Isolated heart perfusion experiment device and isolated heart perfusion experiment method

Abstract

The invention is applicable to the technical field of heart perfusion devices, and provides an isolated heart perfusion experimental device and an isolated heart perfusion experimental method, blood in an isolated heart can be squeezed out by arranging a fixing mechanism, blood coagulation in the heart is avoided, meanwhile, the heart can be fixed, and the device is convenient to use. Meanwhile, the transverse position, the longitudinal position and the deflection angle of the fixed heart can be adjusted in cooperation with the adjusting mechanism, so that the heart aorta can be aligned with a perfusion conveying pipe, subsequent perfusion of the heart is facilitated, the heart aorta can be expanded by arranging the injection mechanism, and the injection efficiency is improved. Therefore, the conveying pipe can be conveniently inserted into the aorta, the efficiency of inserting the conveying pipe into the heart aorta can be improved, the inner wall of the heart aorta can be adsorbed and fixed to the outer portion of the conveying pipe in cooperation with the limiting structure at the end of the conveying pipe, and therefore the heart aorta can be ligated without additionally arranging a binding wire; and the perfusion efficiency of the device is further improved.

Description

Technical Field

[0001] The invention belongs to the technical field of cardiac perfusion devices, and in particular relates to an isolated cardiac perfusion experimental device and an isolated cardiac perfusion experimental method. Background Art

[0002] In the isolated heart perfusion experiment, the animal heart is removed, connected to a specific perfusion device, and perfused with perfusion fluid, eliminating neural and fluid control. Special analysis software is used to record ventricular intraventricular pressure, arterial blood pressure, and electrocardiogram signals, and automatically analyze various physiological parameters. It is used for studies on cardiac function and hemodynamic changes under pathophysiological conditions, and has been widely used in physiological, pathophysiological, and pharmacological research.

[0003] In existing heart perfusion experiments, the perfusion needle needs to be manually inserted into the aorta of the isolated heart, and then the heart is temporarily fixed on the perfusion needle with an artery clamp, and then the aorta is fixed on the perfusion needle. When the perfusion needle is manually inserted into the aorta, the hand may shake, resulting in inaccurate insertion position or damage to the aortic valve.

[0004] In addition, the existing perfusion device needs to fix the perfusion needle and the heart aorta together by ligating a rope after the perfusion needle is inserted into the heart aorta. This is difficult and time-consuming. Therefore, the preparation time before the perfusion process is too long, resulting in poor heart activity. Summary of the invention

[0005] The present invention provides an isolated heart perfusion experimental device and an isolated heart perfusion experimental method, aiming to solve the problems mentioned in the above background technology that when manually inserting the perfusion needle into the aorta, the hand may shake, resulting in inaccurate insertion position or damage to the aortic valve, and the existing perfusion device needs to fix the perfusion needle and the heart aorta together by ligating a rope after the perfusion needle is inserted into the heart aorta. The above-mentioned operations are relatively difficult and time-consuming. Therefore, the preparation time before the perfusion process is too long, resulting in poor heart activity.

[0006] The present invention is implemented as follows: an isolated heart perfusion experimental device comprises a base, a peristaltic pump is arranged on the top of the base, a support rod is fixedly connected to the top of the base, the number of the support rods is set to two, a support frame is fixedly connected to the top of the base, a receiving tube is installed above the base, a fixing mechanism is arranged inside the receiving tube, and the fixing mechanism is used to fix the isolated heart placed in the receiving tube;

[0007] An adjustment mechanism is provided between the two support rods and the accommodating tube, and the adjustment mechanism is used to adjust the position of the accommodating tube so that the aorta in the accommodating tube is adjusted to a vertical state;

[0008] A first electric push rod is fixedly connected to the bottom of the support frame, and a moving frame is fixedly connected to the output end of the first electric push rod. A fixed cylinder is arranged through the middle of the moving frame, and the fixed cylinder is fixedly connected to the moving frame. A delivery tube is arranged through the middle of the fixed cylinder, and a connecting tube is arranged between the input end of the delivery tube and the peristaltic pump, and an injection mechanism is arranged at the output end of the delivery tube, and the injection mechanism is used to inject experimental liquid for perfusion into the aorta of the heart.

[0009] Preferably, the fixing mechanism comprises an annular airbag and a placing tube, the annular airbag is arranged inside the accommodating tube, one side of the annular airbag is connected with an air intake pipe, the air intake pipe runs through the accommodating tube, the end of the air intake pipe is connected with a pressing airbag, a safety valve is arranged in the middle of the air intake pipe, and an air release valve is arranged in the middle of the air intake pipe;

[0010] The inner side of the annular airbag is wrapped to form a placement chamber, in which a placement tube with an upward opening is installed, the outer periphery of the top of the placement tube is fixedly connected to the top of the accommodation tube, and the placement tube is made of a deformable material;

[0011] The removed heart is placed in a placement tube with the aorta facing upwards, and gas can be filled into the annular airbag through the air inlet tube by pressing the airbag, so that the annular airbag is deformed and squeezes the placement tube, thereby squeezing the placement tube and squeezing the heart, so that the blood in the heart can be squeezed out to avoid blood coagulation in the heart, and the heart is fixed at the same time;

[0012] The setting of the safety valve can release the gas when the air pressure in the annular airbag is too high, thereby preventing the heart from being squeezed and damaged due to excessive air pressure in the annular airbag. When the heart needs to be removed, the pressure relief valve can be opened to shrink the annular airbag, thereby facilitating the removal of the heart.

[0013] Preferably, the outer periphery of the top of the placing tube is fixedly connected to the top of the accommodating tube, the top of the placing tube expands outward to form a snap-fit ​​portion, the top of the accommodating tube is located in the snap-fit ​​portion, and the top of the accommodating tube is fixedly connected to the inner wall of the snap-fit ​​portion.

[0014] Preferably, the adjustment mechanism comprises a moving frame, with respect to the lateral direction of the moving frame, the moving frame is arranged between two support rods, one side of the moving frame is fixedly connected to a first sliding rod, the other side of the moving frame is rotatably connected to a first threaded rod via a bearing, the middle portion of the first sliding rod passes through one of the support rods and is slidably connected thereto, the middle portion of the first threaded rod is threadedly connected to the other support rod, and the end portion of the first threaded rod is fixedly connected to a first knob;

[0015] With respect to the longitudinal direction of the moving frame, a mounting piece is provided in the middle of the moving frame, a second sliding rod is fixedly connected to one side of the mounting piece, a second threaded rod is rotatably connected to the other side of the mounting piece through a bearing, a middle portion of the second sliding rod is slidably connected to the moving frame, a middle portion of the second threaded rod passes through the moving frame and is threadedly connected to the moving frame, and a second knob is fixedly connected to the end of the second threaded rod;

[0016] The mounting piece is in an annular shape, a universal ball is rollingly connected inside the mounting piece, a connecting rod is fixedly connected to the top of the universal ball, the top of the connecting rod is fixedly connected to the accommodating cylinder, and a pull rod is fixedly connected to the bottom of the universal ball;

[0017] By rotating the first threaded rod through the first knob, the first threaded rod can be driven to move relative to the support rod threadedly connected thereto, and at the same time, the first threaded rod will drive the moving frame to move along the axis direction of the first sliding rod, thereby adjusting the lateral position of the placement tube connected to the moving frame;

[0018] Rotating the second threaded rod by the second knob can drive the second threaded rod to move relative to the movable frame threadedly connected thereto. At the same time, the second threaded rod will drive the mounting piece to move along the axial direction of the second sliding rod, thereby adjusting the longitudinal position of the placement tube connected to the mounting piece.

[0019] Preferably, the injection mechanism includes an expansion structure and a limiting structure, the expansion structure is used to actively expand the heart, thereby facilitating the insertion of the output end of the delivery tube into the heart's aorta, and the limiting structure is used to fix the output end of the delivery tube in the heart's aorta.

[0020] Preferably, the expansion structure comprises a second electric push rod, the number of the second electric push rods is set to two, the tops of the two second electric push rods are fixedly connected to the moving frame, the output ends of the two second electric push rods are fixedly connected to the same first moving plate, a first through groove is opened in the middle of the first moving plate, a connecting ring is provided at the bottom of the first moving plate, and a plurality of first fixing rods are fixedly connected between the first moving plate and the connecting ring;

[0021] An expansion rod is hingedly provided on the outer periphery of the connecting ring, the expansion rod is L-shaped, a groove is provided at one end of the top of the expansion rod, a limiting axis is fixedly connected to the inner wall of the groove, and the number of the expansion rods is set to be multiple;

[0022] Two third electric push rods are fixedly connected to the bottom of the first movable plate, the output ends of the two third electric push rods are fixedly connected to the same second movable plate, the middle parts of the plurality of first fixed rods are all arranged through the second movable plate, and the middle parts of the plurality of first fixed rods are all slidably connected to the second movable plate;

[0023] A second through slot is provided in the middle of the second movable plate, a plurality of second fixing rods are fixedly connected to the bottom of the second movable plate, the bottoms of the plurality of second fixing rods are fixedly connected to limit frames, and the plurality of limit frames are movably sleeved on the outer peripheries of the plurality of corresponding limit shafts;

[0024] In the initial state, the multiple expansion rods are all in an inclined state, and the ends of the multiple expansion rods are combined into a tip by abutting against each other. By starting the first electric push rod to extend, the moving frame can be driven to move downward. At this time, the ends of the multiple expansion rods connected to the moving frame are all inserted into the aorta of the heart.

[0025] Then, the two third electric push rods are started to contract at the same time, which can drive the second movable plate to move upward. At this time, the second movable plate will drive the multiple limit frames to move upward synchronously through the multiple second fixed rods at the bottom. At the same time, the multiple limit frames will drive the multiple expansion rods to rotate around the hinge point as the axis through the multiple limit shafts, thereby making the multiple expansion rods open and expand the aorta;

[0026] Afterwards, by starting the first electric push rod to continue to extend, the movable frame and the end of the delivery tube connected to the movable frame can be driven to be inserted into the aorta, and then the two second electric push rods are simultaneously started to contract and drive the first movable plate to move upward. At the same time, the first movable plate will drive the connecting ring and multiple expansion rods to move upward through multiple first fixed rods, thereby separating the multiple expansion rods from the heart's aorta.

[0027] Preferably, the limiting structure comprises an annular bin, a leakage hole is provided on the top of the annular bin, the bottom of the annular bin is conical, the annular bin is fixedly sleeved on the outer periphery of the end of the delivery pipe, an annular groove is provided on the outer periphery of the annular bin, a limiting ring is fixedly connected to the inner wall of the annular bin, a piston ring is provided above the limiting ring, two push rods are fixedly connected to the top of the piston ring, the middle parts of the two push rods both penetrate the annular bin, the two push rods are slidably connected to the annular bin, the tops of the two push rods are fixedly connected to the same push ring, and the outer peripheries of the two push rods are provided with clamping parts;

[0028] A first return spring is arranged between the piston ring and the limiting ring, the top of the first return spring abuts against the piston ring, and the bottom of the first return spring abuts against the limiting ring.

[0029] Preferably, the clamping component comprises a clamping slot and a fixing block, and the clamping slot is provided on the surface of the abutment rod;

[0030] The bottom of the fixed block is fixedly connected to the annular bin, a clamping frame is provided on the outer periphery of the fixed block, the clamping frame is sleeved on the outer periphery of the support rod, a clamping block is fixedly connected to one side of the inner wall of the clamping frame, and a third sliding rod is fixedly connected to the other side of the inner wall of the clamping frame;

[0031] The clamping block matches the clamping slot, the middle part of the third sliding rod is slidably connected with the fixed block, the outer periphery of the third sliding rod is slidably sleeved with a second return spring, one end of the second return spring abuts against the fixed block, and the other end of the second return spring abuts against the clamping frame;

[0032] When the second movable plate moves downward, it will squeeze the butt ring downward, and at the same time, the butt ring will drive the piston ring to move downward in the annular bin through the two butt rods. At the same time, the bottom of the piston ring will squeeze and compress the reset spring. At the same time, when the slots on the surfaces of the two butt rods correspond to the positions of the blocks on the surface of the clamping frames, the two second reset springs will squeeze and drive the two clamping frames to move, and at the same time, the two clamping frames will drive the two blocks to clamp with the slots on the surfaces of the two butt rods respectively, thereby limiting the two butt rods.

[0033] When the delivery tube drives the annular bin at the end to be inserted into the aorta of the heart, the multiple expansion rods are first lifted upward so that the annular bin is against the inner wall of the aorta of the heart, and then the multiple expansion rods are opened and moved downward, and finally the multiple expansion rods are contracted. At this time, the multiple expansion rods can squeeze the outer sides of the two clamping frames, so that the two clamping frames respectively drive the two clamping blocks to disengage from the two clamping grooves, and at this time, the piston ring can be driven to move upward in the annular bin through the first return spring, so that the bottom of the piston ring and the annular bin are in a negative pressure state, and the annular groove on the outer periphery of the annular bin is adsorbed to the inner wall of the aorta, thereby adsorbing and fixing the aorta of the heart and the annular bin on the outer periphery of the insertion tube.

[0034] The present invention provides an isolated heart perfusion experiment method, comprising the following steps:

[0035] S1. Anesthetize the rats. Turn the rats over with their abdomen facing upwards, insert a needle into the lower abdomen on the left or right side, and inject 1.5% sodium pentobarbital solution intraperitoneally. After waiting for 5 minutes, squeeze the rat's toes or tail to see if it loses its reaction, so as to confirm whether the rat has entered a state of deep anesthesia.

[0036] S2. The heart is removed, the abdominal cavity of the rat is cut open, the ribs of the rat are turned over and fixed with hemostats to expose the heart of the rat, then the pericardium is cut, the superior and inferior vena cava, pulmonary artery, aorta and tissues around the heart are cut, the heart is removed and immediately placed in a placement tube containing perfusion fluid.

[0037] S3. Cardiac cannulation is performed so that the aorta of the heart placed in the placement tube faces upwards, and the pressing balloon is pressed to expand the annular balloon and squeeze the placement tube, thereby deforming the placement tube and squeezing the removed heart, thereby squeezing the blood in the heart to prevent the blood from clotting inside the heart and fix the heart at the same time.

[0038] S4. Heart positioning: the position and angle of the placement tube and the fixed heart are adjusted by the adjustment mechanism so that the aorta of the heart is aligned with the bottom end of the delivery tube.

[0039] S5. Cardiac perfusion: Move the delivery tube downward so that the expansion structure at the end of the delivery tube is inserted into the aorta and expands the aorta. Then insert the end of the delivery tube into the aorta, contract the expansion structure and lift the expansion structure upward. Then, fix the inner wall of the aorta to the periphery of the delivery tube through the limiting structure on the periphery of the delivery tube, and finally use the peristaltic pump to perfuse the perfusion fluid into the heart at a constant rate.

[0040] Compared with the prior art, the present invention has the following beneficial effects: an isolated heart perfusion experimental device and an isolated heart perfusion experimental method of the present invention:

[0041] 1. The fixing mechanism can squeeze out the blood in the isolated heart to prevent blood coagulation in the heart, and fix the heart at the same time. The adjustment mechanism can adjust the horizontal position, longitudinal position and deflection angle of the fixed heart, so that the aorta of the heart can be aligned with the perfusion delivery tube, which is convenient for subsequent perfusion of the heart.

[0042] 2. By setting up an injection mechanism, the heart's aorta can be expanded, thereby facilitating the insertion of the delivery tube into the aorta, which is beneficial to improving the efficiency of inserting the delivery tube into the heart's aorta. In combination with the limiting structure at the end of the delivery tube, the inner wall of the heart's aorta can be adsorbed and fixed to the outside of the delivery tube, so that there is no need to set up additional ligatures to ligate the heart's aorta, which is beneficial to further improve the perfusion efficiency of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0044] Figure 2 It is a schematic diagram of the adjustment mechanism of the present invention;

[0045] Figure 3 is a cross-sectional view of the fixing mechanism of the present invention;

[0046] Figure 4 It is a schematic diagram of the injection mechanism of the present invention;

[0047] Figure 5 It is a partial schematic diagram of the expansion structure of the present invention;

[0048] Figure 6 It is a schematic diagram of the connection position between the limit frame and the second fixing rod of the present invention;

[0049] Figure 7 It is a cross-sectional view of the limiting structure of the present invention;

[0050] Figure 8 It is a schematic diagram of the installation position of the support rod of the present invention;

[0051] Fig. 9 For the present invention Figure 1 A magnified view of the structure of part A;

[0052] Fig.10 For the present invention Figure 2 An enlarged view of the structure of part B;

[0053] Fig.11 For the present invention Figure 7 Enlarged view of the C part structure.

[0054] In the figure:

[0055] 1. Base; 11. Peristaltic pump; 12. Support rod; 13. Support frame; 14. Accommodating cylinder; 15. First electric push rod; 16. Moving frame; 17. Fixed cylinder; 18. Delivery pipe; 19. Connecting pipe; 2. Fixing mechanism; 21. Annular airbag; 22. Placement cylinder; 23. Intake pipe; 24. Pressing airbag; 25. Safety valve; 26. Deflation valve; 3. Adjusting mechanism; 31. Moving frame; 32. First slide rod; 33. First threaded rod; 34. First knob; 35. Mounting piece; 36. Second slide rod; 37. Second threaded rod; 38. Second knob; 39. Universal ball; 310. Connecting rod; 311. Pull rod; 4. Injection mechanism; Expansion structure; 411. Second electric push rod; 412. The first movable plate; 413, the first through groove; 414, the connecting ring; 415, the first fixed rod; 416, the expansion rod; 417, the groove; 418, the limiting shaft; 419, the third electric push rod; 4110, the second movable plate; 4111, the second through groove; 4112, the second fixed rod; 4113, the limiting frame; the limiting structure; 421, the annular bin; 422, the air leakage hole; 423, the annular groove; 424, the limiting ring; 425, the piston ring; 426, the push rod; 427, the push ring; 428, the clamping component; 429, the first return spring; 4281, the clamping groove; 4282, the fixed block; 4283, the clamping frame; 4284, the clamping block; 4285, the third slide rod; 4286, the second return spring. DETAILED DESCRIPTION

[0056] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0057] The components of the embodiments of the present invention generally described and shown in the drawings herein may be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0058] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0059] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0060] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0061] See also Figure 1-Figure 11 The present invention provides a technical solution: an isolated heart perfusion experimental device, comprising a base 1, a peristaltic pump 11 is arranged on the top of the base 1, a support rod 12 is fixedly connected to the top of the base 1, the number of the support rods 12 is set to two, a support frame 13 is fixedly connected to the top of the base 1, a receiving tube 14 is installed above the base 1, a fixing mechanism 2 is arranged inside the receiving tube 14, and the fixing mechanism 2 is used to fix the isolated heart placed in the receiving tube 14;

[0062] An adjustment mechanism 3 is provided between the two support rods 12 and the accommodating tube 14, and the adjustment mechanism 3 is used to adjust the position of the accommodating tube 14 so that the aorta in the accommodating tube 14 is adjusted to a vertical state;

[0063] A first electric push rod 15 is fixedly connected to the bottom of the support frame 13, and a mobile frame 16 is fixedly connected to the output end of the first electric push rod 15. A fixed cylinder 17 is arranged through the middle of the mobile frame 16, and the fixed cylinder 17 is fixedly connected to the mobile frame 16. A delivery tube 18 is arranged through the middle of the fixed cylinder 17, and a connecting tube 19 is arranged between the input end of the delivery tube 18 and the peristaltic pump 11. An injection mechanism 4 is arranged at the output end of the delivery tube 18, and the injection mechanism 4 is used to inject the experimental liquid for perfusion into the aorta of the heart;

[0064] In the present application, a fixing mechanism 2 is provided, which is used to fix the isolated heart placed in the containing tube 14. At the same time, the posture of the containing tube 14 can be adjusted by the adjusting mechanism 3, so that the aorta of the isolated heart in the containing tube 14 is aligned with the bottom end of the delivery tube 18. Through such measures, mechanical fixation and focusing are utilized, so that when the perfusion needle is inserted into the aorta manually, the hand may shake, thereby improving the insertion position accuracy or reducing the possibility of damaging the aortic valve.

[0065] Further, the fixing mechanism 2 includes an annular airbag 21 and a placing tube 22. The annular airbag 21 is arranged inside the accommodating tube 14. An air intake pipe 23 is arranged on one side of the annular airbag 21. The air intake pipe 23 penetrates the accommodating tube 14. A pressing airbag 24 is arranged on the end of the air intake pipe 23. A safety valve 25 is arranged in the middle of the air intake pipe 23. An air release valve 26 is arranged in the middle of the air intake pipe 23.

[0066] The inner side of the annular airbag 21 is wrapped to form a placement chamber, in which a placement tube 22 with an upward opening is installed, and the outer periphery of the top of the placement tube 22 is fixedly connected to the top of the accommodating tube 14, and the placement tube 22 is made of deformable material;

[0067] The inner side of the annular airbag 21 is wrapped to form a placement chamber, in which a placement tube 22 with an upward opening is installed, and the outer periphery of the top of the placement tube 22 is fixedly connected to the top of the accommodating tube 14, and the placement tube 22 is made of deformable material;

[0068] When in use, the removed heart's aorta is placed facing upward in the placement tube 22, and gas can be filled into the annular airbag 21 through the air inlet pipe 23 by pressing the airbag 24. Due to the limitation of the inner wall of the containing tube 14, the annular airbag 21 can only expand inward, and the expanded annular airbag 21 deforms and squeezes the placement tube 22, thereby causing the placement tube 22 to deform and squeeze the heart, so that the blood in the heart can be squeezed out to avoid blood coagulation in the heart, and the heart can be fixed at the same time.

[0069] In the present application, it is not necessary for the entire placement tube 22 to be made of a deformable material. In practice, only the area where the placement tube 22 is placed on the heart is made of a deformable material. The inflated annular airbag 21 deforms and squeezes the placement tube 22, thereby causing the placement tube 22 to deform and squeeze the heart, thereby squeezing out the blood in the heart to prevent the blood in the heart from clotting and fix the heart at the same time. Figure 3 It can be seen that the outer periphery of the top of the placing tube 22 is fixedly connected to the top of the accommodating tube 14, and the top of the placing tube 22 expands outward to form a snap-on portion, while the top of the accommodating tube 14 is located in the snap-on portion and is fixedly connected to the inner wall of the snap-on portion. At this time, the snap-on portion is understood to be a hard material; of course, if the placing tube 22 is made of deformable material as a whole, then the snap-on portion can be directly fixedly connected to the top of the accommodating tube 14 at this time.

[0070] In some applications, the deformable material may be made of soft silicone material.

[0071] Furthermore, the outer periphery of the top of the placement tube 22 is fixedly connected to the top of the accommodating tube 14, the top of the placement tube 22 expands outward to form a snap-fit ​​portion, the top of the accommodating tube 14 is located in the snap-fit ​​portion, and the top of the accommodating tube 14 is fixedly connected to the inner wall of the snap-fit ​​portion.

[0072] Further, the adjustment mechanism 3 includes a moving frame 31. With respect to the lateral direction of the moving frame 31, the moving frame 31 is arranged between the two support rods 12. A first sliding rod 32 is fixedly connected to one side of the moving frame 31. A first threaded rod 33 is rotatably connected to the other side of the moving frame 31 through a bearing. The middle portion of the first sliding rod 32 passes through one of the support rods 12 and is slidably connected thereto. The middle portion of the first threaded rod 33 is threadedly connected to the other support rod 12. A first knob 34 is fixedly connected to the end of the first threaded rod 33.

[0073] With respect to the longitudinal direction of the moving frame 31, a mounting member 35 is provided in the middle of the moving frame 31, a second sliding rod 36 is fixedly connected to one side of the mounting member 35, a second threaded rod 37 is rotatably connected to the other side of the mounting member 35 through a bearing, a middle portion of the second sliding rod 36 penetrates the moving frame 31 and is slidably connected thereto, a middle portion of the second threaded rod 37 is threadedly connected to the moving frame 31, and a second knob 38 is fixedly connected to the end of the second threaded rod 37;

[0074] The mounting member 35 is annular, and a universal ball 39 is rollingly connected inside the mounting member 35. A connecting rod 310 is fixedly connected to the top of the universal ball 39. The top of the connecting rod 310 is fixedly connected to the accommodating tube 14. A pull rod 311 is fixedly connected to the bottom of the universal ball 39.

[0075] When in use, the first threaded rod 33 can be driven to move relative to the support rod 12 threadedly connected thereto by rotating the first knob 34, and the first threaded rod 33 can drive the moving frame 31 to move along the axis direction of the first slide bar 32, thereby adjusting the lateral position of the placement tube 22 connected to the moving frame 31;

[0076] By rotating the second threaded rod 37 through the second knob 38, the second threaded rod 37 can be driven to move relative to the moving frame 31 threadably connected thereto, and the second threaded rod 37 can drive the mounting member 35 to move along the axis direction of the second slide rod 36, thereby adjusting the longitudinal position of the placement tube 22 connected to the mounting member 35;

[0077] The universal ball 39 can be moved arbitrarily by pulling, thereby driving the heart in the accommodating tube 14 to deflect in any direction through the connecting rod 310, and then the aorta of the heart can be adjusted to a vertical state;

[0078] A rubber layer is disposed on the outer periphery of the universal ball 39 to increase the damping between the universal ball 39 and the mounting member 35 , so that the universal ball 39 can be fixed at any position by friction after moving.

[0079] Furthermore, the injection mechanism 4 includes an expansion structure 41 and a limiting structure 42. The expansion structure 41 is used to actively expand the heart, thereby facilitating the insertion of the output end of the delivery tube 18 into the heart's aorta, and the limiting structure 42 is used to fix the output end of the delivery tube 18 in the heart's aorta.

[0080] Further, the expansion structure 41 includes a second electric push rod 411, the number of the second electric push rod 411 is set to two, the tops of the two second electric push rods 411 are fixedly connected to the moving frame 16, the output ends of the two second electric push rods 411 are fixedly connected to the same first moving plate 412, the middle of the first moving plate 412 is provided with a first through groove 413, the bottom of the first moving plate 412 is provided with a connecting ring 414, and a plurality of first fixing rods 415 are fixedly connected between the first moving plate 412 and the connecting ring 414;

[0081] An expansion rod 416 is hingedly provided on the outer periphery of the connecting ring 414, and the expansion rod 416 is set to be L-shaped. A groove 417 is opened at one end of the top of the expansion rod 416, and the inner wall of the groove 417 is fixedly connected to the limiting axis 418, and the number of the expansion rods 416 is set to be multiple; two third electric push rods 419 are fixedly connected to the bottom of the first movable plate 412, and the output ends of the two third electric push rods 419 are fixedly connected to the same second movable plate 4110, and the middle parts of the plurality of first fixed rods 415 are all arranged through the second movable plate 4110, and the middle parts of the plurality of first fixed rods 415 are all slidably connected to the second movable plate 4110; a second through groove 4111 is opened in the middle part of the second movable plate 4110, and the bottom of the second movable plate 4110 is fixedly connected to a plurality of second fixed rods 4112, and the bottoms of the plurality of second fixed rods 4112 are fixedly connected to a limiting frame 4113, and the plurality of limiting frames 4113 are respectively movably sleeved on the outer periphery of the corresponding limiting axis 418.

[0082] Furthermore, the limiting structure 42 includes an annular bin 421, a leakage hole 422 is provided on the top of the annular bin 421, the bottom of the annular bin 421 is conical, the annular bin 421 is fixedly sleeved on the outer periphery of the end of the conveying pipe 18, an annular groove 423 is provided on the outer periphery of the annular bin 421, a limiting ring 424 is fixedly connected to the inner wall of the annular bin 421, a piston ring 425 is provided above the limiting ring 424, two push rods 426 are fixedly connected to the top of the piston ring 425, the middle parts of the two push rods 426 both penetrate the annular bin 421, the two push rods 426 are both slidably connected to the annular bin 421, the tops of the two push rods 426 are fixedly connected to the same push ring 427, and the outer peripheries of the two push rods 426 are provided with clamping parts 428; a first return spring 429 is provided between the piston ring 425 and the limiting ring 424, the top of the first return spring 429 is against the piston ring 425, and the bottom of the first return spring 429 is against the limiting ring 424.

[0083] Furthermore, the clamping member 428 includes a clamping slot 4281 and a fixing block 4282 , and the clamping slot 4281 is formed on the surface of the supporting rod 426 ;

[0084] The bottom of the fixed block 4282 is fixedly connected to the annular bin 421, and a clamping frame 4283 is provided on the outer periphery of the fixed block 4282. The clamping frame 4283 is sleeved on the outer periphery of the support rod 426. A clamping block 4284 is fixedly connected to one side of the inner wall of the clamping frame 4283, and a third sliding rod 4285 is fixedly connected to the other side of the inner wall of the clamping frame 4283.

[0085] The clamping block 4284 matches the clamping slot 4281, the middle part of the third sliding rod 4285 is slidably connected with the fixed block 4282, and the outer periphery of the third sliding rod 4285 is slidably sleeved with a second return spring 4286, one end of the second return spring 4286 abuts against the fixed block 4282, and the other end of the second return spring 4286 abuts against the clamping frame 4283;

[0086] The present invention provides an isolated heart perfusion experiment method, comprising the following steps:

[0087] S1. Anesthetize the rats. Turn the rats over with their abdomen facing upwards, insert a needle into the lower abdomen on the left or right side, and inject 1.5% sodium pentobarbital solution intraperitoneally. After waiting for 5 minutes, squeeze the rat's toes or tail to see if it loses its reaction, so as to confirm whether the rat has entered a state of deep anesthesia.

[0088] S2. The heart is taken out, the abdominal cavity of the rat is cut open, the ribs of the rat are turned over and fixed with hemostatic forceps to expose the heart of the rat, and then the pericardium is cut, the superior and inferior vena cava, pulmonary artery, aorta and tissues around the heart are cut, and the heart is removed and immediately placed in the placement tube 22 filled with perfusion fluid.

[0089] S3. Cardiac cannulation is performed so that the aorta of the heart placed in the placement tube 22 faces upwards, and the pressing balloon 24 is pressed to expand the annular balloon 21 and squeeze the placement tube 22, thereby deforming the placement tube 22 and squeezing the removed heart, thereby squeezing the blood in the heart to prevent the blood from clotting inside the heart and fix the heart at the same time.

[0090] S4. Positioning the heart: adjusting the position and angle of the placement tube 22 and the fixed heart through the adjustment mechanism 3 so that the aorta of the heart is aligned with the bottom end of the delivery tube 18.

[0091] S5. Cardiac perfusion, move the delivery tube 18 downward so that the expansion structure 41 at the end of the delivery tube 18 is inserted into the aorta and the aorta is expanded, then the end of the delivery tube 18 is inserted into the aorta, and then the expansion structure 41 is contracted and lifted upward, and then the inner wall of the aorta is fixed to the outer periphery of the delivery tube 18 by the limiting structure 42 on the periphery of the delivery tube 18, and finally the perfusion fluid is perfused into the heart at a constant rate by the peristaltic pump 11.

[0092] The working principle and use process of the present invention:

[0093] First, place the removed heart with its aorta facing upward in the placement tube 22, and fill the annular airbag 21 with gas through the air inlet tube 23 by pressing the airbag, so that the annular airbag 21 is deformed and squeezes the placement tube 22, thereby making the placement tube 22 deformed and squeezes the heart, so that the blood in the heart can be squeezed out to avoid blood coagulation in the heart, and the heart can be fixed at the same time;

[0094] By rotating the first threaded rod 33 through the first knob 34, the first threaded rod 33 can be driven to move relative to the support rod 12 threadedly connected thereto, and the first threaded rod 33 will drive the moving frame 31 to move along the axis direction of the first slide rod 32, thereby adjusting the lateral position of the placement tube 22 connected to the moving frame 31;

[0095] By rotating the second threaded rod 37 through the second knob 38, the second threaded rod 37 can be driven to move relative to the moving frame 31 threadably connected thereto, and the second threaded rod 37 can drive the mounting member 35 to move along the axis direction of the second slide rod 36, thereby adjusting the longitudinal position of the placement tube 22 connected to the mounting member 35;

[0096] The universal ball 39 can be moved arbitrarily by pulling, thereby driving the heart in the accommodating tube 14 to deflect in any direction through the connecting rod 310, and then the aorta of the heart can be adjusted to a vertical state;

[0097] The aorta of the heart can be aligned with the bottom end of the delivery tube 18 in the above manner.

[0098] By starting the two third electric push rods 419 to contract, the second movable plate 4110 can be driven to move upward. At this time, the second movable plate 4110 will drive the multiple limit frames 4113 to move upward synchronously through the multiple second fixed rods 4112 at the bottom. At the same time, the multiple limit frames 4113 will drive the multiple expansion rods 416 to rotate around the hinge point as the axis through the multiple limit shafts 418, so that the multiple expansion rods 416 can be opened and the aorta can be expanded. When the two third electric push rods 419 are started to extend at the same time, the multiple expansion rods 416 can be contracted through the above structure.

[0099] By starting the two second electric push rods 411 to extend, the first movable plate 412 can be driven to move downward. At the same time, the bottom of the first movable plate 412 can drive the multiple connecting rings 414 and the multiple expansion rods 416 to move downward through the multiple first fixing rods 415. Therefore, when the second electric push rods 411 are retracted, the multiple expansion rods 416 can be driven to move upward.

[0100] First, when the multiple expansion rods 416 are in the expanded state, the two third electric push rods 419 are started to extend at the same time, and drive the second moving plate 4110 to move downward. When the second moving plate 4110 moves downward, it will squeeze the ring 427 downward. At the same time, the ring 427 will drive the piston ring 425 to move downward in the annular warehouse 421 through the two push rods 426. At the same time, the bottom of the piston ring 425 will squeeze and compress the return spring. At the same time, when the grooves 4281 on the surfaces of the two push rods 426 correspond to the positions of the blocks 4284 on the surface of the clamping frame 4283, the two second return springs 4286 will squeeze and drive the two clamping frames 4283 to move. At the same time, the two clamping frames 4283 will drive the two blocks 4284 to clamp with the grooves 4281 on the surfaces of the two push rods 426 respectively, thereby limiting the two push rods 426.

[0101] Then, the multiple expansion rods 416 are contracted so that the ends of the multiple expansion rods 416 are abutted against each other to form a tip. Then, the first electric push rod 15 is started to extend, which can drive the moving frame 16 to move downward. At this time, the ends of the multiple expansion rods 416 connected to the moving frame 16 are inserted into the aorta of the heart. Then, the multiple expansion rods 416 are opened to expand the aorta of the heart.

[0102] Then, the first electric push rod 15 is started to extend further to drive the moving frame 16 and the annular bin 421 at the end of the delivery tube 18 connected to the moving frame 16 to be inserted into the aorta, and then the plurality of expansion rods 416 are moved upward and separated from the aorta, and at this time, the annular bin 421 is against the inner wall of the aorta;

[0103] When the positions of the plurality of expansion rods 416 correspond to the positions of the clamping frames 4283, the plurality of expansion rods 416 are contracted, and the plurality of expansion rods 416 can squeeze the outer sides of the two clamping frames 4283, so that the two clamping frames 4283 respectively drive the two clamping blocks 4284 to disengage from the two clamping grooves 4281, and the first return spring 429 can drive the piston ring 425 to move upward in the annular chamber 421, so that a negative pressure state is formed between the bottom of the piston ring 425 and the annular chamber 421, so that the annular groove 423 on the outer periphery of the annular chamber 421 is adsorbed to the inner wall of the aorta, so that the aorta of the heart is adsorbed and fixed to the annular chamber 421 on the outer periphery of the insertion tube;

[0104] At this time, by starting the peristaltic pump 11, the perfusion fluid can be perfused to the heart at a constant rate through the connecting tube 19 and the delivery tube 18.

[0105] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An isolated heart perfusion experimental device, comprising a base (1), characterized in that: A peristaltic pump (11) is provided on the top of the base (1), a support rod (12) is fixedly connected to the top of the base (1), and the number of the support rods (12) is set to two. A support frame (13) is also fixedly connected to the top of the base (1), and a receiving tube (14) is installed above the base (1), and a fixing mechanism (2) is provided inside the receiving tube (14), and the fixing mechanism (2) is used to fix the isolated heart placed in the receiving tube (14); An adjustment mechanism (3) is provided between the two support rods (12) and the accommodating cylinder (14), and the adjustment mechanism (3) is used to adjust the position of the accommodating cylinder (14) so ​​that the heart aorta in the accommodating cylinder (14) is adjusted to a vertical state; A first electric push rod (15) is fixedly connected to the bottom of the support frame (13); an output end of the first electric push rod (15) is fixedly connected to a moving frame (16); a fixed cylinder (17) is arranged through the middle of the moving frame (16); the fixed cylinder (17) is fixedly connected to the moving frame (16); a delivery tube (18) is arranged through the middle of the fixed cylinder (17); a connecting tube (19) is arranged between the input end of the delivery tube (18) and the peristaltic pump (11); an injection mechanism (4) is arranged at the output end of the delivery tube (18); the injection mechanism (4) is used to inject a perfusion experimental liquid into the aorta of the heart.

2. An isolated heart perfusion experimental device as claimed in claim 1, characterized in that: The fixing mechanism (2) comprises an annular airbag (21) and a placement tube (22), the annular airbag (21) being arranged inside the accommodating tube (14), an air intake pipe (23) being arranged on one side of the annular airbag (21), the air intake pipe (23) being arranged through the accommodating tube (14), a pressing airbag (24) being arranged at the end of the air intake pipe (23), a safety valve (25) being arranged in the middle of the air intake pipe (23), and an air release valve (26) being arranged in the middle of the air intake pipe (23); The inner side of the annular airbag (21) is wrapped to form a placement chamber, in which a placement tube (22) with an upward opening is installed, the top periphery of the placement tube (22) is fixedly connected to the top of the accommodating tube (14), and the placement tube (22) is made of deformable material.

3. An isolated heart perfusion experimental device as claimed in claim 1, characterized in that: The outer periphery of the top of the placement tube (22) is fixedly connected to the top of the accommodating tube (14); the top of the placement tube (22) expands outward to form a buckle portion; the top of the accommodating tube (14) is located in the buckle portion; and the top of the accommodating tube (14) is fixedly connected to the inner wall of the buckle portion.

4. An isolated heart perfusion experimental device as claimed in claim 1, characterized in that: The adjustment mechanism (3) comprises a moving frame (31). With respect to the transverse direction of the moving frame (31), the moving frame (31) is arranged between two support rods (12). A first sliding rod (32) is fixedly connected to one side of the moving frame (31). A first threaded rod (33) is rotatably connected to the other side of the moving frame (31) via a bearing. The middle part of the first sliding rod (32) passes through one of the support rods (12) and is slidably connected thereto. The middle part of the first threaded rod (33) is threadedly connected to the other support rod (12). The end of the first threaded rod (33) is fixedly connected to a first knob (34). In the longitudinal direction of the moving frame (31), a mounting member (35) is provided in the middle of the moving frame (31), one side of the mounting member (35) is fixedly connected to a second sliding rod (36), and the other side of the mounting member (35) is rotatably connected to a second threaded rod (37) through a bearing, the middle part of the second sliding rod (36) passes through the moving frame (31) and is slidably connected thereto, the middle part of the second threaded rod (37) is threadedly connected to the moving frame (31), and the end of the second threaded rod (37) is fixedly connected to a second knob (38); The mounting member (35) is configured in a ring shape, and a universal ball (39) is rollingly connected inside the mounting member (35). A connecting rod (310) is fixedly connected to the top of the universal ball (39). The top of the connecting rod (310) is fixedly connected to the accommodating cylinder (14), and a pull rod (311) is fixedly connected to the bottom of the universal ball (39).

5. The isolated heart perfusion experimental device according to claim 1, characterized in that: The injection mechanism (4) comprises an expansion structure (41) and a limiting structure (42), wherein the expansion structure (41) is used to actively expand the heart, thereby facilitating the insertion of the output end of the delivery tube (18) into the heart's aorta, and the limiting structure (42) is used to fix the output end of the delivery tube (18) in the heart's aorta.

6. An isolated heart perfusion experimental device as claimed in claim 4, characterized in that: The expansion structure (41) comprises a second electric push rod (411), the number of the second electric push rods (411) is set to two, the tops of the two second electric push rods (411) are fixedly connected to the moving frame (16), the output ends of the two second electric push rods (411) are fixedly connected to the same first moving plate (412), a first through groove (413) is opened in the middle of the first moving plate (412), a connecting ring (414) is provided at the bottom of the first moving plate (412), and a plurality of first fixing rods (415) are fixedly connected between the first moving plate (412) and the connecting ring (414); An expansion rod (416) is hingedly provided on the outer periphery of the connection ring (414), the expansion rod (416) is set to be L-shaped, a groove (417) is opened at one end of the top of the expansion rod (416), and a limiting axis (418) is fixedly connected to the inner wall of the groove (417), and the number of the expansion rods (416) is set to be multiple; Two third electric push rods (419) are fixedly connected to the bottom of the first movable plate (412); the output ends of the two third electric push rods (419) are fixedly connected to the same second movable plate (4110); the middle parts of the plurality of first fixed rods (415) are all arranged to penetrate the second movable plate (4110); and the middle parts of the plurality of first fixed rods (415) are all slidably connected to the second movable plate (4110); A second through slot (4111) is provided in the middle of the second movable plate (4110); a plurality of second fixed rods (4112) are fixedly connected to the bottom of the second movable plate (4110); the bottoms of the plurality of second fixed rods (4112) are fixedly connected to a limiting frame (4113); and the plurality of limiting frames (4113) are movably sleeved on the outer periphery of the corresponding limiting shaft (418).

7. An isolated heart perfusion experimental device as claimed in claim 4, characterized in that: The limiting structure (42) comprises an annular bin (421), a leak hole (422) is provided at the top of the annular bin (421), the bottom of the annular bin (421) is conical, the annular bin (421) is fixedly sleeved on the outer periphery of the end of the delivery pipe (18), an annular groove (423) is provided at the outer periphery of the annular bin (421), a limiting ring (424) is fixedly connected to the inner wall of the annular bin (421), a piston ring (425) is provided above the limiting ring (424), two push rods (426) are fixedly connected to the top of the piston ring (425), the middle parts of the two push rods (426) are both arranged through the annular bin (421), the two push rods (426) are both slidably connected to the annular bin (421), the tops of the two push rods (426) are fixedly connected to the same push ring (427), and the outer peripheries of the two push rods (426) are both provided with a clamping component (428); A first return spring (429) is provided between the piston ring (425) and the limiting ring (424); the top of the first return spring (429) abuts against the piston ring (425), and the bottom of the first return spring (429) abuts against the limiting ring (424).

8. An isolated heart perfusion experimental device as claimed in claim 6, characterized in that: The clamping component (428) comprises a clamping slot (4281) and a fixing block (4282); the clamping slot (4281) is formed on the surface of the support rod (426); The bottom of the fixed block (4282) is fixedly connected to the annular bin (421); a clamping frame (4283) is provided on the outer periphery of the fixed block (4282); the clamping frame (4283) is sleeved on the outer periphery of the support rod (426); a clamping block (4284) is fixedly connected to one side of the inner wall of the clamping frame (4283); and a third sliding rod (4285) is fixedly connected to the other side of the inner wall of the clamping frame (4283); The clamping block (4284) matches the clamping slot (4281), the middle part of the third sliding rod (4285) is slidably connected to the fixed block (4282), and the outer peripheral sliding sleeve of the third sliding rod (4285) is provided with a second return spring (4286), one end of the second return spring (4286) is against the fixed block (4282), and the other end of the second return spring (4286) is against the clamping frame (4283).

9. An isolated heart perfusion experiment method, characterized in that: The isolated heart perfusion experimental device according to any one of claims 1 to 8 further comprises: S1. Anesthetize the rats. Turn the rats over with their abdomen facing upwards, insert a needle into the lower abdomen on the left or right side, and inject 1.5% sodium pentobarbital solution intraperitoneally. After waiting for 5 minutes, squeeze the rats' toes or tails to see if they lose their reaction, so as to confirm whether the rats have entered a deep anesthesia state. S2. The heart is removed, the abdominal cavity of the rat is cut open, the ribs of the rat are turned over and fixed with a hemostatic clamp to expose the heart of the rat, and then the pericardium is cut, the superior and inferior vena cava, pulmonary artery, aorta and tissues around the heart are cut, and the heart is removed and immediately placed in a placement tube (22) filled with perfusion fluid; S3. The heart is cannulated, so that the aorta of the heart placed in the placement tube (22) faces upward, and the pressing balloon (24) is pressed to expand the annular balloon (21) and squeeze the placement tube (22), so that the placement tube (22) is deformed and squeezes the removed heart, so that the blood in the heart can be squeezed out to prevent the blood from clotting inside the heart, and the heart is fixed at the same time; S4. Positioning the heart, adjusting the position and angle of the placement tube (22) and the fixed heart by the adjustment mechanism (3), so that the aorta of the heart is aligned with the bottom end of the delivery tube (18); S5. Cardiac perfusion: Move the delivery tube (18) downward so that the expansion structure (41) at the end of the delivery tube (18) is inserted into the aorta and the aorta is expanded. Then, insert the end of the delivery tube (18) into the aorta, contract the expansion structure (41) and lift the expansion structure (41) upward, and then fix the inner wall of the aorta to the periphery of the delivery tube (18) through the limiting structure (42) on the periphery of the delivery tube (18). Finally, the perfusion fluid is perfused into the heart at a constant rate through the peristaltic pump (11).

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