Method of cell culture and cell product

Abstract

The invention relates to the technical field of cell culture, and discloses a cell culture method and a cell product.The method comprises the steps that polyester fiber cloth is sterilized and then placed in a culture solution, and then cells are inoculated for in-vitro culture; wherein the polyester fiber cloth is formed by self-adhesion of polyester fibers with skin-core structures; the thickness of the polyester fiber cloth is 0.4-0.5 mm, and the surface density of the polyester fiber cloth is 80-130 g / m < 2 >. By adopting the cell culture method disclosed by the invention, the glycometabolism capability of the cells can be obviously improved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a cell culture method and a cell product. Background Art

[0002] At present, large-scale animal cell culture technology has been widely used in the production of various types of cells and cell products, including enzymes, growth factors, vaccines and antibodies and other biological products. Most animal cells have the habit of adhering to the wall for growth, so when in vitro culture is carried out, it is usually necessary to provide a support for its growth. This support refers to the cell culture carrier commonly referred to by those skilled in the art. The common cell culture carriers in China at present are granular carriers with a diameter of 2-11mm, porous spherical carriers or sheet-like fiber carriers. Current microcarriers include silk fibroin and chitosan macroporous microcarriers, chitosan and gelatin mixed microcarriers, etc. However, spherical microcarriers have the problem that cells can only attach to the surface and the area-to-volume ratio is small, and although porous microcarriers have gaps to facilitate cell attachment, their gaps are easily blocked and then affect oxygen and material exchange. At present, research on fiber scaffold carriers at home and abroad is relatively rare, and the main fiber scaffold is an electrostatic spinning material, which is mainly used in tissue engineering in vivo transplantation, while it is less for purely large-scale cell culture in vitro. The structural characteristics of electrospun fiber scaffolds are that the electrospun fibers are nano-scale fine structures with many gaps, which can simply imitate the distribution of extracellular matrix fibers for cell attachment and growth. However, the gaps are small, and cells can easily block the related gaps after attaching in sheets, which severely limits material exchange. In addition, they easily float on the surface of the culture medium, which is not conducive to the contact between cells and the culture medium. Summary of the invention

[0003] The purpose of the present invention is to overcome the problem of low cell survival rate of the existing fiber cloth in the prior art when used for cell culture, and to provide a cell culture method and cell product.

[0004] In order to achieve the above-mentioned object, the first aspect of the present invention provides a method for cell culture, which comprises: sterilizing a polyester fiber cloth and placing it in a culture medium, and then inoculating cells for in vitro culture; wherein the polyester fiber cloth is self-adhesive with polyester fibers having a skin-core structure; the thickness of the polyester fiber cloth is 0.4-0.5 mm, and the surface density is 80-130 g / m 2 .

[0005] The second aspect of the present invention provides a cell product prepared by the method described above.

[0006] The cell culture method of the present invention can significantly improve the sugar metabolism ability of cells. The higher the sugar metabolism ability, the higher the cell reproduction rate and survival rate. Therefore, the method of the present invention can significantly improve the survival rate of cells and the cell reproduction rate is faster. DETAILED DESCRIPTION

[0007] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0008] The first aspect of the present invention provides a method for cell culture, comprising: sterilizing a polyester fiber cloth and placing it in a culture medium, and then inoculating cells for in vitro culture; wherein the polyester fiber cloth is self-adhesive with polyester fibers having a skin-core structure; the thickness of the polyester fiber cloth is 0.4-0.5 mm, and the surface density is 80-130 g / m 2 .

[0009] In the present invention, the thickness of the polyester fiber can be 0.4 mm, 0.41 mm, 0.42 mm, 0.43 mm, 0.44 mm, 0.45 mm, 0.46 mm, 0.47 mm, 0.48 mm, 0.49 mm, 0.5 mm, and a range consisting of any two of the above points. The surface density of the polyester fiber can be 80 g / m 2 , 85g / m 2 , 90g / m 2 , 95g / m 2 , 100g / m 2 , 105g / m 2 , 110g / m 2 , 115g / m 2 , 120g / m 2 , 125g / m 2 , 130g / m 2 , and the range consisting of any two of the above points.

[0010] According to the present invention, the melting point of the core layer material of the polyester fiber is more than 20°C higher than the melting point of the skin layer material (for example, 20°C, 22°C, 24°C, 25°C, 30°C, 40°C, 50°C, 60°C, 80°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, and a range formed by any two of the above points, for example, 20-160°C). Preferably, the melting point of the core layer material differs from that of the skin layer material by more than 24°C.

[0011] According to the present invention, preferably, the mass ratio of the core layer material to the skin layer material of the polyester fiber is x, and 1.5≤x≤4.

[0012] According to the present invention, the inventors have further discovered that when a fiber cloth prepared from polyester fibers whose mass ratio x of the core layer material to the skin layer material and the average diameter D of the polyester fibers satisfy the relationship of formula (1) is used for cell culture, the sugar metabolism capacity of the cells can be further improved.

[0013]

[0014] More preferably, when the mass ratio of the core layer material to the skin layer material of the polyester fiber is 2-3 and satisfies the formula (1), the sugar metabolism capacity of cells can be further improved when the composite polyester fiber is used for cell culture.

[0015] According to the present invention, preferably, the intrinsic viscosity of the sheath material of the polyester fiber is 0.01-0.18 dL / g higher than that of the core material, and the standard deviation of the intrinsic viscosities of the core material and the sheath material is ≤0.015 dL / g.

[0016] In the present invention, the test method for the intrinsic viscosity of polyester is the intrinsic viscosity test method described in GBT14190-2017. The test method for the standard deviation of the intrinsic viscosity is: randomly take 6 samples, test the intrinsic viscosity according to the intrinsic viscosity test method described in GBT14190-2017, and take the standard deviation of the values. The smaller the standard deviation of the intrinsic viscosity, the higher the uniformity of the intrinsic viscosity of the same batch of materials.

[0017] According to the present invention, the standard deviation of the intrinsic viscosity of the core layer material and the skin layer material of the polyester fiber can be independently 0.018 dL / g, 0.015 dL / g, 0.01 dL / g, 0.009 dL / g, 0.008 dL / g, 0.007 dL / g, 0.006 dL / g, 0.005 dL / g, 0.004 dL / g, 0.003 dL / g, 0.002 dL / g, 0.001 dL / g, 0.0001 dL / g, and a range consisting of any two of the above points, for example, 0.0001-0.018 dL / g.

[0018] According to the present invention, preferably, the core layer material of the polyester fiber is homopolyethylene terephthalate.

[0019] According to the present invention, preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.62-0.8 dL / g (for example, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.8 dL / g, and a range consisting of any two of the above points), more preferably 0.65-0.7 dL / g, and the standard deviation of the intrinsic viscosity is ≤0.01 dL / g.

[0020] According to the present invention, preferably, the melting point of the homopolyethylene terephthalate is above 240°C (for example, 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and a range consisting of any two of the above points), more preferably 245-265°C.

[0021] According to the present invention, preferably, the skin material of the polyester fiber is co-polyethylene terephthalate.

[0022] According to the present invention, preferably, the intrinsic viscosity of the copolyethylene terephthalate is 0.52-0.9 dL / g (for example, 0.5 dL / g, 0.52 dL / g, 0.54 dL / g, 0.60 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.86 dL / g, 0.88 dL / g, 0.9 dL / g, and a range formed by any two of the above points), more preferably 0.6-0.8 dL / g, and the standard deviation of the intrinsic viscosity is ≤0.01 dL / g.

[0023] According to the present invention, preferably, the melting point of the copolyethylene terephthalate is below 240°C (for example, 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and a range formed by any two of the above points), more preferably 130-240°C.

[0024] According to the present invention, preferably, the copolyethylene terephthalate comprises structural units from terephthalic acid, ethylene glycol and comonomer Y, and the content of the structural units from comonomer Y in the copolyethylene terephthalate is 0.6-20 mol% (for example, 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, and the range composed of any two of the above points), more preferably 0.7-18 mol%. In the present invention, the content of the structural unit of comonomer Y refers to: the amount of the structural unit of comonomer Y accounts for the percentage of the total amount of the structural units of terephthalic acid, ethylene glycol and comonomer Y.

[0025] According to the present invention, the type of the comonomer is not particularly limited, as long as the melting point and intrinsic viscosity difference between the skin material and the core material and the standard deviation of the intrinsic viscosity can meet the requirements. Preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and is more preferably isophthalic acid and / or phthalic acid. More preferably, the diol is a diol having a carbon number of 3-20, and is more preferably at least one of butanediol, hexanediol, cyclohexanedimethanol and pentanediol (e.g., neopentanediol). More preferably, the tetraol has a carbon number of 3-20. In the present invention, the carbon number of the diol or tetraol can be 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20 independently of each other.

[0026] In the present invention, the comonomer Y may also be selected from acid monomers in other forms such as anhydrides, acid chlorides, methanol esters, and ethanol esters, and the alcohol monomers include alcohol monomers in other forms such as ethers, hemiacetals, and acetals.

[0027] According to the present invention, preferably, the Sb content in the core layer material and the skin layer material of the polyester fiber is less than 20 ppm. In the present invention, "ppm" refers to the "weight" content.

[0028] According to the present invention, preferably, the average diameter of the polyester fiber is 18-30 μm, and the diameter standard deviation is ≤2.2 μm. Preferably, the fiber cloth prepared by the polyester fiber meeting the above average diameter and diameter standard deviation can further improve the cell reproduction rate and survival rate, so that the cell sugar metabolism value is above 15 g / day; and when the average diameter and diameter standard deviation of the polyester fiber deviate from the above range, the cell sugar metabolism value is below 10 g / day, see the patent application "Composite polyester fiber and its preparation method and application" submitted by the inventor on the same day.

[0029] In the present invention, the average diameter of the polyester fiber can be 18 μm, 20 μm, 22 μm, 24 μm, 26 μm, 28 μm, 30 μm, and a range formed by any two of the above points. The standard deviation of the diameter of the polyester fiber can be 2.2 μm, 2 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, and a range formed by any two of the above points, for example, 0.1-2.2 μm.

[0030] In the present invention, the test method of the diameter standard deviation is: randomly select 50 polyester fibers, randomly cut a small section from each fiber, take a scanning electron microscope (SEM) photo, and measure the diameter of each fiber from the photo, accurate to 0.1 μm, and then calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0031] In the present invention, polyester fiber refers to polyester fiber having a length greater than 150 mm.

[0032] According to the present invention, preferably, the polyester fiber is self-bonded by laying and hot pressing the polyester fiber with a core-skin structure to obtain a polyester fiber cloth. More preferably, the hot pressing temperature is 180-220°C.

[0033] According to the present invention, preferably, the sterilization method includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization.

[0034] According to the present invention, preferably, the conditions for high-temperature steam sterilization include: a temperature of 115-135°C (for example, 115°C, 120°C, 125°C, 130°C, 135°C, and a range consisting of any two of the above), and a time of 10-60min (for example, 10min, 20min, 30min, 40min, 50min, 60min, and a range consisting of any two of the above).

[0035] According to the present invention, preferably, the conditions for irradiation sterilization include: irradiating the pretreated fiber cloth with high-energy electron rays, with an irradiation dose of 5-30 kGy and an irradiation time of less than 10 s.

[0036] According to the present invention, preferably, the method further comprises: pre-treating the fiber cloth before the sterilization treatment, wherein the pre-treatment is performed on the fiber cloth using a hydrogen peroxide solution.

[0037] According to the present invention, preferably, the concentration of H2O2 in the hydrogen peroxide solution is 5-10% by weight.

[0038] According to the present invention, preferably, the pretreatment conditions include: temperature of 15-40° C., and time of 0.5-4 h.

[0039] According to the present invention, preferably, the method further comprises: cleaning the pretreated fiber cloth under ultrasound, and then sterilizing the pretreated fiber cloth.

[0040] According to the present invention, preferably, the cleaning agent used for cleaning is water, preferably double distilled water and / or ultrapure water.

[0041] According to the present invention, preferably, the cells are adherent cells, preferably Vero cells and / or L929 cells.

[0042] The present invention also provides a method for preparing polyester fiber, the method comprising: melting a core layer material and a skin layer material respectively to obtain a melt, and then spinning the melt to obtain a polyester fiber with a skin-core structure;

[0043] The melting point of the core material is higher than that of the skin material by more than 20°C; the intrinsic viscosity of the skin material is 0.01-0.18 dL / g higher than that of the core material, and the standard deviation of the intrinsic viscosities of the core material and the skin material is ≤0.015 dL / g; the mass ratio of the core material to the skin material is x, 1.5≤x≤4;

[0044] The spinneret holes of the spinneret used in the spinning process are in the shape of concentric circles, the difference between the maximum diameter and the minimum diameter of each inner circle of the spinneret holes is ≤2 μm, and the difference between the maximum diameter and the minimum diameter of each outer circle is ≤2 μm. The difference between the maximum diameter and the minimum diameter of each inner circle or each outer circle can be independently 0.1 μm, 0.5 μm, 1.5 μm, 2 μm, and a range consisting of any two of the above points, such as 0.1-2 μm.

[0045] In the present invention, the amount of the core layer material is higher than that of the skin layer material, and the mass ratio (weight ratio) of the core layer material to the skin layer material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.2, 3.5, 3.8, 4, and a range consisting of any two of the above points, preferably 2-3.6. Limiting the weight ratio of the core layer material to the skin layer material within the above preferred range can further improve the uniformity of the thickness of the polyester fiber, that is, obtain a smaller standard deviation of the polyester fiber diameter.

[0046] In the present invention, it can be understood that the core layer material forms the core layer structure of the polyester fiber after passing through the inner circle of the spinneret hole, and the skin layer material forms the skin layer structure of the polyester fiber after passing through the outer circle of the spinneret hole, and then after cooling and stretching, a polyester fiber with a skin-core structure is formed.

[0047] According to the present invention, preferably, the inner diameter of the inner circle of the spinneret hole is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm. In the present invention, the precise hole diameter of the spinneret is measured by a profilometer.

[0048] According to the present invention, preferably, the core layer material melt is obtained by melting the core layer material at 220-280° C. (eg, 220° C., 240° C., 260° C., 280° C., and a range consisting of any two of the above).

[0049] According to the present invention, preferably, the skin material melt is obtained by melting the skin material at 200-280°C (eg, 200°C, 220°C, 240°C, 260°C, 280°C, and a range consisting of any two of the above).

[0050] In the present invention, it is understood that the temperature of the core layer material melt and the skin layer material melt refers to the temperature of the spinneret when the core layer material melt and the skin layer material melt are extruded from the spinneret outlet.

[0051] According to the present invention, preferably, the process of spinning the melt to obtain a polyester fiber having a sheath-core structure comprises the following steps:

[0052] (1) feeding the melt into a spinneret for spinning to obtain a filamentary melt having a skin-core structure;

[0053] (2) using side blowing to cool a filamentary melt having a skin-core structure to obtain a nascent fiber;

[0054] (3) The nascent fibers are stretched in a circular tube using a high-speed airflow to obtain polyester fibers.

[0055] According to the present invention, preferably, the wind speed of the side blowing is 1.5-1.8 m / s (for example, 1.5 m / s, 1.6 m / s, 1.7 m / s, 1.8 m / s, and a range formed by any two of the above points), and the temperature is 15-20°C.

[0056] According to the present invention, preferably, the flow rate of the high-speed airflow is 3000-5000 m / min. Preferably, the high-speed airflow stretching can further preferentially orient the fiber structure along the processing direction of the fiber, thereby making the fiber thickness more uniform on the one hand and making the mechanical properties of the fiber better on the other hand.

[0057] According to the present invention, preferably, the stretching method comprises at least one of a round tube stretching method, a slit stretching method and a swing wire method.

[0058] According to the present invention, preferably, the inner diameter of the round tube used in the round tube method is 0.5-5 cm, and the distance between two adjacent round tubes is 0.5-10 cm. The distance between two adjacent round tubes refers to the distance between the centers of the two adjacent round tubes. The length of the round tube can be 0.1-2 m. The length of the round tube refers to the distance along the direction of fiber ejection.

[0059] The second aspect of the present invention provides a cell product prepared by the method described above.

[0060] The present invention will be described in detail below by way of examples. In the following examples,

[0061] Unless otherwise specified, the spinneret is manufactured by Changzhou Jier Precision Machinery Manufacturing Co., Ltd. and is customized as required.

[0062] The Sb content in the core layer material and the skin layer material is less than 20 ppm.

[0063] The room temperature is about "15-20℃".

[0064] In the following embodiments, the homemade polyester involved is prepared by mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and an optional comonomer Y (e.g., cyclohexanedimethanol, analytical grade) in a reactor according to the required amount of the product, heating to 258-263° C., reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290° C., and evacuating with an oil pump, reacting for about 2 hours to obtain the desired PET.

[0065] The test method for the average diameter of polyester fibers is as follows: randomly select 50 polyester fibers, cut a small section at random, take scanning electron microscope (SEM) photos, and measure the diameter of each fiber from the photos, accurate to 0.1μm, and then calculate the average value of these diameter values ​​according to the average value calculation method; and calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0066] Example 1

[0067] Homopolyethylene terephthalate (manufacturer: Sinopec Yizheng Chemical Fiber Co., Ltd., hereinafter referred to as "Yizheng Chemical Fiber", model FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261°C) was used as the core material. Copolyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, comonomer cyclohexanedimethanol, comonomer content 15 mol%, melting point 140°C) was used as the skin material. The mass ratio of the core material to the skin material was 3, and the core material was extruded from a spinneret at an outlet temperature of about 280°C. Among them, there are 48 small holes with skin-core concentric structure distributed on the spinneret, the inner diameter of the inner hole is 20 microns, the range (the diameter of the largest hole minus the diameter of the smallest hole) is 1.2 microns, and the inner diameter of the outer hole is 30 microns, and the range is 1.1 microns. After the polymer melt is extruded from the spinneret, it is blown and cooled with room temperature wind of 1.5-1.8m / s, and the cooled fibers are sent into 5 steel round tubes (the inner diameter of the round tube is 2cm, the distance between two adjacent round tubes is 3.5cm, and the length of the round tube is 1m) on average or nearly average, and the cooled filaments are stretched in the round tube at a wind speed of about 4000-4500m / min to obtain polyester fibers. The average diameter of the polyester fiber is tested and the diameter standard deviation is calculated. The test results are shown in Table 1.

[0068] The polyester fiber is laid on a web laying machine, and then the fiber cloth of a certain thickness is hot pressed at 190-200℃ using a non-embossed polished hot roller to obtain a fiber cloth of a specific thickness. The test method for the surface density of the fiber cloth is: use a 1 / 10,000 balance to measure the surface density of 1cm 2 The fiber cloth of the area is weighed, and then the mass of the fiber cloth is divided by its area; the test method of the fiber cloth thickness is: using a dial thickness gauge (model 547-313) from Suzhou Quantum Instrument Co., Ltd. The surface density and thickness of the fiber cloth are shown in Table 1.

[0069] Example 2

[0070] The method of Example 1 was followed, except that copolyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol%, melting point 237°C) was used as the skin material.

[0071] Example 3

[0072] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 2, the inner diameter of the inner hole of the spinneret used is 18 microns, the range is 1.1 microns, and the inner diameter of the outer hole is 32 microns, the range is 1.6 microns.

[0073] Example 4

[0074] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 3.6.

[0075] Example 5

[0076] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.6.

[0077] Comparative Example 1-2

[0078] The method of Example 1 was followed, except that different surface densities and thicknesses of the fiber cloth were obtained by adjusting the process parameters of the web laying and hot pressing. The surface density and thickness of the fiber cloth are shown in Table 1.

[0079] Test Example 1

[0080] Pretreatment and sterilization of fiber cloth: Soak the fiber cloth in a 7 wt% hydrogen peroxide aqueous solution at room temperature for 60 minutes; then use ultrapure water to ultrasonically clean the fiber cloth, and then dry it at 80°C. After drying, sterilize the fiber cloth with high-temperature steam at a temperature of 121°C for 60 minutes.

[0081] Then the sugar metabolism ability of Vero cells was tested when the sterilized fiber cloth was used as a carrier. The test method for sugar metabolism is: cut the fiber cloth into small pieces of 6mm×6mm fiber cloth, place 100g of 6mm×6mm fiber cloth, 8L PBS buffer and about 200mL of 199 culture medium (purchased from Beijing Tsinghua Tianyi Biotechnology Co., Ltd.) in a 10L basket reactor, add 10% high-quality newborn calf serum (purchased from Lanzhou Minhai Biotechnology Co., Ltd.) relative to the culture medium, and add an appropriate amount of 20% by weight concentration of glucose so that the total glucose concentration is 0.5% by weight. Inoculate Vero cells (purchased from the National Biomedical Experimental Cell Resource Bank, 4th generation Vero cells, every 1cm 2 The inoculation amount of cells on the fiber cloth was 0.5×10 6), aeration culture at 37°C, pH 7.3, DO (percentage of dissolved oxygen in saturated dissolved oxygen) 60%, 5% carbon dioxide concentration, and slow stirring during culture for 7 days. Samples were taken every 24 hours and the glucose content was detected using a glucose detection kit (purchased from Nanjing Jiancheng Bioengineering Institute), and the glucose concentration was supplemented to 0.5% by weight with a 20% by weight glucose solution. The measured reduction in glucose content was converted into a glucose consumption rate value in g / day, and a curve of glucose consumption rate versus culture time was drawn, and then the value at 100h was taken as the 100h sugar metabolism rate value. The 100h sugar metabolism rate value is shown in Table 1.

[0082] Table 1

[0083]

[0084] It can be seen from the results in Table 1 that the diameter standard deviation of the polyester fiber prepared by the present invention is small, so the polyester fiber prepared by the present invention has better uniformity. When the fiber cloth prepared from the polyester fiber is used for cell culture, it can significantly improve the sugar metabolism ability of cells.

[0085] The preferred embodiments of the present invention are described in detail above, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various technical features in any other suitable manner, and these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A method for cell culture, characterized in that: The method comprises: sterilizing a polyester fiber cloth and placing it in a culture medium, then inoculating cells for in vitro culture; wherein the polyester fiber cloth is self-adhesive with polyester fibers having a skin-core structure; the thickness of the polyester fiber cloth is 0.4-0.5 mm, and the surface density is 80-130 g / m 2 .

2. The method according to claim 1, wherein The core material of the polyester fiber is homopolyethylene terephthalate; Preferably, the homopolyethylene terephthalate has an intrinsic viscosity of 0.62-0.8 dL / g, a standard deviation of the intrinsic viscosity of ≤0.01 dL / g, and a melting point of 245-265°C.

3. The method according to claim 1, wherein The cortex material of the polyester fiber is copolyethylene terephthalate; Preferably, the intrinsic viscosity of the co-polyethylene terephthalate is 0.52-0.9 dL / g, the standard deviation of the intrinsic viscosity is ≤0.01 dL / g, and the melting point is 130-240°C.

4. The method according to claim 3, wherein: The copolyethylene terephthalate comprises structural units derived from terephthalic acid, ethylene glycol and comonomer Y, and the content of the structural units derived from comonomer Y in the copolyethylene terephthalate is 0.6-20 mol %, more preferably 0.7-18 mol %; Preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols and tetraols.

5. The method according to claim 1, wherein: The sterilization method includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization.

6. The method according to claim 5, wherein: The conditions for high temperature steam sterilization include: temperature of 115-135° C. and time of 10-60 min.

7. The method according to claim 1, wherein: The method further comprises: before the sterilization treatment, pre-treating the polyester fiber cloth, wherein the pre-treating method is to pre-treat the polyester fiber cloth with a hydrogen peroxide solution.

8. The method according to claim 1, wherein: The pretreatment conditions include: temperature of 15-40°C and time of 0.5-4h.

9. The method according to claim 1, wherein: The cells are adherent cells, preferably Vero cells and / or L929 cells.

10. A cell product prepared by the method according to any one of claims 1 to 9.

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