Total cholesterol detection compound enzyme liquid, detection test paper and detection system

By using a complex enzyme solution containing enzymes such as cholesterol oxidase and cholesterol esterase, combined with electrochemical methods, the problem of long detection time in the prior art is solved, and a rapid and accurate detection effect is achieved.

CN120118970APending Publication Date: 2025-06-10NANJING EAGLENOS CO LTD
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Patent Information

Application Number
CN202311680418.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2023-12-08
Publication Date
2025-06-10

AI Technical Summary

Technical Problem

The prior art takes a long time to detect total cholesterol, and cannot achieve fast and accurate detection.

Method used

A complex enzyme solution is provided, including cholesterol oxidase, cholesterol esterase, peroxidase, electronic mediator, surfactant composition, etc., and the rapid detection of total cholesterol is achieved through electrochemical methods.

Benefits of technology

Fast, sensitive and accurate detection of total cholesterol is achieved, and results can be obtained in only 15-25 seconds, and sample size and detection time are reduced.

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Abstract

The invention relates to the technical field of biochemistry, in particular to compound enzyme liquid, detection test paper and a detection system for total cholesterol detection. The compound enzyme liquid for total cholesterol detection comprises cholesterol oxidase, cholesterol esterase, peroxidase, an electronic mediator, a surfactant composition, a thickening agent, a protective agent, metal inorganic salt and a buffer solution. The surfactant composition comprises one or a combination of at least two of surfactants with the HLB being larger than 23 and one or a combination of at least two of surfactants with the HLB being 12-14. The compound enzyme liquid can be used for detecting total cholesterol through an electrochemical method, and is high in detection speed, high in sensitivity and high in accuracy.
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