Oligopeptide composition with repairing function and preparation method thereof

Abstract

The invention provides an oligopeptide repairing composition which can promote healing of human skin scratches, deeply repair skin barriers, improve skin sensitivity, solve the problems of dry skin, pruritus, redness and swelling, inflammation and the like, radically solve the problem of skin aging, effectively improve skin elasticity, fade fine wrinkles and make skin smooth and smooth. Therefore, the skin aging is delayed, and the health and beauty of the skin are guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of oligopeptides for promoting cell growth and proliferation, and in particular to an oligopeptide composition with a repairing function and a preparation method thereof. Background Art

[0002] Sensitive skin refers to a high-reaction state of the skin under physiological or pathological conditions, which is manifested by subjective symptoms such as burning, itching, stinging and tightness, accompanied or not accompanied by objective signs such as erythema, scaling and capillary dilation. People with allergies have sensitive skin, and applying seemingly simple cosmetics will also cause adverse reactions. Common ones include lipstick and hair dye, which will cause allergic problems such as redness, swelling, heat, itching, pain, and blisters after use. Not only can you not find the feeling of beauty, but embarrassment often occurs. In recent years, more and more people are troubled by sensitive skin problems. Studies have shown that the proportion of sensitive skin in women is 60%~70%, and that in men is 50%~60%. There are many factors that lead to skin sensitivity, such as oxidative stress caused by ultraviolet radiation, excessive loss of skin lipids caused by improper use of cleaning products, damage to the skin barrier function, air pollution, mental stress, etc., and even seasonal changes can cause skin sensitivity. Climate change, air pollution and the complexity of cosmetic types and ingredients have increased the possibility of sensitive skin appearing and receiving stimulation; with the improvement of people's awareness of skin health, the perception rate and attention of sensitive skin have gradually increased. Therefore, products that can quickly relieve subjective symptoms such as itching, tingling, burning, and objective symptoms such as redness and erythema are becoming more and more popular among consumers, and functional natural skin care products designed for people with sensitive skin are also gaining more and more attention. Inhibiting and resisting skin allergies is a very important issue from the perspective of skin physiology, medicine and beauty.

[0003] At present, it is generally believed that the occurrence of sensitive skin is a complex process involving skin barrier-neurovascular-immune inflammation. Therefore, the solution to skin sensitivity can be started from two main aspects, namely repairing the skin barrier and soothing the skin's inflammatory response. The skin barrier is mainly divided into three parts, consisting of keratinocytes, structural lipids, and hydrolipid membranes. The normal skin barrier is an important line of defense to protect the body, and has the functions of resisting the invasion of allergens, harmful substances, etc. and preventing water loss. Under the stimulation of internal and external factors such as age, ultraviolet rays, excessive cleaning, and wearing masks for a long time, skin barrier damage will be induced, affecting the normal repair function of the skin. Damage to the skin barrier is the basis for the formation of many common clinical skin diseases. Studies have shown that many skin-related problems are related to the skin barrier, such as sensitive skin, atopic dermatitis, and acne. Therefore, the repair of the skin barrier is an important part of the research and treatment of skin-related problems, and it is also the focus of product research and development by major cosmetics companies.

[0004] Cosmetics have become an indispensable part of our daily lives, especially among women. The cosmetics market, especially natural ingredient products, is growing rapidly, and consumers have a great demand for improved appearance. In particular, the development of new cosmetic formulations based on bioactive compounds such as antioxidants, proteins, peptides and growth factors has seen a rapid expansion in demand. Bioactive peptides have attracted great attention in the global cosmetics industry due to their potential in enhancing skin health and beauty. These small protein fragments have a variety of biological activities, such as antioxidant, anti-aging, anti-inflammatory and antibacterial activities, making them ideal ingredients for cosmetic formulations. Bioactive peptides participate in a variety of physiological and biochemical activities in the human body, and their biological activity is extremely high. A small dose of peptides can play a biological regulatory role in a variety of cellular physiological functions and metabolic activities. The repair effect of peptides on the above skin problems is obvious, and it is one of the most recognized and safe active raw materials for skin repair.

[0005] In the prior art, various peptides have been reported to improve skin function. Their individual peptides or peptide combination products can be used in skin improvement products. Patent CN202210542473.9 discloses a peptide composition for anti-inflammatory, repair and allergy-relief with the addition of paeoniflorin and vanillin methyl ether. The addition of paeoniflorin and vanillin methyl ether to the composition can further enhance the skin barrier repair performance of the anti-inflammatory, allergy-relief and allergy-relief peptide composition, achieving a synergistic enhancement effect. Patent CN202011236017.9 discloses a multi-group peptide compound containing acetyl heptapeptide-4 and the like for soothing, anti-allergy and skin repair.

[0006] It is known that heptapeptide-6 is a polypeptide molecule with strong biological activity. Heptapeptide-6 is usually added to skin care products for anti-wrinkle and anti-aging effects. However, technicians have found that it is a powerful growth factor; and studies have shown that heptapeptide-6 can promote cell growth and proliferation, especially the growth of skin cells. Heptapeptide-6 also has antibacterial and anti-inflammatory effects. Studies have found that heptapeptide-6 can form a complex with lipopolysaccharide on the bacterial membrane to destroy the integrity of the bacterial membrane and inhibit bacterial growth. At the same time, heptapeptide-6 can also reduce inflammatory reactions and improve the healing rate of wounds.

[0007] However, the skin barrier repair effect of single peptides or polypeptide combinations reported in the prior art is not ideal, especially for severely damaged skin barrier and skin damage. Providing more safe products that can deeply repair the skin barrier and improve allergic skin is a technical problem that technicians in this field urgently need to solve. Summary of the invention

[0008] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide an oligopeptide repair composition, which can promote the healing of human skin scratches, deeply repair the skin barrier, improve skin sensitivity, and has good antibacterial and anti-inflammatory effects.

[0009] Specifically, the present invention is achieved through the following technologies: An oligopeptide repair composition comprises an oligopeptide for promoting cell growth and proliferation and a synergistic component; the oligopeptide for promoting cell growth and proliferation is heptapeptide-6 with an amino acid sequence of Gly-Tyr-Tyr-Glu-Leu-Asn-Asp.

[0010] Preferably, the first synergistic component is a deoxyribonucleotide polymer PDRN.

[0011] Preferably, the second synergistic ingredient is a short peptide, more preferably one or more of dipeptide-1, palmitoyl hexapeptide-12, tripeptide-2, tripeptide-10 citrulline, and carnosine.

[0012] Preferably, the content of oligopeptide heptapeptide-6 that promotes cell growth and proliferation in the composition is 0.5-3wt%; more preferably 1.0-2.0wt%.

[0013] Preferably, the content of the first synergistic component deoxyribonucleotide polymer PDRN in the composition is 0.5-2.0wt%; more preferably 0.8-1.5wt%.

[0014] Preferably, the content of the second synergistic ingredient short peptide in the composition is 0.01-0.5wt%; more preferably 0.1-0.5wt%.

[0015] In a preferred embodiment, the oligopeptide repair composition comprises the following components in parts by weight: 0.5-3wt% of heptapeptide-6, 0.5-2.0wt% of deoxyribonucleotide polymer PDRN, 0.01-0.5wt% of dipeptide-1, 0.01-0.5wt% of palmitoyl hexapeptide-12, 0.01-0.5wt% of tripeptide-2, 0.01-0.5wt% of tripeptide-10 citrulline, and 0.01-0.5wt% of carnosine.

[0016] In a preferred embodiment, the oligopeptide repair composition comprises the following components in parts by weight: 1.0-2.0 wt% of heptapeptide-6, 0.8-1.5 wt% of deoxyribonucleotide polymer PDRN, 0.1-0.5 wt% of dipeptide-1, 0.1-0.5 wt% of palmitoyl hexapeptide-12, 0.1-0.5 wt% of tripeptide-2, 0.1-0.5 wt% of tripeptide-10 citrulline, and 0.1-0.5 wt% of carnosine.

[0017] In a preferred embodiment, the oligopeptide repair composition comprises the following components in parts by weight: 1.5wt% of heptapeptide-6, 1.0wt% of deoxyribonucleotide polymer PDRN, 0.4wt% of dipeptide-1, 0.3wt% of palmitoyl hexapeptide-12, 0.2wt% of tripeptide-2, 0.3wt% of tripeptide-10 citrulline, and 0.5wt% of carnosine.

[0018] The oligopeptide repair composition provided by the present invention can be prepared into lyophilized powder by freeze-drying, and can be used directly after being dissolved in water; it can also be further compounded with other preparation raw materials, and auxiliary ingredients can be added according to the product dosage form and usage requirements to prepare skin care products in various dosage forms such as essence, lyophilized powder, cream, gel, facial mask, and skin lotion.

[0019] Preferably, the oligopeptide repair composition further comprises auxiliary ingredients, and the auxiliary ingredients include moisturizers, solubilizers, antibacterial agents, thickeners, pH balance agents, and aqueous solvents.

[0020] The moisturizer is preferably one or more of glycerol, propylene glycol, hyaluronic acid, and butylene glycol, and is more preferably a mixture of glycerol and butylene glycol; its content is selected according to demand and common knowledge in the art.

[0021] The solubilizer is preferably one or more of PEG hydrogenated castor oil, fatty alcohol polyoxyethylene ether, polyoxyethylene sorbitan fatty acid ester, and polyglycerol fatty acid ester.

[0022] The antibacterial agent is preferably one or more of phenoxyethanol, silver ions, parabens, benzoic acid, phenol, PHL, scutellaria baicalensis extract, phellodendron amurense extract, forsythia suspensa extract, dandelion extract and aloe extract; the content thereof is selected according to demand and conventional knowledge in the art, and the antibacterial agent is used at a concentration that is harmless and non-irritating to the human body while achieving a good antibacterial effect.

[0023] The thickener is selected from one or more of alginic acid, starch, gum arabic, pectin, agar, gelatin, algin, carrageenan, dextrin, triethanolamine, sodium carboxymethyl cellulose, methyl cellulose, sodium starch phosphate, sodium polyacrylate, polyoxyethylene, polyvinyl pyrrolidone, bomer, xanthan gum, triethanolamine, HEC (hydroxyethyl cellulose); more preferably, it is a starch-colloid compound; and particularly preferably, it is a starch-xanthan gum compound with a mass ratio of 1:2~3.

[0024] The pH balance agent is selected from one or more of triethanolamine, lactate, hyaluronic acid and acetic acid.

[0025] The present invention further provides an oligopeptide repair composition, wherein the contents of the components in the composition are as follows: Among them, the pH value range of the oligopeptide repair composition is 4~6.

[0026] In a preferred embodiment, the contents of the components in the composition of the present invention are as follows: Among them, the pH value range of the oligopeptide repair composition is 5~6.

[0027] In one specific embodiment of the technical solution of the present invention, the contents of the components in the composition are as follows: Among them, the pH value range of the oligopeptide repair composition is 5.5. On the other hand, the present invention provides a method for preparing an oligopeptide repair composition, comprising adding a prescribed amount of heptapeptide-6, PDRN, a water-soluble short peptide, a starch-xanthan gum compound, and an appropriate amount of purified water into a container, stirring at room temperature to dissolve, and preparing an aqueous phase; adding a fat-soluble short peptide, glycerol, butanediol, and PEG hydrogenated castor oil into the container to dissolve, and preparing an oil phase; adding the aqueous phase into the oil phase, stirring evenly, adding PHL, adjusting the pH value to 4-6 with acid and alkali (acetic acid and triethanolamine), adding water to the full amount, filtering, and packaging to obtain the target product.

[0028] Beneficial effects of the present invention: The present application provides a peptide-containing composition for multiple skin barrier repairs. Under the synergistic effect of the deoxyribonucleotide polymer PDRN and the short peptide, the heptapeptide-6 can efficiently and deeply repair damaged skin, thereby achieving the effect of repairing skin damage at the cellular level, while improving the skin microecological barrier, improving allergic skin, and solving problems such as dry skin, itching, redness, swelling, and inflammation. It solves the problem of skin aging from the root, and can effectively improve skin elasticity, fade fine lines, and then delay skin aging, thereby ensuring the health and beauty of the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 : In the cell scratch repair test, the cell morphology of the negative control group -0h and the negative control group, the sample of comparative example 1, and the sample of embodiment 1 was observed under a microscope after 24h of culture. DETAILED DESCRIPTION

[0030] The present invention is further described below by way of examples. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are reagents and materials that can be obtained from commercial sources unless otherwise specified.

[0031] Example 1 Preparation of 1 kg of serum According to the prescription in Table 1, heptapeptide-6, PDRN, water-soluble short peptides, starch-xanthan gum compound, and an appropriate amount of purified water were added to a container and stirred at room temperature to dissolve to prepare an aqueous phase; fat-soluble short peptides, glycerol, butylene glycol, and PEG hydrogenated castor oil were dissolved to prepare an oil phase; the aqueous phase and the oil phase were mixed, PHL was added, the pH value was adjusted to 4-6 with acid and alkali (acetic acid and triethanolamine), and water was added to the full amount.

[0032] Table 1

[0033] The specific preparation process is as follows: Step 1: Add the prescribed amount of heptapeptide-6, PDRN, dipeptide-1, palmitoyl hexapeptide-12, tripeptide-10 citrulline, carnosine, starch-xanthan gum compound, and water into a container and stir at room temperature to dissolve to prepare an aqueous phase; Step 2: Add tripeptide-2, glycerin, butylene glycol, and PEG hydrogenated castor oil into a container and heat to 60°C and stir until dissolved to prepare the oil phase; Step 3: Add the water phase to the oil phase, stir well, add PHL, and adjust the pH to 5.5 with acid and base (acetic acid and triethanolamine).

[0034] Step 4: Add water to the full amount, filter, and package.

[0035] Embodiment 2-8

[0036] Example 2-8 Refer to the prescription in Table 2 to prepare 1 kg of essence.

[0037] Table 2

[0038] Examples 2-8 refer to the preparation scheme of the examples and prepare essence products according to the prescription amount in Table 2.

[0039] Comparative Examples 1-7

[0040] The components of the compositions of Comparative Examples 1-7 are shown in Table 3, and they were prepared by referring to the method of Example 1.

[0041] Table 3

[0042] The difference from Example 1 is that: the first synergistic ingredient PDRN is not added to the components of Comparative Example 1; the second synergistic ingredient in the components of Comparative Example 2 does not include carnosine; the auxiliary component thickener in the components of Comparative Example 3 replaces the starch-xanthan gum compound with xanthan gum; the second synergistic ingredient in the components of Comparative Example 4 replaces palmitoyl hexapeptide-12 with palmitoyl hexapeptide-6; the second synergistic ingredient in the components of Comparative Example 5 does not include tripeptide-2 and tripeptide-10 citrulline, and oligopeptide-1 and oligopeptide-3 are added; the second synergistic ingredient in the components of Comparative Example 6 replaces dipeptide-1 with dipeptide-2; the second synergistic ingredient in the components of Comparative Example 7 does not include dipeptide-1, palmitoyl hexapeptide-12, tripeptide-2, and tripeptide-10 citrulline.

[0043] Comparative Example 8

[0044] 100g of polypeptide composition was prepared according to the following prescription: 0.02% heptapeptide-6, 0.01% oligopeptide-1, 0.02% oligopeptide-3, 0.01% yeast polypeptides, 0.4% carnosine, 0.02% cono peptide, 0.02% nonapeptide-1, 0.1% glutathione, 0.02% acetyl octapeptide-3, 0.05% sodium hyaluronate, 6% mannitol, 1% trehalose, and appropriate amount of water. The specific implementation plan is: sodium hyaluronate, mannitol, and trehalose are dissolved and sterilized with appropriate amount of water to prepare a sterile base; the prescribed amount of heptapeptide-6, oligopeptide-1, oligopeptide-3, yeast polypeptides, carnosine, cono peptide nonapeptide-1, glutathione, and acetyl octapeptide-3 are dissolved in water, sterilized and filtered, mixed with the sterile base, and water is added to the full amount.

[0045] Verification example: Animal testing to improve skin repair

[0046] The mouse AD model was established by repeated stimulation with DNCB hapten. The hair on the back of the mouse neck was cut with scissors one day before the experiment, and then the hair was removed with depilatory cream to expose the skin. The depilatory area was about 3cm×3cm. On the first day of the experiment, 0.5% DNCB was applied to the shaved skin of the mouse back (100 µl) for initial sensitization. On the 5th, 7th, 9th, 11th, and 13th days, 0.25% DNCB was applied to the shaved skin of the mouse back (100 µl) for stimulation, that is, stimulation once every other day for 10 days. The control group was only applied with DNCB solvent, and the application time and application dose were the same as those of the model group. After the model was established, the mice in the experimental group showed strong spontaneous scratching, and the skin area showed reduced hydration of the stratum corneum, formation of epidermal serous crusts in the skin tissue, and mild hyperplasia.

[0047] The control group during modeling was used as the blank control group, and several mice with successful modeling were randomly divided into a model control group and a model administration group. There were 6 mice in each group, half of them were male and half were female. Take 2 ml of the composition prepared in Examples 1-8 of the present invention and Comparative Examples 1-8 and apply it on the skin of mice in the model administration group once a day; give the sample for 15 days. The blank control group and the model control group were smeared with an equal amount of distilled water. After 15 days of administration, the number of scratching times of the mice within 3 minutes was observed and the antipruritic inhibition rate was calculated. The antipruritic inhibition rate (%) = (model control group-model administration group) / (model control group-blank control group)*100%. The transepidermal water loss rate was determined using a transepidermal water loss tester. One day after the last sample was given, the mice were killed by dislocating the neck, and the back skin was punched to measure the skin thickness.

[0048] Table 4 Experimental results of mouse skin repair effect

[0049] Note: Compared with the model control group, *P<0.05.

[0050] The above results show that the composition of the present invention has an excellent skin barrier repair effect under the synergistic effect of the deoxyribonucleotide polymer and the short peptide, can effectively alleviate the skin lesions on the back of mice, effectively solve the problem of skin itching, and significantly reduce the transepidermal water loss rate of the skin. The composition of the present invention has good soothing properties; at the same time, it can also effectively reduce the phenomenon of epidermal hyperplasia.

[0051] Cell scratch repair assay Human HaCaT cells were taken and digested with 0.25% trypsin. MEM containing 15% FBS was used as the culture medium. The bottom of the 2-well scratch insert was placed on a 6-well plate using sterile tweezers. The density of the keratinocyte suspension was adjusted to 4.0×10 5 70 μL per well was inoculated into a 6-well plate and incubated overnight in a biochemical incubator at 37°C and 5% CO2.

[0052] After the plug was removed, the supernatant was discarded, and the complete medium containing no serum was used as the diluent, and 2 mL / well of the samples of Example 1 and Comparative Examples 1-8 and the negative control group (MEM complete medium) were added into the wells. After culturing in a 37°C, 5% CO2 incubator for 24 h, the cell morphology was observed under a microscope and photographed.

[0053] Image J software was used to perform statistical area analysis on the images, and the scratch healing rate was calculated as follows: ;

[0054] Table 5 Cell scratch area and healing rate

[0055] According to the above results, the relative healing rate of scratches in the composition of the present invention reaches 74.54% under the synergistic effect of the deoxyribonucleotide polymer and the short peptide, and it has a good function of promoting the repair of damaged skin.

[0056] Lactic acid sting test score Thirty female subjects aged 18 to 45 years who were positive in the lactic acid sting test were selected and randomly divided into two groups. Exclusion criteria: women who are currently pregnant or breastfeeding; those who have used antihistamines in the past week or immunosuppressants in the past month; those who have applied any anti-inflammatory drugs to the test site in the past 2 months; subjects with inflammatory skin diseases that have not been cured clinically; patients with insulin-dependent diabetes or asthma or other chronic respiratory diseases being treated; patients with immunodeficiency or autoimmune diseases; those whose test results are affected by scars, pigments, atrophy, port wine stains or other blemishes on the skin to be tested; those with highly sensitive constitutions; subjects who have participated in related or other clinical trials in the past month and other activities that may affect the test results.

[0057] This study adopted a before-after control method. The subjects used it every morning and evening after cleansing their faces. The first group took an appropriate amount of the sample of Example 1 of the present invention, and the second group took an appropriate amount of the sample of Comparative Example 8, and applied it to the entire face in a circular manner until absorbed, twice a day, for 28 consecutive days. Clinical testing and evaluation were carried out on day 0 and day 28, respectively.

[0058] Lactic acid stinging value scoring: The subjects cleaned their faces and wiped them dry with lint-free paper. After sitting quietly for 30 minutes in a constant temperature (20-22)℃ and constant humidity 50%±5%, 50μL of 10% lactic acid aqueous solution was applied to the cheek on either side of the nasolabial groove. The subjects evaluated the discomfort of itching, stinging, and burning pain at the test site at 30 seconds, 2.5 minutes, and 5 minutes. The score was scored according to the 4-point method (0 points for no feeling, 1 point for mild, 2 points for moderate, and 3 points for severe). The cumulative stinging sensation of 30 seconds, 2.5 minutes, and 5 minutes was greater than or equal to 3 points, which was considered positive for lactic acid stinging.

[0059] Table 6 Skin lactic acid stinging score

[0060] In this experiment, the lactic acid stinging values ​​at the time of entry and exit were statistically analyzed, and the skin lactic acid stinging score values ​​are shown in Table 6. According to the experimental data records, by using the samples of the present invention, the test subjects' skin response to external stimuli was significantly reduced, which shows that the product of the present invention has a significant effect on repairing the skin barrier.

[0061] It is explained here that the embodiments of the present invention are only used to illustrate the present invention, rather than to limit the present invention. Therefore, simple improvements to the present invention based on the method of the present invention are all within the scope of protection required by the present invention.

Claims

1. An oligopeptide repair composition, characterized in that: The composition comprises an oligopeptide for promoting cell growth and proliferation, a synergistic component and necessary auxiliary components; the oligopeptide for promoting cell growth and proliferation is heptapeptide-6 with an amino acid sequence of Gly-Tyr-Tyr-Glu-Leu-Asn-Asp.

2. The oligopeptide repair composition according to claim 1, characterized in that: The synergistic component of the composition is a deoxyribonucleotide polymer PDRN.

3. The oligopeptide repair composition according to claim 1, characterized in that: The synergistic component of the composition is a short peptide, and the short peptide is selected from one or more of dipeptide-1, palmitoyl hexapeptide-12, tripeptide-2, tripeptide-10 citrulline, and carnosine.

4. The oligopeptide repair composition according to claim 3, characterized in that: The content of oligopeptide heptapeptide-6 for promoting cell growth and proliferation in the composition is 0.5-3wt%, the content of synergistic component deoxyribonucleotide polymer PDRN in the composition is 0.5-2.0wt%, and the content of synergistic component short peptide in the composition is 0.01-0.5wt%.

5. The oligopeptide repair composition according to claim 3, characterized in that: The composition comprises the following components in parts by weight: 0.5-3.0 wt% of heptapeptide-6, 0.5-2.0 wt% of deoxyribonucleotide polymer PDRN, 0.01-0.5 wt% of dipeptide-1, 0.01-0.5 wt% of palmitoyl hexapeptide-12, 0.01-0.5 wt% of tripeptide-2, 0.01-0.5 wt% of tripeptide-10 citrulline, and 0.01-0.5 wt% of carnosine.

6. The oligopeptide repair composition according to claim 3, characterized in that: The composition comprises the following components in parts by weight: 1.0-2.0 wt% of heptapeptide-6, 0.8-1.5 wt% of deoxyribonucleotide polymer PDRN, 0.1-0.5 wt% of dipeptide-1, 0.1-0.5 wt% of palmitoyl hexapeptide-12, 0.1-0.5 wt% of tripeptide-2, 0.1-0.5 wt% of tripeptide-10 citrulline, and 0.1-0.5 wt% of carnosine.

7. The oligopeptide repair composition according to claim 3, characterized in that: The composition includes the following components in parts by weight: 1.5wt% heptapeptide-6, 1.0wt% deoxyribonucleotide polymer PDRN, 0.4wt% dipeptide-1, 0.3wt% palmitoyl hexapeptide-12, 0.2wt% tripeptide-2, 0.3wt% tripeptide-10 citrulline, and 0.5wt% carnosine.

8. The oligopeptide repair composition according to claim 1, characterized in that: The auxiliary components in the composition include a moisturizer, a solubilizer, an antibacterial agent, a thickener, a pH balance agent, and a water solvent.

9. The oligopeptide repair composition according to claim 9, characterized in that: The content of the components in the composition is as follows: Among them, the pH value range of the oligopeptide repair composition is 4~6.

10. The oligopeptide repair composition according to any one of claims 1 to 9, characterized in that: The composition can be prepared into lyophilized powder by freeze-drying, and can be used directly after being dissolved in water; it can also be further compounded with other preparation raw materials, and auxiliary ingredients can be added according to product dosage forms and usage requirements to prepare skin care products in various dosage forms such as essences, lyophilized powders, creams, gels, facial masks, and skin lotions.

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