A fullerene-containing composition with wrinkle-lightening and brightening effects, as well as its preparation method and application

Abstract

The present invention discloses a light wrinkle brightening composition containing fullerene and its preparation method and application, which specifically belong to the field of skin care products. The light wrinkle brightening composition containing fullerene includes the following components in parts by mass: 1-5 parts of water-soluble fullerene, 0.1-0.8 parts of 4-butylresorcinol and 4-10 parts of plant extract components, and the plant extract components are compounded by peony root extract, amur cork bark extract and white willow bark extract. The present invention uses water-soluble fullerene, 4-butylresorcinol, peony root extract, amur cork bark extract and white willow bark extract in combination, which can effectively inhibit elastase activity and eliminate DPPH free radicals. At the same time, these ingredients have an efficient promoting effect on the regeneration of skin collagen. The skin resident agent containing the light wrinkle brightening composition provided by the present invention has excellent efficacy performance in tightening skin, reducing wrinkles, and whitening and brightening skin color.

Description

Technical Field

[0001] The present invention belongs to the field of skin care products, and in particular relates to a wrinkle-lightening and brightening composition containing fullerene, and a preparation method and application thereof. Background Art

[0002] As the body's largest organ, the skin is in direct contact with the external environment, making it susceptible to external factors such as oxidation, dryness, and ultraviolet radiation. It is also affected by internal factors such as aging, dietary habits, and lifestyle. This can lead to a gradual decline in skin function, resulting in dryness, roughness, wrinkles, dullness, and sagging, which can seriously impact personal image and self-confidence. With improved living standards and increased awareness of personal image, consumers are increasingly demanding cosmetics that can effectively improve skin condition, especially products with wrinkle-lightening and brightening properties, which have become a hot demand in the market. However, most existing wrinkle-lightening products on the market suffer from insignificant results and limited mechanisms of action, making them unable to meet consumers' expectations for effective and comprehensive skin care. Summary of the Invention

[0003] In view of the shortcomings of the existing technology, the purpose of the present invention is to provide a wrinkle-lightening and brightening composition containing fullerene, which improves skin condition from multiple angles through the synergistic effect of multiple active ingredients and meets consumers' demand for high-quality cosmetics.

[0004] In order to achieve the above object, the present invention discloses the following technical solutions:

[0005] In a first aspect, the present invention provides a wrinkle-lightening and brightening composition containing fullerene, wherein the composition comprises the following components in parts by mass:

[0006] 1-5 parts of water-soluble fullerene;

[0007] 0.1-0.8 parts of 4-butylresorcinol;

[0008] 4-10 parts of plant extract components;

[0009] The plant extract component is prepared by compounding peony root extract, phellodendron amurense bark extract and willow bark extract.

[0010] Water-soluble fullerene, 4-butylresorcinol, peony root extract, amur cork bark extract and white willow bark extract each have unique benefits in the formula, and when used together, they have a significant effect on reducing wrinkles and brightening.

[0011] Water-soluble fullerene has strong antioxidant capacity, can scavenge free radicals, reduce skin oxidative damage, prevent wrinkles and brighten skin tone, while promoting collagen production and enhancing skin elasticity;

[0012] 4-Butylresorcinol inhibits the activity of tyrosinase and TRP-1, blocks melanin production, reduces pigment deposition, and achieves whitening and spot-lightening effects;

[0013] Peony root extract is rich in a variety of active ingredients and has antioxidant, anti-inflammatory, moisturizing and pigmentation inhibition effects. It can improve uneven skin tone, make the skin brighter and more even, while promoting collagen production and enhancing skin firmness.

[0014] Phellodendron amurense bark extract has antibacterial, anti-inflammatory and antioxidant effects, which can soothe the skin, reduce inflammation, reduce pigmentation and maintain skin health;

[0015] White Willow Bark Extract has moisturizing and antioxidant properties that can eliminate free radicals and protect the skin, while promoting collagen production and improving skin firmness.

[0016] The present invention has found that these ingredients work synergistically with each other in terms of wrinkle lightening and brightening. Water-soluble fullerene, peony root extract, phellodendron chinense bark extract and white willow bark extract jointly act as antioxidants and enhance the skin's ability to resist free radicals; 4-butylresorcinol and peony root extract work together to inhibit melanin production and brighten the skin tone; phellodendron chinense bark extract, peony root extract and white willow bark extract jointly exert anti-inflammatory and soothing effects, reduce pigmentation, promote collagen production, and inhibit elastase activity; Taken together, these ingredients improve skin condition from multiple angles, reduce wrinkles, brighten skin tone, inhibit pigmentation, relieve inflammation, and achieve a comprehensive wrinkle lightening and brightening effect.

[0017] Preferably, the composition comprises the following components in parts by mass:

[0018] 2-4 parts of water-soluble fullerene;

[0019] 0.1-0.3 parts of 4-butylresorcinol;

[0020] 5-7 parts of plant extract components;

[0021] The plant extract component is prepared by compounding peony root extract, phellodendron amurense bark extract and willow bark extract in a mass ratio of 1:(3-6):(1-2).

[0022] Further preferably, the composition comprises the following components in parts by mass:

[0023] 3 parts of water-soluble fullerene;

[0024] 0.2 parts of 4-butylresorcinol;

[0025] 6 parts of plant extract components.

[0026] In a second aspect, the present invention provides a skin retention agent with wrinkle-lightening and brightening effects, comprising the wrinkle-lightening and brightening composition containing fullerene described in the first aspect.

[0027] Preferably, the amount of the fullerene-containing wrinkle-lightening composition added to the skin retention agent is 1-3 wt %.

[0028] More preferably, the skin leave-on agent further comprises base oil, emulsifier, preservative, moisturizer, pH regulator and solvent.

[0029] More preferably, the base oil is at least one of olive oil and jojoba seed oil;

[0030] The emulsifier is at least one of laureth-23, polysorbate 80, and hydroxyethyl cellulose;

[0031] The moisturizing agent is at least one of glycerin, sodium hyaluronate, dipropylene glycol, and 1,2-pentanediol;

[0032] The preservative is at least one of p-hydroxyacetophenone and phenoxyethanol;

[0033] The pH regulator is at least one of arginine, potassium hydroxide, citric acid, and sodium citrate;

[0034] The solvent is deionized water or pure water.

[0035] In a third aspect, the present invention provides a method for preparing the skin-retention agent with wrinkle-lightening and brightening effects described in the second aspect, comprising the following steps:

[0036] Step 1. Stir and mix the base oil and emulsifier to obtain solution A;

[0037] Step 2. 4-butylresorcinol, preservative, 1,2-pentanediol, glycerol, and dipropylene glycol were stirred and mixed to obtain solution B;

[0038] Step 3. Stir and mix the water-soluble fullerene, plant extract, sodium hyaluronate, and deionized water to obtain solution C;

[0039] Step 4. Mix solution A, solution B, and solution C, perform high-speed homogenization and dispersion using a homogenizer, add a pH adjuster to adjust the pH value to 6.5-7, and obtain a skin resident agent.

[0040] In a fourth aspect, the present invention provides the use of the fullerene-containing wrinkle-lightening and brightening composition described in the first aspect in the preparation of a skin care product with wrinkle-lightening, firming, anti-wrinkle, anti-aging, whitening and / or spot-lightening effects.

[0041] Beneficial effects of the present invention:

[0042] 1. The present invention uses water-soluble fullerene, 4-butylresorcinol, peony root extract, amur cork bark extract and white willow bark extract in combination to effectively inhibit elastase activity, scavenge DPPH free radicals, and effectively promote the regeneration of collagen in the skin;

[0043] 2. The skin retention agent provided by the present invention, which contains the wrinkle-lightening and brightening composition, has excellent efficacy in tightening the skin, reducing wrinkles, and whitening and brightening the skin tone. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to the accompanying drawings.

[0045] Figure 1 This is a chart showing the changes in crow's feet after subject No. 20 used the product for 28 days.

[0046] Specific implementation method

[0047] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0048] Unless otherwise specified, the test methods used in the Examples and Comparative Examples are conventional methods; the materials, reagents, etc. used are commercially available unless otherwise specified; the percentages mentioned in the Examples and Comparative Examples are by mass unless otherwise specified.

[0049] In the present invention:

[0050] Water-soluble fullerene: purchased from Guangzhou Baihaobo Co., Ltd.

[0051] 4-Butylresorcinol: purchased from Zhuanglai Holding Group Co., Ltd.

[0052] Peony root extract: purchased from Shanghai Ruidian Biotechnology Co., Ltd.

[0053] Phellodendron amurense bark extract: purchased from Guangzhou Jizhou Trading Co., Ltd.

[0054] White willow bark extract: purchased from Guangzhou Jizhou Trading Co., Ltd.

[0055] Preparation of plant extract components:

[0056] Each raw material is accurately weighed according to the mass ratio of each raw material in Table 1, and each raw material is placed in a high-speed blender and stirred evenly to obtain the plant extract component.

[0057] Table 1 Raw materials and their mass ratios in plant extract components

[0058] raw material Component ① Component ② Component ③ Component ④ Component ⑤ Component ⑥ Component ⑦ Component ⑧ Peony Root Extract 1 1 1 - 1 1 1 4.5 Phellodendron amurense bark extract 3 4.5 6 4.5 - 4.5 1.5 1 White Willow Bark Extract 1 1.5 2 1.5 1.5 - 4.5 1.5

[0059] Note: “-” in the table means no additive.

[0060] Preparation of skin leave-on agent

[0061] Accurately weigh each component according to the dosage ratio of each component in Tables 2, 3, and 4;

[0062] Step 1. At 90°C, stir and mix the base oils at 500 rpm. After cooling to 50°C, add the emulsifier and stir and mix at 200 rpm to obtain solution A.

[0063] Step 2. 4-butylresorcinol, preservative, 1,2-pentanediol, glycerol, and dipropylene glycol were stirred and mixed at 30° C. and 500 rpm to obtain solution B;

[0064] Step 3. The water-soluble fullerene, plant extract, sodium hyaluronate and deionized water were stirred and mixed at 30° C. and 500 rpm to obtain solution C;

[0065] Step 4. Solution A, solution B, and solution C were mixed, and the mixture was homogenized and dispersed at high speed using a homogenizer for 20 minutes. A pH adjuster was added to adjust the pH value to 7 to obtain a skin leave-on agent.

[0066] Table 2 Components and mass ratios of the wrinkle lightening and brightening composition

[0067]

[0068] Table 3 Components and mass percentages of skin leave-on agents

[0069]

[0070]

[0071] Table 4 Comparison of the components and mass percentages of the skin leave-on agent

[0072]

[0073] Note: “-” in the table means no additive.

[0074] Performance Testing

[0075] 1 In vitro test

[0076] 1.1 Elastase inhibition test

[0077] Sample group: 10 μL of the skin resident agent prepared in Examples 1-5 and Comparative Examples 1-7 were added to a 96-well plate, and then 20 μL of a 0.1 U / mL elastase (porcine pancreas) solution was added. After incubation in a 25°C constant temperature incubator for 15 minutes, 50 μL of a 1 mg / mL substrate (98% purity N-succinyl-L-alanyl-L-alanine) was added. Finally, the absorbance value of the solution at 410 nm was measured using a microplate reader. X Three replicate wells were set up and the results were averaged.

[0078] Control group: 10 μL of deionized water was added to a 96-well plate, followed by 20 μL of 0.1 U / mL elastase (porcine pancreas) solution. The plate was incubated in a 25°C constant temperature incubator for 15 minutes, followed by the addition of 50 μL of 1 mg / mL substrate (98% purity N-succinyl-L-alanyl-L-alanine). The absorbance of the solution at 410 nm was measured using a microplate reader. O Three replicate wells were set up and the results were averaged.

[0079] The results were obtained according to the following elastase inhibition rate calculation formula, and the results are shown in Table 5.

[0080] Inhibition rate (%) = [(A O -A X ) / A O ]×100%

[0081] Table 5 Results of porcine pancreatic elastase inhibition rate

[0082] Group Elastase inhibition rate / % Example 1 73.12 Example 2 75.68 Example 3 79.13 Example 4 77.35 Example 5 74.08 Comparative Example 1 58.64 Comparative Example 2 61.42 Comparative Example 3 57.86 Comparative Example 4 64.47 Comparative Example 5 67.93 Comparative Example 6 42.61 Comparative Example 7 39.53

[0083] 1.2DPPH-free radical scavenging test

[0084] According to the method specified in T / SHRH 006-2018, the DPPH radical scavenging rate (%) of the skin resident agents prepared in Examples 1-5 and Comparative Examples 1-7 was respectively tested. The higher the DPPH radical scavenging rate, the better the anti-wrinkle effect of the skin resident agent. The results are shown in Table 6.

[0085] Table 6 DPPH-free radical scavenging rate

[0086]

[0087]

[0088] 1.3 Type I collagen synthesis promotion test

[0089] This experiment uses fibroblasts as the test object, establishes a cell photoaging damage model through UVA irradiation, and detects the changes in type I collagen (Collagen I) content after the test sample is exposed to the sun to evaluate the firming and anti-wrinkle efficacy of the test sample.

[0090] (1) Inoculation: 1×10 5 Cells were seeded into 96-well plates at a seeding density of 100 cells / well and placed in an incubator for overnight incubation (37° C., 5 v / v% CO 2 ).

[0091] (2) UVA irradiation: After washing the cells with PBS, the cells were divided into groups according to Table 7. The UVA irradiation group was irradiated with 30 J / cm 2 of UVA radiation.

[0092] (3) Solution Preparation: The skin resident agents prepared in Examples 1-5 and Comparative Examples 1-7 were used as the test sample solutions, and TGF-β1 (100 ng / mL) was used as the positive control. 100 μL of sample was added to each well, with three replicates per group. After administration, the 96-well plate was placed in a 37°C incubator in the dark and incubated for 24 h.

[0093] Table 7 Test groups

[0094]

[0095] (4) Collection of cell supernatant: 24 h after administration, the 96-well plate was removed and the cell supernatant corresponding to each sample group was collected into a centrifuge tube. The cells were centrifuged at 1000 rpm for 10 min. The supernatant was collected and placed into a 1.5 mL centrifuge tube and stored at -20°C for later use.

[0096] (5) Determination of type I collagen concentration in cell supernatant using ELISA kit: Remove the kit and the sample to be tested 30 minutes before the test and place them at room temperature before use. The test operation must be performed strictly in accordance with the kit instructions.

[0097] Table 8 Type I collagen content detection results

[0098] Sample name Type I collagen content (ng / mL) Type I collagen growth rate (%) Model comparison 1.329 / Blank (not damaged by UVA) 1.671 25.73 Positive (TGFβ 1100 ng / mL) 1.595 20.02 Example 1 1.801 35.52 Example 2 1.833 37.92 Example 3 1.874 41.01 Example 4 1.859 39.88 Example 5 1.822 37.10 Comparative Example 1 1.608 20.99 Comparative Example 2 1.591 19.71 Comparative Example 3 1.625 22.27 Comparative Example 4 1.773 33.41 Comparative Example 5 1.745 31.30 Comparative Example 6 1.483 11.59 Comparative Example 7 1.511 13.69

[0099] Result analysis:

[0100] According to the results in Tables 5-8, the skin resident agent provided by the present invention has excellent firming and anti-wrinkle effects. The wrinkle-lightening composition used in Comparative Examples 1-7 is based on the formula of the spot-lightening and brightening composition used in Example 3, except that the key ingredients are omitted and the mass ratios between the key ingredients are adjusted. The wrinkle-lightening composition in Comparative Example 1 lacks peony root extract, resulting in a significant decrease in its elastase inhibition performance, free radical scavenging performance and collagen-promoting performance. The wrinkle-lightening compositions in Comparative Examples 2 and 3 lack Phellodendron chinense bark extract and Salix alba bark extract, respectively, and also present similar results to Comparative Example 1, demonstrating the key role of peony root extract, Phellodendron chinense bark extract and Salix alba bark extract in the formula. The mass ratio of peony root extract, amur cork bark extract, and willow bark extract in the wrinkle-lightening compositions used in Comparative Examples 4-5 was outside the mass ratio range defined by the present invention. Their elastase inhibition, free radical scavenging, and collagen-promoting properties were significantly reduced compared to those in Example 3, demonstrating the key role of the specific dosage ratio of peony root extract, amur cork bark extract, and willow bark extract in the overall formulation. The spot-lightening compositions used in Comparative Examples 6-7 lacked water-soluble fullerenes and plant extracts, respectively, resulting in significant reductions in their elastase inhibition, free radical scavenging, and collagen-promoting properties. This demonstrates that water-soluble fullerenes, 4-butylresorcinol, and plant extracts also play an indispensable and key role in the formulation.

[0101] 2 Human trials

[0102] Wrinkle reduction test

[0103] 60 volunteers were selected as test subjects, all of whom had obvious crow's feet. The test subjects were first numbered 1-60 and then randomly divided into 6 groups, each with 10 people. Among them, the skin resident agents of Examples 1-5 were applied to the faces of 5 groups of test subjects respectively, and the skin resident agent of the blank control group was applied to the face of the test subject. The skin resident agent of the blank control group was based on the formula of Example 3 but lacked the effective components (i.e., lacked water-soluble fullerene, 4-butylresorcinol and plant extract components). The missing amount was supplemented with deionized water. The test subjects were applied once a day in the morning and evening, 2g each time, for a total of 28 days. The trial effect was tested using a skin tester. The results are shown in Table 9 and Figure 1 ,in Figure 1 This is a before-and-after comparison chart showing that the subject (No. 20) had improved significantly after using the skin leave-on agent prepared in Example 3 for 28 days.

[0104] Test indicators: Level ③: obvious crow's feet; Level ②: shallow crow's feet; Level ①: no crow's feet;

[0105] Test conditions: (1) Indoor environment; (2) No strong sunlight or direct light; (3) Ambient temperature 25±2℃, ambient humidity 40-60%.

[0106] Table 9 Crow's feet test results

[0107]

[0108] Brightening effect test

[0109] 60 female volunteers aged 18-30 were selected as subjects. The subjects were required to have healthy facial skin without obvious redness, skin lesions, scars, etc. The subjects were first numbered 1-60 and then randomly divided into 6 groups, 10 people in each group. Among them, the faces of 5 groups of subjects were respectively applied with the skin resident agents of Examples 1-5, and the face of 1 group of subjects was applied with the skin resident agent of the blank control group. The skin resident agent of the blank control group was based on the formula of Example 3 but lacked the effective components (i.e., lacked water-soluble fullerene, 4-butylresorcinol and plant extract components). The missing amount was supplemented with deionized water. It was applied once a day in the morning and evening, 2 g each time, for a total of 28 days. Before applying the skin resident agent, the initial L* value and ITA° value of the facial skin were collected using a skin test probe. The L* value and ITA° value of the facial skin were tested after 28 days of use. The L* value change rate and ITA° value change rate were calculated using the formula: change rate / % = (before use - after use) / before use × 100%. The average value of each group of results was taken. The results are shown in Table 10.

[0110] Table 10 Skin brightness data

[0111] Group L* value change rate / % ITA° value change rate / % Blank control group 2.16 0.16 Example 1 10.61 2.96 Example 2 12.47 3.24 Example 3 15.26 3.98 Example 4 13.08 3.31 Example 5 12.35 3.18

[0112] Result analysis:

[0113] According to Table 9, Table 10 and Figure 1 It can be seen that the skin resident agent provided by the present invention has excellent efficacy in reducing wrinkles and brightening. By comparison with the blank control group, it can be seen that the water-soluble fullerene, 4-butylresorcinol and plant extract components provided by the present invention play a key role in the overall formula. Among them, the wrinkle-reducing and brightening performance of Example 3 shows a better effect.

[0114] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. A wrinkle-lightening and brightening composition containing fullerene, characterized in that: The composition comprises the following components in parts by mass: 1-5 parts of water-soluble fullerene; 0.1-0.8 parts of 4-butylresorcinol; 4-10 parts of plant extract components; The plant extract component is prepared by compounding peony root extract, phellodendron amurense bark extract and willow bark extract.

2. The wrinkle-lightening and brightening composition containing fullerene according to claim 1, characterized in that: The composition comprises the following components in parts by mass: 2-4 parts of water-soluble fullerene; 0.1-0.3 parts of 4-butylresorcinol; 5-7 parts of plant extract components; The plant extract component is prepared by compounding peony root extract, phellodendron amurense bark extract and willow bark extract in a mass ratio of 1:(3-6):(1-2).

3. The wrinkle-lightening and brightening composition containing fullerene according to claim 2, characterized in that: The composition comprises the following components in parts by mass: 3 parts of water-soluble fullerene; 0.2 parts of 4-butylresorcinol; 6 parts of plant extracts.

4. A skin retaining agent with wrinkle-lightening and brightening effects, characterized in that: A wrinkle-lightening and brightening composition comprising the fullerene-containing composition according to any one of claims 1 to 3.

5. The skin retaining agent with wrinkle-lightening and brightening effects according to claim 4, characterized in that: The addition amount of the fullerene-containing wrinkle-lightening composition is 1-3 wt %.

6. The skin retaining agent with wrinkle-lightening and brightening effects according to claim 5, characterized in that: The skin retention agent further comprises base oil, emulsifier, preservative, moisturizer, pH regulator and solvent.

7. The skin retaining agent with wrinkle-lightening and brightening effects according to claim 6, characterized in that: The base oil is at least one of olive oil and jojoba seed oil; The emulsifier is at least one of laureth-23, polysorbate 80, and hydroxyethyl cellulose; The moisturizing agent is at least one of glycerin, sodium hyaluronate, dipropylene glycol, and 1,2-pentanediol; The preservative is at least one of p-hydroxyacetophenone and phenoxyethanol; The pH regulator is at least one of arginine, potassium hydroxide, citric acid, and sodium citrate; The solvent is deionized water or pure water.

8. The method for preparing the skin-retention agent with wrinkle-lightening and brightening effects according to claim 7, characterized in that: The following steps are involved: Step 1. Stir and mix the base oil and emulsifier to obtain solution A; Step 2. 4-butylresorcinol, preservative, 1,2-pentanediol, glycerol, and dipropylene glycol were stirred and mixed to obtain solution B; Step 3. Stir and mix the water-soluble fullerene, plant extract, sodium hyaluronate, and deionized water to obtain solution C; Step 4. Mix solution A, solution B, and solution C, perform high-speed homogenization and dispersion using a homogenizer, add a pH adjuster to adjust the pH value to 6.5-7, and obtain a skin resident agent.

9. Use of the fullerene-containing wrinkle-lightening and brightening composition according to any one of claims 1 to 3 in the preparation of a skin care product with wrinkle-lightening, firming, anti-wrinkle, whitening and / or spot-lightening effects.

10. Use of the wrinkle-lightening and brightening composition containing fullerene according to any one of claims 1 to 3 in preparing a skin care product with anti-aging efficacy.

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