Embryo biopsy and biopsy specimen tubing integrated device in cleavage stage and blastocyst stage

Abstract

The invention discloses a cleavage stage and blastocyst stage embryo biopsy and biopsy specimen tubing integrated device, relates to the technical field of biopsy specimen transfer and tubing instruments, and provides the following scheme that the cleavage stage and blastocyst stage embryo biopsy and biopsy specimen tubing integrated device comprises a biopsy vessel, and the left side wall of the top of the biopsy vessel is fixedly connected with a gripper displacement assembly; and a biopsy sample transfer gripper is arranged on the right side end face of the gripper displacement assembly. The placing assembly for placing the EP tube is arranged on the side edge of the biopsy vessel, the tube is directly installed under microscope observation after biopsy, time is saved, errors are avoided, the gripper displacement assembly and the biopsy sample transfer gripper are arranged on the side edge of the biopsy vessel, and by means of mutual cooperation of the gripper displacement assembly and the biopsy sample transfer gripper, the biopsy sample transfer efficiency is improved. According to the device, the biopsy sample tissue can be quickly and effectively grabbed and then put into the EP tube on the side edge, so that the efficiency and accuracy of embryo biopsy are improved, the tube loading time is saved, and the biopsy sample is successfully transferred.

Description

Technical Field

[0001] The invention relates to the technical field of biopsy specimen transfer and tube loading instruments, and in particular to an integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading. Background Art

[0002] Embryo biopsy refers to the removal of a portion of tissue for pathological examination in the early stages of pregnancy, a critical period of embryonic development, to determine whether the embryo is developing normally. Embryo biopsy is mainly to understand whether the embryo is developing normally. Embryo biopsy can be used to determine whether there are chromosomal abnormalities, single gene diseases, infectious diseases, etc. In daily operations, embryo biopsy is first performed using a biopsy dish. After the biopsy, the embryo is transferred out for cryopreservation. The biopsy specimen needs to be transferred to an EP tube using a transfer tube. This process has several major disadvantages. One is that the transfer is wrong and cannot correspond one-to-one with the embryo. The other is that the specimen is lost during the transfer, and it takes a long time. Summary of the invention

[0003] In view of the above background technology, it is proposed that the biopsy specimen needs to be transferred to an EP tube using a transfer tube. There is a risk of transfer error, failure to correspond one-to-one with the embryo, and loss of the specimen during transfer. Therefore, a technical solution is provided for an integrated device for biopsy of cleavage-stage and blastocyst-stage embryos and for loading biopsy specimens into tubes.

[0004] It includes a biopsy vessel, a gripper displacement assembly is fixedly connected to the left side wall of the top of the biopsy vessel, a biopsy sample transfer gripper is arranged on the right end surface of the gripper displacement assembly, and an EP centrifuge tube placement assembly is fixedly connected to the right surface of the biopsy vessel;

[0005] The biopsy vessel comprises a hard rubber pad, a glass biopsy vessel placed on the upper surface of the hard rubber pad, and a biopsy vessel end cap clamped on the upper port of the glass biopsy vessel, a positioning ring is fixedly connected to the top surface of the hard rubber pad, and the inner ring surface of the positioning ring contacts the bottom area of ​​the outer ring surface of the glass biopsy vessel;

[0006] The gripper displacement assembly includes a horizontally arranged transverse bottom plate and a vertically arranged vertical upright plate, two transverse electric slide rails are fixed on the top surface of the transverse bottom plate, transverse electric sliders are slidably arranged on the slideways of the transverse electric slide rails, and the top end surfaces of the transverse electric sliders are fixedly connected with support plates; two vertical electric slide rails are fixed on the right side surface of the vertical upright plate, vertical electric sliders are slidably arranged on the slideways of the vertical electric slide rails, and an extension plate is fixedly connected to the right side end surface of the vertical electric slider;

[0007] Specifically, the glass biopsy dish 13 is inserted into the protrusion 12, and the glass biopsy dish 13 is supported by the hard rubber pad 11. The biopsy tissue is put into the glass biopsy dish, and after the inspection is performed using a microscope, the EP tube cover at the upper port of the EP tube is opened, and then the horizontal electric slider on the horizontal bottom plate is used to drive the support plate to move to the right, driving the biopsy sample transfer gripper to move to the top of the glass biopsy dish, and then the vertical electric slider on the vertical electric slide rail drives the extension plate to move downward, so that the biopsy sample transfer gripper is close to the biopsy sample inside the glass biopsy dish.

[0008] The biopsy sample transfer gripper includes a fixed disc, a servo motor fixed on the upper surface of the fixed disc, and a lower disc located below the servo motor, wherein the output shaft of the servo motor is connected to a screw through a coupling, and the outer ring of the screw is provided with a movable plate, and the outer ring surface of the movable plate is hinged with 6-8 pulling arms in a circular array, and the bottom end of the pulling arm is hinged with a sample clamping arm, and the top of the pulling arm is hinged with the outer ring surface of the fixed disc; specifically, after the sample clamping arm is located around the biopsy sample, the screw is driven to rotate by the servo motor, thereby driving the movable plate to move downward, and at this time, under the pulling action of the movable plate and the pulling arm, the sample clamping arm is closed together along the hinge point until the sample clamping arm is closed and gently clamps the biopsy sample. Then, the biopsy sample transfer gripper is lifted upward and continues to move to the right through the gripper displacement assembly until the biopsy sample transfer gripper reaches the upper port position of the EP tube, and the biopsy sample is put into the EP tube through the sample clamping arm.

[0009] The EP centrifuge tube placement assembly includes an arc-shaped curved rod, a fixing ring fixed on the right end of the arc-shaped curved rod, and 6-8 plastic sheets fixedly connected to the bottom surface of the fixing ring in an annular array. EP tubes are inserted and placed inside the fixing ring and the plastic sheets.

[0010] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: a plurality of criss-crossing anti-slip grooves are provided on the bottom surface of the hard rubber pad, a protrusion is fixedly connected to the left surface of the hard rubber pad, and the right end face of the transverse bottom plate is fixedly connected to the left surface of the protrusion.

[0011] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: the middle area of ​​the top surface of the hard rubber pad is in contact with the bottom surface of the glass biopsy dish, and the right side of the top surface of the hard rubber pad is fixedly connected to the bottom end of the arc-shaped bent rod.

[0012] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: a microscope facing the biopsy sample inside the glass biopsy dish is provided on the front side of the hard rubber pad, and the biopsy sample tissue is loaded in the inner cavity of the hard rubber pad.

[0013] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: the plastic sheet is overall conical, the diameter of the top port of the plastic sheet is larger than the diameter of the bottom port, and the plastic sheets have notches between each other for the plastic sheets to deform inward or outward.

[0014] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: the outer ring surface of the EP tube is in close contact with the inner surface of the plastic sheet, and the top end of the EP tube is connected to an EP tube cap.

[0015] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: the transverse electric slider on the transverse electric slide rail moves linearly left and right synchronously, and baffles for controlling the travel of the transverse electric slider are fixed at both left and right ends of the transverse base plate.

[0016] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: the left side surface of the vertical stand is fixedly connected to the right side surface of the support plate, and the right end surface of the extension plate is fixedly connected to the left side end surface of the servo motor.

[0017] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: 6-8 U-shaped grooves arranged in a circular array are opened on the outer ring surface of the fixed disk, the top end of the pulling arm is inserted into the inside of the U-shaped groove and is rotatably connected via an axle pin; the interior of the movable plate is inlaid with a nut that matches the outer ring thread of the screw rod.

[0018] In the technical solution of the above-mentioned integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading, preferably: four guide posts penetrating the movable plate are fixed in a circular array on the top surface of the lower circular plate, and the top ends of the guide posts are fixedly connected to the bottom surface of the fixed circular plate.

[0019] It can be seen from the above technical scheme that the present invention provides an integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading. Compared with the prior art, the present invention has the following beneficial effects:

[0020] In the present invention, a placement component for placing the EP tube is arranged on the side of the biopsy dish, and the tube is directly loaded under microscope observation after biopsy, which saves time and avoids errors. In addition, a gripper displacement component and a biopsy sample transfer gripper are arranged on the side of the biopsy dish. The two cooperate with each other to achieve that the sample tissue after biopsy can be quickly and effectively grasped and then put into the EP tube on the side, thereby improving the efficiency and accuracy of embryo biopsy, saving tube loading time, and ensuring that the biopsy specimen is successfully transferred. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces and describes the drawings required for use in the embodiments of the present invention or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0022] Figure 1 It is a schematic diagram of the overall structure of the integrated device for loading and unloading a biopsy specimen;

[0023] Figure 2 is a schematic diagram of a biopsy vessel;

[0024] Figure 3 A schematic diagram of the EP centrifuge tube placement assembly;

[0025] Figure 4 is a schematic diagram of the gripper displacement assembly;

[0026] Figure 5 Schematic diagram of the biopsy sample transfer gripper.

[0027] Attached Figure 1 -Attached Figure 5 The corresponding relationship of the parts is as follows:

[0028] 1. Biopsy vessel; 11. Hard rubber pad; 12. Bump; 13. Glass biopsy vessel; 14. Biopsy vessel end cover; 15. Positioning ring; 2. Gripper displacement assembly; 21. Horizontal bottom plate; 22. Horizontal electric slide rail; 23. Support plate; 24. Vertical stand plate; 25. Vertical electric slide rail; 26. Vertical electric slider; 27. Extension plate; 28. Horizontal electric slider; 3. Biopsy sample transfer gripper; 31. Fixed disc; 32. U-shaped groove; 33. Screw rod; 34. Pulling arm; 35. Sample clamping arm; 36. Lower circular plate; 37. Guide column; 38. Movable plate; 39. Servo motor; 4. EP centrifuge tube placement assembly; 41. Arc-shaped bent rod; 42. Plastic sheet; 43. Fixed ring; 44. EP tube; 45. EP tube cover. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described below are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] In order to more clearly explain and illustrate the technical solution and implementation of the present invention, preferred specific embodiments for implementing the technical solution of the present invention are introduced below.

[0031] Embodiment: A preferred technical solution of an integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading:

[0032] The biopsy vessel 1 comprises a gripper displacement assembly 2 fixedly connected to the top left side wall of the biopsy vessel 1, a biopsy sample transfer gripper 3 is arranged on the right end surface of the gripper displacement assembly 2, and an EP centrifuge tube placement assembly 4 is fixedly connected to the right surface of the biopsy vessel 1;

[0033] The biopsy vessel 1 comprises a hard rubber pad 11, a glass biopsy vessel 13 placed on the upper surface of the hard rubber pad 11, and a biopsy vessel end cap 14 clamped on the upper port of the glass biopsy vessel 13. A positioning ring 15 is fixedly connected to the top surface of the hard rubber pad 11, and the inner ring surface of the positioning ring 15 contacts the bottom area of ​​the outer ring surface of the glass biopsy vessel 13.

[0034] The gripper displacement assembly 2 includes a horizontally arranged transverse bottom plate 21 and a vertically arranged vertical upright plate 24. Two transverse electric slide rails 22 are fixed on the top surface of the transverse bottom plate 21. Transverse electric slide rails 28 are slidably arranged on the slideways of the transverse electric slide rails 22. The top end surface of the transverse electric slide rail 28 is fixedly connected to a support plate 23.

[0035] Two vertical electric slide rails 25 are fixed on the right side surface of the vertical upright plate 24, and vertical electric sliders 26 are slidably arranged on the slideways of the vertical electric slide rails 25, and an extension plate 27 is fixedly connected to the right end surface of the vertical electric slider 26;

[0036] The biopsy sample transfer gripper 3 includes a fixed disc 31, a servo motor 39 fixed on the upper surface of the fixed disc 31, and a lower disc 36 located below the servo motor 39. The output shaft of the servo motor 39 is connected to a screw rod 33 through a coupling. A movable plate 38 is provided on the outer ring of the screw rod 33. Six to eight pulling arms 34 are hinged in a circular array on the outer ring surface of the movable plate 38. The bottom end of the pulling arm 34 is hinged to a sample clamping arm 35. The top end of the pulling arm 34 is hinged to the outer ring surface of the fixed disc 31.

[0037] The EP centrifuge tube placement assembly 4 includes an arc-shaped curved rod 41, a fixing ring 43 fixed on the right end of the arc-shaped curved rod 41, and 6-8 plastic sheets 42 fixedly connected to the bottom surface of the fixing ring 43 in a circular array. An EP tube 44 is inserted and placed inside the fixing ring 43 and the plastic sheet 42.

[0038] The bottom surface of the hard rubber pad 11 is provided with a plurality of crisscross anti-skid lines, the left surface of the hard rubber pad 11 is fixedly connected with a protrusion 12, and the right end surface of the transverse bottom plate 21 is fixedly connected with the left surface of the protrusion 12. The middle area of ​​the top surface of the hard rubber pad 11 contacts the bottom surface of the glass biopsy dish 13, the right side of the top surface of the hard rubber pad 11 is fixedly connected with the bottom end of the arc-shaped curved rod 41, the front side of the hard rubber pad 11 is provided with a microscope facing the biopsy sample inside the glass biopsy dish 13, and the inner cavity of the hard rubber pad 11 is loaded with biopsy sample tissue.

[0039] The plastic sheet 42 is tapered as a whole, the top port diameter of the plastic sheet 42 is larger than the bottom port diameter, and there are gaps between the plastic sheets 42 for the plastic sheets 42 to deform inward or outward. The outer ring surface of the EP tube 44 is in close contact with the inner surface of the plastic sheet 42, and the top of the EP tube 44 is connected to the EP tube cover 45. The transverse electric slider 28 on the transverse electric slide rail 22 is synchronously linearly moved left and right, and baffles for controlling the travel of the transverse electric slider 28 are fixed at both ends of the transverse bottom plate 21. The left surface of the vertical stand 24 is fixedly connected to the right surface of the support plate 23, and the right end face of the extension plate 27 is fixedly connected to the left end face of the servo motor 39.

[0040] The outer surface of the fixed disc 31 is provided with 6-8 U-shaped grooves 32 arranged in a circular array. The top end of the pulling arm 34 is inserted into the U-shaped groove 32 and is rotatably connected through an axle pin. The interior of the movable plate 38 is inlaid with a screw nut that matches the outer thread of the screw rod 33.

[0041] Four guide posts 37 penetrating the movable plate 38 are fixed in a circular array on the top surface of the lower circular plate 36 , and the top ends of the guide posts 37 are fixedly connected to the bottom surface of the fixed circular plate 31 .

[0042] According to the above-mentioned preferred technical solution, the workflow of the technical solution is described as follows:

[0043] Insert the glass biopsy dish 13 into the protrusion 12, and support the glass biopsy dish 13 with the hard rubber pad 11. Put the biopsy tissue into the glass biopsy dish 13, and after testing with a microscope, open the EP tube cover 45 at the upper end of the EP tube 44, and then use the horizontal electric slider 28 on the horizontal bottom plate 21 to drive the support plate 23 to move to the right, so as to drive the biopsy sample transfer gripper 3 to move to the top of the glass biopsy dish 13, and then drive the extension plate 27 to move downward through the vertical electric slider 26 on the vertical electric slide rail 25, so that the biopsy sample transfer gripper 3 is close to the biopsy sample inside the glass biopsy dish 13.

[0044] After the sample clamping arm 35 is located around the biopsy sample, the servo motor 39 drives the screw rod 33 to rotate, thereby driving the movable plate 38 to move downward. At this time, under the pulling action of the movable plate 38 and the pulling arm 34, the sample clamping arms 35 are closed together along the hinge points until the sample clamping arms 35 are closed and gently clamp the biopsy sample. Then, the biopsy sample transfer gripper 3 is lifted upward and continues to move to the right through the gripper displacement assembly 2 until the biopsy sample transfer gripper 3 reaches the upper port position of the EP tube 44, and the biopsy sample is put into the EP tube 44 through the sample clamping arm 35.

[0045] The present invention is not limited to the above-mentioned optimal implementation mode. Anyone should be aware that any structural changes made under the inspiration of the present invention, and any technical solutions that are the same or similar to the present invention, fall within the protection scope of the present invention. Finally, it should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the restrictive conditions that can be implemented in this application, so they have no technical substantive significance. Any structural modification, change in proportional relationship or adjustment of size, without affecting the effects that can be produced by this application and the purposes that can be achieved, should still fall within the scope that can be covered by the technical content disclosed in this application.

Claims

1. An integrated device for biopsy of cleavage-stage and blastocyst-stage embryos and for loading biopsy specimens into tubes, comprising a biopsy vessel (1), characterized in that: A gripper displacement assembly (2) is fixedly connected to the left side wall of the top of the biopsy vessel (1), a biopsy sample transfer gripper (3) is arranged on the right end surface of the gripper displacement assembly (2), and an EP centrifuge tube placement assembly (4) is fixedly connected to the right surface of the biopsy vessel (1); The biopsy vessel (1) comprises a hard rubber pad (11), a glass biopsy vessel (13) placed on the upper surface of the hard rubber pad (11), and a biopsy vessel end cover (14) clamped on the upper port of the glass biopsy vessel (13); a positioning ring (15) is fixedly connected to the top surface of the hard rubber pad (11), and the inner ring surface of the positioning ring (15) contacts the bottom area of ​​the outer ring surface of the glass biopsy vessel (13); The gripper displacement assembly (2) comprises a horizontally arranged transverse bottom plate (21) and a vertically arranged vertical upright plate (24); two transverse electric slide rails (22) are fixed on the top surface of the transverse bottom plate (21); transverse electric slide blocks (28) are slidably arranged on the slideways of the transverse electric slide rails (22); and the top end surfaces of the transverse electric slide blocks (28) are fixedly connected to support plates (23); Two vertical electric slide rails (25) are fixed on the right side surface of the vertical upright plate (24), vertical electric slide blocks (26) are slidably arranged on the slideways of the vertical electric slide rails (25), and an extension plate (27) is fixedly connected to the right side end surface of the vertical electric slide block (26); The biopsy sample transfer gripper (3) comprises a fixed disc (31), a servo motor (39) fixed on the upper surface of the fixed disc (31), and a lower disc (36) located below the servo motor (39); the output shaft of the servo motor (39) is connected to a screw rod (33) via a coupling; a movable plate (38) is provided on the outer ring of the screw rod (33); 6-8 pulling arms (34) are hingedly connected in a circular array on the outer ring surface of the movable plate (38); the bottom end of the pulling arm (34) is hingedly connected to a sample clamping arm (35); the top end of the pulling arm (34) is hingedly connected to the outer ring surface of the fixed disc (31); The EP centrifuge tube placement assembly (4) comprises an arc-shaped curved rod (41), a fixing ring (43) fixed on the right end of the arc-shaped curved rod (41), and 6-8 plastic sheets (42) fixedly connected to the bottom surface of the fixing ring (43) in an annular array, and an EP tube (44) is inserted and placed inside the fixing ring (43) and the plastic sheet (42).

2. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The bottom surface of the hard rubber pad (11) is provided with a plurality of crisscross anti-skid grooves, the left side surface of the hard rubber pad (11) is fixedly connected to a protrusion (12), and the right end surface of the transverse bottom plate (21) is fixedly connected to the left side surface of the protrusion (12).

3. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The middle area of ​​the top surface of the hard rubber pad (11) contacts the bottom surface of the glass biopsy dish (13), and the right side of the top surface of the hard rubber pad (11) is fixedly connected to the bottom end of the arc-shaped curved rod (41).

4. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: A microscope facing the biopsy sample inside the glass biopsy dish (13) is arranged on the front side of the hard rubber pad (11), and the biopsy sample tissue is loaded in the inner cavity of the hard rubber pad (11).

5. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The plastic sheet (42) is tapered as a whole, the diameter of the top port of the plastic sheet (42) is larger than the diameter of the bottom port, and the plastic sheets (42) are provided with notches for the plastic sheets (42) to deform inwardly or outwardly.

6. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The outer ring surface of the EP tube (44) is in close contact with the inner surface of the plastic sheet (42), and the top end of the EP tube (44) is connected to an EP tube cover (45).

7. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The transverse electric slide block (28) on the transverse electric slide rail (22) moves linearly left and right synchronously, and baffles for controlling the travel of the transverse electric slide block (28) are fixed at both left and right ends of the transverse bottom plate (21).

8. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The left side surface of the vertical stand plate (24) is fixedly connected to the right side surface of the support plate (23), and the right end surface of the extension plate (27) is fixedly connected to the left side surface of the servo motor (39).

9. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: The outer ring surface of the fixed disc (31) is provided with 6 to 8 U-shaped grooves (32) arranged in a circular array, and the top end of the pulling arm (34) is inserted into the inside of the U-shaped groove (32) and is rotatably connected via an axle pin; the inside of the movable plate (38) is inlaid with a nut that is compatible with the outer ring thread of the screw rod (33).

10. The integrated device for cleavage-stage and blastocyst-stage embryo biopsy and biopsy specimen tube loading according to claim 1, characterized in that: Four guide posts (37) penetrating the movable plate (38) are fixed in an annular array on the top surface of the lower circular plate (36), and the top ends of the guide posts (37) are fixedly connected to the bottom surface of the fixed circular plate (31).

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