A composition for improving mitochondrial activity and activating cell energy, and its preparation and medical and cosmetic applications

Abstract

The present invention discloses a composition for improving mitochondrial activity and activating cell energy, and its preparation and medical and cosmetic applications. The composition includes: citrulline, sodium DNA, taurine, arginine / lysine polypeptide, coenzyme Q10, NAD+, creatine, adenosine, ATP, glutamine, inositol, carnosine, glutathione, etc. The present invention activates cells and replenishes energy to improve skin cell vitality from the aspects of improving skin microcirculation, activating mitochondrial function, supplementing nutrition, and maintaining cell metabolism stability through a specific combination. The composition is applied in dermatology to provide energy for important cell functions such as cell renewal and wound repair, and accelerate wound healing; it is applied in skin beauty to fill skin cells with energy, and can effectively improve the volume, depth and area of ​​crow's feet, improve skin roughness, and improve skin elasticity, firmness and glossiness.

Description

Technical Field

[0001] The present invention belongs to the field of medicine and cosmetic technology, and in particular, relates to a composition for improving mitochondrial activity and activating cell energy, and its preparation, and medical and cosmetic applications. Background Art

[0002] Skin aging is closely related to the production and maintenance of cellular energy. As we age, the energy metabolism of skin cells changes, resulting in reduced energy production, which directly affects the activation and repair ability of cells. Skin microcirculation, mitochondrial function, nutritional supplementation, and cellular metabolic stability will affect the transfer of cellular energy, which in turn affects skin health.

[0003] The flow of blood, lymph and tissue fluid that directly participates in the transfer of substances, information and energy of tissues and cells is called microcirculation. In clinical practice, microcirculation is generally considered to refer to the blood microcirculation in capillaries. Capillaries are the most important part for tissue cells to exchange substances. Microvessels are the pathways of circulation. Except for part of the blood that flows through arteriovenous shunts, almost all of the circulating blood in the body flows through microvessels to perfuse tissues and cells. Tissue fluid exists between tissues and cells, and flows between microvessels, cells and capillary lymphatic vessels. Capillary lymphatic vessels are one of the important output channels for cells and tissues. Therefore, microcirculation is the place where cells, tissues exchange substances with blood and lymph. If the skin microcirculation is impaired and the blood vessels are blocked, the exchange of substances between blood, lymph and cell tissues will not be smooth or cannot be carried out.

[0004] Cellular energy metabolism, especially the intracellular tricarboxylic acid cycle (TCA cycle) and oxidative phosphorylation (OXPHOS), is essential for cell function and tissue repair. These metabolic processes not only provide cells with the necessary energy (ATP), but also participate in maintaining cell biosynthesis and cell biological activity.

[0005] As the main energy production factory in cells, mitochondria are the place where cells perform aerobic respiration (biological combustion), and sugars, fats and amino acids are finally oxidized to release energy. Mitochondria synthesize ATP, providing more than 90% of the energy required for cell activities. Its functional state directly affects the energy metabolism of cells. Mitochondria are involved in many cellular physiological processes: cell differentiation, cell growth, cell information transmission, cell cycle, cell apoptosis; mitochondria regulate the active functions of tissues and organs: energy metabolism, oxidative stress, inflammation, autophagy, calcium homeostasis, tissue reconstruction, muscle development; mitochondria affect various aspects of the body's functions: immunity, physical strength, memory, thinking vitality, emotions; mitochondrial dysfunction causes related diseases: injury, aging, metabolic disorders, muscle degeneration, cancer, diabetes, heart disease, neurodegenerative diseases.

[0006] Changes in mitochondrial quality and activity (morphology, structure, quantity and mitochondrial dynamics) and the biosynthesis of the electron transport chain complex directly affect cell aging. Damaged and inefficient mitochondria → insufficient ATP production → excessive ROS and free radicals → cytotoxicity → cell damage → accelerated aging. Decreased mitochondrial function will lead to problems including imbalance of intracellular energy metabolism, increased reactive oxygen species (ROS), cell cycle arrest, secretion of multiple inflammatory factors, and accelerate cell aging. The accumulation of senescent cells directly manifests as skin aging.

[0007] Therefore, improving the energy metabolism of skin cells, remodeling mitochondrial function, regulating energy balance, and maintaining metabolic stability are new ideas for restoring the repair capacity of cells and promoting aging and damage repair. This suggests that regulating energy metabolism and mitochondrial function may help delay skin aging and improve its repair capacity.

[0008] Energy is a necessary condition for life to function, and cells need energy through metabolic activity. Intake of all necessary nutrients helps meet the nutritional needs of cells and supports the four basic mechanisms of cellular and overall health. Cells break down the structure of nutrient molecules to release energy.

[0009] Cell metabolism is the sum of a series of chemical reactions that occur in organisms, including anabolism, catabolism, and mixed metabolism. Anabolism is the process of synthesizing molecules in organisms, including protein synthesis, nucleic acid synthesis, lipid synthesis, etc. Anabolism usually requires energy input to drive these synthetic reactions. For example, glucose can be converted into lipids or proteins through the anabolism pathway. Catabolism is the decomposition of complex biological macromolecules into simpler molecules while releasing energy. This includes protein degradation, lipid oxidation, carbohydrate decomposition, etc. The energy produced by catabolism can be used by cells for anabolism or to perform other biological functions. Mixed metabolism refers to the simultaneous process of anabolism and catabolism. In cells, these metabolic processes are interrelated to form a dynamic balance. For example, glucose can produce energy (catabolism) through the glycolysis pathway in cells, and can also be used to synthesize lipids or other biological macromolecules (anabolism).

[0010] Cell metabolism provides the energy, raw materials and signaling molecules required by cells, affecting the physiological state and function of cells. In terms of energy supply, cell metabolism produces ATP, which is the main source of energy in cells and is essential for various functional activities of cells, such as cell division, signal transduction and protein synthesis. In terms of anabolic substances, metabolism provides the biological molecules required to build cells, such as proteins, nucleic acids, lipids, etc., which are essential for maintaining cell structure and function. In addition, in terms of the generation of signal molecules, the metabolites produced by metabolic pathways act as signaling molecules in cells, regulating the cell's signal transduction network, thereby affecting the fate of cells. In addition, metabolites can also regulate the cell's microenvironment, affect the cell's acid-base balance, redox state and ion balance, and have an important impact on cell function and fate, especially during cell differentiation and tissue development. Finally, the interaction between metabolism and the epigenome causes intermediates to affect the modification state of DNA and proteins, directly affecting the expression level of genes and the function of cells. At the same time, the flexibility of cell metabolism helps maintain the homeostasis and balance of cells, enabling cells to adapt to different physiological conditions. These factors together determine the significant impact of cell metabolism on cell fate.

[0011] Cellular homeostasis is regulated by the coordinated activity of several key metabolic pathways. These processes, including carbohydrate metabolism, lipid metabolism, glutamine metabolism, and nucleotide metabolism, maintain the energy status of the cell and provide essential components to ensure proper cellular function. These metabolic pathways are regulated by extracellular signaling.

[0012] Carbohydrate Metabolism: Carbohydrate metabolism encompasses all biochemical processes responsible for the formation, breakdown, and interconversion of carbohydrates (molecules composed of carbon, hydrogen, and oxygen (CHO)) to ensure a constant supply of energy to living cells. Glucose, the major substrate for metabolism, is absorbed into the bloodstream by the small intestine and circulates to all tissues in the body, where uptake is regulated by insulin signaling to provide the majority of an individual's daily energy requirements. Glucose is further broken down into pyruvate by a process called glycolysis, which results in the net production of adenosine triphosphate (ATP), an important energy source for living cells. ATP is converted to the polysaccharide glycogen by glycogenesis primarily in the liver and skeletal muscle, where it serves as an emergency fuel reserve that can be released as free glucose by glycogenolysis.

[0013] Lipid Metabolism: Lipid metabolism includes biological processes involved in the synthesis or degradation of lipids, which are a class of organic compounds that include fatty acids or their derivatives that are insoluble in water but soluble in organic solvents. Lipids serve as building blocks for key cellular structures such as membranes, play a role in many cellular signaling networks, and are a source of energy-rich fuel used to support cellular function. Complex lipids known as triglycerides are broken down by enzymes called lipases during digestion in the mouth and small intestine and are transported in the blood by lipoproteins. Fatty acids serve as both a source of energy and a unit of energy storage in cells. Fatty acids are synthesized in the cytoplasm of cells from acetyl-CoA and NADPH in a process catalyzed by fatty acid synthases and are subsequently metabolized into phospholipids that serve as major components of cell membranes and also play a role in cell signaling pathways.

[0014] Glutamine Metabolism: The amino acid glutamine is an important metabolic fuel for rapidly proliferating cells. Glutamine is the most abundant free amino acid in the circulation and in intracellular fluids. It serves as a substrate to meet the increasing demand for ATP, biosynthetic precursors, and reducing agents in dividing cells. Specific amino acid transporters allow glutamine to enter the cell where it is then converted in the mitochondria to glutamate, a precursor of the TCA cycle intermediate alpha-ketoglutarate. Cancer cells often rely on glutamine metabolism to meet their increased energy demands.

[0015] Nucleotide metabolism: is a series of biochemical reactions required for the synthesis and degradation of the basic building blocks of nucleic acids, DNA and RNA. The two major groups of nucleotides are purines (adenine and guanine) and pyrimidines (cytidine, uridine and thymidine). All nucleotides are composed of a pentose sugar and a phosphate group, but purines and pyrimidines differ in the size of the nitrogen base. Nucleotides in the form of nucleoside triphosphates (ATP, GTP, CTP and UTP) serve as a store of chemical energy required for many cellular functions, including amino acid and protein synthesis, cell migration and division, which is released by the removal of phosphates. The cyclic nucleotides cGMP and cAMP serve as key second messengers in many cellular signaling cascades that are modified by a class of enzymes called cyclic nucleotide phosphodiesterases.

[0016] However, the existing technology tends to supplement cells with high-energy nutrients, but does not comprehensively solve the problems of cell energy metabolism and mitochondrial function from the perspectives of accelerating cell energy absorption and metabolism, maintaining efficient and stable cell metabolism, and helping cells clean up metabolic waste. In addition, the existing technology does not solve the problem of skin penetration well, and is difficult to be absorbed and utilized by skin cells. Summary of the invention

[0017] The primary purpose of the present invention is to provide a composition for improving mitochondrial activity and activating cell energy, as well as its preparation and medical and cosmetic applications, so as to activate cells to replenish energy and improve skin cell vitality through a specific combination from the aspects of improving skin microcirculation, activating mitochondrial function, supplementing nutrition, and maintaining cell metabolic stability.

[0018] To this end, the present invention provides the following technical solutions.

[0019] One aspect of the present invention provides a composition for improving mitochondrial activity and activating cellular energy. The composition comprises the following components in parts by weight: 0.0001-3 parts of citrulline, 0.0001-3 parts of sodium DNA, 0.0001-3 parts of taurine, 0.0001-2 parts of arginine / lysine polypeptide, 0.0001-2 parts of coenzyme Q10, 0.0001-2 parts of NAD+, 0.0001-0.5 parts of creatine, 0.0001-2 parts of adenosine, 0.0001-2 parts of ATP, 0.0001-2 parts of glutamine, 0.0001-1 parts of inositol, 0.0001-2 parts of carnosine, and 0.0001-2 parts of glutathione.

[0020] In a preferred embodiment of the present invention, the composition comprises the following components in parts by weight: 1-3 parts of citrulline, 1-3 parts of sodium DNA, 1-3 parts of taurine, 1-2 parts of arginine / lysine polypeptide, 1-2 parts of coenzyme Q10, 1-2 parts of NAD+, 0.1-0.5 parts of creatine, 1-2 parts of adenosine, 1-2 parts of ATP, 1-2 parts of glutamine, 0.1-1 parts of inositol, 1-2 parts of carnosine, and 1-2 parts of glutathione.

[0021] In a preferred embodiment of the present invention, the composition further comprises the following components in parts by weight: 0.0001-3 parts of ginsenoside Rh2, 0.0001-3 parts of soybean polypeptide, 0.0001-2 parts of β-sitosterol and 0.0001-1 parts of calcium pantetheine sulfonate.

[0022] In a preferred embodiment of the present invention, the composition further comprises the following components in parts by weight: 1-3 parts of ginsenoside Rh2, 1-3 parts of soybean polypeptide, 1-2 parts of β-sitosterol and 0.1-1 parts of calcium pantetheine sulfonate.

[0023] Another aspect of the present invention provides a skin care product for improving mitochondrial activity and activating cell energy, wherein the skin care product comprises the composition for improving mitochondrial activity and activating cell energy as described above.

[0024] In a preferred embodiment of the present invention, the skin care product comprises the following components in weight percentage: 1-5% of a composition for improving mitochondrial activity and activating cell energy, 1-5% of a surfactant, 1-3% of a fatty acid, 2-6% of a phospholipid, 1-5% of an additive and the remainder of water.

[0025] In a preferred embodiment of the present invention, the surfactant is selected from one or a combination of cetearyl glucoside, sorbitan olivate, polysorbate-80, polysorbate-60, ceteareth-2, polyglyceryl-10 laurate, polyoxyethylene castor oil, and polyoxyethylene sorbitan monooleate.

[0026] In a preferred embodiment of the present invention, the fatty acid is selected from one or a combination of sunflower seed oil, avocado oil, meadowfoam seed oil, palmitic acid, linoleic acid, and linolenic acid.

[0027] In a preferred embodiment of the present invention, the phospholipid is selected from one or a combination of lecithin, dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, and distearoylphosphatidylcholine.

[0028] In a preferred embodiment of the present invention, the additives include 1,2-pentanediol, rose essential oil, disodium EDTA and glycerin.

[0029] In a preferred embodiment of the present invention, the additive further comprises a pH regulator, and the pH regulator is selected from one or a combination of citric acid, malic acid, potassium hydroxide, arginine, and tromethamine.

[0030] Another aspect of the present invention also provides a method for preparing the skin care product for improving mitochondrial activity and activating cell energy as described above, the method comprising the following steps:

[0031] Prepare the aqueous phase: stir deionized water, 1,2-pentanediol, glycerol, disodium EDTA and a water-soluble surfactant in an aqueous phase pot to obtain an aqueous phase;

[0032] Prepare the oil phase: stir the fatty acid, the composition, the oil-soluble surfactant and the phospholipid in an oil phase pot to obtain the oil phase;

[0033] The aqueous phase and the oil phase are mixed and the pH of the system is adjusted to 6.9-7.3 using a pH adjuster, and then rose essential oil is added, and ultrasonic homogenization and emulsification are performed at a power of 500-1000 W for 5-10 minutes to obtain a nanoemulsion with an average particle size between 50-100 nm, which is the resulting skin care product.

[0034] By means of the above technical solution, the present invention has at least the following advantages:

[0035] 1. The present invention uses ginsenoside Rh2, soybean polypeptide, β-sitosterol and calcium pantethiothioate as the main active substances for improving mitochondrial activity and activating cell energy. Ginsenoside Rh2 is one of the extracts of ginseng and belongs to triterpenoid glycoside compounds. Studies have shown that ginsenoside Rh2 monomer has an inhibitory effect on the growth of cancer cells, can induce tumor cell apoptosis, reverse abnormal differentiation of tumor cells, and resist tumor metastasis. It can play a role in enhancing efficacy and reducing toxicity when used in combination with chemotherapy drugs. In addition, ginsenoside Rh2 also has the effects of improving body immunity, antibacterial, improving cardiovascular and cerebrovascular insufficiency, regulating the central nervous system, anti-fatigue, and delaying aging. Soybean (GLYCINE MAX) polypeptide comes from soybeans and is soybean protein. It is hydrolyzed, separated, and purified by protease to obtain a product rich in polypeptides. Soybean polypeptide is a low-peptide mixture composed of 3 to 6 amino acid residues after the hydrolysis of soybean protein, with a molecular weight of about 1000. It can promote the growth of fibroblasts, and has the effects of skin rejuvenation and anti-aging; at the same time, it can keep the skin hydrated and has a moisturizing effect. β-Sitosterol belongs to plant sterols, which inhibits the activity of metalloproteinases, has obvious anti-inflammatory properties, and can be used to treat skin ulcers and skin tumors; β-Sitosterol has antioxidant properties and the like, and is used in skin care products in combination with vitamin nutrients, has conditioning effects, can delay and treat skin keratinization, and keep the skin smooth and moist. β-Sitosterol is easily compounded with lecithin to form liposomes, which will be beneficial to the transdermal penetration of sitosterol. Calcium pantethione sulfonate is a skin conditioning agent and antioxidant. It has the effect of scavenging free radicals, that is, helping the skin to resist oxidation and beautify the skin. The present invention has found through research results that the combined action of four substances, ginsenoside Rh2, soybean polypeptide, β-sitosterol and calcium pantethione sulfonate, can significantly improve the anti-wrinkle and whitening effects of skin care products.

[0036] 2. The composition of the present invention also contains citrulline, sodium DNA, taurine, arginine / lysine polypeptide and other ingredients. Citrulline is a precursor of nitric oxide, which is a vasodilator that can increase blood circulation, improve the delivery of oxygen and energy substances, and help skin cells get sufficient energy supplement. Arginine / lysine polypeptide (a new type of cyclic peptide derived from bone morphogenetic protein 2): BMP-2 peptide segment, cyclic peptide, 1-5 minutes of efficient penetration into cells, specific binding to mitochondria, strengthening sugar metabolism, promoting ATP production, increasing cell energy supply, efficiently stimulating cell vitality, and improving cell basal metabolic rate. Inositol, as a lipotropic agent, can accelerate the decomposition of fat, prevent abnormal or excessive accumulation of fat, and increase the utilization rate of fat in the body; it also helps the metabolism of fatty acids and carbohydrates; it is a substance necessary for the integrity of cell membranes; it may help the conduction of nerve signals; it is lipophilic; it can control the calcium concentration in cells; it is a necessary substance for the optimal function of insulin. Inositol can promote cell regeneration and repair damaged skin. Long-term use can improve skin texture and make the skin more delicate and smooth. Creatine is a nitrogen-containing organic acid with the chemical formula C4H9N3O2 and the scientific name N-methylguanidinoacetic acid. It exists naturally in vertebrates and can help provide energy for muscles and nerve cells. Carnosine (including carnosine or decarboxylated carnosine hydrochloride) acts on all skin cells and extracellular tissues to scavenge free radicals, chelate heavy metal ions, and bind reducing sugars. Taurine is an amino acid converted from sulfur-containing amino acids. It is widely distributed in various tissues and organs in the body and mainly exists in the interstitial fluid and intracellular fluid in a free state. Taurine is widely distributed in the brain, heart, liver, kidney, ovary, uterus, skeletal muscle, blood, saliva and milk of humans and animals in the form of free amino acids. The highest concentration is in the pineal gland, retina, pituitary gland, adrenal gland and other tissues. In the heart of mammals, free taurine accounts for as much as 50% of the total free amino acids. Taurine is an organic osmotic regulator that not only participates in regulating cell volume, but also provides a basis for the formation of bile salts and plays an important role in regulating intracellular free calcium concentration. Many studies have found that taurine is an active substance that regulates the normal physiological activities of the body. Glutathione (GSH) is a tripeptide containing γ-amide bonds and sulfhydryl groups. It is composed of glutamic acid, cysteine ​​and glycine and exists in almost every cell of the body. The GSH molecule is characterized by an active sulfhydryl group (-SH), which is the most important functional group. It can participate in many important biochemical reactions of the body, protect the sulfhydryl groups of important enzyme proteins in the body from oxidation and inactivation, and ensure the normal energy metabolism and cell utilization of cells. Coenzyme Q10 is one of the participants in the electron transport chain and aerobic respiration in the mitochondria of eukaryotic cells. It is a yellow to orange crystalline powder, odorless and tasteless, and easily decomposed by light. Phospholipids, also known as phospholipids and phospholipids, refer to lipids containing phosphoric acid and belong to complex lipids.Phospholipids are the main components of biological membranes and are divided into two categories: glycerophospholipids and sphingomyelins, which are composed of glycerol and sphingosine, respectively. Phospholipids are amphiphilic molecules, with a hydrophilic nitrogen- or phosphorus-containing group at one end and a long hydrophobic (lipophilic) hydrocarbon chain at the other end. Therefore, the hydrophilic ends of phospholipid molecules are close to each other, and the hydrophobic ends are close to each other. They often form a phospholipid bilayer, i.e., the structure of the cell membrane, together with other molecules such as proteins, glycolipids, and cholesterol. Adenosine is an endogenous nucleoside that is distributed throughout human cells and is composed of adenine and ribose. It plays an important biological role in cells, including energy conversion and signal transmission. Adenosine is an important endogenous signaling molecule that can act as a ligand and participate in the regulation of various physiological and pathological processes such as cell apoptosis, cell proliferation, vasodilation, cardiac rhythm, and inflammation by binding to G protein-coupled receptors (ADORA1, ADORA2A, ADORA2B, ADORA3). It is a hot topic of research today. ATP Adenosine triphosphate, in biochemistry, adenosine triphosphate (ATP) is a nucleotide that acts as a "molecular currency" for intracellular energy transfer, storing and transferring chemical energy. ATP also plays an important role in nucleic acid synthesis. All cells in our body require small molecule adenosine triphosphate (ATP) produced by mitochondria to provide energy for cell metabolism, dynamic changes and growth. Glutamine is an essential amino acid and an important component of culture medium as the main energy source in cell culture. Glutamine is an important metabolic raw material that helps rapidly proliferating cells meet the growing demand for ATP, biosynthetic precursors and reducing agents. DNA sodium: PDRN (Polydeoxyribonucleotides) refers to polydeoxyribonucleotides, a reliable cell growth active substance. PDRN promotes the formation of DNA by producing nucleotides and nucleosides; this reactivates normal cell proliferation and growth patterns, thereby accelerating tissue regeneration and wound healing. The benefits of PDRN include reversing signs of aging, reducing fine lines and wrinkles, enhancing collagen formation, making skin texture more elastic and firm, repairing damage caused by chronic inflammation or prolonged sun exposure, reducing scars, and improving skin hydration and barrier function. Through transdermal co-delivery of multiple ingredients, multi-target activation of cells, replenishment of energy, and increased cell vitality are achieved.

[0037] 3. The compositions of the present invention are combined and proportioned in a specific manner, and ultrasonic microfluidic homogenization technology is used to obtain skin energy complex liposome encapsulation, which solves the problem of skin transdermal absorption. Cells are activated and energy is replenished to improve skin cell vitality from the aspects of improving skin microcirculation, activating mitochondrial function, supplementing nutrition, and maintaining cell metabolism stability. The composition is applied in dermatology to provide energy for important cell functions such as cell renewal and wound repair, and accelerate wound healing; it is applied in skin beauty to fill skin cells with energy, which can effectively improve the volume, depth and area of ​​crow's feet, improve skin roughness, and improve skin elasticity, firmness and glossiness.

[0038] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the preferred embodiments of the present invention are described in detail as follows. DETAILED DESCRIPTION

[0039] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods.

[0041] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0042] Embodiment 1:

[0043] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 2 parts of citrulline, 2 parts of sodium DNA, 2 parts of taurine, 1.5 parts of arginine / lysine polypeptide, 1.5 parts of coenzyme Q10, 1.5 parts of NAD+, 0.3 parts of creatine, 1.5 parts of adenosine, 1.5 parts of ATP, 1.5 parts of glutamine, 0.5 parts of inositol, 1.5 parts of carnosine, 1.5 parts of glutathione, 2 parts of ginsenoside Rh2, 2 parts of soybean polypeptide, 1.5 parts of β-sitosterol and 0.5 parts of calcium pantetheine sulfonate.

[0044] Embodiment 2:

[0045] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 3 parts of citrulline, 1 part of sodium DNA, 1 part of taurine, 2 parts of arginine / lysine polypeptide, 1 part of coenzyme Q10, 2 parts of NAD+, 0.1 part of creatine, 2 parts of adenosine, 1 part of ATP, 2 parts of glutamine, 0.1 part of inositol, 2 parts of carnosine, 0.1 part of glutathione, 3 parts of ginsenoside Rh2, 1 part of soybean polypeptide, 2 parts of β-sitosterol and 0.1 part of calcium pantetheine sulfonate.

[0046] Embodiment 3:

[0047] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 1 part of citrulline, 3 parts of sodium DNA, 3 parts of taurine, 1 part of arginine / lysine polypeptide, 2 parts of coenzyme Q10, 1 part of NAD+, 0.5 parts of creatine, 1 part of adenosine, 2 parts of ATP, 1 part of glutamine, 1 part of inositol, 1 part of carnosine, 2 parts of glutathione, 1 part of ginsenoside Rh2, 3 parts of soybean polypeptide, 1 part of β-sitosterol and 1 part of calcium pantetheine sulfonate.

[0048] Comparative Example 1:

[0049] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 2 parts of citrulline, 2 parts of sodium DNA, 2 parts of taurine, 1.5 parts of arginine / lysine polypeptide, 1.5 parts of coenzyme Q10, 1.5 parts of NAD+, 0.3 parts of creatine, 1.5 parts of adenosine, 1.5 parts of ATP, 1.5 parts of glutamine, 0.5 parts of inositol, 1.5 parts of carnosine, 1.5 parts of glutathione, 2 parts of soybean polypeptide, 1.5 parts of β-sitosterol and 0.5 parts of calcium pantetheine sulfonate.

[0050] Comparative Example 2:

[0051] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 2 parts of citrulline, 2 parts of sodium DNA, 2 parts of taurine, 1.5 parts of arginine / lysine polypeptide, 1.5 parts of coenzyme Q10, 1.5 parts of NAD+, 0.3 parts of creatine, 1.5 parts of adenosine, 1.5 parts of ATP, 1.5 parts of glutamine, 0.5 parts of inositol, 1.5 parts of carnosine, 1.5 parts of glutathione, 2 parts of ginsenoside Rh2, 2 parts of soybean polypeptide and 0.5 parts of calcium pantetheine sulfonate.

[0052] Comparative Example 3:

[0053] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 2 parts of citrulline, 2 parts of sodium DNA, 2 parts of taurine, 1.5 parts of arginine / lysine polypeptide, 1.5 parts of coenzyme Q10, 1.5 parts of NAD+, 0.3 parts of creatine, 1.5 parts of adenosine, 1.5 parts of ATP, 1.5 parts of glutamine, 0.5 parts of inositol, 1.5 parts of carnosine, 1.5 parts of glutathione, 2 parts of ginsenoside Rh2, 2 parts of soybean polypeptide and 1.5 parts of β-sitosterol.

[0054] Comparative Example 4:

[0055] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 2 parts of citrulline, 2 parts of sodium DNA, 2 parts of taurine, 1.5 parts of arginine / lysine polypeptide, 1.5 parts of coenzyme Q10, 1.5 parts of NAD+, 0.3 parts of creatine, 1.5 parts of adenosine, 1.5 parts of ATP, 1.5 parts of glutamine, 0.5 parts of inositol, 1.5 parts of carnosine, 1.5 parts of glutathione, 2 parts of ginsenoside Rh2, 1.5 parts of β-sitosterol and 0.5 parts of calcium pantetheine sulfonate.

[0056] Comparative Example 5:

[0057] The present embodiment provides a composition for improving mitochondrial activity and activating cellular energy, which includes the following components in parts by weight: 2 parts of citrulline, 2 parts of sodium DNA, 2 parts of taurine, 1.5 parts of arginine / lysine polypeptide, 1.5 parts of coenzyme Q10, 1.5 parts of NAD+, 0.3 parts of creatine, 1.5 parts of adenosine, 1.5 parts of ATP, 1.5 parts of glutamine, 0.5 parts of inositol, 1.5 parts of carnosine and 1.5 parts of glutathione.

[0058] Test 1: Evaluation of the penetration effect of different compositions on the skin

[0059] This experiment explored the evaluation of the penetration effect of different compositions on the skin. The experimental method used was based on the invention patent with publication number CN112957276A, which is incorporated into the present invention by reference. The specific operation is as follows:

[0060] The vertical Franz diffusion cell method was used to conduct a transdermal experiment on ex vivo mouse skin: under a dissecting microscope, undamaged abdominal skin of SD male rats was selected, several pieces of skin of the same size were cut, washed once with PBS, and the surface moisture was absorbed with filter paper, and the skin was fixed on the Franz diffusion cell, with the stratum corneum facing the dosing chamber and the dermis facing the receiving chamber, physiological saline was added to the receiving chamber, and bubbles were removed to ensure that there were no bubbles between the dermis and the receiving solution, the instrument was turned on in advance, the temperature of the water bath was adjusted to 37°C, 10 mg of the composition of Examples 1-3 and Comparative Examples 1-5 was added to the dosing chamber, a sealing film was sealed, tin foil was added to prevent the liquid from evaporating, and the mixture was stirred at 600 rpm. At 12 h, the skin was cut into pieces and ground into a homogenate, extracted, filtered with a 0.22 μm organic membrane, and then tested by HPLC, and the cumulative permeation amount (μg / mL) was tested. The test results are shown in Table 1 below.

[0061] Table 1 Statistics of the cumulative amount of permeation and cumulative retention of different compositions

[0062] Group <![CDATA[Cumulative skin permeation amount (μg / cm 2 )]]> <![CDATA[Cumulative skin retention amount (μg / cm 2 )]]> Example 1 866.9 90.5 Example 2 843.9 83.7 Example 3 854.2 87.1 Comparative Example 1 832.6 80.8 Comparative Example 2 724.9 67.6 Comparative Example 3 768.3 75.9 Comparative Example 4 809.7 81.1 Comparative Example 5 642.4 45.9

[0063] It can be seen from the results in Table 1 that, compared with Comparative Examples 1-5, the compositions of Examples 1-3 of the present invention have higher cumulative skin permeation amounts and cumulative retention amounts.

[0064] Experiment 2: Evaluation of the effects of different skin care products on the skin

[0065] 1. Prepare corresponding skin care lotion according to different compositions

[0066] The composition of the present invention is added to a skin care lotion formula at a concentration of 5%. The formula composition and mass percentage content are shown in Table 2.

[0067] Table 2 Skin care lotion formula

[0068] raw material Mass content (%) Example 1, the composition of comparative examples 1-5 3 Cetearyl Glucoside 0.7 Sorbitan Olivate 2.5 Polysorbate-80 0.5 Polyglyceryl-10 Laurate 1.0 Palmitic acid 1 Avocado Butter 0.5 Linoleic acid 0.5 Dipalmitoylphosphatidylcholine 2 Dipalmitoylphosphatidylethanolamine 2 glycerin 1 1,2-Pentanediol 0.5 Rose essential oil 0.05 Disodium EDTA 0.05 pH Adjusters 0.1 water margin

[0069] The above skin care lotion preparation process is:

[0070] Prepare the aqueous phase: stir deionized water, 1,2-pentanediol, glycerol, disodium EDTA and polysorbate 80 in an aqueous phase pot to obtain an aqueous phase.

[0071] Preparation of oil phase: Cetearyl glucoside, sorbitan olivate, polyglyceryl-10 laurate, palmitic acid, avocado butter, linoleic acid, dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylethanolamine are heated and stirred in an oil phase pot to dissolve uniformly to obtain an oil phase.

[0072] The aqueous phase and the oil phase are mixed and the pH of the system is adjusted to 7.0 using a pH adjuster, and then a composition for improving mitochondrial activity and activating cell energy and rose essential oil are added, and ultrasonic homogenization and emulsification are performed at a power of 1000 W for 10 minutes to obtain a nanoemulsion with an average particle size of 80 nm, which is the obtained skin care lotion.

[0073] The compositions of Example 1 and Comparative Examples 1-5 were prepared according to the above formula and method to obtain skin care lotions corresponding to numbers 1-6 respectively, and subsequent skin tests were carried out.

[0074] 2. Evaluation of the improvement of facial skin by different skin care lotions

[0075] 12 female subjects aged between 25 and 40 were randomly selected and divided into 6 groups, with 2 people in each group. The subjects in groups 1 to 6 used the above-prepared No. 1 to 6 skin care lotions on the face and side arms, respectively, once in the morning and evening every day, and the amount and time of use each time were basically the same. The living and eating habits of the subjects in each group were kept consistent as much as possible. The roughness of the facial skin and the glossiness of the side arm skin were tested before and after 28 days of use, and the data were compared and analyzed before and after to verify the effect of each skin care lotion on improving facial roughness and glossiness.

[0076] 1) The skin fast optical imaging system Evaskin-S5 (EOTECH, France) was used to measure the facial skin roughness Stm and Sa values. The larger the measured value, the rougher the skin.

[0077] 2) Use the Glosssymeter GL200 skin gloss tester to measure the glossiness of the contralateral arm, and use the skin gloss probe to test the selected part 3 times and record the average value. The larger the measured value, the higher the skin glossiness.

[0078] The test results are shown in Table 3-5.

[0079] Table 3 Statistics of skin roughness Stm

[0080]

[0081] Table 4 Statistics of skin roughness Sa

[0082]

[0083] Table 5 Statistics of skin glossiness

[0084]

[0085] It can be seen from the results of Tables 3-5 that skin care lotion sample No. 1 can significantly improve the roughness of the facial skin of the subject and increase the brightness of the facial skin. Skin care lotion samples No. 2 to No. 6 are significantly worse than No. 1 in improving the facial skin condition of the subject. This result shows that the combined effect of the four substances of ginsenoside Rh2, soybean polypeptide, β-sitosterol and calcium pantetheine sulfonate in the composition of the present invention has the effect of significantly improving skin brightness and reducing skin roughness.

[0086] Experiment 3: Evaluation of different skin care lotions on crow's feet, skin elasticity and skin firmness

[0087] The skin care lotion samples No. 1-6 prepared above were sent to a third-party testing agency for testing, and the crow's feet, skin elasticity and skin firmness of different skin care lotion samples were evaluated.

[0088] 1) T / CAB 0152-2022 Test methods for seven cosmetic efficacy items: anti-wrinkle, firming, moisturizing, oil control, repairing, nourishing, and soothing;

[0089] 2) T / TDCA 003-2021 Test method for firming efficacy of cosmetics;

[0090] 3) Liu Weiyi, Zhou Lin, Zhao Hua. Evaluation of cosmetic efficacy (ⅩⅢ) - Consumer use test[J]. China Surfactant Detergent & Chemical Industry, 2021, 51(06): 485-490;

[0091] 4) T / GDCA 029-2023 Evaluation of the efficacy of cosmetics for sensitive skin.

[0092] The specific method is: 30 healthy male / female subjects with sensitive skin, easy to turn red, loose skin around the eyes, lack of elasticity, obvious lines, fine lines or wrinkles, and wrinkles at the corners of the eyes, which meet the "SKIN AGING ATLAS" level 2 to 5, including 5 males and 25 females, with an age range of 25 to 60 years old. They were randomly divided into 6 groups, with 5 people in each group (1 male and 4 females). Skin care lotion samples No. 1-6 were used respectively. The skin elasticity and skin firmness of the subjects were measured by the method of before and after (D0 day and D28 day) control.

[0093] Skin elasticity R2 and skin firmness (R0, F4) were tested using the skin elasticity tester Cutometer dual MPA580; the test results are shown in Tables 6-8.

[0094] PRIMOS-CR Wrinkles at the corners of the eyes and wrinkles under the eyes (area, volume, number, length) were detected using the skin rapid optical imaging system PRIMOS-CR; the test results are shown in Tables 9-11.

[0095] Table 6 Skin elasticity R2 test results

[0096] serial number Base value (D0) Use the test product for 28 days (D28) Improvement rate after 28 days of using the test product (%) 1 0.52 0.61 17.31 2 0.51 0.56 9.80 3 0.52 0.58 11.54 4 0.51 0.57 11.76 5 0.5 0.55 10.00 6 0.51 0.53 3.92

[0097] Table 7 Skin firmness R0 test results

[0098] serial number Base value (D0) Use the test product for 28 days (D28) Improvement rate after 28 days of using the test product (%) 1 0.28 0.17 -39.29 2 0.28 0.22 -21.43 3 0.29 0.22 -24.14 4 0.27 0.2 -25.93 5 0.28 0.23 -17.86 6 0.27 0.25 -7.41

[0099] Table 8 Skin firmness F4 test results

[0100] serial number Base value (D0) Use the test product for 28 days (D28) Improvement rate after 28 days of using the test product (%) 1 6.53 4.98 -23.74 2 6.64 5.43 -18.22 3 6.61 5.29 -19.97 4 6.59 5.27 -20.03 5 6.51 5.33 -18.13 6 6.55 6.08 -7.18

[0101] Table 9 PRIMOS-CR eye wrinkle area detection results

[0102] serial number Base value (D0) Use the test product for 28 days (D28) Improvement rate after 28 days of using the test product (%) 1 41.51 36.02 -13.23 2 41.48 37.91 -8.61 3 41.5 37.18 -10.41 4 41.55 37.05 -10.83 5 41.46 37.98 -8.39 6 41.39 39.42 -4.76

[0103] Table 10 PRIMOS-CR eye wrinkle volume detection results

[0104] serial number Base value (D0) Use the test product for 28 days (D28) Improvement rate after 28 days of using the test product (%) 1 6.31 5.61 -11.09 2 6.85 6.35 -7.30 3 6.47 5.94 -8.19 4 6.62 6.05 -8.61 5 6.54 6.03 -7.80 6 6.69 6.36 -4.93

[0105] Table 11 PRIMOS-CR eye wrinkle number detection results

[0106] serial number Base value (D0) Use the test product for 28 days (D28) Improvement rate after 28 days of using the test product (%) 1 14.11 8.89 -37.00 2 13.67 10.01 -26.77 3 14.02 9.52 -32.10 4 13.89 9.23 -33.55 5 13.75 10.14 -26.25 6 13.82 11.59 -16.14

[0107] It can be seen from the results in Tables 6-11 that, compared with samples No. 2 to 6, continuous use of test product No. 1 for 28 days can effectively improve the volume, depth and area of ​​crow's feet, improve skin roughness, and enhance skin elasticity, firmness and glossiness.

[0108] In addition, during the trial period, the test products No. 1 to 6 were used continuously for 28 days. After safety evaluation by dermatologists, 0 adverse skin reactions were found among the 35 subjects, and 100.00% of the subjects felt that the products were mild and non-irritating, and 100.00% of the subjects felt that the products were suitable for sensitive skin. Therefore, it is believed that samples No. 1 to 6 are suitable for sensitive skin, mild and non-irritating.

[0109] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with this profession can make some changes or modifications to equivalent embodiments of equivalent changes by using the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A composition for increasing mitochondrial activity and activating cell energy, characterized in that: The composition comprises the following components in parts by weight: 0.0001-3 parts of citrulline, 0.0001-3 parts of sodium DNA, 0.0001-3 parts of taurine, 0.0001-2 parts of arginine / lysine polypeptide, 0.0001-2 parts of coenzyme Q10, 0.0001-2 parts of NAD+, 0.0001-0.5 parts of creatine, 0.0001-2 parts of adenosine, 0.0001-2 parts of ATP, 0.0001-2 parts of glutamine, 0.0001-1 parts of inositol, 0.0001-2 parts of carnosine, 0.0001-2 parts of glutathione, ginsenoside Rh2 0.0001-3 parts, soybean polypeptide 0.0001-3 parts, β-sitosterol 0.0001-2 parts and pantetheine sulfonic acid calcium 0.0001-1 parts.

2. A skin care product for improving mitochondrial activity and activating cell energy, characterized in that: The skin care product comprises the composition for improving mitochondrial activity and activating cell energy according to claim 1.

3. The skin care product for improving mitochondrial activity and activating cell energy according to claim 2, characterized in that: The skin care product comprises the following components in percentage by weight: 1-5% of a composition for improving mitochondrial activity and activating cell energy, 1-5% of a surfactant, 1-3% of a fatty acid, 2-6% of a phospholipid, 1-5% of an additive and the remainder of water.

4. The skin care product for improving mitochondrial activity and activating cell energy according to claim 3, characterized in that: The surfactant is selected from one or a combination of cetearyl glucoside, sorbitan olivate, polysorbate-80, polysorbate-60, ceteareth-2, polyglyceryl-10 laurate, polyoxyethylene castor oil, and polyoxyethylene sorbitan monooleate.

5. The skin care product for improving mitochondrial activity and activating cell energy according to claim 3, characterized in that: The fatty acid is selected from one or a combination of sunflower seed oil, avocado oil, meadowfoam seed oil, palmitic acid, linoleic acid, and linolenic acid.

6. The skin care product for improving mitochondrial activity and activating cell energy according to claim 3, characterized in that: The phospholipid is selected from one or a combination of lecithin, dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, and distearoylphosphatidylcholine.

7. The skin care product for improving mitochondrial activity and activating cell energy according to claim 3, characterized in that: The additives include 1,2-pentanediol, rose essential oil, disodium EDTA and glycerin.

8. The skin care product for improving mitochondrial activity and activating cell energy according to claim 7, characterized in that: The additive further comprises a pH regulator, and the pH regulator is selected from one or a combination of citric acid, malic acid, potassium hydroxide, arginine, and tromethamine.

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