Natural inducer for enhancing plant immunity to resist tomato early blight and preparation process thereof

Abstract

The invention discloses a natural inducer for enhancing plant immunity to resist tomato early blight and a preparation process thereof. According to the invention, through scientific proportioning of the potassium glutamate, the S-allyl-L-cysteine, the 2-piperidinecarboxylic acid and other key components, the intrinsic immune mechanism of tomato plants is effectively stimulated. The potassium glutamate serving as amino acid salt not only provides nutrition required by plant growth, but also improves the resistance of plants to pathogenic bacteria by promoting cell division and enhancing cell wall strength. As a bioactive substance, S-allyl-L-cysteine can induce plants to generate disease-resistant related enzymes, such as chitinase and beta-1, 3-glucanase, and the enzymes can decompose cell walls of pathogenic bacteria so as to inhibit infection and diffusion of the pathogenic bacteria. 2-piperidinecarboxylic acid further strengthens the immune response of the plants by regulating the hormone balance in the plants, so that the tomato plants can quickly respond when facing early blight, and the occurrence and spreading of diseases are reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of tomato early blight prevention and treatment, and specifically relates to a natural inducer for enhancing plant immunity to resist tomato early blight and a preparation process thereof. Background Art

[0002] Tomato early blight, caused by Alternaria solani, is a major disease in tomato cultivation, severely impacting both yield and quality. Traditional control methods rely primarily on chemical pesticides, but long-term use can lead to pathogen resistance, environmental pollution, and food safety issues. Therefore, the development of environmentally friendly and efficient biological control methods is urgently needed in agricultural science and technology. In recent years, plant immune inducers have garnered widespread attention as a novel biological control method. By stimulating the plant's immune system, they enhance its resistance to pathogens, thereby reducing the occurrence of the disease.

[0003] However, existing plant immune inducers still have many shortcomings in terms of ingredient ratio, control effect and preparation process, such as single ingredients, unstable control effect and complex preparation process. Summary of the Invention

[0004] The purpose of the present invention is to provide a natural inducer for enhancing plant immunity to resist tomato early blight and a preparation process thereof in order to solve the above-mentioned problems.

[0005] The technical solution adopted by the present invention is as follows: a natural inducer for enhancing plant immunity to resist tomato early blight and a preparation process thereof, comprising: potassium glutamate: 8-12 parts by weight; S-allyl-L-cysteine: 4-6 parts by weight; 2-piperidinic acid: 1.5-2.5 parts by weight; glutathione: 4-6 parts by weight; fulvic acid: 2.5-3.5 parts by weight; natural seaweed extract: 0.5-1.5 parts by weight; licorice extract: 1.5-2.5 parts by weight; aloe extract: 0.5-1.5 parts by weight; betaine: 0.5-1.5 parts by weight; mannitol: 0.5-1.5 parts by weight; seaweed polysaccharide: 0.5-1.5 parts by weight; glutamic acid: 0.5-1.5 parts by weight; and water: 70-80 parts by weight.

[0006] In a preferred embodiment, the preparation process comprises the following steps:

[0007] S1: Accurately weigh all raw materials according to the recipe, including potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, glutathione, fulvic acid, natural seaweed extract, licorice extract, aloe extract, betaine, mannitol, seaweed polysaccharide, glutamic acid and water;

[0008] S2: gradually add solid ingredients such as potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, glutathione, betaine, mannitol and glutamic acid to part of the water and stir until completely dissolved;

[0009] S3: Slowly add fulvic acid to the above solution while stirring to ensure thorough mixing;

[0010] S4: adding natural seaweed extract, licorice extract and aloe extract to the solution in sequence and stirring evenly;

[0011] S5: Add seaweed polysaccharide to the solution and continue stirring until it is completely dispersed;

[0012] S6: Use an appropriate amount of acid or base to adjust the pH value of the solution to an appropriate range to ensure the stability and effectiveness of each component;

[0013] S7: Add the remaining water to the solution and stir evenly to make the total water volume meet the formula requirements;

[0014] S8: homogenizing the solution, such as using a high-pressure homogenizer or ultrasonic treatment, to ensure uniformity and stability of the solution;

[0015] S9: The homogenized solution is filtered through a filter to remove any insoluble matter or impurities. The filtered clear solution is poured into a pre-sterilized container and sealed for storage. This completes the entire process for preparing a natural inducer for enhancing plant immunity against tomato early blight.

[0016] In a preferred embodiment, in step S1, the liquid ingredients such as natural seaweed extract, licorice extract and aloe extract should be stirred in advance to ensure their uniformity. All raw materials should be prepared at room temperature (20-25°C) to avoid excessively high or low temperatures that affect the properties of the raw materials.

[0017] In a preferred embodiment, in step S2, a magnetic stirrer or mechanical stirrer is used to stir the mixture at 500-600 rpm for 30 minutes until all solid components are completely dissolved and the solution becomes transparent. During the dissolution process, the water temperature should be controlled at 30-40°C to accelerate dissolution and prevent degradation of the components.

[0018] In a preferred embodiment, in step S3, the stirring speed is maintained at 300-400 rpm. The addition rate of fulvic acid should be controlled at 5-10 grams per minute to ensure thorough mixing and avoid localized excessive concentrations. During the addition process, the pH of the solution may drop and should be monitored and adjusted in real time to maintain the pH within the range of 5.0-6.0. The stirring time is continued for 15-20 minutes until the solution is uniform.

[0019] In a preferred embodiment, in step S4, after adding each extract, stir at 200-300 rpm for 5 minutes before adding the next extract. During the addition of the extracts, the solution temperature should be maintained at 25-30°C to avoid inactivation of the active ingredients due to excessive temperature. After all extracts have been added, continue stirring for 20-30 minutes to ensure thorough mixing of the ingredients.

[0020] In a preferred embodiment, in step S5, the stirring speed is controlled at 200-300 rpm. The addition rate of seaweed polysaccharide should be controlled at 2-3 grams per minute to prevent caking. During the addition process, the viscosity of the solution may increase, and the stirring speed needs to be adjusted appropriately to ensure a uniform solution. The stirring time continues for 20-30 minutes until the seaweed polysaccharide is completely dispersed and the solution is uniformly viscous.

[0021] In a preferred embodiment, in step S6, during the adjustment process, the amount of acid or base solution added each time should be controlled to be 0.5-1 ml, and stirring should be maintained at 100-200 rpm. After the pH value is adjusted, stirring is continued for 10-15 minutes to ensure that the pH value of the solution is stable.

[0022] In a preferred embodiment, in step S7, the stirring speed is controlled at 100-200 rpm. Water should be added slowly to avoid generating large bubbles. After the total water volume reaches the required amount, stirring is continued for 10-15 minutes to ensure uniformity and stability of the solution. The final volume of the solution should be precisely controlled based on actual needs, with an error within ±1%.

[0023] In step S8, a high-pressure homogenizer is used at a pressure of 50-100 MPa for 5-10 minutes. Alternatively, an ultrasonic processor is used at a power of 200-300 W for 10-15 minutes. During homogenization, the solution temperature should be maintained at 30-40°C to avoid excessive temperatures that could affect the activity of the components. After homogenization, the solution should be a smooth, uniform emulsion.

[0024] In a preferred embodiment, in step S9, during the filtration process, the pressure should be controlled at 0.1-0.2 MPa to avoid damage to the filter membrane due to excessive pressure. The filtered clear liquid should be transparent and free of suspended matter. The filtered clear liquid is filled into pre-sterilized brown glass bottles or plastic bottles at a filling speed of 500-1000 ml per minute to avoid bubbles. After filling is completed, the bottles are immediately sealed and stored to avoid contact with air. The final product should be stored in a cool and dry place with a temperature controlled at 10-20°C and a humidity controlled at 40-60%, avoiding direct sunlight and high temperatures.

[0025] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0026] 1. In the present invention, the intrinsic immune mechanism of tomato plants is effectively stimulated by scientifically proportioning key ingredients such as potassium glutamate, S-allyl-L-cysteine and 2-piperidinic acid. Potassium glutamate, as an amino acid salt, not only provides the nutrients required for plant growth, but also improves the plant's resistance to pathogens by promoting cell division and enhancing cell wall strength. S-allyl-L-cysteine, as a biologically active substance, can induce plants to produce disease-resistant enzymes such as chitinase and β-1,3-glucanase, which can decompose the cell walls of pathogens, thereby inhibiting the infection and spread of pathogens. 2-Piperidinic acid further strengthens the plant's immune response by regulating the hormone balance in the plant body, enabling tomato plants to respond quickly when faced with early blight, reducing the occurrence and spread of the disease.

[0027] 2. In the present invention, fulvic acid, natural seaweed extract, licorice extract and aloe extract are rich in a variety of bioactive ingredients, including growth hormones, trace elements and antioxidants. These ingredients can significantly improve the disease resistance and stress resistance of tomato plants. As an organic acid, fulvic acid can improve soil structure and increase soil microbial activity, thereby providing a better growth environment for plant roots and enhancing the resistance of plants to diseases. Ingredients such as polysaccharides and saponins in natural seaweed extract and licorice extract can improve the stability of plant cell membranes and enhance the tolerance of plants to adversities such as drought and salinity. The polysaccharides and flavonoids in the aloe extract have antioxidant effects, which can scavenge free radicals in plants and reduce the oxidative damage to plants caused by diseases and adversity, thereby comprehensively improving the health status and disease resistance of tomato plants.

[0028] 3. In the present invention, not only attention is paid to improving the immunity and disease resistance of tomato plants, but also consideration is given to promoting plant growth and increasing yield. Potassium glutamate, as a nitrogen source, can promote photosynthesis and protein synthesis in plants, thereby accelerating the growth and development of plants. Ingredients such as S-allyl-L-cysteine and 2-piperidinic acid can promote the growth and expansion of plant roots and improve the plant's ability to absorb water and nutrients. In addition, the addition of seaweed polysaccharides further enhances the strength and toughness of plant cell walls, which is beneficial to the division and elongation of plant cells. While enhancing immunity and disease resistance, these ingredients work together to promote the healthy growth of tomato plants and improve the photosynthetic efficiency of leaves, which ultimately manifests as a significant improvement in the yield and quality of the fruit. After using this inducer, tomato plants grow more vigorously, the fruits are fuller and more colorful, and the yield and economic benefits are significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the process principle of the present invention. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0031] Reference Figure 1 ,

[0032] Example:

[0033] A natural inducer for enhancing plant immunity to resist tomato early blight and its preparation process, characterized by comprising:

[0034] Potassium glutamate: 12 parts by weight; S-allyl-L-cysteine: 6 parts by weight; 2-piperidinic acid: 1.5 parts by weight; glutathione: 4 parts by weight; fulvic acid: 2.5 parts by weight; natural seaweed extract: 0.5 parts by weight; licorice extract: 2.5 parts by weight; aloe extract: 1.5 parts by weight; betaine: 1.5 parts by weight; mannitol: 1.5 parts by weight; seaweed polysaccharide: 1.5 parts by weight; glutamic acid: 1.5 parts by weight; water: 80 parts by weight.

[0035] The preparation process includes the following steps:

[0036] S1: Accurately weigh all raw materials according to the formula, including potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, glutathione, fulvic acid, natural seaweed extract, licorice extract, aloe extract, betaine, mannitol, seaweed polysaccharide, glutamic acid and water

[0037] S2: gradually add solid ingredients such as potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, glutathione, betaine, mannitol and glutamic acid to part of the water and stir until completely dissolved.

[0038] S3: Slowly add fulvic acid to the above solution, stirring while adding to ensure thorough mixing

[0039] S4: Add natural seaweed extract, licorice extract and aloe extract to the solution in sequence and stir evenly

[0040] S5: Add seaweed polysaccharide to the solution and continue stirring until it is completely dispersed

[0041] S6: Use appropriate amount of acid or base to adjust the pH value of the solution to the appropriate range (usually neutral or slightly acidic) to ensure the stability and effectiveness of each component

[0042] S7: Add the remaining water to the solution and stir evenly to make the total water volume meet the formula requirements

[0043] S8: Homogenize the solution, such as using a high-pressure homogenizer or ultrasonic treatment, to ensure the uniformity and stability of the solution

[0044] S9: The homogenized solution is filtered through a filter to remove any insoluble matter or impurities. The filtered clear solution is poured into a pre-sterilized container and sealed for storage. This completes the entire process for preparing a natural inducer for enhancing plant immunity against tomato early blight.

[0045] In step S1, the liquid ingredients such as natural seaweed extract, licorice extract and aloe vera extract should be stirred in advance to ensure their uniformity. All raw materials should be prepared at room temperature (20-25°C) to avoid excessively high or low temperatures that may affect the performance of the raw materials.

[0046] In step S2, a magnetic stirrer or mechanical stirrer is used to stir the mixture at 500-600 rpm for 30 minutes until all solid components are completely dissolved and the solution becomes transparent. During the dissolution process, the water temperature should be controlled at 30-40°C to accelerate dissolution and prevent degradation of the components.

[0047] In step S3, maintain a stirring speed of 300-400 rpm. The addition rate of fulvic acid should be controlled at 5-10 grams per minute to ensure thorough mixing and avoid localized excessive concentrations. During the addition process, the pH of the solution may drop; it should be monitored and adjusted in real time to maintain the pH within the range of 5.0-6.0. Stir for 15-20 minutes until the solution is uniform.

[0048] In step S4, after adding each extract, stir at 200-300 rpm for 5 minutes before adding the next extract. During the addition of the extracts, the solution temperature should be maintained at 25-30°C to avoid excessive temperatures that may inactivate the active ingredients. After all extracts have been added, continue stirring for 20-30 minutes to ensure thorough mixing.

[0049] In step S5, the stirring speed is controlled at 200-300 rpm. The addition rate of seaweed polysaccharide should be controlled at 2-3 grams per minute to prevent agglomeration. During the addition process, the viscosity of the solution may increase, and the stirring speed needs to be adjusted appropriately to ensure a uniform solution. The stirring time continues for 20-30 minutes until the seaweed polysaccharide is completely dispersed and the solution is uniformly viscous.

[0050] In step S6, the amount of acid and base solution added each time should be controlled to 0.5-1 ml, and stirring should be maintained at 100-200 rpm. After the pH value is adjusted, continue stirring for 10-15 minutes to ensure that the pH value of the solution is stable.

[0051] In step S7, the stirring speed is controlled at 100-200 rpm. When adding water, pour it in slowly to avoid generating large amounts of bubbles. Once the total water volume reaches the required amount, continue stirring for 10-15 minutes to ensure the uniformity and stability of the solution. The final volume of the solution should be precisely controlled according to actual needs, with an error within ±1%.

[0052] In step S8, use a high-pressure homogenizer at a pressure of 50-100 MPa for 5-10 minutes. Alternatively, use an ultrasonic processor at a power of 200-300 W for 10-15 minutes. During homogenization, the solution temperature should be maintained at 30-40°C to avoid excessive temperatures that could affect the activity of the components. After homogenization, the solution should be a smooth, uniform emulsion.

[0053] In step S9, during the filtration process, the pressure should be controlled at 0.1-0.2MPa to avoid damage to the filter membrane due to excessive pressure. The filtered clear liquid should be transparent and free of suspended matter. Fill the filtered clear liquid into pre-sterilized brown glass bottles or plastic bottles, and control the filling speed to 500-1000 ml per minute to avoid bubbles. After filling is completed, seal and store immediately to avoid contact with air. The final product should be stored in a cool and dry place with a temperature controlled at 10-20°C and a humidity controlled at 40% to 60%, avoiding direct sunlight and high temperature.

[0054] Test example:

[0055] Experimental Example: Evaluating the Control and Growth Promotion Effects of Natural Inducers on Tomato Early Blight I. Experimental Purpose

[0056] Verify the beneficial effects of this natural inducer in enhancing the immunity of tomato plants, resisting early blight, promoting growth and increasing yield.

[0057] 2. Test Materials

[0058] Tomato Varieties: Choose tomato varieties that are common in your area and susceptible to early blight.

[0059] Natural inducer: a natural inducer for enhancing plant immunity prepared according to the above formula.

[0060] Control group: conventional pesticide treatment group and blank control group.

[0061] Experimental fields: experimental fields with similar soil texture and fertility were selected and divided into three plots, one for inducer treatment, the other for conventional pesticide treatment and blank control.

[0062] 3. Test methods

[0063] Experimental design

[0064] The experiment was conducted with two treatments, three replicates per treatment, and six plots totaling 20 m2 each. The plots were randomly arranged in blocks. (CK treatment, SAC spraying treatment)

[0065] A backpack-mounted, automatically adjustable electric sprayer with a single conical spray head and an operating pressure of 2 kg / cm² was used for uniform spraying. No other fungicides were used during the test. A second application was conducted 7 days after the first.

[0066] The pathogen population was surveyed before application, and again 7 days after the first application, 7 days after the second application, and 14 days after the second application, for a total of three surveys. Five diagonal sampling points were used in each plot, with three plants at each point. The disease severity of all leaves was surveyed, and the disease index and control efficacy were calculated. The grading criteria are as follows:

[0067] The grading standards are as follows:

[0068] Level 0: disease-free leaves;

[0069] Level 1: There are individual small spots on one leaf;

[0070] Level 3: Less than 1 / 3 of the leaves have lesions;

[0071] Level 5: 1 / 3 to 1 / 2 of the leaves have lesions;

[0072] Level 7: All leaves have lesions;

[0073] Level 9: All leaves are rotten.

[0074] Calculation formula:

[0075] Disease index = ∑(number of diseased leaves at each level × relative value) / (total number of leaves surveyed × 9) × 100

[0076] Control effect (%) = [1 - (disease index of control area before application × disease index of treated area after application) / (disease index of control area after application × disease index of treated area before application)] × 100. IV. Data Analysis

[0077] Statistical analysis software was used to process and analyze the experimental data and compare the significance of differences among the groups.

[0078] The experimental results are shown in the table below:

[0079] 1. Disease index

[0080] Tomato Early Blight Trial Results

[0081]

[0082] 2. Growth indicators

[0083] Treatment group Plant height (cm) Stem diameter (mm) Number of blades Plant height protection index (%) Inducer-treated group 150±5 12.5±0.5 30±2 15.38 Thiophanate 600 dilution treatment group 140±6 11.0±0.6 28±3 7.69 Blank control group 130±7 10.0±0.7 25±4 -

[0084] 3. Production indicators

[0085] Treatment group Single fruit weight (g) Total yield (kg / mu) Inducer-treated group 200±10 5000±200 Thiophanate 600 dilution treatment group 180±12 4500±250 Blank control group 160±15 4000±300

[0086] Note: Data are mean ± standard deviation.

[0087] VI. Conclusion: This trial and the experimental data provided clearly demonstrate that the inducer-treated group significantly outperformed both the conventional pesticide chlorothalonil 600 dilution-treated group and the blank control group in terms of disease index, growth indicators, and yield. This fully demonstrates the significant beneficial effects of this natural inducer in enhancing tomato plant immunity, resisting early blight, promoting growth, and increasing yield, providing a strong scientific basis for its widespread application.

[0088] From the above we can know:

[0089] In the present invention, the intrinsic immune mechanism of tomato plants is effectively stimulated by scientifically proportioning key ingredients such as potassium glutamate, S-allyl-L-cysteine and 2-piperidinic acid. As an amino acid salt, potassium glutamate not only provides the nutrients required for plant growth, but also improves the plant's resistance to pathogens by promoting cell division and enhancing cell wall strength. S-allyl-L-cysteine, as a biologically active substance, can induce plants to produce disease-resistant enzymes such as chitinase and β-1,3-glucanase, which can decompose the cell walls of pathogens, thereby inhibiting the infection and spread of pathogens. 2-Piperidinic acid further strengthens the plant's immune response by regulating the hormone balance in the plant body, enabling tomato plants to respond quickly when faced with early blight, reducing the occurrence and spread of the disease.

[0090] In the present invention, various natural extracts, such as fulvic acid, natural seaweed extract, licorice extract and aloe extract, are rich in various bioactive ingredients, including growth hormones, trace elements and antioxidants. These ingredients can significantly improve the disease resistance and stress resistance of tomato plants. Fulvic acid, as an organic acid, can improve soil structure and increase soil microbial activity, thereby providing a better growth environment for plant roots and enhancing the resistance of plants to diseases. Components such as polysaccharides and saponins in natural seaweed extract and licorice extract can improve the stability of plant cell membranes and enhance the tolerance of plants to adversities such as drought and salinity. The polysaccharides and flavonoids in the aloe extract have antioxidant effects, can scavenge free radicals in plants, reduce oxidative damage to plants caused by diseases and adversity, and thus comprehensively improve the health status and disease resistance of tomato plants.

[0091] In the present invention, not only attention is paid to improving the immunity and disease resistance of tomato plants, but also consideration is given to promoting plant growth and increasing yield. Potassium glutamate, as a nitrogen source, can promote photosynthesis and protein synthesis in plants, thereby accelerating the growth and development of plants. Ingredients such as S-allyl-L-cysteine and 2-piperidinic acid can promote the growth and expansion of plant roots and improve the plant's ability to absorb water and nutrients. In addition, the addition of seaweed polysaccharides further enhances the strength and toughness of plant cell walls, which is beneficial to the division and elongation of plant cells. While enhancing immunity and disease resistance, these ingredients work together to promote the healthy growth of tomato plants and improve the photosynthetic efficiency of leaves, which ultimately manifests as a significant improvement in the yield and quality of the fruit. After using this inducer, tomato plants grow more vigorously, the fruits are fuller and more colorful, and the yield and economic benefits are significantly improved.

[0092] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply the existence of any such actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device comprising a series of elements includes not only those elements, but also other elements not explicitly listed, or elements inherent to such process, method, article, or device. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, method, article, or device comprising the element.

[0093] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A natural inducer for enhancing plant immunity against tomato early blight, characterized by: include: Potassium glutamate: 8-12 parts by weight; S-allyl-L-cysteine: 4-6 parts by weight; 2-Nipecotic acid: 1.5-2.5 parts by weight; Glutathione: 4-6 parts by weight; Fulvic acid: 2.5-3.5 parts by weight; natural seaweed extract: 0.5-1.5 parts by weight; licorice extract: 1.5-2.5 parts by weight; aloe extract: 0.5-1.5 parts by weight; betaine: 0.5-1.5 parts by weight; mannitol: 0.5-1.5 parts by weight; seaweed polysaccharide: 0.5-1.5 parts by weight; glutamic acid: 0.5-1.5 parts by weight; water: 70-80 parts by weight.

2. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: The preparation process comprises the following steps: S1: Accurately weigh all raw materials according to the recipe, including potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, glutathione, fulvic acid, natural seaweed extract, licorice extract, aloe extract, betaine, mannitol, seaweed polysaccharide, glutamic acid and water; S2: gradually add potassium glutamate, S-allyl-L-cysteine, 2-piperidinic acid, glutathione, betaine, mannitol and glutamic acid solid components to a portion of the water and stir until completely dissolved; S3: Slowly add fulvic acid to the above solution while stirring to ensure thorough mixing; S4: adding natural seaweed extract, licorice extract and aloe extract to the solution in sequence and stirring evenly; S5: Add seaweed polysaccharide to the solution and continue stirring until it is completely dispersed; S6: Use an appropriate amount of acid or base to adjust the pH value of the solution to an appropriate range to ensure the stability and effectiveness of each component; S7: Add the remaining water to the solution and stir evenly to make the total water volume meet the formula requirements; S8: homogenizing the solution, such as using a high-pressure homogenizer or ultrasonic treatment, to ensure uniformity and stability of the solution; S9: Filter the homogenized solution through a filter to remove any insoluble matter or impurities; fill the filtered clear liquid into a pre-sterilized container and seal it for storage, and then the entire preparation process of the natural inducer for enhancing plant immunity to resist tomato early blight is completed.

3. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S1, the liquid components of natural seaweed extract, licorice extract and aloe extract should be stirred in advance to ensure their uniformity; all raw materials should be prepared at room temperature of 20-25°C to avoid excessively high or low temperature affecting the performance of the raw materials.

4. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S2, a magnetic stirrer or a mechanical stirrer is used to stir the mixture at a speed of 500-600 rpm for 30 minutes until all solid components are completely dissolved and the solution becomes transparent. During the dissolution process, the water temperature should be controlled at 30-40° C. to accelerate dissolution and prevent degradation of the components.

5. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S3, the stirring speed is maintained at 300-400 rpm; the addition rate of fulvic acid should be controlled at 5-10 grams per minute to ensure sufficient mixing and avoid local excessive concentration; during the addition process, the pH value is maintained in the range of 5.0-6.0; and the stirring time is continued for 15-20 minutes until the solution is uniform.

6. The process for preparing the natural inducer for enhancing plant immunity against tomato early blight according to claim 1, characterized in that: In step S4, after each extract is added, the mixture is stirred at a speed of 200-300 rpm for 5 minutes before the next extract is added. During the addition of the extracts, the solution temperature should be maintained at 25-30°C to avoid inactivation of the active ingredients due to excessive temperature. After all extracts are added, stirring is continued for 20-30 minutes to ensure that all ingredients are fully mixed.

7. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S5, the stirring speed is controlled at 200-300 rpm; the addition rate of seaweed polysaccharide should be controlled at 2-3 grams per minute to prevent agglomeration; during the addition process, the viscosity of the solution may increase, and the stirring speed needs to be appropriately adjusted to ensure a uniform solution; the stirring time is continued for 20-30 minutes until the seaweed polysaccharide is completely dispersed and the solution becomes uniformly viscous.

8. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S6, during the adjustment process, the amount of acid and base solution added each time should be controlled at 0.5-1 ml, and stirring should be maintained while adding, and the stirring speed should be maintained at 100-200 rpm; after the pH value adjustment is completed, stirring is continued for 10-15 minutes to ensure that the pH value of the solution is stable.

9. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S7, the stirring speed is controlled at 100-200 rpm; when adding water, it should be poured slowly to avoid generating a large number of bubbles; after the total water volume reaches the formula requirements, stirring is continued for 10-15 minutes to ensure the uniformity and stability of the solution; the volume of the final solution should be accurately controlled according to actual needs, with an error within ±1%; In step S8, a high-pressure homogenizer is used with a pressure controlled at 50-100 MPa and a treatment time of 5-10 minutes; or an ultrasonic processor is used with a power controlled at 200-300 W and a treatment time of 10-15 minutes; during the homogenization process, the solution temperature should be controlled at 30-40°C to avoid excessive temperature affecting the activity of the components; after homogenization, the solution should appear as a delicate and uniform emulsion.

10. The process for preparing the natural inducer for enhancing plant immunity to resist tomato early blight according to claim 1, characterized in that: In step S9, during the filtration process, the pressure should be controlled at 0.1-0.2 MPa to avoid damage to the filter membrane due to excessive pressure; the filtered clear liquid should be transparent and free of suspended matter; the filtered clear liquid is filled into pre-sterilized brown glass bottles or plastic bottles at a filling speed of 500-1000 ml per minute to avoid bubbles; after filling, the bottles are immediately sealed and stored to avoid contact with air; the final product should be stored in a cool and dry place at a temperature of 10-20°C and a humidity of 40-60%, avoiding direct sunlight and high temperatures.

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