Anti-inflammatory repair composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to an anti-inflammatory repair composition as well as a preparation method and application thereof. The composition comprises a rhizoma anemarrhenae extract obtained by fermenting rhizoma anemarrhenae with eurotium cristatum. The composition provided by the invention enhances the moisture retention ability of the skin, helps to form a moisturizing barrier, and prevents moisture loss of the skin; the composition contains flavonoid and polysaccharide compounds, and can scavenge free radicals and play an anti-oxidation role, so that skin aging is slowed down, and the skin is protected from being damaged by environmental pressure. The formula can inhibit the formation of melanin, and is beneficial to improving uneven skin color and improving the brightness of the skin.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, and in particular to an anti-inflammatory repair composition and a preparation method and application thereof. Background Art

[0002] Anemarrhena asphodeloides is a common Chinese herbal medicine, mainly used in traditional Chinese medicine. It belongs to the Liliaceae family and usually grows in dry or semi-arid mountains and grasslands in China, Korea, and Mongolia. The rhizome of Anemarrhena contains a variety of bioactive ingredients, especially saponin compounds, which have significant pharmacological effects and are therefore widely used in traditional medicine.

[0003] The root of Anemarrhena asphodeloides is rich in a variety of bioactive ingredients, one of the most important of which is Anemarrhenasaponins, a class of compounds with strong pharmacological activity. Anemarrhena asphodeloides also contains some beneficial polysaccharides, flavonoids, volatile oils, amino acids and minerals.

[0004] Anemarrhena saponin is one of the main active ingredients in Anemarrhena, which has multiple pharmacological effects, such as anti-inflammatory, anti-tumor, and immune regulation. Anemarrhena polysaccharide is also one of the active ingredients, which has immune regulation and antioxidant effects. Flavonoids have anti-inflammatory, antioxidant, and antibacterial biological activities.

[0005] Anemarrhena has a significant effect of nourishing yin and moisturizing dryness, and can effectively improve dry and dehydrated skin. It can help the skin replenish moisture, enhance skin hydration, and keep the skin soft and smooth. For dry skin, dehydrated skin, or skin affected by dry climate, Anemarrhena extract is often used for moisturizing and repairing. This effect can be achieved through the polysaccharide components and saponin compounds in Anemarrhena, which have good hydration, can enhance the skin barrier function, lock in moisture, and reduce water loss.

[0006] The flavonoids and polysaccharides in Anemarrhena have antioxidant effects, which can effectively resist the damage of free radicals and slow down the aging process. Antioxidant ingredients can help neutralize oxidative damage caused by environmental factors such as ultraviolet rays, pollution, and stress on the skin surface, and reduce the rate of cell aging.

[0007] Studies have shown that Anemarrhena extract can promote the repair and regeneration of skin cells, improve skin elasticity and firmness, and thus fight against aging. It can also stimulate collagen synthesis and improve fine lines and sagging.

[0008] However, the water solubility of Anemarrhena saponins is poor. Saponin compounds usually have a certain degree of amphiphilicity, that is, they have both hydrophilic and hydrophobic structural parts. Anemarrhena saponins contain a glycosyl part and are hydrophilic, but because their molecules also contain a large number of hydrophobic steroid skeletons, their overall water solubility is poor. At the same time, Anemarrhena extract is more sensitive to pH values ​​and requires a relatively high applicable environment and storage environment.

[0009] To this end, the present invention provides an anti-inflammatory repair composition and a preparation method and application thereof. Summary of the invention

[0010] In order to solve the above technical problems, the present invention provides an anti-inflammatory repair composition, which comprises an Anemarrhena Rhizoma extract obtained by fermenting Anemarrhena Rhizoma with Psoralea corylifolia.

[0011] A second aspect of the present invention provides a repair antioxidant composition, which comprises an Anemarrhena Rhizoma extract obtained by fermenting Anemarrhena Rhizoma with Psoralea corylifolia.

[0012] As an embodiment of the present invention, the composition further comprises tea extract, Glycyrrhiza glabra extract, and Hedyotis diffusa extract.

[0013] As an embodiment of the present invention, the mass ratio of the Anemarrhena asphodeloides extract, the Camellia oleifera extract, the Glycyrrhiza glabra extract and the Oldenlandia diffusa extract is: 1-3: 5-15: 2-8: 1-3.

[0014] As an embodiment of the present invention, the mass ratio of the Anemarrhena asphodeloides extract, the Tea extract, the Glycyrrhiza glabra extract, and the Oldenlandia diffusa extract is 3:10:6:2.

[0015] As an embodiment of the present invention, the preparation method of the Anemarrhena asphodeloides extract is as follows:

[0016] S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow;

[0017] S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside;

[0018] S03 Preparation of liquid seed solution: inoculate the activated bacteria into a liquid culture medium containing glucose and yeast extract, and culture in a constant temperature shaker at 28° C. and 150 rpm for 48 hours to prepare a seed solution.

[0019] S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0020] S05 Adding symbiotic bacteria in the middle stage: On the third day of liquid fermentation, add activated yeast and continue to culture for 3 to 4 days;

[0021] S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0022] S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0023] As an embodiment of the present invention, the components of the liquid culture medium in S04 include:

[0024] Glucose 20-30g / L, peptone 5-10g / L, yeast extract 2-5g / L, ammonium nitrate 1-2g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0025] The third aspect of the present invention provides a skin care product comprising the repairing and antioxidant composition.

[0026] As an embodiment of the present invention, the raw materials for preparing the skin care product include:

[0027] Glycerin: 4 parts

[0028] p-Hydroxyacetophenone: 0.3 parts

[0029] Carbomer: 0.6 parts

[0030] Arginine: 0.6 parts

[0031] The repair antioxidant composition: 10-15 parts

[0032] 1,2-Hexanediol: 0.5 parts

[0033] Water was added to 100 parts.

[0034] The fourth aspect of the present invention provides the composition, which is applied in the field of daily chemicals.

[0035] By adopting the above technical solution, the present invention has the following beneficial effects:

[0036] The present invention provides a composition that enhances the moisture retention capacity of the skin, helps form a moisturizing barrier, and prevents moisture loss from the skin; the composition contains flavonoids and polysaccharide compounds, which can scavenge free radicals and play an antioxidant role, thereby slowing down skin aging and protecting the skin from damage caused by environmental stress.

[0037] The formula of the present invention inhibits the formation of melanin, helps to improve uneven skin tone and enhance skin brightness. DETAILED DESCRIPTION

[0038] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] The invention provides a repairing antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of Tea Leaves, an extract of Glycyrrhiza glabra and an extract of Hedyotis diffusa, and the mass ratio is 3:10:6:2.

[0040] As an embodiment of the present invention, the preparation method of the Anemarrhena asphodeloides extract is as follows:

[0041] S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow;

[0042] S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside;

[0043] S03 Preparation of liquid seed solution: inoculate the activated bacteria into a liquid culture medium containing glucose and yeast extract, and culture in a constant temperature shaker at 28° C. and 150 rpm for 48 hours to prepare a seed solution.

[0044] S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0045] S05 mid-term addition of symbiotic bacteria: On the third day of liquid fermentation, add green tea powder and activated yeast (inoculation 3%), and continue to culture for 3 to 4 days;

[0046] S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0047] S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0048] As an embodiment of the present invention, the components of the liquid culture medium in S04 include:

[0049] Glucose 20-30g / L, peptone 5-10g / L, yeast extract 2-5g / L, ammonium nitrate 1-2g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0050] Among them, the golden flower fungus is Eurotium cristatum, China Industrial Microorganism Culture Collection Management Center.

[0051] The yeast is Rhodosporidium toruloides. China Industrial Microorganism Culture Collection Management Center.

[0052] The mass ratio of Anemarrhena asphodeloides powder to green tea powder is 3:1.

[0053] As an embodiment of the present invention, the tea leaves are black tea and white tea, and the mass ratio is 2:3. The method for extracting the tea leaves is as follows:

[0054] Grinding: Grind the black tea leaves / white tea leaves and pass through 100 mesh;

[0055] The crushed tea powder was suspended in a buffer solution at 50°C (0.1 M citric acid-phosphate buffer, pH 4.5-5.5), cellulase and pectinase (total mass 8%, mass ratio 3:5) were added, and enzymatic hydrolysis was performed for 30 minutes;

[0056] Ultrasonic extraction: The tea suspension after enzymatic hydrolysis was placed in an ultrasonic extractor and treated at a frequency of 40 kHz and 50°C for 30 minutes.

[0057] Microwave extraction: immediately transfer the solution to a microwave extraction device and heat it at 300 W for 5 minutes; then use CO2 as a supercritical fluid, set the temperature to 35°C, the pressure to 25 MPa, and use 10 vol% ethanol as a co-solvent; the CO2 flow rate is set at 10 mL / min; extraction time: 30 minutes; collect the extracted components, use high-speed centrifugation (10,000 rpm) to remove the residue, and use a rotary evaporator to concentrate the extract; dry the concentrated extract into powder by freeze drying to obtain the tea extract.

[0058] In the present invention, the preparation method of the Glycyrrhiza glabra is as follows:

[0059] S1: Glycyrrhiza glabra powder is loaded into an extraction tank; supercritical CO2 and 5 vol% ethanol are used as co-solvents to extract at 30°C and 25 MPa for 60 minutes; the CO2 flow rate is set at 10 mL / min; the extract is collected and concentrated; the concentrated extract is dried into powder by freeze drying;

[0060] S2: Add 5 times the volume of distilled water to the powder obtained in step S1, and stir it thoroughly with a stirrer to disperse it in the water; while stirring, slowly add 1 / 8 volume of potassium hydroxide aqueous solution (10wt%).

[0061] S3 is stirred continuously at 40° C. for 2 h, then concentrated under reduced pressure to remove excess water; and dried to obtain the Glycyrrhiza glabra extract.

[0062] In the present invention, the preparation method of the Hedyotis diffusa extract is as follows:

[0063] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0064] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0065] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0066] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0067] As an embodiment of the present invention, the repair antioxidant composition further comprises honey.

[0068] The third aspect of the present invention provides a skin care product comprising the repairing and antioxidant composition.

[0069] As an embodiment of the present invention, the raw materials for preparing the skin care product include:

[0070] Glycerin: 4 parts

[0071] p-Hydroxyacetophenone: 0.3 parts

[0072] Carbomer: 0.6 parts

[0073] Arginine: 0.6 parts

[0074] The repair antioxidant composition: 10-15 parts

[0075] 1,2-Hexanediol: 0.5 parts

[0076] Water was added to 100 parts.

[0077] The preparation method is as follows:

[0078] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0079] Rhizoma Anemarrhenae has antioxidant and whitening effects on the skin, but its main effects are Rhizoma Anemarrhenae polysaccharides and Rhizoma Anemarrhenae saponins, both of which have certain challenges to the absorption of the skin; at the same time, the Rhizoma Anemarrhenae extract has high requirements for pH value; for this reason, the present invention uses golden flower fungus to ferment Rhizoma Anemarrhenae; at the same time, Glycyrrhiza glabra and Hedyotis diffusa extracts are added, and the irritation of Glycyrrhiza glabra and Hedyotis diffusa extracts can be effectively solved by combining Rhizoma Anemarrhenae and tea leaves; at the same time, after the fermented Rhizoma Anemarrhenae extract and other components are mixed, it is not easy to produce precipitation. Because saponin compounds (such as Rhizoma Anemarrhenae saponins in Rhizoma Anemarrhenae) are usually more soluble under acidic conditions, but under alkaline conditions, their solubility will decrease and precipitation may be formed. Glycyrrhizic acid (the main component in Glycyrrhiza glabra) is relatively stable in neutral or slightly alkaline environments, but precipitation may also occur in extreme acidic or alkaline environments. At the same time, the combination or coordination between components such as phenols, flavonoids, and saponins may lead to the formation of some insoluble complexes. For example, the flavonoids in Hedyotis diffusa may interact with certain components in Glycyrrhiza glabra, thereby affecting their solubility. The method used in the present invention can effectively avoid the above phenomenon.

[0080] The present invention is further explained below in conjunction with specific implementation modes.

[0081] Example 1

[0082] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of tea leaves, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:10:6:2:1.

[0083] The preparation method of the Anemarrhena Rhizoma extract is as follows:

[0084] S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow;

[0085] S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside;

[0086] S03 Preparation of liquid seed solution: Inoculate the activated golden flower bacteria into the liquid culture medium, and culture it in a constant temperature shaker at 28°C and 150rpm for 48 hours to prepare 10 7 CFU / mL Jinhua strain liquid.

[0087] S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0088] S05 mid-term addition of symbiotic bacteria: on the third day of liquid fermentation, add green tea powder and yeast culture solution (inoculation 3%), and continue to culture for 3 to 4 days;

[0089] S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0090] S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0091] The components of the liquid culture medium in S03 and S04 include:

[0092] Glucose 25g / L, peptone 8g / L, yeast extract 2.5g / L, ammonium nitrate 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0093] Among them, the golden flower fungus is Eurotium cristatum, China Industrial Microorganism Culture Collection Management Center.

[0094] The yeast is Rhodosporidium toruloides. China Industrial Microorganism Culture Collection Management Center.

[0095] The mass ratio of Anemarrhena asphodeloides powder and green tea powder is 3:1, and both are passed through 200 mesh.

[0096] In this embodiment, the tea leaves are a mixture of black tea and white tea, and the mass ratio is 2:3. The method for extracting the tea leaves is as follows:

[0097] Grinding: Grind the black tea leaves / white tea leaves and pass through 100 mesh;

[0098] The crushed tea powder was suspended in a 10-fold mass of a buffer solution at 50°C (0.1 M citric acid-phosphate buffer, pH 4.5-5.5), cellulase and pectinase (total mass 8%, mass ratio 3:5) were added, and enzymatic treatment was performed for 30 minutes;

[0099] Ultrasonic extraction: The tea suspension after enzymatic hydrolysis was placed in an ultrasonic extractor and treated at a frequency of 40 kHz and 50°C for 30 minutes.

[0100] Microwave extraction: immediately transfer the solution to a microwave extraction device and heat it at 300 W for 5 minutes; then use CO2 as a supercritical fluid, set the temperature to 35°C, the pressure to 25 MPa, and use 10 vol% ethanol as a co-solvent; the CO2 flow rate is set at 10 mL / min; extraction time: 30 minutes; collect the extracted components, use high-speed centrifugation (10,000 rpm) to remove the residue, and use a rotary evaporator to concentrate the extract; dry the concentrated extract into powder by freeze drying to obtain the tea extract.

[0101] In this embodiment, the preparation method of the Glycyrrhiza glabra is as follows:

[0102] S1: Glycyrrhiza glabra powder is loaded into an extraction tank; supercritical CO2 and 5 vol% ethanol are used as co-solvents to extract at 30°C and 25 MPa for 60 minutes; the CO2 flow rate is set at 10 mL / min; the extract is collected and concentrated; the concentrated extract is dried into powder by freeze drying;

[0103] S2: Add 5 times the volume of distilled water to the powder obtained in step S1, and stir it thoroughly with a stirrer to disperse it in the water; while stirring, slowly add 1 / 8 volume of potassium hydroxide aqueous solution (10wt%).

[0104] S3 is stirred continuously at 40° C. for 2 h, then concentrated under reduced pressure to remove excess water; and dried to obtain the Glycyrrhiza glabra extract.

[0105] In this embodiment, the preparation method of the Hedyotis diffusa extract is as follows:

[0106] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0107] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0108] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0109] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0110] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0111] Glycerin: 4 parts

[0112] p-Hydroxyacetophenone: 0.3 parts

[0113] Carbomer: 0.6 parts

[0114] Arginine: 0.6 parts

[0115] The repair antioxidant composition: 10-15 parts

[0116] 1,2-Hexanediol: 0.5 parts

[0117] Water was added to 100 parts.

[0118] The preparation method is as follows:

[0119] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0120] Example 2

[0121] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of tea leaves, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:10:6:2:1.

[0122] The preparation of the Anemarrhena extract is as follows: Anemarrhena powder and green tea powder are mixed in a mass ratio of 3:1, and then water-extracted at a solid-liquid ratio of 1:10 at a temperature of 60°C for 2 hours, and then concentrated by reduced pressure distillation to a volume equal to that of Example 1.

[0123] In this embodiment, the tea leaves are a mixture of black tea and white tea, and the mass ratio is 2:3. The method for extracting the tea leaves is as follows:

[0124] Grinding: Grind the black tea leaves / white tea leaves and pass through 100 mesh;

[0125] The crushed tea powder was suspended in a 10-fold mass of a buffer solution at 50°C (0.1 M citric acid-phosphate buffer, pH 4.5-5.5), cellulase and pectinase (total mass 8%, mass ratio 3:5) were added, and enzymatic treatment was performed for 30 minutes;

[0126] Ultrasonic extraction: The tea suspension after enzymatic hydrolysis was placed in an ultrasonic extractor and treated at a frequency of 40 kHz and 50°C for 30 minutes.

[0127] Microwave extraction: immediately transfer the solution to a microwave extraction device and heat it at 300 W for 5 minutes; then use CO2 as a supercritical fluid, set the temperature to 35°C, the pressure to 25 MPa, and use 10 vol% ethanol as a co-solvent; the CO2 flow rate is set at 10 mL / min; extraction time: 30 minutes; collect the extracted components, use high-speed centrifugation (10,000 rpm) to remove the residue, and use a rotary evaporator to concentrate the extract; dry the concentrated extract into powder by freeze drying to obtain the tea extract.

[0128] In this embodiment, the preparation method of the Glycyrrhiza glabra is as follows:

[0129] S1: Glycyrrhiza glabra powder is loaded into an extraction tank; supercritical CO2 and 5 vol% ethanol are used as co-solvents to extract at 30°C and 25 MPa for 60 minutes; the CO2 flow rate is set at 10 mL / min; the extract is collected and concentrated; the concentrated extract is dried into powder by freeze drying;

[0130] S2: Add 5 times the volume of distilled water to the powder obtained in step S1, and stir it thoroughly with a stirrer to disperse it in the water; while stirring, slowly add 1 / 8 volume of potassium hydroxide aqueous solution (10wt%).

[0131] S3 is stirred continuously at 40° C. for 2 h, then concentrated under reduced pressure to remove excess water; and dried to obtain the Glycyrrhiza glabra extract.

[0132] In this embodiment, the preparation method of the Hedyotis diffusa extract is as follows:

[0133] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0134] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0135] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0136] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0137] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0138] Glycerin: 4 parts

[0139] p-Hydroxyacetophenone: 0.3 parts

[0140] Carbomer: 0.6 parts

[0141] Arginine: 0.6 parts

[0142] The repair antioxidant composition: 10-15 parts

[0143] 1,2-Hexanediol: 0.5 parts

[0144] Water was added to 100 parts.

[0145] The preparation method is as follows:

[0146] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0147] Example 3

[0148] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of tea leaves, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:10:6:2:1.

[0149] The preparation method of the Anemarrhena Rhizoma extract is as follows:

[0150] S01 yeast was cultured in YPD medium for 24 h and set aside;

[0151] S02 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, and inoculate 5% yeast; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0152] S03 mid-stage culture: On the third day of liquid fermentation, add green tea powder and continue culturing for 3 to 4 days;

[0153] S04 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0154] S05 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under reduced pressure at low temperature, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0155] The components of the liquid culture medium in S02 include:

[0156] Glucose 25g / L, peptone 8g / L, yeast extract 2.5g / L, ammonium nitrate 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0157] The yeast is Rhodosporidium toruloides. China Industrial Microorganism Culture Collection Management Center.

[0158] The mass ratio of Anemarrhena asphodeloides powder and green tea powder is 3:1, and both are passed through 200 mesh.

[0159] In this embodiment, the tea leaves are a mixture of black tea and white tea, and the mass ratio is 2:3. The method for extracting the tea leaves is as follows:

[0160] Grinding: Grind the black tea leaves / white tea leaves and pass through 100 mesh;

[0161] The crushed tea powder was suspended in a 10-fold mass of a buffer solution at 50°C (0.1 M citric acid-phosphate buffer, pH 4.5-5.5), cellulase and pectinase (total mass 8%, mass ratio 3:5) were added, and enzymatic treatment was performed for 30 minutes;

[0162] Ultrasonic extraction: The tea suspension after enzymatic hydrolysis was placed in an ultrasonic extractor and treated at a frequency of 40 kHz and 50°C for 30 minutes.

[0163] Microwave extraction: immediately transfer the solution to a microwave extraction device and heat it at 300 W for 5 minutes; then use CO2 as a supercritical fluid, set the temperature to 35°C, the pressure to 25 MPa, and use 10 vol% ethanol as a co-solvent; the CO2 flow rate is set at 10 mL / min; extraction time: 30 minutes; collect the extracted components, use high-speed centrifugation (10,000 rpm) to remove the residue, and use a rotary evaporator to concentrate the extract; dry the concentrated extract into powder by freeze drying to obtain the tea extract.

[0164] In this embodiment, the preparation method of the Glycyrrhiza glabra is as follows:

[0165] S1: Glycyrrhiza glabra powder is loaded into an extraction tank; supercritical CO2 and 5 vol% ethanol are used as co-solvents to extract at 30°C and 25 MPa for 60 minutes; the CO2 flow rate is set at 10 mL / min; the extract is collected and concentrated; the concentrated extract is dried into powder by freeze drying;

[0166] S2: Add 5 times the volume of distilled water to the powder obtained in step S1, and stir it thoroughly with a stirrer to disperse it in the water; while stirring, slowly add 1 / 8 volume of potassium hydroxide aqueous solution (10wt%).

[0167] S3 is stirred continuously at 40° C. for 2 h, then concentrated under reduced pressure to remove excess water; and dried to obtain the Glycyrrhiza glabra extract.

[0168] In this embodiment, the preparation method of the Hedyotis diffusa extract is as follows:

[0169] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0170] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0171] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0172] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0173] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0174] Glycerin: 4 parts

[0175] p-Hydroxyacetophenone: 0.3 parts

[0176] Carbomer: 0.6 parts

[0177] Arginine: 0.6 parts

[0178] The repair antioxidant composition: 10-15 parts

[0179] 1,2-Hexanediol: 0.5 parts

[0180] Water was added to 100 parts.

[0181] The preparation method is as follows:

[0182] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0183] Example 4

[0184] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of tea leaves, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:10:6:2:1.

[0185] The preparation method of the Anemarrhena Rhizoma extract is as follows:

[0186] S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow;

[0187] S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside;

[0188] S03 Preparation of liquid seed solution: Inoculate the activated golden flower bacteria into the liquid culture medium, and culture it in a constant temperature shaker at 28°C and 150rpm for 48 hours to prepare 10 7 CFU / mL Jinhua strain liquid.

[0189] S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0190] S05 mid-term addition of symbiotic bacteria: on the third day of liquid fermentation, add yeast (inoculation 3%) and continue to culture for 3 to 4 days;

[0191] S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0192] S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0193] The components of the liquid culture medium in S03 and S04 include:

[0194] Glucose 25g / L, peptone 8g / L, yeast extract 2.5g / L, ammonium nitrate 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0195] Among them, the golden flower fungus is Eurotium cristatum, China Industrial Microorganism Culture Collection Management Center.

[0196] The yeast is Rhodosporidium toruloides. China Industrial Microorganism Culture Collection Management Center.

[0197] The mass ratio of Anemarrhena asphodeloides powder and green tea powder is 3:1, and both are passed through 200 mesh.

[0198] In this embodiment, the tea leaves are a mixture of black tea and white tea, and the mass ratio is 2:3. The method for extracting the tea leaves is as follows:

[0199] Grinding: Grind the black tea leaves / white tea leaves and pass through 100 mesh;

[0200] The crushed tea powder was suspended in a 10-fold mass of a buffer solution at 50°C (0.1 M citric acid-phosphate buffer, pH 4.5-5.5), cellulase and pectinase (total mass 8%, mass ratio 3:5) were added, and enzymatic treatment was performed for 30 minutes;

[0201] Ultrasonic extraction: The tea suspension after enzymatic hydrolysis was placed in an ultrasonic extractor and treated at a frequency of 40 kHz and 50°C for 30 minutes.

[0202] Microwave extraction: immediately transfer the solution to a microwave extraction device and heat it at 300 W for 5 minutes; then use CO2 as a supercritical fluid, set the temperature to 35°C, the pressure to 25 MPa, and use 10 vol% ethanol as a co-solvent; the CO2 flow rate is set at 10 mL / min; extraction time: 30 minutes; collect the extracted components, use high-speed centrifugation (10,000 rpm) to remove the residue, and use a rotary evaporator to concentrate the extract; dry the concentrated extract into powder by freeze drying to obtain the tea extract.

[0203] In this embodiment, the preparation method of the Glycyrrhiza glabra is as follows:

[0204] S1: Glycyrrhiza glabra powder is loaded into an extraction tank; supercritical CO2 and 5 vol% ethanol are used as co-solvents to extract at 30°C and 25 MPa for 60 minutes; the CO2 flow rate is set at 10 mL / min; the extract is collected and concentrated; the concentrated extract is dried into powder by freeze drying;

[0205] S2: Add 5 times the volume of distilled water to the powder obtained in step S1, and stir it thoroughly with a stirrer to disperse it in the water; while stirring, slowly add 1 / 8 volume of potassium hydroxide aqueous solution (10wt%).

[0206] S3 is stirred continuously at 40° C. for 2 h, then concentrated under reduced pressure to remove excess water; and dried to obtain the Glycyrrhiza glabra extract.

[0207] In this embodiment, the preparation method of the Hedyotis diffusa extract is as follows:

[0208] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0209] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0210] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0211] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0212] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0213] Glycerin: 4 parts

[0214] p-Hydroxyacetophenone: 0.3 parts

[0215] Carbomer: 0.6 parts

[0216] Arginine: 0.6 parts

[0217] The repair antioxidant composition: 10-15 parts

[0218] 1,2-Hexanediol: 0.5 parts

[0219] Water was added to 100 parts.

[0220] The preparation method is as follows:

[0221] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0222] Example 5

[0223] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:6:2:1.

[0224] The preparation method of the Anemarrhena Rhizoma extract is as follows:

[0225] S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow;

[0226] S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside;

[0227] S03 Preparation of liquid seed solution: Inoculate the activated golden flower bacteria into the liquid culture medium, and culture it in a constant temperature shaker at 28°C and 150rpm for 48 hours to prepare 10 7 CFU / mL Jinhua strain liquid.

[0228] S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0229] S05 mid-term addition of symbiotic bacteria: on the third day of liquid fermentation, add green tea powder and yeast culture solution (inoculation 3%), and continue to culture for 3 to 4 days;

[0230] S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0231] S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0232] The components of the liquid culture medium in S03 and S04 include:

[0233] Glucose 25g / L, peptone 8g / L, yeast extract 2.5g / L, ammonium nitrate 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0234] Among them, the golden flower fungus is Eurotium cristatum, China Industrial Microorganism Culture Collection Management Center.

[0235] The yeast is Rhodosporidium toruloides. China Industrial Microorganism Culture Collection Management Center.

[0236] The mass ratio of Anemarrhena asphodeloides powder and green tea powder is 3:1, and both are passed through 200 mesh.

[0237] In this embodiment, the preparation method of the Glycyrrhiza glabra is as follows:

[0238] S1: Glycyrrhiza glabra powder is loaded into an extraction tank; supercritical CO2 and 5 vol% ethanol are used as co-solvents to extract at 30°C and 25 MPa for 60 minutes; the CO2 flow rate is set at 10 mL / min; the extract is collected and concentrated; the concentrated extract is dried into powder by freeze drying;

[0239] S2: Add 5 times the volume of distilled water to the powder obtained in step S1, and stir it thoroughly with a stirrer to disperse it in the water; while stirring, slowly add 1 / 8 volume of potassium hydroxide aqueous solution (10wt%).

[0240] S3 is stirred continuously at 40° C. for 2 h, then concentrated under reduced pressure to remove excess water; and dried to obtain the Glycyrrhiza glabra extract.

[0241] In this embodiment, the preparation method of the Hedyotis diffusa extract is as follows:

[0242] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0243] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0244] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0245] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0246] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0247] Glycerin: 4 parts

[0248] p-Hydroxyacetophenone: 0.3 parts

[0249] Carbomer: 0.6 parts

[0250] Arginine: 0.6 parts

[0251] The repair antioxidant composition: 10-15 parts

[0252] 1,2-Hexanediol: 0.5 parts

[0253] Water was added to 100 parts.

[0254] The preparation method is as follows:

[0255] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0256] Example 6

[0257] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of tea leaves, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:10:6:2:1.

[0258] The preparation method of the Anemarrhena Rhizoma extract is as follows:

[0259] S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow;

[0260] S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside;

[0261] S03 Preparation of liquid seed solution: Inoculate the activated golden flower bacteria into the liquid culture medium, and culture it in a constant temperature shaker at 28°C and 150rpm for 48 hours to prepare 10 7 CFU / mL Jinhua strain liquid.

[0262] S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days;

[0263] S05 mid-term addition of symbiotic bacteria: on the third day of liquid fermentation, add green tea powder and yeast culture solution (inoculation 3%), and continue to culture for 3 to 4 days;

[0264] S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days;

[0265] S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

[0266] The components of the liquid culture medium in S03 and S04 include:

[0267] Glucose 25g / L, peptone 8g / L, yeast extract 2.5g / L, ammonium nitrate 0.5g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

[0268] Among them, the golden flower fungus is Eurotium cristatum, China Industrial Microorganism Culture Collection Management Center.

[0269] The yeast is Rhodosporidium toruloides. China Industrial Microorganism Culture Collection Management Center.

[0270] The mass ratio of Anemarrhena asphodeloides powder and green tea powder is 3:1, and both are passed through 200 mesh.

[0271] In this embodiment, the tea leaves are a mixture of black tea and white tea, and the mass ratio is 2:3. The method for extracting the tea leaves is as follows:

[0272] Grinding: Grind the black tea leaves / white tea leaves and pass through 100 mesh;

[0273] The crushed tea powder was suspended in a 10-fold mass of a buffer solution at 50°C (0.1 M citric acid-phosphate buffer, pH 4.5-5.5), cellulase and pectinase (total mass 8%, mass ratio 3:5) were added, and enzymatic treatment was performed for 30 minutes;

[0274] Ultrasonic extraction: The tea suspension after enzymatic hydrolysis was placed in an ultrasonic extractor and treated at a frequency of 40 kHz and 50°C for 30 minutes.

[0275] Microwave extraction: immediately transfer the solution to a microwave extraction device and heat it at 300 W for 5 minutes; then use CO2 as a supercritical fluid, set the temperature to 35°C, the pressure to 25 MPa, and use 10 vol% ethanol as a co-solvent; the CO2 flow rate is set at 10 mL / min; extraction time: 30 minutes; collect the extracted components, use high-speed centrifugation (10,000 rpm) to remove the residue, and use a rotary evaporator to concentrate the extract; dry the concentrated extract into powder by freeze drying to obtain the tea extract.

[0276] In this embodiment, the preparation method of the Glycyrrhiza glabra is as follows:

[0277] S1: Loading the Glycyrrhiza glabra powder into an extraction tank; using supercritical CO2 and 5 vol% ethanol as co-solvents to extract at 30°C and 25 MPa for 60 minutes; setting the CO2 flow rate at 10 mL / min; collecting and concentrating the extract; drying the concentrated extract into powder by freeze drying; and obtaining the Glycyrrhiza glabra extract.

[0278] In this embodiment, the preparation method of the Hedyotis diffusa extract is as follows:

[0279] (1) Sample preparation: Grind the Hedyotis diffusa sample into fine particles

[0280] (2) adding 10 times the amount of deionized water to the Hedyotis diffusa powder, and heating the extract at 80° C. with stirring for 1-2 hours;

[0281] (3) After the extraction is completed, the extract is cooled to room temperature and filtered to remove plant residues;

[0282] (4) The filtered extract is placed in a rotary evaporator and concentrated under reduced pressure at 40°C to 1 / 5 of its volume, and the concentrate is freeze-dried.

[0283] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0284] Glycerin: 4 parts

[0285] p-Hydroxyacetophenone: 0.3 parts

[0286] Carbomer: 0.6 parts

[0287] Arginine: 0.6 parts

[0288] The repair antioxidant composition: 10-15 parts

[0289] 1,2-Hexanediol: 0.5 parts

[0290] Water was added to 100 parts.

[0291] The preparation method is as follows:

[0292] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0293] Example 7

[0294] The present embodiment provides a repair antioxidant composition, which comprises an extract of Anemarrhena asphodeloides, an extract of tea leaves, an extract of Glycyrrhiza glabra, an extract of Hedyotis diffusa, and honey; and the mass ratio is 3:10:6:2:1.

[0295] The preparation methods of the Anemarrhena Rhizoma extract, tea extract, Glycyrrhiza glabra extract and Hedyotis diffusa extract are as follows:

[0296] S01: Mix and grind Rhizoma Anemarrhenae, tea leaves, Glycyrrhiza glabra and Hedyotis diffusa into fine particles;

[0297] S02 added 10 times the amount of deionized water, and heated the extract at 80°C with stirring for 1-2 hours;

[0298] After S03 extraction is completed, cool to room temperature, filter the extract, and remove plant residues;

[0299] S04: Place the filtered extract into a rotary evaporator, concentrate the extract to 1 / 5 of its volume at 40°C under reduced pressure, and freeze-dry the concentrate.

[0300] This embodiment also provides a skin care product, the preparation raw materials of which include:

[0301] Glycerin: 4 parts

[0302] p-Hydroxyacetophenone: 0.3 parts

[0303] Carbomer: 0.6 parts

[0304] Arginine: 0.6 parts

[0305] The repair antioxidant composition: 10-15 parts

[0306] 1,2-Hexanediol: 0.5 parts

[0307] Water was added to 100 parts.

[0308] The preparation method is as follows:

[0309] Mix water, glycerin, p-hydroxyacetophenone and carbomer, stir and heat to 75-80 degrees, homogenize for 2-3 minutes until completely dissolved, start to cool to below 45 degrees, add arginine, stir slowly until transparent, add remaining ingredients and stir well.

[0310] Performance Testing

[0311] Test 1: The products prepared in Examples 1 to 7 were placed at 5°C, 25°C, and 40°C for 90 days to check the product properties. The test results are shown in Table 1.

[0312] Table 1 Test results of performance test 1

[0313] Example 5℃ 25℃ 40℃ Example 1 constant constant constant Example 2 Partial turbidity constant Partial water discharge Example 3 constant constant Partial water discharge Example 4 Layering Partial turbidity Layering Example 5 Layering Layering Layering Example 6 Layering Layering Layering Example 7 Layering Layering Layering

[0314] Test 2: Elastase inhibition rate

[0315] Fermentation filtrate: dilute with pure water to a sample concentration of 5%;

[0316] Positive control (epigallocatechin gallate): diluted with water to a positive control concentration of 0.1%;

[0317] Negative control: pure water.

[0318] Test operation steps:

[0319] Design the sample group, sample background group, solvent group and solvent background group. Set up 3 parallels for each group. Add different reagent solutions into the 96-well plate, shake gently, incubate at 25°C for 15 minutes, place it in the microplate reader, and measure the absorbance at 410nm.

[0320] Calculation formula: Elastase inhibition rate (%) = (1-(CD) / (AB)) × 100

[0321] Wherein, A is the absorbance of the reaction solution without sample; B is the absorbance of the reaction solution without sample and enzyme; C is the absorbance of the reaction solution containing sample and enzyme; D is the absorbance of the reaction solution containing sample and no enzyme.

[0322] The statistical analysis software was SPSS, and the comparison of the elastase inhibition rates of the test samples, positive controls and negative controls was performed using an independent sample t test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05, P > 0.05, indicating that there was no significant difference between the two groups; P < 0.05, indicating that there was a significant difference between the two groups.

[0323] Table 2 Test results

[0324] name Test results (%) P-value Example 1 68.243±0.153 ＜0.05 Example 2 60.642±0.275 ＜0.05 Example 3 65.269±0.361 ＜0.05 Example 4 62.163±0.637 ＜0.05 Example 5 53.283±0.163 ＜0.05 Example 6 58.053±0.227 ＜0.05 Example 7 51.141±0.226 ＜0.05 Negative control -8.127±1.326 / Positive Control 70.321±7.356 ＜0.05

[0325] The elastase inhibition rate was rounded to three decimal places, and P < 0.05 indicated that there were significant differences between the sample and the positive control and the negative control.

[0326] Test 3: Transepidermal Water Loss Rate Difference

[0327] Healthy subjects aged 25-50 years old (all subjects already have sensitive skin characteristics, such as allergies, redness, etc.), including 7 males and 35 females, with an average age of 37.32 years old.

[0328] Part used: hand and forearm

[0329] Usage: When used on the forearm, the test sample is (2.0±0.1) mg / cm 2 Apply a single application of the dosage and use a latex finger cot to evenly spread the sample in the test area.

[0330] Test steps

[0331] The subjects did not touch the inner part of their arms with water before the visit.

[0332] Before the experiment, the subjects were required to uniformly clean their arms with dry facial tissue;

[0333] Mark two measurement areas on the inside of the subject's left or right arm, each area is 3cm*3cm, and the distance between the areas is at least 1cm;

[0334] The sample area and negative control area were randomly distributed in the calibration area to ensure that each area was statistically balanced: the subjects exposed their arms and rested for 30 minutes in an environment with a temperature of 21±1°C and a relative humidity of 50±10%RH; the inducer (0.1% histamine solution) was applied to the sample area and the negative area for 10 minutes, and after the application and before the use of the sample (Timm), the transepidermal water loss rate of the calibration area was tested with Tewameter, and the hemoglobin of the calibration area was tested with Mexamter;

[0335] Use the samples in the designated sample area according to the sample usage instructions;

[0336] After using the sample, the subjects rested their arms in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH for 1 hour. After 1 hour (T1), the transepidermal water loss rate of the calibrated area was tested with a Tewameter.

[0337] Transepidermal water loss rate difference

[0338] Courage+Khazaka Transepidermal Water Loss and Evaporative Heat Loss Test Probe Tewameter TM300 is used to measure the transepidermal water loss rate of the skin.

[0339] Table 3 Comparison of transepidermal water loss rate before and after

[0340]

[0341]

[0342] Test 4: Anti-inflammatory test

[0343] This test uses lipopolysaccharide (LPS) to stimulate giant cell Raw264.7 to establish an inflammatory model, and evaluates the soothing effect of the sample by detecting the changes in the secretion of related inflammatory factors after the sample is exposed.

[0344] The cells used in this test were macrophage Raw264.7 (mouse giant cell line), purchased from the Cell Bank of the Chinese Academy of Sciences.

[0345] Cell seeding: 6.4*10 5 The cells were seeded into 6-well plates at a seeding density of 100 cells / well and incubated overnight in an incubator (37°C, 5% CO2).

[0346] 2. Liquid preparation: Prepare the working solution of the test substance according to the experimental design (Table 4).

[0347] Table 4

[0348]

[0349] Drug administration: When the cell plating rate in the 6-well plate reaches 40% to 60%, group drug administration is carried out, and the dosage per well is 1.8 mL. Each group has 3 replicate wells, and the culture is continued in the incubator (37°C, 5% CO2) for 2 hours.

[0350] LPS stimulation: After 2 hours of culture, add 200uL of LPS working solution prepared from the corresponding test substance working solution to the drug-treated wells according to the experimental design, shake the wells left and right to mix the drugs in the wells, and the final LPS concentration is 1ug / mL. Continue to culture in the incubator (37°C, 5% CO2) for 22 hours.

[0351] Sample collection: After the incubation, collect the cell culture supernatant in an EP tube (Note: the amount of sample collected is determined according to the test index), and store the sample in a -80°C refrigerator. The IL-1α content is detected according to the operating instructions of the Mouse IL-1α ELISA kit. The PGE2 content is detected according to the operating instructions of the PGE2 ELISA kit.

[0352] Table 5 Test results

[0353]

[0354]

[0355] Significance is indicated by #, p-value < 0.05 is indicated by #, and p-value < 0.01 is indicated by ##. The significance of the sample group, PC group and NC group is indicated by *, p-value < 0.05 is indicated by *, and p-value < 0.01 is indicated by **.

[0356] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An anti-inflammatory repair composition, characterized in that: The composition comprises an extract of Anemarrhena asphodeloides obtained by fermenting Anemarrhena asphodeloides with Psoralea corylifolia.

2. A repair antioxidant composition, characterized in that: The composition comprises an extract of Anemarrhena asphodeloides obtained by fermenting Anemarrhena asphodeloides with Psoralea corylifolia.

3. The composition according to claim 2, characterized in that The composition also comprises tea extract, licorice extract and oldenlandia diffusa extract.

4. The composition according to claim 3, characterized in that The mass ratio of the Anemarrhena asphodeloides extract, the tea extract, the Glycyrrhiza glabra extract and the Hedyotis diffusa extract is 1-3: 5-15: 2-8: 1-3.

5. The composition according to claim 3, characterized in that The mass ratio of the Anemarrhena asphodeloides extract, the tea extract, the Glycyrrhiza glabra extract and the Hedyotis diffusa extract is 3:10:6:

2.

6. The composition according to claim 2, characterized in that The preparation method of the Anemarrhena Rhizoma extract is as follows: S01 strain activation: Activate the golden flower bacteria on PDA plate solid culture medium and culture at 28°C for 7 days until obvious colonies grow; S02 Symbiotic bacteria activation: Yeast was cultured in YPD medium for 24 hours and set aside; S03 Preparation of liquid seed solution: inoculate the activated bacteria into a liquid culture medium containing glucose and yeast extract, and culture in a constant temperature shaker at 28° C. and 150 rpm for 48 hours to prepare a seed solution. S04 Preliminary liquid fermentation: Add Anemarrhena powder to liquid culture medium, adjust pH to 5.5, inoculate 5% Jinhua bacteria liquid; fermentation conditions are set to 28°C, 150rpm, aerobic fermentation for 3 days; S05 Adding symbiotic bacteria in the middle stage: On the third day of liquid fermentation, add activated yeast and continue to culture for 3 to 4 days; S06 transfer to solid-state fermentation: the fermentation liquid is adsorbed on the wheat bran and placed in a constant humidity incubator for static fermentation at 25°C for 7 days; S07 obtaining the final fermentation component: mixing the whole fermentation product of step S06 with the same volume of deionized water, stirring and soaking, then centrifuging to obtain the supernatant, then concentrating under low temperature and reduced pressure, and freeze-drying to obtain the Anemarrhena asphodeloides extract.

7. The composition according to claim 5, characterized in that The components of the liquid culture medium in S04 include: Glucose 20-30g / L, peptone 5-10g / L, yeast extract 2-5g / L, ammonium nitrate 1-2g / L, magnesium sulfate heptahydrate 0.5g / L, vitamin B1 0.5g / L, gum arabic 0.05g / L, water 1L.

8. A skin care product, characterized in that: A composition comprising any one of claims 2 to 7.

9. The skin care product according to claim 8, characterized in that: The raw materials for its preparation include: Glycerin: 4 parts p-Hydroxyacetophenone: 0.3 parts Carbomer: 0.6 parts Arginine: 0.6 parts The repair antioxidant composition: 10-15 parts 1,2-Hexanediol: 0.5 parts Water was added to 100 parts.

10. The composition according to any one of claims 2 to 7, characterized in that Used in daily chemical field.

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