Liquid crystal type oil-in-water pesticide preparation as well as preparation method and application thereof

Abstract

The invention discloses a liquid crystal type oil-in-water pesticide preparation as well as a preparation method and application thereof, the liquid crystal type oil-in-water pesticide preparation comprises the following components in percentage by mass: 1-15% of difenoconazole, 1-25% of pyraclostrobin, 4.8-15% of vegetable oil, 5-20% of polyol, 1-5% of an emulsifier, 1-5% of a co-emulsifier, 0.1-0.5% of a thickener and the balance of deionized water; according to the invention, the pesticide preparation product is endowed with excellent stability by matching the specific emulsifier and co-emulsifier, the physical stability and chemical stability of the preparation product can still be maintained after multiple freezing and thawing, and the preparation process is simple and safe, is safe to users and environment-friendly, and has an excellent control effect on grape powdery mildew in the field.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and specifically relates to a liquid crystal oil-in-water pesticide preparation and a preparation method and application thereof. Background Art

[0002] Although some substances lose the rigidity of solid substances in the molten state or after being dissolved by solvents, they gain the fluidity of liquid substances and retain the anisotropic ordered arrangement of some crystalline substance molecules, forming an intermediate state with partial properties of both crystals and liquids. The oriented ordered fluid that exists in the process of transformation from solid to liquid is liquid crystal, which can be divided into thermotropic liquid crystal and lyotropic liquid crystal.

[0003] At present, pesticide preparations with difenoconazole and pyraclostrobin as the main active ingredients are mainly suspension concentrates and microcapsule suspension concentrates, such as:

[0004] CN116369313A discloses a difenoconazole and pyraclostrobin compound suspension and a preparation method thereof, wherein difenoconazole and pyraclostrobin are compounded, supplemented with auxiliary agents such as a wetting dispersant, an antifreeze agent, a structure regulator, a thickener, a preservative, and a defoamer, and MK-5011, MK-8040, and BYK-2012 are used in combination as a wetting dispersant. However, the difenoconazole and pyraclostrobin compound suspension adopts wet grinding during the preparation process, and the grinding temperature needs to be controlled, resulting in high energy consumption during the production process.

[0005] CN107787994A discloses a pyraclostrobin-fenfenoconazole microcapsule suspension, which comprises the following components by mass percentage: pyraclostrobin: 1% to 20%; fenfenoconazole: 1% to 40%; capsule wall material: 6% to 10%; capsule core solvent: 15% to 20%; emulsifier: 1% to 5%; thickener: 0.1% to 1%; dispersant: 3% to 5%; antifreeze: 1% to 5%; preservative: 0.1% to 0.5%; pH regulator 1% to 3%; curing agent: 0.1% to 1%; the balance is water. However, the pyraclostrobin-fenfenoconazole microcapsule suspension usually uses formaldehyde aqueous solution as a curing agent during the preparation process, and formaldehyde has certain harm to human health.

[0006] In addition, compared with suspension concentrates and microcapsule suspension concentrates, when the active ingredients of pesticides form a liquid crystal structure, the absorption of the pesticide solution by plants can be further improved. Therefore, the development of water-in-oil pesticide formulations with liquid crystal structures has broad market prospects and is worthy of promotion and application in agricultural production. Summary of the invention

[0007] In view of the above-mentioned deficiencies in the prior art, the present invention discloses a liquid crystal type oil-in-water pesticide formulation and a preparation method and application thereof. The present invention uses difenoconazole and pyraclostrobin as active ingredients, and by matching specific emulsifiers and emulsifiers, a liquid crystal interface can be formed between the oil phase and the water phase to form a liquid crystal structure with a stable lattice, thereby giving the formulation product excellent stability, and the physical and chemical stability of the formulation product can still be maintained after alternating between hot and cold. Moreover, when the liquid medicine is sprayed onto the surface of plant leaves, the evaporation of water in the liquid medicine can be delayed, thereby improving the absorption efficiency of the liquid medicine by the plant leaves.

[0008] Therefore, the first aspect of the present invention provides a liquid crystal oil-in-water pesticide preparation, which comprises, by mass percentage, 1-15% of difenoconazole, 1-25% of pyraclostrobin, 4.8-15% of vegetable oil, 5-20% of polyol, 1-5% of emulsifier, 1-5% of co-emulsifier and 0.1-0.5% of thickener, and the balance is deionized water.

[0009] According to the present invention, the vegetable oil is selected from one or more of palm oil, linseed oil, grapeseed oil and corn germ oil.

[0010] According to the present invention, the polyol is selected from one or more of glycerol, dipropylene glycol, sorbitol and butylene glycol.

[0011] According to the present invention, the emulsifier is a composition of glyceryl monostearate, cetearyl alcohol polyoxyethylene ether and tristyrylphenol polyoxyethylene ether, and the mass ratio of the combination of the three ranges from (1 to 4): (2.1 to 10.2): (2.6 to 5.8).

[0012] According to the present invention, the co-emulsifier is a composition of potassium cetyl phosphate, coconut acid diethanolamide and hydrogenated castor oil, and the mass ratio of the combination of the three is in the range of (0.4-2.8):(1.4-3.3):(2.7-6.3).

[0013] According to the present invention, the thickener is one or more of xanthan gum, carbomer, gum arabic and gelatin.

[0014] The second aspect of the present invention provides a method for preparing the above-mentioned liquid crystal oil-in-water pesticide formulation, comprising the following steps:

[0015] S1: mixing difenoconazole, vegetable oil and emulsifier in proportion, and heating the mixture during stirring until it becomes clear and transparent, to obtain oil phase A;

[0016] S2: stirring and mixing pyraclostrobin, polyol and co-emulsifier according to a certain proportion, and heating the mixture during stirring until it becomes clear and transparent, to obtain oil phase B;

[0017] S3: stirring and mixing the thickener and deionized water in proportion, and obtaining an aqueous phase after the thickener is completely dissolved;

[0018] S4: Slowly add the oil phase A and the oil phase B into the water phase, and stir them homogenously to obtain a liquid crystal oil-in-water pesticide preparation.

[0019] According to the present invention, in the steps S1 and S2, the heating temperature is 80-90°C.

[0020] The third aspect of the present invention provides the use of the above-mentioned liquid crystal oil-in-water pesticide formulation in the prevention and treatment of agricultural pests and diseases.

[0021] According to the present invention, the agricultural pest is grape powdery mildew.

[0022] The beneficial effects of the present invention are as follows:

[0023] 1. The present invention imparts excellent stability to the pesticide formulation product by combining specific emulsifiers and co-emulsifiers, and can maintain the physical and chemical stability of the formulation product after multiple freeze-thaw cycles.

[0024] 2. The present invention prepares a water-in-oil pesticide formulation with a liquid crystal structure by using difenoconazole and pyraclostrobin, which can form a liquid crystal interface between the oil phase and the water phase to form a liquid crystal structure with a stable lattice. When the liquid medicine is sprayed onto the surface of plant leaves, the evaporation of the water content of the liquid medicine can be delayed, and the absorption efficiency of the liquid medicine by the plant leaves can be improved, thereby having an excellent control effect on grape powdery mildew in the field.

[0025] 3. Compared with the prior art, the liquid crystal oil-in-water pesticide formulation of the present invention has a simpler preparation process, does not use a wet grinding process during the production process, greatly reduces energy consumption, and does not use toxic and harmful solvents, which is safe for operators.

[0026] 4. The liquid crystal oil-in-water pesticide preparation of the present invention is prepared using environmentally friendly solvents such as vegetable oil and polyols. The pesticide preparation is in liquid form, which avoids problems such as dust flying during use, is safe for users, and is environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a preparation flow chart of the present invention.

[0028] Figure 2 This is the particle size distribution diagram of the liquid crystal oil-in-water pesticide preparation prepared in Example 3.

[0029] Figure 3 This is the particle size distribution diagram of the liquid crystal oil-in-water pesticide preparation prepared in Comparative Example 1.

[0030] Figure 4This is the particle size distribution diagram of the liquid crystal oil-in-water pesticide preparation prepared in Comparative Example 2. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is clearly and comprehensively described below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to illustrate the content of the present invention, rather than to limit the protection scope of the present invention.

[0032] Unless otherwise specified, the instruments and equipment used in the following examples are conventional devices in the art, and the reagents and samples used are conventional commercially available products.

[0033] Example 1: Preparation of a liquid crystal oil-in-water pesticide formulation (15% difenoconazole, 10% pyraclostrobin)

[0034] According to the components and proportions shown in Table 1, a liquid crystal oil-in-water pesticide formulation with 15% difenoconazole and 10% pyraclostrobin as active ingredients was prepared.

[0035] Table 1

[0036]

[0037] According to the formula in Table 1 above, Figure 1 The process shown in the figure is to prepare a liquid crystal oil-in-water pesticide formulation, and the specific steps are as follows:

[0038] Difenoconazole, linseed oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether, and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase A; pyraclostrobin, butylene glycol, potassium cetyl phosphate, coconut acid diethanolamide, and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase B; gelatin and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B drop to 40°C, the oil phase A and the oil phase B are slowly added to the water phase, and after homogenization and stirring, a liquid crystal oil-in-water pesticide preparation is obtained.

[0039] Example 2: Preparation of a liquid crystal oil-in-water pesticide formulation (10% difenoconazole, 15% pyraclostrobin)

[0040] According to the components and proportions shown in Table 2, a liquid crystal oil-in-water pesticide formulation with 10% difenoconazole and 15% pyraclostrobin as active ingredients was prepared.

[0041] Table 2

[0042]

[0043]

[0044] Difenoconazole, corn germ oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether, and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase A; pyraclostrobin, sorbitol, potassium cetyl phosphate, coconut acid diethanolamide, and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase B; gelatin and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B are reduced to 40°C, the oil phase A and the oil phase B are slowly added into the water phase, and the mixture is homogenized and stirred to obtain a liquid crystal oil-in-water pesticide preparation.

[0045] Example 3: Preparation of a liquid crystal oil-in-water pesticide formulation (15% difenoconazole, 25% pyraclostrobin)

[0046] According to the components and proportions shown in Table 3, a liquid crystal oil-in-water pesticide formulation with 15% difenoconazole and 25% pyraclostrobin as active ingredients was prepared.

[0047] Table 3

[0048]

[0049] Difenoconazole, grape seed oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether, and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase A; pyraclostrobin, dipropylene glycol, potassium cetyl phosphate, coconut acid diethanolamide, and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase B; gum arabic and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B are reduced to 40°C, the oil phase A and the oil phase B are slowly added into the water phase, and after homogenization and stirring, a liquid crystal oil-in-water pesticide preparation is obtained.

[0050] Example 4: Preparation of a liquid crystal oil-in-water pesticide formulation (10% difenoconazole, 10% pyraclostrobin)

[0051] According to the components and proportions shown in Table 4, a liquid crystal oil-in-water pesticide formulation with 10% difenoconazole and 10% pyraclostrobin as active ingredients was prepared.

[0052] Table 4

[0053]

[0054] Difenoconazole, palm oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether, and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase A; pyraclostrobin, glycerol, potassium cetyl phosphate, coconut acid diethanolamide, and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase B; xanthan gum and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B are reduced to 40°C, the oil phase A and the oil phase B are slowly added into the water phase, and after homogenization and stirring, a liquid crystal oil-in-water pesticide preparation is obtained.

[0055] Example 5: Preparation of a liquid crystal oil-in-water pesticide formulation (5% difenoconazole, 5% pyraclostrobin)

[0056] According to the components and proportions shown in Table 5, a liquid crystal oil-in-water pesticide formulation with 5% difenoconazole and 5% pyraclostrobin as active ingredients was prepared.

[0057] Table 5

[0058]

[0059] Difenoconazole, palm oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture is clear and transparent to obtain an oil phase A; pyraclostrobin, glycerol, potassium cetyl phosphate, coconut acid diethanolamide and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture is clear and transparent to obtain an oil phase B; carbomer and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B are reduced to 40°C, the oil phase A and the oil phase B are slowly added into the water phase, and the mixture is homogenized and stirred to obtain a liquid crystal oil-in-water pesticide preparation.

[0060] Example 6: Preparation of a liquid crystal oil-in-water pesticide formulation (15% difenoconazole, 5% pyraclostrobin)

[0061] According to the components and proportions shown in Table 6, a liquid crystal oil-in-water pesticide formulation with 15% difenoconazole and 5% pyraclostrobin as active ingredients was prepared.

[0062] Table 6

[0063]

[0064]

[0065] Difenoconazole, linseed oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether, and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase A; pyraclostrobin, butylene glycol, potassium cetyl phosphate, coconut acid diethanolamide, and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase B; gelatin and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B are reduced to 40°C, the oil phase A and the oil phase B are slowly added into the water phase, and after homogenization and stirring, a liquid crystal oil-in-water pesticide preparation is obtained.

[0066] Example 7: Preparation of a liquid crystal oil-in-water pesticide formulation (5% difenoconazole, 15% pyraclostrobin)

[0067] According to the components and proportions shown in Table 7, a liquid crystal oil-in-water pesticide formulation with 5% difenoconazole and 15% pyraclostrobin as active ingredients was prepared.

[0068] Table 7

[0069]

[0070] Difenoconazole, corn germ oil, glyceryl monostearate, cetearyl alcohol polyoxyethylene ether, and tristyrylphenol polyoxyethylene ether are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase A; pyraclostrobin, sorbitol, potassium cetyl phosphate, coconut acid diethanolamide, and hydrogenated castor oil are stirred and mixed in proportion, and the temperature is increased during the stirring process to 80°C-90°C until the mixture becomes clear and transparent to obtain an oil phase B; gelatin and deionized water are stirred and mixed in proportion to obtain a water phase after they are completely dissolved; when the temperatures of the oil phase A and the oil phase B are reduced to 40°C, the oil phase A and the oil phase B are slowly added into the water phase, and the mixture is homogenized and stirred to obtain a liquid crystal oil-in-water pesticide preparation.

[0071] Comparative Example 1

[0072] The components, proportions and preparation methods of this embodiment are basically the same as those of embodiment 3, except that the proportion of the emulsifier is different.

[0073] The liquid crystal oil-in-water pesticide preparation prepared in this embodiment includes, by mass percentage, 15% of difenoconazole, 25% of pyraclostrobin, 15% of grape seed oil, 5% of dipropylene glycol, 5% of emulsifier monostearate glyceryl: cetearyl alcohol polyoxyethylene ether: tristyrylphenol polyoxyethylene ether (5.8:1.5:6.6): 5%, co-emulsifier potassium cetyl phosphate: coconut acid diethanolamide: hydrogenated castor oil (2.2:3.1:5.8): 5%, 0.1% of gum arabic, and the balance is deionized water.

[0074] Comparative Example 2

[0075] The components, proportions and preparation methods of this embodiment are substantially the same as those of embodiment 3, except that the proportion of the co-emulsifier is different.

[0076] The liquid crystal oil-in-water pesticide preparation prepared in this embodiment includes, by mass percentage, 15% of difenoconazole, 25% of pyraclostrobin, 15% of grape seed oil, 5% of dipropylene glycol, 5% of emulsifier monostearate glyceryl: cetearyl alcohol polyoxyethylene ether: tristyrylphenol polyoxyethylene ether (2.8:6.9:3.6), 5% of auxiliary emulsifier potassium cetyl phosphate: coconut oil diethanolamide: hydrogenated castor oil (3.9:5.6:7.8), 0.1% of gum arabic, and the balance is deionized water.

[0077] Comparative Example 3

[0078] The components, proportions and preparation methods of this embodiment are substantially the same as those of embodiment 3, except that conventional solvents 150# solvent oil and dimethyl sulfoxide are used instead of vegetable oil and polyol.

[0079] The liquid crystal oil-in-water pesticide preparation prepared in this embodiment includes, by mass percentage, 15% of difenoconazole, 25% of pyraclostrobin, 150# solvent oil, 15% of dimethyl sulfoxide, 5% of emulsifier monostearate glyceryl: cetearyl alcohol polyoxyethylene ether: tristyrylphenol polyoxyethylene ether (2.8:6.9:3.6): 5%, co-emulsifier potassium cetyl phosphate: coconut oil diethanolamide: hydrogenated castor oil (2.2:3.1:5.8): 5%, gum arabic: 0.1%, and the balance is deionized water.

[0080] Example 8, Particle Size Distribution Test

[0081] The particle size distribution of the pesticide preparation samples prepared in Example 3, Comparative Example 1 and Comparative Example 2 was tested using a Bettersize 2000 laser particle size analyzer. The test results are as follows: Figure 2-4 shown.

[0082] Depend on Figure 2-4From the comparison results, it can be seen that the particle size of the liquid crystal oil-in-water pesticide preparation prepared in Example 3 is smaller and the particle size distribution is more uniform.

[0083] Example 9, stability test

[0084] Both difenoconazole and pyraclostrobin used in the present invention are low melting point active ingredients. Due to the inherent characteristics of low melting point substances themselves, pesticide formulations with them as active ingredients will be affected by temperature during storage and will experience stratification, flocculation, solidification or crystallization, resulting in a decrease in the suspension rate of the formulation. Therefore, this example investigates the freeze-thaw stability of a liquid crystal oil-in-water pesticide formulation with difenoconazole and pyraclostrobin as active ingredients.

[0085] The liquid crystal oil-in-water pesticide formulations containing difenoconazole and pyraclostrobin as active ingredients prepared in Examples 1 to 7 and Comparative Examples 1 to 3 were subjected to freeze-thaw stability tests. The freeze-thaw stability test was performed in accordance with GB / T 43273-2023. The test results are shown in Table 8 below.

[0086] Table 8 Freeze-thaw stability test results

[0087]

[0088] From the measurement results in Table 8, it can be concluded that the liquid crystal oil-in-water pesticide preparations prepared in Examples 1 to 7 have good freeze-thaw stability, while the pesticide preparations in Comparative Examples 1 and 2 both have problems such as particle size growth, reduced suspension rate, and oil separation after freeze-thaw, and the pesticide preparation in Comparative Example 3 has the problem of crystal precipitation after freeze-thaw.

[0089] Example 10: Evaluation of Moisturizing Performance

[0090] The dynamic film dryness analyzer was used to monitor the microstructural changes of the liquid crystal oil-in-water pesticide formulations containing difenoconazole and pyraclostrobin as active ingredients prepared in Examples 1 to 7 and Comparative Examples 1 to 3 during the curing and drying processes by using multi-speckle-diffuse wave spectroscopy (MS-DWS), and their moisturizing properties were evaluated.

[0091] Take 200 μL of sample and apply it on the substrate to ensure the uniformity and consistency of sample thickness, and place the sample pool under the dynamic dryness analyzer for measurement. Test parameters: 20℃, air humidity 35%, metal substrate. The results of the moisture retention performance test are shown in Table 9 below.

[0092] Table 9 Moisturizing performance test results

[0093]

[0094] From the test results in Table 9, it can be concluded that the liquid crystal oil-in-water pesticide preparations prepared in Examples 1 to 7 have excellent moisturizing properties, and the longest wetting time, touch dry time, and complete drying time can reach 9945 seconds, 15263 seconds, and 17982 seconds, respectively, which are better than the comparative examples. In comparative examples 1 to 3, the longest complete drying time is only 9366 seconds.

[0095] Application Examples

[0096] The test site of this application example is located in Malu Town, Jiading District, Shanghai. Grape powdery mildew occurs in the test site all year round, and the cultivation conditions and water and fertilizer management of each plot are uniform. The field efficacy test method is carried out in accordance with NY / T 1464.12-2007 "Guidelines for Field Efficacy Tests of Pesticides Part 12: Fungicides for Controlling Grape Powdery Mildew".

[0097] The liquid crystal oil-in-water pesticide formulations prepared in Examples 1 to 7 and Comparative Examples 1 to 3 were tested for efficacy against grape powdery mildew in the field, with 40% difenoconazole·pyraclostrobin suspension (commercially available) and 40% difenoconazole·pyraclostrobin microemulsion (commercially available) as control agents. The control effects are shown in Table 10 below:

[0098] Table 10 Field control effect on grape powdery mildew

[0099]

[0100] The test results in Table 10 show that the liquid crystal oil-in-water pesticide preparations prepared in Examples 1 to 7 have excellent control effects on grape powdery mildew in the field, and the control effects 7 days, 14 days, and 28 days after application can reach up to 87.57%, 92.14%, and 90.67%, respectively, which are significantly better than the comparative example and the commercially available control agent, and no pesticide damage is produced, which complies with the green control policy of increasing the efficiency and reducing the dosage of pesticides, and meets the control requirements of agricultural production for grape powdery mildew.

[0101] The above embodiments provide a detailed description of the technical solution of the present application, but are not intended to limit the scope of protection of the present application. Any equivalent substitutions, changes or modifications made according to the spirit of the present application should be included in the scope of protection of the present application.

Claims

1. A liquid crystal oil-in-water pesticide formulation, characterized in that: In terms of mass percentage, the composition comprises 1-15% of difenoconazole, 1-25% of pyraclostrobin, 4.8-15% of vegetable oil, 5-20% of polyol, 1-5% of emulsifier, 1-5% of auxiliary emulsifier and 0.1-0.5% of thickener, and the balance is deionized water.

2. The liquid crystal oil-in-water pesticide formulation according to claim 1, characterized in that: The emulsifier is a composition of glyceryl monostearate, cetearyl alcohol polyoxyethylene ether and tristyrylphenol polyoxyethylene ether, and the mass ratio of the combination of the three is in the range of (1-4):(2.1-10.2):(2.6-5.8).

3. The liquid crystal oil-in-water pesticide formulation according to claim 1, characterized in that: The auxiliary emulsifier is a combination of potassium cetyl phosphate, coconut oil diethanolamide and hydrogenated castor oil, and the mass ratio of the combination of the three is in the range of (0.4-2.8):(1.4-3.3):(2.7-6.3).

4. The liquid crystal oil-in-water pesticide formulation according to claim 1, characterized in that: The vegetable oil is selected from one or more of palm oil, linseed oil, grapeseed oil and corn germ oil.

5. The liquid crystal oil-in-water pesticide formulation according to claim 1, characterized in that: The polyol is selected from one or more of glycerol, dipropylene glycol, sorbitol and butylene glycol.

6. The liquid crystal oil-in-water pesticide formulation according to claim 1, characterized in that: The thickener is one or more of xanthan gum, carbomer, gum arabic and gelatin.

7. The method for preparing the liquid crystal oil-in-water pesticide formulation according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1: mixing difenoconazole, vegetable oil and emulsifier in proportion, and heating the mixture during stirring until it becomes clear and transparent, to obtain an oil phase A; S2: stirring and mixing pyraclostrobin, polyol and co-emulsifier according to a certain proportion, and heating the mixture during stirring until it becomes clear and transparent, to obtain an oil phase B; S3: stirring and mixing the thickener and deionized water in proportion, and obtaining an aqueous phase after the thickener is completely dissolved; S4: Slowly add the oil phase A and the oil phase B into the water phase, and stir them homogenously to obtain a liquid crystal oil-in-water pesticide preparation.

8. The method for preparing the liquid crystal oil-in-water pesticide formulation according to claim 7, characterized in that: In the steps S1 and S2, the heating temperature is 80-90°C.

9. Application of a liquid crystal oil-in-water pesticide formulation, characterized in that: The liquid crystal oil-in-water pesticide preparation is the liquid crystal oil-in-water pesticide preparation according to any one of claims 1 to 6, and is used for preventing and controlling agricultural pests and diseases.

10. The use of the liquid crystal oil-in-water pesticide formulation according to claim 9, characterized in that: The agricultural pest is grape powdery mildew.

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