A soothing and repairing composition and its application

Abstract

The present invention provides a soothing and repairing composition and application thereof, and belongs to the technical field of cosmetics. The present invention mainly provides a soothing and repairing composition, which comprises the following components by weight: 0.05-3.0 parts of sodium DNA, 1.0-15.0 parts of agarwood extract and 1.0-15.0 parts of juniper fruit extract. The present invention firstly finds that after sodium DNA, agarwood extract and juniper fruit extract are compounded, they have significant synergistic effects in soothing and repairing, and have good application prospects in various types of cosmetics.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a soothing and repairing composition and application thereof. Background Art

[0002] Sensitive skin (SS) refers specifically to a highly reactive state of the skin under physiological or pathological conditions, mainly occurring on the face. Clinically, the skin is prone to subjective symptoms such as burning, stinging, itching, and tightness when stimulated by physical, chemical, mental and other factors, with or without objective signs such as erythema, scaling, and capillary dilation. The pathogenesis behind sensitive skin is a complex process, the main cause of which is impaired skin barrier function. Impaired skin barrier function accelerates transepidermal water loss and loss of skin nutrients, and also enhances the penetration of irritants or allergens, resulting in insufficient protection of nerve fibers in the epidermis, exposing deeper layers of the skin to microorganisms and external stimuli, thereby inducing local inflammation of the skin, with symptoms such as redness, itching, burning and stinging. Therefore, the care of sensitive skin must repair the damaged skin barrier and reduce the release of skin inflammatory cytokines.

[0003] Soothing skin sensation and reducing skin inflammation can improve sensitive skin and reduce secondary damage to the skin barrier caused by scratching. Current studies have shown that many cosmetic ingredients can effectively inhibit skin inflammatory responses, reduce the expression of inflammatory factors, and thus relieve symptoms such as skin redness and dryness to achieve a soothing effect. At the same time, moisturizing should also be done to prevent dry skin and peeling. When the skin barrier is damaged, adverse external factors stimulate skin keratinocytes to produce pro-inflammatory factors such as TNF-α and IL-6. When the ducts rupture, the release will cause a strong local reaction of the innate immune system, resulting in varying degrees of itching, burning, tingling, and even erythema, causing skin discomfort. When the expression of barrier-related proteins such as filaggrin (FLG) and loricrin (LOR) in the skin is downregulated, it will also lead to abnormal formation of the stratum corneum, thereby affecting the skin barrier function.

[0004] Surfactants are the main cleaning ingredients commonly used in personal care cleaning products such as facial cleansers, shampoos, and shower gels. In rinse-off personal care cleaning products, although the surfactants are in contact with human skin for a short time, the surfactants’ penetration into the skin, the interaction with intercellular lipids leading to a reduction in lipids or changes in the composition ratio, and the binding, swelling, and denaturation of proteins, will not only affect the barrier function of the stratum corneum, but also affect the growth and differentiation regulation of keratinocytes, thereby affecting the formation of a healthy stratum corneum, destroying the skin barrier, and causing irritation. The transdermal penetration ability of surfactants is considered to be one of the causes of various skin inflammations. The penetration of surfactants changes the original structural state of the skin and the compatibility between adjacent molecules, thereby causing contact dermatitis and dermal dermatitis, causing skin irritation and even allergic reactions, resulting in erythema and edema on the skin. The irritation of surfactants to the skin and mucous membranes is most severe for cationic surfactants, followed by anionic surfactants, and the least for nonionic and zwitterionic surfactants.

[0005] Currently, there is little research on reducing the irritation of soap-based cleansers or amino acid-based cleansers, or the current ingredients cannot effectively reduce their irritation. Therefore, it is necessary to develop ingredients with simple ingredients, mild and non-irritating, and good soothing and repairing effects, and apply them to products to reduce the irritation of cleansing products to the skin. Summary of the invention

[0006] In view of the above problems, the present invention provides a soothing and repairing composition and application thereof.

[0007] The present invention is the first to discover a composition formed by compounding sodium DNA, agarwood extract and juniper fruit extract, which has good soothing and repairing effects, can be used to improve the irritation problem of cleansing products in cosmetics, and has good application prospects in cosmetics.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A soothing and repairing composition comprises the following components by weight: 0.05-3.0 parts of sodium DNA, 1.0-15.0 parts of agarwood extract and 1.0-15.0 parts of juniper fruit extract.

[0010] Preferably, according to the present invention, the soothing and repairing composition comprises the following components by weight: 0.1 to 2.0 parts of sodium DNA, 5.0 to 10.0 parts of agarwood extract and 5.0 to 10.0 parts of juniper fruit extract.

[0011] Further preferably, the soothing and repairing composition comprises the following components by weight: 1.0 part of sodium DNA, 8.0 parts of agarwood extract and 8.0 parts of juniper berry extract.

[0012] Preferably, according to the present invention, the agarwood extract in the soothing and repairing composition is a commercially available product or is prepared by the following method, and the specific steps are as follows:

[0013] The agarwood is crushed to a particle size of 40 to 80 meshes, the crushed agarwood is mixed with an ethanol solution with a volume fraction of 65 to 95% in a mass ratio of 1: (20 to 30), and then heated reflux extraction is performed, the reflux temperature is 55 to 65° C., and the extraction time is 110 to 130 min; after the extraction is completed and cooled, centrifugation is performed at a centrifugal speed of 5000 to 6000 rpm for 10 to 15 min to obtain a supernatant; the supernatant is reduced pressure concentrated to an extract under a pressure of 0.04 to 0.1 MPa and a temperature of 40 to 60° C., and the obtained concentrate is dispersed and dissolved with butanediol, the mass ratio of the concentrate to butanediol is 1: (55 to 70), and after dissolution, it is filtered to obtain the filtrate, which is the agarwood extract.

[0014] More preferably, after dissolution, filtration is performed using a 0.8 μm filter membrane, and the obtained filtrate is the agarwood extract.

[0015] Further preferably, the agarwood is crushed to a particle size of 40 to 80 meshes, the crushed agarwood is mixed with an ethanol solution with a volume fraction of 80 to 95% in a mass ratio of 1:25, and then heated to reflux for extraction, the reflux temperature is 55 to 65°C, and the extraction time is 120 min; after the extraction is completed and cooled, centrifuge at a centrifugal speed of 5500rpm for 10 min to obtain a supernatant; the supernatant is concentrated under reduced pressure at 0.08MPa and 60°C to an extract, and the obtained concentrate is dispersed and dissolved with butanediol, the mass ratio of the concentrate to butanediol is 1:60, and after dissolution, it is filtered with a 0.8μm filter membrane to obtain the filtrate, which is the agarwood extract.

[0016] Preferably, according to the present invention, the juniper berry extract in the soothing and repairing composition is a commercially available product or is prepared by the following method, and the specific steps are as follows:

[0017] The dried juniper berries are crushed and passed through a 40-100 mesh sieve, the crushed juniper berries are mixed with an ethanol solution with a volume fraction of 60-95% in a mass ratio of 1:(20-40), and the mixture is extracted 2-4 times in a water bath at 65-75°C, each extraction lasting 60-120 minutes. After the extraction is completed, the mixture is centrifuged at a centrifugal speed of 5000-6000 rpm for 10-15 minutes, the supernatants are combined, and the mixture is concentrated under reduced pressure to an extract, 0.5-1.0 times the mass of butanediol is added, the mixture is stirred evenly, and the mixture is filtered through a filter membrane, and the obtained filtrate is the juniper berry extract.

[0018] More preferably, filtration is performed using a 0.8 μm filter membrane, and the obtained filtrate is the Juniperus chinensis fruit extract.

[0019] Further preferably, the dried juniper fruit is crushed and passed through a 100-mesh sieve, the crushed juniper fruit is mixed with a 60-95% ethanol solution in a mass ratio of 1:30, and extracted three times in a water bath at 65°C, each time for 1 hour. After the extraction is completed, centrifuge at 5500rpm for 15 minutes, combine the supernatants, concentrate under reduced pressure to an extract, add 0.6 times the mass of butanediol, stir evenly, and filter with a 0.8μm filter membrane to obtain the filtrate, which is the juniper fruit extract.

[0020] The preparation method of the soothing and repairing composition comprises the following steps:

[0021] According to weight parts, corresponding weight parts of sodium DNA, agarwood extract, and juniper fruit extract are mixed with water, and the amount of water is supplemented to 100 parts to obtain a soothing and repairing composition.

[0022] Preferably according to the present invention, in the above preparation method, the water is deionized water.

[0023] Application of the soothing and repairing composition in the preparation of cosmetics.

[0024] Preferably, according to the present invention, the soothing and repairing composition is used in the preparation of soothing and repairing skin care cosmetics.

[0025] A cosmetic comprising the soothing and repairing composition.

[0026] Preferably according to the present invention, the cosmetics are soothing and repairing skin care cosmetics.

[0027] Further preferably, the types of soothing and repairing skin care cosmetics include cleansing milk / cream, essence water, essence liquid, facial mask, essence lotion, and essence cream.

[0028] Preferably according to the present invention, based on the total mass of the soothing and repairing skin care cosmetic, the cosmetic contains 0.5 to 30% of the soothing and repairing composition.

[0029] Further preferably, based on the total mass of the soothing and repairing skin care cosmetic, the cosmetic contains 2 to 20% of the soothing and repairing composition.

[0030] Preferably according to the present invention, the cosmetics include facial cleansers, lotions, creams, masks, shampoos and hair care products, and body care products.

[0031] The beneficial technical effects of the present invention include at least the following:

[0032] 1. The composition provided by the present invention can significantly promote the expression of FLG and LOR, effectively inhibit the activity of IL-6 and TNF-α inflammatory factors, thereby achieving an effective soothing and repairing effect; the inventors found through hyaluronidase inhibition test that the composition provided by the present invention has significant moisturizing ability; in addition, the inventors found through facial image analyzer that the composition provided by the present invention can significantly reduce the redness value of the face, and the soothing effect is obvious. The present invention first discovered that after the combination of DNA sodium, agarwood extract, and European juniper fruit extract, it has a significant synergistic effect in soothing and repairing, and has a good application prospect in various types of cosmetics.

[0033] 2. The composition provided by the present invention is used for facial cleansing products. The chicken embryo chorioallantoic membrane test found that it can significantly reduce the irritation of facial cleansing products and has a good soothing effect. The inventors found through foam experiments that the composition provided by the present invention can be used for facial cleansing products and can also significantly improve the foaming ability of facial cleansing products, making the product foam richer. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a diagram showing the test results of a facial image analyzer before and after applying the composition of Example 2;

[0035] In the figure: the left picture is D0, the right picture is D 14 sky.

[0036] Figure 2 This is a graph showing the test results of the chicken embryo chorioallantoic membrane test of Example 4;

[0037] In the figure, the left picture is before adding the sample, and the right picture is after adding the sample.

[0038] Figure 3 This is a graph showing the test results of the chicken embryo chorioallantoic membrane test of Comparative Example 7;

[0039] In the figure, the left picture is before adding the sample, and the right picture is after adding the sample. DETAILED DESCRIPTION

[0040] The present invention is further described below by examples, but the present invention is not limited to the scope of the embodiments. Based on the embodiments in the present invention, any changes to the present invention by those skilled in the art without creative work are all within the scope of the present invention. Meanwhile, in the embodiments of the present invention, unless otherwise specified, all preparation raw materials are commercially available products well known to those skilled in the art.

[0041] The "purified water" mentioned below refers to deionized water.

[0042] The compositions corresponding to Examples 1 to 3 and Comparative Examples 1 to 6 are:

[0043] The specific scheme is shown in Table 1 below, calculated by weight. The preparation method is as follows: DNA sodium, agarwood extract, and juniper fruit extract are mixed together and dissolved in purified water.

[0044] Table 1: List of ingredients of Examples 1 to 3 and Comparative Examples 1 to 6

[0045]

[0046] In the above table, the DNA sodium raw material comes from Runhui Biotechnology (Weihai) Co., Ltd.

[0047] The preparation method of agarwood extract comprises the following specific steps:

[0048] The agarwood is crushed to a particle size of 60 mesh, and the crushed agarwood is mixed with an ethanol solution with a volume fraction of 80% in a mass ratio of 1:25, and then heated to reflux extraction, the reflux temperature is 65°C, and the reflux extraction is 120 minutes; after the extraction is completed and cooled, centrifuge at a centrifugal speed of 5500rpm for 10 minutes to obtain a supernatant; the supernatant is concentrated under reduced pressure at 0.08MPa and 60°C to an extract, and the obtained concentrate is dispersed and dissolved with butanediol, the mass ratio of the concentrate to butanediol is 1:60, and after dissolution, it is filtered with a 0.8μm filter membrane to remove insoluble substances, and the obtained filtrate is the agarwood extract.

[0049] The preparation method of the juniper fruit extract comprises the following specific steps:

[0050] The method for obtaining the juniper fruit extract comprises the following steps: crushing dried juniper fruits, passing through a 100-mesh sieve, mixing the crushed juniper fruits with an ethanol solution having a volume fraction of 80% in a mass ratio of 1:30, extracting three times in a 65° C. water bath, each time for 1 hour, centrifuging at 5500 rpm for 15 minutes after the extraction, combining the supernatants, concentrating under reduced pressure to obtain an extract, adding 0.6 times the mass of butanediol, stirring evenly, filtering with a 0.8 μm filter membrane, and obtaining the filtrate to obtain the juniper fruit extract.

[0051] Example 4: Cleansing liquid

[0052] The soothing and repairing composition is used to prepare a facial cleanser product containing the soothing and repairing composition. The formula and raw material sources are shown in Table 2.

[0053] Table 2: Facial cleanser product formula

[0054]

[0055] Preparation method of cleansing liquid:

[0056] S1: Add the raw materials in phase A respectively, heat to 80-85℃ and stir to dissolve;

[0057] S2: Then add the raw materials in phase B respectively, stir and disperse to dissolve evenly;

[0058] S3: When the temperature drops to 65-70°C, add phase C, stir to dissolve, and start cooling after dissolution;

[0059] S4: When the temperature drops to 40-45°C, add the D phase ingredients, stir to dissolve and disperse evenly, and the cleansing liquid product is obtained.

[0060] Comparative Example 7: Cleansing Liquid

[0061] The preparation method of Comparative Example 7 is the same as that of Example 4, except that the soothing and repairing composition (Example 2) is not added to Comparative Example 7.

[0062] Comparative Example 8: Commercially available 100Xinminshu.

[0063] Comparative Example 9: Commercially available ectoine.

[0064] Test Example 1: In vitro repair efficacy test

[0065] This test uses a 3D epidermal skin model For the test model. Preparation of 0.1% SLS working solution: 1 mL of 0.2% SLS solution was taken and added to 1 mL of PBS to prepare 0.1% SLS working solution; Preparation of working solution of positive control group (WY14643, Merck): 10 μL of 30 mM WY14643 mother solution was taken and dissolved in 6 mL of culture medium (EpiGrowth culture medium) to prepare 50 μM WY14643.

[0066] According to the test grouping in Table 3, the model was transferred to a 6-well plate (0.9 mL EpiGrowth culture medium was added in advance), and the test group number was marked on the 6-well plate. 25 μL of 0.1% SLS solution was added to the surface of the negative control group (NC), the positive control group (PC), Examples 1 to 3, and Comparative Examples 1 to 6, and incubated for 30 minutes. After the incubation, 12.5 μL of WY14643 solution was added to the surface of the positive control group model, and 12.5 μL of the sample to be tested (the test sample of Examples 1 to 3 and Comparative Examples 1 to 6) was added to the surface of the sample test group model. After evenly coating, it was placed in a CO2 incubator (37°C, 5% CO2) and incubated for 24 hours. After the incubation, the test substance remaining on the surface of the model was cleaned with a sterile PBS solution, and the residual liquid inside and outside the model was wiped off with a sterile cotton swab.

[0067] Immunofluorescence test: Take the model for detection, cut off the model, fix it with 4% paraformaldehyde, and after fixation for 24 hours, perform immunofluorescence detection of FLG and LOR content, take pictures under a microscope, and collect and analyze the pictures.

[0068] Table 3: Experimental group settings

[0069]

[0070] The results of the immunofluorescence test are shown in Tables 4 and 5, respectively.

[0071] Table 4 Summary of FLG immunofluorescence analysis results

[0072]

[0073]

[0074] Note: The integrated optical density (IOD) reflects the content of FLG. When the t-test method was used for statistical analysis, compared with the BC group, the significance was indicated by #, and P-value < 0.01 was indicated by ##; compared with the NC group, the significance was indicated by *, 0.01 < P-value < 0.05 was indicated by *, and P-value < 0.01 was indicated by **.

[0075] As can be seen from Table 4, compared with the BC group, the FLG content of the NC group decreased significantly, indicating that the stimulation conditions of this test are effective. Compared with the NC group, the FLG content of the PC group increased significantly, indicating that the positive control of this test is effective. Compared with the NC group, the FLG content of Examples 1 to 3 and Comparative Examples 3 to 6 increased significantly, indicating that it has a certain repair effect. Compared with Comparative Examples 3 to 6, the FLG content of Examples 1 to 3 increased by more than 44%, while in Comparative Examples 1 to 6, due to the lack of one or two of the raw material components, the FLG increase of the prepared composition was less than 44%, which shows that the composition prepared by DNA sodium, agarwood extract and European juniper fruit extract in the present invention has a better repair effect. Compared with Examples 1 to 3, the FLG content of Example 2 increased the most, which shows that Example 2 is the best combination.

[0076] Table 5 Summary of LOR immunofluorescence analysis results

[0077]

[0078]

[0079] Note: The integrated optical density (IOD) reflects the content of LOR. When the t-test method is used for statistical analysis, compared with the BC group, the significance is indicated by #, P-value < 0.05 is indicated by #, and P-value < 0.01 is indicated by ##; compared with the NC group, the significance is indicated by *, 0.01 < P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0080] As can be seen from Table 5, compared with the BC group, the LOR content of the NC group decreased significantly, indicating that the stimulation conditions of this test are effective. Compared with the NC group, the LOR content of the PC group increased significantly, indicating that the positive control of this test is effective. Compared with the NC group, the LOR content of Examples 1 to 3 and Comparative Examples 3 to 6 increased significantly, indicating that they have certain repair effects. Compared with Comparative Examples 3 to 6, the LOR content of Examples 1 to 3 increased by more than 97%, while in Comparative Examples 1 to 6, due to the lack of one or two of the raw material components, the LOR content of the prepared compositions increased by less than 70%, which shows that the composition prepared by DNA sodium, agarwood extract and European juniper fruit extract in the present invention has a good soothing and repairing effect. Compared with Examples 1 to 3, the LOR content of Example 2 increased the most, which shows that Example 2 is the best combination.

[0081] Experimental Example 2: In vitro inhibition of the activity of pro-inflammatory factors TNF-α and IL-6

[0082] LPS was used to induce macrophages RAW264.7, and the expression levels of TNF-α and IL-6 were detected by ELISA to evaluate the ability of the test substances to inhibit the expression of related cell pathway factors. See Table 6 for the test scheme.

[0083] Table 6: Test plan

[0084]

[0085]

[0086] Note: The samples were diluted to 1 mg / mL using deionized water.

[0087] The test results are shown in Tables 7 and 8.

[0088] Table 7: Summary of TNF-α content results

[0089]

[0090] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control; * indicates p<0.01 compared with negative control <p<0.05。)

[0091] As can be seen from Table 7, compared with the BC group, the TNF-α content of the NC group increased significantly, indicating that the stimulation conditions of this test are effective. Compared with the NC group, the TNF-α content of the PC group decreased significantly, indicating that the positive control of this test is effective. Compared with the NC group, the TNF-α content of Examples 1 to 3 and Comparative Examples 1 to 6 decreased significantly, indicating that they all have a certain soothing effect. Compared with Comparative Examples 1 to 6, the TNF-α content of the composition prepared by Examples 1 to 3 was significantly reduced by more than 40%, while in Comparative Examples 1 to 6, due to the lack of one or two of the raw material components, the TNF-α content of the prepared composition was reduced by less than 40%, which shows that the composition prepared by DNA sodium, agarwood extract and European juniper fruit extract in the present invention has a better soothing and repairing effect. In particular, the composition described in Example 2 has the largest reduction in TNF-α content and the best soothing effect.

[0092] Table 8: Summary of IL-6 content results

[0093]

[0094]

[0095] (Note: ## indicates p<0.01 compared with blank control; ** indicates p<0.01 compared with negative control; * indicates p<0.01 compared with negative control <p<0.05。)

[0096] As can be seen from Table 8, compared with the BC group, the IL-6 content of the NC group increased significantly, indicating that the stimulation conditions of this test are effective. Compared with the NC group, the IL-6 content of the PC group decreased significantly, indicating that the positive control of this test is effective. Compared with the NC group, the IL-6 content of Examples 1 to 3 and Comparative Examples 1 to 6 decreased significantly, indicating that they have a certain soothing effect. Compared with Comparative Examples 1 to 6, the IL-6 content of the composition prepared by Examples 1 to 3 was significantly reduced by more than 47%, while in Comparative Examples 1 to 6, due to the lack of one or two of the raw material components, the TNF-α content of the prepared composition was reduced by less than 47%, which shows that the composition prepared by DNA sodium, agarwood extract and European juniper fruit extract in the present invention has a better soothing and repairing effect. In particular, the composition described in Example 2 has the largest reduction in IL-6 content and the best soothing effect.

[0097] Test Example 3: Hyaluronidase Activity Inhibition Experiment

[0098] The specific test method is as follows:

[0099] (1) Take 0.02 mL of 0.25 mmol / L CaCl2 solution and 0.1 mL of hyaluronidase solution and incubate at 37°C for 20 min;

[0100] (2) Example 2 and Comparative Examples 8 and 9 were added respectively, and the mixture was incubated at 37° C. for 20 min;

[0101] (3) Add 0.1 mL of sodium hyaluronate solution, incubate at 37°C for 30 min, and place at room temperature for 5 min;

[0102] (4) Add 0.02 mL of 0.4 mol / L NaOH solution and 0.1 mL of acetylacetone solution, heat in a boiling water bath for 15 min, immediately cool with ice water for 5 min, add 0.2 mL of Ehrlich reagent, and dilute with 0.6 mL of anhydrous ethanol. Allow to develop color for 20 min, and measure the absorbance at 510 nm using a multifunctional microplate reader.

[0103] (5) Calculation formula for the inhibition rate of hyaluronidase by the sample:

[0104] Hyaluronidase inhibition rate (%) = [(A1-A2)-(B1-B2)] / (A1-A2)×100%

[0105] Among them, A1 is the absorbance value of the reference solution; A2 is the absorbance value of the reference blank solution, in which the hyaluronidase and sodium hyaluronate solution are replaced by acetate buffer solution; B1 is the absorbance value of the sample solution; B2 is the absorbance value of the sample blank solution, in which the hyaluronidase and sodium hyaluronate solution are replaced by acetate buffer solution.

[0106] The test results are shown in Table 9.

[0107] Table 9 Inhibition rate of hyaluronidase of the tested samples

[0108]

[0109] As can be seen from the results in Table 9, the inhibition rate of the composition of Example 2 on hyaluronidase is comparable to that of Comparative Examples 8 and 9. Hyaluronic acid is essential for the moisturizing effect of the skin, and it can lock in moisture and keep the skin hydrated. The composition of Example 2 can effectively inhibit the activity of hyaluronidase, reduce the decomposition of hyaluronic acid, maintain the hyaluronic acid content in the skin at a high level, play a role in positively regulating the hyaluronic acid content in the skin, and provide continuous moisturizing for the skin.

[0110] Test example 4:

[0111] 30 volunteers were recruited for the test. Each volunteer was screened by lactate test and met the volunteer inclusion criteria.

[0112] Experimental samples and usage: Apply the composition of Example 2 evenly on the face after cleansing every morning and evening.

[0113] Test process method:

[0114] Day 1 (D0)

[0115] ① Registration of subject information, education and training of researchers, distribution of research plans, promotion of research introduction, informed consent, follow-up of subjects during the research, improvement of subject compliance, and treatment measures for adverse drug reactions;

[0116] ② The participants will be screened by the researchers, meet the inclusion and exclusion criteria, and sign the volunteer agreement to participate in the test. They must stop using similar functional cosmetics 48 hours before the start of the test, and must not engage in any other activities that may affect the selection and evaluation;

[0117] ③ Subjects who meet the inclusion criteria can be included in the study. Stop using similar functional cosmetics 48 hours before the test. On the test day, clean the test area with clean water and wipe the skin with a lint-free paper towel. Sit in a constant temperature and humidity environment (temperature 21±1℃, relative humidity 50±10%RH) for 30 minutes, then use a skin hemoglobin EI tester and Mexameter MX18 to collect the subject's skin basal value. Distribute samples and product instructions, and provide written test precautions;

[0118] ④The subjects filled out the consumer questionnaire, received the product and instructions for use, and then left the laboratory.

[0119] Day 7 (D7)

[0120] ① The subjects returned for a visit, cleansed their faces with facial cleansing products, and dried their skin with a lint-free paper towel. After sitting quietly in a constant temperature and humidity environment (temperature 21±1℃, relative humidity 50±10%RH) for 30 minutes, the D7 skin data values ​​of the subjects were collected;

[0121] ②The subjects filled in the self-assessment questionnaire on D7.

[0122] Day 14 14 )

[0123] ① The subject returned for a follow-up visit, cleansed his face with a facial cleanser, and dried his skin with a lint-free tissue. After sitting in a constant temperature and humidity environment (temperature 21±1°C, relative humidity 50±10%RH) for 30 minutes, D14 of the subject's skin was collected.

[0124] The comparative analysis results of the indicators at different times are shown in Table 10 below:

[0125] Table 10 Comparative analysis of various index data at different times (X±s, N=30)

[0126]

[0127] Table 11 Analysis of improvement rate and difference of each indicator in different periods

[0128]

[0129] (Note: *Significance marking method "ns" means no statistical difference, P>0.05; "*" means there is a significant difference, 0.01≤P<0.05; "**" means there is a significant difference, 0.001≤P<0.01)

[0130] from Figure 1 It can be seen that after applying the composition of Example 2 of the present invention for 14 days, the originally obvious red area on the face was significantly improved. As shown in Table 11, compared with before use, after 7 days of use, the red area value was significantly reduced (0.01≤P<0.05), a decrease of 5.21%, and after 14 days of use, the red area value was significantly reduced (0.001≤P<0.01), a decrease of 9.82%, indicating that the composition of Example 2 has a soothing effect.

[0131] Test Example 5: Irritation Test

[0132] Refer to SN / T 2329-2009 "Cosmetic Eye Irritation / Chicken Embryo Chorioallantoic Membrane Test" to conduct eye irritation test, i.e. safety test, on the product.

[0133] Experimental method: Fertilized chicken embryos less than 7 days old were used and incubated in an incubator at 37.6±0.5℃ and 50%-70% humidity for 9 days.

[0134] CAM preparation: Candle the egg, mark the location of the air chamber on the eggshell surface, peel off the marked eggshell with tweezers to expose the white egg membrane, and be careful not to damage the integrity of the egg membrane. Add 0.5mL of 0.9% NaCl solution to fully moisten the egg membrane, gently absorb the surface liquid with a paper towel, and carefully remove the inner membrane with tweezers to ensure that the vascular membrane is not damaged.

[0135] Reaction time method: Take 0.3 mL of the transparent test substance and evenly drip or smear it on the surface of CAM. After 3 minutes, use 0.9% NaCl solution to wash away the test substance, observe the degree of change of each toxic effect of CAM, and record the time of each toxic effect within 5 minutes. Set up 6 chicken embryos for each test substance, and set up 1 chicken embryo for each negative control (normal saline) and positive control (1% SDS).

[0136] The test samples of Example 4 and Comparative Example 7 were diluted to 1% solution for experimental testing. The irritation test scoring criteria are shown in Table 12.

[0137] Table 12: Rating criteria for irritation test

[0138]

[0139] Stimulus Rating Method:

[0140] The reaction time method was used to conduct the test, and the stimulation score (IS) was calculated using the following formula, with the result rounded to two decimal places:

[0141]

[0142] Where: secH (bleeding time)-----the average time when bleeding begins to occur on the CAM membrane, in seconds (s);

[0143] secL (vascular melting time) - the average time when vascular melting begins to be observed on the CAM membrane, in seconds (s);

[0144] secC (clotting time) - the average time for the onset of coagulation observed on the CAM membrane, in seconds (s).

[0145] Classify the eye irritation of the test substances according to the IS values ​​in accordance with Table 13.

[0146] Table 13: Evaluation of stimulation scoring results

[0147]

[0148]

[0149] The test results are shown in Table 14. The chicken embryo chorioallantoic membrane test of the moisturizing and soothing compositions of Examples 1 to 3 and Comparative Examples 1 to 6 showed no irritation, indicating that one, two or three combinations of DNA sodium, agarwood extract and juniper fruit extract are safe, non-toxic and low in irritation. The chicken embryo chorioallantoic membrane test results of Example 4 and Comparative Example 7 are shown in Table 14. Figure 2 and Figure 3 As shown, combined with Table 13, it can be seen that the IS value of Comparative Example 7 is 5.17, which is moderately irritating. The IS value of Example 4 after adding the moisturizing and soothing composition is 0.76. The moisturizing and soothing composition can significantly reduce the irritation of cleansing products.

[0150] Table 14: Statistics of irritation test results

[0151]

[0152] Test Example 6: Foam Test Experiment

[0153] The foam was tested and evaluated using the 2151 Roche foam tester. The specific test method is as follows:

[0154] (1) Turn on the thermostat. When the thermostat reaches a certain temperature, the temperature of the water bath in the tube jacket is stabilized at 40±0.5℃.

[0155] (2) Rinse the inner wall of the graduated tube with distilled water. Rinse thoroughly. Then rinse the tube wall with the test solution. Rinse thoroughly.

[0156] (3) Close the piston of the graduated tube and use another dropper to inject 50 mL of the test solution to the 50 mL mark. This test solution should be preheated to 40°C.

[0157] (4) Fill the dropper with 200 mL of test solution, which has been preheated to 40°C.

[0158] (5) Place the dropper on a prepared tube rack so that it is perpendicular to the cross section of the graduated tube, allowing the solution to flow to the center of the graduated tube. The outlet of the dropper should be placed on the 900 mm graduation line.

[0159] (6) Open the stopcock of the dropper to allow the solution to flow down. When the solution in the dropper has flowed out, start the stopwatch immediately and measure the foam height after 5, 10, and 15 minutes respectively. The foam value is expressed as the foam height.

[0160] (7) Repeat the above test 2 to 3 times. The wall of the device must be cleaned before each test to avoid affecting the data.

[0161] The test results are shown in Table 15.

[0162] Table 15: Statistics of foam height of different products

[0163]

[0164] It can be seen from the results in Table 15 that compared with Comparative Example 7, the product of Example 4 with the composition added has a higher foam height, stronger foaming ability, and richer foam, which indicates that the composition significantly improves the foaming ability of the cleansing product and makes the product foam richer.

[0165] The present invention first discovered a composition composed of DNA sodium, agarwood extract and juniper fruit extract, which has a significant synergistic effect on soothing and repairing, and has good soothing and repairing effects. The composition can not only be used to improve the irritation problem of cleansing products in cosmetics, but also can improve the foaming ability of cleansing products, making the product foam richer. The composition provided by the present invention has good application prospects in cosmetics.

[0166] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the given examples, those skilled in the art can modify or replace the technical solution of the present invention as needed without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A soothing and repairing composition, characterized in that: The components include the following by weight: 0.05-3.0 parts of sodium DNA, 1.0-15.0 parts of agarwood extract and 1.0-15.0 parts of juniper fruit extract; The agarwood extract is prepared by the following method, and the specific steps are as follows: The agarwood is crushed to a particle size of 40 to 80 meshes, the crushed agarwood is mixed with a volume fraction of 65 to 95% ethanol solution in a mass ratio of 1: (20 to 30), and then heated reflux extraction is performed, the reflux temperature is 55 to 65°C, and the extraction time is 110 to 130 minutes; after the extraction is completed and cooled, centrifugation is performed at a centrifugal speed of 5000 to 6000 rpm for 10 to 15 minutes to obtain a supernatant; the supernatant is concentrated under reduced pressure at a pressure of 0.04 to 0.1 MPa and a temperature of 40 to 60°C to an extract, and the obtained concentrate is dispersed and dissolved with butanediol, the mass ratio of the concentrate to butanediol is 1: (55 to 70), and after dissolution, it is filtered to obtain a filtrate that is an agarwood extract; The juniper berry extract is prepared by the following method, and the specific steps are as follows: The dried juniper berries are crushed and passed through a 40-100 mesh sieve. The crushed juniper berries are mixed with an ethanol solution with a volume fraction of 60-95% in a mass ratio of 1: (20-40), and extracted 2-4 times in a water bath at 65-75° C., each extraction lasting 60-120 minutes. After the extraction is completed, the mixture is centrifuged at a centrifugal speed of 5000-6000 rpm for 10-15 minutes, the supernatants are combined, and the mixture is concentrated under reduced pressure to an extract. 0.5-1.0 times the mass of butanediol is added, the mixture is stirred evenly, and the mixture is filtered through a filter membrane. The filtrate obtained is the juniper berry extract.

2. The composition according to claim 1, characterized in that The soothing and repairing composition comprises the following components by weight: 0.1 to 2.0 parts of sodium DNA, 5.0 to 10.0 parts of agarwood extract and 5.0 to 10.0 parts of juniper fruit extract.

3. The composition according to claim 2, characterized in that The soothing and repairing composition comprises the following components by weight: 1.0 part of sodium DNA, 8.0 parts of agarwood extract and 8.0 parts of juniper fruit extract.

4. The composition according to claim 1, characterized in that In the preparation method of agarwood extract, after dissolution, it is filtered through a 0.8 μm filter membrane, and the obtained filtrate is the agarwood extract.

5. The composition according to claim 1, characterized in that The preparation method of agarwood extract comprises the following specific steps: crushing the agarwood to a particle size of 40 to 80 meshes, mixing the crushed agarwood with an ethanol solution with a volume fraction of 80 to 95% in a mass ratio of 1:25, and then extracting by heating reflux, the reflux temperature is 55 to 65°C, and the extraction time is 120 min; after the extraction is completed and cooled, centrifuging at a centrifugal speed of 5500 rpm for 10 min to obtain a supernatant; concentrating the supernatant under reduced pressure at 0.08 MPa and 60°C to an extract, dispersing and dissolving the obtained concentrate with butanediol, the mass ratio of the concentrate to butanediol is 1:60, filtering with a 0.8μm filter membrane after dissolution, and the obtained filtrate is the agarwood extract.

6. The composition according to claim 1, characterized in that In the preparation method of the juniper berry extract, 0.8 μm filter membrane is used for filtration, and the obtained filtrate is the juniper berry extract.

7. The composition according to claim 1, characterized in that The preparation method of juniper fruit extract comprises the following specific steps: crushing dried juniper fruit, passing through a 100-mesh sieve, mixing the crushed juniper fruit with an ethanol solution with a volume fraction of 60-95% in a mass ratio of 1:30, extracting in a water bath at 65°C for 3 times, each time for 1 hour, centrifuging at 5500rpm for 15 minutes after the extraction, combining the supernatants, concentrating under reduced pressure to an extract, adding 0.6 times the mass of butanediol, stirring evenly, filtering with a 0.8μm filter membrane, and the obtained filtrate is the juniper fruit extract.

8. The method for preparing the soothing and repairing composition according to any one of claims 1 to 7, characterized in that: The steps include: According to weight parts, corresponding weight parts of sodium DNA, agarwood extract, and juniper fruit extract are mixed with water, and the amount of water is supplemented to 100 parts to obtain a soothing and repairing composition.

9. The preparation method according to claim 8, characterized in that: The water is deionized water.

10. Use of the soothing and repairing composition according to any one of claims 1 to 7 in the preparation of cosmetics.

11. The use according to claim 10, characterized in that The soothing and repairing composition is used in preparing cosmetics for soothing and repairing skin care.

12. A cosmetic, characterized in that: The cosmetic comprises the soothing and repairing composition according to any one of claims 1 to 7.

13. The cosmetic according to claim 12, characterized in that The cosmetics are soothing and repairing skin care cosmetics.

14. The cosmetic according to claim 13, characterized in that Based on the total mass of the soothing and repairing skin care cosmetic, the cosmetic contains 0.5 to 30% of the soothing and repairing composition.

15. The cosmetic according to claim 14, characterized in that Based on the total mass of the soothing and repairing skin care cosmetic, the cosmetic contains 2 to 20% of the soothing and repairing composition.

16. The cosmetic according to claim 13, characterized in that The types of soothing and repairing skin care cosmetics include cleansing milk / cream, essence water, facial mask, essence lotion, and essence cream.

17. The cosmetic according to claim 13, characterized in that The type of the soothing and repairing skin care cosmetic is essence.

18. The cosmetic according to claim 12, characterized in that The cosmetics include facial cleansers, lotions, creams, masks, shampoos and hair care products, and body care products.

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