penetration effect test The penetration effect was tested by the Franz diffusion cell transdermal test. The pigskin was fixed between the diffusion chamber and the receiving chamber of the transdermal diffusion instrument, with the inner side of the pigskin facing the receiving chamber and the skin stratum corneum facing the diffusion chamber, so that it was in close contact. The pigskin diffusion chamber was covered with Chinese medicine pastes of different experimental groups and control groups, and physiological saline receiving liquid was injected into the receiving chamber. The liquid surface just contacted the inner layer of the skin, and the electromagnetic stirrer was started to stir at a constant speed and keep the temperature constant. After 3 h, 6 h, and 9 h of penetration, the transdermal absorption liquid was collected, and its absorbance was measured by ultraviolet spectrophotometry and recorded. Among them, the preparation of in vitro pigskin: take the pigskin, remove the pig hair, scrape off the subcutaneous fat tissue, make the thickness of all the pigskin uniform, and place it in the refrigerator for storage. Among them, the absorbance was detected by ultraviolet spectrophotometer, and the detection wavelength was 300 nm.

[0047] Table 2 Penetration effect of each group of Chinese medicine paste

[0048] It can be seen from the penetration effect in Table 2 that the penetration effects of experimental groups 1 to 4 are not much different, and they are all better than the control group. It can be seen from the penetration effects of control group 11 (no glycerin added), control group 13 (no sesame oil added), and control group 16 (no glycerin and sesame oil added) that the absence of glycerin or sesame oil in the excipients will lead to a significant decrease in the penetration effect, especially when glycerin is not added. In control group 16, when neither glycerin nor sesame oil is added, the penetration effect will further decrease, and the further decrease is greater than the decrease when no single component is added. This is because glycerin and sesame oil have good penetrating and infectious effects, which can help the active ingredients in the plaster quickly penetrate into the deep layers of the skin, improve the absorption effect of the drug, and accelerate the effect of the drug on the lesions.

[0049] Experimental Example 3 (1) Experimental group and control group The Chinese medicine paste prepared in Example 1 was taken as the experimental group, and the Chinese medicine pastes prepared in Comparative Example 1, Comparative Examples 3-4, and Comparative Examples 9-16 were taken as the comparative group; (2) Competitive product control group A 0.5% chlorhexidine acetate aqueous solution was mixed with lard ointment in a ratio of 1:1 as the competitive product experimental group.

[0050] (3) Sensory test Sensory tests were conducted on the experimental group, comparison group and competitor control group. The sensory test items included nasal comfort after use, improvement of rhinitis and recurrence after drug discontinuation.

[0051] The method of sensory test is as follows: 100 testers with rhinitis are selected, each tester evaluates one group, and 10 people are tested in each group. The evaluation method is to apply ointment to the affected part of the nasal cavity, once in the morning and once in the evening, and stop using it for 30 consecutive days and then score it. After stopping use for 30 days, observe the recurrence and score it. The scoring standard is shown in Table 3. Finally, the average value is taken. The test results are shown in Table 4.

[0052] Table 3 Scoring criteria

[0053] Table 4 Sensory test results

[0054] From the sensory test results of the experimental group, the comparison group, and the competitive experimental group in Table 4, it can be seen that the three indicators of the experimental group 1 are significantly better than those of the comparative group 1. This is because the ratio of the comparative example 1 has changed, and the effect of the overall prescription has decreased, indicating that the prescription ratio range of the present invention is optimal. The three indicators of the Chinese medicine paste of Example 1 and Comparative Example 1 are better than those of the competitive experimental group, namely, skin comfort, improvement of rhinitis, and recurrence after discontinuation of use. In particular, the three indicators of Comparative Examples 3 to 4 are significantly lower than those of Example 1. This is because enzymatic hydrolysis or fermentation is extremely important for the extraction of effective ingredients in Chinese medicinal materials. It can be seen from the comparative group 9 that the removal of the snail liquid extract and the earthworm tissue extract in the auxiliary materials will lead to a significant decrease in the antibacterial effect, and the three indicators will also deteriorate significantly. It can be seen from the experimental group 1 and the comparison groups 11, 13, and 16 that the addition of glycerin and sesame oil can significantly improve skin comfort, because glycerin and sesame oil increase the penetration effect, make the paste better absorbed, and reduce the drug residues that are not easily absorbed on the skin surface; it can be seen from the experimental group 1 and the comparison groups 10 and 14 that without the addition of garlic juice or chitosan, the improvement of body tinea decreased, because garlic juice and chitosan have certain anti-inflammatory and antibacterial effects; the comparison groups 3~4 and the competitive product experimental group show that although the improvement of body tinea is acceptable, it is more likely to relapse, which shows that although the simple antibacterial ingredients can improve body tinea, it is prone to relapse.

[0055] In addition, the present invention has currently treated 95 patients in clinical practice, of which 63 were basically cured, 17 had significant effects, 12 were improved, and 3 were ineffective.

[0056] Usage and treatment course: The patient should clean his hands first, then clean the nasal cavity, use a cotton swab to take an appropriate amount of the Chinese medicine paste and apply it to both sides of the inner wall of the nasal cavity, and use both hands to gently massage both sides of the nose. After the massage is absorbed, apply it once a day in the morning and evening. The symptoms can be significantly relieved in 7 to 15 days, and the patient can be basically cured in 25 to 40 days without recurrence.

[0057] Case 1: Zhou, female, 28 years old. Medical history: frequent sneezing, decreased sense of smell, continuous sneezing, head discomfort, pain or numbness in the maxillofacial area, diagnosed as sinusitis. The Chinese medicine paste of the present invention was administered to the nasal cavity twice a day. After 23 days, the frequency of sneezing was greatly reduced, and the sense of smell was basically restored. After continuing to use it for 35 days, all symptoms disappeared, and nasal breathing and sense of smell returned to normal. After a half-year follow-up, there was no recurrence.

[0058] Case 2, Li, male, 40. Medical history: When the cold and hot air environment alternates, the patient sneezes continuously, the nasal cavity is blocked, the nasal congestion is serious, the local mucosal congestion and edema in the nasal cavity cause the internal swelling of the nasal cavity. The nasal secretions increase, the nasal mucus is clear, and the symptoms such as nasal itching. The patient was diagnosed with allergic rhinitis and the Chinese medicine paste of the present invention was administered to the affected part of the nasal cavity 3 times a day. After 10 days, the congestion and edema of the nasal mucosa subsided, and the nasal discomfort symptoms disappeared in about 30 days. There was no recurrence after 5 months of follow-up.

[0059] The above embodiments do not limit the product form and style of the present invention. Any appropriate changes or modifications made by ordinary technicians in the relevant technical field should be deemed to be within the patent scope of the present invention.

Claims

1. A method for preparing a Chinese medicine paste for treating rhinitis, characterized in that: The following steps are involved: Step 1: First, take the air-dried Chinese medicinal material raw materials, crush and grind them respectively, and then sieve them to make 200-mesh Chinese medicinal powder; The Chinese medicinal materials include the following raw materials by weight: 2-3 parts of asarum, 2-3 parts of wormwood, 2-3 parts of mint, 8-9 parts of plantain, 6-7 parts of honeysuckle, 12-13 parts of angelica, 6-7 parts of ligusticum, 15-16 parts of magnolia, 13-14 parts of xanthium, 7-8 parts of schizonepeta, 12-13 parts of forsythia, 11-12 parts of scutellaria, 4-5 parts of panax notoginseng, 8-9 parts of siler, 7-8 parts of notopterygium, 3-4 parts of dandelion, 2-3 parts of vitex rotundus, 15-16 parts of scutellaria, 3-4 parts of erlang sword, and 3-4 parts of sophora flavescens; Step 2, then mixing the above-mentioned Chinese medicine powders in proportion, mixing and stirring with distilled water at a mass ratio of 1:1.2-1.3, and then adding snail liquid extract and earthworm tissue extract, and then adding cellulase and pectinase in sequence for enzymolysis, and adding papain for enzymolysis, and obtaining a Chinese medicine treatment liquid after enzymolysis; Step 3, then adding the fermentation substrate to the Chinese medicinal material processing liquid, stirring evenly, inoculating Bacillus, Lactobacillus plantarum, Eurotium cristatum and Lactobacillus acidophilus, standing and fermenting at room temperature, and obtaining the Chinese medicinal material fermentation liquid after fermenting for 26 to 32 hours; The fermentation substrate includes the following raw materials: 4.5% to 5.0% glucose, 1.8% to 2.1% maltodextrin, 0.09% to 0.13% yeast extract powder, and 0.45% to 0.52% sodium acetate. The amount of the raw materials of the fermentation substrate is calculated as a percentage of the mass of the traditional Chinese medicine processing liquid; Step 4, then the obtained Chinese medicine fermentation liquid and the pig pancreas filtrate are mixed in a mass ratio of 1:0.9-1.1, stirred evenly and poured into a Chinese medicine decoction machine, after multiple decoctions, all the obtained filtrates are mixed, stirred evenly and poured into the Chinese medicine decoction machine for collecting juice, and the juice is collected into a viscous state, and a Chinese medicine paste semi-finished product is obtained; Step 5, then wait for the Chinese medicine paste semi-finished product to cool to 80-85° C., mix the Chinese medicine paste semi-finished product, lard cream and beeswax in a mass ratio of 1:0.3-0.5:0.2-0.6, and stir evenly, wait until it is completely cooled to room temperature, add auxiliary materials and stir evenly again, and finally put it into a product container after ultraviolet disinfection to obtain a finished Chinese medicine paste; The auxiliary materials include the following raw materials: 1.0%-1.5% glycerol, 0.5%-1.5% garlic juice, 0.2%-0.5% sesame oil and 0.05%-0.2% chitosan, and the amount of the auxiliary materials is calculated as a percentage of the mass of the semi-finished Chinese medicine paste.

2. The method for preparing a Chinese medicine paste for treating rhinitis according to claim 1, characterized in that: In step 2, the mass ratio of the snail liquid extract and the earthworm tissue extract is 1:1-2, and the added amounts of the snail liquid extract and the earthworm tissue extract account for 0.12%-0.36% of the sum of the amounts of each Chinese medicine powder.

3. The method for preparing a Chinese medicine paste for treating rhinitis according to claim 1, characterized in that: In step 2, the enzymatic activity of cellulase is 10000 U / g, the enzymatic activity of pectinase is 10000 U / g, and the enzymatic hydrolysis conditions of cellulase and pectinase are as follows: the amount of cellulase added accounts for 0.21%~0.36% of the sum of the amounts of each Chinese medicine powder, the amount of pectinase added accounts for 0.19%~0.31% of the sum of the amounts of each Chinese medicine powder, the enzymatic hydrolysis temperature is 40~45°C, the pH is adjusted to 4.8~5.3, and the enzymatic hydrolysis time is 55~60min.

4. The method for preparing a Chinese medicinal paste for treating rhinitis according to claim 1, characterized in that: In step 2, the enzymatic activity of papain is: 10000 U / g, and the enzymatic hydrolysis conditions of papain are: the added amount accounts for 0.12%~0.13% of the sum of the amounts of each Chinese medicine powder, the enzymatic hydrolysis temperature is 42~60°C, the pH is adjusted to 4.5~6.0, and the enzymatic hydrolysis time is 40~55 min.

5. The method for preparing a Chinese medicinal paste for treating rhinitis according to claim 1, characterized in that: In step 3, the inoculation ratios of Bacillus, Lactobacillus plantarum, Eurotium cristatum and Lactobacillus acidophilus are respectively 0.70% to 0.75% of the mass amount of the traditional Chinese medicine processing liquid.

6. The method for preparing a Chinese medicinal paste for treating rhinitis according to claim 1, characterized in that: In step 4, the preparation method of the porcine pancreas filtrate is: mixing the porcine pancreas and distilled water in a mass ratio of 1:1.4-1.5, boiling and filtering through absorbent cotton gauze, and the obtained filtrate is the porcine pancreas filtrate.

7. The method for preparing a Chinese medicinal paste for treating rhinitis according to claim 1, characterized in that: In step 4, the decoction process is a three-step decoction process, wherein: The first decoction process is: decoction at high heat for 29-33 min, and filter through absorbent cotton gauze to obtain the first filtrate and the first residue; the second decoction process is: mix the first residue with distilled water in a mass ratio of 1:1.5-1.6, soak for 50-60 min, boil over high heat, and then decoct over low heat for 80-90 min, and filter through absorbent cotton gauze to obtain the second filtrate and the second residue; the third decoction process is: mix the second residue with distilled water in a mass ratio of 1:1-1.2, soak for 120-130 min, decoct over high heat for 50-60 min, and filter through absorbent cotton gauze to obtain the third filtrate and the third residue.

8. The method for preparing a Chinese medicinal paste for treating rhinitis according to claim 1, characterized in that: In step 5, the process of ultraviolet disinfection is: using an ultraviolet lamp with a wavelength of 253.7 nm to irradiate the material at a distance of 200-300 mm for 50-60 min.

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