Application of haworthia cooperi polysaccharide extract in preparation of preparation for promoting skin wound healing

Abstract

The invention belongs to the technical field of biological medicines, and particularly discloses application of a haworthia cooperi polysaccharide extract in preparation of a preparation for promoting skin wound healing. The haworthia cooperi polysaccharide extract is prepared by the steps of crushing fresh haworthia cooperi leaves, performing ultrasonic extraction, performing centrifugation, performing ethanol precipitation, performing washing, performing drying and the like, and is used for preparing medicines, skin care products or dressings for promoting skin wound healing. Animal experiments show that when the product containing the haworthia cooperi polysaccharide extract is used for treating wounds, compared with a normal saline control group, the wound healing time can be remarkably shortened, and the healing rate can be increased. The haworthia cooperi polysaccharide extract is wide in source, simple in extraction method and low in cost, has good biocompatibility and safety, and provides a safe and effective new choice for skin wound healing.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and particularly relates to an application of a Gypsum Fibrosum polysaccharide extract in preparing a preparation for promoting skin wound healing. Background Art

[0002] As the largest organ in the human body, the skin serves as a vital barrier against environmental aggressors. When the skin sustains trauma, wound healing is a complex and orderly biological process involving inflammatory response, cell proliferation, and tissue remodeling. However, in clinical practice, various factors can lead to delayed wound healing, such as diabetes, infection, and aging. This not only increases patient suffering and treatment costs, but can also lead to serious complications.

[0003] Currently, numerous methods and products are available to promote skin wound healing, including traditional dressings, growth factor drugs, and antibiotics. Traditional dressings primarily protect wounds and prevent infection, but their effectiveness in promoting wound healing is limited. While growth factor drugs can effectively promote cell proliferation and differentiation, they suffer from poor stability, high cost, and the potential for immune response. Antibiotics are primarily used to prevent and treat wound infections and have little direct effect on wound healing. Therefore, the development of safe, effective, and cost-effective new substances and methods to promote skin wound healing holds significant clinical significance and market value. Summary of the Invention

[0004] The present invention aims to solve at least one of the technical problems in the related art to a certain extent. To this end, the main purpose of the present invention is to provide a use of a Gypsum Fibrosum polysaccharide extract in the preparation of a preparation for promoting skin wound healing, which has the characteristics of being safe, effective and economical.

[0005] The purpose of the present invention is achieved through the following technical solutions:

[0006] Application of Gypsum Fibrosum polysaccharide extract in preparing preparations for promoting skin wound healing.

[0007] In certain embodiments, the polysaccharide extract is obtained by the following method:

[0008] Fresh leaves of Jade Dew are taken, washed, added with an appropriate amount of deionized water, and crushed with a blender to obtain Jade Dew pulp; deionized water is added to the Jade Dew pulp at a material-liquid ratio of 1:(1-10), and ultrasonic extraction is performed at 60-80°C for 1-3 hours; after the extract is cooled, it is centrifuged at 4000-6000 r / min for 10-20 minutes, and the supernatant is collected; the supernatant is concentrated under reduced pressure at 60°C to a concentrated solution with a volume of 1 / 10 of the original solution; 3-5 times the volume of 95% ethanol is added to the concentrated solution, and the solution is allowed to stand overnight to precipitate polysaccharides; the precipitate is collected, washed with anhydrous ethanol 2-3 times, and vacuum-dried to obtain a Jade Dew polysaccharide extract.

[0009] In some specific embodiments, the jade dew is grass jade dew and its variants.

[0010] In certain embodiments, the preparation includes but is not limited to one of a medicine, a dressing, or a skin care product.

[0011] Preferably, the preparation further comprises a pharmaceutically acceptable carrier, such carriers including (but not limited to): diluents, buffers, suspensions, emulsions, granules, encapsulations, excipients, fillers, adhesives, sprays, transdermal absorbents, wetting agents, disintegrants, absorption enhancers, surfactants, colorants, flavorings or adsorption carriers.

[0012] The preparation of the present invention can be prepared into various dosage forms as needed, including but not limited to tablets, solutions, granules, patches, ointments, capsules, aerosols or suppositories.

[0013] The administration route of the preparation of the present invention is not limited, as long as it can exert the desired therapeutic or preventive effect, including but not limited to oral administration, intravenous injection, intramuscular injection, subcutaneous injection, sublingual dissolution, rectal irrigation, nasal spray, oral spray, local skin or systemic transdermal administration.

[0014] The preparation of the present invention can also be used in combination with other drugs that promote skin wound healing. The combined use of multiple drugs can greatly increase the success rate of treatment.

[0015] Compared with the prior art, the present invention has at least the following advantages:

[0016] The present invention discovers for the first time that Gyokuro polysaccharide extract significantly promotes skin wound healing. Animal experiments have shown that treating wounds with medications, skin care products, or dressings containing Gyokuro polysaccharide extract significantly shortens wound healing time and improves wound healing rates. In the experiment, compared with the control group, the wound healing time in the experimental group was shortened by an average of three days, and the healing rate increased by 45%. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the specific embodiments of the present invention, the following briefly introduces the drawings required for the specific embodiments or the description of the prior art.

[0018] Figure 1 This is the effect of the Gypsum Fibrosum polysaccharide extract in the present invention in promoting wound healing in mice. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below with reference to the accompanying drawings and embodiments, but it should not be understood as limiting the scope of protection of the present invention. Any formal equivalent transformation based on the concept of the present invention should be considered as the scope of the present invention.

[0020] This document provides general and / or specific descriptions of the materials and experimental methods used in the experiments. Unless otherwise specified, all experimental or testing methods are conventional methods. All reagents and instruments used, unless the manufacturer is specified, are commercially available, conventional products and were prepared or used using conventional methods.

[0021] In the following embodiments, the gyoza used specifically refers to Haworthia cymbiformis var. obtusa and its variants.

[0022] Example 1: Preparation of Gyokuro polysaccharide extract

[0023] 100 g of fresh Jade Dew leaves were taken, washed, placed in a blender, and 100 mL of deionized water was added, stirred, and crushed into a slurry; deionized water was added to the Jade Dew slurry to make the volume 1000 mL, and ultrasonic extraction was performed at 60° C. for 1 hour; after the extract was cooled, it was centrifuged at 4000 r / min for 10 minutes, and the supernatant was collected; the supernatant was concentrated under reduced pressure at 60° C. to 100 mL; 3 times the volume of 95% ethanol was added to the concentrated solution, and the solution was allowed to stand overnight to precipitate polysaccharides; the precipitate was collected, washed twice with anhydrous ethanol, and vacuum-dried to obtain 1.2 g of Jade Dew polysaccharide extract.

[0024] Example 2: Preparation of a gel containing Gypsum Fibrosum polysaccharide extract

[0025] Weigh 1.0 g of the gyokuro polysaccharide extract prepared in Example 1, add an appropriate amount of deionized water, and stir to fully dissolve it; add 1.0 g of carbomer 940 to the solution, stir evenly, and let it stand overnight to swell; add triethanolamine to adjust the pH to 6.5-7.5, and stir evenly; add 10 g of glycerol and 0.2 g of ethylparaben, stir to dissolve it, and finally add deionized water to 100 g and stir evenly to obtain a gel containing gyokuro polysaccharide extract.

[0026] Example 3: Preparation of wound dressing containing Gypsum Fibrosum polysaccharide extract

[0027] Weigh 0.8 g of the Gypsum glutinosa polysaccharide extract prepared in Example 1, add an appropriate amount of deionized water, and stir to fully dissolve it; add 10 g of chitosan to the solution, stir evenly to fully dissolve it; apply the solution evenly on a non-woven fabric, and dry it at 60°C to obtain a wound dressing containing Gypsum glutinosa polysaccharide extract.

[0028] Experimental Example 1: Verification of the effect of Gyokuro polysaccharide extract in promoting skin wound healing

[0029] Experimental animals: Twenty healthy ICR mice weighing 29-31 g were randomly divided into experimental group 1 and experimental group 2, with 10 mice in each group.

[0030] Modeling: After fasting for 6 hours, mice were anesthetized with 3% to 5% isoflurane, and a circular full-thickness skin incision with a diameter of 8 mm was made on both sides of the back. The wound on the left side of each mouse served as a control, and the wound on the right side was treated with medication.

[0031] Treatment: The wounds of the control group were smeared with normal saline; the wounds of the experimental group 1 were smeared with the gel containing the Gypsum polysaccharide extract prepared in Example 2; the wounds of the experimental group 2 were covered with the wound dressing containing the Gypsum polysaccharide extract prepared in Example 3; the drugs or dressings were changed once a day.

[0032] Observation indicators: Wound healing was observed on the 3rd, 7th and 10th days after surgery (specific results are as follows Figure 1 The wound area was measured and the wound healing rate was calculated: Wound healing rate = (initial wound area - remaining wound area) / initial wound area × 100%.

[0033] Experimental results: On the 3rd day after surgery, the wound healing rates of experimental group 1 and experimental group 2 were 32% and 35%, respectively, which were significantly higher than 13% of the control group (P<0.05); on the 7th day after surgery, the wound healing rates of experimental group 1 and experimental group 2 were 65% and 74%, respectively, which were also significantly higher than 29% of the control group (P<0.05); on the 10th day after surgery, the wounds of experimental group 1 and experimental group 2 were basically healed, with healing rates reaching 92% and 95%, respectively, while the healing rate of the control group was only 47% (P<0.01).

[0034] The above experimental results show that the polysaccharide extract of the present invention has a significant effect in promoting skin wound healing and can be used to prepare medicines, skin care products or dressings that promote skin wound healing.

[0035] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. Application of Gypsum Fibrosum polysaccharide extract in the preparation of preparations for promoting skin wound healing.

2. The application according to claim 1, wherein the polysaccharide extract of Gyokurodes japonica is obtained by the following method: fresh leaves of Gyokurodes japonica are taken, washed, added with an appropriate amount of deionized water and crushed with a blender to obtain Gyokurodes japonica slurry; deionized water is added to the Gyokurodes japonica slurry at a solid-liquid ratio of 1:(1-10), and ultrasonic extraction is performed at 60-80°C for 1-3 hours; after the extract is cooled, it is centrifuged at 4000-6000 r / min for 10-20 minutes, and the supernatant is taken; the supernatant is concentrated under reduced pressure at 60°C to a concentrate with a volume of 1 / 10 of the original solution; 3-5 times the volume of 95% ethanol is added to the concentrate, and the concentrate is allowed to stand overnight to precipitate the polysaccharide; the precipitate is collected, washed with anhydrous ethanol 2-3 times, and vacuum-dried to obtain the Gyokurodes japonica polysaccharide extract.

3. The use according to claim 1, characterized in that The jade dew is grass jade dew and its variants.

4. The use according to claim 1, characterized in that The preparation includes but is not limited to one of a medicine, a dressing or a skin care product.

5. The use according to claim 4, characterized in that The preparation further includes a pharmaceutically acceptable carrier, including but not limited to a diluent, a buffer, a suspension, an emulsion, a granule, an encapsulation agent, an excipient, a filler, an adhesive, a spray, a transdermal absorbent, a wetting agent, a disintegrant, an absorption enhancer, a surfactant, a colorant, a flavoring agent or an adsorption carrier.

6. The use according to claim 1, characterized in that The dosage form of the preparation includes, but is not limited to, tablets, solutions, granules, patches, ointments, capsules, aerosols or suppositories.

7. The use according to claim 1, characterized in that The administration routes of the preparation include, but are not limited to, oral administration, intravenous injection, intramuscular injection, subcutaneous injection, sublingual administration, rectal irrigation, nasal spray, oral spray, topical skin administration or systemic transdermal administration.

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