Aerobic microorganism on-line culturing and adding equipment for combined system discharge port sewage

Abstract

The aerobic microorganism online culture and feeding equipment comprises a culture barrel, a microorganism module storage area is arranged on the outer side of the culture barrel, and a feeding port gate is arranged on the side wall of the top of the culture barrel; a microorganism module and a pushing device for pushing the microorganism module into the culture barrel from the feeding port gate are arranged in the microorganism module storage area. The target microorganisms needing to be cultured can be customized, different microorganism modules can be prepared, and the required dominant microorganism flora can be obtained by selecting the required microorganism modules and corresponding culture conditions. The filled microbial module is stable in property, is provided with a culture medium, can provide necessary nutrition for activating microbial strains and expanding culture propagation, and ensures rapid propagation of microorganisms. And the microbial module is easy to supplement, so that the culture period can be greatly shortened, and the starting of a terminal treatment system is accelerated.

Description

Technical Field

[0001] The invention belongs to the field of river and lake water environment management and restoration, and relates to microbial cultivation in combined sewage treatment, and in particular to aerobic microbial online cultivation and dosing equipment for combined outfall sewage. Background Art

[0002] Since the old residential areas in general cities are all under the combined sewer system, there are a large number of combined sewer outlets around the inner lakes of the city. On rainy days, the combined sewage enters the city lake, causing serious damage to the water in the lake, which is an important factor causing the black and smelly urban water. In order to ensure that the water quality of the lake meets the standards, in addition to the rainwater and sewage diversion transformation at the source, an enhanced treatment unit will be set up at the end of the combined sewer outlet. Commonly used enhanced treatment units are generally biological treatment processes, which are relatively friendly to the environment and will not cause secondary pollution. However, since the effluent from the combined sewer outlet is closely related to the intensity and frequency of rainfall, the outlet water quality and water volume are extremely unstable. The traditional biological treatment process has a long microbial growth cycle and a slow cultivation speed. When it cannot be operated continuously, the entire system starts slowly, and the treatment effect of the combined sewer overflow sewage with a large water volume and water quality impact load is poor. In addition, the dominant microbial flora that grows automatically through in-situ sewage is not necessarily the optimal microbial flora required. Therefore, in the terminal biological treatment area, it is necessary to add a set of online microbial cultivation and dosing equipment to cultivate the target dominant bacterial flora, prepare in advance to deal with the impact of outlet water quality, and improve the stability of the terminal enhancement system. Summary of the invention

[0003] The purpose of the present invention is to provide an aerobic microbial online cultivation and dosing device for combined sewer outlet sewage in view of the above-mentioned problems in the prior art. The present invention forms a high-density microbial flora solution by replacing different microbial modules and adjusting the corresponding temperature, dissolved oxygen and pH conditions for expansion cultivation. The high-density microbial solution is put into the combined sewer terminal reaction zone through the outlet pipe to increase the overall microbial count, accelerate the start-up of the combined sewer terminal enhanced treatment system, and stabilize the pollutant removal effect.

[0004] The object of the present invention is to provide an aerobic microorganism online cultivation and dosing device for combined outfall sewage in view of the above-mentioned problems existing in the prior art.

[0005] The above-mentioned purpose of the present invention is achieved by the following technical means:

[0006] The invention discloses an aerobic microorganism online culture and dosing device for combined outfall sewage, comprising a culture barrel, a microorganism module storage area is arranged outside the culture barrel, a feed port gate is opened on the top side wall of the culture barrel, a microorganism module is arranged in the microorganism module storage area, and a pushing device for pushing the microorganism module from the feed port gate into the culture barrel is arranged.

[0007] As mentioned above, the pushing device includes a lifting unit, a vertical telescopic portion of the lifting unit is provided with a support plate, microorganism modules are stacked on the support plate, and a pushing unit is provided on the inner wall of the microorganism module storage area opposite to the feed port gate.

[0008] As described above, a porous plate is provided in the culture barrel below the feed port gate, and a spray pipe is provided on the top of the porous plate. The spray pipe is connected to the water outlet of the water pump through the water inlet pipe, and the water inlet of the water pump is connected to the water pump, and a filter head is provided on the water pump.

[0009] As mentioned above, multiple strings of spherical fillers are suspended below the perforated plate.

[0010] As described above, a perforated aeration pipe is arranged below the spherical filler in the culture barrel, an adjustment area is arranged above the culture barrel, an air compressor is arranged in the adjustment area, and the perforated aeration pipe is connected to the air compressor.

[0011] As described above, a heating plate is arranged at the bottom of the culture barrel, an acid dosing system and an alkali dosing system are arranged in the adjustment area, the outlet pipes of the acid dosing system and the alkali dosing system are connected to the culture barrel or the spray pipe, and a pH probe and a temperature probe are arranged in the culture barrel.

[0012] As mentioned above, the microbial module is square.

[0013] As mentioned above, the microorganism module comprises an outer package and powdered microorganisms and culture medium wrapped by the outer package.

[0014] As mentioned above, the temperature in the culture barrel ranges from 35 to 38° C., the dissolved oxygen content ranges from 1 mg / L to 2 mg / L, and the pH ranges from 7.0 to 7.5.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The target microorganisms to be cultivated can be customized, different microbial modules can be prepared, and the required microbial modules and corresponding cultivation conditions can be selected to obtain the required dominant microbial flora. The loaded microbial modules are stable in nature and come with their own culture medium, which can provide the necessary nutrients for activating microbial strains and expanding cultivation and reproduction, ensuring rapid reproduction of microorganisms. In addition, the microbial modules are easy to replenish, which can greatly reduce the cultivation cycle and speed up the startup of the terminal treatment system. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of the present invention.

[0018] Among them, 1-microorganism module storage area; 11-tray; 12-lifting unit; 13-pushing unit; 14-feeding port; 15-microorganism module; 21-filter head; 22-water pump; 23-water inlet pipe; 24-feeding port gate; 25-spray pipe; 26-porous plate; 3-culture barrel; 31-spherical filler; 32-perforated aeration pipe; 4-regulation area; 41-air compressor; 42-acid dosing system; 43-alkali dosing system; 44-controller; 45-pH probe; 46-temperature probe; 47-heating plate; 51-water outlet valve; 52-water outlet pipe. DETAILED DESCRIPTION

[0019] In order to facilitate those skilled in the art to understand and implement the present invention, the present invention is further described in detail below in conjunction with embodiments. It should be understood that the implementation examples described herein are only used to illustrate and explain the present invention and are not used to limit the present invention.

[0020] Embodiment 1:

[0021] The aerobic microorganism online culture and dosing equipment for combined outfall sewage comprises a culture barrel 3, the culture barrel 3 is used to culture microorganisms, a microorganism module storage area 1 is arranged outside the culture barrel 3, the microorganism module storage area 1 is used to store microorganism modules 15, a feed port gate 24 is provided on the top side wall of the culture barrel 3, the microorganism module storage area 1 is provided with microorganism modules 15 and a pushing device for pushing the microorganism module 15 from the feed port gate 24 into the culture barrel 3. A replenishment port is arranged at the top of the microorganism module storage area 1, and the microorganism module 15 is replenished through the replenishment port. The feed port gate 24 can be a one-way valve, which can only enter the culture barrel 3 from the microorganism module storage area 1, and can prevent water from flowing back into the microorganism module storage area 1.

[0022] In some embodiments, the pushing device includes a lifting unit 12, a support plate 11 is provided on the vertical telescopic portion of the lifting unit 12, a microorganism module 15 is stacked on the support plate 11, and a pushing unit 13 is provided on the inner wall of the microorganism module storage area 1 opposite to the feed port gate 24. The microorganism modules 15 are stacked in blocks on the support plate 11, and when the microorganism modules 15 need to be put in, the lifting unit 12 pushes the microorganism modules 15 up to the feed port gate 24, and the pushing unit 13 pushes the uppermost microorganism modules 15 from the feed port gate 24 into the culture barrel 3.

[0023] In some embodiments, a porous plate 26 is provided in the culture barrel 3 below the feed port gate 24, and a spray pipe 25 is provided on the top of the porous plate 26 of the culture barrel 3. The spray pipe 25 is connected to the water outlet of the water pump 22 through the water inlet pipe 23, and the water inlet of the water pump 22 is connected to the water pumping pipe, and a filter head 21 is provided on the water pumping pipe. When the microorganism module 15 is pushed from the feed port gate 24 into the culture barrel 3 and falls on the porous plate 26, the water pump 22 is started to pump water, and the water is sprayed on the microorganism module 15 through the spray pipe 25. After the microorganism module 15 is dissolved, it falls into the culture barrel 3 for culture, and the filter head 21 prevents foreign matter from entering.

[0024] In some embodiments, multiple strings of spherical fillers 31 are suspended below the porous plate 26. The spherical fillers 31 provide an attachment bed for the growth of microorganisms.

[0025] In some embodiments, a perforated aeration pipe 32 is provided in the culture barrel 3 below the spherical filler 31, an adjustment area 4 is provided above the culture barrel 3, an air compressor 41 is provided in the adjustment area 4, and the perforated aeration pipe 32 is connected to the air compressor 41. The compressed gas output by the air compressor 41 is aerated through the perforated aeration pipe 32.

[0026] In some embodiments, a heating plate 47 is provided at the bottom of the culture barrel 3, an acid dosing system 42 and an alkali dosing system 43 are provided in the regulating area 4, the outlet pipes of the acid dosing system 42 and the alkali dosing system 43 are both connected to the culture barrel 3, a pH probe 45 and a temperature probe 46 are provided in the culture barrel 3, a controller is connected to the pH probe 45 and the temperature probe 46, the controller is also connected to the outlet pumps on the outlet pipes of the acid dosing system 42 and the alkali dosing system 43, the controller is also connected to the heating plate 47, the controller is also connected to the lifting unit 12, the pushing unit 13, the air compressor 41 and the water pump 22. The pH value and temperature of the culture solution in the culture barrel 3 are read by the pH probe 45 and the temperature probe 46, the pH value of the culture solution is adjusted to the set pH value range by controlling the switches of the outlet pumps on the outlet pipes of the acid dosing system 42 and the alkali dosing system 43, and the temperature of the culture solution is adjusted to the set temperature range by controlling the current to be passed on and off the heating plate 47. The controller is also used to control the actions of the lifting unit 12 and the pushing unit 13, and to control the start and stop of the water pump 22. The lifting unit 12 and the pushing unit 13 are both electric telescopic devices, and both include a fixed part and a telescopic part.

[0027] The bottom of the culture bucket 3 is connected to a water outlet pipe 52 , and a water outlet valve 51 is provided on the water outlet pipe 52 .

[0028] When the present invention is used, the required microorganism module 15 is placed in the microorganism module storage area 1, and microorganism cultivation is started about one week before the rainy season. The microorganism module 15 rises to the feed port gate 24 through the lifting unit, and is sent into the culture barrel 3 through the pushing unit 13. At the same time, the water pump 22 is started, and water is taken from the lake to the equipment spray pipe 25. The outer packaging of the microorganism module 15 begins to dissolve when it meets water, and the microorganism module 15 dissolves in the water and enters the culture barrel 3 together. The operation of the water pump 22 is controlled to ensure the water level of the culture barrel 3. The controller collects data in the culture barrel 3 according to the temperature probe 46 and the pH probe 45, and operates the heating plate 47, the acid dosing system 42 and the alkali dosing system 43 according to the data to ensure the corresponding indicators in the culture barrel 3. The air compressor 41 is started with the water inlet to ensure the dissolved oxygen content in the culture area. The aeration volume and wind pressure are set in advance according to the type of microorganism to ensure that the dissolved oxygen content in the culture barrel 3 is always in the required aerobic state. After 5-7 days of cultivation, the high-density microbial solution can be added to the required area through the outlet pipe 52 to proceed to the next step.

[0029] The microbial module 15 includes an outer package, and powdered microorganisms and culture media wrapped in the outer package. The microbial module 15 is square in shape as a whole, and the outer package is encapsulated with a material that can be dissolved in water, such as water-soluble paper, polyvinyl alcohol film, starch-based film, etc. The powdered microorganism is a dry powder made of Bacillus cereus (the deposit number is: CCTCC NO: M2021207, the name of the depository is China Center for Type Culture Collection, the address is Wuhan, China, Wuhan University, the deposit date is March 4, 2021, and the classification name is Bacillus cereus WF01); the culture medium includes a carbon source (glucose in this embodiment), a nitrogen source (peptone in this embodiment), an inorganic salt (a mixture of sodium chloride and yeast extract in this embodiment), a growth factor (B vitamins in this embodiment) and a coagulant (agar in this embodiment) and is made after high temperature and high pressure sterilization.

[0030] In this embodiment, the weight ratio of powdered microorganisms to culture medium is (1:10) to (1:20); the weight ratio of carbon source, nitrogen source, inorganic salt, growth factor and coagulant is 20:10:5:1:1. A single microorganism module 15 weighs 200 g.

[0031] In some embodiments, the culture barrel 3 is a square barrel structure, and the temperature in the culture barrel 3 is controlled between 35 and 38° C. by controlling the heating plate 47. The air compressor 41 is controlled by the controller to control the dissolved oxygen content in the culture barrel 3 to be between 1 mg / L and 2 mg / L. The acid solution dosing system 42 and the alkali solution dosing system 43 are controlled by the controller to control the pH value in the culture barrel 3 to be between 7.0 and 7.5.

[0032] As another embodiment, the liquid outlet pipes of the acid solution adding system 42 and the alkali solution adding system 43 are both connected to the spray pipe 25 to facilitate easy and uniform entry into the culture barrel 3.

[0033] The advantages and positive effects of the present invention are: the present invention can effectively cultivate a high-density microbial liquid in the preparation stage. After the liquid is added to the lake, it can quickly become a dominant strain, achieving the design purpose. The microbial module used in the present invention can be easily supplemented, and the use of the microbial module can ensure the accurate expansion of the target microorganism without being affected by other bacteria. After the controller sets the parameters, no additional operation is required, and the overall control is simple.

[0034] Embodiment 2:

[0035] There are combined sewer outlets distributed along the roadsides of a certain inner lake in a certain city. In order to solve the problem of excessive overflow of sewage into the lake, an enhanced ecological floating island purification area is set at the end of the outlet. Enhanced ecological floating islands, aeration devices and the equipment of the present invention are set up in the purification area. A control area is set up, and there is no equipment of the present invention in the control area, which only relies on the in-situ growth of the water body. Three days after the overflow pollution caused by the first rain entered the lake, the biofilm of the suspended filler under the enhanced ecological floating island was observed with the naked eye. It can be clearly found that in the area where the equipment of the present invention is added, the microbial community on the filler under the ecological floating island is richer. At the same time, the water quality of the water body in the area is tested, and it can be found that the water quality in the area where the equipment of the present invention is added has basically returned to the previous level. After 3-5 days of microbial culture using the equipment of the present invention, the concentration of microorganisms in the culture barrel can reach 5×10^9cfu / mL.

[0036] Table 1 is a comparison table of in situ water quality results of different groups after 3 days

[0037]

[0038] It should be noted that the embodiments described in the present invention are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the described embodiments or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the attached claims.

Claims

1. An aerobic microorganism online culture and dosing device for combined outfall sewage, comprising a culture tank (3), characterized in that: A microorganism module storage area (1) is arranged outside the culture barrel (3), a feed port gate (24) is provided on the top side wall of the culture barrel (3), and a microorganism module (15) and a pushing device for pushing the microorganism module (15) from the feed port gate (24) into the culture barrel (3) are arranged inside the microorganism module storage area (1).

2. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 1, characterized in that: The pushing device comprises a lifting unit (12), a support plate (11) is arranged on the vertical telescopic part of the lifting unit (12), a microorganism module (15) is stacked on the support plate (11), and a pushing unit (13) is arranged on the inner wall of the microorganism module storage area (1) opposite to the feed port gate (24).

3. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 1, characterized in that: A porous plate (26) is arranged in the culture barrel (3) below the feed port gate (24); a spray pipe (25) is arranged on the top of the culture barrel (3) on the porous plate (26); the spray pipe (25) is connected to the water outlet of the water pump (22) through a water inlet pipe (23); the water inlet of the water pump (22) is connected to a water pump pipe, and a filter head (21) is arranged on the water pump pipe.

4. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 3, characterized in that: A plurality of strings of spherical fillers (31) are suspended below the porous plate (26).

5. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 4, characterized in that: A perforated aeration pipe (32) is arranged below the spherical filler (31) in the culture barrel (3), an adjustment area (4) is arranged above the culture barrel (3), an air compressor (41) is arranged in the adjustment area (4), and the perforated aeration pipe (32) is connected to the air compressor (41).

6. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 5, characterized in that: A heating plate (47) is arranged at the bottom of the culture barrel (3); an acid solution adding system (42) and an alkali solution adding system (43) are arranged in the regulating area (4); the liquid outlet pipes of the acid solution adding system (42) and the alkali solution adding system (43) are both connected to the culture barrel (3) or the spray pipe (25); and a pH probe (45) and a temperature probe (46) are arranged in the culture barrel (3).

7. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 1, characterized in that: The microorganism module (15) is square.

8. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 1, characterized in that: The microorganism module (15) comprises an outer package and powdered microorganisms and culture medium wrapped in the outer package.

9. The aerobic microorganism online cultivation and dosing equipment for combined outfall sewage according to claim 1, characterized in that: The temperature in the culture barrel (3) is in the range of 35 to 38° C., the dissolved oxygen content is in the range of 1 mg / L to 2 mg / L, and the pH value is in the range of 7.0 to 7.5.

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