Device and method for medical Raman spectrum detection

Abstract

The invention provides a device and a method for medical Raman spectrum detection, and belongs to the technical field of Raman spectrum detection. The device comprises a laser used for emitting laser to a sample; the Raman probe device is configured to comprise a laser excitation light path and a Raman signal collection light path, the laser excitation light path is used for transmitting the laser emitted by the laser to the sample, and the Raman signal collection light path is used for collecting Raman signal light generated after the laser excites the sample; and the spectrograph is used for receiving the Raman signal light from the Raman signal collection light path and carrying out spectrogram collection and analysis on the Raman signal light. According to the device for medical Raman spectrum detection, the position consistency of Raman signal light of an excitation sample and a collection sample can be ensured, signal crosstalk is effectively blocked, the purity and the singleness of Raman signal collection are realized, and the accuracy and the reliability of data analysis are improved.

Description

Technical Field

[0001] The present disclosure relates to the technical field of Raman spectroscopy detection, and in particular to a device and method for medical Raman spectroscopy detection. Background Art

[0002] Raman spectroscopy is an analytical technique based on the Raman scattering effect. It analyzes scattered spectra with different frequencies from the incident light and uses the obtained molecular vibration and rotation information to study the molecular composition structure. It has been well developed and applied in many scientific fields. Raman probes are an important medium for the actual application of Raman spectroscopy in clinical practice. They can quickly and non-invasively detect tissue samples and play an important role in disease diagnosis, treatment and subsequent monitoring. However, in order to measure the Raman spectra of substances in the body more quickly, in real time, lightly and pollution-free, and accurately in medical testing, it is necessary to achieve the characteristics of lightweight, easy replacement and easy handling of the detection device.

[0003] In the prior art, Raman probes use a dual-fiber excitation and collection method or a multi-fiber N+1 collection and excitation method to detect samples or tissues in vitro. The advantage of this structure is that the collection fiber and the excitation fiber are independent, which reduces signal crosstalk. However, since the dichroic mirrors and long-wave filter used in most Raman probes have poor filtering effects on laser emission light and Rayleigh scattered light, it is very easy to have a high background noise and large fluctuations in the spectrum at the short wavelength after the Raman signal is collected, making it difficult to analyze. When used for in vivo detection, the optical fiber needs to be wrapped in the endoscope structure pipeline for detection, which makes the operation process time-consuming and labor-intensive, and the cost is high. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a device and method for medical Raman spectroscopy detection, so as to facilitate in vivo detection of human or living bodies, achieve the purity and singleness of Raman signal acquisition, and improve the accuracy and reliability of data analysis.

[0005] In order to achieve the above-mentioned invention object, the present invention adopts the following technical solutions:

[0006] A device for medical Raman spectroscopy detection, comprising:

[0007] A laser, used to emit laser light to the sample;

[0008] A Raman probe device is configured to include a laser excitation optical path and a Raman signal collection optical path, wherein the laser excitation optical path is used to transmit the laser light emitted by the laser to the sample, and the Raman signal collection optical path is used to collect the Raman signal light generated after the laser excites the sample;

[0009] The spectrometer is used to receive the Raman signal light from the Raman signal collection optical path and perform spectrum collection and analysis on the Raman signal light.

[0010] In an exemplary embodiment of the present disclosure, the Raman probe device comprises:

[0011] A laser excitation optical fiber, used for receiving the laser light emitted by the laser;

[0012] A single optical fiber, used to transmit the laser to the sample and collect the Raman signal light generated after the laser excites the sample;

[0013] A light splitting and beam combining lens group is provided between the laser excitation optical fiber and the single optical fiber, and the light splitting and beam combining lens group is used to shape and filter the laser output by the laser excitation optical fiber, and to shape and filter the Raman signal light output by the single optical fiber;

[0014] The Raman signal collecting optical fiber is used to filter the Raman signal light output by the light splitting and beam combining lens group, and transmit the filtered Raman signal light to the spectrometer.

[0015] In an exemplary embodiment of the present disclosure, the light splitting and beam combining lens group comprises:

[0016] A first lens, a first filter and a reflector are sequentially arranged along the direction in which the laser excitation optical fiber outputs the laser, wherein the first lens and the first filter are perpendicular to the optical path, and the reflector is arranged at an angle of 45 degrees to the opposite direction of the laser input direction;

[0017] a dichroic mirror located on the reflected light path of the reflector, the dichroic mirror and the reflector are parallel to each other, a third lens is vertically arranged on the reflected light path of the dichroic mirror, a second filter and a second lens are vertically arranged in sequence along the reverse direction of the reflected light path, the single optical fiber is located on a side of the third lens away from the dichroic mirror, and the Raman signal collection optical fiber is located on a side of the second lens away from the dichroic mirror;

[0018] The reflection light path of the dichroic mirror is parallel to the output direction of the Raman signal light.

[0019] In an exemplary embodiment of the present disclosure, the laser excitation optical path includes the first lens, the first filter, the reflector, the dichroic mirror, the third lens and the single optical fiber in sequence;

[0020] The Raman signal collection optical path includes the single optical fiber, the third lens, the dichroic mirror, the second filter and the second lens in sequence;

[0021] The single optical fiber, the third lens, and the dichroic mirror are common components of the laser excitation optical path and the Raman signal collection optical path.

[0022] In an exemplary embodiment of the present disclosure, both the first lens and the third lens are aspherical lenses;

[0023] The surface of the reflector is plated with a high reflection film.

[0024] In an exemplary embodiment of the present disclosure, a diameter of the second lens is greater than diameters of the first lens and the third lens.

[0025] In an exemplary embodiment of the present disclosure, the dichroic mirror is used to transmit a first wavelength and to reflect a second wavelength;

[0026] The first wavelength is a long wave, and the second wavelength is a short wave.

[0027] In an exemplary embodiment of the present disclosure, a coupling member is provided at one end of the single optical fiber close to the third lens, and the coupling member can drive the single optical fiber to move back and forth along the output direction of the Raman signal light.

[0028] In an exemplary embodiment of the present disclosure, the Raman signal collection optical fiber is configured as an optical fiber etched with a fiber Bragg grating.

[0029] A method for medical Raman spectroscopy detection, using any of the above-mentioned devices for medical Raman spectroscopy detection to perform Raman spectroscopy detection, comprising:

[0030] Step S1: using a laser to emit laser;

[0031] Step S2: using a laser excitation optical fiber to transmit the laser to the light splitting and beam combining lens group;

[0032] Step S3: shaping the laser into a collimated beam using a first lens, filtering the beam using a first filter, then changing the beam path by reflection through a reflector and a dichroic mirror, focusing the beam into a single optical fiber through a third lens, and transmitting the laser to the sample using the single optical fiber;

[0033] Step S4: the sample generates Raman signal light after being excited by the laser, and the single optical fiber transmits the Raman signal light to the light splitting and beam combining lens group;

[0034] Step S5: using a third lens to shape the Raman signal light into a collimated light beam, the Raman signal light is sequentially filtered by a dichroic mirror for primary filtering, filtered by a second filter, and then focused and coupled to a Raman signal collection optical fiber through a second lens for tertiary filtering;

[0035] Step S6: using a spectrometer to collect and analyze the spectrum of the Raman signal light after the three-time filtering.

[0036] Beneficial effects of the present disclosure:

[0037] (1) The present invention sets a single optical fiber at the output end of the Raman probe device, and uses the single optical fiber to excite the sample and collect the Raman signal, thereby effectively saving costs and achieving lightweight and simplification of the output end of the Raman probe. The use of a single optical fiber can ensure the position consistency of the Raman signal light of the sample and the sample, thereby improving the accuracy of the overall analysis.

[0038] (2) The present invention enables users to quickly and conveniently operate and replace optical fibers, and is more suitable for in vivo detection of human or animal bodies.

[0039] (3) The present invention provides an optical fiber with a fiber Bragg grating at the collecting end of the Raman probe device to perform a third filtering of the reflected light, Rayleigh scattering and other stray light returned by the excitation light, thereby effectively blocking the crosstalk of the signal, reducing the Raman signal generated by the single optical fiber itself, achieving the purity and singleness of the Raman signal collection, improving the signal-to-noise ratio, and increasing the accuracy and reliability of data analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] The accompanying drawings herein are incorporated into the specification and constitute a part of the specification, illustrate embodiments consistent with the present disclosure, and together with the specification are used to explain the principles of the present disclosure. Obviously, the accompanying drawings described below are only some embodiments of the present disclosure, and for ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without creative work.

[0041] Figure 1 This is a schematic diagram of the structure of a device for medical Raman spectroscopy detection in one embodiment of the present disclosure;

[0042] Figure 2 The present invention is a schematic flow chart of a method for medical Raman spectroscopy detection in one embodiment of the present invention.

[0043] Description of reference numerals:

[0044] 1. Laser; 2. Sample; 3. Raman probe device; 4. Spectrometer; 5. Laser excitation optical fiber; 6. Single optical fiber; 7. Light splitting and beam combining lens group; 8. Raman signal collection optical fiber; 9. First lens; 10. First filter; 11. Reflector; 12. Third lens; 13. Dichroic mirror; 14. Second filter; 15. Second lens; 16. Coupling piece. DETAILED DESCRIPTION

[0045] Example embodiments will now be described more fully with reference to the accompanying drawings. However, the example embodiments can be implemented in a variety of forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that the present disclosure will be comprehensive and complete and fully convey the concepts of the example embodiments to those skilled in the art. The same reference numerals in the figures represent the same or similar structures, and thus their detailed description will be omitted. In addition, the drawings are only schematic illustrations of the present disclosure and are not necessarily drawn to scale.

[0046] Although relative terms such as "upper" and "lower" are used in this specification to describe the relative relationship of one component of the illustration to another component, these terms are used in this specification only for convenience, such as according to the orientation of the examples described in the drawings. It is understood that if the device of the illustration is turned upside down, the component described as "upper" will become the component "lower". When a structure is "on" other structures, it may mean that the structure is formed integrally on the other structure, or that the structure is "directly" disposed on the other structure, or that the structure is "indirectly" disposed on the other structure through another structure.

[0047] The terms "a", "an", "the", "said" and "at least one" are used to indicate the presence of one or more elements / components / etc.; the terms "including" and "having" are used to express an open-ended inclusive meaning and mean that additional elements / components / etc. may exist in addition to the listed elements / components / etc.; the terms "first", "second" and "third" etc. are used merely as labels and are not intended to limit the quantity of their objects.

[0048] The present disclosure provides a device for medical Raman spectroscopy detection, see Figure 1 The device for medical Raman spectroscopy detection includes: a laser 1, which is used to emit laser light to a sample 2; a Raman probe device 3, which is configured to include a laser excitation optical path and a Raman signal collection optical path, the laser excitation optical path is used to transmit the laser light emitted by the laser 1 to the sample 2, and the Raman signal collection optical path is used to collect the Raman signal light generated after the laser excites the sample 2; a spectrometer 4, which is used to receive the Raman signal light from the Raman signal collection optical path and perform spectrum collection and analysis on the Raman signal light.

[0049] In the embodiment of the present disclosure, the device for medical Raman spectroscopy detection is composed of a laser 1, a sample 2, a Raman probe device 3 and a spectrometer 4. The laser 1 and the spectrometer 4 are located at the input end of the Raman probe device 3, and the sample 2 is located at the output end of the Raman probe device 3. The Raman probe device 3 includes a laser excitation optical path and a Raman signal collection optical path, and the laser excitation optical path and the Raman signal collection optical path are parallel to each other and partially overlap. The laser 1 emits a laser to the sample 2. After the Raman probe device 3 receives the laser, the laser is transmitted to the sample 2 through the laser excitation optical path. After the sample 2 is excited by the laser, Raman signal light is generated. The generated Raman signal light is reversely transmitted to the spectrometer 4 through the Raman signal collection optical path. The spectrometer 4 collects and analyzes the received Raman signal light to complete the Raman spectroscopy detection.

[0050] Compared with existing Raman probes, the device for medical Raman spectroscopy detection achieves lightweight and simplification of the output end of the Raman probe, and the use of a single optical fiber can ensure the position consistency of the Raman signal light of the exciting sample and the collected sample, thereby improving the accuracy of the overall analysis; it can effectively block the crosstalk of the signal, reduce the Raman signal generated by the single optical fiber itself, achieve the purity and singleness of the Raman signal collection, improve the signal-to-noise ratio, and increase the accuracy and reliability of data analysis; it can allow users to quickly and conveniently operate and replace optical fibers, and is more suitable for the detection of living humans or animals.

[0051] In one embodiment of the present disclosure, see Figure 1 , the Raman probe device 3 includes: a laser excitation optical fiber 5, which is used to receive the laser emitted by the laser 1; a single optical fiber 6, which is used to transmit the laser to the sample 2 and collect the Raman signal light generated after the laser excites the sample 2; a light splitting and beam combining lens group 7, which is arranged between the laser excitation optical fiber 5 and the single optical fiber 6, and the light splitting and beam combining lens group 7 is used to shape and filter the laser output from the laser excitation optical fiber 5, and shape and filter the Raman signal light output from the single optical fiber 6; a Raman signal collecting optical fiber 8, which is used to filter the Raman signal light output from the light splitting and beam combining lens group 7, and transmit the filtered Raman signal light to the spectrometer 4. In this way, by arranging the single optical fiber 6 at the output end of the Raman probe device 3, the sample 2 is excited and the Raman signal light is collected through the single optical fiber 6, so as to ensure the position consistency of the Raman signal light of the sample 2 and the sample 2; by arranging the Raman signal collecting optical fiber 8 at the collection end of the Raman probe device 3, the crosstalk of the signal is effectively blocked, and the purity and singleness of the Raman signal collection are achieved.

[0052] Optionally, the laser excitation optical fiber 5 and the Raman signal collection optical fiber 8 are located on one side of the light splitting and beam combining lens group 7 , and the single optical fiber 6 is located on the other side of the light splitting and beam combining lens group 7 .

[0053] Optionally, the laser excitation optical fiber 5 and the Raman signal collection optical fiber 8 are parallel to each other.

[0054] Optionally, the Raman signal collection optical fiber 8 is arranged coaxially with the single optical fiber 6 .

[0055] In one embodiment of the present disclosure, see Figure 1 The light splitting and beam combining lens group 6 includes: a first lens 9, a first filter 10 and a reflector 11 which are sequentially arranged along the direction in which the laser excitation optical fiber 5 outputs the laser, the first lens 9 and the first filter 10 are perpendicular to the optical path, and the reflector 11 is arranged at an angle of 45 degrees to the direction opposite to the laser input direction; a dichroic mirror 13 is located on the reflected light path of the reflector 11, the dichroic mirror 13 and the reflector 11 are parallel to each other, a third lens 12 is vertically arranged on the reflected light path of the dichroic mirror 13, a second filter 14 and a second lens 15 are sequentially vertically arranged in the opposite direction of the reflected light path, the single optical fiber 6 is located on the side of the third lens 12 away from the dichroic mirror 13, and the Raman signal collecting optical fiber 8 is located on the side of the second lens 15 away from the dichroic mirror 13; the reflected light path of the dichroic mirror 13 is parallel to the output direction of the Raman signal light. In this way, the laser light entering the Raman probe device 3 can be shaped and filtered, and the transmission path of the laser beam can be changed to facilitate laser excitation of the sample 2; at the same time, the Raman signal light entering the Raman probe device 3 can be shaped and filtered to achieve the purity and singleness of Raman signal acquisition.

[0056] Optionally, the first filter 10 is a narrowband filter, and the second filter 14 is a long-pass filter.

[0057] It can be understood that the laser 1, the laser excitation fiber 5, the first lens 9, the first filter 10 and the reflector 11 are distributed in a straight line in sequence; the spectrometer 4, the Raman signal collection fiber 8, the second lens 15, the second filter 14, the dichroic mirror 13, the third lens 12, the single optical fiber 6 and the sample 2 are distributed in a straight line in sequence.

[0058] In one embodiment of the present disclosure, see Figure 1 , the laser excitation optical path includes the first lens 9, the first filter 10, the reflector 11, the dichroic mirror 13, the third lens 12 and the single optical fiber 6 in sequence; the Raman signal collection optical path includes the single optical fiber 6, the third lens 12, the dichroic mirror 13, the second filter 14 and the second lens 15 in sequence; the single optical fiber 6, the third lens 12 and the dichroic mirror 13 are common components of the laser excitation optical path and the Raman signal collection optical path. In this way, the Raman probe device 3 can be simplified and lightweight, which is convenient for users to perform detection operations and can be more flexibly applied to human or living body detection.

[0059] In one embodiment of the present disclosure, see Figure 1The first lens 9 and the third lens 12 are both aspherical lenses. In this way, the laser and Raman signal can be optically shaped into a collimated beam to avoid optical path distortion.

[0060] Optionally, a high-reflection film is coated on the surface of the reflector 11. In this way, the reflectivity of the reflector 11 to the laser can be improved.

[0061] In one embodiment of the present disclosure, see Figure 1 The diameter of the second lens 15 is larger than the diameters of the first lens 9 and the third lens 12. In this way, the filtered Raman signal light can be focused and coupled into the Raman signal collection optical fiber 8 as much as possible, further avoiding optical path distortion.

[0062] In one embodiment of the present disclosure, the dichroic mirror 13 is used to transmit the first wavelength and to reflect the second wavelength; the first wavelength is a long wavelength and the second wavelength is a short wavelength. In this way, the Raman signal light can be initially filtered to filter out part of the laser reflected light and Rayleigh scattered light in the Raman signal light, and the filtering rate can reach 40% to 60%, thereby improving the purity and singleness of the Raman signal light collection.

[0063] It can be understood that the second filter 14 performs secondary filtering on the Raman signal light after the initial filtering, thereby achieving secondary filtering of part of the laser reflected light and Rayleigh scattered light in the Raman signal light. The second filter 14 can filter out 90% to 95% again on the basis of the filtering rate of the dichroic mirror 13, thereby improving the purity and singleness of the Raman signal light collection.

[0064] In one embodiment of the present disclosure, see Figure 1 A coupling member 16 is provided at one end of the single optical fiber 6 close to the third lens 12, and the coupling member 16 can drive the single optical fiber 6 to move back and forth along the Raman signal light output direction. In this way, the position of the single optical fiber 6 can be adjusted to improve the efficiency of laser coupling into the single optical fiber 6.

[0065] Optionally, the single optical fiber 6 and the coupling member 16 are detachably mounted.

[0066] It is understandable that, depending on the sample 2 , a single optical fiber 6 with different lengths or core diameters may be selected.

[0067] In one embodiment of the present disclosure, see Figure 1 The Raman signal collection optical fiber 8 is configured as an optical fiber etched with a fiber Bragg grating. In this way, the Raman signal light after the secondary filtering can be filtered three times, and part of the laser reflected light and Rayleigh scattered light in the Raman signal light can be filtered three times. The Raman signal collection optical fiber 8 can filter out 99% again on the basis of the filtering rate of the second filter 14, thereby improving the purity and singleness of the Raman signal light collection.

[0068] It can be understood that the Raman signal collection optical fiber 8 can filter out part of the Raman light generated by the single optical fiber 6 itself, further improving the purity and singleness of the Raman signal light collection, and improving the accuracy and reliability of data analysis.

[0069] It is understandable that, depending on the sample 2, a Raman signal collection optical fiber 8 with a different central wavelength, bandwidth or reflectivity can be selected.

[0070] The present disclosure provides a method for medical Raman spectroscopy detection, wherein the device for medical Raman spectroscopy detection described in any of the above embodiments is used to perform Raman spectroscopy detection, see Figure 2 ,include:

[0071] Step S1: using laser 1 to emit laser;

[0072] Step S2: using the laser excitation optical fiber 5 to transmit the laser to the light splitting and beam combining lens group 7;

[0073] Step S3: The laser is shaped into a collimated beam by a first lens 9, and the beam is filtered by a first filter 10. The beam is then reflected by a reflector 11 and a dichroic mirror 13 in sequence to change the beam path. The beam is focused and coupled into a single optical fiber 6 by a third lens 12, and the single optical fiber 6 transmits the laser to the sample 2.

[0074] Step S4: After the sample 2 is excited by the laser, a Raman signal light is generated, and the single optical fiber 6 transmits the Raman signal light to the light splitting and beam combining lens group 7;

[0075] Step S5: The third lens 12 is used to shape the Raman signal light into a collimated light beam. The Raman signal light is sequentially filtered by the dichroic mirror 13 for primary filtering, the second filter 14 for secondary filtering, and then the Raman signal light is focused and coupled to the Raman signal collection optical fiber 8 by the second lens 15 for tertiary filtering.

[0076] Step S6: using the spectrometer 4 to collect and analyze the spectrum of the Raman signal light after the tertiary filtering.

[0077] In the embodiment of the present disclosure, when the Raman spectrum detection is performed on the sample 2, the laser 1 is started to emit laser, and the laser enters the light splitting and combining lens group 7 through the laser excitation optical fiber 5, and is shaped into a collimated light beam through the aspheric first lens 9. The collimated light beam is uniform parallel light. The collimated laser passes through the first filter 10 perpendicular to the optical path for filtering, thereby reducing the laser spectrum bandwidth and improving the accuracy of the excitation light wavelength. The filtered laser passes through a reflector 11 with a high reflection film on the surface and is placed at 45 degrees to the optical path. It is reflected to a short-wave reflection and long-wave transmission dichroic mirror 13 placed parallel to it. The laser reflected by the dichroic mirror 13 is focused by the aspheric third lens 12 and coupled into the single optical fiber 6. The single optical fiber 6 is driven to move in the laser propagation direction by the movable coupling member 16, so that the laser focused by the third lens 12 is efficiently coupled into the single optical fiber 6, and then the laser is transmitted to the sample 2 by the single optical fiber 6. According to different samples 2, single optical fibers 6 of different lengths and core diameters can be selected; after the sample 2 is excited by the laser, Raman signal light is generated, and the single optical fiber 6 collects the Raman signal light ( The Raman signal light passes through the single optical fiber 6 and enters the light splitting and combining lens group 7, and is shaped into a collimated light beam by the aspheric third lens 12. The collimated Raman signal light passes through the dichroic mirror 13 for primary filtering. Since the dichroic mirror 13 has a short-wave reflection function, part of the laser reflected light and Rayleigh scattered light are filtered out. The filtered Raman signal light passes through the second filter 14 perpendicular to the optical path for secondary filtering, and the laser reflected light and Rayleigh scattered light are secondary filtered. In addition, the Raman signal light after the secondary filtering is focused and coupled to the Raman signal collection optical fiber 8 by the second lens 15, and the Raman signal collection optical fiber 8 is an optical fiber etched with a fiber Bragg grating. The Raman signal collection optical fiber 8 performs three filtering on the Raman signal light after the secondary filtering, and filters the laser reflected light and Rayleigh scattered light three times. At the same time, it can filter out part of the Raman light generated by the single optical fiber 6 itself. Finally, after filtering out other light, the Raman signal collection optical fiber 8 transmits the clean Raman scattered light to the spectrometer 4 for spectrum collection and analysis.

[0078] Compared with the existing dual-fiber or multi-fiber integration method, the method for medical Raman spectroscopy detection effectively saves costs by arranging a single optical fiber at the output end of the Raman probe device, and excites samples and collects Raman signals through the single optical fiber, thereby achieving lightweight and simplification of the output end of the Raman probe. The use of a single optical fiber can ensure the position consistency of the Raman signal light of the excited sample and the collected sample, thereby improving the accuracy of the overall analysis. By arranging an optical fiber with a fiber Bragg grating at the collection end of the Raman probe device to perform a third filtering of the reflected light, Rayleigh scattering and other stray light returned by the excitation light, the signal crosstalk can be effectively blocked, the Raman signal generated by the single optical fiber itself can be reduced, the purity and singleness of the Raman signal collection can be achieved, the signal-to-noise ratio can be improved, and the accuracy and reliability of data analysis can be increased.

[0079] Those skilled in the art will readily appreciate other embodiments of the present disclosure after considering the specification and practicing the invention disclosed herein. This application is intended to cover any modification, use or adaptation of the present disclosure, which follows the general principles of the present disclosure and includes common knowledge or customary techniques in the art that are not disclosed in the present disclosure. The specification and examples are intended to be exemplary only, and the true scope and spirit of the present disclosure are indicated by the appended claims.

Claims

1. A device for medical Raman spectroscopy detection, characterized in that: The device comprises: A laser (1) for emitting laser light toward a sample (2); A Raman probe device (3) is configured to include a laser excitation optical path and a Raman signal collection optical path, wherein the laser excitation optical path is used to transmit the laser light emitted by the laser (1) to the sample (2), and the Raman signal collection optical path is used to collect the Raman signal light generated after the laser excites the sample (2); The spectrometer (4) is used to receive the Raman signal light from the Raman signal collection optical path and perform spectrum collection and analysis on the Raman signal light.

2. The device for medical Raman spectroscopy detection according to claim 1, characterized in that: The Raman probe device (3) comprises: A laser excitation optical fiber (5) for receiving laser light emitted by the laser (1); A single optical fiber (6) is used to transmit the laser to the sample (2) and collect Raman signal light generated after the laser excites the sample (2); A light splitting and beam combining lens group (7) is arranged between the laser excitation optical fiber (5) and the single optical fiber (6), and the light splitting and beam combining lens group (7) is used to shape and filter the laser light output by the laser excitation optical fiber (5), and to shape and filter the Raman signal light output by the single optical fiber (6); The Raman signal collecting optical fiber (8) is used to filter the Raman signal light output by the light splitting and beam combining lens group (7), and transmit the filtered Raman signal light to the spectrometer (4).

3. The device for medical Raman spectroscopy detection according to claim 2, characterized in that: The light splitting and beam combining lens group (6) comprises: A first lens (9), a first filter (10) and a reflector (11) are arranged in sequence along the direction in which the laser excitation optical fiber (5) outputs laser light, wherein the first lens (9) and the first filter (10) are perpendicular to the optical path, and the reflector (11) is arranged at an angle of 45 degrees to the direction opposite to the laser input direction; A dichroic mirror (13) is located on the reflection light path of the reflection mirror (11), the dichroic mirror (13) and the reflection mirror (11) are parallel to each other, a third lens (12) is vertically arranged on the reflection light path of the dichroic mirror (13), a second filter (14) and a second lens (15) are vertically arranged in sequence along the reverse direction of the reflection light path, the single optical fiber (6) is located on a side of the third lens (12) away from the dichroic mirror (13), and the Raman signal collection optical fiber (8) is located on a side of the second lens (15) away from the dichroic mirror (13); The reflection light path of the dichroic mirror (13) is parallel to the output direction of the Raman signal light.

4. The device for medical Raman spectroscopy detection according to claim 3, characterized in that: The laser excitation optical path comprises, in sequence, the first lens (9), a first filter (10), a reflector (11), a dichroic mirror (13), a third lens (12) and a single optical fiber (6); The Raman signal collection optical path comprises the single optical fiber (6), a third lens (12), a dichroic mirror (13), a second filter (14) and a second lens (15) in sequence; The single optical fiber (6), the third lens (12), and the dichroic mirror (13) are common components of the laser excitation optical path and the Raman signal collection optical path.

5. The device for medical Raman spectroscopy detection according to claim 3, characterized in that: The first lens (9) and the third lens (12) are both aspherical lenses; The surface of the reflector (11) is coated with a high reflection film.

6. The device for medical Raman spectroscopy detection according to claim 3, characterized in that: The diameter of the second lens (15) is greater than the diameters of the first lens (9) and the third lens (12).

7. The device for medical Raman spectroscopy detection according to claim 3, characterized in that: The dichroic mirror (13) is used to transmit the first wavelength and to reflect the second wavelength; The first wavelength is a long wave, and the second wavelength is a short wave.

8. The device for medical Raman spectroscopy detection according to claim 3, characterized in that: A coupling member (16) is provided at one end of the single optical fiber (6) close to the third lens (12), and the coupling member (16) can drive the single optical fiber (6) to move back and forth along the Raman signal light output direction.

9. The device for medical Raman spectroscopy detection according to claim 2, characterized in that: The Raman signal collection optical fiber (8) is configured as an optical fiber etched with a fiber Bragg grating.

10. A method for medical Raman spectroscopy detection, characterized in that: The method of performing Raman spectroscopy detection using the device for medical Raman spectroscopy detection according to any one of claims 1 to 9 comprises: Step S1: using a laser (1) to emit laser light; Step S2: using a laser excitation optical fiber (5) to transmit the laser light to the light splitting and beam combining lens group (7); Step S3: using a first lens (9) to shape the laser into a collimated beam, using a first filter (10) to filter the beam, then sequentially reflecting through a reflector (11) and a dichroic mirror (13) to change the beam path, focusing the beam through a third lens (12) and coupling it into a single optical fiber (6), and the single optical fiber (6) transmits the laser to the sample (2); Step S4: the sample (2) generates Raman signal light after being excited by the laser, and the single optical fiber (6) transmits the Raman signal light to the light splitting and beam combining lens group (7); Step S5: using a third lens (12) to shape the Raman signal light into a collimated light beam, the Raman signal light is sequentially filtered by a dichroic mirror (13) for primary filtering, filtered by a second filter (14) for secondary filtering, and then focused and coupled by a second lens (15) to a Raman signal collection optical fiber (8) for tertiary filtering; Step S6: using a spectrometer (4) to collect and analyze the spectrum of the Raman signal light after the three-time filtering.

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