Biosensor for detecting Alzheimer's disease markers and its construction method and application
Through the dual-mode ratio biosensor that combines magnetic bead separation and ruthenium silicon nanoparticles, the sensitivity and accuracy of the detection of Aβ oligomers of Alzheimer's disease marker is solved, and efficient detection of Aβ oligomers is achieved.
CN120405152BActive Publication Date: 2025-09-02XUZHOU CENT HOSPITAL
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Patent Information
- Application Number
- CN202510913252.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-03
- Publication Date
- 2025-09-02
- Estimated Expiration
- 2045-07-03
AI Technical Summary
Technical Problem
The prior art is difficult to achieve high sensitivity and accurate detection of Alzheimer's disease marker Aβ oligomers, especially in complex biological samples.
Method used
Using magnetic bead separation technology and aptamers, ruthenium silicon nanoparticles (RuSi NPs) are used as electrochemiluminescence active substrates and ferrocene is used as quencher. The hybrid chain reaction is activated by entropy-driven DNA cycle to perform signal amplification, and a dual-mode ratio biosensor is constructed.
Benefits of technology
High sensitivity and accurate detection of Alzheimer's disease marker Aβ oligomers is achieved, which reduces background signal interference and improves the selectivity and accuracy of detection.
✦ Generated by Eureka AI based on patent content.
Abstract
The present invention discloses a biosensor for detecting Alzheimer's disease markers, as well as a construction method and application thereof, relating to the field of biosensor technology. The construction method comprises the following steps: after cleaning a glassy carbon electrode, dripping a RuSi NPs@CS complex onto the surface of the cleaned glassy carbon electrode to obtain a RuSi NPs@CS / GCE electrode; immersing the RuSi NPs@CS / GCE electrode in a PHL:S double-strand solution for incubation to obtain the biosensor; the RuSi NPs@CS complex is obtained by mixing ruthenium silicon nanoparticles and chitosan; the PHL:S double-strand solution contains PHL chains activated by carboxyl groups and S chains through an incubation reaction. The biosensor has the advantages of high accuracy, strong sensitivity, and good selectivity, and can be used to accurately detect Aβ oligomers, a marker of Alzheimer's disease.
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