Cell bioreactor and use method thereof

Abstract

The invention discloses a cell bioreactor and a use method thereof, and relates to the technical field of cell biologication.The cell bioreactor comprises a workbench, a box is fixedly connected to the bottom face of the workbench, a through groove is formed in the middle of the top face of the workbench, and a transverse plate is fixedly connected to the middle of the through groove; a liquid mixing box is arranged on the top face of the workbench, and a box door is arranged on the top of the liquid mixing box. The device further comprises a sliding assembly, limiting assemblies and a driving assembly, the sliding assembly is arranged on the workbench and used in cooperation with the liquid mixing box, the limiting assemblies are arranged on the two sides of the liquid mixing box and used in cooperation with the liquid mixing box, and the driving assembly is arranged on the transverse plate and used for moving the liquid mixing box back and forth. The device is reasonable in structure, the bracket moves back and forth through the driving assembly, and then cells in the liquid mixing box and a culture solution are mixed more uniformly through cooperative use of the sliding assembly and the limiting assembly.

Description

Technical Field

[0001] The present invention relates to the field of cell biotechnology, and in particular to a cell bioreactor and a method for using the same. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH, and nutritional conditions) in vitro to enable cells to survive, grow, reproduce, and maintain their primary structures and functions. Cell culture, also known as cell cloning technology, is formally referred to in biology as cell culture technology. Cell culture technology is an important and commonly used technique in cell biology research. It allows for the production of large numbers of cells and allows for the study of cell signal transduction, cell metabolism, and cell growth and proliferation.

[0003] The announcement number CN222294094U is a cell bioreactor, including a bottom plate, side frames are fixed on both sides of the top surface of the bottom plate, a limiting groove is provided on the top of the inner wall of the side frame, a toothed ring is rotatably connected in the limiting groove on one side, and a limiting ring is rotatably connected in the limiting groove on the other side; the first stirring rod provided in the utility model enables the culture fluid and cells to be further mixed, and the second stirring rod provided enables the culture fluid and cells entering the mixing tube on both sides to be mixed, so that the cells and the culture fluid are fully contacted and mixed for reaction, thereby improving the density of the added cells and avoiding the situation where the operator's arm pain is easily caused by manually shaking the reaction tube for a long time. The above patent mixes the cells and the culture fluid by rotation, and this mixing method has a poor mixing effect. Summary of the Invention

[0004] The purpose of this application is to provide a cell bioreactor and a method for using the same, wherein a driving assembly is used to move the bracket back and forth, and then a sliding assembly and a limiting assembly are used in conjunction to facilitate more uniform mixing of cells and culture fluid inside a mixing tank.

[0005] To achieve the above-mentioned objectives, the present application provides the following technical solutions: a cell bioreactor and a method for using the same, comprising a workbench, wherein the bottom surface of the workbench is fixedly connected to a box body, a through groove is provided in the middle position of the top surface of the workbench, the middle position of the through groove is fixedly connected to a horizontal plate, a mixing box is provided at the top surface of the workbench, and a box door is installed at the top position of the mixing box; further comprising a sliding assembly, a limiting assembly and a driving assembly, wherein the sliding assembly is arranged on the workbench for use in conjunction with the mixing box, the limiting assembly is arranged at both sides of the mixing box for use in conjunction, and the driving assembly is arranged on the horizontal plate for moving the mixing box back and forth.

[0006] Preferably, the sliding assembly includes sliding blocks fixedly mounted on both sides of the liquid mixing box, the sliding blocks are slidably connected to slide rails, and the slide rails are fixedly mounted on the inner wall of the bracket.

[0007] Preferably, side panels are fixedly connected to both sides of the bracket, pulleys are rotatably connected to the side panels, the pulleys are slidably connected to the slide rod, both ends of the slide rod are fixedly connected to the support seat, the bottom surface of the support seat is fixedly connected to the workbench, the front and rear sides of the workbench are fixedly connected to the support plates, and the top of the support plate is fixedly connected to the positioning plate.

[0008] Preferably, the limiting assembly includes a top plate fixedly installed on both sides of the mixing box, the bottom position of the top plate is fixedly connected to a driven plate, the middle position of the outer surface of the driven plate is rotatably connected to a first roller, and the first roller is attached to the top position of the positioning plate.

[0009] Preferably, an axle seat is fixedly connected to the inner wall of the driven plate, a limiting rod is fixedly connected to the axle seat, a sleeve plate is slidably connected to the limiting rod, a sleeve ring is fixedly connected to the bottom end of the limiting rod, a first spring is sleeved on the limiting rod, and both ends of the first spring are fixedly connected to the top surface of the sleeve ring and the bottom surface of the sleeve plate respectively.

[0010] Preferably, a docking plate is fixedly connected to the sleeve plate, a second roller is rotatably connected to the docking plate, and the second roller is slidably connected to the bottom surface of the positioning plate.

[0011] Preferably, the driving assembly includes a base plate fixedly mounted on the bottom surface of the horizontal plate, a pair of limit plates fixedly connected to the base plate, a pair of limit plates are provided with guide grooves, a cross bar is slidably connected inside the guide groove, and a gear is fixedly connected to the middle position of the cross bar.

[0012] Preferably, the bottom surface of the gear is meshed with the first tooth plate, the first tooth plate is fixedly mounted on the transverse plate, the top surface of the gear is meshed with the second tooth plate, and the second tooth plate is fixedly connected to the bottom surface of the bracket.

[0013] Preferably, both ends of the cross bar are rotatably connected to a linkage plate, the other end of the linkage plate is rotatably connected to the arm plate, the top of the arm plate is fixedly connected to the main shaft, one end of the main shaft is fixedly connected to the output end of the drive motor, the drive motor is fixedly mounted on the frame, and the bottom end of the frame is fixedly connected to the cross plate.

[0014] The present invention also provides a method for using a cell bioreactor, comprising: Cells and culture medium are added to the interior of the mixing box, and then the bracket is moved back and forth through the operation of the drive component. The movement of the bracket drives the mixing box to move up and down, so that the cells and culture medium inside the mixing box are mixed more evenly.

[0015] In summary, the present invention has the following beneficial effects: 1. The present invention has a reasonable structure. When the mixing tank needs to be moved, the bracket is moved by the driving assembly. Side plates are fixedly connected to both sides of the bracket, and the side plates are rotatably connected to the pulleys. The movement of the bracket causes the pulleys to slide on the slide rods, thereby increasing the stability of the bracket movement. 2. In the present invention, when the bracket moves, top plates are fixedly connected to both sides of the mixing box, a driven plate is fixedly connected to the bottom surface of the top plate, an axle seat is fixedly connected to the driven plate, and a limit rod is fixedly connected to the axle seat. The elastic force of the first spring causes the second roller on the docking plate to fit the bottom surface of the positioning plate. The top surface of the positioning plate is wavy. The movement of the mixing box causes the first roller to slide up and down along the edge of the top surface of the positioning plate, thereby facilitating the mixing box to move up and down when moving left and right, so that the cells and culture medium inside the mixing box are mixed more evenly. 3. In the present invention, when the bracket moves back and forth, the driving motor operates to rotate the main shaft, and the rotation of the main shaft drives the linkage plate to move through the arm plate. The other end of the linkage plate is rotatably connected to the cross bar, and the movement of the linkage plate pushes the cross bar to slide inside the guide groove. The back and forth movement of the cross bar causes the gear to drive the second gear plate to move, thereby facilitating the back and forth movement of the bracket as a whole. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 Schematic diagram of the three-dimensional structure of the workbench; Figure 2 It is a schematic diagram of the three-dimensional structure of a partial section of the box; Figure 3 Schematic diagram of the three-dimensional structure of the liquid mixing box; Figure 4 This is a schematic diagram of the three-dimensional structure of the liquid mixing box when viewed from above; Figure 5 Schematic diagram of the three-dimensional structure of the bracket; Figure 6 This is a schematic diagram of the three-dimensional structure of the bracket when viewed from above; Figure 7 Schematic diagram of the three-dimensional structure of the horizontal plate; Figure 8 for Figure 4 Enlarged structural diagram at point A in the middle.

[0018] In the figure: 1. Workbench; 101. Box body; 102. Through slot; 103. Horizontal plate; 104. Mixing box; 105. Box door; 2. Slider; 201. Slide rail; 202. Bracket; 203. Side plate; 204. Pulley; 205. Sliding rod; 206. Support seat; 207. Support plate; 208. Positioning plate; 3. Top plate; 301. Driven plate; 302. First roller; 303. Axle seat; 304, limit rod; 305, sleeve plate; 306, sleeve ring; 307, first spring; 308, docking plate; 309, second roller; 4, bottom plate; 401, limit plate; 402, guide groove; 403, cross bar; 404, gear; 405, first tooth plate; 406, second tooth plate; 407, linkage plate; 408, arm plate; 409, main shaft; 410, drive motor; 411, frame. DETAILED DESCRIPTION

[0019] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] Example: Reference Figure 1 - Figure 8 A cell bioreactor and a method of using the same are shown, comprising a workbench 1, the bottom of which is fixedly connected to a box body 101, a through slot 102 being provided in the middle of the top of the workbench 1, a cross plate 103 being fixedly connected in the middle of the through slot 102, a mixing liquid box 104 being provided on the top of the workbench 1, and a box door 105 being installed on the top of the mixing liquid box 104; further comprising a sliding assembly, a limiting assembly and a driving assembly, the sliding assembly being provided on the workbench 1 for use in conjunction with the mixing liquid box 104, the limiting assemblies being provided on both sides of the mixing liquid box 104 for use in conjunction, and the driving assembly being provided on the cross plate 103 for moving the mixing liquid box 104 back and forth.

[0021] Specifically, it should be noted that the driving motor 410 is electrically connected to the control panel via a wire, and the specific working principles thereof are referenced from the existing technology and will not be elaborated on herein.

[0022] As an implementation method in this embodiment, the sliding assembly includes sliders 2 fixedly installed on both sides of the mixing box 104, the sliders 2 are slidably connected to the slide rails 201, the slide rails 201 are fixedly installed on the inner wall of the bracket 202, and the side plates 203 are fixedly connected on both sides of the bracket 202. The pulleys 204 are rotatably connected to the side plates 203, and the pulleys 204 are slidably connected to the slide rod 205. The two ends of the slide rod 205 are fixedly connected to the support seat 206, and the bottom surface of the support seat 206 is fixedly connected to the workbench 1. The front and rear sides of the workbench 1 are fixedly connected to the support plates 207, and the top of the support plate 207 is fixedly connected to the positioning plate 208.

[0023] Specifically, when the mixing box 104 needs to be moved, the bracket 202 is moved by the driving assembly. Side plates 203 are fixedly connected to both sides of the bracket 202, and the side plates 203 are rotatably connected to the pulleys 204. The movement of the bracket 202 causes the pulleys 204 to slide on the slide rods 205, thereby increasing the stability of the movement of the bracket 202.

[0024] As an implementation scheme in this embodiment, the limiting assembly includes a top plate 3 fixedly installed on both sides of the mixing liquid box 104, the bottom position of the top plate 3 is fixedly connected to a driven plate 301, the middle position of the outer surface of the driven plate 301 is rotatably connected to the first roller 302, the first roller 302 is affixed to the top position of the positioning plate 208, the inner wall of the driven plate 301 is fixedly connected to an axle seat 303, the axle seat 303 is fixedly connected to a limiting rod 304, the limiting rod 304 is slidably connected to a sleeve plate 305, the bottom end of the limiting rod 304 is fixedly connected to a sleeve ring 306, the limiting rod 304 is sleeved with a first spring 307, the two ends of the first spring 307 are respectively fixedly connected to the top surface of the sleeve ring 306 and the bottom surface of the sleeve plate 305, the sleeve plate 305 is fixedly connected to a docking plate 308, the docking plate 308 is rotatably connected to a second roller 309, and the second roller 309 is slidably connected to the bottom surface of the positioning plate 208.

[0025] Specifically, when the bracket 202 moves, a top plate 3 is fixedly connected to both sides of the mixing box 104, a driven plate 301 is fixedly connected to the bottom surface of the top plate 3, an axle seat 303 is fixedly connected to the driven plate 301, and a limiting rod 304 is fixedly connected to the axle seat 303. The elastic force of the first spring 307 makes the second roller 309 on the docking plate 308 fit into the bottom surface of the positioning plate 208. The top surface of the positioning plate 208 is wavy. The movement of the mixing box 104 makes the first roller 302 slide up and down along the edge of the top surface of the positioning plate 208, so that the mixing box 104 can move up and down when moving left and right, so that the cells and culture medium inside the mixing box 104 are mixed more evenly.

[0026] As an implementation method in this embodiment, the driving assembly includes a bottom plate 4 fixedly mounted on the bottom surface of the horizontal plate 103, a pair of limit plates 401 are fixedly connected to the bottom plate 4, a pair of limit plates 401 are provided with guide grooves 402, the interior of the guide grooves 402 is slidably connected to a cross bar 403, the middle position of the cross bar 403 is fixedly connected to a gear 404, the bottom surface of the gear 404 is meshed with the first tooth plate 405, the first tooth plate 405 is fixedly mounted on the horizontal plate 103, and the gear 404 is engaged with the first tooth plate 405. The top surface position is engaged with the second tooth plate 406, and the second tooth plate 406 is fixedly connected to the bottom surface position of the bracket 202. The two ends of the cross bar 403 are rotatably connected to the linkage plate 407, and the other end of the linkage plate 407 is rotatably connected to the arm plate 408. The top end of the arm plate 408 is fixedly connected to the main shaft 409, and one end of the main shaft 409 is fixedly connected to the output end of the drive motor 410. The drive motor 410 is fixedly installed on the frame 411, and the bottom end of the frame 411 is fixedly connected to the cross plate 103.

[0027] Specifically, when the bracket 202 moves back and forth, the drive motor 410 operates to rotate the main shaft 409. The rotation of the main shaft 409 drives the linkage plate 407 to move through the arm plate 408. The other end of the linkage plate 407 is rotatably connected to the cross bar 403. The movement of the linkage plate 407 pushes the cross bar 403 to slide inside the guide groove 402. The back and forth movement of the cross bar 403 causes the gear 404 to drive the second gear plate 406 to move, thereby facilitating the back and forth movement of the bracket 202 as a whole.

[0028] The working principle of the present invention is as follows: when the mixing box 104 needs to be moved, the bracket 202 is moved by the driving assembly. Side plates 203 are fixedly connected to both sides of the bracket 202, and the side plates 203 are rotatably connected to the pulleys 204. The movement of the bracket 202 causes the pulleys 204 to slide on the slide rods 205, thereby increasing the stability of the movement of the bracket 202. When the bracket 202 moves, the top plates 3 are fixedly connected to both sides of the mixing box 104, the bottom surface of the top plate 3 is fixedly connected to the driven plate 301, the driven plate 301 is fixedly connected to the shaft seat 303, and the shaft seat 303 is fixedly connected to the limiting rod 304, and the elastic force of the first spring 307 makes the second roller 309 on the docking plate 308 fit the bottom surface position of the positioning plate 208. The top surface of the positioning plate 208 is wavy. The movement of the mixing box 104 makes the first roller 302 slide up and down along the edge of the top surface of the positioning plate 208, so that the mixing box 104 can move up and down when it moves left and right, so that the cells and culture medium inside the mixing box 104 are mixed more evenly. When the bracket 202 moves back and forth, the drive motor 410 operates to rotate the main shaft 409. The rotation of the main shaft 409 drives the linkage plate 407 to move through the arm plate 408. The other end of the linkage plate 407 is rotatably connected to the cross bar 403. The movement of the linkage plate 407 pushes the cross bar 403 to slide inside the guide groove 402. The back and forth movement of the cross bar 403 causes the gear 404 to drive the second gear plate 406 to move, thereby facilitating the back and forth movement of the bracket 202 as a whole.

[0029] The present invention also provides a method for using a cell bioreactor, comprising: The two ends of the cross bar 403 are rotatably connected to the linkage plate 407, and the other end of the linkage plate 407 is rotatably connected to the arm plate 408. The top of the arm plate 408 is fixedly connected to the main shaft 409. One end of the main shaft 409 is fixedly connected to the output end of the drive motor 410. The drive motor 410 is fixedly installed on the frame 411, and the bottom end of the frame 411 is fixedly connected to the cross plate 103.

[0030] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cell bioreactor, characterized in that include A workbench (1), wherein a box body (101) is fixedly connected to the bottom surface of the workbench (1), a through slot (102) is provided at the middle position of the top surface of the workbench (1), a horizontal plate (103) is fixedly connected to the middle position of the through slot (102), a mixing liquid box (104) is provided at the top surface of the workbench (1), and a box door (105) is installed at the top position of the mixing liquid box (104); It also includes a sliding component, a limiting component and a driving component, wherein the sliding component is arranged on the workbench (1) for use in conjunction with the liquid mixing box (104), the limiting components are arranged at both sides of the liquid mixing box (104) for use in conjunction, and the driving component is arranged on the transverse plate (103) for moving the liquid mixing box (104) back and forth.

2. A cell bioreactor according to claim 1, characterized in that: The sliding assembly comprises sliding blocks (2) fixedly mounted on both sides of the liquid mixing box (104), the sliding blocks (2) being slidably connected to sliding rails (201), and the sliding rails (201) being fixedly mounted on the inner wall of the bracket (202).

3. A cell bioreactor according to claim 2, characterized in that: Side plates (203) are fixedly connected to both sides of the bracket (202), pulleys (204) are rotatably connected to the side plates (203), the pulleys (204) are slidably connected to the slide rod (205), both ends of the slide rod (205) are fixedly connected to the support seat (206), the bottom surface of the support seat (206) is fixedly connected to the workbench (1), the front and rear sides of the workbench (1) are fixedly connected to support plates (207), and the top of the support plate (207) is fixedly connected to a positioning plate (208).

4. A cell bioreactor according to claim 3, characterized in that: The limiting assembly comprises a top plate (3) fixedly mounted on both sides of the liquid mixing box (104); a driven plate (301) is fixedly connected to the bottom of the top plate (3); a first roller (302) is rotatably connected to the middle of the outer surface of the driven plate (301); and the first roller (302) is attached to the top of the positioning plate (208).

5. A cell bioreactor according to claim 4, characterized in that: The inner wall of the driven plate (301) is fixedly connected to a shaft seat (303), the shaft seat (303) is fixedly connected to a limiting rod (304), a sleeve plate (305) is slidably connected to the limiting rod (304), a sleeve ring (306) is fixedly connected to the bottom end of the limiting rod (304), a first spring (307) is sleeved on the limiting rod (304), and two ends of the first spring (307) are fixedly connected to the top surface of the sleeve ring (306) and the bottom surface of the sleeve plate (305), respectively.

6. A cell bioreactor according to claim 5, characterized in that: A docking plate (308) is fixedly connected to the sleeve plate (305), a second roller (309) is rotatably connected to the docking plate (308), and the second roller (309) is slidably connected to the bottom surface of the positioning plate (208).

7. A cell bioreactor according to claim 2, characterized in that: The driving assembly comprises a bottom plate (4) fixedly mounted on the bottom surface of the transverse plate (103); a pair of limiting plates (401) are fixedly connected to the bottom plate (4); a pair of limiting plates (401) are provided with guide grooves (402); a transverse rod (403) is slidably connected inside the guide groove (402); and a gear (404) is fixedly connected to the middle position of the transverse rod (403).

8. A cell bioreactor according to claim 7, characterized in that: The bottom surface of the gear (404) is meshed with a first tooth plate (405), and the first tooth plate (405) is fixedly mounted on the transverse plate (103). The top surface of the gear (404) is meshed with a second tooth plate (406), and the second tooth plate (406) is fixedly connected to the bottom surface of the bracket (202).

9. A cell bioreactor according to claim 8, characterized in that: Both ends of the crossbar (403) are rotatably connected to a linkage plate (407), the other end of the linkage plate (407) is rotatably connected to an arm plate (408), the top end of the arm plate (408) is fixedly connected to a main shaft (409), one end of the main shaft (409) is fixedly connected to an output end of a drive motor (410), the drive motor (410) is fixedly mounted on a frame (411), and the bottom end of the frame (411) is fixedly connected to the crossbar (103).

10. A method for using a cell bioreactor, based on any one of claims 1 to 9, comprising: Cells and culture fluid are added to the interior of the mixing box (104), and then the bracket (202) is moved back and forth by the operation of the driving component. The movement of the bracket (202) drives the mixing box (104) to move up and down, thereby making the cells and culture fluid inside the mixing box (104) more evenly mixed.

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