Plant extract with antibacterial, antioxidant and antipruritic triple effects, composition, preparation method and application thereof

Abstract

The present invention provides a plant extract, composition, preparation method, and application thereof, exhibiting triple antibacterial, antioxidant, and antipruritic effects. These extracts relate to the technical field of daily chemical products. The plant extract comprises a and b; a comprises Terminalia chebula fruit extract; b is a plum fruit extract, or at least one of a rose flower extract, a rose flower extract, or a tribulus terrestris fruit extract and a plum fruit extract; when b is a plum fruit extract, the weight ratio of a to b is 2-4:1; when b is at least one of a rose flower extract, a rose flower extract, or a tribulus terrestris fruit extract and a plum fruit extract, the weight ratio of a to b is 1:1. The plant extract exhibits excellent antibacterial, antioxidant, and antipruritic effects, achieving a strong synergistic effect, thereby achieving enhanced results when applied to skin care products. This invention addresses the technical problem of the prior art lacking a plant extract that simultaneously exhibits triple antibacterial, antioxidant, and antipruritic effects.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemical products, and in particular to a plant extract having the triple effects of antibacterial, anti-oxidation and antipruritic, a composition, and a preparation method and application thereof. Background Art

[0002] Dandruff, commonly known as pityriasis capitis, causes itching, flaking, and mild inflammation. Dandruff is closely linked to a dysfunction of the scalp's barrier function. The scalp barrier can be divided into three main functions: permeability, antimicrobial, antioxidant, immune response, and photoprotection. Abnormalities in these functional barriers disrupt the normal physiological function of the scalp. If not promptly repaired, scalp problems or diseases may develop. The antimicrobial and antioxidant barriers, in particular, play a key role in resisting pathogen invasion and reducing scalp cell damage (slowing scalp aging). Therefore, these two barriers are particularly crucial in the prevention and treatment of dandruff. Furthermore, Malassezia fungi secrete lipases that break down triglycerides and saturated fatty acids in sebum into unsaturated fatty acids, disrupting the stratum corneum barrier and causing inflammation and itching. Furthermore, acne, athlete's foot, tinea, dermatitis, and psoriasis are all associated with impaired antimicrobial and antioxidant barriers, as well as itching.

[0003] Commonly used chemical anti-dandruff agents on the market include salicylic acid, selenium disulfide, zinc pyrithione (ZPT), piroctone olamine (OCT), climbazole, and climbazole. Salicylic acid is a keratin exfoliant that lacks significant antifungal efficacy, has poor anti-dandruff effects, is highly irritating, and can easily exfoliate the skin and cause damage. Selenium disulfide is a cell growth inhibitor and is highly irritating. It can cause dryness after use, and long-term use can cause hair loss and odor. Zinc pyrithione (ZPT, banned in the EU), piroctone olamine (OCT), climbazole, and ketoconazole are all powerful fungicides that are highly irritating and environmentally unfriendly. Long-term use of these chemical agents can lead to drug resistance and damage the immune system.

[0004] In recent years, plant-based treatments for dandruff, acne, and other skin conditions have attracted significant attention due to their minimal side effects and low drug resistance. However, currently used plant extracts have limited efficacy and are unable to meet the complex and ever-changing needs of the scalp.

[0005] In view of this, the present invention is proposed. Summary of the Invention

[0006] One of the purposes of the present invention is to provide a plant extract with the triple effects of antibacterial, antioxidant and antipruritic, so as to solve the technical problem in the prior art that there is a lack of plant extracts that can simultaneously have the triple effects of antibacterial, antioxidant and antipruritic.

[0007] A second object of the present invention is to provide a method for preparing the above-mentioned plant extract.

[0008] The third object of the present invention is to provide a composition having the triple effects of antibacterial, antioxidant and antipruritic.

[0009] A fourth object of the present invention is to provide a method for preparing the above-mentioned composition.

[0010] A fifth object of the present invention is to provide use of the above-mentioned plant extract, the plant extract obtained by the above-mentioned preparation method, the above-mentioned composition, or the composition obtained by the above-mentioned preparation method in the preparation of scalp care products or hair care products.

[0011] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are adopted:

[0012] In a first aspect, the present invention provides a plant extract having the triple effects of antibacterial, antioxidant and antipruritic, wherein the plant extract comprises a and b;

[0013] aIncluding Terminalia chebula fruit extract;

[0014] b is plum fruit extract, or at least one of rose flower extract, rose flower extract or tribulus terrestris fruit extract and plum fruit extract;

[0015] When b is plum fruit extract, the weight ratio of a to b is 2-4:1;

[0016] When b is at least one of rose flower extract, rose flower extract or tribulus terrestris fruit extract and plum fruit extract, the weight ratio of a to b is 1:1.

[0017] Furthermore, the b is plum fruit extract.

[0018] Furthermore, said b is plum fruit extract and flower extract;

[0019] The weight ratio of the plum fruit extract to the flower extract is 1:1; the flower extract is rose flower extract or rose flower extract.

[0020] Furthermore, the b is plum fruit extract, flower extract and tribulus terrestris fruit extract;

[0021] The weight ratio of the plum fruit extract, the flower extract and the tribulus terrestris fruit extract is 10:7-9:1-3; the flower extract includes rose flower extract or rose flower extract.

[0022] In a second aspect, the present invention provides a method for preparing the above-mentioned plant extract, comprising mixing and soaking the plant raw materials of each component with the extractant in a volume ratio of 1:8 to 12, ultrasonicating, concentrating the filtrate, centrifuging and obtaining the supernatant to obtain a single plant extract, and mixing according to a formula to obtain the plant extract;

[0023] Preferably, the extractant comprises 50% to 80% ethanol, 30% to 80% butanediol or water;

[0024] Preferably, the ultrasound duration is 30 to 90 minutes;

[0025] Preferably, the soaking time is 0.5 to 2.5 hours.

[0026] In a third aspect, the present invention provides a composition having the triple effects of antibacterial, antioxidant and antipruritic, comprising the above-mentioned plant extract or the plant extract obtained by the above-mentioned preparation method, and cypress phenol, wherein the weight ratio of the plant extract to cypress phenol is 1~30:1.

[0027] Furthermore, the weight ratio of the plant extract to hinokitiol is 1 to 3:1.

[0028] In a fourth aspect, the present invention provides a method for preparing the above-mentioned composition, comprising mixing a hinokitiol solution with a plant extract according to a formula.

[0029] Furthermore, the concentration of hinokitiol in the hinokitiol alcohol solution is 1-200 mg / mL, preferably 50 mg / mL.

[0030] In a fifth aspect, the present invention provides the use of the aforementioned plant extract, the plant extract obtained by the aforementioned preparation method, the aforementioned composition, or the composition obtained by the aforementioned preparation method in the preparation of scalp care products or hair care products.

[0031] The present invention provides a plant extract with triple antibacterial, antioxidant and antipruritic effects. When the terminalia chebula fruit extract is compounded with plum fruit extract, rose flower extract, rose flower extract or tribulus terrestris fruit extract within a certain weight ratio range, they can cooperate with each other to exert good antibacterial, antioxidant and antipruritic effects, achieve a strong synergistic effect, and thus achieve better effects when applied to skin care products. This solves the technical problem that there is a lack of plant extracts that can simultaneously have the triple effects of antibacterial, antioxidant and antipruritic effects in the prior art. On the other hand, a composition with triple antibacterial, antioxidant and antipruritic effects is provided. When the above-mentioned plant extracts are compounded with cypress phenol in a certain weight ratio, each component acts on skin problems, especially scalp problems, through multiple targets and multiple dimensions, which can significantly enhance the antibacterial, antioxidant and antipruritic effects of the composition, thereby protecting the overall health of the skin. And under a limited ratio, it shows better antibacterial, antioxidant and antipruritic effects than the chemical antidandruff agent OCT and cypress phenol used alone. DETAILED DESCRIPTION

[0032] Unless otherwise defined herein, scientific and technical terms used in conjunction with the present invention shall have the meanings commonly understood by those of ordinary skill in the art. The meaning and scope of the terms should be clear; however, in the event of any potential ambiguity, the definitions provided herein take precedence over any dictionary or external definitions. In this application, the use of "or" means "and / or" unless otherwise stated. In addition, the use of the term "including" and other forms is non-limiting.

[0033] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] In one aspect, the present invention provides a plant extract having the triple effects of antibacterial, antioxidant and antipruritic, wherein the plant extract comprises a and b;

[0035] a includes Terminalia chebula fruit extract; b is plum fruit extract, or at least one of rose flower extract, rose flower extract or tribulus terrestris fruit extract and plum fruit extract;

[0036] When b is plum fruit extract, the weight ratio of a to b is 2-4:1;

[0037] When b is at least one of rose flower extract, rose flower extract or tribulus terrestris fruit extract and plum fruit extract, the weight ratio of a to b is 1:1.

[0038] When combined with plum fruit extract, rose flower extract, Chinese rose flower extract, or tribulus terrestris fruit extract within a certain weight ratio, the compound can exert excellent antibacterial, antioxidant, and antipruritic effects, achieving a strong synergistic effect, thereby achieving better results when used in skin care products. This solves the technical problem of the lack of plant extracts that simultaneously possess antibacterial, antioxidant, and antipruritic effects in the prior art.

[0039] In some specific embodiments, the b is plum fruit extract.

[0040] In some specific embodiments, the b is a plum fruit extract and a flower extract, and the weight ratio of the plum fruit extract to the flower extract is 1: 1. In some specific embodiments, the flower extract is a rose flower extract or a rose flower extract.

[0041] In some specific embodiments, the b is plum fruit extract, flower extract and tribulus terrestris fruit extract, and the weight ratio of the plum fruit extract, flower extract and tribulus terrestris fruit extract is 10:7-9:1-3.

[0042] The weight ratio of the plum fruit extract, the flower extract and the tribulus terrestris fruit extract can be, but is not limited to, 10:7:1, 10:7:2, 10:7:3, 10:8:1, 10:8:2, 10:8:3, 10:9:1 or 10:9:3, or any value between 10:7 and 9:1 and 3.

[0043] In some specific embodiments, the flower extract is a rose extract or a rose extract.

[0044] According to another aspect of the present invention, a method for preparing the above-mentioned plant extract is also provided, comprising mixing and soaking the plant raw materials of each component with the extractant in a volume ratio of 1:8 to 12, ultrasonicating, concentrating the filtrate, centrifuging and obtaining the supernatant to obtain a single plant extract, and mixing according to a formula to obtain the plant extract.

[0045] In some specific embodiments, the extractant includes 50% to 80% ethanol, 30% to 80% butanediol or water.

[0046] The concentration of ethanol may be, but is not limited to, 50%, 55%, 60%, 65%, 70%, 75% or 80%, or any value between 50% and 80%.

[0047] The concentration of the butanediol may be, but is not limited to, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75% or 80%, or any value between 30% and 80%.

[0048] In some specific embodiments, the ultrasonication time is 30 to 90 minutes; in some specific embodiments, the soaking time is 0.5 to 2.5 hours.

[0049] According to another aspect of the present invention, a composition having the triple effects of antibacterial, antioxidant and antipruritic is provided, comprising the above-mentioned plant extract or the plant extract obtained by the above-mentioned preparation method, and cypress phenol, wherein the weight ratio of the plant extract to cypress phenol is 1~30:1.

[0050] When these plant extracts are combined with hinokitiol in a specific weight ratio, each component acts on skin problems, particularly scalp problems, through multiple targets and dimensions, significantly enhancing the antibacterial, antioxidant, and antipruritic properties of the composition, thereby protecting overall skin health. Furthermore, at this defined ratio, the combination demonstrates superior antibacterial, antioxidant, and antipruritic effects compared to the chemical antidandruff agent OCT and hinokitiol alone.

[0051] The weight ratio of the plant extract to hinokitiol can be, but is not limited to, 1:1, 1.3:1, 1.5:1, 1.8:1, 2:1, 2.3:1, 2.5:1, 2.8:1, 3:1, 5:1, 10:1, 15:1, 20:1, 25:1 or 30:1, or any value between 1 and 30:1, preferably 1 to 3:1.

[0052] According to another aspect of the present invention, a method for preparing the above-mentioned composition is also provided, comprising mixing the hinokitiol solution with the plant extract according to a formula.

[0053] In some specific embodiments, the concentration of hinokitiol in the hinokitiol alcohol solution in the above preparation method is 1-200 mg / mL, preferably 50 mg / mL.

[0054] According to another aspect of the present invention, there is also provided the use of the above-mentioned plant extract, the plant extract prepared by the above-mentioned preparation method, the above-mentioned composition or the composition prepared by the above-mentioned preparation method in preparing scalp care products or hair care products.

[0055] Specifically, the product has at least one of antibacterial, antipruritic or anti-oxidant functions.

[0056] The present invention is further described below by way of examples. Unless otherwise specified, the materials in the examples were prepared according to existing methods or directly purchased from the market.

[0057] Example 1 Plant extract with triple effects of antibacterial, antipruritic and antioxidant

[0058] According to the components of the plant extract, plant extracts with different compounding components are provided, and the weight ratios of the specific compounding components are shown in Table 1.

[0059] Table 1 Proportions of plant extract compound components

[0060]

[0061] The above plant extracts are specifically prepared according to the following steps:

[0062] (1) Weigh 15 g of each component's plant medicinal material, add 10 times the amount of 70% ethanol, soak for 1 h, ultrasonically extract twice, each time for 20 min, combine the two filtrates and concentrate to 1 g / mL, centrifuge to obtain the plant supernatant of each component, which is a single plant extract, and store at 4 °C for later use;

[0063] (2) The total concentration of the plant supernatant obtained above was diluted to 200 mg / mL. Without changing the total concentration, the proportions of the components were adjusted according to Table 1 to prepare a plant extract.

[0064] Example 2 A composition with triple effects of antibacterial, antipruritic and antioxidant

[0065] 1. Composition consisting of plant extracts and hinokitiol

[0066] According to the components of the composition, a composition with different components is provided, and the weight ratio of the composition formula is shown in Table 2.

[0067] Table 2 Ratio of plant extracts and hinokitiol compound components

[0068]

[0069] The compositions in Table 2 were prepared according to the following steps:

[0070] (1) Dissolve hinokitiol in ethanol or butanediol (with 0.2% Tween as solubilizer) to prepare a 50 mg / mL hinokitiol alcohol solution as a hinokitiol stock solution; seal the tube of the stock solution with parafilm and store in a refrigerator at 4°C away from light until ready for use;

[0071] (2) The plant extract and the cypress phenol stock solution were mixed so that the total concentration of the plant extract and cypress phenol synergistic test formula was 50 mg / mL. Without changing the total concentration, the proportions of the components were adjusted according to Table 2 to prepare the composition shown in Table 2.

[0072] Experiment 1: Antibacterial effect test

[0073] In this test, the minimum inhibitory concentration (MIC) of a single plant extract, cypermethrin, each plant extract prepared in Example 1, each composition prepared in Example 2, and OCT was determined by the broth microdilution method to evaluate the inhibitory effect of each test drug on Malassezia; the synergistic antibacterial effect of each composition was evaluated by exploring the synergistic effect index (CI), and the specific method is as follows.

[0074] 1. Test materials

[0075] Malassezia furfur (M. furfur, ATCC44344) was purchased from Shanghai Microorganism Collection Center, piroctone olamine (OCT) was purchased from Tongmeiyuan (Guangdong) Biotechnology Co., Ltd., and Terminalia chebula fruit, plum fruit, rose flower, Chinese rose flower, and Tribulus terrestris fruit were purchased from Guangzhou Renshen Pharmaceutical Chain Co., Ltd.

[0076] 2. Test Method

[0077] (1) Preparation of modified Dxion (mDixon) medium and modified Dxion (mDixon) agar medium: According to the instructions, accurately weigh the medium granules, agar powder and deionized water and place them in a beaker. Stir with a glass rod until the granules are completely dissolved. Adjust the pH value to 6.1±0.1, dispense into conical flasks, seal them, and sterilize them by high pressure at 121℃ for 30min. After cooling, store them in a refrigerator at 4℃ for later use.

[0078] (2) Preparation of bacterial suspension: Take out the glycerol tube of the test bacteria stored in the -20℃ refrigerator, use an inoculation loop to take 20μL of bacterial solution, inoculate it on the agar slant by streak method, place it in a constant temperature incubator at 32℃ and culture it for 2 days, rinse the slant with physiological saline to obtain a high-concentration bacterial suspension; take another test tube, pour in an appropriate amount of physiological saline, add an appropriate amount of high-concentration bacterial suspension to make its turbidity consistent with that of the McFarland turbidimetric tube, and obtain a final concentration of 1×10 6 CFU / mL bacterial suspension;

[0079] (3) Determination of MIC value:

[0080] ① Culture method: add 100 μL of modified Dixon (mDixon) liquid medium to the 2nd to 11th columns of the 96-well plate, add 200 μL of modified Dixon (mDixon) liquid medium to the 12th column, add 200 μL of each test drug to the 1st column, take 100 μL and dilute it horizontally to the 10th column (discard the excess 100 μL of drug solution), then take the bacterial suspension prepared in step (2) and add it to the 1st to 11th columns, 100 μL per well; at this time, the final volume of each well is 200 μL, the 11th column is the growth control well, and the 12th column is the blank control well. Place the 96-well plate at 32°C and incubate for 1 to 2 days;

[0081] ②Evaluation criteria: The concentration of the drug solution in the sterile growth hole is the minimum inhibitory concentration (MIC) when observed with the naked eye;

[0082] (4) Synergistic antibacterial test and evaluation:

[0083] ① The Chou-Talalay model is a commonly used pharmacological model for evaluating the synergistic and antagonistic effects of compound combinations during the onset of action. The MIC of each example was measured according to the method described in (3), and the synergistic effect index (CI) was calculated using the Chou-Talalay formula to determine whether there was synergy. The formula is as follows:

[0084] ;

[0085] ②Evaluation criteria: C a 、C b 、C c 、C d are the concentrations corresponding to the antibacterial effects of components A, B, C, and D when acting alone; C A 、C B 、C C 、C D It indicates the inhibitory effect observed at the corresponding concentration when each component is compounded into a composition, C A-D Result = Composition MIC experimental data × C A-D The proportion of the component in the composition; CI < 1, it means there is a synergistic effect; CI = 1, it means there is no interaction; CI > 1, it means there is an antagonistic effect.

[0086] 3. Test results

[0087] (1) Table 3 shows the antibacterial results of single drugs.

[0088] Table 3 Single drug antibacterial results

[0089]

[0090] As shown in Table 3, hinokitiol has excellent antibacterial effect, which is significantly better than the chemical antidandruff agent OCT, and its MIC is 1 / 2 of OCT; plant extracts have good antibacterial effect, and their MICs are all less than 50 mg / mL, but none of them are equivalent to OCT.

[0091] (2) Tables 4 and 5 show the results of the synergistic antibacterial test of the plant extracts of Example 1.

[0092] Table 4 Plant extract synergistic test results 1 (mg / mL)

[0093]

[0094] As shown in Table 4, when the weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract was 2:1 to 4:1, the CI was less than 1, indicating a synergistic antibacterial effect, with the MIC being 0.78125 mg / mL. Multiple plant extracts were compounded at a weight ratio of Terminalia chebula fruit extract to Prunus mume fruit extract of 2:1.

[0095] Table 5 Plant extract synergistic test results 2 (mg / mL)

[0096]

[0097] As can be seen from Table 5, plant extracts 5 to 10 show that as the content of rose extract increases, the MIC of the composition becomes smaller and the synergistic index CI becomes smaller, gradually changing from an antagonistic effect to a synergistic effect. The weight ratio of the plant extracts Terminalia chebula fruit extract and Prunus mume fruit extract is 2:1, and the weight ratio of Terminalia chebula fruit extract, Prunus mume fruit extract, rose flower extract and Tribulus terrestris fruit extract is 20:10:7-9:1-3, CI <1, and has a synergistic antibacterial effect.

[0098] Plant extracts 11 to 17 showed that as the content of rose extract increased, the MIC of the composition became smaller and the synergistic index CI became smaller, gradually changing from antagonistic effect to synergistic effect. Plant extract 17 had the strongest synergistic effect. When the weight ratio of the plant extracts Terminalia chebula fruit extract and Prunus mume fruit extract was 2:1, and the weight ratio of Terminalia chebula fruit extract, Prunus mume fruit extract, rose extract and Tribulus terrestris fruit extract was 20:10:7~9:1-3, CI <1, indicating a synergistic antibacterial effect.

[0099] Combined with the above results, it can be seen that when the weight ratio of the plant extracts Terminalia chebula fruit extract and Prunus mume fruit extract is 2:1, and the weight ratio of Terminalia chebula fruit extract, Prunus mume fruit extract, flower extract (rose extract or rose flower extract) and Tribulus terrestris fruit extract is 20:10:7~9:1-3, CI is <1, which has a synergistic antibacterial effect.

[0100] Plant extracts 9, 10, and 17 demonstrated superior antibacterial effects compared to the corresponding single plant extracts listed in Table 3. Rose extract also demonstrated a superior synergistic antibacterial effect compared to rose extract. Additional testing and subsequent formulations using the same hinokitiol formula were conducted using a ratio of Terminalia chebula fruit extract: Prunus mume fruit extract: Rose extract: Tribulus terrestris fruit extract of 20:10:7:3 (Plant Extract 8) and 20:10:10:0 (Plant Extract 10).

[0101] (3) Table 6 shows the results of the synergistic antibacterial test of the composition of the plant extract and cypress phenol in Example 2.

[0102] Table 6 Synergistic test results of plant extracts and hinokitiol (mg / mL)

[0103]

[0104] It can be seen from Table 6 that when the ratio of plant extract 8 to cypress phenol is 1~30:1, CI is less than 1, and the composition has a synergistic antibacterial effect, and when the ratio of plant extract 8 to cypress phenol is 1~3:1, the MIC of the composition is less than the MIC of the chemical antidandruff agent OCT.

[0105] When the ratio of plant extract 10 to cypress phenol is 1-30:1, CI is less than 1, and the composition has a synergistic antibacterial effect. When the ratio of plant extract 10 to cypress phenol is 1-3:1, the MIC of the composition is less than the MIC of the chemical antidandruff agent OCT.

[0106] That is, when the ratio of the plant extract to cypress phenol is 1-30:1, it has a synergistic antibacterial effect. When the ratio of the plant extract to cypress phenol is 1-3:1, the MIC of the composition is lower than the MIC of the chemical anti-dandruff agent OCT.

[0107] Experiment 2 Determination of DPPH free radical scavenging ability

[0108] This test evaluates the ability of each raw material to resist oxidative damage by measuring its ability to scavenge DPPH free radicals.

[0109] 1. Test materials: 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0110] 2. Test Method

[0111] The samples and VC were prepared at 20 mg / ml, 2 mg / ml, 200 μg / mL, 20 μg / mL, and 2 μg / mL. 500 μL of each sample solution was pipetted into a plastic centrifuge tube. 500 μL of DPPH (0.2 mmol / L, prepared in ethanol) solution was added. After shaking and protecting from light for 30 minutes, the absorbance was measured at 517 nm using ethanol as a blank control and VC as a positive control. Three parallel experiments were performed. Based on the preliminary experimental results, the antioxidant activity of each raw material was compared at concentrations of 2 mg / ml and 200 μg / mL. The specific calculation formula is as follows:

[0112] DPPH clearance rate = (1-D1 / D2) × 100%;

[0113] Where: D1 is the absorbance of the test sample or positive control; D2 is the absorbance of the blank control.

[0114] 3. Test results

[0115] (1) Table 7 shows the test results of the scavenging ability of some raw materials in Examples 1 and 2 on DPPH free radicals.

[0116] Table 7 Test results of the scavenging ability of each raw material to DPPH free radicals

[0117]

[0118] As can be seen from Table 7, the scavenging ability of cypress phenol alone at 2 mg / ml and 200 μg / mL for DPPH free radicals is less than that of OCT, and the antioxidant activity of OCT is also relatively small; plant extract 8 has excellent antioxidant activity, and its scavenging ability for DPPH free radicals at two concentrations of 2 mg / ml and 200 μg / mL is comparable to that of VC, and is significantly different from OCT; when the plant extract is compounded with cypress phenol, the antioxidant activity of the composition can be significantly enhanced, and the scavenging ability of composition 3 for DPPH free radicals is significantly better than that of cypress phenol alone, and is also significantly different from OCT.

[0119] Therefore, the plant extract and composition provided by the present invention have significant antioxidant effects, can significantly enhance the antioxidant capacity of hinokitiol, and are significantly better than OCT.

[0120] Experiment 3: Antipruritic effect test

[0121] This test uses an itch-type mouse model to evaluate the antipruritic effect of each ingredient.

[0122] 1. Test animals and reagents:

[0123] SPF-grade male BALB / c mice, weighing 18–22 g, with animal qualification certificate number SCXK(Yue)2021-0041, were purchased from the Laboratory Animal Management Center of Southern Medical University. C48 / 80 mice were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0124] 2. Test method:

[0125] (1) Compound 48 / 80 is the pruritus in this experimental pruritus model, and the proposed subcutaneous injection dose in the back of the neck of BALB / c mice is 100 μg / mouse.

[0126] (2) Hair removal: Use a razor to remove hair from the back of each BALB / c mouse. The hair removal area is approximately 2*2 cm².

[0127] (3) Administration: BALB / c mice were numbered and subcutaneously injected with 100 μg / 50 μl of compound C48 / 80 in saline solution at the nape of the neck (50 μl). The corresponding raw materials were then applied to the depilated area on the back of the mice. The mice were observed for 20 minutes and the number of back licks within 20 minutes was recorded.

[0128] Blank control: Apply physiological saline to the depilated area on the back of the mouse and observe the number of back licking within 20 minutes.

[0129] (4) Statistical methods

[0130] The experiment used a timer and counter to simultaneously observe and record the number of BALB / c mice licking their backs within 20 minutes after drug administration in each model group. The back licking response was counted as one lick from the time the BALB / c mouse turned its head to touch the shaved area until it turned back. A lick lasting more than 1 second was counted as two licks.

[0131] 3. Test results:

[0132] (1) Table 8 shows the antipruritic test results of some raw materials in Examples 1 and 2.

[0133] Table 8 Antipruritic test results of each raw material

[0134]

[0135] As shown in Table 8, compared with OCT and hinokitiol used alone, plant extract 8 and composition 3 both have better antipruritic effects.

[0136] Application Example 1

[0137] This application provides an anti-dandruff shampoo formula with triple antibacterial, antioxidant, and antipruritic effects. The antibacterial (anti-dandruff and antipruritic) ingredient is composition 3, which is named Baicui Sujing.

[0138] (1) Formula of Baicui Pure Shampoo

[0139] The weight parts of the components of the shampoo are shown in Table 9, and the preparation method thereof comprises the following steps:

[0140] S1. Add the water of phase A1 to the main pot, add the remaining ingredients of phase A1 while homogenizing, disperse thoroughly, add the ingredients of phase A2, stir thoroughly, heat to 80-85°C, add the ingredients of phase A3, and stir to dissolve completely;

[0141] S2. Add phase B1, stir thoroughly until completely dissolved, then add phase B2 and stir to dissolve completely;

[0142] S3 cool to 45`50 ℃, sequentially add phase C raw materials, stir until transparent;

[0143] S4. Sequentially add the D phase raw materials, stir and cool to room temperature;

[0144] S5. Take samples for testing, and discharge the materials after they pass the test.

[0145] Table 9: Formula of Baicui Clean Shampoo

[0146]

[0147] (2) Volunteer test of Biorefreshing Shampoo

[0148] Thirty male and female volunteers aged 18 to 50 were recruited (eligible volunteers were healthy individuals with oily hair, dandruff, or itchy scalp). They used the shampoo every other day (every other day) for four weeks and were evaluated on three aspects: hair volume, dandruff removal, and itching relief. Each aspect was scored on a scale of 0 to 7 (7 = extremely satisfied, 6 = very satisfied, 5 = somewhat satisfied, 4 = average, 3 = somewhat satisfied, 2 = very dissatisfied, and 1 = extremely dissatisfied). Higher levels of approval were associated with higher scores. Overall satisfaction included those who scored 5 to 7. Approval = overall satisfaction divided by total number of volunteers. The volunteer test results are shown in Table 10.

[0149] Table 10 Test results of Baicuisu pure shampoo

[0150]

[0151] As shown in Table 10, Baicui Sujing Shampoo has good hair volume, dandruff removal and itching relief effects.

[0152] Application Example 2

[0153] This application provides an antipruritic solution with triple effects of antibacterial, antioxidant, and antipruritic. Composition 3 (Baicuisujing) was selected for formulation proofing.

[0154] (1) Formula of Baicuisujing Antipruritic Liquid

[0155] The weight parts of the components of the antipruritic solution are shown in Table 11, and the preparation method thereof comprises the following steps:

[0156] S1. Mix and dissolve phase A, stir evenly and set aside;

[0157] S2. Mix and dissolve phase B, stir evenly, add phase B to phase A, and stir evenly;

[0158] S3. Take samples to test pH, and discharge only those that pass the test.

[0159] Table 11: Formula of Baicuisujing Antipruritic Liquid

[0160]

[0161] (2) Volunteer test of Baicuisujing anti-itching liquid

[0162] Fifteen male and female volunteers aged 18 to 50 were recruited. 0.1% histamine was applied to the arms of the volunteers using a cotton swab. After 15 minutes, symptoms such as wheals, itching, and papules appeared on the volunteers' arms. The effects of the solution were then applied to the volunteers' arms, and the results were recorded over a 20-minute period. The volunteers were scored based on both itching relief and redness (wheal) reduction. Each aspect was scored on a scale of 0 to 7 (7 for extremely satisfied, 6 for very satisfied, 5 for somewhat satisfied, 4 for average, 3 for somewhat dissatisfied, 2 for very dissatisfied, and 1 for extremely dissatisfied). The higher the level of approval, the higher the score. Overall satisfaction includes those who scored 5 to 7, and approval = overall satisfaction divided by total number of participants. The volunteer test results are shown in Table 12.

[0163] Table 12 Test results of Baicuisujing antipruritic liquid

[0164]

[0165] As shown in Table 12, Baicuisujing Antipruritic Liquid has good recognition in relieving itching and removing redness (wheals).

[0166] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant extract having the triple effects of antibacterial, antioxidant and antipruritic, characterized in that: The plant extract consists of a and b; a is Terminalia chebula fruit extract; b is plum fruit extract, flower extract and tribulus terrestris fruit extract; The weight ratio of the plum fruit extract, flower extract and tribulus terrestris fruit extract is 10:7-9:1-3; the flower extract is rose flower extract or rose flower extract; The weight ratio of a and b is 1:

1.

2. The method for preparing the plant extract according to claim 1, wherein The method includes mixing and soaking the plant raw materials of each component and the extractant in a volume ratio of 1:8-12, ultrasonicating, concentrating the filtrate, centrifuging and taking the supernatant to obtain a single plant extract, and mixing according to a formula to obtain the plant extract.

3. A composition having the triple effects of antibacterial, antioxidant and antipruritic, characterized in that: Composed of the plant extract according to claim 1 or the plant extract prepared by the preparation method according to claim 2, and hinokitiol; The weight ratio of the plant extract to hinokitiol is 1-30:

1.

4. The composition according to claim 3, characterized in that The weight ratio of the plant extract to hinokitiol is 1-3:

1.

5. The method for preparing the composition according to claim 3 or 4, characterized in that: The method comprises mixing a hinokitiol solution with a plant extract according to a recipe.

6. The preparation method according to claim 5, characterized in that The concentration of hinokitiol in the hinokitiol alcohol solution is 1-200 mg / mL.

7. Use of the plant extract according to claim 1, the plant extract obtained by the preparation method according to claim 2, the composition according to claim 3 or 4, or the composition obtained by the preparation method according to claim 5 or 6 in preparing a scalp care product or a hair care product.

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