Bacterial enzyme decomposition method of bamboo crude fibers
Cultivating bamboo lysinase groups through synthetic biological methods has solved the problem of indigestion of bamboo crude fibers, achieved efficient conversion of bamboo powder into easy absorption of nutrients, reduced the cost of livestock and poultry aquaculture and the demand for arable land, and improved the health level of animals.
Patent Information
- Application Number
- CN202510593703.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-09
- Publication Date
- 2025-08-08
AI Technical Summary
The feed in traditional livestock, poultry and aquaculture relies on imported high-quality forage and traditional grain, resulting in high breeding costs and increased demand for arable land. At the same time, the crude bamboo fiber is difficult to digest by animals, and effective mycozyme decomposition methods are needed.
The bamboo lysinase population is cultivated through synthetic biological methods, and the proportion of specific Chinese herbal powder and herbal medicines is used, and anaerobic fermentation is carried out in multiple stages. The crude bamboo fiber is converted into easy-to-absorb nutrients and made into bamboo powder for feed.
It improves the nutritional value and digestibility of feed, reduces the use of antibiotics, reduces production costs, regulates the intestinal microecology of animals, and improves the anti-disease immunity and growth performance of animals.
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Figure CN120436207A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of bamboo processing, in particular to a method for decomposing bamboo crude fiber using bacterial enzymes. Background Art
[0002] Bamboo is rich in bamboo flavonoids as well as crude protein, multiple amino acids, total cellulose and other nutrients. The bamboo flavonoids content is 1.39%, the crude protein content is 8.59% on average, it contains 17 kinds of amino acids (8 essential amino acids), total cellulose is 58%, and it does not contain aflatoxin or vomitoxin. Bamboo is the staple food of giant pandas, bamboo lemurs, bamboo worms and bamboo rats. In ancient times, some species became extinct due to food shortages, natural disasters and other reasons. Giant pandas and bamboo lemurs have survived to this day by switching to eating bamboo. Through testing, it was found that the bamboo worms inside the bamboo also have high levels of protein, amino acids, crude fat and unsaturated fatty acids.
[0003] Currently, the large-scale use of antibiotics in traditional livestock, poultry and aquaculture feed has seriously affected the natural environment and human health. In addition, a large amount of high-quality forage (such as alfalfa and oat grass) needs to be imported, which increases breeding costs. At the same time, it also relies on traditional grains (such as corn and soybeans) for production, which in turn increases the demand for arable land. In the process of processing bamboo into feed, the crude fiber of bamboo needs to be converted, otherwise it will be difficult for the animals to digest. Therefore, a method for decomposing the crude fiber of bamboo with bacterial enzymes is needed. Summary of the Invention
[0004] (1) Technical problems solved
[0005] In response to the shortcomings of the existing technology, the present invention provides a method for decomposing bamboo crude fiber with bacterial enzymes. It has the advantages of cultivating a bamboo-decomposing bacterial enzyme group through synthetic biology, converting indigestible components such as bamboo crude fiber for animals into easily absorbable nutrients, thereby improving the nutritional value and digestibility of feed using bamboo ingredients. It solves the current problem of traditional livestock, poultry and aquaculture feed relying on imports and traditional grains, increasing breeding costs and demand for arable land.
[0006] (2) Technical solution
[0007] To achieve the above-mentioned purpose of cultivating bamboo-degrading enzyme groups through synthetic biology, converting indigestible components such as bamboo crude fiber for animals into easily absorbable nutrients, and improving the nutritional value and digestibility of feed using bamboo ingredients, the present invention provides the following technical solution: a method for decomposing bamboo crude fiber with bacterial enzymes, the method comprising:
[0008] Step S1, raw material preparation: rice, wheat, rice husks, and whole bamboo scraps are ground into a 180-200 mesh fine powder, and then 2-2.5 parts of Chinese medicinal powder are added to each 10 parts by weight, mixed together, and 30% of boiled water is added. The mixture is thoroughly stirred and spread evenly on a rack and sieve indoors for 3 days;
[0009] Step S2, cultivating a synthetic bacterial enzyme: drying, crushing, and evenly stirring 100 herbs, then decocting with water for 24 hours, filtering, and adding the filtrate to a culture medium. The filtrate and culture medium are mixed in a weight ratio of 1:2, stirred evenly, and patted by hand into a round cake with a diameter of 6 to 8 cm. The cake is placed on a rack indoors on a mesh screen lined with rice husks or straw for fermentation, and then dried in the sun or oven to synthesize the biocombination bacterial enzyme. The cake is stored in a dry and cool place for future use.
[0010] Step S3, fermentation stage 1: adding 1% synthetic biological combination enzyme, 0.1% each of rhizopus, yeast, molasses, saccharifying enzyme, amylase, protease, esterifying enzyme, and cellulase to 180-200 mesh bamboo powder raw material, and placing in a fermentation tank or fermentation tank for anaerobic fermentation for 7 days;
[0011] Step S4, second fermentation stage: on the 8th day, adding 0.5% of the synthetic biological combination bacterial enzyme, placing the fermentation tank or fermentation tank in a fermentation tank for 7 days of anaerobic fermentation;
[0012] Step S5, the third fermentation stage: On the 15th day, 0.5% of the synthetic biological combination bacterial enzyme is added, and the mixture is placed in a fermentation tank or fermentation tank to continue anaerobic fermentation for 7 days. The bacterial enzyme decomposes the bamboo crude fiber to produce bamboo powder.
[0013] Preferably, the Chinese medicine powder in step S1 comprises the following components, calculated by weight: 6-8 parts of Polygonum hydropiper, 3-6 parts of Lophatherum gracile, 3-6 parts of Licorice root, 3-6 parts of Plantain, 3-5 parts of Morus alba, 2-4 parts of Magnolia officinalis, 2-4 parts of Uncaria rhynchophylla, 1-3 parts of Nanteng, 1-3 parts of Chuanwu, 3-5 parts of Epimedium, 5-7 parts of Pogostemon cablin, 3-5 parts of malt, 0.5-2 parts of Chrysanthemum morifolium, 3-5 parts of Hawthorn, and 3-5 parts of Euryale ferox, which are then mixed together and ground into 80-120 mesh Chinese medicine powder.
[0014] Preferably, the Chinese medicine powder further comprises 0.1% each of rhizopus, yeast, molasses, saccharifying enzyme, amylase, protease, esterifying enzyme and cellulase.
[0015] Preferably, the 100 herbs in step S2 are calculated by weight and include the following components: 1 to 2 parts each of seven-leaf atractylodes, three-leaf acanthopanax, millennium jian, small red garlic, large folic acid vine, native beehive, purslane, kapok, small red ginseng, nightshade, dragon blood tree, whip ginger, safflower, mouse cucumber, bamboo leaf pepper, bamboo yellow, aloe, houttuynia cordata, dog spine, cyperus, camphor leaf, pearl grass, fennel, amomum villosum, wide tendon vine, wild ginger, wild eggplant, wild licorice, wild pepper, perilla, kabu Luoxiang, sandalwood, and snake intestine; 1 to 2 parts each of crown fern, Yunnan schisandra, calamus, cypress, rootless vine, white flower dandelion, head flower curculigo, bamboo leaf orchid, two-sided needle, cockscomb, chicken arrow vine, primrose, sappan wood, green 2-4 parts each of box, golden flower fruit, bitter vine, buckwheat, caltrop, midden wood, blood-tonifying fragrance, secret vine, yellow Myanmar osmanthus, Wang Jiangnan, basella, sausage tree, cricket grass, qilin leaf, secret vine, mountain jute, large stemona, large plantain, large comfrey, large-leaf vernose, verbena; 4-8 parts each of thousand-year-old paper, mountain sesame, fennel, large bone dan, red deer grass, fruit vine, large blood vine, water willow, pigeon pea, diarrhea wood, raw vine, tiger taro, green ox gall, dog tooth flower, heart-setting vine, southern thousand-jin vine, yellow camphor, black skin trauma, nine-winged cardamom; 6-12 parts each of wood milk fruit, stone mandarin orange, white powder vine, white crane ganoderma, double-leaf fig, corn cob, sugarcane, green vine, caviar, kidney tea, wind-removing grass, coix.
[0016] Preferably, the indoor temperature in step S2 is required to be 25-28° C., and the fermentation time is 20-22 days.
[0017] Preferably, the temperature of the fermentation tank or fermentation vessel in step S3 is required to be 25-28°C.
[0018] Preferably, the temperature of the fermentation tank or fermentation vessel in step S4 is required to be 28-35°C.
[0019] Preferably, the temperature of the fermentation tank or fermentation vessel in step S5 is required to be 45-55°C.
[0020] (3) Beneficial effects
[0021] Compared with the prior art, the present invention provides a method for decomposing bamboo crude fiber by bacterial enzymes, which has the following beneficial effects:
[0022] The method for decomposing bamboo crude fiber with bacterial enzymes cultivates bamboo-decomposing bacterial enzyme groups through synthetic biology, converts indigestible components such as bamboo crude fiber for animals into easily absorbable nutrients, improves the nutritional value and digestibility of feed using bamboo ingredients, significantly reduces the use of antibiotics in feed, reduces the production cost of finished feed, reduces the demand for traditional feed raw materials and staple foods, thereby reducing imported forage and breeding costs, and at the same time saccharifies it to increase protein and fat. In addition, through synthetic biological bacterial enzyme technology, the effective ingredients and live probiotics in bamboo can be utilized. By using bamboo instead of grain to develop anti-resistant feed, the intestinal microecological balance of livestock, poultry, fish, shrimp, crab, ginseng and shellfish can be effectively regulated, and the disease resistance, immunity and growth performance of livestock, poultry, fish, shrimp, crab, ginseng and shellfish can be effectively improved. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 This is a schematic flow chart of a method for decomposing bamboo crude fiber using bacterial enzymes proposed in the present invention;
[0024] Figure 2 This is the content table of the original unfermented common bamboo proposed in the present invention;
[0025] Figure 3 This is a nutritional test table for the finished product of the bacterial enzyme decomposition method of bamboo crude fiber proposed in the present invention;
[0026] Figure 4 This is a metabolic digestibility test table for the finished product of the bacterial enzyme decomposition method of bamboo crude fiber proposed in the present invention. DETAILED DESCRIPTION
[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0028] See also Figure 1 A method for decomposing bamboo crude fiber by bacterial enzymes, the method comprising:
[0029] Step S1, raw material preparation: Grind rice, wheat, rice husks, and whole bamboo chips together into 180-200 mesh fine powder, and then add 2-2.5 parts of Chinese medicinal powder by weight for every 10 parts and mix them together, wherein the Chinese medicinal powder is calculated by weight and includes the following components: 6-8 parts of Polygonum hydropiper, 3-6 parts of Bambusa chinensis, 3-6 parts of Licorice root, 3-6 parts of Plantain, 3-5 parts of Morus alba, 2-4 parts of Magnolia officinalis, 2-4 parts of Uncaria rhynchophylla, and 1-3 parts of Nanteng. , 1-3 parts of Chuanwu, 3-5 parts of Epimedium, 5-7 parts of Atractylodes lancea, 3-5 parts of malt, 0.5-2 parts of chrysanthemum, 3-5 parts of hawthorn, 3-5 parts of Euryale ferox, and then grind them together into 80-120 mesh Chinese medicine powder, the Chinese medicine powder also includes 0.1% of rhizopus, yeast, molasses, saccharifying enzyme, amylase, protease, esterifying enzyme and cellulase, and then add 30% of boiled water, stir well, and place on a rack and sieve indoors for 3 days;
[0030] Step S2, cultivating synthetic bacterial enzyme: 100 herbs are calculated by weight, including the following components: seven-leaf stalk flower, three-leaf aralia, thousand-year-old garlic, large folic acid vine, native beehive, purslane, kapok, small red ginseng, nightshade, dragon blood tree, whip-shaped ginger, safflower, mouse cucumber, bamboo leaf pepper, bamboo yellow, aloe, houttuynia cordata, dog spine, cyperus, camphor leaf, pearl grass, fennel, wide tendon vine, wild ginger, wild eggplant, wild licorice, wild pepper, perilla, kabuki, sandalwood, snake intestine 1-2 parts each; 2-4 parts each of crown fern, schisandra yunnanensis, calamus, ox-bone root, rootless vine, plum blossom, curculigo orchioides, bamboo orchid, two-sided needle, cockscomb, cockscomb, primrose, sappan wood, green box, golden flower fruit, bitter vine, buckwheat, calamus, medlar, tongxiexiang, secret fat vine, yellow Myanmar cinnamon, wangjiangnan, basella, sausage tree, cricket grass, unicorn leaf, secret fat vine, mountain jute, large stemona, large plantain, large comfrey, large leaf vernonia, verbena; thousand-year paper, mountain sesame, fennel 4-8 parts each of da bogudan, red deer grass, lower fruit vine, large blood vine, water willow, pigeon bean, diarrhea wood, raw vine, tiger taro, green ox gall, dog tooth flower, heart vine, southern thousand jin vine, yellow camphor, black skin trauma, nine-winged cardamom; 6-12 parts each of wood milk fruit, tangerine, white powder vine, white crane ganoderma, opposite leaf fig, corn cob, sugarcane, green vine, caviar orchid, kidney tea, wind-clearing grass, coix. Dry the above 100 herbs, crush them, stir them evenly, boil them with water for 24 hours, filter them, and take the filtrate. Add the culture medium and mix the filtrate with the culture medium in a weight ratio of 1:2, stir evenly, pat it into a round cake with a diameter of 6 to 8 cm by hand, and spread it on a mesh screen with rice husks or straw as the base for fermentation indoors. The indoor temperature is required to be 25 to 28°C, and the fermentation time is 20 to 22 days. Then, dry it in the sun or bake it to synthesize the bio-combination enzyme, store it in a dry and cool place for future use, and effectively solve the carbon source and nitrogen source necessary for biological fermentation through the addition of Chinese medicinal materials, so as to play a saccharification role.
[0031] Step S3, fermentation stage 1: adding 1% synthetic biological combination enzyme, 0.1% rhizopus, yeast, molasses, saccharifying enzyme, amylase, protease, esterase, and cellulase to the 180-200 mesh bamboo powder raw material in step 1, and placing the raw material in a fermentation tank or fermentation tank for anaerobic fermentation for 7 days at a temperature of 25-28° C.;
[0032] Step S4, fermentation second stage: on the 8th day, add 0.5% of the synthetic biological combination bacterial enzyme, place in the fermentation tank or fermentation tank for 7 days of anaerobic fermentation, the temperature of the fermentation tank or fermentation tank is required to be 28-35°C;
[0033] Step S5, third fermentation stage: On the 15th day, 0.5% of the synthetic biological combination bacterial enzyme is added, and the mixture is placed in a fermentation tank or fermentation vessel and anaerobic fermentation is continued for 7 days. The temperature of the fermentation tank or fermentation vessel is required to be 45-55° C. The bacterial enzyme decomposes the bamboo crude fiber to produce bamboo powder. This bamboo powder can be used as a bamboo feed substitute to replace 20-80% of the main feed corn. It can also be added to low-carbohydrate, low-fat, and low-sugar foods at 10-30%.
[0034] In summary, with reference Figures 2 to 4 The method for decomposing bamboo crude fiber with bacterial enzymes cultivates bamboo-dissolving bacterial enzyme groups through synthetic biological methods, converts the indigestible components of bamboo crude fiber and other components for animals into easily absorbable nutrients, and at the same time saccharifies them, increases protein and fat, and improves the nutritional value and digestibility of feed using bamboo ingredients, greatly reducing the use of antibiotics in feed, reducing the production cost of finished feed, and reducing the demand for traditional feed raw materials and staple foods. By taking advantage of the low land requirements of bamboo itself, the demand for arable land can be reduced, and imported forage can be reduced, reducing breeding costs. In addition, through synthetic biological bacterial enzyme technology, the effective ingredients and live probiotics in bamboo can be utilized, and by developing anti-resistant feed with bamboo instead of grain, the intestinal microecological balance of livestock, poultry, fish, shrimp, crab, ginseng and shellfish can be effectively regulated, and the disease resistance, immunity and growth performance of livestock, poultry, fish, shrimp, crab, ginseng and shellfish can be effectively improved.
[0035] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a" does not exclude the presence of additional identical elements in the process, method, article, or device comprising the element.
[0036] In this application, terms such as "upper," "lower," "inner," "middle," "outer," "front," and "back" indicate positions or locations based on the positions or locations shown in the accompanying drawings. These terms are primarily intended to better describe this application and its embodiments and are not intended to limit the devices, elements, or components indicated to having a specific orientation, or to being constructed or operated in a specific orientation.
[0037] Furthermore, some of the above terms may be used to express other meanings besides indicating a position or location. For example, the term "on" may also be used to indicate a dependency or connection in certain circumstances. Those skilled in the art will understand the specific meanings of these terms in this application based on the specific circumstances.
[0038] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.
Claims
1. A method for decomposing bamboo crude fiber by bacterial enzyme, characterized in that: The method includes: Step S1, raw material preparation: rice, wheat, rice husks, and whole bamboo scraps are ground into a 180-200 mesh fine powder, and then 2-2.5 parts of Chinese medicinal powder are added to each 10 parts by weight, mixed together, and 30% of boiled water is added. The mixture is thoroughly stirred and spread evenly on a rack and sieve indoors for 3 days; Step S2, cultivating a synthetic bacterial enzyme: drying, crushing, and evenly stirring 100 herbs, then decocting with water for 24 hours, filtering, and adding the filtrate to a culture medium. The filtrate and culture medium are mixed in a weight ratio of 1:2, stirred evenly, and patted by hand into a round cake with a diameter of 6 to 8 cm. The cake is placed on a rack indoors on a mesh screen lined with rice husks or straw for fermentation, and then dried in the sun or oven to synthesize the biocombination bacterial enzyme. The cake is stored in a dry and cool place for future use. Step S3, fermentation stage 1: adding 1% synthetic biological combination enzyme, 0.1% each of rhizopus, yeast, molasses, saccharifying enzyme, amylase, protease, esterifying enzyme, and cellulase to 180-200 mesh bamboo powder raw material, and placing in a fermentation tank or fermentation tank for anaerobic fermentation for 7 days; Step S4, second fermentation stage: on the 8th day, adding 0.5% of the synthetic biological combination bacterial enzyme, placing the fermentation tank or fermentation tank in a fermentation tank for 7 days of anaerobic fermentation; Step S5, the third fermentation stage: On the 15th day, 0.5% of the synthetic biological combination bacterial enzyme is added, and the mixture is placed in a fermentation tank or fermentation tank to continue anaerobic fermentation for 7 days. The bacterial enzyme decomposes the bamboo crude fiber to produce bamboo powder.
2. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 1, characterized in that: The Chinese medicinal powder in step S1 is calculated by weight and includes the following components: 6-8 parts of Polygonum hydropiper, 3-6 parts of Lophatherum gracile, 3-6 parts of Licorice root, 3-6 parts of Plantain, 3-5 parts of Morus alba, 2-4 parts of Magnolia officinalis, 2-4 parts of Uncaria rhynchophylla, 1-3 parts of Rhizoma Aconiti Lateralis Preparata, 1-3 parts of Radix Aconiti Lateralis Preparata, 3-5 parts of Epimedium, 5-7 parts of Pogostemon cablin, 3-5 parts of Malt, 0.5-2 parts of Chrysanthemum morifolium, 3-5 parts of Crataegus pinnatifida, and 3-5 parts of Euryale ferox. The components are then mixed together and ground into Chinese medicinal powder of 80-120 mesh.
3. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 2, characterized in that: The traditional Chinese medicine powder further comprises 0.1% of rhizopus, yeast, molasses, saccharifying enzyme, amylase, protease, esterifying enzyme and cellulase.
4. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 1, characterized in that: The 100 herbs in step S2 are calculated by weight and include the following components: 1 to 2 parts each of seven-leaf hibiscus flower, three-leaf euphorbia, millennium jian, small red garlic, large folic acid vine, native beehive, purslane, kapok, small red ginseng, nightshade, dragon blood tree, whip-shaped ginger, safflower, mouse cucumber, bamboo leaf pepper, bamboo yellow, aloe, houttuynia cordata, dog spine, cyperus, camphor leaf, pearl grass, fennel, amomum villosum, wide tendon vine, wild ginger, wild eggplant, wild licorice, wild pepper, perilla, kabu Luoxiang, sandalwood, and snake intestine; 1 to 2 parts each of crown fern, Yunnan schisandra, calamus, cypress, rootless vine, white flower dandelion, head flower curculigo, bamboo leaf orchid, two-sided needle, cockscomb, chicken arrow vine, primrose, sappan wood, green box , golden flower fruit, bitter vine, buckwheat, caltrop, midden wood, blood-tonifying fragrance, secret vine, yellow Myanmar osmanthus, Wang Jiangnan, basella, sausage tree, cricket grass, qilin leaf, secret vine, mountain jute, large stemona, large plantain, large comfrey, large-leafed vernose, verbena, 2-4 parts each; thousand-year paper, mountain sesame, fennel, large bone Dan, red deer grass, fruit vine, large blood vine, water willow, pigeon pea, diarrhea wood, raw vine, tiger taro, green ox gall, dog tooth flower, heart-setting vine, southern thousand-jin vine, yellow camphor, black skin trauma, nine-winged cardamom, wood milk fruit, stone orange, white powder vine, white crane Ganoderma lucidum, double-leaf fig, corn cob, sugarcane, green vine, caviar, kidney tea, wind-removing grass, coix seed, 6-12 parts each.
5. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 1, characterized in that: The indoor temperature in step S2 is required to be 25-28° C., and the fermentation time is 20-22 days.
6. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 1, characterized in that: The temperature of the fermentation tank or fermentation vessel in step S3 is required to be 25-28°C.
7. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 1, characterized in that: The temperature of the fermentation tank or fermentation vessel in step S4 is required to be between 28°C and 35°C.
8. The method for decomposing bamboo crude fiber by bacterial enzyme according to claim 1, characterized in that: The temperature of the fermentation tank or fermentation vessel in step S5 is required to be 45-55°C.