Method for preparing polyester fiber cloth through slit method, polyester fiber cloth and application of polyester fiber cloth

Abstract

The invention relates to the technical field of polyester fiber cloth, and discloses a method for preparing polyester fiber cloth through a slit method, the polyester fiber cloth and application of the polyester fiber cloth, and the method comprises the following steps: (1) feeding a molten core layer material and a skin layer material into a spinneret plate for spinning to obtain a filamentous melt with a skin-core structure; (2) cooling the filiform melt with the skin-core structure to obtain nascent fibers; (3) stretching the nascent fiber in the slit to obtain the polyester fiber with a skin-core structure; and (4) taking the polyester fibers with the average diameter of 18-30 microns and the diameter standard deviation of less than or equal to 2.2 microns, and carrying out lapping and hot pressing to obtain the polyester fiber cloth. When the fiber cloth prepared by the method is used for cell culture, the glycometabolism capability of cells can be obviously improved, and the cell survival rate is improved.

Description

Technical Field

[0001] The invention relates to the technical field of polyester fiber cloth, and in particular to a method for preparing polyester fiber cloth by a slit method, the polyester fiber cloth and applications thereof. Background Art

[0002] At present, large-scale animal cell culture technology has been widely used in the production of various types of cells and cell products, including enzymes, growth factors, vaccines and antibodies and other biological products. Most animal cells have the habit of growing on the wall, so when they are cultured in vitro, it is usually necessary to provide a support for their growth. This support refers to the cell culture carrier commonly referred to by those skilled in the art. At present, the common cell culture carriers in China are granular carriers (diameter 2-11mm), porous microsphere carriers (diameter <1mm) or sheet fiber cloth carriers. However, there are problems that cells can only attach to the surface and the area-to-volume ratio of granular carriers is small, and although there are gaps in porous microsphere carriers, which are conducive to cell attachment, their gaps are easily blocked and thus affect oxygen and material exchange. Sheet fiber cloth carriers can overcome the above determination and have high culture efficiency, but there are relatively few related studies at home and abroad. The existing electrostatic spinning scheme is mainly used, which is mainly used in tissue engineering in vivo transplantation, and is rarely used for large-scale cell culture in vitro, because its overall culture effect is not good enough. Summary of the invention

[0003] The purpose of the present invention is to overcome the above-mentioned problems in the prior art and to provide a method for preparing polyester fiber cloth by a slit method, polyester fiber cloth and applications thereof.

[0004] In order to achieve the above object, the first aspect of the present invention provides a method for preparing a polyester fiber cloth, the method comprising:

[0005] (1) feeding the melted core layer material and the skin layer material into a spinneret for spinning to obtain a filamentary melt having a skin-core structure;

[0006] (2) cooling the filament melt having a skin-core structure to obtain a primary fiber;

[0007] (3) stretching the spun fiber in a slit to obtain a polyester fiber with a skin-core structure;

[0008] (4) Polyester fibers with an average diameter of 17-32 μm and a diameter standard deviation of ≤2.2 μm are laid and hot-pressed to obtain polyester fiber cloth.

[0009] The second aspect of the present invention provides a polyester fiber cloth prepared by the method described above.

[0010] The third aspect of the present invention provides a polyester fiber cloth, which is made of polyester fibers with a skin-core structure and self-bonded, and the thickness of the polyester fiber cloth is 0.4-0.5mm, and the surface density is 80-130g / m 2 ; Among them, the average diameter of polyester fiber is 17-32μm, and the standard deviation of diameter is ≤2.2μm.

[0011] The fourth aspect of the present invention provides use of the above-mentioned polyester fiber cloth in cell culture.

[0012] When the fiber cloth prepared by the method of the present invention is used for cell culture, it can significantly improve the sugar metabolism ability of cells. The higher the sugar metabolism ability, the higher the cell reproduction rate and survival rate (only living cells can metabolize sugar, and dead cells cannot metabolize sugar). That is, the composite polyester fiber can achieve better results when used for cell culture. DETAILED DESCRIPTION

[0013] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0014] A first aspect of the present invention provides a method for preparing a polyester fiber cloth, the method comprising:

[0015] (1) feeding the melted core layer material and the skin layer material into a spinneret for spinning to obtain a filamentary melt having a skin-core structure;

[0016] (2) cooling the filament melt having a skin-core structure to obtain a primary fiber;

[0017] (3) stretching the spun fiber in a slit to obtain a polyester fiber with a skin-core structure;

[0018] (4) Polyester fibers with an average diameter of 17-32 μm and a diameter standard deviation of ≤2.2 μm are laid and hot-pressed to obtain polyester fiber cloth.

[0019] According to the present invention, preferably, the average diameter of the polyester fiber is 17-32 μm, and the standard deviation of the diameter is ≤ 2.2 μm. In the present invention, the average diameter of the polyester fiber can be 17 μm, 18 μm, 20 μm, 22 μm, 24 μm, 26 μm, 28 μm, 30 μm, 32 μm, and a range consisting of any two of the above points. The standard deviation of the diameter of the polyester fiber can be 2.2 μm, 2 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, and a range consisting of any two of the above points, for example, 0.1-2.2 μm.

[0020] In the present invention, the test method of the diameter standard deviation is: randomly select 50 polyester fibers, randomly cut a small section from each fiber, take a scanning electron microscope (SEM) photo, and measure the diameter of each fiber from the photo, accurate to 0.1 μm, and then calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0021] In the present invention, polyester fiber refers to polyester fiber having a length greater than 150 mm.

[0022] According to the present invention, the melting point of the core layer material is more than 20°C higher than the melting point of the skin layer material (for example, 20°C, 22°C, 24°C, 25°C, 30°C, 40°C, 50°C, 60°C, 80°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, and a range formed by any two of the above points, for example 20-160°C). Preferably, the melting point of the core layer material differs from that of the skin layer material by more than 24°C.

[0023] According to the present invention, preferably, the intrinsic viscosity of the skin material is 0.01-0.18 dL / g higher than that of the core material, and the standard deviation of the intrinsic viscosities of the core material and the skin material is ≤0.015 dL / g.

[0024] In the present invention, the test method for the intrinsic viscosity of polyester is the intrinsic viscosity test method described in GBT14190-2017. The test method for the standard deviation of the intrinsic viscosity is: randomly take 6 samples, test the intrinsic viscosity according to the intrinsic viscosity test method described in GBT14190-2017, and take the standard deviation of the values. The smaller the standard deviation of the intrinsic viscosity, the higher the uniformity of the intrinsic viscosity of the same batch of materials.

[0025] According to the present invention, the standard deviation of the intrinsic viscosity of the core layer material and the skin layer material can be independently 0.015dL / g, 0.01dL / g, 0.009dL / g, 0.008dL / g, 0.007dL / g, 0.006dL / g, 0.005dL / g, 0.004dL / g, 0.003dL / g, 0.002dL / g, 0.001dL / g, 0.0001dL / g, and the range formed by any two of the above points, for example 0.0001-0.015dL / g.

[0026] According to the present invention, preferably, the shape of the spinneret holes is concentric circles, the difference between the maximum diameter and the minimum diameter of each inner circle of the spinneret holes is ≤2 μm, and the difference between the maximum diameter and the minimum diameter of each outer circle is ≤2 μm. The difference between the maximum diameter and the minimum diameter of each inner circle or each outer circle can be independently 0.1 μm, 0.5 μm, 1.5 μm, 2 μm, and the range formed by any two of the above points, such as 0.1-2 μm.

[0027] In the present invention, it can be understood that the core layer material forms the core layer structure of the polyester fiber after passing through the inner circle of the spinneret hole, and the skin layer material forms the skin layer structure of the polyester fiber after passing through the outer circle of the spinneret hole, and then after cooling and stretching, a polyester fiber with a skin-core structure is formed.

[0028] According to the present invention, preferably, the inner diameter of the inner circle of the spinneret is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm. In the present invention, the precise aperture of the spinneret is measured by a profilometer.

[0029] According to the present invention, preferably, the core layer material is melted at 220-280° C. to obtain the molten core layer material.

[0030] According to the present invention, preferably, the skin layer material is melted at 200-280° C. to obtain the melted skin layer material.

[0031] In the present invention, it is understood that the temperature of the core layer material melt and the skin layer material melt refers to the temperature of the spinneret when the core layer material melt and the skin layer material melt are extruded from the spinneret outlet.

[0032] According to the present invention, preferably, the core layer material is homopolyethylene terephthalate.

[0033] According to the present invention, preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.6-0.8 dL / g (for example, 0.6 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.8 dL / g, and a range consisting of any two of the above points), more preferably 0.65-0.7 dL / g, and the standard deviation of the intrinsic viscosity is ≤0.015 dL / g.

[0034] According to the present invention, preferably, the melting point of the homopolyethylene terephthalate is above 240°C (for example, 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and a range consisting of any two of the above points), more preferably 245-265°C.

[0035] According to the present invention, preferably, the skin layer material is co-polyethylene terephthalate.

[0036] According to the present invention, preferably, the intrinsic viscosity of the copolyethylene terephthalate is 0.5-0.9 dL / g (for example, 0.5 dL / g, 0.52 dL / g, 0.54 dL / g, 0.60 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.86 dL / g, 0.88 dL / g, 0.9 dL / g, and a range formed by any two of the above points), more preferably 0.6-0.8 dL / g, and the standard deviation of the intrinsic viscosity is ≤0.015 dL / g.

[0037] According to the present invention, preferably, the melting point of the copolyethylene terephthalate is below 240°C (for example, 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and a range formed by any two of the above points), more preferably 130-240°C.

[0038] According to the present invention, preferably, the copolyethylene terephthalate comprises structural units from terephthalic acid, ethylene glycol and comonomer Y, and the content of the structural units from comonomer Y in the copolyethylene terephthalate is 0.6-18 mol% (for example, 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, and the range composed of any two of the above points). In the present invention, the content of the structural unit of comonomer Y refers to: the percentage of the amount of the structural unit of comonomer Y to the total amount of the structural units of terephthalic acid, ethylene glycol and comonomer Y.

[0039] According to the present invention, the type of the comonomer is not particularly limited, as long as the melting point and intrinsic viscosity difference between the skin material and the core material and the standard deviation of the intrinsic viscosity can meet the requirements. Preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols and tetraols. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, and is more preferably isophthalic acid and / or phthalic acid. More preferably, the diol is a diol having a carbon number of 3-20, and is more preferably at least one of butanediol, hexanediol, cyclohexanedimethanol and pentanediol (e.g., neopentanediol). More preferably, the tetraol has a carbon number of 3-20. In the present invention, the carbon number of the diol or tetraol can be 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20 independently of each other.

[0040] In the present invention, the comonomer Y may also be selected from acid monomers in other forms such as anhydrides, acid chlorides, methanol esters, and ethanol esters, and the alcohol monomers include alcohol monomers in other forms such as ethers, hemiacetals, and acetals.

[0041] According to the present invention, preferably, the mass ratio of the core layer material to the skin layer material is x, and 1.5≤x≤4.

[0042] In the present invention, the amount of the core layer material is higher than that of the skin layer material, and the mass ratio (weight ratio) of the core layer material to the skin layer material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.2, 3.5, 3.8, 4, and a range consisting of any two of the above points, preferably 2-3.6. Limiting the weight ratio of the core layer material to the skin layer material within the above preferred range can further improve the uniformity of the thickness of the polyester fiber, that is, obtain a smaller standard deviation of the polyester fiber diameter.

[0043] According to the present invention, preferably, the mass ratio x of the core layer material to the skin layer material and the average diameter D of the polyester fiber satisfy the relationship of formula (1):

[0044]

[0045] According to the present invention, the inventors have further discovered that when a fiber cloth prepared from polyester fibers whose mass ratio x of the core layer material to the skin layer material and the average diameter D of the polyester fibers satisfy the relationship of the following formula (1) is used for cell culture, it can improve the sugar metabolism capacity of cells.

[0046] More preferably, when the mass ratio of the core layer material to the skin layer material of the composite polyester fiber is 2-3 and satisfies the formula (1), the sugar metabolism capacity of cells can be further improved when the composite polyester fiber is used for cell culture.

[0047] According to the present invention, preferably, the wind speed of the side blowing is 1.5-1.8 m / s (for example, 1.5 m / s, 1.6 m / s, 1.7 m / s, 1.8 m / s, and a range formed by any two of the above points), and the temperature is 15-20°C.

[0048] According to the present invention, preferably, the spacing of the slits is 0.3-3 cm and the width is 10-60 cm. It is understood that the width of the slits refers to the distance of the slits parallel to the long side direction of the spinneret. The length of the slits can be 10-60 cm. The length of the slits refers to the distance along the direction of fiber ejection.

[0049] According to the present invention, preferably, a high-speed airflow is used in the slit to stretch the spun fibers, wherein the flow rate of the high-speed airflow is 2500-5500 m / min.

[0050] According to the present invention, preferably, the temperature of the hot pressing is 180-220°C.

[0051] According to the present invention, preferably, the Sb content in the core layer material and the skin layer material of the polyester fiber is less than 20 ppm. In the present invention, "ppm" refers to the "weight" content.

[0052] The second aspect of the present invention provides a polyester fiber cloth prepared by the method described above.

[0053] The third aspect of the present invention provides a polyester fiber cloth, which is made of polyester fibers with a skin-core structure and self-bonded, and the thickness of the polyester fiber cloth is 0.4-0.5mm, and the surface density is 80-130g / m 2 ; Among them, the average diameter of polyester fiber is 17-32μm, and the standard deviation of diameter is ≤2.2μm.

[0054] In the present invention, the core layer material and the skin layer material in the polyester fiber are as described above and will not be described in detail here.

[0055] The fourth aspect of the present invention provides use of the above-mentioned polyester fiber cloth in cell culture.

[0056] According to the present invention, preferably, the application includes increasing the sugar metabolism rate of cells. Preferably, using the fiber cloth of the present invention for cell culture can increase the sugar metabolism rate of cells to above 15 g / day, for example, 15-30 g / day.

[0057] The present invention will be described in detail below through examples.

[0058] Unless otherwise specified, the spinneret is manufactured by Changzhou Jier Precision Machinery Manufacturing Co., Ltd. and is customized as required.

[0059] The Sb content in the core layer material and the skin layer material is less than 20 ppm.

[0060] The room temperature is about "15-20℃".

[0061] In the following embodiments, the homemade polyester involved is prepared by mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and an optional comonomer Y (e.g., cyclohexanedimethanol, analytical grade) in a reactor according to the required amount of the product, heating to 258-263° C., reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290° C., and evacuating with an oil pump, reacting for about 2 hours to obtain the desired PET.

[0062] The test method for the average diameter of polyester fibers is as follows: randomly select 50 polyester fibers, cut a small section at random, take scanning electron microscope (SEM) photos, and measure the diameter of each fiber from the photos, accurate to 0.1μm, and then calculate the average value of these diameter values ​​according to the average value calculation method; and calculate the standard deviation of these diameter values ​​according to the standard deviation calculation method.

[0063] Example 1

[0064] Homopolyethylene terephthalate (manufacturer: Sinopec Yizheng Chemical Fiber Co., Ltd., hereinafter referred to as "Yizheng Chemical Fiber", model FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261°C) was used as the core material. Copolyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, comonomer cyclohexanedimethanol, comonomer content 15 mol%, melting point 140°C) was used as the skin material. The mass ratio of the core material to the skin material was 3, and the core material was extruded from a spinneret at an outlet temperature of about 280°C. Among them, there are 48 small holes with skin-core concentric structure distributed on the spinneret, the inner diameter of the inner hole is 20 microns, the range (the diameter of the largest hole minus the diameter of the smallest hole) is 1.2 microns, and the inner diameter of the outer hole is 30 microns, and the range is 1.1 microns. After the polymer melt is extruded from the spinneret, it is blown and cooled with room temperature wind of 1.5-1.8m / s, and the cooled fiber is sent into a slit with a spacing of 1cm, the width of the slit is 50cm, and the length is 50cm. The cooled filaments are stretched at a wind speed of 3500-4000m / min to obtain polyester fibers. The average diameter of the polyester fiber is tested and the diameter standard deviation is calculated. The test results are shown in Table 1.

[0065] The polyester fiber is laid on a web laying machine, and then the fiber cloth of a certain thickness is hot pressed at 190-200℃ using a non-embossed polished hot roller to obtain a fiber cloth of a specific thickness. The test method for the surface density of the fiber cloth is: use a 1 / 10,000 balance to measure the surface density of 1cm 2 The fiber cloth of the area is weighed, and then the mass of the fiber cloth is divided by its area; the test method of the fiber cloth thickness is: using a dial thickness gauge (model 547-313) from Suzhou Quantum Instrument Co., Ltd. The surface density and thickness of the fiber cloth are shown in Table 1.

[0066] Example 2

[0067] The method of Example 1 was followed, except that copolyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol%, melting point 237°C) was used as the skin material.

[0068] Example 3

[0069] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 2, the inner diameter of the inner hole of the spinneret used is 18 microns, the range is 1.1 microns, and the inner diameter of the outer hole is 32 microns, the range is 1.6 microns.

[0070] Example 4

[0071] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 3.6.

[0072] Example 5

[0073] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.6.

[0074] Comparative Example 1

[0075] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 1.

[0076] Comparative Example 2

[0077] The method of Example 1 is followed, except that the mass ratio of the core layer material to the skin layer material is 5.

[0078] Comparative Example 3

[0079] The method of Example 1 was followed, except that the raw material used for homopolyethylene terephthalate was homemade polyester, the intrinsic viscosity was 0.51 dL / g, the standard deviation of the intrinsic viscosity was 0.01 dL / g, and the melting point was 260°C.

[0080] Comparative Example 4

[0081] The method of Example 1 was followed, except that the holes of the spinneret were not concentric circles, but parallel structures that divided the circle into two. The inner diameter of the circular spinneret holes was 30 μm, and the range was 1.1 μm.

[0082] Comparative Example 5

[0083] The method of Example 1 is followed, except that the spinneret has a larger aperture range, with the inner diameter of the inner layer hole being 20 μm and the range being 3.6 μm, and the inner diameter of the outer layer hole being 30 μm and the range being 5.3 μm.

[0084] Comparative Example 6

[0085] The method of Example 1 was followed, except that the raw material model of the homopolyethylene terephthalate used was BG85 from Yizheng Chemical Fiber, with an intrinsic viscosity of 0.879 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0086] Comparative Example 7

[0087] The method of Example 1 is followed, except that the raw material used for homopolyethylene terephthalate is homemade polyester, with an intrinsic viscosity of 0.675 dL / g, a standard deviation of the intrinsic viscosity of 0.031 dL / g, and a melting point of 260°C; the raw material used for copolyethylene terephthalate is homemade polyester, with an intrinsic viscosity of 0.782 dL / g, a standard deviation of the intrinsic viscosity of 0.025 dL / g, and a melting point of 143°C, and the comonomer is cyclohexanedimethanol, with a comonomer content of 15 mol%.

[0088] Comparative Example 8

[0089] The method of Example 1 was followed, except that the raw material model of the homopolyethylene terephthalate used was BG804 from Yizheng Chemical Fiber, the intrinsic viscosity was 0.801 dL / g, the standard deviation of the intrinsic viscosity was 0.005 dL / g, and the melting point was 237°C.

[0090] Comparative Example 9

[0091] The method of Example 1 was followed, except that the raw material model of the co-polyethylene terephthalate was BG85 from Yizheng Chemical Fiber, the intrinsic viscosity was 0.879 dL / g, the standard deviation of the intrinsic viscosity was 0.005 dL / g, and the melting point was 248°C.

[0092] Comparative Example 10

[0093] The method of Example 1 is followed, except that the wind speed in the slit is 1500-2000 m / min.

[0094] Comparative Example 11

[0095] The method of Example 1 is followed, except that after the molten filament is cooled, it does not enter the slit, but is moderately stretched and laid into a web using the swing wire method.

[0096] Test Example 1

[0097] Pretreatment and sterilization of fiber cloth: Soak the fiber cloth in a 7 wt% hydrogen peroxide aqueous solution at room temperature for 60 minutes; then use ultrapure water to ultrasonically clean the fiber cloth, and then dry it at 80°C. After drying, sterilize the fiber cloth with high-temperature steam at a temperature of 121°C for 60 minutes.

[0098] Then the sugar metabolism ability of Vero cells was tested when the sterilized fiber cloth was used as a carrier. The test method for sugar metabolism is: cut the fiber cloth into small pieces of 6mm×6mm fiber cloth, place 100g of 6mm×6mm fiber cloth, 8L PBS buffer and about 200mL of 199 culture medium (purchased from Beijing Tsinghua Tianyi Biotechnology Co., Ltd.) in a 10L basket reactor, add 10% high-quality newborn calf serum (purchased from Lanzhou Minhai Biotechnology Co., Ltd.) relative to the culture medium, and add an appropriate amount of 20% by weight concentration of glucose so that the total glucose concentration is 0.5% by weight. Inoculate Vero cells (purchased from the National Biomedical Experimental Cell Resource Bank, 4th generation Vero cells, every 1cm 2 The inoculation amount of cells on the fiber cloth was 0.5×10 6 ), aeration culture at 37°C, pH 7.3, DO (percentage of dissolved oxygen in saturated dissolved oxygen) 60%, 5% carbon dioxide concentration, and slow stirring during culture for 7 days. Samples were taken every 24 hours and the glucose content was detected using a glucose detection kit (purchased from Nanjing Jiancheng Bioengineering Institute), and the glucose concentration was supplemented to 0.5% by weight with a 20% by weight glucose solution. The measured reduction in glucose content was converted into a glucose consumption rate value in g / day, and a curve of glucose consumption rate versus culture time was drawn, and then the value at 100h was taken as the 100h sugar metabolism rate value. The 100h sugar metabolism rate value is shown in Table 1.

[0099] Table 1

[0100]

[0101] The preferred embodiments of the present invention are described in detail above, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various technical features in any other suitable manner, and these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A method for preparing a polyester fiber cloth, characterized in that: The method includes: (1) feeding the melted core layer material and the skin layer material into a spinneret for spinning to obtain a filamentary melt having a skin-core structure; (2) cooling the filament melt having a skin-core structure to obtain a primary fiber; (3) stretching the spun fiber in a slit to obtain a polyester fiber with a skin-core structure; (4) Polyester fibers with an average diameter of 17-32 μm and a diameter standard deviation of ≤2.2 μm are laid and hot-pressed to obtain polyester fiber cloth.

2. The method according to claim 1, wherein: The core layer material is homopolyethylene terephthalate; Preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.6-0.8 dL / g, the standard deviation of the intrinsic viscosity is ≤0.015 dL / g, and the melting point is above 240°C.

3. The method according to claim 1, wherein: The skin material is copolyethylene terephthalate; Preferably, the intrinsic viscosity of the co-polyethylene terephthalate is 0.5-0.9 dL / g, the standard deviation of the intrinsic viscosity is ≤0.015 dL / g, and the melting point is below 240°C.

4. The method according to claim 3, wherein: The copolyethylene terephthalate comprises structural units derived from terephthalic acid, ethylene glycol and comonomer Y, and the content of the structural units derived from comonomer Y in the copolyethylene terephthalate is 0.6-18 mol%; More preferably, the comonomer Y is selected from at least one of dicarboxylic acids, diols and tetraols.

5. The method according to claim 1, wherein: The mass ratio of the core material to the skin material is x, 1.5≤x≤4; And / or, the mass ratio x of the core layer material to the skin layer material and the average diameter D of the polyester fiber satisfy the relationship of formula (1):

6. The method according to claim 1, wherein: The spacing of the slits is 0.3-3 cm, the width is 10-60 cm, and the length is 10-60 cm; And / or, a high-speed airflow is used in the slit to stretch the spun fibers, wherein the flow rate of the high-speed airflow is 2500-5500 m / min.

7. The method according to claim 1, wherein: The temperature of the hot pressing is 180-220°C.

8. The polyester fiber cloth prepared by the method according to any one of claims 1 to 7.

9. A polyester fiber cloth, characterized in that: The polyester fiber cloth is made of polyester fibers with a skin-core structure and is self-adhesive. The thickness of the polyester fiber cloth is 0.4-0.5 mm and the surface density is 80-130 g / m 2 ; Among them, the average diameter of the polyester fiber is 17-32μm, and the standard deviation of the diameter is ≤2.2μm.

10. Use of the polyester fiber cloth according to claim 8 or 9 in cell culture.

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