A hair pore cleaning composition containing phellinus baumii and a preparation method and application thereof

Abstract

The application discloses a pore cleaning composition containing phellinus baumii, and a preparation method and application thereof, and belongs to the technical field of cosmetics. The pore cleaning composition provided by the application comprises the following components in mass fraction: bidirectional fermentation product 0.1-10 parts, guaiacum extract 0.1-5 parts, hydroxyl asiaticoside 0.01-1 part, coffee arabica seed extract 0.01-3 parts, and sophoramine 0.01-2 parts. The bidirectional fermentation product is a bidirectional fermentation product of phellinus baumii and lactobacillus plantarum. The application can inhibit sebum oxidation, prevent keratin accumulation, resist inflammation, kill bacteria and the like through the cooperation of the components in the application, so that the blackheads and whiteheads can be reduced quickly and long-acting, and the effect of shrinking pores can be achieved. Meanwhile, the pore cleaning composition provided by the application is mild and non-irritating, and is especially suitable for people with sensitive skin.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a pore cleaning composition containing Phellinus baumii and a preparation method and application thereof. BACKGROUND

[0002] The formation of blackheads and whiteheads is closely related to excessive sebum secretion, abnormal keratinization of hair follicles, and microbial proliferation. The excessive secretion of sebum by sebaceous glands will accumulate in the hair follicle and combine with the excessive proliferation of keratinocytes to form a keratin plug. The main difference between blackheads (open comedones) and whiteheads (closed comedones) lies in the exposure of the keratin plug, blackheads are due to the oxidation of sebum exposed to the air and turned black, while whiteheads are caused by the blockage of the hair follicle outlet leading to the accumulation of oil. The overgrowth of Propionibacterium acnes also promotes the formation of blackheads and causes skin inflammation. In addition, the long-term accumulation of sebum will expand the hair follicle, causing the pores to become large. Furthermore, repeated inflammatory reactions will further damage the tissue structure around the hair follicle, leading to the expansion of the pores.

[0003] The existing methods for removing blackheads and whiteheads are diverse, but each has its own limitations. Chemical exfoliation (such as salicylic acid, fruit acid, etc.) can effectively unclog pores, but it has strong irritation to the skin and long-term use may lead to damage to the skin barrier. Physical cleaning methods, such as adsorptive mud masks and comedone needles, can clean the pores in the short term, but the effect is not long-lasting and can easily cause skin damage, even exacerbate inflammation. Retinol and plant retinoids, which are keratin metabolism regulating ingredients, can improve the normal metabolism of the stratum corneum, and long-term use can help reduce the formation of keratin plugs, but the effect is slow to appear and has certain irritation, which may cause discomfort on sensitive skin. Therefore, the existing technology still has certain deficiencies in comprehensiveness, long-term effect, rapidity, and safety. SUMMARY

[0004] The present application aims to overcome the deficiencies of the prior art and provide a pore cleaning composition that can inhibit sebum oxidation, prevent keratin accumulation, and has multiple pathways such as anti-inflammatory and bactericidal effects, thereby effectively, quickly, gently, and long-term reducing blackheads and whiteheads and shrinking pores, as well as a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, in the first aspect of the present application, the present application provides a pore cleaning composition containing Phellinus baumii, which comprises the following components by mass:

[0006] 0.1-10 parts of bidirectional fermentation product, 0.1-5 parts of guaiac extract, 0.01-1 part of hydroxyasiaticoside, 0.01-3 parts of coffea arabica seed extract, and 0.01-2 parts of matrine.

[0007] The present application can achieve the effect of reducing blackheads and whiteheads quickly and long-term by selecting appropriate quality parts of components, interaction between components, and cooperation, thereby realizing the effect of reducing blackheads and whiteheads quickly and long-term, and shrinking pores; meanwhile, the pore cleaning composition provided by the present application is mild and non-irritating, and is particularly suitable for people with sensitive skin.

[0008] Specifically, the inventors speculate that each component has the following effects: the raw material of the bidirectional fermentation product, which is rich in triterpenoids and polysaccharides in Phellinus baumii, can effectively regulate sebum secretion, inhibit 5α-reductase activity, reduce excess oil, and prevent sebum oxidation through antioxidant effect to reduce blackhead formation; in addition, the bidirectional fermentation of Phellinus baumii and Lactobacillus plantarum can produce active substances that Phellinus baumii does not have before fermentation, thereby better achieving the effect of the present application. The guaiacwood extract contains guaiac resin acid and has significant anti-inflammatory soothing effect, which can quickly relieve inflammation around the hair follicle and effectively inhibit the growth of Propionibacterium acnes to reduce the infection of keratotic plugs caused by bacteria. The madecassic acid activates the PPAR-α receptor, promotes the synthesis of ceramides, thereby repairing the damaged skin barrier, and inhibits the NF-κB pathway to reduce skin inflammatory response and prevent inflammatory pore problems. The coffee arabica seed extract is rich in caffeine and chlorogenic acid, which can scavenge free radicals, promote microcirculation, help metabolic waste discharge, promote keratinocyte turnover, and assist in softening the keratotic plugs of hair follicles to reduce whitehead formation. Matrine regulates the TLR4 / MyD88 signaling pathway to inhibit the inflammatory response caused by Propionibacterium acnes and balance the skin immune response, thereby effectively reducing sensitive pore problems caused by immune hyperactivity. On the basis of the above-mentioned effects of the components, there are still unpredictable synergies or antagonisms between the components, but on the basis of the component combination and the quality parts of the components provided by the present application, the effect of the present application can be achieved.

[0009] As a preferred embodiment of the pore cleaning composition of the present application, the pore cleaning composition comprises the following components in the following quality parts: bidirectional fermentation product 0.5-5 parts, guaiacwood extract 0.5-3 parts, madecassic acid 0.05-0.5 parts, coffee arabica seed extract 0.1-1 parts, and matrine 0.05-0.5 parts.

[0010] As a preferred embodiment of the pore cleaning composition of the present application, the pore cleaning composition comprises the following components in the following quality parts: bidirectional fermentation product 1-2 parts, guaiacwood extract 1-2 parts, madecassic acid 0.1-0.2 parts, coffee arabica seed extract 0.3-0.5 parts, and matrine 0.2-0.3 parts.

[0011] The present application researches and finds that when the mass fraction of the components in the pore cleaning composition is further selected within the above range, the comprehensive performance of the obtained product is better.

[0012] As a preferred embodiment of the pore cleaning composition of the present application, the sum of the mass percentages of hydroxyl asiatic acid and matrine is 1-34% based on the total mass of the pore cleaning composition.

[0013] Exemplarily, the sum of the mass percentages of hydroxyl asiatic acid and matrine can be any point value or any two-point range value between 1-34% based on the total mass of the pore cleaning composition, such as 1%, 3%, 5%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, 30%, 34%, etc.

[0014] Preferably, the sum of the mass percentages of hydroxyl asiatic acid and matrine is 7-14% based on the total mass of the pore cleaning composition. For example, it can be 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, etc.

[0015] The present application researches and finds that the sum of the mass percentages of hydroxyl asiatic acid and matrine in the pore cleaning composition will affect the anti-inflammatory and keratin accumulation prevention effects of the pore cleaning composition to a certain extent; thereby affecting the removal effects of blackheads and whiteheads when it is applied to toner, and also affecting the pore shrinking effect; when the sum of the mass percentages of hydroxyl asiatic acid and matrine is further selected within the above range, the comprehensive effect of the obtained pore cleaning composition is better.

[0016] As a preferred embodiment of the pore cleaning composition of the present application, the mass percentage of coffea arabica seed extract is ≥7% based on the total mass of the pore cleaning composition.

[0017] Exemplarily, the mass percentage of coffea arabica seed extract can be any point value or any two-point range value between ≥7% based on the total mass of the pore cleaning composition, such as 7-14%, 7-19%, 7-34%, 7-38%, etc.; or it can be 7%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, 32%, 34%, 36%, 38%, etc.

[0018] Preferably, the mass percentage of coffea arabica seed extract is 7-14% based on the total mass of the pore cleaning composition.

[0019] The present application researches and finds that when the mass percentage of coffea arabica seed extract is further selected within the above range, the comprehensive effect of the obtained plant extract is better.

[0020] As a preferred embodiment of the pore cleaning composition of the present application, the preparation method of the medicinal Fomes fomentarius-Lactobacillus plantarum bidirectional fermentation product comprises the following steps:

[0021] (1) anaerobic fermentation after mixing medicinal Fomes fomentarius and Lactobacillus plantarum bacterial liquid, to obtain a fermentation product;

[0022] (2) after cooling the fermentation product to 2-6℃, circulating treatment in a microfluidizer homogenizer, finally crushing and centrifuging, collecting the supernatant and drying, to obtain a medicinal Fomes fomentarius-Lactobacillus plantarum bidirectional fermentation product.

[0023] The present application found that, by bidirectional fermentation of medicinal Fomes fomentarius and Lactobacillus plantarum in the above manner, medicinal Fomes fomentarius under the action of Lactobacillus plantarum not only has higher extraction efficiency, but also the bidirectional fermentation product obtained may produce additional active ingredients that were not present before fermentation, and it can better interact with other components, thereby improving the overall effect of the pore cleaning composition.

[0024] As a preferred embodiment of the pore cleaning composition of the present application, in step (1), the mass ratio of medicinal Fomes fomentarius and Lactobacillus plantarum bacterial liquid is 1:(4-6).

[0025] For example, the mass ratio of medicinal Fomes fomentarius and Lactobacillus plantarum bacterial liquid can be any point value or any two-point range value between 1:(4-6), such as 1:4, 1:5, 1:6, etc.

[0026] Preferably, in step (1), the mass ratio of medicinal Fomes fomentarius and Lactobacillus plantarum bacterial liquid is 1:5.

[0027] The present application speculates that during the preparation of the bidirectional fermentation product, the mass ratio of medicinal Fomes fomentarius and Lactobacillus plantarum bacterial liquid will affect the types and contents of active ingredients in the fermentation product to some extent, and when the mass ratio of the two is further selected within the above range, the bidirectional fermentation product obtained is applied to the subsequent pore cleaning composition, and the overall effect of the pore cleaning composition obtained is better.

[0028] It should be noted that the medicinal Fomes fomentarius further includes a pretreatment step before being mixed with the Lactobacillus plantarum fermentation liquid; the pretreatment of the medicinal Fomes fomentarius comprises crushing the dried fruiting body of medicinal Fomes fomentarius to 40-60 mesh and sterilizing.

[0029] As a preferred embodiment of the pore cleaning composition of the present application, in step (1), the temperature of anaerobic fermentation is 37±2℃, and the time of anaerobic fermentation is 48-72h.

[0030] Exemplarily, the time of the anaerobic fermentation can be any point value or any two-point range value between 48-72h, such as 48h, 60h, 72h, etc.

[0031] As a preferred embodiment of the pore cleaning composition of the present application, in step (1), 1-3% g / mL mass concentration of glucose and 0.03-0.07% g / mL mass concentration of magnesium sulfate are further added for anaerobic fermentation based on the total mass of the Phaeolus schweinitzii and Lactobacillus plantarum bacterial solution.

[0032] As a preferred embodiment of the pore cleaning composition of the present application, in step (2), the pressure of the circulation treatment is 1000-1500 bar, the temperature of the circulation treatment is <40℃, and the number of times of the circulation treatment is 3-5 times.

[0033] Preferably, in step (2), the pressure of the circulation treatment is 1200-1400 bar, and the temperature of the circulation treatment is 20-30℃.

[0034] Preferably, in step (2), the stirring speed in the microfluidic homogenizer circulation treatment is 500-800 rpm.

[0035] The present application found that, in the low-temperature microfluidic extraction process of step (2), the fermentation product is cooled first and then extracted within the above parameter range, which can improve the extraction efficiency and effectively extract active components that can synergize with other components, thereby improving the overall effect of the pore cleaning composition.

[0036] Preferably, the speed of the broken centrifugation is 10000-14000g, and the time is 10-30min.

[0037] As a preferred embodiment of the pore cleaning composition of the present application, the preparation method of the Lactobacillus plantarum bacterial solution comprises the following steps: inoculating Lactobacillus plantarum into MRS liquid medium for anaerobic fermentation to an OD600 value of 1.2-1.5 to obtain a Lactobacillus plantarum bacterial solution.

[0038] Preferably, the inoculation amount of the Lactobacillus plantarum is 1-5% (V / V).

[0039] Preferably, the temperature of the anaerobic fermentation is 37±2℃, and the time of the anaerobic fermentation is 18-24h.

[0040] Preferably, the Lactobacillus plantarum is any one of ATCC 14917 and ATCC 8014.

[0041] In the second aspect of the present application, the present application provides the use of the pore cleaning composition in the preparation of skin care products.

[0042] The pore cleaning composition provided by the present application can inhibit sebum oxidation, prevent keratin accumulation, and has anti-inflammatory and bactericidal effects, thereby effectively, quickly, gently and long-acting reducing blackheads and whiteheads and shrinking pores; thus, the pore cleaning composition can be widely applied in the preparation of skin care products.

[0043] As a preferred embodiment of the application, the skin care product comprises any one of cosmetic water, emulsion, cream, mask, serum, and spray.

[0044] In a third aspect of the present application, the present application provides a cosmetic water comprising the following components by mass percentage: 1-10% of the pore cleaning composition of the present application, 0.1-1% of a thickening agent, 0.5-5% of a humectant, 0.01-0.3% of a pH regulator, 0.5-3% of a preservative, and the balance of deionized water.

[0045] As a preferred embodiment of the cosmetic water of the present application, the cosmetic water comprises the following components by mass percentage: 4-6% of the pore cleaning composition of the present application, 0.2-0.5% of a thickening agent, 2.5-4% of a humectant, 0.03-0.08% of a pH regulator, 0.5-1.5% of a preservative, and the balance of deionized water.

[0046] As a preferred embodiment of the cosmetic water of the present application, the thickening agent comprises at least one of polyacrylate crosspolymer-6, carbomer, carrageenan, gellan gum, xanthan gum, microcrystalline cellulose, cellulose gum, ethyl cellulose, tara gum, guar gum, acryloyldimethyltaurine ammonium / VP copolymer, and acrylate copolymer.

[0047] As a preferred embodiment of the cosmetic water of the present application, the humectant comprises at least one of glycerin, D-panthenol, vitamin B5, 1,3-butanediol, 1,2-hexanediol, 1,3-propanediol, sodium hyaluronate, tremella fuciformis polysaccharide, trehalose, betaine, allantoin, and low-molecular-weight sodium hyaluronate.

[0048] As a preferred embodiment of the cosmetic water of the present application, the pH regulator comprises at least one of arginine, disodium ethylenediaminetetraacetate, tromethamine, and disodium EDTA.

[0049] As a preferred embodiment of the cosmetic water of the present application, the preservative comprises at least one of p-hydroxyacetophenone and polyhydric alcohol.

[0050] Compared with the prior art, the present application has the following beneficial effects:

[0051] The present application can realize rapid and long-acting reduction of blackheads and whiteheads and pore shrinking effect through the multi-pathway synergy of sebum oxidation inhibition, keratin accumulation prevention, anti-inflammation and sterilization, etc., by selecting appropriate mass fractions of components, and mutual interaction and cooperation among the components; meanwhile, the pore cleaning composition provided by the present application is mild and non-irritating, and is particularly suitable for sensitive skin people. BRIEF DESCRIPTION OF DRAWINGS

[0052] Figure 1 A comparison chart of blackhead and whitehead improvement in the nose area of volunteers before and after use in the blank application and application example 1 of the present application. DETAILED DESCRIPTION

[0053] For the purpose of better illustrating the present application, technical solutions and advantages, the present application will be further described below in combination with specific examples.

[0054] The reagents, methods and equipment used in the present application are all conventional reagents, methods and equipment in the field unless otherwise specified; and the raw materials used in parallel experiments are the same batch of raw materials unless otherwise specified.

[0055] Guaiacwood extract: purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0056] Coffea arabica seed extract: purchased from Xi'an Qingsuo Import & Export Trade Co., Ltd.

[0057] Madecassoside: purchased from Shaanxi Wanhe Pharmaceutical Co., Ltd., purity 98%.

[0058] Matrine: purchased from Shanxi Xinyu Biotechnology Co., Ltd., purity 98%.

[0059] Bidirectional fermentation product 1: self-made, the preparation method comprising the following steps:

[0060] (1) inoculate Lactobacillus plantarum (ATCC 8014) into MRS liquid medium (purchased from Thermo Fisher) at an inoculation amount of 3% (V / V), anaerobically ferment at 37℃ for 20h to an OD600 value of 1.4, and obtain Lactobacillus plantarum bacterial liquid;

[0061] (2) crush the dried fruiting bodies of Phellinus baumii (purchased from Bo Man Pharmaceutical Co., Ltd. in Anhui Province) to 40 mesh, sterilize at 121℃ for 15min, and obtain Phellinus baumii powder;

[0062] (3) mix the Phellinus baumii powder and the Lactobacillus plantarum bacterial liquid at a mass ratio of 1:5, and add 2% g / mL mass volume concentration of glucose and 0.05% g / mL mass volume concentration of magnesium sulfate based on the total volume of the Phellinus baumii powder and the Lactobacillus plantarum bacterial liquid, and then anaerobically ferment at 37℃ for 60h, and obtain the fermentation product;

[0063] (4) The fermentation product was pre-cooled to 4°C, then treated with a microfluidizer for 4 cycles at 1200 bar pressure, 600 rpm stirring speed, and 25°C temperature. After the end of the cycle treatment, the product was broken, then centrifuged at 12000 g for 20 min. The supernatant was collected and vacuum dried at 25°C to obtain the two-way fermentation product 1.

[0064] Two-way fermentation product 2: self-made, the preparation method comprising the following steps:

[0065] (1) Lactobacillus plantarum (ATCC 14917) was inoculated into MRS liquid medium at an inoculation amount of 3% (V / V), anaerobically fermented at 37°C for 18 h to an OD600 value of 1.2 to obtain a lactobacillus plantarum liquid;

[0066] (2) The dried fruiting body of Phellinus baumii (purchased from Bo Man Pharmaceutical Co., Ltd. in Anhui Province) was crushed to 40 mesh and sterilized at 121°C for 15 min to obtain Phellinus baumii powder;

[0067] (3) The Phellinus baumii powder and the lactobacillus plantarum liquid were mixed at a mass ratio of 1:6, and 2% g / mL mass-volume concentration of glucose and 0.05% g / mL mass-volume concentration of magnesium sulfate were added based on the total volume of the Phellinus baumii powder and the lactobacillus plantarum liquid, and then anaerobically fermented at 37°C for 72 h to obtain a fermentation product;

[0068] (4) The fermentation product was pre-cooled to 4°C, then treated with a microfluidizer for 5 cycles at 1400 bar pressure, 600 rpm stirring speed, and 20°C temperature. After the end of the cycle treatment, the product was broken, then centrifuged at 12000 g for 20 min. The supernatant was collected and vacuum dried at 25°C to obtain the two-way fermentation product 2.

[0069] Two-way fermentation product 3: self-made, the difference between the preparation method and that of the two-way fermentation product 1 is that the mass ratio of the Phellinus baumii powder and the lactobacillus plantarum liquid is 1:3.

[0070] Two-way fermentation product 4: self-made, the difference between the preparation method and that of the two-way fermentation product 1 is that the mass ratio of the Phellinus baumii powder and the lactobacillus plantarum liquid is 1:8.

[0071] Two-way fermentation product 5: self-made, the difference between the preparation method and that of the two-way fermentation product 1 is that the microfluidizer is used for 4 cycles at 1200 bar pressure and 45°C temperature.

[0072] Two-way fermentation product 6: self-made, the preparation method comprising the following steps:

[0073] (1) Lactobacillus plantarum (ATCC 8014) was inoculated into MRS liquid medium at an inoculation amount of 3% (V / V) and anaerobically fermented at 37℃ for 20h to an OD600 value of 1.4 to obtain a Lactobacillus plantarum bacterial solution;

[0074] (2) The dried fruiting bodies of Phellinus baumii (purchased from Bo Man Pharmaceutical Co., Ltd. in Anhui Province) were ground to 40 mesh and sterilized at 121℃ for 15min to obtain Phellinus baumii powder;

[0075] (3) The Phellinus baumii powder and the Lactobacillus plantarum bacterial solution were mixed at a mass ratio of 1:5, and 2% g / mL mass-volume concentration of glucose and 0.05% g / mL mass-volume concentration of magnesium sulfate were added based on the total volume of the Phellinus baumii powder and the Lactobacillus plantarum bacterial solution, followed by anaerobic fermentation at 37℃ for 60h to obtain a fermentation product;

[0076] (4) The fermentation product was centrifuged at a speed of 12000g for 20min, and the supernatant was collected and vacuum dried at 25℃ to obtain bidirectional fermentation product 6.

[0077] Bidirectional fermentation product 7: self-made, the difference from bidirectional fermentation product 1 is that Lactobacillus plantarum is replaced by Saccharomyces cerevisiae (ATCC204508).

[0078] Bidirectional fermentation product 8: self-made, the difference from bidirectional fermentation product 1 is that Phellinus baumii is replaced by Phellinus linteus (purchased from Shaanxi Haosun Biological Technology Co., Ltd.).

[0079] Examples 1-8 and Comparative Examples 1-8

[0080] The examples and comparative examples of the present application provide a pore cleaning composition, the components (mass parts) of which are shown in Table 1; wherein W1 represents the sum of the mass percentages of hydroxyl asperosaponin and matrine based on the total mass parts of the pore cleaning composition, and W2 represents the mass percentage of coffee arabica seed extract based on the total mass parts of the pore cleaning composition.

[0081] Table 1

[0082]

[0083]

[0084] The pore cleaning composition provided in Example 1 is prepared by mixing the components to obtain the pore cleaning composition.

[0085] The pore cleaning compositions provided in Examples 2-8 and Comparative Examples 1-8 are prepared in the same manner as in Example 1; if there is no related component, it is not added.

[0086] Example 9

[0087] The present example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 2 is substituted for Bidirectional Fermentation Product 1.

[0088] Example 10

[0089] The present example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 3 is substituted for Bidirectional Fermentation Product 1.

[0090] Example 11

[0091] The present example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 4 is substituted for Bidirectional Fermentation Product 1.

[0092] Example 12

[0093] The present example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 5 is substituted for Bidirectional Fermentation Product 1.

[0094] Example 13

[0095] The present example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 6 is substituted for Bidirectional Fermentation Product 1.

[0096] Comparative Example 9

[0097] The present comparative example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 7 is substituted for Bidirectional Fermentation Product 1.

[0098] Comparative Example 10

[0099] The present comparative example provides a pore cleansing composition that differs from Example 1 only in that Bidirectional Fermentation Product 8 is substituted for Bidirectional Fermentation Product 1.

[0100] Comparative Example 11

[0101] The present comparative example provides a pore cleansing composition that differs from Example 1 only in that Brazilian Aniba roseodora extract (purchased from Shaanxi Lvshengyuan Bioproducts Manufacturing Co., Ltd.) is substituted for guaiac wood extract.

[0102] Comparative Example 12

[0103] The present application comparative example provides a pore cleaning composition, the only difference between the pore cleaning composition and example 1 is that the madecassic acid (purchased from Shaanxi Wanhe Pharmaceutical Co., Ltd.) is used to replace the madecassoside.

[0104] Comparative example 13

[0105] The present application comparative example provides a pore cleaning composition, the only difference between the pore cleaning composition and example 1 is that the cocoa seed extract (purchased from Xi'an Shouhe Biological Technology Co., Ltd.) is used to replace the coffea arabica seed extract.

[0106] Comparative example 14

[0107] The present application comparative example provides a pore cleaning composition, the only difference between the pore cleaning composition and example 1 is that the madecassic acid (purchased from Shaanxi Wanhe Pharmaceutical Co., Ltd.) is used to replace the madecassoside.

[0108] The present application example, comparative example and blank example provide a cosmetic water, the components (mass percentage) of the cosmetic water are shown in Table 2; wherein the pore cleaning composition used in application examples 1-13 is respectively prepared in examples 1-13, for example, the pore cleaning composition used in application example 1 is the pore cleaning composition in example 1, the pore cleaning composition used in application example 2 is the pore cleaning composition in example 2, and so on; the pore cleaning composition used in comparative application examples 1-14 is respectively prepared in comparative examples 1-14; the pore cleaning composition used in application examples 14-15 is the pore cleaning composition prepared in example 1.

[0109] Table 2

[0110]

[0111]

[0112] The preparation method of the cosmetic water provided by application example 1 includes the following steps:

[0113] (1) The humectant and thickening agent are mixed with water and stirred, heated to 85℃, then homogenized at a speed of 1300rpm for 4min to obtain a pre-prepared A component;

[0114] (2) The preservative is mixed and melted at 60℃ to obtain a pre-prepared B component;

[0115] (3) After pre-prepared A component is cooled to 60℃, pre-prepared B component is added to stir and mix at a rotating speed of 300 rpm, then cooled to below 45℃, the pore cleaning composition is added to continue stirring for 8 min, finally the pH regulator is added to adjust the pH to 5.5-6.0, then the stirring is stopped, the product is discharged, and the cosmetic water is obtained.

[0116] The preparation method of the emulsion provided in Application Examples 2-15, Comparative Application Examples 1-14 and Blank Application Example, and Application Example 1 is consistent, and no relevant components are added.

[0117] Effect Example 1

[0118] The effects of the mood beauty composition prepared in the Effect Examples and the Comparative Examples 1-14 and 1-13 are explored, including the following aspects:

[0119] 1. Inhibition of sebum oxidation in vitro

[0120] In this test, the thiobarbituric acid reaction (TBARs) method is used to evaluate the inhibition of sebum oxidation and the ability to remove blackheads. The end product of sebum oxidation is mainly malondialdehyde. These peroxidation products react with thiobarbituric acid to form a colored compound, which has an absorption peak at 530 nm. Therefore, the level of sebum oxidation can be evaluated by detecting the absorbance at 530 nm.

[0121] The test method is as follows:

[0122] (1) Preparation of artificial sebum: Squalene, triglyceride, and wax are mixed in a ratio of 4:3:3 to obtain artificial sebum;

[0123] (2) Preparation of thiobarbituric acid solution: 0.1 mol / L hydrochloric acid aqueous solution and 1% thiobarbituric acid aqueous solution are mixed in a volume ratio of 2:1, heated to boiling until the thiobarbituric acid is completely dissolved, and stored for use. The thiobarbituric acid solution is prepared.

[0124] (3) The pore cleaning composition prepared in the examples and comparative examples (blank control group without adding composition) is added to the artificial sebum (the mass percentage of the pore cleaning composition is 1% based on the mass of the artificial sebum), mixed uniformly, and incubated at 75℃, 60% RH, and light for 24 hours.

[0125] (4) The incubated reaction solution is mixed with the thiobarbituric acid solution in a volume ratio of 7:3, heated in a 65℃ water bath for 1 hour, centrifuged to obtain the supernatant, and the absorbance OD value is measured at 530 nm.

[0126] Sebum is easily oxidized and darkened in the air, and eventually forms blackheads. Therefore, the lower the oxidation level of sebum, the stronger the ability of the sample to inhibit the oxidation of sebum, and the stronger the ability to remove blackheads. The ability of the composition to remove blackheads is represented by the decrease in the oxidation level of sebum, and the calculation formula is as follows: Decrease rate of sebum oxidation level (%) = (1 - OD value of sample group / OD value of blank control group) * 100%;

[0127] The results obtained are shown in Table 3.

[0128] 2. Ability to prevent keratinocyte accumulation in vitro

[0129] Experimental materials: The cell line used was human keratinocytes HaCaT (Guangzhou Jiniuo Biological Technology Co., Ltd.), and the test conditions were: incubator temperature 37±1℃, humidity 90±5%, carbon dioxide 5±1%; the cells were cultured and treated according to the grouping, and then tested, and the specific test was keratin K10 expression detection.

[0130] The specific test method is as follows:

[0131] (1) The cell suspension was inoculated in a 96-well cell culture plate at a density of 2000 cells / well, and 100 μL of culture medium was added to each well, and cultured for 24 h;

[0132] (2) Discard the supernatant, add 100 μL of DMEM medium (2105341, Gibco) to the blank control group, and add 100 μL of medium containing 1% g / mL of the corresponding sample (pore cleaning composition prepared in the examples and comparative examples) to the sample group, and continue to culture for 48 h, then collect the cells;

[0133] (3) Keratin K10 content detection: The cells were lysed with RIPA cell lysis buffer containing protease inhibitors, and the lysed sample was centrifuged at 12000g for 5 minutes, and the supernatant was taken, and the human keratin 10 (KRT10) ELISA detection kit (item number JL32475-96T, Jianglai Biological) was used to detect the keratin K10 content of the supernatant of each group of cells.

[0134] Excessive accumulation of keratinocytes can cause comedones, pore blockage, and oil accumulation inside the pores to form whiteheads. Keratin K10 is mainly expressed during the differentiation period of keratinocytes and participates in the formation of a stable intermediate fiber network to provide mechanical strength to the stratum corneum and ensure the orderly shedding of keratinocytes. Therefore, the higher the expression of keratin K10, the stronger the ability of the sample to remove whiteheads, and the lower the expression of keratin K10, the weaker the ability of the sample to remove whiteheads. The ability of the composition to remove whiteheads is represented by the increase in keratin K10, and the calculation formula is as follows: Keratin K10 increase rate = (sample group - blank control group) / blank control group x 100%;

[0135] The results obtained are shown in Table 3.

[0136] Table 3

[0137]

[0138] As can be seen from Table 3, when the technical solution provided by the present application is used, the obtained pore cleaning composition has good ability to reduce sebum oxidation and prevent keratinocyte accumulation; specifically, the obtained product has a sebum oxidation reduction rate of more than 39.5% and a keratin K10 increase rate of more than 19.8%;

[0139] As can be seen from Examples 1-8, the mass parts of the components and the mass ratio between the components or the mass percentage of a certain component or two components in the composition can also affect the performance of the product. When the mass parts of the components are further selected within the preferred range given by the present application, the comprehensive performance of the obtained product is better; specifically, the obtained product has a sebum oxidation reduction rate of more than 77.9% and a keratin K10 increase rate of more than 40.9%; As can be seen from Examples 1 and Comparative Examples 9-13, the preparation method of the two-way fermentation product can also affect the comprehensive performance of the product to some extent;

[0140] As can be seen from Examples 1 and Comparative Examples 1-8, when one or two components are not added, or when the other components are added to make up for the missing components, the effect of the present application cannot be achieved; As can be seen from Examples 1 and Comparative Examples 9-10, when the raw material of the two-way fermentation product is not the substance provided by the present application, the effect of the present application cannot be achieved; As can be seen from Examples 1 and Comparative Examples 11-14, when other similar components are used instead, the corresponding effect of the present application cannot be achieved.

[0141] Example 2

[0142] The safety of the cosmetic water prepared from the effect example, application example 1-15, comparative application example 1-14 and blank application example is explored, specifically, human skin patch test is used for testing:

[0143] 30 volunteers, 15 males and 15 females, aged 20-50 years old, are recruited, and a closed patch test method is used, equal amounts (0.020 mL-0.025 mL) of test samples (cosmetic water prepared from the application examples) are placed in a specific patch tester, and are attached to the arms of the volunteers with a low-sensitization adhesive tape, and are lightly pressed to be evenly attached to the skin, and are left for 24 hours; the blank control group is distilled water. After 24 hours, the patch tester is removed, and the skin reaction is observed after 0.5 hours, 24 hours and 48 hours, and the results are recorded. The adverse reaction grade of the skin is shown in Table 4 below;

[0144] Table 4

[0145]

[0146] The results show that the cosmetic water prepared from the application example 1-15, comparative application example 1-14 and blank application example is negative after human patch test, and is safe and non-irritating to human skin.

[0147] Effect Example 3

[0148] The application effect of the cosmetic water prepared from the application example 1-15, comparative application example 1-14 and blank application example on human body is explored, including the following steps:

[0149] 180 Asian adult testers aged 18-60 years old with obvious blackheads, whiteheads and coarse pores on the sensitive skin are selected according to the test in the “Cosmetic Safety Technical Specification” (2015), and are randomly divided into 30 groups, 6 people in each group. The volunteers use the sample (cosmetic water prepared from the application example, comparative application example and blank application example) on the whole face once a day in the morning and evening after regular cleansing, and visit and collect data on the 0th day, 7th day, 28th day and 5 days after stopping use (5 days after stopping use means that the corresponding cosmetic water is not used after regular cleansing). After the volunteers visit, they wash their faces with facial cleanser, and sit in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes. The researchers use VISIA-CR to take pictures of the volunteers' faces for analysis, and calculate the number of blackheads and whiteheads according to the images; the skin image analyzer digimiC800 is used to measure the pore diameter of the skin outside the nasolabial sulcus.

[0150] The blackhead removal ability of the composition is represented by the reduction of the number of blackheads, the whitehead removal ability is represented by the reduction of the number of whiteheads, and the pore shrinking ability is represented by the reduction of the pore diameter, and the formula is as follows:

[0151] Improvement rate = (T0 样品组-Tx 样品组 ) / T0 样品组 *100%]-[(T0 空白应用例 -Tx 空白应用例 ) / T0 空白应用例 *100%];

[0152] The results are shown in Table 5.

[0153] Table 5

[0154]

[0155]

[0156] As can be seen from Table 5, when the technical solution provided by the present application is adopted, the obtained toner has good effects of reducing blackheads and whiteheads and shrinking pores, and the obtained product has rapid and lasting effects of reducing blackheads and whiteheads and shrinking pores; specifically, the improvement rates of the obtained toner on blackheads and whiteheads are 12.9% and 14.9% or more at T7, and the improvement rate on pores is 11.5% or more; the improvement rates on blackheads and whiteheads are 29.7% and 34.9% or more at T28, and the improvement rate on pores is 20.6% or more; the improvement rates on blackheads and whiteheads are 11.4% and 14.1% or more at T5, and the improvement rate on pores is 11.1% or more.

[0157] As can be seen from Application Examples 1-8, the mass parts of the components in the pore cleaning composition added in the emulsion and the mass ratio between the components or the mass percentage of a certain component or two components in the composition will also affect the performance of the toner, and when the mass parts of the components in the pore cleaning composition are further selected within the preferred range given by the present application, the comprehensive performance of the obtained toner is better; specifically, the improvement rates of the obtained toner on blackheads and whiteheads are 53.9% and 60.6% or more at T7, and the improvement rate on pores is 32.3% or more; the improvement rates on blackheads and whiteheads are 60.6% and 69.2% or more at T28, and the improvement rate on pores is 34.4% or more; the improvement rates on blackheads and whiteheads are 49.9% and 56.1% or more at T5, and the improvement rate on pores is 31.1% or more.

[0158] As can be seen from Application Example 1 and Application Examples 9-13, the preparation method of the two-way fermentation product will also affect the comprehensive performance of the toner to some extent.

[0159] As can be seen from the application example 1 and the comparative application examples 1-8, when one or two components are not added in the pore cleaning composition, or when the components are not added while the fractions of other components are supplemented, the effects of the present application cannot be achieved; as can be seen from the application example 1 and the comparative application examples 9-14, when other similar components are used to replace the raw materials in the bidirectional fermentation product or other similar components are used to replace other components, the corresponding effects of the present application cannot be achieved.

[0160] At the same time, a volunteer was randomly selected from the application example 1 and the blank application example to give the test pictures before and after use, as shown in Figure 1 , wherein the black arrow represents the position of blackheads, and the red arrow represents the position of whiteheads; as can be obviously seen from Figure 1 , the cosmetic lotion prepared by using the present application can effectively reduce blackheads and whiteheads after 28 days of use.

[0161] Finally, it should be noted that the above examples are used to illustrate the technical solutions of the present application, but not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A pore-cleansing composition containing medicinal Fomes fomentarius, characterized by, The pore cleaning composition is composed of the following components in mass parts: bidirectional fermentation product 0.5-5 parts, guaiacum extract 0.5-3 parts, hydroxyl asperosaponin 0.05-0.5 parts, coffee arabica seed extract 0.1-1 part, and matrine 0.05-0.5 part; The bidirectional fermentation product is a bidirectional fermentation product of phellinus baumii and lactobacillus plantarum; The sum of the mass percentages of hydroxyl asperosaponin and matrine is 1-34% based on the total mass of the pore cleaning composition; The mass percentage of coffee arabica seed extract is ≥7% based on the total mass of the pore cleaning composition; The preparation method of the bidirectional fermentation product of phellinus baumii and lactobacillus plantarum comprises the following steps: (1) mixing phellinus baumii and lactobacillus plantarum bacterial liquid and then anaerobic fermentation to obtain a fermentation product; (2) cooling the fermentation product to 2-6℃, then circulating treatment in a microfluidizer, finally crushing and centrifuging, collecting the supernatant and drying to obtain the bidirectional fermentation product of phellinus baumii and lactobacillus plantarum.

2. The pore cleansing composition according to claim 1, characterized by, The pore cleaning composition is composed of the following components in mass parts: bidirectional fermentation product 1-2 parts, guaiacum extract 1-2 parts, hydroxyl asperosaponin 0.1-0.2 parts, coffee arabica seed extract 0.3-0.5 part, and matrine 0.2-0.3 part.

3. The pore-cleansing composition according to claim 1, wherein In the step (1), the mass ratio of phellinus baumii to lactobacillus plantarum bacterial liquid is 1:(4-6); And / or, in the step (1), the temperature of anaerobic fermentation is 37±2℃, and the time of anaerobic fermentation is 48-72h; And / or, in the step (1), 1-3% g / mL mass volume concentration of glucose and 0.03-0.07% g / mL mass volume concentration of magnesium sulfate are further added for anaerobic fermentation based on the total volume of phellinus baumii and lactobacillus plantarum bacterial liquid.

4. The comedolytic composition of claim 1, wherein In the step (2), the pressure of circulating treatment is 1000-1500 bar, the temperature of circulating treatment is <40℃, and the number of circulating treatment is 3-5 times.

5. The comedolytic composition of claim 1, wherein The preparation method of the lactobacillus plantarum bacterial liquid comprises the following steps: inoculating lactobacillus plantarum into MRS liquid medium and anaerobic fermentation to an OD600 value of 1.2-1.5 to obtain the lactobacillus plantarum bacterial liquid.

6. Use of the pore cleaning composition according to any one of claims 1-5 in the preparation of a skin care product.

7. A cosmetic water, characterized by, The cosmetic water comprises the following components in mass percentages: 1-10% of the pore cleaning composition according to any one of claims 1-5, 0.1-1% of a thickening agent, 0.5-5% of a humectant, 0.01-0.3% of a pH adjuster, 0.5-3% of a preservative, and the balance of deionized water.

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