Composition containing guaiac extract for improving acne in women during menstruation and application thereof

Abstract

The present invention relates to a composition for improving acne in women during menstruation containing guaiac extract and its application, the composition is composed of five components of mango leaf extract, myrtle fruit extract, 10 hydroxydecanoic acid, Rehmannia glutinosa extract and guaiac extract, and these five components have a synergistic effect, can effectively inhibit lipid oxidation and promote the migration of skin keratinocytes, play the effect of dredging sebaceous glands and preventing the generation of acne; and can also inhibit 5 α reductase to reduce the stimulation of male hormones, thereby reducing the situation of increased sebum secretion due to menstrual hormone fluctuations; and can inhibit Propionibacterium acnes, play a role in preventing acne generation and improving existing acne; in addition, the composition of the present invention has a fresh smell, can soothe the irritable mood of women during menstruation, and indirectly improve skin condition. Therefore, applying the composition to cosmetics can improve facial acne in women with acne during menstruation and soothe the irritable mood of women during menstruation.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a composition containing a guaiac extract for improving acne in menstruating women and an application thereof. Background Art

[0002] Menstrual acne is a common problem for many women during their menstrual cycle. It is characterized by a significant increase in the number of acne breakouts from the week before menstruation to the onset of menstruation. There are various types of menstrual acne, primarily including inflammatory acne and comedonal lesions, and it typically resolves within a week after menstruation ends. During the luteal phase of the menstrual cycle, increased estrogen and androgen secretions lead to increased sebum production. Reports indicate that during this period, the sebaceous ducts in the skin narrow, allowing sebum to accumulate, leading to the formation and aggravation of acne. Furthermore, a decrease in the skin's barrier function before menstruation can also exacerbate acne due to increased bacterial growth. Mood swings during menstruation are also a significant factor in exacerbating acne. Hormonal fluctuations during menstruation often cause anxiety, stress, or depression in women, which not only impacts their mental state but can also lead to increased sebum production and worsening acne. Therefore, the difficulty in treating menstrual acne lies in its hormonal dependence and the interplay of emotional factors, complicating targeted treatment options. Unstable hormone levels, structural changes in the sebaceous glands, and mood swings can affect their function, leading to persistent acne. Furthermore, when women face premenstrual discomfort and emotional challenges, they may neglect their skincare routine, further exacerbating skin problems.

[0003] The treatment of menstrual acne is of great significance. First, it not only affects skin health but also has a direct impact on mental health and self-confidence. The anxiety and low self-esteem caused by acne in women often affect their daily lives and social activities. Therefore, timely and effective treatment can improve skin condition and reduce psychological burden. Currently, products on the market do not address the need for menstrual acne. Most acne-removing products simply use powerful ingredients such as salicylic acid, glycolic acid, or zinc oxide to treat acne, which can aggravate the unstable state of menstrual skin and the stinging pain can also increase the anxiety of menstruating women. Therefore, there is a need to develop acne care compositions specifically for menstruating women. Summary of the Invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a composition containing guaiac extract for improving acne in menstrual women and its application.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a composition for improving acne in women during menstruation containing guaiac wood extract, the composition being composed of the following components: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract, wherein the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (0.1-5):(0.1-5):(0.01-1):(0.1-3):(0.1-3).

[0007] Mango leaf extract has been shown to significantly inhibit sebum oxidation in women experiencing menstrual acne. During menstruation, fluctuating hormone levels in women can lead to increased sebum production, which can accumulate in pores and cause breakouts. Through its potent antioxidant properties, mango leaf extract effectively reduces sebum oxidation, lowers sebaceous gland activity, and thus inhibits acne formation, offering a natural skincare treatment option for women experiencing menstrual acne.

[0008] Myrtle fruit extract plays an important role in anti-inflammatory, soothing, and maintaining a balanced skin microbiome. Period acne is often accompanied by skin inflammation and the proliferation of Propionibacterium acnes, a bacterium that harms sebaceous glands. Myrtle fruit extract reduces skin inflammation through anti-inflammatory mechanisms and inhibits harmful P. acnes subtypes IA1 and IA2, reducing damage to sebaceous glands. Furthermore, it helps balance the skin microbiome, preventing the overgrowth of undesirable bacteria, further improving skin condition.

[0009] 10-Hydroxydecanoic acid, also known as royal jelly acid, is a unique fatty acid extracted from royal jelly. It possesses antibacterial, anti-inflammatory, and anti-aging properties. Its ability not only refines pores and smoothes skin, but also reduces oil production and inhibits overactive sebaceous gland cells, achieving a dual effect: improving skin texture and reducing breakouts.

[0010] Rehmannia root extract inhibits 5-alpha reductase activity, reducing the effects of androgens and thus suppressing acne caused by hormone fluctuations. Its anti-inflammatory properties also help alleviate inflammatory responses caused by hormone fluctuations, further promoting skin health.

[0011] Guaiac wood extract also plays a significant role in soothing and anti-inflammatory properties. Its unique aromatic botanical ingredients not only soothe skin but also help women achieve a balanced mind and body during menstruation by regulating their moods. This mood-relief effect indirectly improves skin conditions by alleviating the increased emotional stress associated with menstrual fluctuations.

[0012] In summary, the composition of the present invention is composed of five ingredients: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia root extract, and guaiac extract. These five ingredients work synergistically to significantly inhibit the production of sebum oxidation markers (lipid peroxides LPO). Because sebum secretion is high during menstruation, oxidized sebum is more likely to clog the sebaceous glands, which is a challenge to the sebaceous glands, which are already narrow during menstruation. Therefore, anti-sebum oxidation can help unclog the sebaceous glands and prevent acne; promote the migration of skin keratinocytes; and have a significant inhibitory effect on 5-α reductase, which can greatly reduce the stimulation of male hormones. In addition, the composition can also inhibit Propionibacterium acnes and regulate mood. Therefore, the application of this composition to cosmetics can improve facial acne in female acne subjects during menstruation and relieve the irritability of women during menstruation.

[0013] Preferably, the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (1-3): (1-2): (0.01-0.5): (0.5-3): (1-3).

[0014] Preferably, the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3).

[0015] In a second aspect, the present invention provides the use of the composition containing the guaiac extract for improving acne in menstruating women in the preparation of skin care products.

[0016] Preferably, the skin care product is at least one of lotion, emulsion, cream, mask, essence or spray, and the added amount of the composition is 0.5%-5% of the total weight of the skin care product.

[0017] In a third aspect, the present invention provides an essence comprising the following raw materials in weight percentage: 0.5%-5% of the composition for improving acne in menstrual women containing guaiac extract in the first aspect, 0.05%-0.3% of a thickener, 0.5%-5% of a moisturizer, 0.5%-3% of a preservative, 0.01%-0.3% of a pH adjuster and 0.01%-1% of a fragrance, with the balance being deionized water.

[0018] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol.

[0019] Preferably, the moisturizing agent includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, hydrolyzed sodium hyaluronate, 1,2-butylene glycol, glycerol, budding pullulan polysaccharide, and ceramide. More preferably, glycerol, 1,2-butylene glycol, and hydrolyzed sodium hyaluronate are used.

[0020] Preferably, the pH regulator comprises at least one of arginine, tromethamine, and disodium EDTA, more preferably arginine.

[0021] Preferably, the preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol, and p-hydroxyacetophenone, more preferably 1,2-hexanediol and p-hydroxyacetophenone.

[0022] In a fourth aspect, the present invention provides a method for preparing the essence of the third invention, comprising the following steps:

[0023] S1. Mix a moisturizer, a thickener, and a portion of deionized water, homogenize at 75-85°C, cool to 55-65°C, add a preservative, and stir to obtain a mixture;

[0024] S2. When the temperature drops to 35-45° C., add the components of the composition and the remaining deionized water to the mixture obtained in step S1, stir evenly, and finally add a pH regulator to adjust the pH to obtain the essence.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The composition of the present invention is composed of five ingredients: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia root extract, and Guaiac wood extract. These five ingredients have a synergistic effect, effectively inhibiting lipid oxidation and promoting the migration of skin keratinocytes, thereby unblocking sebaceous glands and preventing acne. They also inhibit 5-α-reductase to reduce androgen stimulation, thereby reducing the increase in sebum secretion caused by menstrual hormone fluctuations. They also inhibit Propionibacterium acnes, preventing the development of acne and improving existing acne. Furthermore, the composition of the present invention has a pleasant fragrance that can soothe the irritability of women during menstruation, indirectly improving skin condition. Therefore, the application of this composition in cosmetics can improve facial acne in women with acne during menstruation and relieve the irritability of women during menstruation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The VISA graphs of the skin of the subjects who used the essences of Application Example 1 and Comparative Application Example 6 were tested on day 0 and day 28. DETAILED DESCRIPTION

[0028] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0029] The sources of the components used in the following examples and comparative examples are as follows:

[0030] Mango leaf extract: manufactured by Givaudan, brand name Mangixyl TM ;

[0031] Myrtle fruit extract: The manufacturer is France Green Natural Personal Care Products Co., Ltd., the brand is

[0032] 10-Hydroxydecanoic acid: manufactured by Beijing Dongfang Miaosen Biotechnology Co., Ltd.

[0033] Rehmannia root extract: manufacturer is Youran Biotechnology Co., Ltd.

[0034] Guaiac wood extract: The manufacturer is Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0035] Unless otherwise specified, other materials, reagents, etc. used in the Examples and Comparative Examples can be obtained from commercial sources.

[0036] Example 1

[0037] A composition for improving acne in women during menstruation, containing guaiac extract, comprises mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 2:1.8:0.07:0.6:2.6, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0038] Example 2

[0039] A composition for improving acne in women during menstruation containing a guaiac wood extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract in a weight ratio of 1.5:2:0.1:0.5:3, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is 100 parts.

[0040] Example 3

[0041] A composition for improving acne in women during menstruation, containing guaiac extract, comprises mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 2.5:1.5:0.05:1:2.5, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0042] Example 4

[0043] A composition for improving acne in women during menstruation containing a guaiac wood extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract in a weight ratio of 1:2; 0.01:3:1, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is 100 parts.

[0044] Example 5

[0045] A composition for improving acne in women during menstruation containing a guaiac wood extract, comprising mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract in a weight ratio of 3:1:0.5:0.5:3, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is 100 parts.

[0046] Example 6

[0047] A composition for improving acne in women during menstruation, containing guaiac extract, comprises mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 0.1:5:0.01:0.1:3, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0048] Example 7

[0049] A composition for improving acne in women during menstruation, containing guaiac extract, comprises mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract in a weight ratio of 5:0.1:1:3:0.1, wherein the total weight of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is 100 parts.

[0050] Comparative Example 1

[0051] The difference between Comparative Example 1 and Example 1 is that the total weight of the composition remains unchanged, mango leaf extract is not added, and a composition of myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract in a weight ratio of 1.8:0.07:0.6:2.6 is used to make up the missing amount.

[0052] Comparative Example 2

[0053] The difference between Comparative Example 2 and Example 1 is that the total weight of the composition remains unchanged, myrtle fruit extract is not added, and a composition of mango leaf extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract in a weight ratio of 2:0.07:0.6:2.6 is used to make up the missing amount.

[0054] Comparative Example 3

[0055] The difference between Comparative Example 3 and Example 1 is that the total weight of the composition remains unchanged, 10-hydroxydecanoic acid is not added, and a composition of mango leaf extract, myrtle fruit extract, rehmannia root extract and guaiac extract in a weight ratio of 2:1.8:0.6:2.6 is used to make up the missing amount.

[0056] Comparative Example 4

[0057] The difference between Comparative Example 4 and Example 1 is that the total weight of the composition remains unchanged, Rehmannia root extract is not added, and a composition of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid and guaiac extract in a weight ratio of 2:1.8:0.07:2.6 is used to make up the missing amount.

[0058] Comparative Example 5

[0059] The difference between Comparative Example 5 and Example 1 is that the total weight of the composition remains unchanged, no guaiac extract is added, and a composition of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid and Rehmannia glutinosa extract in a weight ratio of 2:1.8:0.07:0.6 is used to make up the missing amount.

[0060] Test Example 1: Lipid oxidation inhibition rate test

[0061] Lipid peroxides (LPO) are oxidation products produced by the reaction of lipids with reactive oxygen species and are often considered a marker of cell membrane damage and oxidative stress. In the pathogenesis of acne, LPO production is closely linked to inflammation, sebaceous gland dysfunction, and bacterial infection. High LPO levels typically reflect increased oxidative stress within the skin, which in turn triggers an exacerbated inflammatory response. This is particularly true in the skin of menstruating women, where not only are oxidized lipids more likely to form, but sebaceous glands become narrower, making them more susceptible to blockage and leading to the development and exacerbation of acne. Therefore, LPO levels can serve as an important indicator for assessing acne severity.

[0062] The compositions for improving acne in women during menstruation containing the guaiac extract of Examples 1-7 and the compositions of Comparative Examples 1-5 were tested according to the following steps. The results are shown in Table 1.

[0063] Human sebaceous gland cells SZ95 were revived under the following test conditions: incubator temperature 37±1°C, humidity 90±5%, and carbon dioxide 5±1%. Cells were cultured and treated according to grouping, and then tested. The specific test was the lipid peroxide (LPO) content. The test method is as follows:

[0064] (1) Recovery: The cell suspension was seeded into a 96-well cell culture plate at a density of 2000 cells per well. 100 μL of DMEM high-glucose medium containing 10% fetal bovine serum and 1% double-antibody solution (Gibco) was added to each well and cultured for 24 h.

[0065] (2) After the cells were revived, the original culture medium was discarded, 100 μL of culture medium containing 10 mM hydrocortisone was added to the control group, and 100 μL of culture medium containing 0.5 wt% of the composition + 10 mM hydrocortisone was added to each of the 12 sample groups, and the culture was continued for 24 h;

[0066] (3) Lipid peroxide (LPO) detection: The lipid peroxide (LPO) ELISA kit was used to detect the LPO content of the control group and the LPO content of each sample group. The inhibitory effect of the sample on LPO was expressed as the LPO inhibition rate, which was calculated as follows:

[0067] LPO inhibition rate (%) = (LPO content in control group - LPO content in sample group) / LPO content in control group × 100%.

[0068] Table 1 LPO inhibition rate data of each group

[0069]

[0070]

[0071] From the data of Example 1 and Comparative Examples 1-5 in Table 1, it can be seen that the composition of Example 1, which is a compound of five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia root extract and Guaiac wood extract, has a significantly improved LPO inhibition rate compared with the compositions of Comparative Examples 1-5, indicating that the five ingredients of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia root extract and Guaiac wood extract can synergistically improve the effect of dredging sebaceous glands and preventing acne.

[0072] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3), the composition is more effective in reducing sebum secretion and unblocking sebaceous glands.

[0073] Test Example 2: Antibacterial Rate Test

[0074] Acne in menstruating women is primarily related to hormonal fluctuations, particularly a decrease in estrogen. These hormonal changes increase sebaceous gland secretion, creating a favorable environment for the growth of Propionibacterium acnes. Furthermore, these bacteria utilize the oil in the skin to thrive and multiply, producing various inflammatory factors that trigger localized inflammation and worsen acne symptoms.

[0075] The compositions of Examples 1-7 and the compositions of Comparative Examples 1-5 were tested according to the following steps. The results are shown in Table 2.

[0076] Experimental Method: Liquid thioglycollate medium (FT medium) was prepared, and Propionibacterium acnes was inoculated into the medium. The culture was then incubated at 37°C for 48 hours to form a bacterial solution. Each composition was then prepared with DMSO to a 1% by volume sample solution for later use. 180 μL of the bacterial solution was then added to a 96-well plate, followed by 40 μL of each sample solution to create a sample group. A control group (a bacterial solution without sample solution) was also set up. After incubation for 24 hours, the OD value of each well was measured using a microplate reader. A lower OD value indicates clearer liquid, lower bacterial activity, and greater antibacterial effect of the composition. The inhibition rate was then calculated according to the following formula: Inhibition rate (%) = (control group OD value - sample group OD value) / control group OD value × 100%; the inhibition rate data are shown in Table 2.

[0077] Table 2 Inhibition rate of each sample solution on Propionibacterium acnes

[0078] Group Inhibition rate of Propionibacterium acnes / % Example 1 99.5 Example 2 98.0 Example 3 97.8 Example 4 97.2 Example 5 96.6 Example 6 90.6 Example 7 89.4 Comparative Example 1 31.0 Comparative Example 2 31.4 Comparative Example 3 32.6 Comparative Example 4 32.9 Comparative Example 5 31.8

[0079] From the data of Example 1 and Comparative Examples 1-5 in Table 2, it can be seen that the composition of Example 1, which is a compound of five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract, has a significantly improved inhibition rate against Propionibacterium acnes compared to the compositions of Comparative Examples 1-5, indicating that the five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract can synergistically inhibit Propionibacterium acnes, thereby preventing the occurrence of acne and improving existing acne.

[0080] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3), the composition has a better inhibitory effect on Propionibacterium acnes.

[0081] Test Example 3: Test of 5-α-reductase inhibition rate

[0082] 5-α-reductase is an enzyme that promotes the conversion of testosterone into dihydrotestosterone (DHT), which is closely related to the overactivity of the sebaceous glands. During menstruation, the estrogen level in women decreases and the androgen level increases relatively, leading to increased secretion of the sebaceous glands, thereby triggering the occurrence of acne. This change in hormone levels makes the skin more susceptible to the influence of bacteria such as Propionibacterium acnes, causing hair follicle blockage and inflammatory response. Therefore, inhibiting the activity of 5-α-reductase can effectively reduce the production of DHT, thereby reducing sebum secretion and inhibiting the inflammatory response. Detecting the inhibitory activity of the composition on 5-α-reductase can not only regulate the secretion state of the sebaceous glands, but also reduce the greasy feeling of the skin and the formation of acne. In addition, reducing the production of DHT can also reduce acne-related inflammation, promote skin healing and improve overall skin quality. Therefore, for women during menstruation, inhibiting 5-α-reductase to improve the treatment of acne is of great significance.

[0083] The compositions of Examples 1-7 and the compositions of Comparative Examples 1-5 were tested according to the following steps. The results are shown in Table 3.

[0084] Experimental method: Each composition was prepared with DMSO to prepare a sample solution with a volume ratio of 10%.

[0085] (1) Sample tube: Add 1 mL of each group of example and comparative example sample solution, 1 mL of enzyme solution, 1 mL of NADPH solution and 1 mL of testosterone solution to the test tube, shake gently to obtain a mixed liquid, and use a pipette to take 200 μL of the mixed liquid into a 96-well microplate. Make three replicates for each sample, place it in a microplate reader for detection, and measure the absorbance value at 340 nm, which is the first measured value A 样品0 After the sample was incubated at 37°C for 20 minutes, it was placed in a microplate reader for detection and the absorbance value at 340 nm was measured as the second measurement value A. 样品1 The enzyme solution is 50 U of 5-α reductase diluted with pH 6.8 PBS buffer; the NADPH solution is a 2 mM solution of NADPH prepared with pH 6.8 PBS; and the testosterone solution is a 0.83 nM solution of testosterone prepared with a 75% ethanol solution.

[0086] (2) Enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution to a test tube, shake gently to obtain a mixed liquid, and use a pipette to take 200 μL of the mixed liquid into a 96-well microplate. Make three replicates for each sample, place it in a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A. 酶0 After the sample was incubated at 37°C for 20 minutes, it was placed in a microplate reader for detection and the absorbance value at 340 nm was measured as the second measurement value A. 酶1 At the same time, blank and positive control tests were performed. The blank control group was prepared by replacing the sample solution in the sample tube with an equal amount of deionized water, and the positive control group was prepared by replacing the sample solution in the sample tube with 1 mL of a 25 g / L dutasteride solution using DMSO as the solvent. The steps were the same as above.

[0087] 5-α reductase inhibition rate (%) = [1-(A 样品0 -A 样品1 ) / (A 酶0 -A 酶1 )]×100%

[0088] Table 3 Data on the inhibition rate of 5-α reductase in each group of samples

[0089]

[0090]

[0091] From the data of Example 1 and Comparative Examples 1-5 in Table 3, it can be seen that the inhibition rate of the composition of Example 1, which is a compound of five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract, on 5-α-reductase is significantly improved compared with the compositions of Comparative Examples 1-5, indicating that the five ingredients including mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract can synergistically inhibit 5-α-reductase to reduce the stimulation of androgens, thereby reducing the increase in sebum secretion due to menstrual hormone fluctuations.

[0092] Combined with the data of Examples 1-7, it can be seen that when the weight ratio of mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia glutinosa extract and Guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3), the composition has a better inhibitory effect on 5-α reductase.

[0093] Application Examples 1-7 and Comparative Application Examples 1-5

[0094] The compositions of Examples 1-7 and Comparative Examples 1-5 were added to the essence at a concentration of 5 wt %, respectively, to obtain the essences of Application Examples 1-7 and Comparative Application Examples 1-5. The formulas are shown in Table 4.

[0095] The preparation method of the essence of Application Examples 1-7 and Comparative Application Examples 1-5 comprises the following steps:

[0096] The preparation method of the essence comprises the following steps:

[0097] S1. Mix the moisturizer, thickener and 1 / 2 amount of deionized water, homogenize at 80°C, cool to 60°C, add the preservative, and stir to obtain a mixture;

[0098] S2. When the temperature drops to 40° C., the components of the composition and the remaining deionized water are added to the mixture obtained in step S1 and stirred evenly. Finally, a pH regulator is added to adjust the pH to 6 to obtain the essence.

[0099] Table 4 Cream formulas of application examples 1-7 and comparative application examples 1-5

[0100]

[0101]

[0102] Comparative Application Example 6

[0103] In comparative application example 6, the essence was not added with the composition, and an equal amount of deionized water was used instead of the composition. The preparation method was the same as that of application example 1.

[0104] Test Example 4

[0105] Essence efficacy test

[0106] The specific testing method is as follows: 65 female subjects who are prone to acne during menstruation were screened by completing a questionnaire and randomly divided into 13 groups of 5 participants each. The subjects had obvious acne, blackheads, and papules. The subjects used the product on their entire face for 28 days, including their menstrual period. Every morning and evening, after cleansing and moisturizing, the subjects applied 1g of the essence from Application Examples 1-7 and Comparative Application Examples 1-6 to their face, massaging until absorbed. The application could be repeated on acne-prone areas. The entire face was used once in the morning and evening, and a follow-up visit was conducted after 28 days.

[0107] (1) Objective numerical test: Subjects were tested on day 0 and 28 after the test. On the day of the test, the subjects washed their faces with water without applying any products and sat quietly in an air-conditioned room at a temperature of 21±1°C and a humidity of 50±10% for 20 minutes. The improvement of facial acne indicators was quantified and photographed using instruments, including acne counting (calculating the total number of comedones and papules, using VISIA and doctor evaluation), and measuring the color of facial erythema with an MX 18 probe.

[0108] The serum's effectiveness in clearing and soothing acne was assessed by measuring the improvement in acne count and facial erythema. This was calculated as follows: improvement in objective values ​​before and after use = |mean before use - mean after use| / mean before use × 100%. See Table 5 for the data.

[0109] (2) Subjective numerical test: The subjects' improvement of facial acne and anxiety was tested by self-assessment questionnaires, which were completed on day 28. The questionnaires are as follows:

[0110] Questionnaire 1: Improvement of facial acne during menstruation by using serum;

[0111] No improvement: 0 points, slight improvement: 1 point, general improvement: 2 points, effective improvement: 3 points, great improvement: 4 points

[0112] Questionnaire 2: Relief of anxiety after using the serum;

[0113] No relief: 0 points, slight relief: 1 point, moderate relief: 2 points, effective relief: 3 points, and great relief: 4 points. Each subject's improvement score for menstrual acne and anxiety after 28 days was recorded, as shown in Table 4. The mean score for each group represents the serum's effectiveness in improving perceived acne and anxiety.

[0114] Table 5 Efficacy test data of each group of samples

[0115] Group Acne improvement rate / % Erythema improvement rate / % Acne improvement score Anxiety improvement score Application Example 1 99.6 98.9 3.4 3.6 Application Example 2 89.8 87.3 2.6 2.4 Application Example 3 93.3 94.4 2.4 2.8 Application Example 4 94.2 95.8 2.4 2.4 Application Example 5 97.2 96.1 2.6 2.4 Application Example 6 97.7 96.8 1.8 1.6 Application Example 7 98.3 97.6 1.8 1.4 Comparative Application Example 1 31.3 30.9 1.0 0.8 Comparative Application Example 2 31.3 32.0 0.8 1.0 Comparative Application Example 3 30.2 30.2 0.6 0.8 Comparative Application Example 4 30.9 31.3 0.8 1.0 Comparative Application Example 5 28.8 31.4 1.0 0.6 Comparative Application Example 6 2.3 1.2 0.4 0.2

[0116] As shown in Table 5, the essence of the present invention contains a composition composed of five ingredients: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, Rehmannia root extract, and Guaiac wood extract. These five ingredients have a synergistic effect, which makes the essence have better acne-removing and soothing effects.

[0117] Depend on Figure 1 As can be seen, after 28 days of use, the red area of ​​the VISA chart on the skin of the subjects tested with the essence of Application Example 1 was significantly reduced, indicating that the essence can significantly reduce skin inflammation and significantly improve acne. In contrast, the essence of Application Example 6, which does not contain the composition, has a less pronounced anti-inflammatory effect. This shows that the application of the composition of the present invention in cosmetics can improve facial acne in women with menstrual acne.

[0118] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition containing guaiac extract for improving acne in women during menstruation, characterized in that: The invention is composed of the following components: mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract, wherein the weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (0.1-5): (0.1-5): (0.01-1): (0.1-3): (0.1-3).

2. The composition for improving acne in women during menstruation containing the guaiac extract according to claim 1, wherein: The weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac extract is (1-3): (1-2): (0.01-0.5): (0.5-3): (1-3).

3. The composition for improving acne in women during menstruation containing the guaiac extract according to claim 1, wherein: The weight ratio of the mango leaf extract, myrtle fruit extract, 10-hydroxydecanoic acid, rehmannia root extract and guaiac wood extract is (1.5-2.5): (1.5-2): (0.05-0.1): (0.5-1): (2.5-3).

4. Use of the composition for improving acne in women during menstruation containing the guaiac extract according to any one of claims 1 to 3 in the preparation of skin care products.

5. Use of a composition for improving acne in women during menstruation containing a guaiac extract according to claim 4 in the preparation of a skin care product, characterized in that: The skin care product is at least one of lotion, emulsion, cream, facial mask, essence or spray, and the added amount of the composition is 0.5%-5% of the total weight of the skin care product.

6. An essence, characterized in that: The raw materials include the following weight percentages: 0.5%-5% of the composition for improving acne in menstruating women containing a guaiac extract according to any one of claims 1 to 3, 0.05%-0.3% of a thickener, 0.5%-5% of a moisturizer, 0.5%-3% of a preservative, 0.01%-0.3% of a pH adjuster, and 0.01%-1% of a fragrance, with the balance being deionized water.

7. The essence according to claim 6, characterized in that The raw material is selected from at least one of (a) to (d): (a) the thickener comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, sclerotium gum, and cetyl alcohol; (b) the moisturizing agent comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, hydrolyzed sodium hyaluronate, 1,2-butylene glycol, glycerin, budding pullulan polysaccharide, and ceramide; (c) the pH adjusting agent comprises at least one of arginine, tromethamine, and EDTA-disodium; (d) The preservative includes at least one of 1,2-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone.

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