Biochemically modified antibacterial non-woven fabric and preparation method thereof

Abstract

The invention relates to the technical field of non-woven fabric preparation, in particular to biochemical modified antibacterial non-woven fabric and a preparation method thereof.After nano-zinc oxide and nano-titanium dioxide are modified through a silane coupling agent KH-570, the dispersity of nano-particles can be improved, and the antibacterial property of the non-woven fabric is improved; under illumination or contact conditions, active oxygen is released to destroy bacterial cell membranes and inhibit metabolism of the bacterial cell membranes, so that the antibacterial modified polypropylene master batch has stable antibacterial property; loading is stabilized through a polydopamine coating, so that the antibacterial performance of the lysozyme is stabilized, the lysozyme can specifically hydrolyze a peptidoglycan layer of a bacterial cell wall to cause bacterial lysis, thiourea dioxide serving as a reducing agent can maintain the activity of the lysozyme, oxidative inactivation is avoided, meanwhile, the activity of bacterial metabolic enzymes is inhibited, and the antibacterial activity of the lysozyme is improved. Through the inorganic / biological double-effect synergistic effect, gram-positive bacteria, gram-negative bacteria and fungi can be covered, so that the antibacterial performance of the non-woven fabric is improved to a certain extent.

Description

Technical Field

[0001] The present invention relates to the technical field of non-woven fabric preparation, and particularly relates to a biochemically modified antibacterial non-woven fabric and a preparation method thereof. Background Art

[0002] As an efficient and low-cost non-woven material, non-woven fabrics are widely used in medical protection, hygiene products and filtration fields. However, the antibacterial properties of traditional non-woven fabrics mainly rely on physical barriers or post-finishing coatings, and there are the following technical bottlenecks: First, the antibacterial activity is single and prone to cause drug resistance. For example, silver-based antibacterial agents only kill bacteria by releasing metal ions, and long-term use may lead to enhanced microbial drug resistance; while organic antibacterial agents such as quaternary ammonium salts are easily decomposed by environmental pH and temperature, resulting in insufficient antibacterial persistence; Second, the chemical modification process is complex and has high ecological risks. For example, when introducing antibacterial components by blending or surface grafting in the prior art, high temperature and high pressure or toxic solvents are often required, resulting in nanoparticle aggregation and deterioration of fiber mechanical properties, and residual solvents may cause biological toxicity; Third, the loading efficiency of bioactive components is low. If biological molecules such as lysozyme are directly used to treat non-woven fabrics, due to the inertness of the fiber surface, the enzyme loading amount is low and it is easy to fall off, making it difficult to achieve long-term antibacterial effects.

[0003] In response to the above problems, in recent years, those skilled in the art have tried to combine inorganic antibacterial agents with bioactive substances, but there are still many defects in the technology integration. For example, the synergistic mechanism between nano-zinc oxide / titanium dioxide and lysozyme is not clear, and their dispersion stability in the fiber matrix is poor; although polydopamine can be used to improve the surface adhesion of materials, its film-forming uniformity and bio-molecule immobilization efficiency on hydrophobic substrates still need to be optimized. Therefore, how to modify the above technical defects has become a technical problem that those skilled in the art urgently need to solve. Summary of the Invention

[0004] Technical Problem to be Solved In view of the above-mentioned disadvantages of the prior art, the present invention provides a biochemically modified antibacterial non-woven fabric and a preparation method thereof, which can effectively solve the problem that the antibacterial performance of the non-woven fabric in the prior art is not durable and stable enough.

[0005] Technical Solution To achieve the above object, the present invention is realized through the following technical solutions: A preparation method of a biochemically modified antibacterial non-woven fabric, the preparation method of the biochemically modified antibacterial non-woven fabric is as follows: S1. Using an antibacterial modified polypropylene masterbatch as a raw material, melt-blown to obtain a polypropylene non-woven fabric; S2. Subject the polypropylene non-woven fabric to alkali treatment and then polydopamine treatment, and the obtained product is denoted as a pretreated non-woven fabric; S3. Immerse the pretreated non-woven fabric in the lysozyme solution, rinse with pure water and dry it after impregnation at 37°C for 1 h. The above is recorded as one-time finishing, and the biochemically modified antibacterial non-woven fabric is obtained after 4 times of finishing.

[0006] Furthermore, the preparation steps of the antibacterial modified polypropylene masterbatch in S1 are as follows: Step 1. Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of ethanol aqueous solution, adjust the pH value to 4-5 with glacial acetic acid, add 10 parts by weight of nano-zinc oxide and 10 parts by weight of nano-titanium dioxide after stirring and dispersing, perform ultrasonic treatment and then magnetic stirring, filter and rinse 3 times with absolute ethanol, and record the product obtained after drying and grinding through a 200-mesh sieve as the inorganic antibacterial component; Step 2. Pour 80-90 parts by weight of polypropylene and 20-25 parts by weight of the inorganic antibacterial component into a high-speed mixer and mix evenly. The antibacterial modified polypropylene masterbatch is obtained after twin-screw extrusion granulation.

[0007] Furthermore, in Step 1, the volume fraction of the ethanol aqueous solution is 90%, the method of stirring and dispersing is to stir at a speed of 300-500 r / min for 30 min, the method of ultrasonic treatment is to ultrasonically disperse at a power of 200-300 W for 30 min, the method of magnetic stirring is to stir at a speed of 500-600 r / min for 2-4 h, and the method of drying treatment is to dry at a temperature of 70-80°C until constant weight.

[0008] Furthermore, in Step 2, the processing temperature of twin-screw extrusion is 170-190°C, the main screw speed is 300 r / min, and the feeding screw speed is 20 r / min.

[0009] Furthermore, the preparation method of the polypropylene non-woven fabric in S1 is as follows: Melt-blend polypropylene and the antibacterial modified polypropylene masterbatch according to a weight ratio of 19:1, and then melt-spin and prepare a polypropylene non-woven fabric through a melt-blown spinning machine. The temperature of the melt-blown spinning machine is 240-260°C, the receiving distance of the melt-blown cloth is 300 mm, the hot air pressure is 0.6 MPa, and the winding speed is 5 m / min.

[0010] Furthermore, the method of alkali treatment in S2 is as follows: Immerse the polypropylene non-woven fabric in a sodium hydroxide solution at 80°C with a mass fraction of 5% for 2 h, drain and rinse 3 times with pure water, then immerse it in an acetic acid solution with a concentration of 1 mL / L at room temperature for 10 min, rinse 3-5 times with pure water again, and dry at a temperature of 55-60°C for 2-3 h to complete the alkali treatment.

[0011] Furthermore, the method of polydopamine treatment in S2 is as follows: Weigh 20 g of dopamine hydrochloride and dissolve it in 10 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 8.5 with hydrochloric acid with a concentration of 0.1 mol / L, then immerse the alkali-treated polypropylene non-woven fabric. After oscillating in a water bath at 30 °C for 24 h, rinse it with pure water 3 - 5 times, and dry it at a temperature of 30 - 35 °C for 5 - 8 h to complete the polydopamine treatment.

[0012] Further, the preparation method of the lysozyme solution in S3 is as follows: Weigh 10 g of thiourea dioxide and dissolve it in 5 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 7.4 with hydrochloric acid with a concentration of 0.1 mol / L, then add 10 g of lysozyme and stir at a speed of 100 r / min for 5 min. The obtained solution is the lysozyme solution.

[0013] Further, the number of rinsing times for rinsing and drying with pure water in S3 is 2 - 3 times, and the drying temperature and time are 60 °C and 1 h respectively.

[0014] A biochemically modified antibacterial non-woven fabric is prepared by a preparation method of a biochemically modified antibacterial non-woven fabric.

[0015] Beneficial effects The present invention provides a biochemically modified antibacterial non-woven fabric and its preparation method. Compared with the existing well-known technologies, the present invention has the following beneficial effects: 1. After the present invention modifies nano-zinc oxide and nano-titanium dioxide with silane coupling agent KH-570, the dispersibility of nano-particles can be improved, and reactive oxygen species are released under light or contact conditions to destroy the bacterial cell membrane and inhibit its metabolism, so that the antibacterial modified polypropylene masterbatch has stable antibacterial properties; the present invention also stably loads through a polydopamine coating, thereby stabilizing the antibacterial performance of lysozyme. Lysozyme can specifically hydrolyze the peptidoglycan layer of the bacterial cell wall, resulting in bacterial lysis. As a reducing agent, thiourea dioxide can maintain the activity of lysozyme, avoid oxidation inactivation, and at the same time inhibit the activity of bacterial metabolic enzymes. Through the inorganic / biological dual-effect synergistic action, the present invention can cover Gram-positive bacteria, Gram-negative bacteria and fungi, thereby improving the antibacterial performance of the non-woven fabric to a certain extent.

[0016] 2. The present invention can increase the surface roughness of polypropylene through alkali treatment, make polydopamine firmly adhere through stacking and hydrogen bonds, form a stable adhesion layer, prevent the shedding of lysozyme, and the multiple loadings of four times of impregnation finishing can form a gradient coating, so that lysozyme molecules are embedded in the three-dimensional network structure of polydopamine, thereby enhancing the slow-release performance and maintaining the persistence of antibacterial performance; the materials in the present invention have antibacterial, anti-fouling and self-cleaning properties, and can be extended to a wider field, so as to achieve greater application value. Specific embodiments

[0017] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0018] The present invention will be further described below in conjunction with embodiments.

[0019] The sources of some components in the examples and comparative examples are as follows: Polypropylene, model Z30S, Xinjiang Dushanzi Petrochemical Co., Ltd., Sinopec; Silane coupling agent KH-570, Shandong Rongsheng New Materials Co., Ltd.; Nano zinc oxide, model MS-K003, Guangzhou Zanyu Antimildew Technology Co., Ltd.; Nano titanium dioxide, Jinan Hongquan Titanium Industry Co., Ltd.; Hydrochloric acid dopamine, Aladdin Reagent Co., Ltd.; Tris buffer solution, Sinopharm Chemical Reagent Co., Ltd.; Thiourea dioxide, Aladdin Reagent Co., Ltd.; Lysozyme, lysozyme 20000U / mg, Guangxi Pangbo Bioengineering Co., Ltd.

[0020] Example 1 The preparation method of a biochemically modified antibacterial non-woven fabric in this example is as follows: S1. Using an antibacterial modified polypropylene masterbatch as the raw material, a polypropylene non-woven fabric is obtained after meltblowing; Among them, the preparation steps of the antibacterial modified polypropylene masterbatch are as follows: Step 1. Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of an ethanol aqueous solution with a volume fraction of 90%. Adjust the pH value to 4 with glacial acetic acid. After stirring at a speed of 300 r / min for 30 min, add 10 parts by weight of nano zinc oxide and 10 parts by weight of nano titanium dioxide. After ultrasonic dispersion at a power of 200 W for 30 min, stir at a speed of 500 r / min for 2 h. After filtration, rinse with absolute ethanol 3 times, place at a temperature of 70 °C and dry to constant weight, and then grind through a 200-mesh sieve to obtain the inorganic antibacterial component; Step 2. Pour 80 parts by weight of polypropylene and 20 parts by weight of the inorganic antibacterial component into a high-speed mixer and mix evenly. After twin-screw extrusion granulation, the obtained product is the antibacterial modified polypropylene masterbatch. The processing temperature of the twin-screw extrusion is 170 °C, the main screw speed is 300 r / min, and the feeding screw speed is 20 r / min.

[0021] The preparation method of the polypropylene non-woven fabric is as follows: The polypropylene and the antibacterial modified polypropylene masterbatch are melt-blended at a weight ratio of 19:1, and then melt-blown into a polypropylene non-woven fabric by a melt-blown spinning machine. The temperature of the melt-blown spinning machine is 240 °C, the receiving distance of the melt-blown cloth is 300 mm, the hot air pressure is 0.6 MPa, and the winding speed is 5 m / min.

[0022] S2. The polypropylene non-woven fabric is subjected to alkali treatment and then polydopamine treatment, and the obtained product is denoted as the pretreated non-woven fabric; Among them, the method of alkali treatment is: The polypropylene non-woven fabric is immersed in a sodium hydroxide solution at 80 °C with a mass fraction of 5% for 2 h, drained, rinsed 3 times with pure water, then immersed in an acetic acid solution with a concentration of 1 mL / L at room temperature for 10 min, rinsed 3 times again with pure water, and then dried at a temperature of 55 °C for 2 h to complete the alkali treatment.

[0023] The method of polydopamine treatment is: Weigh 20 g of dopamine hydrochloride and dissolve it in 10 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 8.5 with hydrochloric acid with a concentration of 0.1 mol / L, then immerse the alkali-treated polypropylene non-woven fabric, oscillate in a water bath at 30 °C for 24 h, rinse 3 times with pure water, and dry at a temperature of 30 °C for 5 h to complete the polydopamine treatment.

[0024] S3. Immerse the pretreated non-woven fabric in the lysozyme solution, immerse it at a temperature of 37 °C for 1 h, then rinse and dry it with pure water. The above is recorded as one-time finishing. After finishing 4 times, the obtained product is the biochemically modified antibacterial non-woven fabric. The number of rinsing times for rinsing and drying with pure water is 2 times, and the drying temperature and time are 60 °C and 1 h respectively; Among them, the preparation method of the lysozyme solution is: Weigh 10 g of thiourea dioxide and dissolve it in 5 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 7.4 with hydrochloric acid with a concentration of 0.1 mol / L, then add 10 g of lysozyme and stir at a speed of 100 r / min for 5 min. The obtained product is the lysozyme solution.

[0025] A biochemically modified antibacterial non-woven fabric is prepared by a preparation method of a biochemically modified antibacterial non-woven fabric.

[0026] Example 2 In the preparation method of a biochemically modified antibacterial non-woven fabric of this example, the preparation method of the biochemically modified antibacterial non-woven fabric is: S1. Using the antibacterial modified polypropylene masterbatch as the raw material, a polypropylene non-woven fabric is prepared by melt-blowning; Among them, the preparation steps of the antibacterial modified polypropylene masterbatch are as follows: Step 1: Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of an ethanol aqueous solution with a volume fraction of 90%. Adjust the pH value to 5 with glacial acetic acid. After stirring at a speed of 500 r / min for 30 min, add 10 parts by weight of nano-zinc oxide and 10 parts by weight of nano-titanium dioxide. After ultrasonic dispersion at a power of 300 W for 30 min, stir at a speed of 600 r / min for 4 h. Filter and rinse 3 times with absolute ethanol. Place it at a temperature of 80 °C and dry to constant weight, then grind it through a 200-mesh sieve to obtain the inorganic antibacterial component. Step 2: Pour 90 parts by weight of polypropylene and 25 parts by weight of the inorganic antibacterial component into a high-speed mixer and mix evenly. After twin-screw extrusion granulation, the obtained product is the antibacterial modified polypropylene masterbatch. The processing temperature of twin-screw extrusion is 190 °C, the main screw speed is 300 r / min, and the feeding screw speed is 20 r / min.

[0027] The preparation method of the polypropylene non-woven fabric is as follows: Melt-blend polypropylene and the antibacterial modified polypropylene masterbatch at a weight ratio of 19:1, and then melt-spin and prepare it into a polypropylene non-woven fabric through a melt-blown spinning machine. The temperature of the melt-blown spinning machine is 260 °C, the receiving distance of the melt-blown cloth is 300 mm, the hot air pressure is 0.6 MPa, and the winding speed is 5 m / min.

[0028] S2: Perform alkali treatment on the polypropylene non-woven fabric and then perform polydopamine treatment. The obtained product is denoted as the pretreated non-woven fabric. Among them, the method of alkali treatment is as follows: Immerse the polypropylene non-woven fabric in a sodium hydroxide solution at 80 °C with a mass fraction of 5% for 2 h. Drain and rinse 3 times with pure water. Then immerse it in an acetic acid solution with a concentration of 1 mL / L at room temperature for 10 min. Rinse 5 times with pure water again and place it at a temperature of 60 °C and dry for 3 h to complete the alkali treatment.

[0029] The method of polydopamine treatment is as follows: Weigh 20 g of dopamine hydrochloride and dissolve it in 10 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 8.5 with hydrochloric acid with a concentration of 0.1 mol / L, then immerse the alkali-treated polypropylene non-woven fabric. Oscillate in a water bath at 30 °C for 24 h, then rinse 5 times with pure water. Place it at a temperature of 35 °C and dry for 8 h to complete the polydopamine treatment.

[0030] S3. Immerse the pretreated non-woven fabric in the lysozyme solution, rinse it with pure water and dry it after impregnation at 37°C for 1 h. The above is recorded as one-time finishing. After finishing 4 times, the obtained product is the biochemically modified antibacterial non-woven fabric. The number of rinsing times with pure water and drying is 3 times, and the drying temperature and time are 60°C and 1 h respectively. Among them, the preparation method of the lysozyme solution is as follows: Weigh 10 g of thiourea dioxide and dissolve it in 5 L of Tris buffer solution with a concentration of 0.01 mol / L. After adjusting the pH value to 7.4 with hydrochloric acid with a concentration of 0.1 mol / L, add 10 g of lysozyme and stir at a speed of 100 r / min for 5 min. The obtained product is the lysozyme solution.

[0031] A biochemically modified antibacterial non-woven fabric is prepared by a preparation method of a biochemically modified antibacterial non-woven fabric.

[0032] Example 3 The preparation method of a biochemically modified antibacterial non-woven fabric in this example. The preparation method of the biochemically modified antibacterial non-woven fabric is as follows: S1. Use the antibacterial modified polypropylene masterbatch as the raw material, and obtain the polypropylene non-woven fabric after melt blowing. Among them, the preparation steps of the antibacterial modified polypropylene masterbatch are as follows: Step 1. Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of ethanol aqueous solution with a volume fraction of 90%. Adjust the pH value to 5 with glacial acetic acid, stir at a speed of 400 r / min for 30 min, then add 10 parts by weight of nano-zinc oxide and 10 parts by weight of nano-titanium dioxide, ultrasonically disperse at a power of 300 W for 30 min, then stir at a speed of 600 r / min for 3 h, filter, rinse 3 times with absolute ethanol, dry at 75°C to constant weight, and grind through a 200-mesh sieve. The obtained product is recorded as the inorganic antibacterial component. Step 2. Pour 85 parts by weight of polypropylene and 23 parts by weight of the inorganic antibacterial component into a high-speed mixer and mix evenly. After twin-screw extrusion granulation, the obtained product is the antibacterial modified polypropylene masterbatch. The processing temperature of twin-screw extrusion is 180°C, the main screw speed is 300 r / min, and the feeding screw speed is 20 r / min.

[0033] The preparation method of the polypropylene non-woven fabric is as follows: Melt-blend polypropylene and the antibacterial modified polypropylene masterbatch according to a weight ratio of 19:1, and then prepare the polypropylene non-woven fabric by melt blowing with a melt-blown spinning machine. The temperature of the melt-blown spinning machine is 250°C, the receiving distance of the melt-blown cloth is 300 mm, the hot air pressure is 0.6 MPa, and the winding speed is 5 m / min.

[0034] S2. Perform alkali treatment on the polypropylene non-woven fabric and then perform polydopamine treatment. The obtained product is recorded as the pretreated non-woven fabric. Among them, the method of alkali treatment is as follows: Immerse the polypropylene non-woven fabric in a sodium hydroxide solution at 80 °C with a mass fraction of 5% for 2 h, drain it, rinse it 3 times with pure water, then immerse it in an acetic acid solution with a concentration of 1 mL / L at room temperature for 10 min, rinse it 4 times again with pure water, and then dry it at a temperature of 58 °C for 3 h to complete the alkali treatment.

[0035] The method of polydopamine treatment is as follows: Weigh 20 g of dopamine hydrochloride and dissolve it in 10 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 8.5 with hydrochloric acid with a concentration of 0.1 mol / L, then immerse the alkali-treated polypropylene non-woven fabric in it, oscillate it in a water bath at 30 °C for 24 h, rinse it 4 times with pure water, and dry it at a temperature of 33 °C for 7 h to complete the polydopamine treatment.

[0036] S3. Immerse the pretreated non-woven fabric in the lysozyme solution, immerse it at a temperature of 37 °C for 1 h, then rinse it with pure water and dry it. The above is recorded as one finishing time. After finishing 4 times, the obtained product is the biochemically modified antibacterial non-woven fabric. The number of rinsing times for rinsing with pure water and drying is 3 times, and the drying temperature and time are 60 °C and 1 h respectively; Among them, the preparation method of the lysozyme solution is as follows: Weigh 10 g of thiourea dioxide and dissolve it in 5 L of Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 7.4 with hydrochloric acid with a concentration of 0.1 mol / L, then add 10 g of lysozyme and stir it at a speed of 100 r / min for 5 min. The obtained product is the lysozyme solution.

[0037] A biochemically modified antibacterial non-woven fabric is prepared by a preparation method of a biochemically modified antibacterial non-woven fabric.

[0038] Comparative Example 1 A biochemically modified antibacterial non-woven fabric and its preparation method provided in this comparative example are generally the same as those in Example 1. The main difference is that in this Comparative Example 1, the antibacterial modified polypropylene masterbatch in Example 1 is replaced with a polypropylene masterbatch.

[0039] Comparative Example 2 A biochemically modified antibacterial non-woven fabric and its preparation method provided in this comparative example are generally the same as those in Example 1. The main difference is that in this Comparative Example 2, the alkali treatment in Example 1 is not carried out.

[0040] Comparative Example 3 A biochemically modified antibacterial non-woven fabric and its preparation method provided in this comparative example are generally the same as those in Example 1. The main difference is that in this Comparative Example 3, the polydopamine treatment in Example 1 is not carried out.

[0041] Performance Test The biochemically modified antibacterial non-woven fabrics prepared in Examples 1-3 and Comparative Examples 1-3 were respectively labeled as Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3. Then, referring to the standard of GB / T20944.3-2008, the antibacterial properties of Examples 1-3 and Comparative Examples 1-3 were detected. The tested bacterial strains were Escherichia coli, Pseudomonas, Bacillus and Staphylococcus aureus. The obtained data were recorded in the following table: It can be seen from the data in the above table that the biochemically modified antibacterial non-woven fabrics prepared in Examples 1-3 of the present invention have a higher antibacterial rate, indicating that using the antibacterial modified polypropylene masterbatch in the present invention as the basic raw material can achieve better antibacterial performance during the preparation process of the biochemically modified antibacterial non-woven fabric. It also shows that the non-woven fabric can achieve better antibacterial effect after being treated with alkali, polydopamine and then immersed in lysozyme solution.

[0042] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the phrase "including a..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0043] The above examples are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing examples, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing examples, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various examples of the present invention.

Claims

1. A preparation method of a biochemically modified antibacterial non-woven fabric, characterized in that, The preparation method of the biochemically modified antibacterial non-woven fabric is as follows: S1. Using an antibacterial modified polypropylene masterbatch as the raw material, a polypropylene non-woven fabric is prepared by melt blowing. S2. The polypropylene non-woven fabric is subjected to alkali treatment and then polydopamine treatment, and the obtained product is denoted as the pretreated non-woven fabric. S3. The pretreated non-woven fabric is immersed in a lysozyme solution, impregnated at a temperature of 37 °C for 1 h, rinsed with pure water and dried. The above is recorded as one finishing treatment. After four finishing treatments, the obtained product is the biochemically modified antibacterial non-woven fabric.

2. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 1, characterized in that, The preparation steps of the antibacterial modified polypropylene masterbatch in S1 are as follows: Step 1. Dissolve 2 parts by weight of silane coupling agent KH-570 in 500 parts by weight of an ethanol aqueous solution, adjust the pH value to 4-5 with glacial acetic acid, add 10 parts by weight of nano-zinc oxide and 10 parts by weight of nano-titanium dioxide after stirring and dispersing, perform ultrasonic treatment and then magnetic stirring, filter, rinse 3 times with absolute ethanol, and after drying treatment, grind through a 200-mesh sieve to obtain the inorganic antibacterial component. Step 2. Pour 80-90 parts by weight of polypropylene and 20-25 parts by weight of the inorganic antibacterial component into a high-speed mixer and mix evenly. After twin-screw extrusion granulation, the obtained product is the antibacterial modified polypropylene masterbatch.

3. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 2, characterized in that, In Step 1, the volume fraction of the ethanol aqueous solution is 90%. The method of stirring and dispersing is to stir at a speed of 300-500 r / min for 30 min. The method of ultrasonic treatment is to ultrasonically disperse at a power of 200-300 W for 30 min. The method of magnetic stirring is to stir at a speed of 500-600 r / min for 2-4 h. The method of drying treatment is to dry at a temperature of 70-80 °C until constant weight.

4. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 2, characterized in that, In Step 2, the processing temperature of twin-screw extrusion is 170-190 °C, the main screw speed is 300 r / min, and the feeding screw speed is 20 r / min.

5. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 1, characterized in that, The preparation method of the polypropylene non-woven fabric in S1 is as follows: Polypropylene and the antibacterial modified polypropylene masterbatch are melt-blended at a weight ratio of 19:1, and then melt-blown into a polypropylene non-woven fabric by a melt-blown spinning machine. The temperature of the melt-blown spinning machine is 240-260 °C, the receiving distance of the melt-blown cloth is 300 mm, the hot air pressure is 0.6 MPa, and the winding speed is 5 m / min.

6. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 1, characterized in that, The method of alkali treatment in S2 is as follows: Immerse the polypropylene non-woven fabric in a sodium hydroxide solution at 80 °C with a mass fraction of 5% for 2 h, drain, rinse 3 times with pure water, then immerse in an acetic acid solution with a concentration of 1 mL / L at room temperature for 10 min, rinse 3-5 times again with pure water, and then dry at a temperature of 55-60 °C for 2-3 h to complete the alkali treatment.

7. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 1, characterized in that, The method of polydopamine treatment in S2 is as follows: Weigh 20 g of dopamine hydrochloride and dissolve it in 10 L of a Tris buffer solution with a concentration of 0.01 mol / L. Adjust the pH value to 8.5 with 0.1 mol / L hydrochloric acid, then immerse the alkali-treated polypropylene non-woven fabric, oscillate in a water bath at 30 °C for 24 h, rinse 3-5 times with pure water, and dry at a temperature of 30-35 °C for 5-8 h to complete the polydopamine treatment.

8. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 1, characterized in that, The preparation method of the lysozyme solution in S3 is as follows: Weigh 10 g of thiourea dioxide and dissolve it in 5 L of Tris buffer with a concentration of 0.01 mol / L. After adjusting the pH value to 7.4 with hydrochloric acid with a concentration of 0.1 mol / L, add 10 g of lysozyme and stir at a speed of 100 r / min for 5 min. The resulting solution is the lysozyme solution.

9. The preparation method of a biochemically modified antibacterial non-woven fabric according to claim 1, characterized in that, In S3, the number of rinsing times with pure water and drying is 2 - 3 times, and the drying temperature and time are 60 °C and 1 h respectively.

10. A biochemically modified antibacterial non-woven fabric, characterized in that, It is obtained by the preparation method of a biochemically modified antibacterial non-woven fabric according to any one of claims 1 - 9.

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