A composition for oil control and maintenance of scalp microflora, use and cosmetic

Abstract

The application belongs to the field of daily chemicals, and discloses a composition for controlling oil and maintaining scalp microecology, which comprises a silk fibroin peptide-containing solution, a prebiotic composition and polylysine; the weight ratio of the silk fibroin peptide-containing solution, the prebiotic composition and the polylysine is 3-20:5-10:0.1-1. The composition combines polylysine and prebiotics (inulin, fructooligosaccharide) with silk fibroin peptide (containing lysozyme) to achieve better oil control performance, and meanwhile, the composition utilizes the functions of silk fibroin peptide such as moisturizing and barrier repair to establish a complete skin barrier while controlling harmful bacteria, so that the microecology is repaired and improved. Meanwhile, the application also provides application and cosmetics of the composition.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of daily chemicals, in particular to a composition for controlling oil and maintaining scalp microecology. BACKGROUND

[0002] Lysozyme and polylysine are two classic antibacterial agents in cosmetics, which are widely used for antibacterial purposes, as shown in the following documents:

[0003] The application with the publication number CN118453445A and the subject of a probiotic oral care bacteriostatic agent and its preparation method discloses a probiotic oral care bacteriostatic agent, which comprises water, oligofructose, polydextrose, inulin, stachyose, tea polyphenol, epsilon-polylysine hydrochloride, lysozyme, and flavoring agent. It is further pointed out that the bacteriostatic components use epsilon-polylysine hydrochloride, lysozyme, and tea polyphenol. The probiotic solution is prepared by adding "inulin, oligofructose, polydextrose, stachyose", and pure water. The probiotic can be used by Streptococcus salivarius and other beneficial bacteria in the oral cavity, so that the bacteria proliferate to become dominant bacteria, thereby inhibiting the activity of harmful bacteria and improving the antibacterial property of the oral care bacteriostatic agent.

[0004] In the field of skin and hair-related care, the application with the publication number CN110917074A and the subject of a composition with antibacterial effect and its application in additive-free skincare products discloses a composition with antibacterial effect. Through the appropriate selection of nisin, polylysine, and cordycepin, and the appropriate selection of the amount, the prepared composition has good antibacterial and bacteriostatic effects, especially for heat-resistant coliform bacteria, Pseudomonas aeruginosa, Streptococcus albus, Aspergillus niger, and yeast.

[0005] The purpose of the present application is to develop a composition that can act on the scalp microecology maintenance and improvement. Based on the functions of lysozyme, polylysine, and probiotics (inulin, oligofructose), we further develop the composition. SUMMARY

[0006] The purpose of the present application is to provide a composition for controlling oil and maintaining scalp microecology. The composition uses polylysine and probiotics (inulin, oligofructose) combined with sericin peptide (containing lysozyme) to achieve better oil control performance. At the same time, the composition utilizes the functions of sericin peptide such as moisturizing and barrier repair to establish a complete skin barrier while controlling harmful bacteria, thereby achieving the repair and improvement of the microecology.

[0007] Meanwhile, the present application also provides the application and cosmetics of the composition.

[0008] To achieve the above-mentioned purpose, the present application discloses a composition for controlling oil and maintaining scalp microecology, which comprises a solution containing sericin peptide, a probiotic composition, and polylysine.

[0009] The weight ratio of the silk fibroin peptide-containing solution, the prebiotic composition and the polylysine is 3-20:5-10:0.1-1;

[0010] The content of the silk fibroin peptide in the silk fibroin peptide-containing solution is 5 wt%, and the product provided by Myogen Biotech (Shanghai) Co., Ltd. and named InnoSeriCARE is used.

[0011] In the above composition, the prebiotic composition is fructo-oligosaccharide and / or inulin.

[0012] In the above composition, the prebiotic composition is a combination of fructo-oligosaccharide and inulin; and the weight ratio of the inulin and the fructo-oligosaccharide is 1:10-1:1.

[0013] In the above composition, the weight ratio of the inulin and the fructo-oligosaccharide is 1:4-2:5.

[0014] Meanwhile, the application also discloses the use of the above composition for preparing cosmetics.

[0015] Meanwhile, the application also discloses the use of the above composition for preparing antibacterial agents.

[0016] Finally, the application also discloses a cosmetic containing the above composition.

[0017] In the above cosmetic, the cosmetic is shampoo, shower gel or hair conditioner.

[0018] In the above cosmetic, the cosmetic contains 0.1%-10% of the composition.

[0019] The application has the following beneficial effects:

[0020] 1. The application combines silk fibroin peptide, prebiotic composition and polylysine, and through detection, it is found that the combination has very excellent oil control effect.

[0021] 2. The sample of the application is subjected to antibacterial detection of Staphylococcus aureus, Staphylococcus epidermidis and Malassezia furfur, and the detection result proves that the antibacterial effect on Malassezia furfur is extremely strong, the antibacterial effect on Staphylococcus aureus is relatively strong, and there is no antibacterial effect on Staphylococcus epidermidis; it is proved that the composition has good antibacterial effect and scalp micro-ecological restoration ability.

[0022] 3. The sample of the application is subjected to safety test, and it is proved that the sample is a mild product and can be used with confidence. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 Cell activity test results for Example 1 of the present invention;

[0024] Figure 2 Cell activity test results for Example 2 of the present invention;

[0025] Figure 3 Cell activity test results for Example 3 of the present invention;

[0026] Figure 4 Cell activity test results for Comparative Example 1 of the present invention;

[0027] Figure 5 Cell activity test results for Comparative Example 2 of the present invention;

[0028] Figure 6 Cell activity test results for Comparative Example 3 of the present invention;

[0029] Figure 7 Nile red staining fluorescence image for Example 1 of the present invention;

[0030] Figure 8 Nile red staining fluorescence image for Example 2 of the present invention;

[0031] Figure 9 Nile red staining fluorescence image for Example 3 of the present invention;

[0032] Figure 10 Nile red staining fluorescence image for Comparative Example 1 of the present invention;

[0033] Figure 11 Nile red staining fluorescence image for Comparative Example 2 of the present invention;

[0034] Figure 12 Nile red staining fluorescence image for Comparative Example 3 of the present invention;

[0035] Figure 13 Staphylococcus aureus inhibition chart for Example 2 of the present invention;

[0036] Figure 14 Staphylococcus epidermidis inhibition chart for Example 2 of the present invention;

[0037] Figure 15 Malassezia furfur inhibition chart for Example 1 of the present invention;

[0038] Figure 16 Malassezia furfur inhibition chart for Example 2 of the present invention;

[0039] Figure 17 Chicken embryo chorioallantoic membrane test chart for Example 1 of the present invention;

[0040] Figure 18 Figure for chicken embryo chorioallantoic membrane test of Example 2 of the present application. DETAILED DESCRIPTION

[0041] The present application will be described in detail below with reference to examples of the present application. In the description of the present application, it should be noted that, if specific conditions are not specified in the examples, conventional conditions or conditions recommended by the manufacturer are used. If the manufacturer of the reagent or instrument is not specified, it is a conventional product that can be purchased on the market.

[0042] Repair silk fibroin peptide: provided by Myogen Huobi (Shanghai) Biotechnology Research Co., Ltd., trade name InnoSeriCARE, related preparation technology reference CN112194715B Anti-inflammatory silk fibroin peptide and its application; the effective component of the product is silk fibroin peptide, and the effective component content is 5%.

[0043] Polylysine, oligofructose, inulin: provided by Guangzhou Heimao Biotechnology Co., Ltd., the effective content is 100%

[0044] Part I Examples and Comparative Examples

[0045] Polylysine, oligofructose, inulin, and hydrolyzed silk fibroin were mixed and dispersed in water to form a uniform aqueous phase. The weight of each effective component in the aqueous phase was referred to Table 1, and finally water was added to the total weight of the sample to 100g.

[0046] Table 1 Formulation Table (unit: g)

[0047] Repair silk fibroin peptide Inulin Fructo-oligosaccharides Polylysine Example 1 3 1 4 1 Example 2 8 2 5 0.5 Example 3 20 4 6 0.1 Comparative Example 1 20 0 0 0 Comparative Example 2 0 1 4 0 Comparative Example 3 0 0 0 1

[0048] Performance test

[0049] I. Oil control performance test

[0050] The samples of Examples 1-3 and Comparative Examples 1-3 were sent to Guangzhou Huaimao Biotechnology Research Institute Co., Ltd. for testing, and the test report numbers were 2504170-01A to 2504170-06A.

[0051] Purpose and principle of the test

[0052] SZ95 cell is an immortalized human facial sebaceous gland cell line, and the large amount of lipid secreted by human sebaceous gland cells is a prerequisite for the occurrence of acne. The sebaceous glands of acne patients secrete profusely. Linoleic acid-induced human sebaceous gland cells over-secreted lipid can be used as a cell model for studying the oil control ability of cosmetics. After human sebaceous gland cells are induced by linoleic acid and administered, the intracellular lipid deposition is observed under a microscope by Nile red staining. The change in intracellular fluorescence intensity after the negative control and the test sample are administered is compared to evaluate whether the test sample has oil control efficacy.

[0053] In this test, the laboratory method is used. The intracellular lipid content of human sebaceous gland cells is increased by linoleic acid induction. The change in fluorescence intensity of intracellular fluorescent dye after the negative control and the test sample are administered is compared to calculate the inhibition rate of intracellular lipid synthesis. If the intracellular lipid content of the test sample is lower than that of the negative control and has a significant difference (P<0.05), it is considered that the test sample has oil control efficacy.

[0054] Test materials and methods

[0055] Instrument equipment: MF52-N inverted fluorescence microscope;

[0056] Cells used in the experiment: human sebaceous gland cells (SZ95 cells);

[0057] Test method

[0058] (1) Treatment of test samples

[0059] Sample group: dilute the sample with pure water to a 4% solution, then filter it with a 0.22 μm filter, and collect the filtrate as the sample mother liquor.

[0060] (2) Test grouping

[0061] Negative control group (NC): cells (+) linoleic acid (+) Nile red (+) sample (-);

[0062] Positive control group (PC): cells (+) linoleic acid (+) Nile red (+) isotretinoin (+);

[0063] Sample group (S): cells (+) linoleic acid (+) Nile red (+) sample (+);

[0064] (3) Test operation steps

[0065] Cell activity test: human sebaceous gland cells were plated in a 96-well plate. After 24 h, the culture medium was aspirated, and the basic culture medium containing different concentrations of test samples was added. After 24 h, the OD490nm was detected by MTT method, and the effect of the test sample on the activity of human sebaceous gland cells was analyzed by t test.

[0066] Intracellular lipid synthesis inhibition rate determination: except for the blank group (BC) cell hole, the rest of the holes were added with a certain concentration of linoleic acid culture medium, and placed in a CO2 incubator for 6h. After the test group was dosed, the hole plate was placed in a CO2 incubator for 24h. After incubation, the supernatant was discarded, and the prepared Nile red staining solution was added to the hole for cell staining. Under the FITC channel, excitation wavelength 552nm, objective lens 20 times, set the same exposure time, and take pictures under the inverted fluorescence microscope.

[0067] Calculation formula

[0068] Intracellular lipid synthesis inhibition rate (%) = (1-T / C)*100%;

[0069] In the formula:

[0070] T—sample group / positive control fluorescence intensity average value;

[0071] C—negative control group fluorescence intensity average value.

[0072] Test results

[0073] Cell activity test

[0074] Before the intracellular lipid synthesis inhibition rate determination of the test sample was performed, the cell activity screening was performed first to obtain the best test concentration;

[0075] The cell activity test results of Example 1 to Example 3, Comparative Example 1 to Comparative Example 3 can be referred to Figures 1 to 6 ;

[0076] Nile red staining fluorescence map

[0077] The Nile red staining fluorescence map of Example 1 to Example 3, Comparative Example 1 to Comparative Example 3 can be referred to Figures 7 to 12 ;

[0078] Oil control test results

[0079] The experimental results are shown in Table 2;

[0080] Table 2 Oil control test results

[0081] Positive control group (PC) Sample group (0.125%) Sample group (0.25%) Example 1 59.90±1.15 14.13±2.51 11.87±1.30 Example 2 59.90±1.15 13.38±2.37 23.81±1.22 Example 3 59.60±1.84 4.40±1.85 25.28±1.32 Comparative Example 1 58.48±2.08 -3.30±1.52 5.68±2.77 Comparative Example 2 59.60±1.84 0.83±2.83 -0.45±1.76 Comparative Example 3 58.48±2.08 -9.36±1.51 -8.35±1.33

[0082] Result analysis:

[0083] 1. As can be seen from Comparative Example 1 to Comparative Example 3, each component used alone has no obvious oil control performance;

[0084] 2. From Examples 1 to 3, it can be seen that when the amount of polylysine is small (Example 3), after dilution to 0.125%, its oil control performance is obviously weaker than that of Examples 1 and 2, which shows that when the proportion of polylysine is reduced, the total concentration of the formula should be appropriately increased to ensure its full effectiveness;

[0085] 3. From Examples 1 to 3, it can be seen that the combination of the present application has very good oil control effect.

[0086] II. Staphylococcus aureus Bacteriostatic Test

[0087] Example 2 was taken as the sample, and the sample bacteriostatic rate was tested according to QB / T 2738-2023 Evaluation Method for Antimicrobial and Bacteriostatic Effect of Daily Chemical Products, and the test report number was 2504111-02AR1.

[0088] The test results showed that the blank control was <10 CFU / mL, the control sample group of Staphylococcus aureus was 3.9*10 4 CFU / mL, the system was effective, when the sample was diluted into a 1% aqueous solution, the Staphylococcus aureus was 1.3*10 4 CFU / mL, the inhibition rate was 66.67%, and the product had bacteriostatic effect.

[0089] The test results can be seen in the attached Figure 13 .

[0090] III. Staphylococcus epidermidis Bacteriostatic Test

[0091] Example 2 was taken as the sample, and the sample bacteriostatic rate was tested according to QB / T 2738-2023 Evaluation Method for Antimicrobial and Bacteriostatic Effect of Daily Chemical Products, and the test report number was 2504111-02BR1.

[0092] The test results showed that the blank control was <10 CFU / mL, the control sample group of Staphylococcus aureus was 3.9*10 4 CFU / mL, the system was effective, when the sample was diluted into a 1% aqueous solution, the Staphylococcus aureus was 1.3*10 4 CFU / mL, the inhibition rate was 66.67%, and the product had bacteriostatic effect.

[0093] The test results can be seen in the attached Figure 14 .

[0094] IV. Malassezia furfur Bacteriostatic Test

[0095] Examples 1 and 2 were taken as samples for testing; the test report numbers were 2504111-02AR1 and 2504168-02AR1.

[0096] Purpose and principle of the test

[0097] Dandruff problem is a large amount of desquamation phenomenon caused by excessive proliferation of keratinocytes on the scalp, among which Malassezia is one of the important factors causing dandruff. The increase of dandruff is a mild form of seborrheic dermatitis of the scalp. Due to the proliferation of Malassezia, the scalp is oily and the dandruff is severe, which in turn promotes the proliferation of Malassezia. Therefore, the addition of antidandruff agents to inhibit or kill such microorganisms has a positive effect on inhibiting the growth of dandruff. The dandruff effect of the shampoo product is tested and evaluated by taking Malassezia as an indicator. The test method is an in vitro method, which is suitable for evaluating the efficacy of dandruff cosmetics.

[0098] Experimental method

[0099] (1) Sample treatment

[0100] Sample: diluted with sterile standard hard water to a sample concentration of 0.5%;

[0101] Blank control: phosphate buffer;

[0102] Control sample group: Malassezia suspension + phosphate buffer.

[0103] (2) Test steps

[0104] Take the Malassezia suspension and add it to each of the test sample liquid and the control sample liquid, mix well, start timing, and when the specified action time is reached, dilute appropriately. Take 2-3 dilutions, respectively, and place the test sample liquid and the control sample liquid in two plates, pour the Malassezia culture medium and mix well, then turn the plate after it solidifies, and place it in a culture box for 5-10 days for viable bacteria count.

[0105] (3) Calculation formula

[0106] Malassezia furfur inhibition rate = (A-B) / A*100%

[0107] Wherein: A - average colony count of the control sample;

[0108] B - average colony count of the test sample.

[0109] The test results of Example 1 are as follows: the blank control is <10 CFU / mL, the control sample group Malassezia furfur is 4.5*10 4 CFU / mL, the system is effective, when the sample is diluted to 0.5% aqueous solution, the Malassezia furfur is 1.8*10 3 CFU / mL, the inhibition rate is 96.30%, indicating that the sample has certain antidandruff efficacy. The test results chart of Example 1 can be seen in Figure 15 .

[0110] The test result of Example 2 is: the blank control is <10 CFU / mL, the control sample group of Malassezia furfur is 4.5*10 4 CFU / mL, the system is effective, when the sample is diluted into 1% aqueous solution, the Malassezia furfur is 1.9*10 2 CFU / mL, the inhibition rate is 99.58%, which indicates that the sample has a certain anti-dandruff effect. The test result graph of Example 2 can be seen Figure 16 .

[0111] V. Irritation experiment

[0112] The samples of Example 1 and Example 2 were subjected to chicken embryo chorioallantoic membrane test, and the test report numbers were 2504168-01BR1, 2504168-02BR1;

[0113] The test result of Example 1 is: using the endpoint evaluation method, the negative control (0.9% physiological saline) ES = 0.00, indicating that the sample is non-irritating, meeting the standard of the negative control sample; the positive control (1% SDS) ES = 18.00, indicating that the sample is strongly irritating / corrosive, meeting the standard of the positive control sample; when the sample is diluted into a 0.5% aqueous solution, ES = 2.33, indicating that this sample is non-irritating, indicating that the sample passes the mild non-irritating test.

[0114] The test result of Example 2 is: using the endpoint evaluation method, the negative control (0.9% physiological saline) ES = 0.00, indicating that the sample is non-irritating, meeting the standard of the negative control sample; the positive control (1% SDS) ES = 18.00, indicating that the sample is strongly irritating / corrosive, meeting the standard of the positive control sample; when the sample is diluted into a 1% aqueous solution, ES = 2.00, indicating that this sample is non-irritating, indicating that the sample passes the mild non-irritating test.

[0115] Summary:

[0116] 1. The formula of the present application passes the oil control test, indicating that there is obvious oil control synergy between the components of the formula of the present application.

[0117] 2. The sample of the present application is subjected to antibacterial detection of Staphylococcus aureus, Staphylococcus epidermidis and Malassezia furfur, and the detection result confirms that the antibacterial effect on Malassezia furfur is extremely strong, the antibacterial effect on Staphylococcus aureus is relatively strong, and there is no antibacterial effect on Staphylococcus epidermidis; it is proved that the composition of the present application has good antibacterial effect and scalp microecological repair ability.

[0118] 3. The sample of the present application is subjected to safety test, and it is proved that it is a mild product and can be used with confidence.

Claims

1. A composition for oil control and maintenance of scalp microflora, characterized in that, A solution containing sericin peptide, a prebiotic composition, polylysine; The weight ratio of the solution containing sericin peptide, the prebiotic composition, and the polylysine is 3-20:5-10:0.1-1; The content of sericin peptide in the solution containing sericin peptide is 5wt%; The prebiotic composition is a combination of oligofructose and inulin; the weight ratio of the inulin and the oligofructose is 1:10-1:

1.

2. The composition of claim 1, wherein, The weight ratio of the inulin and the oligofructose is 1:4-2:

5.

3. Use of the composition according to claim 1 or 2 for preparing a cosmetic product.

4. Use of the composition according to claim 1 or 2 for preparing an antibacterial agent.

5. A cosmetic product, characterized by, The cosmetic product contains the composition according to claim 1 or 2.

6. The cosmetic product according to claim 5, characterized in that, The cosmetic product is shampoo, shower gel, or hair conditioner.

7. The cosmetic product according to claim 5, characterized in that, The cosmetic product contains 0.1%-10% of the composition.

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