Tea saponin composition with anti-oxidation and whitening effects and application thereof

Abstract

The invention relates to a tea saponin composition with anti-oxidation and whitening effects and application of the tea saponin composition, and belongs to the technical field of cosmetics. The tea saponin composition is prepared from the following components in parts by mass: 3 to 6 parts of tea saponin liquid, 0.5 to 2 parts of roxburgh rose fruit extract and 1 to 3 parts of radix saposhnikoviae extract. The tea saponin provided by the invention realizes improvement of skin feeling and efficacy, improves safety, and can also be used as an emulsifier. By optimizing the process of using tea saponin in cosmetics, other impurity components are reduced. The rosa roxburghii tratt fruit extract is high in antioxidant and whitening activity, and irritation to skin is reduced while the effect of the rosa roxburghii tratt fruit extract is guaranteed. The content of coumarin compounds and furocoumarin structural substances in the radix saposhnikoviae root extract is higher than that of coumarin compounds and furocoumarin structural substances in the radix saposhnikoviae stem extract, and the radix saposhnikoviae root extract has higher light protection and anti-inflammatory activity and is more suitable for forming a moisturizing film. The cosmetic prepared from the tea saponin composition and the preparation method thereof is higher in stability, good in antioxidant and whitening effects and higher in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a tea saponin composition with antioxidant and whitening effects and application thereof. Background Art

[0002] Tea saponins are triterpenoid saponin compounds extracted from plants of the Theaceae family (such as tea leaves and camellia seeds). They possess surface-active, antibacterial, anti-inflammatory, and antioxidant properties. They are commonly found in cleaning products as natural, mild surfactants used in facial cleansers, shampoos, and more. Tea saponins can be used as natural emulsifiers, but their complex composition can adversely affect the stability, color, and odor of the formula. Furthermore, plant-based emulsifiers have low levels of active ingredients and are susceptible to degradation or failure under varying environmental conditions (such as temperature and pH changes), impacting the shelf life of cosmetics.

[0003] Rosa roxburghii fruit extract is rich in vitamin C, with an L-ascorbic acid content of 2000mg / 100g, over 100 times that of lemon. It offers facial benefits such as soothing, sun protection, anti-aging, and whitening. However, since Rosa roxburghii fruit extract requires an acidic environment (pH ≤ 4) to remain active, it may irritate sensitive skin. Therefore, when used in cosmetics, care must be taken to ensure both its effectiveness and irritation levels are strictly controlled.

[0004] Saposhnikovia root is rich in coumarins (such as psoralen), volatile oils, and polysaccharides, which have anti-allergic, anti-inflammatory, and immunomodulatory effects. Its extracts can be used in skin care products and cosmetics for soothing, anti-aging, whitening, sensitive skin, and anti-hair loss.

[0005] Currently, there is a need to further improve the safety and stability of cosmetics by adjusting the formula and preparation method without affecting the efficacy of the various components of the cosmetics. Summary of the Invention

[0006] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a tea saponin composition with antioxidant and whitening effects and its application.

[0007] To achieve the above object, the technical solution adopted by the present invention is:

[0008] In a first aspect, the present invention provides a tea saponin composition comprising the following components in parts by weight: 3 to 6 parts of tea saponin, 0.5 to 2 parts of roxburghii fruit extract, and 1 to 3 parts of siler root extract.

[0009] By selecting a specific component ratio, the tea saponin composition obtained by the present invention has antioxidant and whitening effects, making the formula more stable and safe.

[0010] Tea saponin is a natural surfactant with amphiphilic properties. It can reduce the content of synthetic emulsifiers used in cream cosmetics, not only improving skin feel and efficacy, but also improving safety.

[0011] Compared to the stem, the roots of Saposhnikovia divaricata are rich in coumarin compounds, with their content 3-5 times higher than in the stems and leaves. They also possess a unique furanocoumarin structure (such as psoralen), which gives them enhanced photoprotection and anti-inflammatory activity. The molecular weight distribution of Saposhnikovia divaricata polysaccharides (10-50 kDa) is more suitable for forming a moisturizing film, with a moisturizing effect 1.8 times that of polysaccharides in the stems and leaves.

[0012] Furthermore, the mass ratio of the total mass of the roxburghii fruit extract and the siler root extract to the tea saponin liquid is 2:3.

[0013] As a preferred embodiment of the present invention, the mass ratio of the roxburghii fruit extract to the siler root extract is 1:3.

[0014] Most preferably, the tea saponin composition comprises the following components in parts by mass: 6 parts of tea saponin liquid, 1 part of roxburghii fruit extract and 3 parts of siler root extract.

[0015] In a specific embodiment of the present invention, the tea saponin liquid comprises tea saponin and water, and the mass percentage of tea saponin is 98%.

[0016] In a second aspect, the present invention provides the use of the tea saponin composition in the preparation of antioxidant and / or whitening products.

[0017] In a third aspect, the present invention provides a cosmetic comprising the tea saponin composition.

[0018] Furthermore, it also includes cosmetic accessories.

[0019] In a specific embodiment of the present invention, the cosmetic excipient contains at least one of glycerin, caprylic / capric triglyceride, jojoba oil, tocopherol, citric acid, phenoxyethanol, triethanolamine and deionized water.

[0020] In a fourth aspect, the present invention provides a method for preparing the cosmetic, comprising the following steps:

[0021] S1: Mix tea saponin with ethanol, stir and dissolve evenly at 60-70°C, filter, add water and concentrate by vacuum distillation to obtain tea saponin liquid;

[0022] S2: mixing the roxburghii fruit extract with deionized water, stirring at 60-70° C. to uniformly dissolve the mixture, and filtering to obtain an aqueous solution of the roxburghii fruit extract;

[0023] S3: caprylic / capric triglyceride, jojoba oil, and tocopherol are mixed and stirred at 60-70° C. to completely dissolve and mix to obtain an oil phase;

[0024] S4: Adjust the temperature of deionized water to 60-70° C., add glycerin, stir evenly, add the tea saponin liquid from step S1, stir evenly to obtain an aqueous phase; the tea saponin liquid serves as the main emulsifier and can help emulsify the oil and water phases.

[0025] S5: Mix the oil phase and the water phase and stir at a speed of 1000-2000 r / min for 3-5 minutes to form colostrum;

[0026] S6: adjusting the stirring speed to 500-800 r / min while cooling the colostrum; adding the radix psidium extract when the colostrum is cooled to below 40° C. and stirring evenly to obtain emulsion 1;

[0027] S6: Adjust the pH value of emulsion 1 to 4-5 with citric acid, add the roxburghii fruit extract aqueous solution of step S2, and stir evenly to obtain emulsion 2; when emulsion 2 is cooled to below 30°C, add phenoxyethanol and stir evenly to obtain emulsion 3; adjust the pH value of emulsion 3 to 5-6 with triethanolamine, let it stand, and obtain tea saponin whitening essence emulsion.

[0028] Furthermore, in step S1, tea saponin and ethanol are mixed and stirred at 100-200 r / min for 15-30 min to obtain a tea saponin ethanol mixed solution.

[0029] Furthermore, in step S1, the ratio of tea saponin to ethanol is 95-105 g:100 mL, preferably 98 g:100 mL.

[0030] Furthermore, in step S1, the tea saponin ethanol mixture is adjusted to a temperature of 60-70°C and stirred at 100-200 r / min for 1-2 hours to obtain a dissolved tea saponin ethanol mixture. Preferably, the tea saponin ethanol mixture is adjusted to a temperature of 65°C and stirred for 1.5 hours.

[0031] Furthermore, in step S1, the dissolved tea saponin ethanol mixture is filtered twice;

[0032] The first step is to filter the solution with filter paper, followed by nanofiltration with a molecular weight cutoff of 900-1000. The retentate from the nanofiltration is diluted with ethanol 1-3 times. Preferably, the molecular weight cutoff is 950 and the dilution frequency is 3. This ensures that all impurities are removed and the purity of the tea saponin is improved, thereby obtaining a tea saponin ethanol filtration mixture.

[0033] Furthermore, the volume concentration of ethanol is 75% to 100%, preferably 85%.

[0034] Furthermore, during each dilution, the volume ratio of ethanol to the retentate of the nanofiltration is 1:(15-25), preferably 1:20.

[0035] Furthermore, in step S1, when water is added, the ratio of the tea saponin ethanol filtered mixed solution to water is 1 g: 2-3 mL, preferably 1 g: 2.5 mL.

[0036] Furthermore, in step S1, during the vacuum distillation concentration process, the water bath temperature is 40-50° C., the pressure is 10-20 mmHg (about 1.3-2.7 kPa), and the distillation is performed for 1-2 hours. Preferably, the water bath temperature is 45° C., the pressure is 15 mmHg, the distillation is performed for 1.5 hours, and the mass concentration of the concentrated tea saponin liquid is 98%. The temperature of the heating bath should be strictly controlled during the preparation process to prevent the tea saponin efficacy from being damaged by high temperature. The tea saponin liquid is sealed and stored in a cool, dry place to avoid light and high temperature.

[0037] Furthermore, in step S2, the roxburghii fruit extract is mixed with deionized water in a mass ratio of 1:(40-60) to obtain a roxburghii fruit extract-water mixture. Preferably, the mass ratio of the roxburghii fruit extract to deionized water is 1:50.

[0038] Furthermore, in step S2, the mixture is stirred and dissolved uniformly at 100-200 r / min and 65° C. for 1-2 h to ensure that the roxburghii fruit extract is completely dissolved to obtain a roxburghii fruit extract liquid. Preferably, the mixture is stirred at 200 r / min for 1.5 h.

[0039] Furthermore, in step S3, the temperature is 65°C.

[0040] Furthermore, in step S4, the temperature is 65°C.

[0041] Furthermore, in step S5, stirring is performed at a speed of 1500 r / min for 4 minutes.

[0042] Furthermore, in step S6, the stirring speed is adjusted to 500 r / min to prevent emulsion stratification.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] The present invention uses tea saponin as an active ingredient, which not only improves the skin feel and efficacy, but also improves the safety of cosmetics. At the same time, it can also be used as an emulsifier to reduce the irritation caused by the use of other chemically synthesized emulsifiers. By optimizing the process of using tea saponin in cosmetics, the stability problems caused by other impurity components are reduced. The roxburghii fruit extract of the present invention has high antioxidant and whitening activities. By adjusting the pH value during the preparation of cosmetics, while ensuring the efficacy of the roxburghii fruit extract, the irritation to the skin is reduced. The radix siler root extract of the present invention has a higher content of coumarin compounds and furanocoumarin structural substances than the radix siler stem extract, and has stronger light protection and anti-inflammatory activity. The molecular weight distribution range of the polysaccharide in the radix siler root is more suitable for forming a moisturizing film, and the moisturizing effect is 1.8 times that of the polysaccharide in the stem and leaf. The cosmetics prepared by the tea saponin composition of the present invention and the preparation method thereof have higher stability, good antioxidant and whitening effects, and higher safety. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 This is the antioxidant test result diagram of the test example;

[0046] Figure 2 This is a graph showing the tyrosinase inhibition test results of the test example. DETAILED DESCRIPTION

[0047] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to specific examples. Other materials, reagents, etc. used in the examples, unless otherwise specified, can be obtained from commercial sources.

[0048] Tea saponin (purity 98%, mass percentage), brown powder, was purchased from Xi'an Hongtaiyuan Biotechnology Co., Ltd.

[0049] Rosa roxburghii fruit extract, as a powder, was purchased from Shanxi Zhongnuo Biotechnology Co., Ltd.

[0050] The extract of radix fangfeng was purchased from Guangzhou Hengyue Biotechnology Co., Ltd.

[0051] The extract of siler stem was purchased from Guangzhou Hengyue Biotechnology Co., Ltd.

[0052] Citrus aurantium extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0053] Examples 1 to 3 and Comparative Examples 1 to 10

[0054] The components (parts by mass) of the tea saponin compositions of Examples 1 to 3 and Comparative Examples 1 to 10 are shown in Tables 1 and 2.

[0055] Table 1

[0056] Components Example 1 Example 2 Example 3 Refined tea saponin liquid 6 3 6 Rosa roxburghii fruit extract 1 0.5 2 Saposhnikovia root extract 3 1 3

[0057] Table 2

[0058]

[0059]

[0060] Application Examples 1 to 3 and Comparative Application Examples 1 to 10

[0061] Tea saponin whitening essence lotion was prepared according to the tea saponin compositions of Examples 1 to 3 and Comparative Examples 1 to 10.

[0062] In each application example, the mass percentage of the tea saponin composition in the tea saponin whitening essence is shown in Table 3.

[0063] Table 3

[0064] Application Examples Tea saponin composition Addition amount of tea saponin composition 1 Example 1 10％ 2 Example 2 4.5％ 3 Example 3 11％

[0065] In each comparative application example, the mass percentage of the tea saponin composition in the tea saponin whitening essence lotion is shown in Table 4.

[0066] Table 4

[0067] Application comparison Tea saponin composition Addition amount of tea saponin composition 1 Comparative Example 1 10％ 2 Comparative Example 2 10％ 3 Comparative Example 3 10％ 4 Comparative Example 4 10％ 5 Comparative Example 5 10％ 6 Comparative Example 6 10％ 7 Comparative Example 7 10％ 8 Comparative Example 8 10％ 9 Comparative Example 9 10％

[0068] The formula of tea saponin whitening essence is shown in Table 5.

[0069] Table 5

[0070]

[0071]

[0072] The preparation method of tea saponin whitening essence lotion is as follows:

[0073] 1. Preparation of tea saponin liquid:

[0074] (1) After purchasing 98% pure tea saponin solid powder, select ethanol as the solvent. Weigh 98g of tea saponin solid and 100mL of ethanol, stir at 150r / min for 30min, and stir evenly to obtain a tea saponin ethanol mixture. Place the tea saponin ethanol mixture in a water bath and heat it to 65℃. Maintain this temperature and stir and dissolve for 1-2h to obtain a dissolved tea saponin ethanol mixture.

[0075] (2) Filter the dissolved tea saponin ethanol mixture with filter paper to remove undissolved solid impurities and obtain the first filtered liquid; use a nanofiltration membrane with a molecular weight cutoff of 950 to perform a second nanofiltration separation on the first filtered liquid. During the nanofiltration process, 85% (v / v) ethanol is added to dilute the retained liquid for 3 times. The volume ratio of ethanol to the retained liquid is 1:20 for each dilution to ensure that impurities are removed and the purity of tea saponin is improved to obtain the tea saponin ethanol filtered mixture.

[0076] (3) Add 100g of tea saponin ethanol filtered mixed solution to 250mL of deionized water, place it in a vacuum distillation apparatus, set the water bath temperature of the rotary evaporator to 45°C and the pressure to 15mmHg, and distill for 1.5h to obtain tea saponin vacuum distillate. After the ethanol is completely evaporated, the tea saponin vacuum distillate is composed of tea saponin and water, and then continue to reduce pressure and concentrate until the concentration of tea saponin vacuum distillate is 98% (mass percentage) to obtain tea saponin liquid. The temperature of the heating bath should be strictly controlled during the preparation process to avoid the high temperature damage to the efficacy of tea saponin. The tea saponin liquid should be sealed and stored in a cool, dry place, away from light and high temperature.

[0077] 2. Processing and dissolving pear fruit extract

[0078] (1) Pretreatment of the roxburghii fruit extract: adding the roxburghii fruit extract powder to deionized water at a mass ratio of 1:50, stirring evenly to obtain a roxburghii fruit extract-water mixture.

[0079] (2) Heating and dissolving: The mixed solution of the roxburghii fruit extract and water was placed in a water bath and heated to 65° C., and the mixture was stirred at 200 r / min to dissolve for 1.5 h to ensure that the roxburghii fruit extract was completely dissolved to obtain a roxburghii fruit extract liquid.

[0080] (3) Filtration: Filter the roxburghii fruit extract liquid with filter paper to remove undissolved solid impurities to obtain a roxburghii fruit extract aqueous solution.

[0081] 3. Prepare the oil phase: mix caprylic / capric triglyceride, jojoba oil and tocopherol, heat to 65°C, stir evenly to completely dissolve and mix, and obtain the oil phase.

[0082] 4. Prepare the aqueous phase: Add deionized water to the main mixing vessel and heat to 65°C; add glycerin and stir evenly; add the tea saponin liquid to the aqueous phase and stir evenly to obtain the aqueous phase. Tea saponin liquid acts as the main emulsifier, helping to emulsify the oil and water phases.

[0083] 5. Emulsification: Slowly add the oil phase into the water phase and use a homogenizer to stir at a speed of 1500r / min for 4 minutes to form colostrum.

[0084] 6. Cool the emulsion: Place the colostrum in a cooling water bath and slowly cool it to room temperature. Reduce the stirring speed to 600r / min to prevent emulsion stratification.

[0085] 7. Adding the radix fangfeng extract liquid: When the colostrum is cooled to below 40°C, slowly add the radix fangfeng extract and stir evenly to obtain emulsion 1.

[0086] 8. First pH adjustment: adjust the pH of the emulsion containing the radix psidium extract to 4-5 with a citric acid solution, and add an aqueous solution of the roxburghii fruit extract to obtain emulsion 2.

[0087] 9. Adding preservative: When emulsion 2 is cooled to below 30°C, add preservative (phenoxyethanol) and stir evenly to obtain emulsion 3.

[0088] 10. Second pH Adjustment: Test the pH of Lotion 3 and adjust it to 5-6 with triethanolamine. Return to neutral; otherwise, skin irritation may occur. Let stand to obtain the Tea Saponin Whitening Essence Lotion.

[0089] Comparative Application Example 11

[0090] In the application example 1, when preparing the tea saponin liquid, nanofiltration was not performed, and other preparation methods remained unchanged to prepare the tea saponin whitening essence lotion.

[0091] Application Comparative Example 12

[0092] In the application example 1, when preparing the tea saponin liquid, the ethanol dilution times is 1 time, and other preparation methods remain unchanged to prepare the tea saponin whitening essence lotion.

[0093] Comparative Application Example 13

[0094] In the application example 1, the pH value of the emulsion 1 is not adjusted in step 8, and the roxburghii fruit extract aqueous solution is directly added. The other preparation methods remain unchanged to prepare the tea saponin whitening essence lotion.

[0095] Application Comparative Example 14

[0096] In the application example 1, the pH value is not adjusted in step 10, and the other preparation methods remain unchanged to prepare the tea saponin whitening essence lotion.

[0097] Test Example 1 Antioxidant

[0098] 1. Reagents and Materials

[0099] (1) Reagents: 0.2 mM DPPH solution (1,1-diphenyl-2-trinitrophenylhydrazine, C 18 H 12N5O6): Weigh 0.007875 g of DPPH powder and dissolve it in 100 mL of anhydrous ethanol to prepare 0.2 mM DPPH;

[0100] VC solution (vitamin C, positive control group): dilute VC with deionized water to 0.001 mg / mL, 0.002 mg / mL, 0.004 mg / mL, 0.006 mg / mL, 0.008 mg / mL, 0.01 mg / mL, 0.02 mg / mL and 0.5 mg / mL, and place in a brown bottle for later use.

[0101] (2) Instruments and equipment: analytical balance (accurate to 0.001 g), ultrasonic cleaning machine, and UV-visible spectrophotometer.

[0102] 2. Test steps

[0103] Divide into 4 sample tubes and add samples in sequence according to Table 6.

[0104] After mixing the solutions in each tube, react them at room temperature in the dark for 30 minutes, adjust the wavelength to zero with distilled water at 517 nm, measure the absorbance with a spectrophotometer, and record the data (in data processing, it should be noted that the absolute difference between two independent measurement results obtained under repeatability conditions should not exceed 10% of the arithmetic mean).

[0105] The clearance rate (P) was calculated according to formula (1); the test results are shown in Table 7 and Figure 1 shown.

[0106] P = [1 - (T - T0) / (C - C0)] × 100% - Formula (1)

[0107] In formula (1), T is the absorbance of the sample tube, that is, the absorbance of the solution after the test sample reacts with DPPH; T0 is the background absorbance of the test sample; C is the absorbance of the DPPH tube, that is, the absorbance of the DPPH solution when no test sample is added; C0 is the background absorbance of the solvent (anhydrous ethanol).

[0108] Table 6 Reaction solution composition

[0109] Reagents T-sample tube T0-sample blank tube C—Control tube C0—control blank Test sample (mL) 2 2 / / Anhydrous ethanol (mL) / 2 2 4 DPPH solution (mL) 2 / 2 /

[0110] Table 7 Antioxidant test results

[0111]

[0112]

[0113] Test Example 2: Stability

[0114] 1. Heat resistance test: The tea saponin whitening essence milk samples prepared in Examples 1 to 3 and Comparative Examples 1 to 13 were placed in an electric constant temperature incubator and the temperature was adjusted to 40±1°C. The samples were taken out on the 1st, 7th and 45th days and allowed to return to room temperature to observe whether there were any abnormal phenomena such as discoloration, delamination, precipitation, or taste change.

[0115] 2. Cold resistance test: The tea saponin whitening essence milk samples prepared in Examples 1 to 3 and Comparative Examples 1 to 13 were placed in a constant temperature refrigerator and the temperature was adjusted to -15±1°C. The samples were taken out on the 1st, 7th and 45th days and allowed to return to room temperature to observe whether there were any abnormal phenomena such as discoloration, delamination, precipitation, or taste change.

[0116] The results are shown in Table 8.

[0117] Table 8 Stability test

[0118]

[0119]

[0120] Test Example 3: Human Safety Questionnaire Test

[0121] 1. Experimental Methods

[0122] 90 volunteers aged 20 to 45 were selected and divided into 18 groups of 5 people each. All of them were female and had skin tightening needs. The tea saponin whitening essence lotion prepared in Examples 1 to 3 and Comparative Examples 1 to 14 was used, and a bisabolol lotion was added for comparison. The bisabolol lotion was prepared by replacing the tea saponin composition in Example 1 with an equal amount of bisabolol. The lotion was applied once daily, morning and evening, after cleansing, for 4 consecutive weeks. The patients were surveyed on product irritation at week 2 and week 4 using questionnaires (Table 9).

[0123] Table 9

[0124] Irritation Evaluation Criteria Obvious irritation Stinging, noticeable redness, peeling, and dryness More stimulating There is a stinging sensation, noticeable redness, slight peeling, and dryness milder Slight tingling sensation, no peeling, no redness, and no obvious dryness mild The product is comfortable, non-irritating, non-redness, and non-drying

[0125] 2. Experimental Results

[0126] As shown in Table 10, the tea saponin whitening essence lotions prepared in Application Examples 1 to 3 of the present invention are milder and less irritating to the skin than those in other application comparison examples.

[0127] Table 10

[0128]

[0129]

[0130] Test Example 4: Whitening Cell Efficacy Experiment

[0131] 1. Reagents and Materials

[0132] (1) Reagents: tyrosinase (25 kU), levodopa (purity ≥ 98%).

[0133] (2) Reagent preparation:

[0134] Prepare 0.05 mol / L PBS (pH = 6.8) buffer: Solution A: Weigh 7.099 g of disodium hydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L Na2HPO4 solution; Solution B: Weigh 6.803 g of potassium dihydrogen phosphate and add distilled water to 1000 mL to obtain a 0.05 mol / L KH2PO4 solution; Mix 50 mL of Solution A and 50 mL of Solution B to obtain 0.05 mol / L PBS (pH = 6.8).

[0135] Preparation of tyrosinase solution: Use 0.05 mol / L PBS (pH=6.8) buffer to prepare tyrosinase to 100 U / mL and prepare it immediately before use.

[0136] Prepare levodopa solution: weigh 0.04 g levodopa, dissolve in 40 mL 0.05 mol / L PBS (pH = 6.8) buffer, and store in the dark.

[0137] 2. Instruments and equipment: analytical balance (accurate to 0.0001 g), 1 L volumetric flask, 50 mL graduated cylinder, 1000 μL pipette, constant temperature water bath and UV-visible spectrophotometer.

[0138] 3. Measurement steps

[0139] Prepare the test solution according to Table 11:

[0140] The test sample (tea saponin whitening essence), 0.05M PBS buffer (pH 6.8), and tyrosinase solution were sequentially added to the test tube.

[0141] Table 11 Reaction solution composition (mL)

[0142]

[0143] After preparing the test solution, place the test tube in a 37°C water bath for 10 minutes; then, add 2 mL of levodopa solution, react for 5 minutes, and measure the absorbance at 475 nm. The absolute difference between two independent measurement results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean. Calculate the tyrosinase inhibition rate (%) according to formula (2).

[0144] Tyrosinase inhibition rate (%) = [1-(ODC-ODD) / (ODA-ODB)] × 100% - Formula (2)

[0145] In the above formula, ODA is the absorbance value of the enzyme solution control group; ODB is the absorbance value of the enzyme solution control blank group; ODC is the absorbance value of the sample group; ODD is the absorbance value of the sample group blank control.

[0146] The results of tyrosinase inhibition rate are shown in Table 12 and Figure 2 .

[0147] Table 12 Tyrosinase inhibition rate test results

[0148] Group Tyrosinase inhibition (%) Group Tyrosinase inhibition (%) Application Example 1 77.4 Application Comparative Example 7 25.4 Application Example 2 71.1 Comparative Application Example 8 39.8 Application Example 3 76.3 Comparative Application Example 9 41.2 Comparative Application Example 1 17.3 Comparative Application Example 10 46.3 Application Comparative Example 2 19.3 Comparative Application Example 11 62.2 Application Comparative Example 3 12.5 Application Comparative Example 12 61.9 Comparative Application Example 4 58.9 Comparative Application Example 13 60.3 Comparative Application Example 5 60.1 Application Comparative Example 14 65.3 Application Comparative Example 6 28.9

[0149] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A tea saponin composition, characterized in that The invention comprises the following components in parts by mass: 3-6 parts of tea saponin liquid, 0.5-2 parts of roxburghii fruit extract and 1-3 parts of fangfeng root extract.

2. The tea saponin composition according to claim 1, wherein The mass ratio of the total mass of the roxburghii fruit extract and the siler root extract to the tea saponin liquid is 2:

3.

3. The tea saponin composition according to claim 2, characterized in that The mass ratio of the roxburghii fruit extract to the siler root extract is 1:

3.

4. The tea saponin composition according to claim 3, characterized in that The tea saponin composition comprises the following components in parts by mass: 6 parts of tea saponin liquid, 1 part of roxburghii fruit extract and 3 parts of siler root extract.

5. Use of the tea saponin composition according to any one of claims 1 to 4 in the preparation of antioxidant and / or whitening products.

6. A cosmetic, characterized in that: The cosmetic contains the tea saponin composition according to any one of claims 1 to 4.

7. The cosmetic according to claim 6, wherein Also includes cosmetic accessories.

8. The cosmetic according to claim 7, wherein The cosmetic auxiliary material contains at least one of glycerin, caprylic / capric triglyceride, jojoba oil, tocopherol, citric acid, phenoxyethanol, triethanolamine and deionized water.

9. The method for preparing the cosmetic according to any one of claims 6 to 8, characterized in that: The following steps are involved: S1: Mix tea saponin with ethanol, stir and dissolve evenly at 60-70°C, filter, add water and concentrate by vacuum distillation to obtain tea saponin liquid; S2: mixing the roxburghii fruit extract with deionized water, stirring at 60-70° C. to uniformly dissolve the mixture, and filtering to obtain an aqueous solution of the roxburghii fruit extract; S3: caprylic / capric triglyceride, jojoba oil, and tocopherol are mixed and stirred at 60-70° C. to completely dissolve and mix to obtain an oil phase; S4: Adjust the temperature of deionized water to 60-70°C, add glycerin, stir evenly, add the tea saponin liquid from step S1, stir evenly to obtain an aqueous phase; S5: Mix the oil phase and the water phase and stir at a speed of 1000-2000 r / min for 3-5 minutes to form colostrum; S6: adjusting the stirring speed to 500-800 r / min while cooling the colostrum; adding the radix psidium extract when the colostrum is cooled to below 40° C. and stirring evenly to obtain emulsion 1; S6: Adjust the pH value of emulsion 1 to 4-5 with citric acid, add the roxburghii fruit extract aqueous solution of step S2, and stir evenly to obtain emulsion 2; when emulsion 2 is cooled to below 30°C, add phenoxyethanol and stir evenly to obtain emulsion 3; adjust the pH value of emulsion 3 to 5-6 with triethanolamine, let it stand, and obtain tea saponin whitening essence emulsion.

10. The preparation method according to claim 9, wherein In step S1, filtering is performed twice; The first step is to filter with filter paper, and the second step is to perform nanofiltration. The molecular weight cut-off of nanofiltration is 900 to 1000. The retentate of nanofiltration is diluted with ethanol, and the dilution times are 1 to 3 times.

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