Itching relieving and soothing skin care lotion and preparation method thereof

Abstract

The invention relates to an itching relieving and soothing skin care lotion and a preparation method thereof. The skin care lotion is prepared from the following raw materials in parts by weight: 10-20 parts of grease, 1-3 parts of a nonionic emulsifier, 0.1-1 part of a skin feeling regulator, 0.5-2 parts of a first skin conditioner, 1-5 parts of a second skin conditioner, 0.1-1 part of an antioxidant, 1-2 parts of a humectant, 0.1-1 part of a pH regulator and 70-85 parts of water, the first skin conditioner contains a citrus peel extract and carboxymethyl chitosan, and the second skin conditioner contains hydroxyphenyl propionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, alpha-bisabolol and guaiazulene. The skin care lotion does not contain diphenhydramine hydrochloride and other substances with safety risks, is safe and mild, has remarkable itching relieving and soothing effects, and is safe and non-irritant to infants and children; during preparation, heating is little, and the activity of active substances can be reserved to the maximum extent so as to effectively ensure the itching relieving and relieving effects of the product.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care, and particularly relates to an antipruritic and soothing skin care lotion and a preparation method thereof. Background Art

[0002] Because the skin barrier of infants and children is thinner than that of adults, and their immune systems are not fully developed, they will experience more severe itching and redness and swelling when bitten by mosquitoes. Itching can cause severe discomfort to infants and children, stimulating scratching and even leading to skin damage, infection and inflammation.

[0003] Currently, most antipruritic products on the market usually use diphenhydramine hydrochloride as the main ingredient. Diphenhydramine hydrochloride is an ethanolamine antihistamine that can effectively antagonize histamine to reduce or suppress itching. However, there are risks in using diphenhydramine hydrochloride drugs for children under 2 years old. The drug may have a depressant effect on the central nervous system and has a potential risk of respiratory depression.

[0004] Therefore, how to design a safe and gentle antipruritic and soothing product is a technical problem that needs to be solved urgently in this field. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide an antipruritic and soothing composition, a skin care lotion and a preparation method thereof, aiming to overcome the above-mentioned deficiencies existing in the prior art.

[0006] The technical solution for solving the above technical problems is as follows: an antipruritic and soothing skin care lotion, which is composed of the following raw materials in parts by weight: 10-20 parts of oil, 1-3 parts of non-ionic emulsifier, 0.1-1 parts of skin feel regulator, 0.5-2 parts of first skin conditioner, 1-5 parts of second skin conditioner, 0.1-1 parts of antioxidant, 1-2 parts of moisturizer, 0.1-1 parts of pH regulator and 70-85 parts of water; wherein the first skin conditioner contains citrus peel extract and carboxymethyl deacetylated chitosan, and the second skin conditioner contains hydroxyphenyl propionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, α-bisabolol and guaiacyl.

[0007] On the basis of the above technical solutions, the present invention may also have the following further specific or more preferred options.

[0008] Specifically, the weight ratio of the citrus peel extract to carboxymethyl chitosan in the first skin conditioner is 1-2:2-5.

[0009] Carboxymethyl chitosan is a polysaccharide derived from marine organisms and has multiple biological activities. Carboxymethyl chitosan has excellent antipruritic and anti-inflammatory effects. The molecular mechanism of its antipruritic effect is currently speculated to be that carboxymethyl chitosan competes with histamine receptor H1R to reduce the itching caused by histamine. Carboxymethyl chitosan exerts a soothing effect by significantly reducing the levels of inflammatory factors IL-6 and TNF-α.

[0010] Citrus peel extract is extracted from the green peel of the citrus fruit, which is rich in flavonoids and alkaloids. In the itching pathway, after the NK1 receptor of mast cells is activated by substance P, the cell membrane "ion channel" TRPV1 opens, and the influx of calcium ions generates electrical signals, stimulating the secretion of histamine. Citrus peel extract can significantly inhibit the activation of TRPV1, prevent the influx of calcium ions, inhibit the resulting release of histamine, and relieve itching.

[0011] Specifically, the weight ratio of hydroxyphenylpropionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, α-bisabolol and guaiazulene in the second skin regulator is 5-10:10-50:10-50:10-50:1-5.

[0012] Hydroxyphenylpropionamide benzoic acid, also known as dihydrooat alkaloid, is an active ingredient extracted from oats. This ingredient can effectively antagonize substance P released by keratinocytes and nerve endings, compete with NK1 receptors on mast cells, inhibit mast cells from releasing histamine, and relieve itching. Hydroxyphenylpropionamide benzoic acid can reduce the release of IL-8 that causes atopic dermatitis. This cytokine plays a key role in the inflammatory response. At the same time, hydroxyphenylpropionamide benzoic acid can inhibit the activity of NF-κB degrading enzyme induced by TNF-α, reduce the release of inflammatory factors, and block the occurrence of cellular inflammation.

[0013] Stearyl glycyrrhizinate has excellent soothing and anti-inflammatory effects, and effectively inhibits inflammatory factors such as IL-1α, TNF-α and PEG-2. Studies have shown that glycyrrhizic acid can effectively inhibit the scratching behavior of mice caused by substance P and PAR-2 ​​agonist peptide.

[0014] Menthyl lactate can effectively activate the skin cold receptor TRPM8, and the activation of TRPM8 can indirectly affect the perception of itch. It is currently believed that the activation of TRPM8 leads to increased cold sensitivity of nerve cells, which may reduce the transmission of itch signals. At the same time, the activation of TRPM8 may also affect the release of key neuropeptides involved in the transmission of itch signals to inhibit the itch sensation.

[0015] α-Bisabolol is a natural compound with significant anti-inflammatory properties that can resist a variety of inflammatory factors. According to research, α-Bisabolol reduces the production of pro-inflammatory leukotrienes by inhibiting 5-lipoxygenase (5-LOX), which play an important role in inflammatory responses. In addition, it can also reduce the expression of pro-inflammatory factors such as IL-6, TNF-α, PGE2 and NO, thereby exerting an anti-inflammatory effect and having a soothing effect.

[0016] Specifically, the oil is any one or more of squalane, GTCC, isononyl isononanoate and isopentyl laurate.

[0017] Specifically, the nonionic emulsifier is any one or more of Seppic M68 (main ingredients are cetearyl alcohol and cetearyl glucoside), Olivem1000 (main ingredients are cetearyl olivate and sorbitan olivate), and Seppic 305 (main ingredients are polyacrylamide, C13-14 isoparaffin, and laureth-7).

[0018] Specifically, the skin feel regulator is any one or more of Carbomer 980, Carbomer 941, Polyacrylate Crosspolymer-6 and Acrylic Acid (Ester) / C10-30 Alkyl Acrylate Crosspolymer.

[0019] Preferably, the antioxidant is hydroxyacetophenone.

[0020] Specifically, the heat preservation agent is any one of 1,2-pentanediol and 1,2-hexanediol or a mixture of the two.

[0021] Specifically, the pH regulator is any one of arginine and aminomethyl propanol or a mixture of the two.

[0022] In addition, the present invention also provides a method for preparing the above-mentioned antipruritic and soothing skin care lotion, taking each raw material according to the corresponding weight parts specified in the above-mentioned antipruritic and soothing skin care lotion, and then preparing it according to the following steps:

[0023] S1. Take 1 / 5 to 1 / 3 of the total amount of water, add the first skin conditioning agent, stir and dissolve, and set aside, numbered as material A;

[0024] S2. Take 1 / 5 to 1 / 3 of the total amount of water, add a skin feel regulator and a pH regulator, stir thoroughly and swell for later use, numbered as material B;

[0025] S3. Take the remaining water, add the antioxidant, and heat until it is completely dissolved and set aside, numbered as material C;

[0026] S4. Take 1 / 5 to 2 / 5 of the total amount of oil esters, add the second skin conditioning agent, stir well to dissolve, set aside, numbered material D;

[0027] S5. Take the remaining grease, add a nonionic emulsifier, and stir until it is completely dissolved or dispersed for later use, numbered as material E;

[0028] S6. Take material C, add material E to it under stirring conditions, homogenize for 2-3 minutes, then add material A, material D and moisturizer, continue homogenizing for 3-5 minutes, and finally add material B, continue homogenizing for 10-20 minutes until the system is completely uniform.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The skin care lotion provided by the present invention contains the first and second skin conditioning agents, and the corresponding hydroxyphenylpropionamide benzoic acid, stearyl glycyrrhetinate, carboxymethyl chitosan, citrus peel extract, etc. achieve synergistic effects by inhibiting multiple targets upstream and downstream of the itching pathway, thereby achieving better antipruritic effects; stearyl glycyrrhetinate, carboxymethyl chitosan, guaiazulene and α-bisabolol, etc. also achieve strong soothing effects by inhibiting multiple inflammatory factors and antibacterial effects. Experimental studies have fully confirmed that the skin care lotion provided by the present invention has an excellent antipruritic effect. The skin care lotion is mild, safe and non-irritating, has obvious antipruritic effects, can quickly relieve itching caused by mosquito bites, and can effectively eliminate rashes.

[0031] The raw materials of this skin care lotion do not contain substances with safety risks such as diphenhydramine hydrochloride. It is a safe, mild and anti-itching and soothing product with significant anti-itching and soothing effects. This lotion is safe and non-irritating to infants and children, and has the effect of relieving skin itching and reducing the redness and swelling of mosquito bites.

[0032] In addition, most of the steps of the preparation method provided by the present invention do not require heating, which greatly reduces the energy loss in the production process and can retain the activity of the active substances to the maximum extent, thereby effectively ensuring the high quality of the prepared skin care lotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 A bar graph showing the inhibition rate of hyaluronidase by the antipruritic and soothing skin care lotions prepared in various embodiments of the present invention during the soothing efficacy test;

[0034] Figure 2 A line chart showing the mean change in itching sensation of the volunteers after application of the antipruritic and soothing skin care lotions prepared in various embodiments of the present invention in the antipruritic test. DETAILED DESCRIPTION

[0035] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0036] To avoid redundant description, the drugs used in the following examples are all commercially available products unless otherwise specified, and the methods used are all conventional methods in the art unless otherwise specified.

[0037] The first skin conditioning agent used in the following examples is a mixture of citrus peel extract and carboxymethyl chitosan in a weight ratio of 1-2:2-5, preferably 1:3; the second skin conditioning agent is a mixture of hydroxyphenylpropionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, α-bisabolol, and guaiazulene in a weight ratio of 5-10:10-50:10-50:10-50:1-5, preferably 5:20:18:26:4. The first and second skin conditioning agents used in the following examples and comparative examples preferably use conditioning agents corresponding to the optimal weight ratio.

[0038] The raw materials and their corresponding weight parts in the skin care emulsions corresponding to Examples 1 to 3 and Comparative Examples 1 to 3 are shown in Table 1 below:

[0039] Table 1. Raw materials and corresponding weight parts used in preparing corresponding emulsions in various embodiments and comparative examples

[0040] Element Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Carbomer 980 0.4 0.4 0.4 0.4 0.4 0.4 olivem1000 2.5 2.5 2.5 2.5 2.5 2.5 Squalane 16 16 16 16 16 16 p-Hydroxyacetophenone 0.5 0.5 0.5 0.5 0.5 0.5 1,2-Hexanediol 1.0 1.0 1.0 1.0 1.0 1.0 Arginine 0.5 0.5 0.5 0.5 0.5 0.5 First Skin Conditioner 1.2 1.5 1.8 3 / / Secondary Skin Conditioner 1.8 1.5 1.2 / 3 / water to 100 to 100 to 100 to 100 to 100 To 100

[0041] Example 1 to Example 3

[0042] An antipruritic and soothing skin care lotion is prepared by the following method. The raw materials of each example in the preparation are taken according to the amounts specified in Table 1. The specific steps are as follows:

[0043] S1. Take about 1 / 4 of the total amount of water, add the first skin conditioning agent, stir and dissolve, and set aside, numbered as material A;

[0044] S2. Take about 1 / 4 of the total amount of water, add a skin feel regulator (carbomer 980) and a pH regulator (arginine), stir thoroughly and swell for later use, and number it as material B;

[0045] S3. Take the remaining water, add antioxidant (p-hydroxyacetophenone), heat until it is completely dissolved and set aside, numbered as material C;

[0046] S4. Take about 1 / 3 of the total amount of oil ester (squalane), add the second skin conditioning agent, stir thoroughly to dissolve, and set aside, numbered as material D;

[0047] S5. Take the remaining ester (squalane), add a nonionic emulsifier (Sepic 305), stir until it is completely dissolved or dispersed and set aside, numbered as material E;

[0048] S6. Take material C, add material E to it under stirring, homogenize for 2-3 minutes, then add material A and material D and humectant (1,2-hexanediol), continue homogenizing for 3-5 minutes, and finally add material B, continue homogenizing for 10-20 minutes until the system is completely uniform.

[0049] Comparative Example 1

[0050] A skin care lotion, in which the raw materials are taken in the amounts specified in Table 1, the specific steps are as follows and the preparation method is as follows:

[0051] (1) Take about 1 / 4 of the total amount of water, add the first skin conditioning agent, stir and dissolve for later use, and number it as material A.

[0052] (2) Take about 1 / 4 of the total amount of water, add Carbomer 980, stir and swell, and then add arginine, which is numbered as material B.

[0053] (3) Take the remaining water, add p-hydroxyacetophenone, and heat until it is completely dissolved for later use, which is numbered as material C.

[0054] (4) Add Seppic 305 to squalane and stir until completely dissolved. Set aside and number it as material D.

[0055] (5) Take material C, stir it, add material A, material D and 1,2-pentanediol, and homogenize for 3-5 minutes. Then add material B into the system, and continue homogenizing for 10-20 minutes until the system is completely uniform.

[0056] Comparative Example 2

[0057] A skin care lotion, in which the raw materials are taken in the amounts specified in Table 1, the specific steps are as follows and the preparation method is as follows:

[0058] (1) Take about 1 / 4 of the total amount of water, add Carbomer 980, stir and swell, and then add arginine, which is numbered as material A.

[0059] (2) Take the remaining water, add p-hydroxyacetophenone, and heat until it is completely dissolved for later use, which is numbered as material B.

[0060] (3) Take about 1 / 3 of the total amount of squalane, add the second skin conditioning agent, stir thoroughly to dissolve, and set aside. This is numbered as material C.

[0061] (4) Take the remaining squalane, add Seppic 305, stir until completely dissolved, and set aside. This is numbered as material D.

[0062] (5) Take material B at room temperature, stir it, and add material C, material D and 1,2-pentanediol into it, and homogenize for 3-5 minutes. Then add material A into the system, and continue homogenizing for 10-20 minutes until the system is completely uniform.

[0063] Comparative Example 3

[0064] A skin care lotion, in which the raw materials are taken in the amounts specified in Table 1, the specific steps are as follows and the preparation method is as follows:

[0065] (1) Take about 1 / 4 of the total amount of water, add Carbomer 980, stir and swell, and then add arginine, which is numbered as material A.

[0066] (2) Take the remaining water, add p-hydroxyacetophenone, and heat until it is completely dissolved for later use, which is numbered as material B.

[0067] (3) Take squalane, add Seppic 305, stir until completely dissolved, and set aside. This is numbered as material C.

[0068] (4) Take material B at room temperature, stir it, add material C and 1,2-pentanediol, and homogenize for 3-5 minutes. Then add material A into the system, and continue homogenizing for 10-20 minutes until the system is completely uniform.

[0069] Test Example 1

[0070] Product stability testing

[0071] (1) Heat resistance test: Take a small amount of samples and place them in a constant temperature box at 50℃ and 45℃. Take samples every 7 days and observe them after returning to room temperature for 3 months.

[0072] (2) Cold resistance test: Take a small amount of samples and place them in a -20℃ refrigerator. Take samples every 7 days and observe them after returning to room temperature for 3 months.

[0073] (3) Centrifugation test: Take a small amount of sample and centrifuge it at 3000 r / min for 30 min and observe the state.

[0074] Conclusion: Examples 1-3 and Comparative Examples 1-3 all passed the heat resistance test, cold resistance test and centrifugation test, and no stratification or deterioration occurred during the test, indicating that the antipruritic and soothing skin care lotion of the present invention has good stability.

[0075] Test Example 2

[0076] Soothing effect test

[0077] Hyaluronidase is an enzyme that can hydrolyze hyaluronic acid and is a participant in type I allergic reactions. Hyaluronidase is strongly associated with inflammation and allergies. The in vitro inhibition test of hyaluronidase can reflect the anti-inflammatory, anti-allergic and soothing effects of the product to a certain extent.

[0078] Hyaluronidase catalyzes the reaction with the substrate. After adding the active substance, the absorbance changes. The hyaluronidase activity inhibition rate is calculated by the absorbance change. The color development degree of the reaction solution is linearly related to the sample concentration. From this, the ability of the test sample to inhibit the activity of hyaluronidase can be evaluated, which can be used as a basis for evaluating the soothing effect of the sample.

[0079] The antiallergic activity was determined using a modified version of the Morgan-Elson method, as follows: 100 μL of a mixed buffer of 0.2 M sodium acetate and 0.15 M NaCl was added to tubes A and B, 100 μL of sample solution was added to tubes C and D, 50 μL of 500 U / mL hyaluronidase solution was added to tubes A and C, and 50 μL of mixed buffer was added to tubes B and D. After incubation at 37°C for 20 min, 20 μL of 2.5 mol / L CaCl was added to the system. 2 , incubate at 37°C for 20 min. Then add 50 μL of 3 mg / mL hyaluronic acid solution, 100 μL of mixed buffer, 250 μL of deionized water to each test tube and incubate at 37°C for 40 min. Then stop the enzymatic reaction by adding 110 μL of alkaline borate solution and then heating in a boiling water bath for 5 min. Then place the test tube in ice water for 20 min, then add 1.5 mL of p-dimethylaminobenzaldehyde solution, and incubate the test tube at 37°C for 20 min. Measure the absorbance at 585 nm. The example samples in the above tests are all 1% aqueous solutions. The hyaluronidase inhibition rate is calculated using the following formula:

[0080] Inhibition rate (%) = [(AB) - (CD)] / (AB) × 100

[0081] Where A is the control absorbance; B is the control blank absorbance; C is the sample absorbance; and D is the sample blank absorbance.

[0082] The inhibition rate of hyaluronidase by the antipruritic skin care lotion corresponding to each embodiment is as follows: Figure 1 As shown, combined with the blank control situation, it can be seen that the antipruritic skin care lotion provided by the present invention has a strong hyaluronidase inhibitory effect and has a soothing effect.

[0083] Test Example 3

[0084] Itch relief test

[0085] This anti-itch test mainly targets the itching reaction caused by mosquito bites, and adopts the form of consumer trials and questionnaire surveys. The rules are as follows:

[0086] First, the intensity of the itching is divided. 0 points: no itching. 1-2 points: slight itching, almost not noticeable, no need to scratch. 3-4 points: mild itching, occasionally need to scratch gently, but can be easily ignored. 5 points: moderate itching, obvious itching, need to scratch occasionally to relieve. 6-7 points: strong itching, the itching is more obvious, need to scratch frequently, but can still control not to scratch the skin. 8-9 points: severe itching, the itching is very strong and almost can not be ignored, frequent and hard scratching, there is a risk of scratching the skin. 10 points: extreme itching, the itching is unbearable, continuous scratching is likely to cause skin damage.

[0087] 60 volunteers under 35 years old who are susceptible to mosquito bites were recruited and divided into 6 groups, each with 10 people. The samples tried in each group corresponded to Examples 1 to 3 and Comparative Examples 1 to 3, respectively. The subjective itch score data of the volunteers at different time points after applying the antipruritic soothing lotion after being bitten by mosquitoes were collected and sorted, and the itch mean data are shown in the following table.

[0088]

[0089]

[0090] Figure 2 The above table shows the statistical chart of the change of itching sensation. It can be seen from the analysis that compared with Comparative Example 3 (blank cream), the other groups all have obvious antipruritic effects. The effects of Examples 1 to 3 are similar, and compared with Comparative Example 1 (the first skin conditioner is increased and the second skin conditioner is missing) and Comparative Example 2 (the second skin conditioner is increased and the first skin conditioner is missing), the antipruritic effect within 2 to 5 minutes after application is more significant, and the immediate antipruritic effect is better.

[0091] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An antipruritic and soothing skin care lotion, characterized in that: The invention is composed of the following raw materials in parts by weight: 10-20 parts of oil, 1-3 parts of nonionic emulsifier, 0.1-1 parts of skin feel regulator, 0.5-2 parts of first skin conditioner, 1-5 parts of second skin conditioner, 0.1-1 parts of antioxidant, 1-2 parts of moisturizer, 0.1-1 parts of pH regulator and 70-85 parts of water; wherein the first skin conditioner contains citrus peel extract and carboxymethyl deacetylated chitosan, and the second skin conditioner contains hydroxyphenyl propionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, α-bisabolol and guaiacyl.

2. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The weight ratio of the citrus peel extract to carboxymethyl chitosan in the first skin conditioner is 1-2:2-5.

3. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The weight ratio of hydroxyphenylpropionamide benzoic acid, stearyl glycyrrhetinate, menthol lactate, α-bisabolol and guaiazulene in the second skin regulator is 5-10:10-50:10-50:10-50:1-5.

4. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The oil is any one or more of squalane, GTCC, isononyl isononanoate and isopentyl laurate.

5. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The nonionic emulsifier is any one or more of Seppic M68, OliveM1000 and Seppic 305.

6. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The skin feel regulator is any one or more of Carbomer 980, Carbomer 941, Polyacrylate Crosspolymer-6 and Acrylic Acid (Ester) / C10-30 Alkyl Acrylate Crosspolymer.

7. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The antioxidant is hydroxyacetophenone.

8. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The moisturizing agent is any one of 1,2-pentanediol and 1,2-hexanediol or a mixture of the two.

9. The antipruritic and soothing skin care lotion according to claim 1, characterized in that: The pH regulator is any one of arginine and aminomethyl propanol or a mixture of the two.

10. A method for preparing an antipruritic and soothing skin care lotion, characterized in that: Take each raw material according to the corresponding weight parts specified in the antipruritic soothing skin care lotion according to any one of claims 1 to 9, and then process it according to the following steps: S1. Take 1 / 5 to 1 / 3 of the total amount of water, add the first skin conditioning agent, stir and dissolve, and set aside, numbered as material A; S2. Then take 1 / 5 to 1 / 3 of the total amount of water, add a skin feel regulator and a pH regulator, stir thoroughly and swell for later use, numbered as material B; S3. Take the remaining water, add the antioxidant, and heat until it is completely dissolved and set aside, numbered as material C; S4. Take 1 / 5 to 2 / 5 of the total amount of oil ester, add the second skin conditioning agent, stir well to dissolve, set aside, numbered material D; S5. Take the remaining grease, add a nonionic emulsifier, and stir until it is completely dissolved or dispersed for later use, numbered as material E; S6. Take material C, add material E to it under stirring conditions, homogenize for 2-3 minutes, then add material A, material D and moisturizer, continue homogenizing for 3-5 minutes, and finally add material B, continue homogenizing for 10-20 minutes until the system is completely uniform.

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