Comprehensive measurement system for two-photon fluorescent material

Abstract

The invention discloses a comprehensive measurement system for a two-photon fluorescent material. The comprehensive measurement system comprises a laser, a tunable attenuator, a beam splitter, a reference light integrating sphere, a reference light spectrograph, a focus lens, a test light integrating sphere, a test light integrating sphere, a transmission light integrating sphere, a transmission light spectrograph, a stepping motor controller, a computer and the like. By means of the comprehensive measurement system, comprehensive measurement of multiple key performance parameters of the two-photon fluorescent material is achieved, the accuracy and reliability of measurement are improved, and powerful technical support is provided for research and application of the two-photon fluorescent material.

Description

Technical Field

[0001] The invention relates to the field of parameter measurement of two-photon fluorescent materials, in particular to a comprehensive measurement system for two-photon fluorescent materials, which can comprehensively measure the two-photon fluorescence spectrum, nonlinear luminescence quantum efficiency and nonlinear transmittance of the two-photon fluorescent materials. Background Art

[0002] Due to their unique luminescence properties, two-photon fluorescent materials have shown broad application prospects in many fields such as biomedical imaging, optoelectronic devices and information storage. When evaluating the performance of two-photon fluorescent materials, two-photon fluorescence spectrum, quantum efficiency and nonlinear transmittance become three key core parameters. These parameters can not only reflect the optical properties of the material, but also provide key basis for the design and optimization of the material.

[0003] So far, the academic community has made some progress in the performance evaluation methods of two-photon fluorescent materials. For example, the literature [Wang JZ, et al. CdSe / AsS Core-Shell Quantum Dots: Preparation and Two-Photon Fluorescence. J. Am. Chem. Soc. 131 (32): 11300, 2009] proposed an effective measurement method for two-photon fluorescence spectra. This method uses a laser to generate a high-intensity laser beam and focuses it on the sample to be tested through an optical system. Under the action of two-photon absorption, the sample can effectively absorb light energy and release fluorescence signals. These fluorescence signals are collected by the objective lens system and finally captured, recorded and analyzed by the spectrometer, thereby realizing the accurate measurement of the two-photon fluorescence spectrum. Although this method has achieved certain results in measuring two-photon fluorescence spectra, its limitations are also obvious: that is, it is impossible to achieve the simultaneous measurement of the quantum efficiency and nonlinear transmittance of two-photon fluorescent materials, which undoubtedly limits the scope and depth of application of this method in comprehensively evaluating the performance of two-photon fluorescent materials.

[0004] Therefore, developing a new method that can simultaneously measure these three core parameters is of great significance for promoting the research and application of two-photon fluorescent materials. Summary of the invention

[0005] In order to overcome the limitations of the above-mentioned prior art in measuring the performance of two-photon fluorescent materials, especially the inability to accurately measure key parameters such as quantum efficiency and nonlinear luminescence power, the present invention proposes a comprehensive measurement system for two-photon fluorescent materials, which can comprehensively measure the two-photon fluorescence spectrum, nonlinear luminescence quantum efficiency and nonlinear transmittance of two-photon fluorescent materials.

[0006] The technical solution of the present invention is as follows:

[0007] A comprehensive measurement system for two-photon fluorescent materials, characterized in that it includes:

[0008] A laser for generating a laser beam;

[0009] A tunable attenuator, arranged on the path of the laser beam generated by the laser, for adjusting the intensity of the laser beam;

[0010] A beam splitter is arranged on the path of the laser beam adjusted by the tunable attenuator, and is used to split the laser beam into a reference beam and a test beam;

[0011] A reference light integrating sphere, used for collecting the reference light;

[0012] A reference light spectrometer, connected to the reference light integrating sphere via an optical fiber, and used to measure the spectral distribution of the reference light;

[0013] A focusing lens, disposed on the path of the test light, for focusing the test light to a preset focus;

[0014] A test light integrating sphere is arranged at the focus of the focusing mirror, a two-photon fluorescent material sample to be tested is placed inside the sphere, and is used to collect the fluorescence emitted by the sample;

[0015] A test light spectrometer, connected to the test light integrating sphere via an optical fiber, and used for measuring the spectral distribution of the fluorescence;

[0016] A transmitted light integrating sphere, arranged behind the test light integrating sphere, for collecting the test light transmitted from the sample;

[0017] A transmitted light spectrometer, connected to the transmitted light integrating sphere via an optical fiber, and used to measure the spectral distribution of the transmitted light;

[0018] Stepper motor controllers, used to control the movement and position of components in the system;

[0019] The computer is connected to the reference light spectrometer, the test light spectrometer and the transmission light spectrometer, and is used for receiving and processing the spectrum data to calculate the two-photon fluorescence spectrum, the nonlinear luminescence quantum efficiency and the nonlinear transmittance.

[0020] Preferably, the tunable attenuator can continuously adjust the intensity of the laser beam to meet different measurement requirements.

[0021] Preferably, the beam splitter can split the laser beam into two equal beams to ensure that the reference light and the test light have the same initial intensity.

[0022] Preferably, the combination of the test light integrating sphere and the test light spectrometer can measure the fluorescence spectrum emitted by the sample under the two-photon absorption effect, and then calculate the quantum efficiency.

[0023] Preferably, the combination of the tunable attenuator, the reference light integrating sphere, the reference light spectrometer, the transmitted light integrating sphere and the transmitted light spectrometer can measure the nonlinear transmittance of the sample, specifically by comparing the spectral distributions of the reference light and the transmitted light.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] (1) The present invention achieves comprehensive and accurate measurement of the performance of two-photon fluorescent materials by integrating multiple high-precision components, such as tunable attenuators, beam splitters, integrating spheres, and spectrometers, including key parameters such as two-photon fluorescence spectra, nonlinear luminescence quantum efficiency, and nonlinear transmittance.

[0026] (2) The combined use of a high-precision spectrometer and an integrating sphere ensures the comprehensive and accurate collection of fluorescence and transmitted light signals, avoiding inaccurate measurements caused by incomplete signal collection or excessive errors.

[0027] (3) It can measure two-photon fluorescence spectra and absolute luminescence power under different power conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of a traditional system for measuring two-photon fluorescence spectra of two-photon fluorescent materials;

[0029] Figure 2 Schematic diagram of a comprehensive measurement system for two-photon fluorescent materials according to an embodiment of the present invention. DETAILED DESCRIPTION

[0030] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but the protection scope of the present invention shall not be limited thereto.

[0031] A comprehensive measurement system for two-photon fluorescent materials includes a laser, a tunable attenuator, a beam splitter, a reference light integrating sphere, a reference light spectrometer, a focusing mirror, a test light integrating sphere, a test light integrating sphere, a transmitted light integrating sphere, a transmitted light spectrometer, a stepper motor controller, and a computer. The tunable attenuator and the beam splitter are placed in sequence along the light beam emission direction of the laser. The beam splitter divides the incident light beam into a reflected light beam and a transmitted light beam. The reflected light beam is used as a reference light beam. In the propagation direction of the reference light beam is the reference light integrating sphere. The reference light spectrometer is connected to the port of the reference light integrating sphere through an optical fiber. The transmitted light beam is a test light beam. In the propagation direction of the test light beam are the focusing mirror, the test light integrating sphere, and the transmitted light integrating sphere. The test light spectrometer is connected to the port of the test light integrating sphere through an optical fiber. The transmitted light spectrometer is connected to the port of the transmitted light integrating sphere through an optical fiber. The stepper motor controller is connected to the tunable attenuator. The stepping motor controller, the reference light spectrometer, the test light spectrometer and the transmission light spectrometer are connected to the computer.

[0032] The tunable attenuator, test light integrating sphere and test light spectrometer are used to measure the fluorescence spectrum and quantum efficiency of the sample, and the reference light integrating sphere, reference light spectrometer, transmitted light integrating sphere and transmitted light spectrometer are used to measure the nonlinear transmittance of the sample.

[0033] Working principle details:

[0034] Laser light source and beam control: The laser beam generated by the laser first passes through a tunable attenuator, which can accurately adjust the laser intensity to meet different measurement requirements. Subsequently, the laser beam is split into two beams by a beam splitter: one as a reference light and the other as a test light.

[0035] Reference light path: The reference light is guided directly into the reference light integrating sphere, which is responsible for collecting and homogenizing the light intensity. The reference light spectrometer is connected to the reference light integrating sphere via an optical fiber to measure the spectral distribution of the reference light and provide a benchmark for subsequent calculations.

[0036] Test light path and sample measurement: After being precisely focused by the focusing mirror, the test light is irradiated onto the sample located at the center of the test light integrating sphere. The sample emits fluorescence under the two-photon absorption effect, and these fluorescence signals are collected by the test light integrating sphere and transmitted to the test light spectrometer through optical fiber for spectral analysis.

[0037] Transmitted light measurement: At the same time, the light transmitted from the sample is collected by the transmitted light integrating sphere. The transmitted light spectrometer is connected to the transmitted light integrating sphere through an optical fiber to measure the spectral distribution of the transmitted light, which is crucial for calculating the nonlinear transmittance.

[0038] System control and data processing: The stepper motor controller is responsible for precisely controlling the movement and position of each component to ensure the accuracy and repeatability of the measurement. As the control center of the system, the computer is responsible for receiving data from each spectrometer and performing complex calculations and analyses to obtain key parameters such as two-photon fluorescence spectrum, nonlinear luminescence quantum efficiency, and nonlinear transmittance.

[0039] The comprehensive measurement system of the present invention not only realizes the comprehensive measurement of multiple key performance parameters of two-photon fluorescent materials, but also improves the accuracy and reliability of the measurement, providing strong technical support for the research and application of two-photon fluorescent materials.

[0040] Example:

[0041] The laser is a femtosecond laser with a central wavelength of 800nm. The transmittance range of the tunable attenuator is 3%-100%. The beam splitter is fused quartz glass with a reflectivity of about 10% on the front and back surfaces and a transmittance of 90%. The inner diameter of the reference light integrating sphere is 150mm, the reflectivity of the internal coating is 97%, and the number of openings is 3. The spectral detection range of the reference light spectrometer is 350-950nm, and the slit size is 70μm. The focal length of the focusing mirror is 150mm. The inner diameter of the test light integrating sphere is 50mm, the reflectivity of the internal coating is 97%, and the number of openings is 4. The spectral detection range of the test light spectrometer is 340-1015nm. The inner diameter of the transmitted light integrating sphere is 150mm, the reflectivity of the internal coating is 97%, and the number of openings is 4. The spectral detection range of the transmitted light spectrometer is 350-950nm, and the slit size is 70μm.

[0042] The comprehensive measurement system of the present invention not only realizes the comprehensive measurement of multiple key performance parameters of two-photon fluorescent materials, but also improves the accuracy and reliability of the measurement, providing strong technical support for the research and application of two-photon fluorescent materials.

Claims

1. A comprehensive measurement system for two-photon fluorescent materials, characterized in that: include: A laser for generating a laser beam; A tunable attenuator, arranged on the path of the laser beam generated by the laser, for adjusting the intensity of the laser beam; A beam splitter is arranged on the path of the laser beam adjusted by the tunable attenuator, and is used to split the laser beam into a reference beam and a test beam; A reference light integrating sphere, used for collecting the reference light; A reference light spectrometer, connected to the reference light integrating sphere via an optical fiber, and used to measure the spectral distribution of the reference light; A focusing lens, disposed on the path of the test light, for focusing the test light to a preset focus; A test light integrating sphere is arranged at the focus of the focusing mirror, a two-photon fluorescent material sample to be tested is placed inside the sphere, and is used to collect the fluorescence emitted by the sample; A test light spectrometer, connected to the test light integrating sphere via an optical fiber, and used for measuring the spectral distribution of the fluorescence; A transmitted light integrating sphere, arranged behind the test light integrating sphere, for collecting the test light transmitted from the sample; A transmitted light spectrometer, connected to the transmitted light integrating sphere via an optical fiber, and used to measure the spectral distribution of the transmitted light; Stepper motor controllers, used to control the movement and position of components in the system; The computer is connected to the reference light spectrometer, the test light spectrometer and the transmission light spectrometer, and is used for receiving and processing the spectrum data to calculate the two-photon fluorescence spectrum, the nonlinear luminescence quantum efficiency and the nonlinear transmittance.

2. The comprehensive measurement system of two-photon fluorescent materials according to claim 1, characterized in that: The tunable attenuator can continuously adjust the intensity of the laser beam to meet different measurement requirements.

3. The comprehensive measurement system of two-photon fluorescent materials according to claim 1, characterized in that: The beam splitter can equally split the laser beam into two beams to ensure that the reference light and the test light have the same initial intensity.

4. The comprehensive measurement system of two-photon fluorescent materials according to claim 1, characterized in that: The combination of the test light integrating sphere and the test light spectrometer can measure the fluorescence spectrum emitted by the sample under the two-photon absorption effect, and then calculate the quantum efficiency.

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