Hepatocyte sample collecting device

Abstract

The invention discloses a hepatocyte sample collection device, and relates to the technical field of medical collection, the hepatocyte sample collection device comprises a pricking needle, a biopsy cylinder, a protective pull wire and a collection mechanism; the pricking needle is used for puncturing a hepatic vein blood vessel, the biopsy cylinder enters the blood vessel through the pricking needle pipeline, a soft magnetic material and an ultrasonic endoscope are arranged in the front end of the biopsy cylinder, and the soft magnetic material is controlled by external electromagnetic navigation equipment to move in the blood vessel and finally reaches a to-be-sampled part. The ultrasonic endoscope is used for measuring the specific position of the biopsy tube in real time and transmitting image signals to an external display end, one end of the protective traction line is connected with the rear end of the biopsy tube, the other end of the protective traction line penetrates through the pricking needle and is manually controlled by medical staff, and the collecting mechanism is arranged in the biopsy tube.

Description

Technical Field

[0001] The present invention relates to the technical field of medical collection, and particularly relates to a hepatocyte sample collection device. Background Art

[0002] At present, with the development of society, the medical level has become more and more mature, and hepatocyte sampling surgery has now become a minimally invasive surgery.

[0003] During sampling, a catheter is usually inserted through jugular vein puncture and enters the hepatic vein through the inferior vena cava; under the guidance of X-ray / ultrasound, a special biopsy needle (such as Quick-Core) is used to puncture the liver tissue through the vein wall; a 1.5-2 cm tissue strip is obtained, and the whole process of intravascular operation avoids abdominal bleeding.

[0004] However, the obtained samples usually mix with the blood in the blood vessels and the tissue fluid around the liver, resulting in a lower purity of the samples, affecting the subsequent test results, and the obtained samples are columnar, and a cutting process is required for subsequent microscopic detection, which is rather troublesome.

[0005] Therefore, it is necessary to invent a hepatocyte sample collection device to solve the above problems. Summary of the Invention

[0006] Aiming at the above problems, the present invention provides a hepatocyte sample collection device to solve the problems of low purity and insufficient integrity of the obtained samples.

[0007] The technical solution adopted by the present invention is as follows: it includes a puncture needle, a biopsy tube, a protective traction wire and a collection mechanism; the puncture needle is used to pierce the hepatic vein blood vessel, the biopsy tube enters the blood vessel through the puncture needle tube, a soft magnetic material and an ultrasonic endoscope are arranged inside the front end of the biopsy tube, and the soft magnetic material is controlled by an external electromagnetic navigation device to move inside the blood vessel and finally reach the sampling site to be sampled. The ultrasonic endoscope is used to monitor the specific position of the biopsy tube in real time and transmit image signals to an external display terminal. One end of the protective traction wire is connected to the rear end of the biopsy tube, and the other end passes through the puncture needle and is manually controlled by medical staff. The collection mechanism is arranged inside the biopsy tube.

[0008] Further, as a preference, the collection mechanism includes a servo motor, a sleeve shaft, a clamping strip, a connecting column, a collection cylinder, a gear disk, a rotating shaft and a gear; the servo motor is fixedly installed at the center of the upper end inside the biopsy cylinder, the sleeve shaft is fixed at the output end of the servo motor, and a clamping groove is formed inwardly on the side wall of the sleeve shaft. The clamping strip is slidably arranged through the clamping groove, the connecting column is fixed at the lower end of the clamping strip, and the connecting column is coaxially arranged with the sleeve shaft. The inner wall of the biopsy cylinder is provided with threads, the collection cylinder is threadedly connected with the biopsy cylinder, and the connecting column rotatably penetrates through the center of the upper end of the collection cylinder. The gear disk is fixed at the lower end of the connecting column. Four sleeve holes are formed in the inner circumference of the side wall of the collection cylinder. Four rotating shafts are respectively rotatably arranged in the four sleeve holes, and an annular groove one is formed on the side wall of the upper end of the collection cylinder. The annular groove one is communicated with the four sleeve holes. Four gears are respectively fixed at the upper ends of the four rotating shafts, and the gear disk meshes with the four gears.

[0009] Further, as a preference, a liquid storage tank is formed inside the side wall of the biopsy cylinder. A magnetic ring one is hermetically and slidably arranged in the liquid storage tank. A magnetic ring two is fixed inside the side wall of the upper end of the collection cylinder. The magnetic ring one and the magnetic ring two are horizontally arranged, and the mutually approaching ends of the magnetic ring one and the magnetic ring two are opposite magnetic poles.

[0010] Further, as a preference, a communication groove is formed at the upper end of the liquid storage tank and is communicated with the outside of the biopsy cylinder. An introduction groove is formed at the lower end of the liquid storage tank and is communicated with the inside of the biopsy cylinder. A one-way pressure valve one is arranged in the introduction groove, and its one-way conduction direction is towards the inside of the biopsy cylinder.

[0011] Further, as a preference, a blocking disk is fixed on the inner wall of the upper end part of the collection cylinder. A through hole is formed in the blocking disk, and a lead-out pipe is fixed in the through hole. An arc groove is formed on the gear disk, and the upper end of the lead-out pipe sequentially penetrates through the arc groove and the upper wall of the collection cylinder in a sealed manner. A one-way pressure valve two is arranged in the lead-out pipe, and its one-way conduction direction is upwards. A liquid discharge hole is formed on the upper wall of the biopsy cylinder.

[0012] Further, as a preference, the collection mechanism further includes a rotary cutting knife and a circumferential cutting knife; the rotary cutting knife is fixed at the lower end of the collection cylinder. An annular groove two is formed on the inner wall of the lower end of the collection cylinder. The annular groove two is communicated with the lower ends of the four sleeve holes. Four circumferential cutting knives are respectively fixed on the side walls of the lower ends of the four rotating shafts, and the circumferential cutting knives are in movable contact with the inner wall of the annular groove two.

[0013] Advantages of the present invention: Through the operation of the collection mechanism, the sample can always be kept in a physiological saline environment during cutting and sampling, avoiding the mixing of blood or tissue fluid around the liver with the sample, improving the sample purity, and further improving the purity of the subsequent test results; during cutting and sampling, the rotary cutter and the circular cutter are alternately used to achieve multi-layer sampling. At the same time, the samples taken are all in the form of complete and neat circular flakes, and there is no need to divide the samples for subsequent direct microscopic detection.

[0014] In addition to the purposes, features and advantages described above, the present invention has other purposes, features and advantages. The present invention will be further described in detail below with reference to the drawings. Brief Description of the Drawings

[0015] The drawings forming a part of this application are used to provide a further understanding of the present invention. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention.

[0016] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a schematic diagram of the internal structure of the biopsy cylinder of the present invention; Figure 3 is a schematic diagram of a partial structure of the collection mechanism of the present invention; Figure 4 is an enlarged view of part A of the present invention; Figure 5 is a schematic diagram of the structure of the lower end of the collection cylinder of the present invention; Figure 6 is a state diagram of the circular cutter of the present invention.

[0017] Reference numerals: 1, puncture needle; 2, biopsy cylinder; 3, protective traction wire; 4, collection mechanism; 41, servo motor; 42, sleeve shaft; 43, clamping strip; 44, connecting column; 45, collection cylinder; 46, toothed disc; 47, rotating shaft; 48, gear; 451, first annular groove; 21, liquid storage tank; 22, first magnetic ring; 49, second magnetic ring; 23, communication groove; 24, introduction groove; 25, first one-way pressure valve; 50, blocking disc; 51, outlet pipe; 461, arc groove; 52, rotary cutter; 53, circular cutter; 452, second annular groove; 221, liquid discharge hole. Detailed Description of the Embodiments

[0018] In order to make the purposes, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0019] In the description of the present invention, it should be understood that the orientation or positional relationships indicated by the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0020] Reference Figures 1 to 6 , a hepatocyte sample collection device, comprising a stylet 1, a biopsy cylinder 2, a protective traction wire 3 and a collection mechanism 4; the stylet 1 is used to pierce the hepatic vein blood vessel, the biopsy cylinder 2 enters the blood vessel through the stylet 1 pipeline, a soft magnetic material and an ultrasonic endoscope are arranged inside the front end of the biopsy cylinder 2, and the soft magnetic material is controlled by an external electromagnetic navigation device to move inside the blood vessel and finally reach the sampling site to be sampled. The ultrasonic endoscope is used to monitor the specific position of the biopsy cylinder 2 in real time and transmit it to an external display terminal as an image signal. One end of the protective traction wire 3 is connected to the rear end of the biopsy cylinder 2, and the other end passes through the stylet 1 and is manually controlled by medical staff. The collection mechanism 4 is arranged inside the biopsy cylinder 2.

[0021] In the above, the front end of the biopsy cylinder 2 can be moved and attached to the liver surface by means of the soft magnetic material therein through an external electromagnetic navigation device, so as to facilitate the subsequent sealed sampling.

[0022] In addition, when the biopsy cylinder 2 adheres to the blood vessel wall, the medical staff pulls the protective traction wire 3 to separate the biopsy cylinder 2 from the blood vessel wall, so that the biopsy cylinder 2 can reach the target position smoothly.

[0023] As a preferred embodiment, the collection mechanism 4 includes a servo motor 41, a sleeve shaft 42, a clamping strip 43, a connecting column 44, a collection cylinder 45, a toothed disc 46, a rotating shaft 47 and a gear 48; the servo motor 41 is fixedly installed at the center of the upper end inside the biopsy cylinder 2, the sleeve shaft 42 is fixed to the output end of the servo motor 41, and a clamping groove is formed inwardly on the side wall of the sleeve shaft 42. The clamping strip 43 is slidably penetrated and arranged in the clamping groove, the connecting column 44 is fixed to the lower end of the clamping strip 43, and the connecting column 44 is coaxially arranged with the sleeve shaft 42. The inner wall of the biopsy cylinder 2 is provided with threads, the collection cylinder 45 is threadedly connected to the biopsy cylinder 2, and the connecting column 44 is rotatably penetrated and arranged at the center of the upper end of the collection cylinder 45. The toothed disc 46 is fixed to the lower end of the connecting column 44. Four sleeve holes are formed in the inner circumference of the side wall of the collection cylinder 45. Four rotating shafts 47 are respectively rotatably arranged in the four sleeve holes, and an annular groove 451 is formed on the side wall of the upper end of the collection cylinder 45. The annular groove 451 communicates with the four sleeve holes. Four gears 48 are respectively fixed to the upper ends of the four rotating shafts 47, and the toothed disc 46 meshes with the four gears 48.

[0024] It should be noted that when the servo motor 41 drives the sleeve shaft 42 to rotate, the inner wall of the card slot of the sleeve shaft 42 can drive the card strip 43 to rotate synchronously, thereby driving the connecting column 44 and the gear disk 46 to rotate synchronously. By meshing the gear disk 46 with the four gears 48, the four rotating shafts 47 are driven to rotate synchronously and in the same direction.

[0025] As a preferred embodiment, a liquid storage tank 21 is provided in the side wall of the biopsy cylinder 2. A first magnetic ring 22 is hermetically and slidably arranged in the liquid storage tank 21. A second magnetic ring 49 is fixed in the upper side wall of the collection cylinder 45. The first magnetic ring 22 and the second magnetic ring 49 are horizontally arranged, and the mutually approaching ends of the first magnetic ring 22 and the second magnetic ring 49 are opposite magnetic poles.

[0026] That is to say, when the second magnetic ring 49 spirally moves downward with the collection cylinder 45, the second magnetic ring 49 will drive the first magnetic ring 22 to move downward synchronously through the attraction force.

[0027] As a preferred embodiment, a communication groove 23 is provided at the upper end of the liquid storage tank 21, and the communication groove 23 communicates with the outside of the biopsy cylinder 2. An introduction groove 24 is provided at the lower end of the liquid storage tank 21. The introduction groove 24 communicates with the inside of the biopsy cylinder 2, and a one-way pressure valve 25 is arranged in the introduction groove 24, and its one-way conduction direction is towards the inside of the biopsy cylinder 2.

[0028] Among them, physiological saline is provided in the liquid storage tank 21 below the first magnetic ring 22. Specifically, when the first magnetic ring 22 moves downward, it can squeeze the physiological saline in the liquid storage tank 21 and enter the biopsy cylinder 2 from the lower introduction groove 24.

[0029] As a preferred embodiment, a blocking plate 50 is fixed on the inner wall of the upper end of the collection cylinder 45. A through hole is provided on the blocking plate 50, and a lead-out pipe 51 is fixed in the through hole. An arc-shaped groove 461 is provided on the gear disk 46, and the upper end of the lead-out pipe 51 sequentially penetrates through the arc-shaped groove 461 and the upper end wall of the collection cylinder 45 in a sealed manner. A second one-way pressure valve is arranged in the lead-out pipe 51, and its one-way conduction direction is upward. A liquid discharge hole 221 is provided on the upper end wall of the biopsy cylinder 2.

[0030] It should be noted that the liquid discharge hole 221 can communicate the space above the collection cylinder 45 with the outside of the biopsy cylinder 2, avoiding the influence of air pressure change in the space above the collection cylinder 45 on the up and down movement of the collection cylinder 45.

[0031] Specifically, when the collection cylinder 45 and the first magnetic ring 22 move downward, the tissue fluid below the blocking plate 50 will be discharged from the lead-out pipe 51 into the space above the collection cylinder 45. At the same time, the physiological saline will enter the biopsy cylinder 2. When the collection cylinder 45 and the first magnetic ring 22 move upward, the tissue fluid in the space above the collection cylinder 45 will be discharged through the liquid discharge hole 221.

[0032] As a preferred embodiment, the collection mechanism 4 further includes a rotary cutter 52 and a circumferential cutter 53; the rotary cutter 52 is fixed to the lower end of the collection cylinder 45, and an annular groove two 452 is formed on the inner wall of the lower end of the collection cylinder 45. The annular groove two 452 communicates with the lower ends of the four sleeve holes. There are four circumferential cutters 53, which are respectively fixed on the lower side walls of the four rotating shafts 47, and the circumferential cutters 53 are in movable contact with the inner wall of the annular groove two 452.

[0033] Among them, the rotary cutter 52 is used for longitudinally cutting the liver, and the circumferential cutter 53 is used for transversely cutting the liver.

[0034] It should be explained that when the gear disk 46 meshes and drives with the gear 48, the rotating shaft 47 will drive the circumferential cutter 53 to rotate, so as to achieve transverse cutting. When the back of the knife or the tip of the circumferential cutter 53 is limited by the inner wall of the annular groove two 452, the rotating shaft 47 is limited, so that the gear 48 cannot continue to rotate. Therefore, when the gear disk 46 continues to rotate, it will push the gear 48 to revolve synchronously with the gear disk 46 through the extrusion force between the teeth, thereby driving the collection cylinder 45 to rotate. Therefore, when the circumferential cutter 53 contacts the inner wall of the annular groove two 452, if the servo motor 41 continuously drives the gear disk 46 to rotate in the same direction, the collection cylinder 45 will move downward in a spiral manner, so that when the circumferential cutter 53 is in the annular groove two 452, the rotary cutter 52 performs longitudinal cutting to ensure that the cut sample is in the shape of a complete circular sheet.

[0035] Specifically, before sampling hepatocytes, the front end wall of the biopsy cylinder 2 is closely attached to the surface of the liver, and then the servo motor 41 is started and the sleeve shaft 42 is driven to rotate counterclockwise. The sleeve shaft 42 will drive the gear disk 46 to rotate counterclockwise through the clamping strip 43 and the connecting column 44. At first, the gear disk 46 first meshes with the gear 48 to drive the rotating shaft 47 to rotate clockwise by a certain angle. When the back of the knife of the circumferential cutter 53 rotates to the inner wall of the annular groove two 452, that is Figure 6 in state a in the figure, the rotation of the rotating shaft 47 is blocked, resulting in the gear 48 being unable to continue rotating. Subsequently, the gear disk 46 will push the gear 48 to revolve counterclockwise, so that the gear 48 will drive the collection cylinder 45 to rotate. Since the collection cylinder 45 is directly threadedly connected to the biopsy cylinder 2, the collection cylinder 45 will move downward in a spiral manner. The tissue fluid in the space below the plug disk 50 will be discharged from the outlet pipe 51. When the collection cylinder 45 moves downward, the magnetic ring two 49 inside it moves downward and will attract the magnetic ring one 22 to move downward synchronously through magnetic force. Therefore, the physiological saline in the liquid storage tank 21 will be squeezed into the space below the plug disk 50 by the magnetic ring one 22, so that the tissue fluid in the space below the plug disk 50 will be replaced by physiological saline. That is to say, before cutting and sampling, the surface of the liver to be sampled is in physiological saline, avoiding the mixing of tissue fluid and liver samples, thereby improving the sample purity; When performing the first layer of sampling, at first, the back of the knife of the circumferential cutter 53 still contacts the inner wall of the annular groove two 452, that is Figure 6In state a, subsequently, the servo motor 41 continues to drive the sleeve shaft 42 to rotate counterclockwise, and the collection cylinder 45 will continue to spiral downward counterclockwise. As a result, the rotary cutter 52 will longitudinally cut the liver. When the cutting depth of the rotary cutter 52 reaches the required depth of the first layer, adjust the servo motor 41 to rotate clockwise, driving the gear disk 46 to rotate clockwise. In the initial stage of the clockwise rotation of the gear disk 46, the gear disk 46 will engage with the gear 48 to drive the rotating shaft 47 to rotate counterclockwise by a certain angle. During this process, the four circumferential cutters 53 will perform a horizontal cut at the lower end of the first layer depth. And during the horizontal cutting process of the circumferential cutters 53, the collection cylinder 45 remains stationary, that is, during the horizontal cutting process, the longitudinal cutting process stops. When the tip parts of the four circumferential cutters 53 contact the second annular groove 452, that is Figure 6 In state b, a complete circular sheet-like sample of one layer will be obtained, which is convenient for subsequent direct microscopic detection. When the tip parts of the circumferential cutters 53 contact the second annular groove 452, immediately turn off the servo motor 41; When taking samples of the second layer, start the servo motor 41 and drive the sleeve shaft 42 to rotate counterclockwise. In the initial stage of the counterclockwise rotation of the gear disk 46, the gear disk 46 will engage with the gear 48 to drive the rotating shaft 47 to rotate clockwise by a certain angle. When the back of the circumferential cutter 53 contacts the inner wall of the second annular groove 452 again, that is Figure 6 In state a, the rotating shaft 47 is limited. Subsequently, the collection cylinder 45 will continue to spiral downward and drive the rotary cutter 52 to perform longitudinal cutting. When the cutting depth of the rotary cutter 52 reaches the required depth of the second layer, adjust the servo motor 41 to rotate clockwise, driving the gear disk 46 to rotate clockwise. In the initial stage of the clockwise rotation of the gear disk 46, it will engage with the gear 48 to drive the rotating shaft 47 to rotate counterclockwise by a certain angle. During this process, the four circumferential cutters 53 will perform a horizontal cut at the lower end of the second layer depth. When the tip parts of the four circumferential cutters 53 contact the second annular groove 452 again, that is Figure 6 In state b, a complete circular sheet-like sample of the second layer will be obtained below the circular sheet-like sample of the first layer. And when the tip parts of the circumferential cutters 53 contact the second annular groove 452, immediately turn off the servo motor 41. Subsequently, when taking samples of multiple layers, repeat the above process in a cycle; In summary, the alternating use of the rotary cutter 52 and the circumferential cutters 53 can take multiple-layer samples from the liver lesion, and the samples taken are all in the form of complete circular sheets, which is convenient for subsequent direct microscopic detection.

[0036] When the cutting of the last layer of samples is completed, the circumferential cutters 53 are in Figure 6 In state b, the servo motor 41 continues to drive clockwise, and the gear disk 46 will drive the collection cylinder 45 to spiral upward synchronously clockwise. During this process, the four circumferential cutters 53 are always at the lower end of the lowermost layer of samples. That is to say, when the collection cylinder 45 moves upward, the four circumferential cutters 53 can lift the multiple-layer samples, ensuring that the samples can be moved into the biopsy cylinder 2 synchronously with the collection cylinder 45, so that the multiple-layer samples can be neatly arranged and completely taken out; When the collection cylinder 45 moves upward to the initial position, the sampling process ends, and the soft magnetic material is controlled by an external electromagnetic navigation device to drive the biopsy cylinder 2 to return along the original path.

[0037] It should be explained that during the spiral upward movement of the collection cylinder 45, since the volume of the space below the blocking disc 50 gradually increases, a negative pressure is formed in this space. This negative pressure can cause the first magnetic ring 22 to move downward, so that the physiological saline in the liquid storage tank 21 will be sucked into the space below the collection cylinder 45 through the introduction groove 24. Therefore, when the sample enters the biopsy cylinder 2, the physiological saline will fill the entire area around the sample. On the one hand, the physiological saline covers the surface of the sample to prevent tissue dehydration and cracking. On the other hand, it avoids the mixing of blood or tissue fluid with the sample, improves the purity of the sample, and improves the accuracy of subsequent test results.

[0038] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A hepatocyte sample collection device, characterized in that: The invention comprises a puncture needle (1), a biopsy tube (2), a protective traction line (3) and a collection mechanism (4); the puncture needle (1) is used to puncture a hepatic vein, the biopsy tube (2) enters the blood vessel through a tube of the puncture needle (1), a soft magnetic material and an ultrasonic endoscope are arranged in the front end of the biopsy tube (2), and the soft magnetic material is controlled to move in the blood vessel by an external electromagnetic navigation device and finally reaches a part to be sampled, the ultrasonic endoscope is used to monitor the specific position of the biopsy tube (2) in real time and transmit the image signal to an external display end, one end of the protective traction line (3) is connected to the rear end of the biopsy tube (2), and the other end passes through the puncture needle (1) and is manually controlled by medical staff, and the collection mechanism (4) is arranged in the biopsy tube (2).

2. A liver cell sample collection device according to claim 1, characterized in that: The collecting mechanism (4) comprises a servo motor (41), a sleeve shaft (42), a clamping strip (43), a connecting column (44), a collecting tube (45), a toothed disc (46), a rotating shaft (47) and a gear (48); the servo motor (41) is fixedly mounted at the center of the upper end of the biopsy tube (2); the sleeve shaft (42) is fixed to the output end of the servo motor (41); a clamping slot is provided inwardly on the side wall of the sleeve shaft (42); the clamping strip (43) is slidably penetrated in the clamping slot; the connecting column (44) is fixed to the lower end of the clamping strip (43); the connecting column (44) and the sleeve shaft (42) are coaxially arranged; the inner wall of the biopsy tube (2) is provided with a threaded The collecting tube (45) is threadedly connected to the biopsy tube (2), and the connecting column (44) is rotatably arranged through the center of the upper end of the collecting tube (45). The toothed disc (46) is fixed to the lower end of the connecting column (44). Four sleeve holes are opened on the inner circumference of the side wall of the collecting tube (45). Four rotating shafts (47) are arranged and are rotatably arranged in the four sleeve holes respectively. An annular groove (451) is opened on the side wall of the upper end of the collecting tube (45), and the annular groove (451) is communicated with the four sleeve holes. Four gears (48) are arranged and are respectively fixed on the upper ends of the four rotating shafts (47), and the toothed disc (46) is meshed with the four gears (48).

3. A liver cell sample collection device according to claim 2, characterized in that: A liquid storage tank (21) is provided in the side wall of the biopsy tube (2), a magnetic ring 1 (22) is provided in a sealing and slidable manner in the liquid storage tank (21), a magnetic ring 2 (49) is fixed in the upper side wall of the collection tube (45), the magnetic ring 1 (22) and the magnetic ring 2 (49) are arranged horizontally, and the magnetic ring 1 (22) and the magnetic ring 2 (49) are close to each other at one end and have opposite magnetic poles.

4. A liver cell sample collection device according to claim 3, characterized in that: The upper end of the liquid storage tank (21) is provided with a communication groove (23), and the communication groove (23) is connected to the outside of the biopsy tube (2); the lower end of the liquid storage tank (21) is provided with an introduction groove (24), and the introduction groove (24) is connected to the inside of the biopsy tube (2); a one-way pressure valve (25) is arranged in the introduction groove (24), and its one-way guide direction is toward the inside of the biopsy tube (2).

5. A liver cell sample collection device according to claim 2, characterized in that: A blocking disk (50) is fixed on the inner wall of the upper end of the collecting tube (45), a through hole is formed on the blocking disk (50), a guide tube (51) is fixed in the through hole, an arc groove (461) is formed on the toothed disk (46), and the upper end of the guide tube (51) seals and penetrates the arc groove (461) and the upper end wall of the collecting tube (45) in sequence, a one-way pressure valve (2) is arranged in the guide tube (51), and the one-way guide direction thereof faces upwards, and a drainage hole (221) is formed on the upper end wall of the biopsy tube (2).

6. A liver cell sample collection device according to claim 2, characterized in that: The collecting mechanism (4) further comprises a rotary cutter (52) and a circular cutter (53); the rotary cutter (52) is fixed to the lower end of the collecting cylinder (45); a second circular groove (452) is provided on the inner wall of the lower end of the collecting cylinder (45); the second circular groove (452) is communicated with the lower ends of the four sleeve holes; four circular cutters (53) are provided and are respectively fixed to the lower end side walls of the four rotating shafts (47); and the circular cutters (53) are in active contact with the inner wall of the second circular groove (452).

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