Functional microbial substrate amendment and its application in shrimp and crab culture

Abstract

The present application belongs to the field of aquaculture, and particularly relates to a functional microbial substrate modifier and its application in shrimp and crab culture, which comprises activated Yarrowia lipolytica fermented sludge powder and adsorbent composite powder, microbial inoculum and mineral additive. The functional microbial substrate modifier is in granular form, and contains various microorganisms, minerals and nutrients required by shrimps and crabs. When applied in shrimp and crab culture, the functional microbial substrate modifier can improve the body color of the cultured shrimps and crabs, increase the immunity and activity, and make the body color healthier. The shrimps and crabs can better cope with extreme weather and water environment, and have strong stress resistance. The functional microbial substrate modifier also promotes the feeding of shrimps and crabs, and can increase the weight of shrimps and crabs to a certain extent.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and in particular to a functional microbial substrate improver and application thereof in shrimp and crab farming. Background Art

[0002] Water quality improvers refer to the addition of physically or chemically active drugs to reduce the amount of harmful substances in aquaculture water that affect the growth and reproduction of fish, such as ammonia nitrogen, nitrite, sulfide, suspended matter, etc., thereby making the water more suitable for the needs of aquaculture.

[0003] Existing substrate improvers can be divided into many types, including physical adsorption type, chemical type, biological type, etc.

[0004] Physical adsorption agents, such as zeolite powder, medical stone, and activated carbon, primarily remove harmful substances such as ammonia nitrogen, nitrite, and organic matter from water bodies through physical adsorption. The advantage of this type of amendment is that it quickly adsorbs and reduces harmful substances in water bodies. However, the disadvantage is that long-term use may cause substrate deterioration and lacks degradation capabilities.

[0005] Chemical types include quaternary phosphates and strong oxidants. Quaternary phosphates break down surface tension on the pond bottom, degrade organic matter, and inhibit and kill bacteria. However, the presence of phosphorus can easily lead to algae blooms, and their effectiveness is significantly affected by water temperature. Strong oxidants, such as potassium persulfate, improve dissolved oxygen levels by oxidizing organic matter, but their mildness and irritation should be considered.

[0006] Bio-based agents primarily consist of microbial agents, such as Bacillus subtilis and photosynthetic bacteria. These agents decompose organic matter through microbial metabolism, reducing ammonia nitrogen and nitrite concentrations and restoring the microbial ecosystem of the water body. These agents are highly safe and have no side effects, but they are slow to take effect and are significantly affected by water temperature and pesticide residues.

[0007] Detoxification type: such as sodium humate, EDTA, etc., mainly used to complex and transform toxic substances to reduce their toxicity or make them non-toxic.

[0008] For some companies engaged in microbial fermentation, their fermentation sludge generally needs to be treated harmlessly, and its secondary utilization has always been a problem.

[0009] Microbial fermentation sludge contains residual nutrients required for microbial growth. For biological substrate improvers, if the two can be combined, while also solving the defect of slow effect, it is a difficult problem that needs to be solved urgently.

[0010] Based on this, the present invention is proposed. Summary of the Invention

[0011] The purpose of the present invention is to provide a functional microbial substrate improver and its application in shrimp and crab farming to solve the above problems.

[0012] The purpose of the present invention is to provide a functional microbial substrate improver and its application in shrimp and crab farming, which can be achieved through the following technical solutions:

[0013] Functional microbial substrate improver, including the following components:

[0014] The activated Yarrowia lipolytica fermentation sludge powder is combined with adsorbent composite powder, microbial agent and mineral additive.

[0015] Furthermore, the mass ratio of the activated Yarrowia lipolytica fermentation sludge powder to the adsorbent composite powder and the mineral additive is 100:(5-10).

[0016] Furthermore, the Yarrowia lipolytica fermentation sludge reacts in a liquid battery through a liquid-electric effect to obtain activated Yarrowia lipolytica fermentation sludge powder. The production method of the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder includes the following steps:

[0017] Step 1: feeding the fermentation sludge of Yarrowia lipolytica into a liquid cell, adding water and an adsorbent into the liquid cell and stirring until a thin sludge is obtained, wherein the solid content of the thin sludge is 35% to 38%, the height of the thin sludge does not exceed half the height of the liquid cell, and the mass ratio of the fermentation sludge of Yarrowia lipolytica to the adsorbent is 100:17;

[0018] Step 2: Cover the battery with a cell cover. The cell cover is provided with multiple air holes. The ratio of the total area of ​​all air holes to the volume of the battery is 15-20 cm 2 / m 3 ; The power source connected to the electrodes of the liquid battery is pulse electricity, the voltage of the pulse electricity is 10kV, and the pulse frequency is 10-25Hz; after 11-12 minutes of liquid-electric treatment, the liquid-electric treatment is stopped, the pool cover is opened, and the material in the liquid battery is sucked into the filter press for filtration. The obtained filter cake is dried, crushed, and sieved to obtain a powder with a particle size of less than or equal to 500μm, which is the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder.

[0019] Furthermore, the microbial agent is one or more of Bacillus, nitrifying bacteria, denitrifying bacteria, lactic acid bacteria, photosynthetic bacteria, and Clostridium butyricum.

[0020] Furthermore, the content of live bacteria in the functional microbial substrate improver is 500 million to 3 billion per gram.

[0021] Furthermore, the mineral additive is composed of major element minerals and trace element minerals, the major elements contained in the major element minerals are one or more of calcium, phosphorus, magnesium, sulfur, sodium, potassium, and chlorine, and the trace elements contained in the trace element minerals are one or more of iron, copper, manganese, zinc, selenium, iodine, and cobalt.

[0022] Furthermore, the adsorbent is a molecular sieve.

[0023] Furthermore, the preparation method of the functional microbial substrate improver is as follows: the activated Yarrowia lipolytica fermentation sludge powder and the adsorbent composite powder are moistened with water to obtain a moistened mud material, wherein the amount of water added is 2.2% to 2.5% of the mass of the activated Yarrowia lipolytica fermentation sludge powder and the adsorbent composite powder; the moistened mud material is mixed and stirred evenly with microbial agents and mineral additives, and then dried, crushed, and sieved to obtain a granular functional microbial substrate improver.

[0024] Furthermore, the functional microbial substrate improver is used in shrimp and crab farming.

[0025] Furthermore, the functional microbial substrate improver is pre-buried in the bottom mud of the shrimp and crab farms by pre-buried method, and the dosage of the functional microbial substrate improver is 41±0.3kg / hm 2 .

[0026] Phaffia rhodozyma is an obligate aerobic bacterium, and its fermentation products mainly include carotenoids, proteins, fats and carbohydrates.

[0027] When a pulsed high voltage is applied to a pair of electrodes placed in a liquid, the gap between the electrodes is instantly broken down, generating a strong arc spark discharge, accompanied by a series of significant physical, chemical, biological, and mechanical effects. This process is called the hydrostatic phenomenon / hydrostatic effect.

[0028] When the hydroelectric effect occurs, a mechanical effect (mechanical effect) is also generated. The radially propagating mechanical pressure wave produces a strong mechanical action. During liquid-phase discharge, the combined effects of acoustic, optical, thermal, and shock waves lead to significant changes in the chemical composition of the liquid phase, exemplified by the emergence of various free radicals and their products, making the liquid extremely chemically active. During liquid-phase discharge, strong current pulses appear in the discharge channel, creating a strong pulsed magnetic field around the discharge channel, subjecting the surrounding environment to the influence of a strong electromagnetic field. The high temperature and high pressure generated by liquid-phase discharge cause localized liquid in the liquid phase to enter a supercritical state, resulting in significant changes in the liquid's properties, imbuing this portion with supercritical fluid properties. The biological effects of liquid-phase discharge can be attributed to the phenomenon in which the combined effects of multiple mechanical, acoustic, optical, electromagnetic, and chemical effects on organisms within the liquid cause significant changes in their activity, viability, and other dynamics. This can include sterilization and microbial inactivation.

[0029] For Yarrowia lipolytica fermentation sludge, if there are no pores on the pool cover, the material in the final liquid cell will be very fine and will easily become hardened immediately after subsequent water absorption and wetting, which is not conducive to the production of granules. If there are too many pores on the pool cover, for example, the ratio of the total area of ​​all pores to the volume of the liquid cell is 35cm 2 / m 3 , which will lead to excessive oxygen participation when the electrohydraulic effect occurs, and eventually some components in the Yarrowia lipolytica fermentation sludge will be oxidized in large quantities, resulting in the structure being no longer loose, and the specific surface area of ​​the composite powder does not exceed 100m 2 / g; and the specific surface area of ​​the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder of the present invention exceeds 387m 2 / g.

[0030] Bacillus: Possessing powerful decomposition capabilities, it can rapidly degrade organic matter and harmful substances, improving water quality. Bacillus can also grow effectively in low-oxygen conditions and is suitable for anaerobic environments.

[0031] Nitrifying bacteria: These bacteria can convert ammonia nitrogen into nitrates, thereby reducing the content of harmful substances in water bodies.

[0032] Denitrifying bacteria: In an anoxic environment, denitrifying bacteria can reduce nitrates to nitrogen gas, thereby removing nitrogen pollution from water bodies.

[0033] Lactic acid bacteria: Lactic acid bacteria decompose organic matter through anaerobic fermentation, producing beneficial substances such as lactic acid, which helps to improve the bottom environment.

[0034] Photosynthetic bacteria: Photosynthetic bacteria can use light energy to carry out photosynthesis in a weak light environment, decompose organic matter and increase dissolved oxygen content.

[0035] Clostridium butyricum: This strain of bacteria grows well in anaerobic environments, can absorb harmful substances such as hydrogen sulfide at the bottom of the pond, and produce lactic acid and biological enzymes, creating a superior growth environment for farmed animals.

[0036] Through synergistic action, these microbial strains can effectively decompose pollutants such as organic leftover bait and feces in the bottom mud, converting them into nutrients that can be absorbed by algae and aquatic plants, while inhibiting the growth of harmful bacteria, thereby improving water quality and promoting the healthy growth of shrimp and crabs.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] 1. The functional microbial substrate improver of the present invention is in granular form and contains a variety of microorganisms, multiple minerals and nutrients required by shrimps and crabs. When used in shrimp and crab farming, it can improve the body color of farmed shrimps and crabs, enhance immunity and vitality, and make the body color healthier; shrimps and crabs can also better cope with extreme weather and water environments and have strong anti-stress capabilities; it also has the function of promoting shrimps and crabs to attract food and can increase the weight of shrimps and crabs to a certain extent.

[0039] 2. It can also increase the redox potential of the water body and the bottom, oxidize the black and smelly bottom mud, improve the bottom deodorization; effectively remove organic pollutants at the bottom of the pond, reduce chemical oxygen demand, effectively remove inorganic pollutants at the bottom of the pond, remove ammonia nitrogen, nitrite, hydrogen sulfide, etc., and prevent shrimp and crab poisoning and death. It can also effectively remove algae toxins and effectively reduce shrimp and crab mortality.

[0040] 3. The fermentation sludge of Yarrowia lipolytica is treated in a liquid cell to be effectively activated, making it more suitable as a nutrient matrix for a substrate improver. At the same time, it is also beneficial to fuse the various components of the substrate improver into granules, with a high yield rate, and suitable for industrial production. DETAILED DESCRIPTION

[0041] The present invention is further described in detail below through specific examples.

[0042] The following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present application without creative work are within the scope of protection of the present application.

[0043] Example 1

[0044] Functional microbial substrate improver, including the following components:

[0045] The activated Yarrowia lipolytica fermentation sludge powder, adsorbent composite powder, microbial agent and mineral additive are mixed in a mass ratio of 100:5.

[0046] The microbial agent is a composite of Bacillus, nitrifying bacteria, denitrifying bacteria, lactic acid bacteria, photosynthetic bacteria and Clostridium butyricum. The content of live bacteria in the functional microbial substrate improver is 500 million to 3 billion per gram.

[0047] The Yarrowia lipolytica fermentation sludge reacts in a liquid cell through a liquid-electric effect to obtain activated Yarrowia lipolytica fermentation sludge powder. The production method of the activated Yarrowia lipolytica fermentation sludge powder and an adsorbent composite powder comprises the following steps:

[0048] Step 1: feeding the fermentation sludge of Yarrowia lipolytica into a liquid cell, adding water and an adsorbent into the liquid cell and stirring until a thin sludge is obtained, wherein the solid content of the thin sludge is 38%, the height of the thin sludge does not exceed half the height of the liquid cell, and the mass ratio of the fermentation sludge of Yarrowia lipolytica to the adsorbent is 100:17;

[0049] Step 2: Cover the liquid battery with a cell cover. The cell cover is provided with multiple air holes. The ratio of the total area of ​​all air holes to the volume of the liquid battery is 15cm 2 / m 3 ; The power source connected to the electrodes of the liquid battery is pulse electricity, the voltage of the pulse electricity is 10kV, and the pulse frequency is 10Hz; after 11 minutes of liquid-electric treatment, the liquid-electric treatment is stopped, the pool cover is opened, and the material in the liquid battery is sucked into the filter press for filtration. The obtained filter cake is dried, crushed, and sieved to obtain a powder with a particle size less than or equal to 500μm, which is the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder.

[0050] The mineral additives are composed of major element minerals and trace element minerals. The major elements contained in the major element minerals are one or more of calcium, phosphorus, magnesium, sulfur, sodium, potassium, and chlorine. The trace elements contained in the trace element minerals are one or more of iron, copper, manganese, zinc, selenium, iodine, and cobalt.

[0051] The adsorbent is a molecular sieve.

[0052] The activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder are moistened with water to obtain a moistened mud material, wherein the amount of water added is 2.2% of the mass of the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder; the moistened mud material is mixed and stirred evenly with a microbial agent and a mineral additive, and then dried in the sun to obtain a dry block; the dry block is broken and sieved to obtain a granular functional microbial substrate improver.

[0053] Example 2

[0054] Functional microbial substrate improver, including the following components:

[0055] The activated Yarrowia lipolytica fermentation sludge powder, adsorbent composite powder, microbial agent and mineral additive are mixed in a mass ratio of 100:10.

[0056] The microbial agent is a composite of Bacillus, nitrifying bacteria, denitrifying bacteria, lactic acid bacteria, photosynthetic bacteria and Clostridium butyricum. The content of live bacteria in the functional microbial substrate improver is 500 million to 3 billion per gram.

[0057] The Yarrowia lipolytica fermentation sludge reacts in a liquid cell through a liquid-electric effect to obtain activated Yarrowia lipolytica fermentation sludge powder. The production method of the activated Yarrowia lipolytica fermentation sludge powder and an adsorbent composite powder comprises the following steps:

[0058] Step 1: feeding the fermentation sludge of Yarrowia lipolytica into a liquid cell, adding water and an adsorbent into the liquid cell and stirring until a thin sludge is obtained, wherein the solid content of the thin sludge is 35%, the height of the thin sludge does not exceed half the height of the liquid cell, and the mass ratio of the fermentation sludge of Yarrowia lipolytica to the adsorbent is 100:17;

[0059] Step 2: Cover the liquid battery with a cell cover. The cell cover is provided with multiple air holes. The ratio of the total area of ​​all air holes to the volume of the liquid battery is 20cm 2 / m 3 ; The power source connected to the electrodes of the liquid battery is pulse electricity, the voltage of the pulse electricity is 10kV, and the pulse frequency is 25Hz; after 12 minutes of liquid-electric treatment, the liquid-electric treatment is stopped, the pool cover is opened, and the material in the liquid battery is sucked into the filter press for filtration. The obtained filter cake is dried, crushed, and sieved to obtain a powder with a particle size less than or equal to 500μm, which is the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder.

[0060] The mineral additives are composed of major element minerals and trace element minerals. The major elements contained in the major element minerals are one or more of calcium, phosphorus, magnesium, sulfur, sodium, potassium, and chlorine. The trace elements contained in the trace element minerals are one or more of iron, copper, manganese, zinc, selenium, iodine, and cobalt.

[0061] The adsorbent is a molecular sieve.

[0062] The activated Yarrowia lipolytica fermentation sludge powder and the adsorbent composite powder are wetted with water to obtain wetted mud, and the amount of water added is 2.5% of the mass of the activated Yarrowia lipolytica fermentation sludge powder and the adsorbent composite powder. The wetted mud, the microbial agent and the mineral additive are uniformly mixed and stirred, and then dried to obtain dry blocks. The dry blocks are crushed and sieved to obtain granular functional microbial soil improver.

[0063] Example 3

[0064] The functional microbial soil improver based on Example 1 is used for breeding shrimps and crabs. The functional microbial soil improver is pre-embedded in the water bottom silt of the shrimp and crab breeding field by the pre-embedding method, and the amount of the functional microbial soil improver is 41±0.3 kg / hm 2 .

[0065] Comparative Example 1

[0066] The control soil improver is composed of a microbial agent and a molecular sieve. The microbial agent is a composite of bacillus, nitrifying bacteria, denitrifying bacteria, lactic acid bacteria, photosynthetic bacteria and butyric acid clostridium. The viable bacterial content in the control soil improver is 500 million to 3 billion per gram.

[0067] Comparative Example 2

[0068] The functional microbial soil improver comprises the following components:

[0069] Yarrowia lipolytica fermentation sludge powder and adsorbent mixed powder, microbial agent and mineral additive. The mass ratio of Yarrowia lipolytica fermentation sludge powder and adsorbent mixed powder to mineral additive is 100:5. The mass ratio of Yarrowia lipolytica fermentation sludge powder to adsorbent in the Yarrowia lipolytica fermentation sludge powder and adsorbent mixed powder is 100:17; the rest are the same as in Example 1.

[0070] Comparative Example 3

[0071] The difference between this example and Example 1 is that the activated Yarrowia lipolytica fermentation sludge powder and adsorbent mixed powder is used instead of the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder in this example. The activated Yarrowia lipolytica fermentation sludge powder refers to the fermentation sludge of Yarrowia lipolytica reacted in a liquid cell without the addition of adsorbent, and the final reaction obtains activated Yarrowia lipolytica fermentation sludge powder. The activated Yarrowia lipolytica fermentation sludge powder and adsorbent are mixed according to a mass ratio of 100:17 to obtain activated Yarrowia lipolytica fermentation sludge powder and adsorbent mixed powder. The rest are the same as in Example 1.

[0072] Stress resistance characterization test

[0073] In two shrimp ponds, one of the shrimp ponds was charged with 41±0.3kg / hm 2 The functional microbial substrate improver of Example 1 was pre-buried in the shrimp pond of Group A; the other shrimp pond was pre-buried at 41±0.3kg / hm 2 A control bottom modification agent was pre-embedded in the pond, designated Group B. The same batch of Litopenaeus vannamei was reared for 45 days. Samples were then taken from both Group A and Group B ponds and placed in two adjacent test ponds. The samples were then exposed to sunlight for three hours daily, with the water temperature maintained at 33°C. Any dead shrimp were removed during the exposure process. After two days of exposure, the number of dead shrimp was counted and the mortality rate calculated. This process was repeated, and the average was calculated.

[0074] The test found that the mortality rate of Penaeus vannamei cultured in Group A shrimp ponds after exposure to the sun was 11%; the mortality rate of Penaeus vannamei cultured in Group B shrimp ponds after exposure to the sun was 72%. This shows that the functional microbial substrate improver of the present invention can better adapt to extreme weather and water environments, and has strong stress resistance, especially strong resistance to heat stress.

[0075] Granule Yield Test

[0076] When preparing granules, in order to prevent excessive moisture from affecting the dormancy of bacteria in the microbial inoculant, spray wetting is used during the wetting process, and the amount of water added generally does not exceed 3%; based on these goals, the raw materials must have sufficient viscosity after wetting.

[0077] The sun-dried dry matter was broken up using a 3-bar hammer for 10 minutes. The broken material was sieved separately, and particles that passed a No. 1 sieve (2000 μm) but not a No. 5 sieve (180 μm) were collected as granules. Granule conversion rate = total granule weight / dry matter mass.

[0078] The test results of Example 1 and Comparative Examples 2 and 3 are shown in Table 1:

[0079] Table 1

[0080] Granule conversion rate / % Sediment oxidation-reduction potential / mV Example 1 87.5 The oxidation-reduction potential of the sediment at the pre-embedded site after 60 days was -131 Comparative Example 2 25.6 The oxidation-reduction potential of the sediment at the pre-embedded site after 60 days was -97 Comparative Example 3 20.9 The oxidation-reduction potential of the sediment at the pre-embedded site after 60 days was -102 Comparative Example 3

[0081] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0082] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A functional microbial substrate improver, characterized in that: Includes the following components: Activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder, microbial agent and mineral additive; The Yarrowia lipolytica fermentation sludge reacts in a liquid cell through a liquid-electric effect to obtain activated Yarrowia lipolytica fermentation sludge powder. The production method of the activated Yarrowia lipolytica fermentation sludge powder and an adsorbent composite powder comprises the following steps: Step 1: feeding the fermentation sludge of Yarrowia lipolytica into a liquid cell, adding water and an adsorbent into the liquid cell and stirring until a thin sludge is obtained, wherein the solid content of the thin sludge is 35% to 38%, the height of the thin sludge does not exceed half the height of the liquid cell, and the mass ratio of the fermentation sludge of Yarrowia lipolytica to the adsorbent is 100:17; Step 2: Cover the liquid battery with a cell cover. The cell cover is provided with multiple air holes. The ratio of the total area of ​​all air holes to the volume of the liquid battery is 15~20cm 2 / m 3 ; The power source connected to the electrodes of the liquid battery is pulse electricity, the voltage of the pulse electricity is 10kV, and the pulse frequency is 10~25Hz; after 11~12 minutes of liquid-electric treatment, the liquid-electric treatment is stopped, the pool cover is opened, and the material in the liquid battery is sucked into the filter press for filtration. The obtained filter cake is dried, crushed, and sieved to obtain a powder with a particle size of less than or equal to 500μm, which is the activated Yarrowia lipolytica fermentation sludge powder and adsorbent composite powder.

2. The functional microbial substrate improver according to claim 1, characterized in that: The mass ratio of the activated Yarrowia lipolytica fermentation sludge powder to the adsorbent composite powder and the mineral additive is 100:(5~10).

3. The functional microbial substrate improver according to claim 1, characterized in that: The microbial agent is one or more of nitrifying bacteria, denitrifying bacteria, and photosynthetic bacteria.

4. The functional microbial substrate improver according to claim 1, characterized in that: The content of live bacteria in the functional microbial substrate improver is 500 million to 3 billion per gram.

5. The functional microbial substrate improver according to claim 1, characterized in that: The mineral additives are composed of major element minerals and trace element minerals. The major elements contained in the major element minerals are one or more of calcium, phosphorus, magnesium, sulfur, sodium, potassium, and chlorine. The trace elements contained in the trace element minerals are one or more of iron, copper, manganese, zinc, selenium, iodine, and cobalt.

6. The functional microbial substrate improver according to claim 1, characterized in that: The adsorbent is a molecular sieve.

7. The method for preparing the functional microbial substrate improver according to any one of claims 1 to 6, wherein: The activated Yarrowia lipolytica fermentation sludge powder and the adsorbent composite powder are moistened with water to obtain a moistened mud material, wherein the amount of water added is 2.2% to 2.5% of the mass of the activated Yarrowia lipolytica fermentation sludge powder and the adsorbent composite powder. The moistened mud material is mixed and stirred evenly with a microbial agent and a mineral additive, and then dried, crushed, and sieved to obtain a granular functional microbial substrate improver.

8. Use of the functional microbial substrate improver according to any one of claims 1 to 6 in shrimp and crab farming.

9. The use of the functional microbial substrate modifier according to claim 8 in shrimp and crab farming, characterized in that: The functional microbial substrate improver is pre-buried in the bottom mud of the shrimp and crab farms by pre-buried method. The dosage of the functional microbial substrate improver is 41±0.3kg / hm 2 .