Application method of suspension composition for preventing and treating cucumber vegetable leafminers
By mixing amine and cyanidaamide and adding bio-stimulators to make a suspended composition, the existing pesticides have been solved, and the effect of rapid killing of pests and protecting crops is achieved.
Patent Information
- Application Number
- CN202311762443.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-20
- Publication Date
- 2025-06-20
AI Technical Summary
When preventing and controlling cucumber American flies, existing pesticides have poor permeability and dispersion, making it difficult to quickly pass through the crop epidermis and directly reach the pests, causing damage to the site, and the insecticide speed is slow.
Two agents with completely different mechanisms of action are mixed and bio-supramide, and bio-supramide are added to make a suspended composition, and the application is performed in the early stages of the occurrence of cucumber American supra by stem and leaf spraying.
This method can quickly pass through the crop epidermis and directly reach the harmful part of the squid fly. Through strong internal suction and conduction, it can quickly kill the squid fly larvae, protect new leaves from harm, and resist abiotic stress of crops, improve soil quality, prolong crop fruiting period, and slow down the occurrence of soil-borne diseases.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of pesticides, and particularly relates to an application method of a suspension composition for preventing and controlling cucumber leafminers. Background Art
[0002] Cyromazine is a low-toxic insecticide of the insect growth regulator class, which has very strong selectivity and is mainly active against Diptera insects. Its mechanism of action is to cause morphological deformation of Diptera insect larvae and pupae, and incomplete or inhibited emergence of adults. The drug has contact and stomach poisoning effects, and has strong systemic conductivity, a long lasting effect, but a slow action rate. Cyromazine has no toxic side effects on humans and animals, and is safe for the environment.
[0003] Cyantraniliprole is the second-generation nicotinic acid receptor inhibitor insecticide developed by DuPont after chlorantraniliprole. Compared with chlorantraniliprole, cyanantraniliprole has a broader insecticide spectrum and can control not only Lepidoptera pests, but also Hemiptera, Coleoptera and Diptera pests.
[0004] The main leafminers are the American leafminer and the South American leafminer. The American leafminer is more common in my country and often occurs on cucumbers, causing great harm. After the cucumber leafminer eggs hatch in the leaves, the larvae will eat the mesophyll tissue in the leaves. The symptoms of the cucumber leaves after being eaten are very obvious, and "ghostly" insect tunnels will appear on the leaves. In the later stages of cucumber leafminer damage, the leaves will turn yellow and fall off, and in severe cases, the plants will die.
[0005] The applicant mixed two agents with completely different mechanisms of action and added a biological synergist, which can effectively control cucumber leafminers, effectively solve the problems of poor permeability and dispersibility of pesticides, quickly penetrate the crop epidermis, directly reach the damaged parts of leafminers, and quickly kill leafminer larvae through strong systemic conduction, protecting new leaves from damage. It can also resist abiotic stress on crops, timely and effectively alleviate the stress of drought, waterlogging, frost and fertilizer damage, improve soil quality, purify soil, extend crop fruiting period, supplement elements, strengthen roots and seedlings, and slow down the occurrence of soil-borne diseases. Summary of the invention
[0006] The purpose of the present invention is to provide an application method of a suspension composition for preventing and controlling cucumber leafminers. The method can not only effectively prevent and control cucumber leafminers, but also protect new leaves from being harmed, resist abiotic stress of crops, timely and effectively alleviate the stress of drought, waterlogging, freezing and fertilizer damage, increase cucumber yield, and slow down the occurrence of soil-borne diseases.
[0007] The technical solution of the present invention is:
[0008] Application method of a suspension composition for controlling Liriomyza sativae in cucumbers, characterized in that: the application method is to perform foliar spraying of the suspension composition at the initial stage of the occurrence of Liriomyza sativae in cucumbers with an active ingredient dosage of 45 - 90 grams per hectare, with a water consumption of 600 - 900 kilograms per hectare, a spraying interval of 7 - 10 days each time, and a total of 2 sprays.
[0009] The suspension composition, based on 1000 parts by weight, comprises the following raw material components: 50 - 450 parts by weight of cyromazine, 10 - 150 parts by weight of cyantraniliprole, 20 - 300 parts by weight of a biological synergist, 50 - 400 parts by weight of an auxiliary agent, and the balance of water, wherein the weight ratio of cyromazine to cyantraniliprole is 7:1 - 3:1, and preferably the weight ratio of cyromazine to cyantraniliprole is 3:1.
[0010] Further, the weight ratio of cyromazine, cyantraniliprole, and the biological synergist is 15:5:(1 - 10), and the preferred weight ratio is 15:5:5.
[0011] Further, the biological synergist is a protein hydrolysate or a seaweed extract. The protein hydrolysate is a mixture of polypeptides, oligopeptides, and amino acids; the seaweed extract is selected from one or more of salts, polyamines, and polyphenols.
[0012] Further, the auxiliary agent is selected from one or more of a dispersant, a wetting agent, an antifreeze, a thickener, an antifoaming agent, and a preservative. The dispersant is selected from one or more of alkyl naphthalene sulfonates, alkyl naphthalene sulfonate formaldehyde polymers, lignin sulfonates, polycarboxylates, and EO - PO block copolymers; the wetting agent is selected from one or more of fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers (nonylphenol polyoxyethylene ether and octylphenol polyoxyethylene ether), fatty amine polyoxyethylene ethers, alkyl glycosides, trisiloxane polyoxyethylene ethers, polyaryl phenol polyoxyethylene ethers, and castor oil polyoxyethylene ethers; the antifreeze is selected from one or more of ethylene glycol, propylene glycol, and glycerol; the preservative is sodium benzoate; the antifoaming agent is a silicone antifoaming agent, a C10 - 20 saturated fatty acid compound, or a C8 - 10 fatty alcohol; the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, and carboxymethyl cellulose.
[0013] Further, based on 1000 parts by weight, the suspension composition consists of the following components in the following contents: 180 - 360 parts by weight of cyromazine, 60 - 120 parts by weight of cyantraniliprole, 20 - 240 parts by weight of a biological synergist, 15 - 75 parts by weight of a dispersant, 15 - 75 parts by weight of a wetting agent, 0 - 60 parts by weight of an antifreeze, 2 - 25 parts by weight of a thickener, 0.5 - 15 parts by weight of an antifoaming agent, 0 - 20 parts by weight of a preservative, and the balance of water.
[0014] Further, based on 1000 parts by weight, the suspension composition is composed of the following components: 300 parts by weight of cyromazine, 100 parts by weight of cyantraniliprole, 30 - 200 parts by weight of biological synergist, 20 - 60 parts by weight of dispersant, 15 - 50 parts by weight of wetting agent, 20 - 50 parts by weight of antifreeze, 5 - 15 parts by weight of thickener, 0.5 - 10 parts by weight of defoamer, 3 - 15 parts by weight of preservative, and the balance is water.
[0015] Compared with the prior art, the composition of the present invention has the following beneficial effects: (1) It can effectively control Liriomyza sativae in cucumbers, solve the problems of poor pesticide permeability and dispersibility, can quickly penetrate through the epidermal layer of crops, reach the damage site of Liriomyza sativae, and quickly kill the larvae of Liriomyza sativae through strong systemic conduction; (2) Protect the new leaves from damage, can also resist abiotic stresses of crops, timely and effectively relieve the stresses of drought, waterlogging, freezing and fertilizer damage, improve soil quality, purify the soil, extend the fruiting period of crops, supplement elements, strengthen roots and seedlings; (3) Slow down the occurrence of soil-borne diseases; (4) Environmentally friendly, safe, with low residues and a short pre-harvest interval. Detailed implementation manners
[0016] The following further illustrates the present invention in conjunction with embodiments, but the present invention is not limited thereto.
[0017] Application Example 1 The suspension composition disclosed in the following examples contains the following components based on 1000 parts by weight:
[0018] Example 1 400 g / L Cyromazine · Cyantraniliprole Suspension
[0019] 300 parts by weight of cyromazine, 100 parts by weight of cyantraniliprole, 50 parts by weight of keratinase, 25 parts by weight of lignosulfonate, 15 parts by weight of EO-PO block copolymer, 30 parts by weight of fatty alcohol polyoxyethylene ether, 30 parts by weight of ethylene glycol, 5 parts by weight of polyvinyl alcohol, 3 parts by weight of silicone defoamer, 5 parts by weight of sodium benzoate, and the balance is water, to obtain 400 g / L Cyromazine · Cyantraniliprole Suspension.
[0020] Example 2 400 g / L Cyromazine · Cyantraniliprole Suspension
[0021] 300 parts by weight of cyromazine, 100 parts by weight of cyantraniliprole, 100 parts by weight of lysine, 30 parts by weight of alkylnaphthalenesulfonate, 10 parts by weight of polycarboxylate, 20 parts by weight of alkylphenol polyoxyethylene ether, 15 parts by weight of alkyl glycoside, 50 parts by weight of propylene glycol, 6 parts by weight of xanthan gum, 2 parts by weight of C8-10 fatty alcohols, 3 parts by weight of sodium benzoate, and the balance is water, to obtain 400 g / L Cyromazine · Cyantraniliprole Suspension.
[0022] Example 3 120 g / L Cyromazine · Cyantraniliprole Suspension
[0023] 90 parts by weight of cyromazine, 30 parts by weight of cyantraniliprole, 80 parts by weight of glycine, 25 parts by weight of alkylnaphthalenesulfonate formaldehyde polymer, 20 parts by weight of castor oil polyoxyethylene ether, 40 parts by weight of propylene glycol, 10 parts by weight of carboxymethyl cellulose, 5 parts by weight of silicone defoamer, 4 parts by weight of sodium benzoate, with the balance being water, to prepare a 120 g / L cyromazine·cyantraniliprole suspension concentrate.
[0024] Example 4: 240 g / L cyromazine·cyantraniliprole suspension concentrate
[0025] 180 parts by weight of cyromazine, 60 parts by weight of cyantraniliprole, 60 parts by weight of erythritol, 28 parts by weight of alkylnaphthalenesulfonate, 20 parts by weight of alkyl polyglycoside, 10 parts by weight of trisiloxane polyoxyethylene ether, 30 parts by weight of propylene glycol, 5 parts by weight of polyvinyl alcohol, 1 part by weight of C8-10 fatty alcohols, 7 parts by weight of sodium benzoate, with the balance being water, to prepare a 240 g / L cyromazine·cyantraniliprole suspension concentrate.
[0026] Example 5: 480 g / L cyromazine·cyantraniliprole suspension concentrate
[0027] 360 parts by weight of cyromazine, 120 parts by weight of cyantraniliprole, 50 parts by weight of methionine, 10 parts by weight of polycarboxylate, 30 parts by weight of EO-PO block copolymer, 15 parts by weight of sodium dodecylbenzenesulfonate, 10 parts by weight of fatty alcohol polyoxyethylene ether, 50 parts by weight of ethylene glycol, 2 parts by weight of polyvinyl alcohol, 1.5 parts by weight of silicone defoamer, 6 parts by weight of sodium benzoate, with the balance being water, to prepare a 480 g / L cyromazine·cyantraniliprole suspension concentrate.
[0028] Example of Application 2:
[0029] Indoor joint toxicity determination experiment of cyromazine, cyantraniliprole and their mixtures against Liriomyza sativae
[0030] 1 Test purpose
[0031] To determine the toxicity of cyromazine and cyantraniliprole and their different mixing combinations against Liriomyza sativae indoors, evaluate the synergistic effect, clarify their compatibility, and provide a scientific basis for the research and development of the mixture of cyromazine and cyantraniliprole.
[0032] 2 Experimental conditions
[0033] 2.1 Test target
[0034] Liriomyza sativae Blanchard. The test targets used were healthy 2nd instar larvae collected from a cucumber field that had never been treated with pesticides in the test base for the co-determination experiment.
[0035] 2. Culture conditions
[0036] The culture conditions of the test targets and the targets after the test were a temperature of (25±1)°C, a relative humidity of 60% - 80%, and a light cycle of L∶D = (16∶8) h.
[0037] 3 Experimental design
[0038] 3.1 Test agents
[0039] Cyromazine 98% technical; Cyantraniliprole 94% technical.
[0040] 3.2 Agent preparation
[0041] Weigh 0.0102 g of cyromazine 98% technical, dissolve it in 2 mL of acetone, add 0.2 mL of Tween80 emulsifier, stir evenly, add water to 100 mL to prepare a stock solution of 100 mg / L. Then take 20 mL of the stock solution and add it to 80 mL of water containing 0.1% Tween80 emulsifier to prepare a test solution of 20 mg / L. Then dilute it with water containing 0.1% Tween80 emulsifier at a 2-fold ratio to 10, 5, 2.5, 1.25, and 0.625 mg / L, and conduct tests at a total of 6 concentrations.
[0042] Weigh 0.0106 g of cyantraniliprole 94% technical, dissolve it in 1 mL of acetone, add 0.2 mL of Tween80 emulsifier, stir evenly, add water to 100 mL to prepare a stock solution of 100 mg / L. Then take 8 mL of the stock solution and add it to 92 mL of water containing 0.1% Tween80 emulsifier to prepare a test solution of 8 mg / L. Then dilute it with water containing 0.1% Tween80 emulsifier at a 2-fold ratio to 4, 2, 1, 0.5, and 0.25 mg / L, and conduct tests at a total of 6 concentrations.
[0043] According to the ratios of cyromazine to cyantraniliprole of 7∶1, 5∶1, 3∶1, 1∶1, and 1∶3, respectively measure 7, 6.67, 6, 4, and 2 mL of the cyromazine stock solution, and then correspondingly measure 1, 1.33, 2, 4, and 6 mL of the cyantraniliprole stock solution, mix the two, and respectively make up to 100 mL with water containing emulsifier to prepare test solutions of 8 mg / L. Then dilute them with water containing 0.1% Tween80 emulsifier at a 2-fold ratio to 6 concentrations each for testing.
[0044] Add water containing 0.1% Tween80 emulsifier to 100 mL in a beaker containing 2 mL of acetone as the blank control.
[0045] 4 Test methods
[0046] Refer to the Guidelines for Pesticide Bioassays in the Laboratory NY / T 1154.14-2008, and the leaf-dipping method was used. More than 15 insects were placed in each treatment, with 4 replicates for each treatment. After the treatment, they were placed in an observation room for cultivation.
[0047] 5 Data Investigation and Statistical Analysis
[0048] 5.1 Investigation Time and Method
[0049] After 72 h, check the mortality of the test insects and record it. The criterion for judging the death of the test insects is that there is no response when gently touching the test insects with a pointed writing brush, which is counted as death.
[0050] 5.2 Data Statistical Analysis
[0051] Refer to the Guidelines for Pesticide Bioassays in the Laboratory NY / T 1154.7-2006, and the co-toxicity coefficient method of Sun & Johnson (1960) was used to calculate the co-toxicity coefficient (CTC) of each mixture combination. CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The calculation formula for the co-toxicity coefficient is as follows:
[0052]
[0053] The theoretical toxicity index (TTI) of the mixture = the toxicity index of pesticide A × the percentage (%) of pesticide A in the mixture + the toxicity index of pesticide B × the percentage (%) of pesticide B in the mixture
[0054]
[0055] 6 Experimental Results
[0056] The results of the toxicity determination of cyromazine, cyantraniliprole and their mixtures in the ratios of 7:1, 5:1, 3:1, 1:1 and 1:3 against Liriomyza sativae on cucumber are shown in Table 1. It can be seen from the table that the mixtures of cyromazine and cyantraniliprole in the above 5 ratios showed synergistic effects against Liriomyza sativae on cucumber except for 1:1 and 1:3. Their LC 50 values were 1.32, 1.17, 1.02, 1.07 and 0.88 mg / L respectively, and the co-toxicity coefficients were 132.79, 142.91, 150.09, 114.17 and 115.48 respectively. Among them, the synergistic effect of 3:1 was the most significant.
[0057] Table 1 Results of the toxicity determination of the mixture of cyromazine and cyantraniliprole against Liriomyza sativae on cucumber
[0058]
[0059] The results of this indoor bioassay showed that the mixing ratios of cyromazine and cyantraniliprole in the range of 7:1, 5:1 and 3:1 all showed synergistic effects on the cucumber leafminer, among which the synergistic effect of 3:1 was the most significant. Therefore, based on the comparison of synergistic toxicity alone, 3:1 can be regarded as the optimal mixing ratio of cyromazine and cyantraniliprole.
[0060] Application Example 3: Example Field Efficacy Test for Controlling Cucumber Liriomyza Sativus Blanchard 1 Test Purpose
[0061] To verify the efficacy of the 400 g / L cypermethrin-cyanamide suspension developed by the applicant on the control of cucumber leafminer and its safety to cucumbers, to clarify the field dosage and application technology, and to provide a scientific basis for pesticide registration.
[0062] 2 Test basis
[0063] Agricultural Industry Standard of the People's Republic of China "Quality Management Specification for Field Efficacy Tests for Pesticide Registration" (GB / T17980.66-2004), "GB / T 17980.66-2004 Pesticide Field Efficacy Test Guidelines (II) Part 66 Insecticides for Control of Vegetable Leaf Miners" and "SOP-I-006 Standard Operating Procedure for Field Efficacy Tests of Insecticides for Control of Vegetable Leaf Miners".
[0064] 3 Experimental location
[0065] This experiment was carried out in Sichuan and Shaanxi provinces.
[0066] 4. Selection of experimental subjects, crops and varieties
[0067] Test subjects: Liriomyza sativae leafminer;
[0068] Experimental crop: cucumber, variety Zhongnong No. 8.
[0069] 5 Experimental design and arrangement
[0070] 5.1 Dosage and number of medicines
[0071] Table 2 Experimental design of test drugs
[0072]
[0073]
[0074] 5.2 Application time and frequency
[0075] During the early larval stage of cucumber leafminer, spraying was used, and only one application was made during the entire experimental period.
[0076] 5.3 Usage capacity
[0077] 50 liters of spray liquid per mu, and 750 liters of spray liquid per hectare.
[0078] 5.4 Investigation time and frequency
[0079] Investigate before pesticide application, 3 days, 7 days and 10 days after pesticide application.
[0080] 5.5 Calculation method of pesticide efficacy
[0081]
[0082] 6 Experimental results
[0083] Table 3 Field efficacy test results of 400 g / L cyromazine·cyantraniliprole suspension concentrate against Liriomyza sativae Blanchard on cucumber
[0084]
[0085] The experimental results show that when 400 g / L cyromazine·cyantraniliprole suspension concentrate is used to control Liriomyza sativae Blanchard on cucumber, with the application rates of active ingredients being 84 g / ha and 66 g / ha respectively, and applied once during the early larval stage of Liriomyza sativae Blanchard, the control effects both reach over 82%, and the control effects are significantly better than those of the control single agents, i.e., 168.75 g / ha of 75% cyromazine wettable powder and 27 g / ha of 10% cyantraniliprole dispersible oil suspension concentrate. According to the variance analysis, the comparison of the control effects of several experimental treatments at 7 days and 10 days after application shows that the treatment of 400 g / L cyromazine·cyantraniliprole suspension concentrate is significantly different from the control single agent treatment at the 0.05 and 0.01 levels, and the effect is significantly better than the control single agent.
[0086] The above content is a further detailed description of the present invention in combination with specific preferred implementation manners, and it cannot be determined that the specific implementation of the present invention is only limited to these descriptions. For those of ordinary skill in the technical field to which the present invention belongs, without departing from the concept of the present invention, several simple deductions or substitutions can still be made, and all should be regarded as belonging to the protection scope of the present invention.
Claims
1. A method for applying a suspension composition for controlling Liriomyza sativae in cucumbers, characterized in that: The application method is to spray the suspension composition on the stems and leaves at the initial stage of the occurrence of Liriomyza sativae Blanchard with an active ingredient dosage of 45 - 90 g / ha, with a water consumption of 600 - 900 kg per hectare, a spraying interval of 7 - 10 days each time, and a total of 2 sprays. The suspension composition, based on 1000 parts by weight, comprises the following raw materials: 50 - 450 parts by weight of cyromazine, 10 - 150 parts by weight of cyantraniliprole, 20 - 300 parts by weight of biological synergist, 50 - 400 parts by weight of auxiliary agent, and the balance of water. The weight ratio of cyromazine to cyantraniliprole is 7:1 - 3:
1.
2. The application method according to claim 1, characterized in that: The weight ratio of cyromazine to cyantraniliprole is 3:
1.
3. The application method according to claim 1, characterized in that: The weight ratio of cyromazine, cyantraniliprole and biological synergist is 15:5:(1 - 10).
4. The application method according to claim 4, characterized in that: The biological synergist is a protein hydrolysate or a seaweed extract. The protein hydrolysate is a mixture of polypeptides, oligopeptides and amino acids. The seaweed extract is selected from one or more of salts, polyamines and polyphenols.
5. The application method according to claim 1, characterized in that: The auxiliary agent is selected from one or more of dispersants, wetting agents, antifreezing agents, thickeners, defoaming agents and preservatives.
6. The application method according to any one of claims 1 to 5, characterized in that: The suspension composition, based on 1000 parts by weight, consists of components with the following contents: 180 - 360 parts by weight of cyromazine, 60 - 120 parts by weight of cyantraniliprole, 20 - 240 parts by weight of biological synergist, 15 - 75 parts by weight of dispersant, 15 - 75 parts by weight of wetting agent, 0 - 60 parts by weight of antifreezing agent, 2 - 25 parts by weight of thickener, 0.5 - 15 parts by weight of defoaming agent, 0 - 20 parts by weight of preservative, and the balance of water.
7. The application method according to claim 6, characterized in that: The suspension composition, based on 1000 parts by weight, consists of components with the following contents: 300 parts by weight of cyromazine, 100 parts by weight of cyantraniliprole, 30 - 200 parts by weight of biological synergist, 20 - 60 parts by weight of dispersant, 15 - 50 parts by weight of wetting agent, 20 - 50 parts by weight of antifreezing agent, 5 - 15 parts by weight of thickener, 0.5 - 10 parts by weight of defoaming agent, 3 - 15 parts by weight of preservative, and the balance of water.
8. The application method according to claim 7, characterized in that: The dispersant is selected from one or more of alkylnaphthalene sulfonates, alkylnaphthalene sulfonate formaldehyde polymers, lignosulfonates, polycarboxylates, EO - PO block copolymers. The wetting agent is selected from one or more of fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers (nonylphenol polyoxyethylene ether and octylphenol polyoxyethylene ether), fatty amine polyoxyethylene ethers, alkyl glycosides, trisiloxane polyoxyethylene ethers, polyaryl phenolic polyoxyethylene ethers, castor oil polyoxyethylene ethers. The antifreezing agent is selected from one or more of ethylene glycol, propylene glycol and glycerol. The preservative is sodium benzoate. The defoaming agent is a silicone defoaming agent, C10 - 20 saturated fatty acid compounds, C8 - 10 fatty alcohols. The thickener is selected from one or more of xanthan gum, polyvinyl alcohol and carboxymethyl cellulose.